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Benthic megafauna of the western Clarion-Clipperton Zone, Pacific Ocean
expand article infoGuadalupe Bribiesca-Contreras, Thomas G. Dahlgren§|, Diva J. Amon, Stephen Cairns#, Regan Drennan¤, Jennifer M. Durden«, Marc P. Eléaume», Andrew M. Hosie˄, Antonina Kremenetskaia˅, Kirsty McQuaid¦, Timothy D. O’Haraˀ, Muriel Rabone¤, Erik Simon-Lledó«, Craig R. Smithˁ, Les Watlingˁ, Helena Wiklund§, Adrian G. Glover¤
‡  Life Sciences Department, Natural History Museum, London, United Kingdom
§ University of Gothenburg, Gothenburg, Sweden
| Norwegian Research Centre, NORCE, Bergen, Norway
¶ SpeSeas, D’Abadie, Trinidad and Tobago
# National Museum of Natural History, Smithsonian Institution, Washington, United States of America
¤ Life Sciences Department, Natural History Museum, London, United Kingdom
« National Oceanography Centre, Southampton, United Kingdom
» Départment Origines et Évolution, Muséum national d’Histoire Naturelle, Paris, France
˄ Collections & Research, Western Australia Museum, Perth, Australia
˅ Shirshov Institute of Oceanology, Russian Academy of Sciences, Moscow, Russia
¦ University of Plymouth, Plymouth, United Kingdom
ˀ Museums Victoria, Melbourne, Australia
ˁ University of Hawai’i at Mānoa, Honolulu, United States of America
Open Access

Abstract

There is a growing interest in the exploitation of deep-sea mineral deposits, particularly on the abyssal seafloor of the central Pacific Clarion-Clipperton Zone (CCZ), which is rich in polymetallic nodules. In order to effectively manage potential exploitation activities, a thorough understanding of the biodiversity, community structure, species ranges, connectivity, and ecosystem functions across a range of scales is needed. The benthic megafauna plays an important role in the functioning of deep-sea ecosystems and represents an important component of the biodiversity. While megafaunal surveys using video and still images have provided insight into CCZ biodiversity, the collection of faunal samples is needed to confirm species identifications to accurately estimate species richness and species ranges, but faunal collections are very rarely carried out. Using a Remotely Operated Vehicle, 55 specimens of benthic megafauna were collected from seamounts and abyssal plains in three Areas of Particular Environmental Interest (APEI 1, APEI 4, and APEI 7) at 3100–5100 m depth in the western CCZ. Using both morphological and molecular evidence, 48 different morphotypes belonging to five phyla were found, only nine referrable to known species, and 39 species potentially new to science. This work highlights the need for detailed taxonomic studies incorporating genetic data, not only within the CCZ, but in other bathyal, abyssal, and hadal regions, as representative genetic reference libraries that could facilitate the generation of species inventories.

Keywords

Biogeography, deep-sea mining, DNA barcoding, DNA taxonomy, megafauna, polymetallic nodules

Introduction

The Clarion-Clipperton Zone (CCZ) in the central abyssal Pacific has become of great interest for deep-sea mineral extraction. This large area of abyssal seafloor, approximately 6 million km2 (Wedding et al. 2013), has the largest concentrations of high-grade polymetallic nodules, representing a vast source of commercially valuable metals such as nickel, copper, and cobalt, many of which are currently used in high-tech and green industries (Hein et al. 2020). Although new technological advances are taking deep-sea mining closer to reality, the impacts of mining activities on deep-sea ecosystems remain of concern and are still poorly understood (Jones et al. 2017). To date, the International Seabed Authority (ISA), which governs seabed mining in this area, has granted 17 exploration contracts to permit baseline surveys and resource assessment (but not commercial mining) in the CCZ, and has adopted an environmental management plan establishing 13 areas where exploitation is currently prohibited (called Areas of Particular Environmental Interest, or APEIs) (Smith et al. 2021). Four of these were recently implemented, but the representativity of the APEI network still needs to be assessed.

