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Corresponding author: Guadalupe Bribiesca-Contreras ( l.bribiesca-contreras@nhm.ac.uk ) Academic editor: Pavel Stoev
© 2022 Guadalupe Bribiesca-Contreras, Thomas G. Dahlgren, Diva J. Amon, Stephen Cairns, Regan Drennan, Jennifer M. Durden, Marc P. Eléaume, Andrew M. Hosie, Antonina Kremenetskaia, Kirsty McQuaid, Timothy D. O’Hara, Muriel Rabone, Erik Simon-Lledó, Craig R. Smith, Les Watling, Helena Wiklund, Adrian G. Glover.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Bribiesca-Contreras G, Dahlgren TG, Amon DJ, Cairns S, Drennan R, Durden JM, Eléaume MP, Hosie AM, Kremenetskaia A, McQuaid K, O'Hara TD, Rabone M, Simon-Lledó E, Smith CR, Watling L, Wiklund H, Glover AG (2022) Benthic megafauna of the western Clarion-Clipperton Zone, Pacific Ocean. ZooKeys 1113: 1-110. https://doi.org/10.3897/zookeys.1113.82172
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There is a growing interest in the exploitation of deep-sea mineral deposits, particularly on the abyssal seafloor of the central Pacific Clarion-Clipperton Zone (CCZ), which is rich in polymetallic nodules. In order to effectively manage potential exploitation activities, a thorough understanding of the biodiversity, community structure, species ranges, connectivity, and ecosystem functions across a range of scales is needed. The benthic megafauna plays an important role in the functioning of deep-sea ecosystems and represents an important component of the biodiversity. While megafaunal surveys using video and still images have provided insight into CCZ biodiversity, the collection of faunal samples is needed to confirm species identifications to accurately estimate species richness and species ranges, but faunal collections are very rarely carried out. Using a Remotely Operated Vehicle, 55 specimens of benthic megafauna were collected from seamounts and abyssal plains in three Areas of Particular Environmental Interest (APEI 1, APEI 4, and APEI 7) at 3100–5100 m depth in the western CCZ. Using both morphological and molecular evidence, 48 different morphotypes belonging to five phyla were found, only nine referrable to known species, and 39 species potentially new to science. This work highlights the need for detailed taxonomic studies incorporating genetic data, not only within the CCZ, but in other bathyal, abyssal, and hadal regions, as representative genetic reference libraries that could facilitate the generation of species inventories.
Biogeography, deep-sea mining, DNA barcoding, DNA taxonomy, megafauna, polymetallic nodules
The Clarion-Clipperton Zone (CCZ) in the central abyssal Pacific has become of great interest for deep-sea mineral extraction. This large area of abyssal seafloor, approximately 6 million km2 (
During the last few decades, there has been a dramatic increase in the scientific exploration of the CCZ, but our knowledge of the faunal communities associated with nodule fields is still limited, and taxonomic records for the area are scarce (
The CCZ abyssal seafloor is rich in topographic features such as hills, troughs, fracture zones, and seamounts (
Large benthic organisms (benthic megafauna) have been prioritised for monitoring deep-sea ecosystems because they can be studied from seabed imagery (
The DeepCCZ project was conceived to increase our understanding of faunal assemblages and biodiversity in the western CCZ, targeting both unexplored seamounts and APEIs. Here, we provide the first taxonomic synthesis of western CCZ megafauna, which is also the largest megafaunal faunistic study from anywhere in the CCZ based on collected specimens. We provide morphological descriptions, genetic data, and high-resolution imagery for all megafauna specimens collected, including specimens from both the abyssal plains and seamount habitats. It complements similar studies of the high diversity of megafaunal xenophyophores (
The DeepCCZ expedition, aboard the RV Kilo Moana, from 14 May to 16 June 2018, surveyed seamounts and abyssal plains in three Areas of Particular Environmental Interest (APEIs 1, 4, and 7) located in the western Clarion-Clipperton Zone (CCZ; Fig.
Map of the Clarion-Clipperton Zone (top left) indicating the nine Areas of Particular Environmental Interest (APEIs) in red, exploration areas in green, and reserved areas in orange. Shapefiles were sourced from https://www.naturalearthdata.com/downloads/10m-physical-vectors/10m-bathymetry/, and https://www.isa.org.jm/minerals/maps. Detailed maps of the study areas: APEIs 1 (top right), 4 (bottom left), and 7 (bottom right) show bathymetry from satellite values for the entire APEI, and multibeam values obtained during the DeepCCZ expedition. Sites, and specific geoform, where megafauna samples were collected are indicated as yellow stars in abyssal plains, green triangles in seamounts, and pink hexagons in seamount slopes.
Specimens were selected from across as many taxonomic groups as possible, with duplicates of similar morphotypes avoided; thus, the aim was to increase our understanding of megafaunal diversity. A total of 55 specimens were collected during different dives in three APEIs and three different geoforms (abyssal plain, seamount, and seamount slope), 14 specimens were collected from abyssal seafloor in APEI 1; 13 from abyssal seafloor, two from the seamount slope and six from a seamount in APEI 4; and nine from abyssal seafloor and 11 from a seamount in APEI 7 (Fig.
After ROV recovery, specimens were transferred and maintained in cold (2–4 °C), filtered seawater until processed. Following
DNA extraction was performed using the DNeasy Blood and Tissue Kit (Qiagen). The barcode gene cytochrome oxidase I (COI) was the main target because this gene has been used in previous studies on megafauna in the CCZ (e.g.,
Phylogenetic relationships of the western CCZ megafauna were explored by estimating phylogenetic trees for all taxa at different taxonomic levels: phylum Annelida: family Aphroditidae; phylum Arthropoda: order Scalpellomorpha; phylum Cnidaria: order Actiniaria, subclass Ceriantharia, subclass Octocorallia, and class Scyphozoa; phylum Echinodermata: class Asteroidea, class Crinoidea, class Echinoidea, class Holothuroidea, and class Ophiuroidea; and phylum Porifera. Different sets of genes were used depending on published phylogenies and publicly available sequences for each taxon, considering both nuclear and mitochondrial genes if available. For each taxon, sequences were obtained from GenBank (Suppl. material
Phylogenetic trees were estimated using partitioned maximum-likelihood (RAxML v8.2.10;
Taxonomic assignments considered information drawn from both molecular and morphological analyses. For the latter, the collected specimens were sent to expert taxonomists for morphological assignments. We assigned every specimen to the lowest Operational Taxonomic Unit (OTU), each representing a species. However, we took a precautionary approach when assigning species names (
Current records available on OBIS, at a minimum depth of 3000 m, were recovered for each taxon on January 12, 2022 (robis::occurrence;
To gain preliminary insight into connectivity and distributions, morphology of specimens was compared to and, where possible, aligned with a standardised megafauna morphotype catalogue developed from in situ seabed imagery from across the north Pacific abyss, mostly eastern CCZ (Simon-Lledó et al., pers. obs.). The catalogue aligns invertebrate morphotypes, only for specimens larger than 1 cm, encountered in quantitative megafaunal assessments. At the time of writing, the survey areas so far encompassed in the standardised megafauna catalogue are, from east to west: UK-1 (
A total of 55 specimens was collected in the western CCZ (Table
Megafauna specimens collected during the DeepCCZ expedition, including details of their collection such as collection site and geoform (S, seamount; AP, abyssal plain; Sl, seamount slope), substrate or attachment (S, on sediment; E, epibiont, N, nodule; C, crust, Sa, anchored to sediment; B, attached to bone), depth, decimal latitude and longitude, scientific collection and accession number, voucher number, and GenBank accession number.