During the last few decades, there has been a dramatic increase in the scientific exploration of the CCZ, but our knowledge of the faunal communities associated with nodule fields is still limited, and taxonomic records for the area are scarce (Glover et al. 2018). Although the CCZ was first explored in 1875 by the H.M.S. Challenger (Thomson and Murray 1885), relatively little taxonomic work has been carried out in this vast area and hence very little biogeographic information is available (Simon-Lledó et al. 2020). This is particularly problematic as such information is critical to characterise the biodiversity, biogeographic ranges, and connectivity patterns across the entire CCZ in order to make better predictions about the potential impacts of deep-sea mining. In addition, the APEIs designated to preserve regional biodiversity are severely understudied (Glover et al. 2016a; International Seabed Authority 2020; Jones et al. 2021).

The CCZ abyssal seafloor is rich in topographic features such as hills, troughs, fracture zones, and seamounts (Kaiser et al. 2017). It encompasses many habitats with a range of different environmental conditions such as depth, nodule coverage, sediment composition, bathymetric relief, flow intensification on seamounts, and particulate organic carbon (POC) flux (Wedding et al. 2013; International Seabed Authority 2020; McQuaid et al. 2020; Washburn et al. 2021b). Benthic assemblages have been found to change across the CCZ (Wilson 2017; Bonifácio et al. 2020; Simon-Lledó et al. 2020), with POC flux influencing regional megafaunal community patterns, and local environmental factors (i.e., nodule coverage) and bathymetric features having an effect at local scales (Amon et al. 2016; Simon-Lledó et al. 2019c). Seamounts are abundant in the CCZ, most commonly in the eastern and western ends of the area, with elevations of > 1000 m above the plain, and are a major source of hard-substrate habitat (Wedding et al. 2013). Even though seamounts were hypothesised to provide a potential refugia and to be larval sources of nodule-associated fauna that could aid in recolonising nodule fields, of similar depths, disturbed by mining activities, a recent study suggested that the seamounts sampled in the CCZ appear inadequate as refuge areas (Cuvelier et al. 2020). Nonetheless, the biodiversity of the CCZ seamounts remains largely unknown, with only few having been explored on the eastern (Cuvelier et al. 2020; Jones et al. 2021) and western (Durden et al. 2021) margins.

Large benthic organisms (benthic megafauna) have been prioritised for monitoring deep-sea ecosystems because they can be studied from seabed imagery (Danovaro et al. 2020), provide inferences on trophic interactions, ecosystem functioning (Rex and Etter 2010), and processes of disturbances (Jones et al. 2017) and recovery (Simon-Lledó et al. 2019a). In the CCZ, megafaunal benthic assemblages have been studied almost exclusively from video and still images (e.g., Amon et al. 2016; Simon-Lledó et al. 2019c, 2020; Cuvelier et al. 2020; Durden et al. 2021). While these studies have vastly increased our understanding of biodiversity and community structure, uncertainty remains as to the identity of operational taxonomic units (unique identifiers for different morphospecies) recognised in imaged-based survey, and whether they are conspecific with other known species from elsewhere in the deep sea. It is thus critical to complement spatial/temporal analyses with detailed morphological and DNA-sequence analyses of collected specimens (Amon et al. 2016, 2017b).

The DeepCCZ project was conceived to increase our understanding of faunal assemblages and biodiversity in the western CCZ, targeting both unexplored seamounts and APEIs. Here, we provide the first taxonomic synthesis of western CCZ megafauna, which is also the largest megafaunal faunistic study from anywhere in the CCZ based on collected specimens. We provide morphological descriptions, genetic data, and high-resolution imagery for all megafauna specimens collected, including specimens from both the abyssal plains and seamount habitats. It complements similar studies of the high diversity of megafaunal xenophyophores (Gooday et al. 2020a, b), and imagery-based community analysis of the megafauna in this area (Durden et al. 2021).

Materials and methods

The DeepCCZ expedition, aboard the RV Kilo Moana, from 14 May to 16 June 2018, surveyed seamounts and abyssal plains in three Areas of Particular Environmental Interest (APEIs 1, 4, and 7) located in the western Clarion-Clipperton Zone (CCZ; Fig. 1). All material presented here was collected during this expedition using the Remotely Operated Vehicle (ROV) Lu’ukai, and specimens were processed following the DNA taxonomy pipeline described in Glover et al. (2015).

Figure 1. 