Classification | Species | Site | Substrate / Attachment | Depth (m) | Coordinates (Latitude, Longitude) | Collection | Accession no. | Voucher | GenBank accession no. |
---|---|---|---|---|---|---|---|---|---|
Annelida Polychaeta Phyllodocida Aphroditidae | Laetmonice stet. CCZ_060 | APEI 7 (S) | S | 3096 | 4.8897, -141.7500 |
|
2022.760 | CCZ_060 | ON400687 (COI) |
Arthropoda Thecostraca Scalpellomorpha Scalpellidae | Trianguloscalpellum gigas | APEI 7 (AP) | E | 4875 | 5.0442, -141.8165 |
|
C74110 | CCZ_074 | ON400698 (COI), ON406624 (18S) |
Catherinum cf. albatrossianum | APEI 7 (AP) | E | 4875 | 5.0442, -141.8165 |
|
C74109 | CCZ_073 | ON400697 (COI), ON406623 (18S) | |
Catherinum cf. novaezelandiae | APEI 1 (AP) | E | 5241 | 11.2751, -153.7444 |
|
C74111 | CCZ_185 | ON400722 (COI), ON406625 (18S) | |
Cnidaria Anthozoa Actiniaria | Metridioidea stet. CCZ_072 | APEI 1 (AP) | E | 4875 | 5.0442, -141.8165 |
|
2021.19 | CCZ_072 | ON400696 (COI) |
Metridioidea stet. CCZ_154 | APEI 4 (AP) | N | 5009 | 6.9702, -149.9426 |
|
2021.27 | CCZ_154 | ON400715 (COI) | |
Metridioidea stet. CCZ_164 | APEI 7 (AP) | E | 5001 | 6.9880, -149.9326 |
|
2021.5 | CCZ_164 | ON400717 (COI) | |
Actinostolidae | Actinostolidae stet. CCZ_183 | APEI 1 (AP) | N | 5241 | 11.2751, -153.7444 |
|
2021.28 | CCZ_183 | ON406626 (18S) |
Actinostolidae stet. CCZ_202 | APEI 4 (AP) | N | 5206 | 11.2518, -153.6059 |
|
2021.22 | CCZ_202 | ON406627 (18S) | |
Scleractinia Caryophyllidae | Fungiacyathus (Fungiacyathus) cf. fragilis | APEI 4 (S) | S | 3562 | 7.2647, -149.7740 |
|
2021.26 | CCZ_107 | NA |
Alcyonacea Chrysogorgiidae | Chrysogorgia sp. CCZ_112 | APEI 4 (Sl) | C | 4125 | 7.2874, -149.8578 |
|
CCZ_112 | ON400711 (COI), ON406602 (16S) | |
Mopseidae | Mopseidae sp. CCZ_088 | APEI 4 (AP) | N | 5018 | 7.0089, -149.9109 |
|
CCZ_088 | ON400705 (COI), ON406603 (16S) | |
Primnoidae | Calyptrophora distolos | APEI 4 (Sl) | C | 4125 | 7.2874, -149.8578 | USNM | 1550968 | CCZ_113 | ON400712 (COI), ON406604 (16S) |
Pennatulacea Protoptilidae | Protoptilum stet. CCZ_068 | APEI 7 (S) | Sa | 3096 | 4.8897, -141.7500 |
|
2021.24 | CCZ_068 | ON400694 (COI), ON406605 (16S) |
Spirularia | Spirularia stet. CCZ_067 | APEI 7 (S) | Sa | 3132 | 4.8875, -141.7572 |
|
2021.23 | CCZ_067 | ON400693 (COI), ON406606 (16S) |
Scyphozoa Somaeostomeae Ulmaridae | Ulmaridae stet. CCZ_069 | APEI 7 (S) | S | 3133 | 4.8876, -141.7572 |
|
2021.25 | CCZ_069 | ON400695 (COI) |
Echinodermata Asteroidea Brisingida Freyellidae | Freyastera cf. tuberculata | APEI 4 (AP) | S | 5000 | 6.9879, -149.9123 |
|
2022.79 | CCZ_087 | ON400704 (COI) |
APEI 4 (AP) | S | 5000 | 6.9873, -149.9331 |
|
2022.80 | CCZ_157 | ON400716 (COI) | ||
Freyastera stet. CCZ_201 | APEI 1 (AP) | S | 5204 | 11.2518, -153.6059 |
|
2022.81 | CCZ_201 | ON400730 (COI) | |
Forcipulatida Zoroasteridae | Zoroaster stet. CCZ_065 | APEI 7 (S) | S | 3132 | 4.8877, -141.7569 |
|
2022.78 | CCZ_065 | ON400691 (COI), ON406607 (16S) |
Crinoidea Comatulida Phrynocrinidae | cf. Porphyrocrinus sp. CCZ_165 | APEI 4 (AP) | N | 5002 | 6.9879, -149.9327 |
|
2022.76 | CCZ_165 | ON400718 (COI), ON406616 (16S) |
Antedonidae | Bathymetrinae incert. CCZ_176 | APEI 4 (AP) | E | 5009 | 6.9879, -149.9326 |
|
2022.77 | CCZ_176 | ON400719 (COI), ON406617 (16S); |
APEI 1 (AP) | E | 5241 | 11.2751, -153.7444 |
|
2022.60 | CCZ_186 | ON400723 (COI), ON406618 (16S) | ||
Echinoidea Aspidodiadematoida Aspidodiadematidae | Plesiodiadema cf. globulosum | APEI 1 (AP) | S | 5204 | 11.2527, -153.5848 | CASIZ | 229305 | CCZ_196 | ON400726 (COI), ON406628 (18S) |
Echinothurioida Kamptosomatidae | Kamptosoma abyssale | APEI 4 (AP) | S | 5040 | 7.0360, -149.9395 | CASIZ | 229306 | CCZ_082 | ON400701 (COI) |
Holothuroidea Persiculida Molpadiodemidae | Molpadiodemas stet. CCZ_102 | APEI 4 (S) | S | 3552 | 7.2701, -149.7827 |
|
2022.66 | CCZ_102 | ON400708 (COI) |
Molpadiodemas stet. CCZ_194 | APEI 1 (AP) | S | 5205 | 11.2517, -153.6055 |
|
2022.71 | CCZ_194 | ON400725 (COI) | |
Synallactida Synallactidae | Synallactes stet. CCZ_153 | APEI 4 (AP) | S | 5009 | 6.9704, -149.9426 |
|
2022.69 | CCZ_153 | ON400714 (COI) |
Synallactidae stet. CCZ_061 | APEI 7 (S) | S | 3132 | 4.8877, -141.7569 |
|
2022.75 | CCZ_061 | ON400688 (COI), ON406640 (18S) | |
Synallactidae stet. CCZ_066 | APEI 7 (S) | S | 3095 | 4.8896, -141.7500 |
|
2022.63 | CCZ_066 | ON400692 (COI), ON406642 (18S) | |
Deimatidae | Oneirophanta stet. CCZ_100 | APEI 4 (S) | S | 3550 | 7.2647, -149.7740 |
|
2022.84 | CCZ_100 | ON400706 (COI), ON406643 (16S), ON406620 (18S) |
Oneirophanta cf. mutabilis | APEI 1 (AP) | S | 5203 | 11.2520, -153.5847 |
|
2021.20 | CCZ_193 | ON400724 (COI), ON406629 (16S), ON406619 (18S) | |
Elasipodida Psychropotidae | Psychropotes verrucicaudatus | APEI 4 (AP) | S | 4999 | 6.9878, -149.9119 |
|
2021.19 | CCZ_086 | ON400703 (COI) |
Psychropotes dyscrita | APEI 4 (AP) | S | 5040 | 7.0212, -149.9355 |
|
2022.83 | CCZ_083 | ON400702 (COI) | |
Benthodytes cf. sanguinolenta | APEI 1 (AP) | S | 5245 | 11.2953, -153.7420 |
|
2022.70 | CCZ_178 | ON400720 (COI) | |
Elasipodida Psychropotidae | Benthodytes marianensis | APEI 7 (AP) | S | 4861 | 5.1043, -141.8865 |
|
2022.82 | CCZ_019 | ON400682 (COI) |
Elpidiidae | Peniagone leander | APEI 7 (AP) | S | 4860 | 5.1042, -141.8861 |
|
2022.61 | CCZ_018 | ON400681 (COI), ON406621 (16S) |
Peniagone vitrea | APEI 7 (AP) | S | 4875 | 5.0442, -141.8164 |
|
2022.64 | CCZ_077 | ON400699 (COI), ON406622 (16S) | |
Laetmogonidae | Psychronaetes sp. CCZ_101 | APEI 4 (S) | S | 3562 | 7.2647, -149.7741 |
|
2022.65 | CCZ_101 | ON400707 (COI), ON406631 (18S) |
APEI 4 (S) | S | 3562 | 7.2647, -149.7741 |
|
2022.68 | CCZ_104 | ON400710 (COI), ON406632 (18S) | ||
APEI 7 (S) | S | 3132 | 4.8877, -141.7570 |
|
2022.62 | CCZ_063 | ON400690 (COI), ON406630 (18S) | ||
APEI 4 (S) | S | 3562 | 7.2647, -149.7741 |
|
2022.67 | CCZ_103 | ON400709 (COI), ON406639 (18S) | ||
Laetmogone cf. wyvillethomsoni | APEI 7 (S) | S | 3132 | 4.8877, -141.7569 |
|
2021.18 | CCZ_062 | ON400689 (COI), ON406641 (18S) | |
Ophiuroidea Ophioscolecida Ophioscolecidae | Ophiocymbium tanyae | APEI 1 (AP) | S | 5204 | 11.2523, -153.5848 |
|
2022.74 | CCZ_206 | ON406633 (18S), ON406596 (28S) |
Ophiocymbium cf. rarispinum | APEI 1 (AP) | S | 5206 | 11.2518, -153.6059 |
|
2022.73 | CCZ_197 | ON400727 (COI) | |
Ophiurida Ophiopyrgidae | Ophiuroglypha cf. irrorata | APEI 7 (S) | S | 3239 | 4.9081, -141.6813 |
|
2021.21 | CCZ_058 | ON400685 (COI) |
APEI 7 (S) | S | 3096 | 4.8897, -141.7500 |
|
2022.72 | CCZ_059 | ON400686 (COI) | ||
Porifera Hexactinellida Amphidiscosida Hyalonematidae | Hyalonema stet. CCZ_020 | APEI 7 (AP) | Sa | 4856 | 5.1149, -141.8967 |
|
CCZ_020 | ON400683 (COI), ON406634 (18S), ON406608 (16S), ON406597 (28S), ON411254 (ALG11) | |
APEI 1 (AP) | Sa | 5245 | 11.2954, -153.7422 |
|
2022.8 | CCZ_179 | ON400721 (COI), ON406609 (16S) | ||
Hyalonema stet. CCZ_081 | APEI 4 (AP) | Sa | 5031 | 7.0360, -149.9395 |
|
2022.9 | CCZ_081 | ON406610 (16S) | |
Lyssacinosida Euplectellidae | Euplectellinae stet. CCZ_199 | APEI 1 (AP) | Sa | 5202 | 11.2518, -153.5853 |
|
CCZ_199 | ON400729 (COI), ON406611 (16S) | |
Docosaccus sp. CCZ_021 | APEI 7 (AP) | Sa | 4860 | 5.1043, -141.8867 |
|
2022.6 | CCZ_021 | ON400684 (COI), ON406635 (18S), ON406612 (16S), ON406598 (28S), ON411255 (ALG11) | |
Lyssacinosida Euplectellidae | Holascus stet. CCZ_078 | APEI 7 (AP) | Sa | 4874 | 5.0443, -141.8162 |
|
2022.7 | CCZ_078 | ON400700 (COI), ON406636 (18S), ON406613 (16S), ON406599 (28S), ON411256 (ALG11) |
Bolosominae stet. CCZ_198 | APEI 1 (AP) | Sa | 5205 | 11.2518, -153.6053 |
|
2022.10 | CCZ_198 | ON400728 (COI), ON406637 (18S), ON406614 (16S), ON406600 (28S) | |
Sceptrulophora Euretidae | Bathyxiphus sp. CCZ_151 | APEI 4 (AP) | B | 5001 | 6.9881, -149.9321 |
|
CCZ_151 | ON400713 (COI), ON406638 (18S), ON406615 (16S), ON406601 (28S) |
Only two of these nine species had been previously found in the CCZ. Juveniles of the brittle star Ophiocymbium tanyae Martynov, 2010 were collected in the eastern IFREMER contract area and in APEI 3, but due to their early life stage, they lacked taxonomically informative characters and were only assigned to family level using DNA barcoding data (
The in situ images taken for 53 specimens were classified into a total of 45 morphotypes using the standardised megafauna imagery catalogue (Simon-Lledó et al., pers. obs.). From these, 11 (24%) were new additions to the existing catalogue, thus representing morphotypes exclusively (to-date) encountered in the western CCZ (i.e., APEIs 1, 4 and 7), while 27 (60%) had already been encountered in other areas. More specifically, nine (20%) of the 45 morphotypes encountered in the western CCZ have also previously been found both in abyssal areas of the Kiribati EEZ (west of the areas studied) and in the eastern CCZ. Two (4%) of the morphotypes encountered in the western CCZ have been found in Kiribati (but not in eastern CCZ locations), whereas 16 (36%) of the western CCZ morphotypes have been encountered in the eastern CCZ, but not in Kiribati.