Map of the Clarion-Clipperton Zone (top left) indicating the nine Areas of Particular Environmental Interest (APEIs) in red, exploration areas in green, and reserved areas in orange. Shapefiles were sourced from https://www.naturalearthdata.com/downloads/10m-physical-vectors/10m-bathymetry/, and https://www.isa.org.jm/minerals/maps. Detailed maps of the study areas: APEIs 1 (top right), 4 (bottom left), and 7 (bottom right) show bathymetry from satellite values for the entire APEI, and multibeam values obtained during the DeepCCZ expedition. Sites, and specific geoform, where megafauna samples were collected are indicated as yellow stars in abyssal plains, green triangles in seamounts, and pink hexagons in seamount slopes.

Data resources

Sequences generated for this study have been deposited on GenBank: ON400681ON400730 (COI), ON406602ON406622 (16S), ON406623ON406643 (18S), ON406596ON406601 (28S), and ON411254ON411256 (ALG11).

Sampling

Specimens were selected from across as many taxonomic groups as possible, with duplicates of similar morphotypes avoided; thus, the aim was to increase our understanding of megafaunal diversity. A total of 55 specimens were collected during different dives in three APEIs and three different geoforms (abyssal plain, seamount, and seamount slope), 14 specimens were collected from abyssal seafloor in APEI 1; 13 from abyssal seafloor, two from the seamount slope and six from a seamount in APEI 4; and nine from abyssal seafloor and 11 from a seamount in APEI 7 (Fig. 1). In situ photographs and/or video frame grabs of each specimen were captured, with parallel lasers for scale at 15 cm spacing. Megafaunal specimens were collected with the manipulator (‘Orion’), suction sampler, or push cores, depending on the characteristics of each specimen in order to best preserve morphological characters. Specimens collected with the manipulator were placed into the biobox receptacle, while those collected with the suction sampler were stored in a suction box. Collection data were recorded at the time of capture (e.g., date and time, ROV latitude/longitude, seabed water depth, ROV waypoint name).

After ROV recovery, specimens were transferred and maintained in cold (2–4 °C), filtered seawater until processed. Following Glover et al. (2015), all specimens were photographed, given a preliminary identification and assigned a unique voucher code (e.g., CCZ_020). A tissue sample was taken from each specimen for downstream molecular analyses, and stored in 95% non-denatured ethanol at -20 °C. Specimens were fixed in 10% borax-buffered formalin and transferred to 70% non-denatured ethanol after 48 h, except for sponges, which were kept frozen at -80 °C. After the expedition, specimens and tissue sub-samples were sent to the University of Hawai’i Manoa. Specimens were archived at the Natural History Museum, London (NHMUK), and the Western Australian Museum (WAM), following taxonomic inspection.

DNA extraction, amplification, and sequencing

DNA extraction was performed using the DNeasy Blood and Tissue Kit (Qiagen). The barcode gene cytochrome oxidase I (COI) was the main target because this gene has been used in previous studies on megafauna in the CCZ (e.g., Dahlgren et al. 2016; Glover et al. 2016b). Additional markers for specific taxa (16S, 18S, 28S, and ALG11) were amplified when either COI amplification was unsuccessful or to improve identification or phylogenetic inference. The PCR mix for each reaction contained 10.5 µl of Red Taq DNA Polymerase 1.1X MasterMix (VWR), 0.5 µl of each primer (10 µM), and 1 µl of DNA template. PCR protocols and primers used for COI, 16S, and 18S were following Glover et al. (2016b) and Dahlgren et al. (2016), and Hestetun et al. (2016) for ALG11 and 28S. The PCR products were purified and sequenced at the NHM Sequencing Facilities using a Millipore Multiscreen 96-well PCR Purification System and ABI 3730XL DNA Analyser (Applied Biosystems), respectively. Sequencing primers used were the same as those for the PCR reactions, with the addition of internal primers for 18S and only using primers from the second PCR for the ALG11 gene. DNA sequences were analysed using Geneious 7.0.6 (https://www.geneious.com), with contigs assembled from both forward and reverse sequences and ambiguous base calls corrected manually. DNA sequences generated in this study were submitted to GenBank, with accession numbers ON400681ON400730 for COI, ON406602ON406622 for 16S, ON406623ON406643 for 18S, ON406596ON406601 for 28S, and ON411254ON411256 for ALG11.

Phylogenetic assignments

Phylogenetic relationships of the western CCZ megafauna were explored by estimating phylogenetic trees for all taxa at different taxonomic levels: phylum Annelida: family Aphroditidae; phylum Arthropoda: order Scalpellomorpha; phylum Cnidaria: order Actiniaria, subclass Ceriantharia, subclass Octocorallia, and class Scyphozoa; phylum Echinodermata: class Asteroidea, class Crinoidea, class Echinoidea, class Holothuroidea, and class Ophiuroidea; and phylum Porifera. Different sets of genes were used depending on published phylogenies and publicly available sequences for each taxon, considering both nuclear and mitochondrial genes if available. For each taxon, sequences were obtained from GenBank (Suppl. material 1: Table S1), except for Porifera as a published alignment was used (Dohrmann 2018). Protein-coding genes (COI, cytochrome oxidase III: COX3, mtMutS homolog: msh1, NADH-dehydrogenase subunit 2 gene: ND2) were aligned using MUSCLE in MEGA-X. Non-protein-coding genes (12S, 16S, 18S, 28S) were aligned using MAFFT v. 7 (Katoh et al. 2019) using the auto strategy, and unalignable regions filtered in GBLOCKS (Castresana 2000), allowing gap positions within final blocks and less strict flanking positions. Individual gene-alignments were concatenated in Geneious, and the best substitution model for each partition was determined using PartitionFinder 2 (Lanfear et al. 2017). For Porifera, we manually aligned our sequences for the 18S, 28S, 16S, and COI genes with the alignment provided in Dohrmann (2018).

Phylogenetic trees were estimated using partitioned maximum-likelihood (RAxML v8.2.10; Stamatakis 2006) and Bayesian inference (BEAST v. 2.4.7; Bouckaert et al. 2014), with the best inferred substitution model for each partition. In RAxML, the most common substitution model for each taxon was selected. RNA secondary structure, as in Dohrmann (2018), was also considered for Porifera, using the S16+G substitution model to paired sites of 18S and 28S. BEAST analyses were performed with trees and clock models linked, a Yule tree model, and relaxed clock log normal. Two independent runs of a maximum 100 M steps were combined after discarding 20% as burn-in. Runs were checked for convergence and a median consensus tree was estimated from the combined post-burn-in samples.

Taxonomic assignments

Taxonomic assignments considered information drawn from both molecular and morphological analyses. For the latter, the collected specimens were sent to expert taxonomists for morphological assignments. We assigned every specimen to the lowest Operational Taxonomic Unit (OTU), each representing a species. However, we took a precautionary approach when assigning species names (Dahlgren et al. 2016; Glover et al. 2016b; Horton et al. 2021), therefore recording species as ‘cf.’ when uncertain about their identity based on (i) differences in morphological characters, (ii) missing type locality DNA data, or (iii) when type localities are at significantly different depths or vast distances from the western CCZ. Also, those species that could not be identified as a described species were given a unique identifier using the lowest taxonomic level confidently identified and the voucher code (assigned at sea; i.e., CCZ_060). In cases where more than one specimen represented a species with a unique identifier, this only included the voucher code of the best-preserved voucher specimen of that species. Additionally, open taxonomic nomenclature signs were used to indicate that the specimen was not identified any further (‘stet’; e.g., ‘Laetmonice stet. CCZ_060’), or when the identification is still uncertain (‘inc’; e.g., Bathymetrinae inc. CCZ_176), and ‘sp.’ was only used for potentially new species (e.g., Psychronaetes sp. CCZ_101) after Glover et al. (2016b).

Current records available on OBIS, at a minimum depth of 3000 m, were recovered for each taxon on January 12, 2022 (robis::occurrence; Provoost and Bosch 2020). Records within a box defined by 13°N, 158°W; 18°N, 118°W; 10°N, 112°W; 2°N, 155°W were considered as occurring within the CCZ (Glover et al. 2015).