Phylum Annelida Lamarck, 1809
Class Polychaeta Grube, 1850
Subclass Errantia Audouin & H Milne Edwards, 1832
Order Phyllodocida Dales, 1962
Suborder Aphroditiformia Levinsen, 1883
Family Aphroditidae Malmgren, 1867
Currently, there are no records from ≥ 3000 m depth for the genus Laetmonice Kinberg, 1856, in the Clarion-Clipperton Zone (
Rooted Bayesian phylogeny for the family Aphroditidae. COI-only BEAST median consensus tree with posterior probability (PP) and bootstrap (BS) values indicated for each node. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.
Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8897°N, 141.75°W; 3096 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400687 (COI);
Single specimen (Fig.
Laetmonice stet. CCZ_060 A in situ image B ventral surface C elytra on dorsal surface D harpoon-shaped chaeta E dorsal surface F neurochaeta with fringed tips G notochaetal spine shafts. Scale bars: 2cm (A); 0.5 cm (B, E). Image attribution: Durden and Smith (A), Wiklund, Durden, Drennan, and McQuaid (B, E), Drennan (C, D, F, G).
The presence of harpoon-shaped notochaetae supports the placement of this specimen within the genus Laetmonice (
This specimen was observed crawling on the sedimented seafloor on the seamount of APEI 7 at 3096 m depth.
No exactly identical Aphroditiformia morphotypes have been so far catalogued from seabed imagery collected in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Laetmonice stet. CCZ_060 was added as a new morphotype (i.e., Laetmonice sp. indet., ANN_019) in the megafauna imagery catalogue. Only one other Aphroditiformia morphotype (i.e., Aphroditidae gen. indet., ANN_022; with much larger spines and no sediment coating), was catalogued from seabed imagery in the eastern CCZ, also found on a seamount. In vertically-facing seabed images, Aphroditiformia morphotypes could potentially be confused with plate-shaped Xenophyophore tests (e.g., Psamminidae), particularly a dense layer of sediment is found coating specimens, as observed in Laetmonice stet. CCZ_060 (Fig.
Phylum Arthropoda von Siebold, 1848
Subphylum Crustacea Brünnich, 1772
Superclass Multicrustacea Regier, Shultz, Zwick, Hussey, Ball, Wetzer, Martin & Cunningham, 2010
Class Thecostraca Gruvel, 1905
Subclass Cirripedia Burmeister, 1834
Infraclass Thoracica Darwin, 1854
Superorder Thoracicalcarea Gale, 2015
Order Scalpellomorpha Buckeridge & Newman, 2006
Family Scalpellidae Pilsbry, 1907
To date, there is a single record at > 3,000 m depth for the order Scalpellomorpha in the CCZ (
Rooted Bayesian phylogeny of Scalpellomorpha. Concatenated (18S, and COI) BEAST median consensus tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.
Scalpellomorpha have been commonly found in image-based megafauna surveys across the north Pacific abyss, usually attached to sponge stalks or nodules. However, their classification beyond family level (e.g., Scalpellidae) from seabed imagery is constrained by their generally small size; only large specimens (> 3 cm) which are rarely encountered can sometimes be classified to genus level from in situ images. Consequently, scalpellid specimens usually are collated into a single, generic morphotype (i.e., Scalpellidae gen. indet., ART_010) in image-based quantitative analyses.
Single specimen, found attached to a glass sponge stalk (Fig.
Trianguloscalpellum gigas (Hoek, 1883). Specimen CCZ_074: A in situ photograph, attached to a glass sponge stalk B left C and right lateral views. Scale bars: 5 cm (A); 1 mm (B, C). Image attribution: Durden and Smith (A), Hosie (B, C). Arrows indicate position of T. gigas (specimen CCZ_074; lower, yellow) and Catherinum cf. albatrossianum (specimen CCZ_073; upper, green).
The specimen appears to be a juvenile of the species T. gigas based on the plate arrangement, although diagnostic characters are not fully developed. There are no sequences available on public databases for T. gigas, but the 18S gene sequence is very similar (> 99%) to other species within the family Scalpellidae, mostly within the subfamily Arcoscalpellinae. However, the COI sequence is highly divergent (> 15% nucleotide divergence and > 3% amino-acid divergence) from published sequences of other species within the subfamily. The phylogenetic tree from concatenated data for COI and 18S recovered a well-supported clade of species of Anguloscalpellum and Trianguloscalpellum, but did not recover the genera as monophyletic. The type material for T. gigas was collected during the H.M.S. Challenger expedition in the middle of the North Pacific (Station 246: 36.1667°N, 178.0°E) at 3749 m depth (
The specimen was collected in the sedimented abyssal plain of APEI 7, at 4874 m depth. It was attached to a glass sponge stalk, along with another barnacle (Catherinum cf. albatrossianum; specimen CCZ_073), and an anemone (Metridioidea stet. CCZ_072; specimen CCZ_072).
No exactly similar Scalpellidae morphotypes have been so far catalogued from seabed imagery collected in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Trianguloscalpellum gigas was catalogued as a new morphotype (i.e., Trianguloscalpellum gigas sp. inc., ART_033). However, given the small size of specimen CCZ_074, this morphotype could have easily been i.e., undetected in seabed image surveys conducted in other areas of the CCZ.
Single specimen 21 mm long, attached to glass sponge stalk (Fig.
Morphological characters are in accordance with the description of C. albatrossianum. The 18S sequence matches three genera within the subfamily Arcoscalpellinae Zevina, 1978, while the closest match (85% similarity) for the COI sequence is to another species of Catherinum. Like, C. cf. novaezelandiae it differs morphologically from C. tortilum, reported from the CCZ by
Specimen was collected in a muddy abyssal area of APEI 7, at 4874 m depth. It was attached to a glass sponge stalk (Fig.
A very similar morphotype (Catherinum sp. indet., ART_032) has been encountered (e.g., large specimens > 3 cm in length) in seabed image surveys conducted across the eastern CCZ and in abyssal areas of the Kiribati EEZ.
Single specimen 14 mm long; with elongated, white capitulum, > 2× as long as wide (L = 12 mm, W = 5 mm), and short peduncle (2 mm) with small scales (Fig.
Morphological characters of the capitulum conform to the description of the genus Catherinum. The sequence for the 18S gene is similar to sequences from other species within the same family. Another species within the genus, C. tortilum (Zevina, 1973), originally described from the Indian Ocean at 2760 m depth has also been recorded for the CCZ at similar depths (4872–4877 m depth;
The specimen was collected in the sedimented abyssal plain of APEI 1 at 5241 m depth. It was attached to a glass sponge stalk, along with a crinoid (Bathymetrinae inc. CCZ_176; specimen CCZ_186), a polychaete, and anemones, that was anchored in the mud.
Relatively large abundances of a very similar morphotype (Catherinum sp. indet., ART_031) were observed in seabed imagery collected within abyssal areas of the Kiribati EEZ, but not in eastern CCZ surveys.
Phylum Cnidaria Hatschek, 1888
A total of 12 cnidarians w collected, belonging to six orders in two classes (Anthozoa and Scyphozoa).
Class Anthozoa Ehrenberg, 1834
Subclass Hexacorallia Haeckel, 1896
Order Actiniaria Hertwig, 1882
To date, there are 33 records of Actiniaria found at > 3000 m depth in the CCZ (
Rooted Bayesian phylogeny of Actiniaria. Concatenated (12S, 16S, 18S, 28S, COI, and COX3) BEAST median consensus tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.
Suborder Enthemonae Rodríguez & Daly in Rodríguez et al. 2014
Superfamily Metridioidea Carlgren, 1893
Metridioidea stet. CCZ_072
Fig.
Material. Clarion-Clipperton Zone • 1 specimen; APEI 1; 5.0442°N, 141.8165°W; 4875 m deep; 28 May. 2018; Smith & Durden leg.; GenBank: ON400696 (COI);
Description. Single specimen, white (Figs
Remarks. COI sequence is similar (97.3%) to other species within the subfamily Metridioidea but based on COI we were unable to delimit species because interspecific divergence is very low. Additionally, only a few studies have included sequences for COI, therefore hindering comparisons based solely on this gene. The COI divergence between Metridiodea stet. CCZ_164 and Metridiodea stet. CCZ_072 (1.95% K2P distance) was higher than the genetic distance between other species in the family Metridiodea (
Ecology. The specimen was collected in a muddy abyssal plain in APEI 7, at 4874 m depth. It was attached to a glass sponge stalk (Fig.
Comparison with image-based catalogue. A very similar Actiniaria morphotype (Metridioidea fam. indet., ACT_042) mostly attached to sponge stalks, has been commonly encountered in seabed image surveys conducted across the eastern CCZ but not in abyssal areas of the Kiribati EEZ.
Metridioidea stet. CCZ_154
Fig.
Material. Clarion-Clipperton Zone • 1 specimen; APEI 4; 6.9702°N, 149.9426°W; 5009 m deep; 06 Jun. 2018; Smith & Durden leg.; GenBank: ON400715 (COI);
Description. Single specimen, completely white when alive (Fig.
Ecology. This specimen was attached to a nodule in abyssal sediments in APEI 4 at 5009 m depth.
Remarks. The COI sequence is similar to sequences of species within different families, but in the phylogenetic tree it is recovered within the superfamily Metridioidea (Fig.
Comparison with image-based catalogue. No similar Actiniaria morphotypes had been catalogued so far from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. The in situ image of Metridiodea stet. CCZ_154 was hence catalogued as a new morphotype (i.e., Metridioidea fam. indet., ACT_044).
Metridioidea stet. CCZ_164
Fig.
Material. Clarion-Clipperton Zone • 1 specimen; APEI 7; 6.988°N, 149.9326°W; 5001 m deep; 06 Jun. 2018; Smith & Durden leg.; GenBank: ON400717 (COI);
Description. Single specimen, white (Fig.
Remarks. COI sequence is very similar to Metridiodea sp. CCZ_072 and they are recovered as sister species, in the multi-gene phylogeny, within the Cuticulata in the superfamily Metridioidea (Fig.
Ecology. This specimen was collected in muddy abyssal sediments in APEI 4 at 5001 m depth, attached to a glass sponge stalk.
Comparison with image-based catalogue. As with specimen from Metridiodea stet. CCZ_072, a very similar morphotype has been commonly found in seabed image surveys conducted across the eastern CCZ (i.e., Metridioidea fam. indet., ACT_042), but it does not seem possible to differentiate between the species Metridiodea stet. CCZ_072 and Metridiodea stet. CCZ_164 from in situ imagery. Morphotype ACT_042 is hence likely to encompass, at least, these two species in image-based analyses conducted across the CCZ.
Superfamily Actinostoloidea Carlgren, 1932
Family Actinostolidae Carlgren, 1932
Actinostolidae stet. CCZ_183
Fig.
Material. Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2751°N, 153.7444°W; 5241 m deep; 09 Jun. 2018; Smith & Durden leg.; GenBank: ON406626 (18S);
Description. Single specimen, white, attached to a nodule (Fig.
Remarks. Closest matches for the 18S sequence are sequences from other members of the family Actinostolidae (> 99.3%). In the phylogenetic tree, it is also recovered in a well-supported clade with species of the family Actinostolidae (Fig.
Ecology. This specimen was collected in abyssal sediment in APEI 1 at 5241 m depth, attached to a polymetallic nodule.
Actinostolidae stet. CCZ_202
Fig.
Material. Clarion-Clipperton Zone • 1 specimen; APEI 4; 11.2518°N, 153.6059°W; 5206 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON406627 (18S);
Description. Single, white specimen (Fig.
Remarks. The closest matches to the 18S sequence are species in different suborders within the Actiniaria (98.3% sequence similarity), including Hormathiidae sp. NHM_416 from the CCZ (
Ecology. This specimen was attached to a polymetallic nodule collected in abyssal sediments of APEI 1 at 5206 m depth.
Comparison with image-based catalogue. No similar Actiniaria morphotypes have been so far catalogued from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. The in situ image of Actinostolidae stet. CCZ_202 was hence catalogued as a new morphotype (i.e., Actinostolidae gen. indet., ACT_080). However, small actiniarians (e.g., oral disc < 2 cm) are usually difficult to classify from seabed imagery as basic morphological features (e.g., number of tentacles) are often not clearly visible. Consequently, ACT_080 could be potentially confused with similarly small actinian morphotypes commonly encountered in the eastern CCZ (i.e., Hormathiidae gen. inc., ACT_022, also with a short pedal approx. the same diameter as the oral disc, but with 16–18 long thin tentacles).
Order Scleractinia Bourne, 1900
For Scleractinia, there are only two records at > 3000 m depth in the CCZ (
Family Fungiacyathidae Chevalier & Beauvais, 1987
Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.2647°N, 149.774°W; 3562 m deep; 03 Jun. 2018; Smith & Durden leg.;
Single specimen, solitary, and unattached, ~ 27 mm in transverse diameter. Live specimen with tapered, transparent tentacles, longer than half the corallum diameter and arranged in two or three cycles (Fig.
No genetic sequences were obtained from this specimen. Morphological characters match the genus Fungiacyathus.
This free-living specimen was found on a sedimented area on a seamount in APEI 4, at 3561 m depth.
A very similar scleractinian morphotype (i.e., Fungiacyathus sp. indet., SCL_003) has been encountered in seabed image surveys conducted across the eastern CCZ but not in abyssal areas of the Kiribati EEZ, usually on sediment. As with other solitary scleractinians, this taxon could be confused with an anemone in seabed imagery (e.g., SCL_003 was originally catalogued as an Actiniaria from in situ images, which was addressed following the collection and analysis of the specimen collected in this study).
Subclass Octocorallia Haeckel, 1866
Order Alcyonacea Lamouroux, 1812
There are 131 records of Alcyonacea at > 3000 m depth in the CCZ, only eight of those representing preserved specimens (
Rooted Bayesian phylogeny of Octocorallia. Concatenated (16S, COI, mtMutS, NADH2) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.
Suborder Calcaxonia Grasshoff, 1999
Family Chrysogorgiidae Verrill, 1883
Wide, long, sparsely branched colony, ~ 30 cm tall from the base (Fig.
Chrysogorgia sp. CCZ_112 A, B in situ images of colony C closed polyp on live specimen D opened polyp on live specimen showing light orange colouration E closed polyp of preserved specimen. Scale bars: 2 cm (A); 0.5 mm (C–E). Image attribution: Durden and Smith (A, B); Wiklund, Durden, Drennan, and McQuaid (C, D); Bribiesca-Contreras (E).
The sequence for the COI gene is 0% divergent from a sequence of a specimen of Chrysogorgia abludo Pante & Watling, 2011 (specimen NAS102-3, GenBank accession number GQ180138) collected at Nashville Seamount, New England Seamounts at 2246 m depth (Station 102; 34.5828°N, 56.8433°W) included as comparative material during the species description (
No similar Alcyonacea morphotypes have been catalogued so far from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Chrysogorgia sp. CCZ_112 was catalogued as a new morphotype (i.e., Chrysogorgia sp. indet., ALC_017).
The specimen was attached to polymetallic crust on the slope of a seamount in the APEI 4, at 4124 m depth.
Family Mopseidae Gray, 1870
Mopseidae sp. CCZ_088
Fig.
Material. Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.0089°N, 149.9109°W; 5018 m deep; 02 Jun. 2018; Smith & Durden leg.; GenBank: ON400705 (COI), ON406603 (16S);
Description. Single specimen, with white axis and polyps; polyps standing perpendicular to the axis when alive (Fig.
Remarks. Both 16S (0.3% K2P) and COI (0.6% K2) sequences are very similar to Mopseinae sp. NHM_330 (
Ecology. The specimen was found attached to a nodule in abyssal sediments of APEI 4 at 5018 m depth.
Comparison with image-based catalogue. No similar Alcyonacea morphotypes had been catalogued so far from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of CCZ_088 was catalogued as a new morphotype (i.e., Mopseidae gen. indet., ALC_018). However, it is often not possible to determine whether such small and abundant polyps are arranged in pairs or not, or the actual orientation of these with regards to the axis from seabed images.
Family Primnoidae Milne Edwards, 1857
Branching uniplanar, colony ~ 20.8 cm tall, with polyps perpendicular to the stem in in situ images (Fig.
Morphological characters are concordant with the description of Calyptrophora distolos (
The specimen was found attached to a polymetallic crust on the slope of a seamount on APEI 4, at 4124 m depth.
A similar primnoid morphotype (i.e., Calyptrophora distolos sp. inc., ALC_016) was catalogued from seabed imagery (also collected on a seamount) in the eastern CCZ (e.g.,
Order Pennatulacea Verrill, 1865
A total of 79 records of Pennatulacea occurring at > 3000 m depth in the CCZ have been recorded in OBIS, but none represent preserved specimens (
Suborder Sessiliflorae Kükenthal, 1915
Family Protoptilidae Kölliker, 1872
Single specimen, ~ 12 cm tall, narrow sea pen; in situ colouration orange with whitish polyps (Fig.
The COI sequence forms a clade with sequences from Protoptilum (< 1% genetic divergence), a genus within the family Protoptilidae, while the 16S sequence is very similar to sequences of Protoptilum and Distichoptilum, both genera within the same family. In the phylogenetic tree, the family Protoptilidae was not recovered as monophyletic, but the CCZ specimen was recovered (with 1.00 posterior probability) as sister to Protoptilum carpenterii Kölliker, 1872.
The specimen was found anchored to soft sediment on a seamount of APEI 7, at 3096 m depth.
No similar Pennatulacea morphotypes have been catalogued so far from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Protoptilum stet. CCZ_068 was catalogued as a new morphotype (i.e., Protoptilum sp. indet., PEN_024). In seabed images, PEN_024 can resemble other single-branched sea pens or even soft corals.
Subclass Ceriantharia Perrier, 1893
Order Spirularia den Hartog, 1977
To date, there are no records from a minimum of 3000 m depth in the CCZ for the order Spirularia (
Rooted Bayesian phylogeny of Ceriantharia. Concatenated (12S, 16S, 18S, 28S, and COI) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.
Spirularia stet. CCZ_067
Fig.
Material. Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8875°N, 141.7572°W; 3132 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400693 (COI), ON406606 (16S);
Description. Single specimen, unattached, tube-dweller with tentacles extended above the sediment in situ (Fig.
Remarks. The closest matches to the COI and 16S sequences were sequences from other members of the family Cerianthidae: Pachycenrianthus, Cerianthus, Ceriantheromorphe. However, in the concatenated phylogeny, it forms a clade with Boctrunidifer sp. 1 and Ceriantheopsis americanus, belonging to the families Botrucnidiferidae and Cerianthidae, respectively (Fig.
Ecology. The specimen was found buried in the sediment on a seamount in APEI 7, at 3132 m depth.
Comparison with image-based catalogue. A very similar Ceriantharia morphotype (i.e., Spirularia sp. indet., CER_001) has been commonly encountered in seabed image surveys conducted across the eastern CCZ, always found semi-buried with the tentacles extending above the sediment surface.
Class Scyphozoa Goette, 1887
For the class Scyphozoa, there are currently 128 records from > 3000 m depth in the CCZ, but none represent preserved specimens (
Rooted Bayesian phylogeny of Scyphozoa. Concatenated (16S, 18S, 28S, and COI) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.
Subclass Discomedusae Haeckel, 1880
Order Somaeostomeae Agassiz, 1862
Family Ulmaridae Haeckel, 1880
Ulmaridae stet. CCZ_069
Fig.
Material. Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8876°N, 141.7572°W; 3133 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400695 (COI);
Description. Single specimen, ~ 4.5 cm in diameter; with transparent bell and light brown tentacles in situ (Fig.
Remarks. Only the sequence for the COI gene was successfully amplified, but none of the matches on public databases were informative. In the phylogenetic tree (Fig.
Ecology. The specimen was found on the sediment of a seamount in APEI 7 at 3095–3132 m depth. A similar species of ulmariid from the New Britain Trench was found to skim the seafloor to feed on particulates on the sediment (
Comparison with image-based catalogue. No similar Ulmaridae morphotypes have been catalogued so far from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Ulmaridae stet. CCZ_069 was catalogued as a new morphotype (i.e., Ulmaridae gen. indet., SCY_010). A similarly shaped Ulmaridae morphotype (e.g., Ulmaridae gen. indet., SCY_009; opaque reddish bell, dark brown tentacles encircled with a white ring, and dark rhopalia around the bell), also eventually found crawling on the seabed surface, was previously catalogued from seabed imagery in nodule field areas of the eastern CCZ. When photographed lying on the seabed (as opposed to swimming in the water column), SCY_019 and SCY_010 may resemble an anemone, particularly in images collected at high altitude above the seabed (e.g., > 5 m).
Phylum Echinodermata
Class Asteroidea de Blainville, 1830
There are currently 245 records of sea stars occurring at a minimum of 3000 m depth in the CCZ, with only five of those representing preserved specimens (
Rooted Bayesian phylogeny of Asteroidea. Concatenated (12S, 16S, 18S, COI, and H3) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.