Comparison with seabed-imagery database

To gain preliminary insight into connectivity and distributions, morphology of specimens was compared to and, where possible, aligned with a standardised megafauna morphotype catalogue developed from in situ seabed imagery from across the north Pacific abyss, mostly eastern CCZ (Simon-Lledó et al., pers. obs.). The catalogue aligns invertebrate morphotypes, only for specimens larger than 1 cm, encountered in quantitative megafaunal assessments. At the time of writing, the survey areas so far encompassed in the standardised megafauna catalogue are, from east to west: UK-1 (Amon et al. 2016); BGR, GSR, and APEI 3 (Cuvelier et al. 2020); APEI 6 (Simon-Lledó et al. 2019b); TOML areas B, C, and D (Simon-Lledó et al. 2020); APEIs 1, 4, and 7 (this study; Durden et al. 2021); and the EEZ of Kiribati (Simon-Lledó et al. 2019d). The catalogue assigns each documented taxon a 7-character, unique morphotype code (e.g., POR_001) that differs from unique identifiers used for the species in this study. The level of taxonomic precision achieved in each catalogued taxon is indicated using the open taxonomic nomenclature signs recommended for image-based identifications by Horton et al. (2021). A suffix is added to each morphotype identification specifying the taxonomic rank (e.g., "fam.", "family"; "gen.", "genus" or "sp.", "species") and the signs "indet."or "inc.". The "indet." (indeterminabilis) indicates that further identification was not possible as diagnostic features are not typically visible in images, while the "inc." (incerta) indicates that despite diagnostic features being visible in images, the identification still has some uncertainty, needing further comparable material for validation.

Results

A total of 55 specimens was collected in the western CCZ (Table 1). Based on molecular data these represent 48 species of invertebrate megafauna (43 singletons, four doubletons, and a single species with four representatives) belonging to ten classes in five phyla. However, for three of the doubletons, each specimen was collected in a different APEI, but all were consistently found in the same geoform (i.e., abyssal plain, seamount, or seamount slope). Most of the taxa were collected on the abyssal seafloor (36 specimens from 33 species) > 4800 m deep, followed by seamounts (17 specimens from 13 species) between 3095–3562 m deep, and only two specimens from two species collected on a seamount slope at 4125 m deep. Out of the 48 taxa, only nine were assigned to previously described species, all from adjacent regions such as the Kuril-Kamchatka, Mariana, and Izu-Bonin Trenches, the South China Sea, and other areas of the Northwest and Southwest Pacific.

Table 1.

Megafauna specimens collected during the DeepCCZ expedition, including details of their collection such as collection site and geoform (S, seamount; AP, abyssal plain; Sl, seamount slope), substrate or attachment (S, on sediment; E, epibiont, N, nodule; C, crust, Sa, anchored to sediment; B, attached to bone), depth, decimal latitude and longitude, scientific collection and accession number, voucher number, and GenBank accession number.

Classification Species Site Substrate / Attachment Depth (m) Coordinates (Latitude, Longitude) Collection Accession no. Voucher GenBank accession no.
Annelida Polychaeta Phyllodocida Aphroditidae Laetmonice stet. CCZ_060 APEI 7 (S) S 3096 4.8897, -141.7500 NHMUK 2022.760 CCZ_060 ON400687 (COI)
Arthropoda Thecostraca Scalpellomorpha Scalpellidae Trianguloscalpellum gigas APEI 7 (AP) E 4875 5.0442, -141.8165 WAM C74110 CCZ_074 ON400698 (COI), ON406624 (18S)
Catherinum cf. albatrossianum APEI 7 (AP) E 4875 5.0442, -141.8165 WAM C74109 CCZ_073 ON400697 (COI), ON406623 (18S)
Catherinum cf. novaezelandiae APEI 1 (AP) E 5241 11.2751, -153.7444 WAM C74111 CCZ_185 ON400722 (COI), ON406625 (18S)
Cnidaria Anthozoa Actiniaria Metridioidea stet. CCZ_072 APEI 1 (AP) E 4875 5.0442, -141.8165 NHMUK 2021.19 CCZ_072 ON400696 (COI)
Metridioidea stet. CCZ_154 APEI 4 (AP) N 5009 6.9702, -149.9426 NHMUK 2021.27 CCZ_154 ON400715 (COI)
Metridioidea stet. CCZ_164 APEI 7 (AP) E 5001 6.9880, -149.9326 NHMUK 2021.5 CCZ_164 ON400717 (COI)
Actinostolidae Actinostolidae stet. CCZ_183 APEI 1 (AP) N 5241 11.2751, -153.7444 NHMUK 2021.28 CCZ_183 ON406626 (18S)
Actinostolidae stet. CCZ_202 APEI 4 (AP) N 5206 11.2518, -153.6059 NHMUK 2021.22 CCZ_202 ON406627 (18S)
Scleractinia Caryophyllidae Fungiacyathus (Fungiacyathus) cf. fragilis APEI 4 (S) S 3562 7.2647, -149.7740 NHMUK 2021.26 CCZ_107 NA
Alcyonacea Chrysogorgiidae Chrysogorgia sp. CCZ_112 APEI 4 (Sl) C 4125 7.2874, -149.8578 NHMUK* CCZ_112 ON400711 (COI), ON406602 (16S)
Mopseidae Mopseidae sp. CCZ_088 APEI 4 (AP) N 5018 7.0089, -149.9109 NHMUK* CCZ_088 ON400705 (COI), ON406603 (16S)
Primnoidae Calyptrophora distolos APEI 4 (Sl) C 4125 7.2874, -149.8578 USNM 1550968 CCZ_113 ON400712 (COI), ON406604 (16S)
Pennatulacea Protoptilidae Protoptilum stet. CCZ_068 APEI 7 (S) Sa 3096 4.8897, -141.7500 NHMUK 2021.24 CCZ_068 ON400694 (COI), ON406605 (16S)
Spirularia Spirularia stet. CCZ_067 APEI 7 (S) Sa 3132 4.8875, -141.7572 NHMUK 2021.23 CCZ_067 ON400693 (COI), ON406606 (16S)
Scyphozoa Somaeostomeae Ulmaridae Ulmaridae stet. CCZ_069 APEI 7 (S) S 3133 4.8876, -141.7572 NHMUK 2021.25 CCZ_069 ON400695 (COI)
Echinodermata Asteroidea Brisingida Freyellidae Freyastera cf. tuberculata APEI 4 (AP) S 5000 6.9879, -149.9123 NHMUK 2022.79 CCZ_087 ON400704 (COI)
APEI 4 (AP) S 5000 6.9873, -149.9331 NHMUK 2022.80 CCZ_157 ON400716 (COI)
Freyastera stet. CCZ_201 APEI 1 (AP) S 5204 11.2518, -153.6059 NHMUK 2022.81 CCZ_201 ON400730 (COI)
Forcipulatida Zoroasteridae Zoroaster stet. CCZ_065 APEI 7 (S) S 3132 4.8877, -141.7569 NHMUK 2022.78 CCZ_065 ON400691 (COI), ON406607 (16S)
Crinoidea Comatulida Phrynocrinidae cf. Porphyrocrinus sp. CCZ_165 APEI 4 (AP) N 5002 6.9879, -149.9327 NHMUK 2022.76 CCZ_165 ON400718 (COI), ON406616 (16S)
Antedonidae Bathymetrinae incert. CCZ_176 APEI 4 (AP) E 5009 6.9879, -149.9326 NHMUK 2022.77 CCZ_176 ON400719 (COI), ON406617 (16S);
APEI 1 (AP) E 5241 11.2751, -153.7444 NHMUK 2022.60 CCZ_186 ON400723 (COI), ON406618 (16S)