Superorder Forcipulatacea Blake, 1987
Order Brisingida Fisher, 1928
Family Freyellidae Downey, 1986
Clarion-Clipperton Zone • 1 specimen; APEI 4; 6.9879°N, 149.9123°W; 5000 m deep; 02 Jun. 2018; Smith & Durden leg.; GenBank: ON400716 (COI);
Pacific Ocean • 1 specimen, holotype of Freyella benthophila Sladen, 1889; mid-South Pacific; 39.6833°S, 131.3833°W; 4663 m deep; Challenger Expedition, Stn. 289;
Two specimens (R = 106 mm, r = 3 mm; R = 164 mm, r = 6 mm); live specimens whitish on both actinal and abactinal surfaces, tube feet transparent with bright orange flattened discs (Fig.
Freyastera cf. tuberculata (Sladen, 1889). Specimen CCZ_175: A in situ image C whole specimen D ventral surface of the arms before preservation. Specimen CCZ_087: B in situ image E details of dorsal disc F ventral disc surface before preservation. Scale bars: 2 cm (A, B); 1 cm (C); 2 mm (D); 1 mm (E); 0.5 mm (F). Image attribution: Durden and Smith (A, B); Wiklund, Durden, Drennan, and McQuaid (C–F).
The COI sequences were very similar to sequences of Freyastera cf. benthophila (Sladen, 1889) collected in the UK-1 contract area from the CCZ (
One specimen was observed on the sedimented seafloor (CCZ_157), while another was sitting on a nodule with the actinal surface against the muddy seafloor and lifting the tip of the arms like a basket (CCZ_087). Both seastars were collected on abyssal sediments of APEI 4 at 5000 m depth. During morphological examination of these samples, the exoskeleton of a large (> 6 mm long), digested copepod was found in the stomach of specimen CCZ_157.
Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2518°N, 153.6059°W; 5204 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON400730 (COI);
Pacific Ocean • 1 specimen, holotype of Freyella benthophila Sladen, 1889; mid-South Pacific; 39.6833°S, 131.3833°W; 4663 m deep; Challenger Expedition, Stn. 289;
Single specimen, with very small disc and six long, slender, tapered arms (R = 190 mm, r = 5 mm; Fig.
The COI sequence is 4% divergent from the two specimens of Freyastera cf. tuberculata reported herein, and hence considered a separate species. It is also divergent (> 4% K2P distance) to sequences of other species of Freyastera, but forms a monophyletic clade with those, confirming its placement within the genus (Fig.
The specimen was collected on the sedimented abyssal plain of APEI 1 at 5204 m depth, with arms curled up like a basket (Fig.
Freyastera spp. are commonly found in image-based megafauna assessments across the CCZ (e.g.,
Order Forcipulatida Perrier, 1884
Family Zoroasteridae Sladen, 1889
Single specimen (R = 16.6 cm, r = 1.3 cm). Actinal and abactinal surfaces are bright orange when alive, with ambulacrum slightly darker orange (Fig.
Morphological characters are concordant with the description of the genus Zoroaster. The phylogenetic analyses also recovered the specimen in a well-supported clade with other species of the genus (Fig.
The specimen was found partially buried in the sediment on the seamount on APEI 7 at 3133 m depth.
No similar Zoroasteridae morphotypes have been catalogued so far from seabed imagery in the eastern CCZ nor in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Zoroaster stet. CCZ_065 was catalogued as a new morphotype (i.e., Zoroaster sp. indet., AST_025).
Class Crinoidea
To date, there are 66 records of crinoids occurring deeper than 3000 m in the CCZ, with only seven of these representing preserved specimens (
Rooted Bayesian phylogeny of Crinoidea. Concatenated (16S, 18S, 28S, COI, and CytB) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.
Subclass Articulata Zittel, 1879
Order Comatulida
Suborder Bourgueticrinina Sieverts-Doreck, 1953
Family Phrynocrinidae AH Clark, 1907
Subfamily Porphyrocrininae AM Clark, 1973
Single specimen, attached to a nodule by a xenomorphic stalk (Fig.
Morphological characters are concordant with those of the family Phrynocrinidae and the genus Porphyrocrinus as understood by
The specimen was found attached to a nodule in the abyssal sediments of APEI 4 at 5001 m depth.
No similar Comatulida morphotypes have been catalogued so far from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of CCZ_065 was catalogued as a new morphotype (i.e., Porphyrocrinus sp. indet., CRI_008). Note however, that the in situ image of CCZ_065 was collected from an oblique angle and zoomed-in camera, generating a detailed view of a specimen that, owing to its small size, would be otherwise difficult to identify in quantitative assessments, e.g., where images are usually collected vertically-facing, fully zoomed out, and at a higher altitude above the seabed.
Superfamily Antedonoidea Norman, 1865
Family Antedonidae Norman, 1865
Subfamily Bathymetrinae AH Clark, 1909
Bathymetrinae inc. CCZ_176
Fig.
Material. Clarion-Clipperton Zone • 1 adult specimen; APEI 4; 6.9879°N, 149.9326°W; 5009 m deep; 06 Jun. 2018; Smith & Durden leg.; GenBank: ON400719 (COI), ON406617 (16S);
Description. Two specimens, one adult (CCZ_176; Fig.
Bathymetrinae inc. CCZ_176 A side view of adult specimen. Specimen CCZ_186 B pentacrinoid stage attached to a glass sponge stalk C pentacrinoid stage. Scale bars: 5 mm (A); 1 mm (B); 0.5 mm (C). Image attribution: Wiklund, Durden, Drennan, and McQuaid (A, B); Bribiesca-Contreras 2019 (C).
Remarks. Morphological characters are concordant with those of the subfamily Bathymetrinae in the family Antedonidae. The closest match (2.7% K2P) to the COI sequences is a sequence of Psathyrometra fragilis (AH Clark, 1907) from Rodriguez Seamount (1887 m; SIO-BIC E4433), within the family Zenometridae. However, in the phylogenetic analysis the specimens were recovered in a different clade from Psathyrometra spp. (Fig.
Ecology. The adult specimen was found attached to a glass sponge stalk (Fig.
Comparison with image-based catalogue. A very similar Comatulida morphotype (i.e., Bathymetrinae gen. indet., CRI_001) has been commonly encountered in seabed image surveys conducted across the eastern CCZ, both in nodule fields and in seamount areas (
Class Echinoidea
To date, there are 1455 records of echinoids occurring deeper than 3000 m in the CCZ, 11 of these representing preserved specimens (
Phylogenetic tree of Echinoidea. COI-only median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.
Subclass Euechinoidea Bronn, 1860
Infraclass Audolonta Jackson, 1912
Superoder Echinothuriacea Jensen, 1982
Order Aspidodiadematoida Kroh & Smith, 2010
Family Aspidodiadematidae Duncan, 1889
Single specimen, with a somewhat spherical, slightly flattened test (d = 2 cm, H = 1.5 cm). In situ colouration is purple, but the inflated anal cone is greyish blue (Fig.
In 1980, the RV Governor Ray collected several Aspidodiadematidae specimens in the CCZ at ~ 4,800 m, and were assigned to the species P. globulosum. The type localities of P. globulosum are the north of Malpelo Island, and from off Galera Point, Ecuador in the Pacific Ocean, from 2877 to 3241 m depth (
The specimen was collected on the sedimented abyssal plain of APEI 1, at 5203 m depth.
A very similar Plesiodiadema sp. morphotype (i.e., Plesiodiadema globulosum sp. inc., URC_003) has been commonly found in image-based megafauna assessments conducted in the eastern CCZ (e.g.,
Order Echinothurioida Claus, 1880
Family Kamptosomatidae Mortensen, 1934
Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.036°N, 149.9395°W; 5040 m deep; 01 Jun. 2018; Smith & Durden leg.; GenBank: ON400701 (COI); CASIZ 229306; Voucher code: CCZ_082.
Pacific Ocean • 1 specimen, holotype of Kamptosoma asterias (A. Agassiz); off the coast of Chile; 33.5167°S, 74.7167°W; 3950 m deep; Challenger Expedition, Stn. 299;
Single specimen (d = 3.4 cm, H = 1.6 cm). In situ, the body is reddish brown, rounded and flattened (Fig.
Only two species of Kamptosoma have been described to date. Kamptosoma asterias (Agassiz, 1881) was first described from off the coast of Chile at 3950 m depth (type locality: H.M.S Challenger St. 299), and from the east of Malden Island, Central Pacific, at 4750 m depth (type locality for K. indistinctum synonymous with K. asterias: H.M.S. Challenger St. 272) (
The specimen was found crawling rapidly across abyssal sediment in APEI 4, at 5040 m depth. This morphotype has an unusually high crawling speed.
A very similar Kamptosoma sp. morphotype (i.e., Kamptosoma abyssale sp. inc., URC_010) has been encountered in seabed image surveys conducted in abyssal areas of Kiribati’s EEZ, but not in the eastern CCZ. URC_010 was the most abundant echinoid morphotype encountered in the abyssal areas explored within Kiribati’s EEZ (
Class Holothuroidea
Holothurians are important components of the benthic deep-sea megafauna, and currently there are 367 records at a minimum depth of 3000 m in the CCZ, with 141 representing preserved specimens (
Phylogenetic tree of the class Holothuroidea. Concatenated (12S, 16S, 18S, 28S, COI, and H3) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.
Subclass Actinopoda Ludwig, 1891
Order Persiculida Miller, Kerr, Paulay, Reich, Wilson, Carvajal & Rouse, 2017
Family Molpadiodemidae Miller, Kerr, Paulay, Reich, Wilson, Carvajal & Rouse, 2017
Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.2701°N, 149.7827°W; 3552 m deep; 03 Jun. 2018; Smith & Durden leg.; GenBank: ON400708 (COI);
Single specimen, ~ 32 cm long (Fig.
Molpadiodemas stet. CCZ_102 A in situ image B tentacle ossicles C tube feet D dorsal surface E ventral surface of specimen before preservation. Scale bars: 2 cm (A); 20 μm (B); 1 cm (D, E). Image attribution: Durden and Smith (A); Bribiesca-Contreras (B, C); Wiklund, Durden, Drennan, and McQuaid (D, E).
COI sequence forms a clade with other species of Molpadiodemas including M. villosus (Théel, 1886), M. morbillus O’Loughlin & Ahearn, 2005, M. crinitus O’Loughlin & Ahearn, 2005 and M. involutus (Sluiter, 1901). Closest is to M. morbilus (K2P 3.7–3.9%), and in the phylogenetic tree it is recovered in a well-supported clade with other species within the genus (Fig.
This specimen was collected on the sediment seafloor of a seamount in APEI 4 at 3552 m depth.
A very similar Molpadiodemidae morphotype (i.e., Molpadiodemas sp. indet., HOL_103) has been commonly encountered in seabed image surveys conducted across the eastern CCZ (e.g.,
Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2517°N, 153.6055°W; 5205 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON400725 (COI);
Single specimen (Fig.
Molpadiodemas stet. CCZ_194 A in situ image B tentacle ossicles C epibionts on ventral surface D detail of epibionts E dorsal surface F ventral view of specimen before preservation. Scale bars: 2 cm (A); 25 μm (B); 1 cm (E, F). Image attribution: Durden and Smith (A); Bribiesca-Contreras (B–D); Wiklund, Durden, Drennan, and McQuaid (E, F).
The COI sequence of Molpadiodemas stet. CCZ_194 is similar to sequences of other species of Molpadiodemas, including M. villosus, M. morbillus, M. crinitus, M. involutus, and Molpadiodemas stet. CCZ_102. COI genetic divergence between both specimens collected in the CCZ is 6%, in accordance with values of genetic interspecific divergence for the genus. The specimen is recovered in a well-supported clade along with other members of the genus (Fig.
This specimen was found on the sedimented seafloor of an abyssal plain on APEI 1 at 5205 m depth.
A very similar Molpadiodemas sp. morphotype (i.e., Molpadiodemas sp. indet., HOL_004) has been commonly encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ (e.g.,
Order Synallactida Miller, Kerr, Paulay, Reich, Wilson, Carvajal & Rouse, 2017
Family Synallactidae Ludwig, 1894
Synallactidae stet. CCZ_061
Fig.
Material. Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8877°N, 141.7569°W; 3132 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400688 (COI), ON406640 (18S);
Description. Single specimen; description of external morphological features only from in situ image as the specimen was damaged during collection (Fig.
Remarks. There are no close matches to the COI sequence of Synallactidae stet. CCZ_061 in public databases. The closest match is to Synallactidae stet. CCZ_066 (14% K2P). Both specimens are recovered in a well-supported clade representing the family Synallactidae (Fig.
Comparison with image-based catalogue. No similar Synallactidae morphotypes have been so far catalogued from seabed imagery collected in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Synallactidae stet. CCZ_061 was catalogued as a new morphotype (i.e., Synallactidae gen. indet., HOL_120).
Ecology. This specimen was collected on the sedimented seafloor of a seamount in APEI 7 at 3132 m depth.
Synallactidae stet. CCZ_066
Fig.
Material. Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8896°N, 141.75°W; 3095 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400692 (COI), ON406642 (18S);
Description. Single specimen, body semi-circular with ventral surface flattened; ~ 3× longer than wide (L = 21 cm, W = 6 cm; Fig.
Synallactidae stet. CCZ_066 A in situ image B ossicles on dorsal skin C tentacle ossicles D dorsal surface E ventral surface of specimen before preservation. Scale bars: 2 cm (A, D, E); 50 μm (B, C). Image attribution: Durden and Smith (A); Bribiesca-Contreras (B, C); Wiklund, Durden, Drennan, and McQuaid (D, E).
Remarks. There are no close matches to the COI sequence of Synallactidae stet. CCZ_066 in public databases. The closest match is to specimen Synallactidae stet. CCZ_061 (14% K2P). Both specimens are recovered in a well-supported clade representing the family Synallactidae (Fig.
Ecology. This specimen was collected on the sedimented seafloor of a seamount on APEI 7 at 3095 m depth.
Comparison with image-based catalogue. No similar Synallactidae morphotypes have been so far catalogued from seabed imagery collected in the eastern CCZ nor in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of CCZ_066 was catalogued as a new morphotype (i.e., Synallactidae gen. indet., HOL_121). The dorsal protrusions that differentiate HOL_121 from HOL_120 may not be clearly visible in vertically-facing seabed imagery, and hence these two taxa might only be classifiable into a single, generic morphotype (i.e., HOL_120) in quantitative analyses.
Clarion-Clipperton Zone • 1 specimen; APEI 4; 6.9704°N, 149.9426°W; 5009 m deep; 06 Jun. 2018; Smith & Durden leg.; GenBank: ON400714 (COI);
Single specimen (Fig.
Synallactes stet. CCZ_153 A in situ image B ossicles from dorsal skin C ossicles from ventral skin D dorsal view of specimen before preservation, E ventral view. Scale bars: 2 cm (A); 50 μm (B, C); 5 mm (D, E). Image attribution: Durden and Smith (A); Bribiesca-Contreras (B, C); Wiklund, Durden, Drennan, and McQuaid (D, E).
The closest matches for the barcoding gene COI sequence are published sequences from the genus Bathyplotes (89.9% similarity), also within the family Synallactidae. The sequence is distinct from the only sequence of Synallactes sp. (GenBank accession number: KX874365.1) included in the phylogeny (Fig.
This specimen was found on the sedimented seafloor of an abyssal plain on APEI 4 at 5008 m depth.
A very similar Synallactidae morphotype (i.e., Synallactes sp. indet., HOL_007) has been commonly encountered in seabed image surveys conducted across nodule field areas of the eastern CCZ (e.g.,
Family Deimatidae Théel, 1882
Single specimen; colouration of live specimen is beige, spotted with light brown and yellow on dorsal surface (Fig.
Oneirophanta stet. CCZ_100 A dorsal view of specimen before preservation B in situ image C ventral view D dorsal ossicles E ventral ossicles. Scale bars: 2 cm (A, C); 5 cm (B); 200 μm (D); 100 μm (E). Image attribution: Wiklund, Durden, Drennan, and McQuaid (A, C); Durden and Smith (B); Kremenetskaia (D, E).
Closest match for COI and 16S sequences is to Oneirophanta setigera (Ludwig, 1893) (86.7% and 96.3%, respectively). In the phylogenetic tree, it is recovered in a well-supported clade representing the family Deimatidae, including Oneirophanta (Fig.
The specimen was found on the sediment seafloor of a seamount on APEI 4 at 3550 m depth.
No exactly similar Deimatidae morphotypes have been so far catalogued from seabed imagery collected in the eastern CCZ nor in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of CCZ_100 was catalogued as a new morphotype (i.e., Oneirophanta sp. indet., HOL_063). However, HOL_063 could be potentially confused with a similar shaped Deimatidae morphotype (e.g., Deimatidae gen. indet., HOL_062; also beige, cylindrical, with conspicuous projections on the dorsal surface arranged in four rows) found in the eastern CCZ (e.g.,
Indian Ocean • 3 specimens, syntypes of Oneirophanta mutabilis Théel, 1879; Eastern Indian, Antarctic Basin; 53.9167° S, 108.5833° E; 3566 m deep; Challenger Expedition, Stn. 157;
Single specimen, body uniformly white (Fig.
Oneirophanta cf. mutabilis Théel, 1879. Specimen CCZ_193 A in situ image B dorsal ossicles C dorsal view before preservation D ventral view. Scale bars: 200 μm (B); 1 cm (C, D). Image attribution: Durden and Smith (A); Bribiesca-Contreras (B); Wiklund, Durden, Drennan, and McQuaid (C, D).
Sequences for the 18S, 16S, and COI genes were most similar to sequences from Oneirophanta setigera (99.07%, 95.6%, 88.51% similarity, respectively), followed by other species within the family Deimatidae (i.e., Orphnurgus glaber Walsh, 1891 and Deima validum Théel, 1879). The specimen was recovered in a well-supported clade including all members of Deimatidae (Fig.
The specimen was found on the sediment surface of an abyssal plain on APEI 1 at 5203 m depth.
A very similar Oneirophanta sp. morphotype (i.e., Oneirophanta sp. indet., HOL_058) has been encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ (e.g.,
Order Elasipodida Théel, 1882
Family Psychropotidae Théel, 1882
Clarion-Clipperton Zone • 1 specimen; APEI 4; 6.9878°N, 149.9119°W; 4999 m deep; 02 Jun. 2018; Smith & Durden leg.; GenBank: ON400703 (COI);
Single specimen, colouration in situ is violet (Fig.
Psychropotes verrucicaudatus Xiao, Gong, Kou, Li, 2019. Specimen CCZ_086: A, B in situ images C dorsal view of specimen before preservation D ventral view E dorsal ossicles F detail of warts and ossicles on dorsal body wall G mouth tentacles. Scale bars: 5 cm (B); 2 cm (C, D); 100 μm (E). Image attribution: Durden and Smith (A, B); Wiklund, Durden, Drennan, and McQuaid (C, D); Bribiesca-Contreras (E–G).
COI sequence is very similar (K2P distance = 0.77%) to the holotype of P. verrucicaudatus, and they were recovered together in the phylogenetic tree (Fig.
The specimen was found on the sedimented abyssal plain in APEI 4 at 4999 m depth.
A very similar Psychropotes sp. morphotype (i.e., Psychropotes verrucicaudatus sp. inc., HOL_045) has been commonly encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ (e.g.,
Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.0212°N, 149.9355°W; 5040 m deep; 02 Jun. 2018; Smith & Durden leg.; GenBank: ON400702 (COI);
Single specimen, ~ 30 cm long (Fig.
The specimen was found on the sediment seafloor of an abyssal plain in APEI 4 at 5040 m depth.
A very similar Psychropotes sp. morphotype (i.e., Psychropotes sp. indet., HOL_047) has been encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ (e.g.,
Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2953°N, 153.742°W; 5245 m deep; 09 Jun. 2018; Smith & Durden leg.; GenBank: ON400720 (COI);
Single specimen (Fig.
The closest match for the COI sequence is a sequence from B. sanguinolenta (GenBank: HM196505.1; 93.54% similarity) from the Ross Sea, Antarctica. A genetic study revealed two separate clades within B. sanguinolenta (
The specimen was found on the sedimented seafloor of an abyssal plain in APEI 1 at 5249 m depth.
No exactly similar Benthodytes sp. morphotypes have been so far catalogued from seabed imagery collected in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of specimen CCZ_178 was catalogued as a new morphotype (i.e., Benthodytes sanguinolenta sp. inc., HOL_124).
Clarion-Clipperton Zone • 1 specimen; APEI 7; 5.1043°N, 141.8865°W; 4861 m deep; 25 May. 2018; Smith & Durden leg.; GenBank: ON400682 (COI);
Single specimen (Fig.
The COI sequence is identical to the holotype of B. marianensis (K2P genetic distance = 0%) collected in the Mariana Trench at 5567 m depth (
The specimen was found on the sedimented seafloor of an abyssal plain in APEI 7 at 4860 m depth.
CCZ_019 resembles a Benthodytes sp. morphotype (i.e., Benthodytes sp. indet., HOL_111) encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ (
Family Elpidiidae Théel, 1882
Single specimen observed swimming (Fig.
The specimen collected during the DeepCCZ expedition was recovered in bits, so no morphological features can be distinguished. Only four reddish orange tentacles were recovered, which are embedded in a transparent skin where ossicles are evident. However, P. leander is one of the few species that can be identified from images. The external morphological characters evident in in situ images from the CCZ specimen are in accordance with the species description. The species was originally described from in situ images and video footage collected across the eastern CCZ (
In the phylogenetic tree, the CCZ specimen was recovered in a well-supported clade with other species of Peniagone (Fig.
The specimen was found swimming near the sediment surface on an abyssal plain in APEI 7 at 4860 m depth.
Peniagone leander (HOL_028) has been commonly encountered in seabed image surveys conducted across the eastern CCZ (e.g.,
Pacific Ocean • 1 specimen, syntype of Peniagone vitrea var. setosa Ludwig; South Pacific; 0.6°S, 86.7667°W; 2418 m deep; Albatross Expedition;
Single specimen. Body long, ~ 3× as long as wide (Fig.
Peniagone vitrea Théel, 1882. Specimen CCZ_077: A, B in situ images C lateral view before preservation D dorsal view E dorsal ossicles. Scale bars:2 cm (A); 3 cm (B); 200 μm (E). Image attribution: Durden and Smith (A, B); Wiklund, Durden, Drennan, and McQuaid (C, D); Bribiesca-Contreras (E)
Morphological external characters and ossicle morphology are in accordance with the original description of Peniagone vitrea. Unfortunately, no genetic sequences of P. vitrea are available in public databases. This species was described from off Patagonia at 2652 m depth. Using data from
The specimen was found feeding on the sedimented seafloor of an abyssal plain in APEI 7 at 4874 m.
A very similar Peniagone sp. morphotype (i.e., Peniagone vitrea sp. inc., HOL_059) has been commonly encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ (e.g.,
Family Laetmogonidae Ekman, 1926
Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8877°N, 141.757°W; 3132 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400690 (COI), ON406630 (18S);
Four specimens (Fig.
Psychronaetes sp. CCZ_101. Specimen CCZ_063 A in situ image. Specimen CCZ_101 B in situ image F dorsal ossicles. Specimen CCZ_103 C in situ image H dorsal ossicles. Specimen CCZ_104 D in situ image E dorsal view of specimen before preservation G ventral view. Scale bars: 5 cm (A, D); 2 cm (B); 1 cm (E, G); 75 μm (F); 100 μm (H). Image attribution: Durden and Smith (A–D); Wiklund, Durden, Drennan, and McQuaid (E, G); Bribiesca-Contreras (F, H).
Based on ossicle morphology, the four specimens were considered to belong to the same species. Sequence of the 18S were found to be identical between specimens CCZ_063, CCZ_101, and CCZ_104 (0.0% K2P distance) but 1.3% divergent from CCZ_103. The COI gene was amplified for the four specimens and genetic divergence ranges between 0.8% to 7.4%. The two specimens collected in APEI 4 are less genetically divergent (CCZ_101-CCZ_104 = 0.8% K2P). The specimen collected in APEI 7 (CCZ_063) is 2.3–2.9% divergent from the other three. The specimen CCZ_103 is 7.4% divergent to CCZ_101 and CCZ_104, but only 2.9% divergent from CCZ_063. While the former values are within the range of interspecific genetic divergence, we considered the specimen to belong to the same species as both the ossicle and external morphological characters are similar to the other three specimens. In addition, the trace files for both 18S and COI for this specimen are messy and the high genetic divergence could be an artifact of miss-called nucleotides. Unfortunately, there are no sequences available for Psychronaetes, but we included sequences of other genera within the family (Pannychia, Laetmogone, and Benthogone) for which COI genetic divergence ranged from 23–31%. In the phylogenetic tree, the four specimens were recovered in a well-supported clade (Fig.
The four specimens were found on the sedimented seafloor of seamounts in APEIs 4 and 7 between 3132–3562 m depth.
No exactly similar Psychronaetes sp. morphotypes have been encountered in seabed image surveys conducted in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ images of these specimens were catalogued as a new morphotype (i.e., Psychronaetes sp. indet., HOL_110). However, HOL_110 can resemble at least two other Laetmogonidae morphotypes catalogued from seabed imagery; Laetmogonidae gen. indet., HOL_030 (e.g., dark violet, but with 8+ long papillae) which is commonly found in the eastern CCZ (but not in the Kiribati EEZ); and Psychronaetes sp. indet., HOL_122 (e.g., violet, but only with six or seven long papillae and with fewer (< 20) and larger, thick tube feet) which was also only found in the western CCZ.
Pacific Ocean • 1 specimen, holotype of Laetmogone spongiosa Théel, 1879; south of Japan; 34.1167°N, 138°E; 1033 m deep; Challenger Expedition, Stn. 235;
Single specimen (Fig.
Laetmogone cf. wyvillethomsoni Théel, 1979. Specimen CCZ_062 A in situ image; B dorsal view of specimen before preservation C ventral view D dorsal calcareous ossicles. Scale bars: 2 cm (A); 1 cm (B, C); 50 μm (D). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B, C); Bribiesca-Contreras (D).
Closest match on public databases for the COI gene sequence was other sequences of Laetmogone wyvillethomsoni Théel, 1879 (4.0–5.8% K2P genetic distance) from the Ross Sea and Marie Byrd Seamounts (
The specimen was found on the sedimented seafloor of a seamount in APEI 7 at 3132 m depth.
No similar laetmogonid morphotypes have been encountered in seabed image surveys conducted in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of specimen CCZ_062 was catalogued as a new morphotype (i.e., Laetmogone sp. indet., HOL_123).
Class Ophiuroidea
To date, there are 1201 records of ophiuroids occurring at > 3000 m depth in the CCZ, with 117 representing preserved specimens (
Phylogenetic tree of Ophiuroidea. Concatenated (28S, and COI) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.
Subclass Myophiuroidea Matsumoto, 1915
Infraclass Metophiurida Matsumoto, 1913
Superorder Ophintegrida O’Hara, Hugall, Thuy, Stöhr & Martynov, 2017
Order Ophioscolecida O’Hara, Hugall, Thuy, Stöhr & Martynov, 2017
Family Ophioscolecidae Lütken, 1869
Single specimen (disc diameter = 9 mm, maximum arm length = 25 mm). Disc subpentagonal, flattened (Fig.
Ophiocymbium tanyae Martynov, 2010 A dorsal view of specimen CCZ_206 before preservation B ventral view C detail of dorsal disc surface and dorsal arm plates D detail of jaws, ventral disc surface and ventral arm plates. Scale bars: 2 cm (A, B); 5 mm (C, D). Image attribution: Wiklund, Durden, Drennan, and McQuaid (A–D).
Morphological characters of the specimen are in accordance with the description of O. tanyae, which was collected in the Izu-Bonin Trench at 6740–6850 m depth. It differs from the original description in having arms ≥ 2× as long as the disc diameter (dd), instead of being approx. the same. It also differs on the tentacle scales, which extend to the fifth segment, instead of just the third, having two tentacle scales in the first four segments instead of just one, and in the number of arm spines of the first arm segments. The number of arm spines is discussed to vary amongst the paratypes (
The specimen was found on the sedimented seafloor of an abyssal plain on APEI 1 at 5204 m depth.
Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2518°N, 153.6059°W; 5206 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON400727 (COI);
Single specimen, with white arms and greyish blue disc in situ (Fig.
Ophiocymbium cf. rarispinum Martynov, 2010. Specimen CCZ_197 A in situ image B dorsal surface before preservation C detail of ventral surface, jaws and ventral arm plates D detail of dorsal arm plates. Scale bars: 1 cm (A, B); 5 mm (C); 2.5 mm (D). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B–D).
In the phylogenetic tree, the specimen from the western CCZ is recovered as closely related to Ophiocymbium tanyae (Fig.
The specimen was found crawling on the abyssal sediments of APEI 1 at 5206 m depth.
A similar Ophiuroidea morphotype (i.e., Ophiocymbium sp. indet., OPH_013) has been encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ, but not in the abyssal areas surveyed within the Kiribati EEZ.
Superorder Euryophiurida O’Hara, Hugall, Thuy, Stöhr & Martynov, 2017
Order Ophiurida Müller & Troschel, 1840 sensu O’Hara et al. 2017
Suborder Ophiurina Müller & Troschel, 1840 sensu O’Hara et al. 2017
Family Ophiopyrgidae Perrier, 1893
Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.9081°N, 141.6813°W; 3239 m deep; 26 May. 2018; Smith & Durden leg.; GenBank: ON400685 (COI);
Two specimens, with greyish disc and pale arms in situ (Fig.
Ophiuroglypha cf. irrorata (Lyman, 1878). Specimen CCZ_059 A in situ image. Specimen CCZ_058 B in situ image C dorsal view of specimen before preservation D ventral view E arm hooklets F detail of ventral disc surface and ventral arm plates. Scale bars: 2 cm (A, B); 1 cm (C, D); 2 mm (F). Image attribution: Durden and Smith (A, B); Wiklund, Durden, Drennan, and McQuaid (C–F).
Dorsal arm plates fan-shaped, contiguous. Lateral arm plates bear three short (less than a third of the length of the arm segment) arm spines from the third arm segment; two are located ventrally, very close together, and one located dorsally, approx. halfway through the lateral arm plate; first arm segment bears two arm spines, the second two or three spines. Ventral arm plate trapezoidal, wider than long, only touching the preceding plate only on first three arm segments, after which they are separated by the lateral arm plates and become fan-shaped to rhomboidal, more than twice as wide as long, with pointed proximal edge and rounded distal margin. Towards the distal end of the arms, the second lowest spine is modified into a hyaline hooklet (Fig.
Both specimens collected are only 0.4% divergent (K2P distance) in COI sequences between them. Closest genetic match is Ophiuroglypha sp. (8% K2P distance) collected in the CCZ (
Both specimens were found on the sedimented seafloor of a seamount in APEI 7, at 3096 (specimen CCZ_059) and 3239 m (specimen CCZ_058) depth.
No similar Ophiuroidea morphotypes have been encountered in seabed image surveys conducted in the eastern CCZ nor in abyssal areas of the Kiribati EEZ. Consequently, the in situ images of CCZ_058 and CCZ_059 were catalogued as a new morphotype (i.e., Ophiuroglypha sp. indet., OPH_012).
Phylum Porifera Grant, 1836
A total of eight sponges was collected in the western CCZ. All these belong to the class Hexactinellida and represent seven different species, but none was confidently assigned to any known species. To date, there are 255 records of hexactinellid sponges occurring at > 3000 m depth in the CCZ, with only eight representing preserved specimens (
Phylogenetic tree of Hexactinellida. Concatenated (16S, 18S, 28S, and COI) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.
Class Hexactinellidae Schmidt, 1870
Subclass Amphidiscophora Schulze, 1886
Order Amphidiscosida Schrammen, 1924
Family Hyalonematidae Gray, 1857
Clarion-Clipperton Zone • 1 specimen; APEI 7; 5.1149°N, 141.8967°W; 4856 m deep; 25 May. 2018; Smith & Durden leg.; GenBank: ON400683 (COI), ON406634 (18S), ON406608 (16S), ON406597 (28S), ON411254 (ALG11);
Two specimens. Lophophytous sponges (Fig.
Genetic sequences between specimens CCZ_020 and CCZ_179 are 1% and 0.8% divergent (K2P distance) for COI and 16S, respectively. COI and 16S closest matches are sequences from Tabachnickia sp. within the Hyalonematidae. The sequence for the 18S is > 95% similar to other species of Hyalonema. In the phylogenetic tree, both specimens were recovered together, in a well-supported clade with other members from different subgenera within the genus Hyalonema and including Tabachnickia sp. (Fig.
The specimens were collected attached to abyssal sediments of APEI 7 and APEI 1 at 4856 and 5245 m depth, respectively.
A very similar hyalonematid morphotype (i.e., Hyalonema sp. indet., HEX_002) has been commonly encountered in seabed image surveys conducted across the eastern CCZ and in abyssal areas of the Kiribati EEZ, mostly in nodule field areas. In situ images of HEX_002 (Fig.
Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.036°N, 149.9395°W; 5031 m deep; 01 Jun. 2018; Smith & Durden leg.; GenBank: ON406610 (16S);
Single specimen (Fig.
Morphological characters were found concordant with those of the genus Hyalonema. The 16S sequence is very similar (99.34%) to sequences from H. (Cyliconemaoida) ovuliferum Schulze, 1899, being the closest match on public databases. It is recovered in a well-supported clade along with other hyalonematids (Fig.
This specimen was collected anchored to the sediment on the abyssal plain of APEI 4 at 5031 m.
A very similar hyalonematid morphotype (i.e., Hyalonema sp indet., HEX_003) has been commonly encountered in seabed image surveys conducted across the eastern CCZ and in abyssal areas of the Kiribati EEZ. As observed in HEX_002, the aperture width of the central osculum in HEX_003 can vary owing to body contractions or expansions (
Subclass Hexasterophora Schulze, 1886
Order Lyssacinosida Zittel, 1877
Family Euplectellidae Gray, 1867
Subfamily Euplectellinae Gray, 1867
Euplectellinae stet. CCZ_199
Fig.
Material. Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2518°N, 153.5853°W; 5202 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON400729 (COI), ON406611 (16S);
Description. Single specimen (Fig.
Remarks. The 16S sequence is close to Corbitella discasterosa Tabachnick & Lévi, 2004 (2.3% K2P distance), and it is also similar to other species within the family Euplectellidae. The closest COI match is Docosaccus maculatus Kahn, Geller, Reiswig & Smith Jr., 2013 (91.5% similarity). Morphological characters are concordant with those of the family Euplectellidae, and in the phylogenetic analysis it is recovered within the Euplectellidae (Fig.
Ecology. This specimen was found anchored to the abyssal sediments of APEI 1 at 5202 m depth.
Comparison with image-based catalogue. A very similar Euplectellidae morphotype (i.e., Euplectellidae gen. indet., HEX_005) has been commonly encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ, but not in abyssal areas of the Kiribati EEZ.
Single specimen; lophophytous sponge. Plate-like, flat, subcircular body; 8.7 cm at its longest axis, 1 mm thick (Fig.
External morphological characters are concordant with the description of D. maculatus (
This specimen was found anchored to abyssal sediments of APEI 7 at 4860 m depth.
A very similar Docosaccus sp. morphotype (i.e., Docosaccus maculatus sp. inc., HEX_015) has been very frequently encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ and in abyssal areas of the Kiribati EEZ.
Single specimen; lophophytous white sponge (Fig.
Morphological external characters are concordant with the description of Holascus spinosus Kersken, Janussen & Martínez Arbizu, 2019, which was described from the IOM area in the CCZ. The closest genetic matches on GenBank for the 16S correspond to species within the genus Holascus (2.1–3.5% genetic divergence), with the holotype of Holascus spinosus being the closest match (2.1% genetic divergence). There are no 18S sequences available for H. spinosus, but it is 0.23% divergent from another species within the genus, H. euonyx (Lendenfeld, 1915), from which it differs morphologically. In the phylogenetic tree it was recovered, with low support, as sister to H. spinosus (Fig.
This specimen was found anchored to abyssal sediments of APEI 7 at 4874 m depth.
A very similar Holascus sp. morphotype (i.e., Holascus sp. indet., HEX_014) has been commonly encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ and in abyssal areas of the Kiribati EEZ.
Subfamily Bolosominae Tabachnick, 2002
Bolosominae stet. CCZ_198
Fig.
Material. Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2518°N, 153.6053°W; 5205 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON400728 (COI), ON406637 (18S), ON406614 (16S), ON406600 (28S);
Description. Single specimen; lophophytous white sponge (Fig.
Remarks. The closest match with the 18S sequence is the holotype of Hyalostylus microfloricomus Kersken, Janussen & Martínez Arbizu, 2019 (99.8%), described from the Heip Mountains in the GSR contract area in the CCZ at 3788 m depth (
Ecology. This specimen was collected on abyssal sediments of APEI 1 at 5205 m depth, and was anchored to the sediment.
Comparison with image-based catalogue. A very similar stalked sponge morphotype (i.e., Hexactinellidae ord. indet., HEX_026) has been encountered in seabed image surveys conducted at the eastern CCZ, but not in abyssal areas of Kiribati’s EEZ. In seabed images, HEX_026 highly resembles Hyalonema (Cyliconemaoida) campanula Lendenfeld, 1915, as identified by
Order Sceptrulophora Mehl, 1992
Family Euretidae Zittel, 1877
Subfamily Chonelasmatinae Schrammen, 1912
Single specimen; basiphytous sponge (Fig.
Morphological characters are concordant with those of the genus, being very similar to Bathyxiphus subtilis Schulze, 1899, the only known species in the genus. However, a midrib has been suggested as a key morphological feature absent in the specimen presented here. The species was described from Isla Guadalupe at 1251 m depth, and was recently recorded in APEI 3 at 4914 m (
The specimen was found attached to a beaked-whale rostrum covered in polymetallic crust, on abyssal sediments of APEI 4 at 5001 m depth.
A similar Bathyxiphus sp. morphotype (i.e., Bathyxiphus sp. indet., HEX_025), though usually much smaller-sized, has been commonly encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ, but not in abyssal areas of the Kiribati EEZ.
The DeepCCZ expedition surveyed three APEIs on the western CCZ, targeting both abyssal seafloor and seamounts, and sampling the different megafaunal components. The ROV survey for benthic megafauna yielded a remarkably diverse collection from a small number of specimens, with 48 species from only 55 specimens (Table
Many of the taxa presented here had been encountered in image-based surveys from across the CCZ but never collected before, making our collections particularly important for improving taxonomic knowledge (
Although the CCZ is often considered a vast and relatively homogeneous abyssal plain, this region has substantial, ecologically important, seafloor heterogeneity (
While the lack of shared species between habitats in this study could be a result of undersampling, faunistic changes between habitats have been reported in the CCZ (
The study of deep-sea ecosystems presents several challenges, from the difficulties of collecting at great depths, to the resources required for an oceanographic survey, and the labour to document and describe all the collected material (
One of the limitations of this study is the scarcity of published barcodes for deep-see invertebrates. For instance, an environmental DNA study in the western APEIs found that only 25% and 1.5% of OTUs could be assigned to family level using reference libraries for 18S and COI, respectively (
Additional challenges result from the documentation that COI and other mitochondrial genes show very little variation in anthozoan cnidarians (
While detailed taxonomic studies can reveal an overlooked biodiversity in deep-sea taxa and are greatly improving our understanding of species ranges, they are time-consuming and usually target a small area or a few taxa (e.g.,
The alignment of in situ specimen images from this study with invertebrate morphotypes previously catalogued from (and standardised across) different seabed image surveys conducted in the CCZ (
We provide the first megafaunal faunistic study from the western CCZ based on voucher specimens. Our findings indicate a high diversity, represented mostly by undescribed species of megafauna in the western CCZ with little overlap between abyssal plains and seamounts, and within similar habitats located in greater distances to one another. Further studies should aim to increase our knowledge of patterns of biodiversity across the entire CCZ in order to inform environmental management plans to protect its biodiversity. Our work also highlights the need for detailed taxonomic studies, not only within the CCZ, an area targeted for deep-sea mining, but in other bathyal, abyssal, and hadal regions. While species identification through genetic markers can facilitate the generation of species inventories, this is only achievable when genetic reference libraries are representative of the area and taxon of study, and these remains limited for the CCZ megafauna.
We want to acknowledge the masters, crew and technical support staff on the R/V Kilo Moana and ROV Lu’ukai during the DeepCCZ expedition. We are very grateful for help with taxonomic identifications provided by the expert taxonomists: Estefania Rodriguez (AMNH) for anemones, Dhugal Lindsay (JAMSTEC) for scyphozoans; Chris Mah (NMNH) for sea stars; Rich Mooi (CAS) and Carlos Andres Conejeros Vargas (UNAM) for sea urchins; and Lenka Nealova (NHM) for annelids. We also acknowledge Lauren Hughes (NHM), Miranda Lowe (NHM), Tom White (NHM), Amanda Robinson (NMNH), Emma Sherlock (NHM), Andreia Salvador (NHM), and Andrew Cabrinovic (NHM) for curatorial support; and Elena Luigli (NHM) and Claire Griffin (NHM) for lab support. Primary funding was from the Gordon and Betty Moore Foundation grant no. 5596 and NOAA Office of Ocean Exploration (grant #NA17OAR0110209), and the University of Hawaii. We also acknowledge funding from UK Seabed Resources, the UK Natural Environment Research Council grant numbers NE/T003537/1 and NE/T002913/1 and through National Capability funding to NOC as part of the Climate Linked Atlantic Section Science (CLASS) programme (grant number NE/R015953/1), and The Norwegian Research Council (JPIOMining Impact 2). DJA received funding from the EU’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement number 747946.
Table S1
Data type: phylogenetic
Explanation note: Detail of sequences included in the phylogenetic analyses including details of voucher numbers, code, GenBank accession number for different genes, details on whether specimens included are part of type material.