Echinoidea Aspidodiadematoida Aspidodiadematidae Plesiodiadema cf. globulosum APEI 1 (AP) S 5204 11.2527, -153.5848 CASIZ 229305 CCZ_196 ON400726 (COI), ON406628 (18S)
Echinothurioida Kamptosomatidae Kamptosoma abyssale APEI 4 (AP) S 5040 7.0360, -149.9395 CASIZ 229306 CCZ_082 ON400701 (COI)
Holothuroidea Persiculida Molpadiodemidae Molpadiodemas stet. CCZ_102 APEI 4 (S) S 3552 7.2701, -149.7827 NHMUK 2022.66 CCZ_102 ON400708 (COI)
Molpadiodemas stet. CCZ_194 APEI 1 (AP) S 5205 11.2517, -153.6055 NHMUK 2022.71 CCZ_194 ON400725 (COI)
Synallactida Synallactidae Synallactes stet. CCZ_153 APEI 4 (AP) S 5009 6.9704, -149.9426 NHMUK 2022.69 CCZ_153 ON400714 (COI)
Synallactidae stet. CCZ_061 APEI 7 (S) S 3132 4.8877, -141.7569 NHMUK 2022.75 CCZ_061 ON400688 (COI), ON406640 (18S)
Synallactidae stet. CCZ_066 APEI 7 (S) S 3095 4.8896, -141.7500 NHMUK 2022.63 CCZ_066 ON400692 (COI), ON406642 (18S)
Deimatidae Oneirophanta stet. CCZ_100 APEI 4 (S) S 3550 7.2647, -149.7740 NHMUK 2022.84 CCZ_100 ON400706 (COI), ON406643 (16S), ON406620 (18S)
Oneirophanta cf. mutabilis APEI 1 (AP) S 5203 11.2520, -153.5847 NHMUK 2021.20 CCZ_193 ON400724 (COI), ON406629 (16S), ON406619 (18S)
Elasipodida Psychropotidae Psychropotes verrucicaudatus APEI 4 (AP) S 4999 6.9878, -149.9119 NHMUK 2021.19 CCZ_086 ON400703 (COI)
Psychropotes dyscrita APEI 4 (AP) S 5040 7.0212, -149.9355 NHMUK 2022.83 CCZ_083 ON400702 (COI)
Benthodytes cf. sanguinolenta APEI 1 (AP) S 5245 11.2953, -153.7420 NHMUK 2022.70 CCZ_178 ON400720 (COI)
Elasipodida Psychropotidae Benthodytes marianensis APEI 7 (AP) S 4861 5.1043, -141.8865 NHMUK 2022.82 CCZ_019 ON400682 (COI)
Elpidiidae Peniagone leander APEI 7 (AP) S 4860 5.1042, -141.8861 NHMUK 2022.61 CCZ_018 ON400681 (COI), ON406621 (16S)
Peniagone vitrea APEI 7 (AP) S 4875 5.0442, -141.8164 NHMUK 2022.64 CCZ_077 ON400699 (COI), ON406622 (16S)
Laetmogonidae Psychronaetes sp. CCZ_101 APEI 4 (S) S 3562 7.2647, -149.7741 NHMUK 2022.65 CCZ_101 ON400707 (COI), ON406631 (18S)
APEI 4 (S) S 3562 7.2647, -149.7741 NHMUK 2022.68 CCZ_104 ON400710 (COI), ON406632 (18S)
APEI 7 (S) S 3132 4.8877, -141.7570 NHMUK 2022.62 CCZ_063 ON400690 (COI), ON406630 (18S)
APEI 4 (S) S 3562 7.2647, -149.7741 NHMUK 2022.67 CCZ_103 ON400709 (COI), ON406639 (18S)
Laetmogone cf. wyvillethomsoni APEI 7 (S) S 3132 4.8877, -141.7569 NHMUK 2021.18 CCZ_062 ON400689 (COI), ON406641 (18S)
Ophiuroidea Ophioscolecida Ophioscolecidae Ophiocymbium tanyae APEI 1 (AP) S 5204 11.2523, -153.5848 NHMUK 2022.74 CCZ_206 ON406633 (18S), ON406596 (28S)
Ophiocymbium cf. rarispinum APEI 1 (AP) S 5206 11.2518, -153.6059 NHMUK 2022.73 CCZ_197 ON400727 (COI)
Ophiurida Ophiopyrgidae Ophiuroglypha cf. irrorata APEI 7 (S) S 3239 4.9081, -141.6813 NHMUK 2021.21 CCZ_058 ON400685 (COI)
APEI 7 (S) S 3096 4.8897, -141.7500 NHMUK 2022.72 CCZ_059 ON400686 (COI)
Porifera Hexactinellida Amphidiscosida Hyalonematidae Hyalonema stet. CCZ_020 APEI 7 (AP) Sa 4856 5.1149, -141.8967 NHMUK CCZ_020 ON400683 (COI), ON406634 (18S), ON406608 (16S), ON406597 (28S), ON411254 (ALG11)
APEI 1 (AP) Sa 5245 11.2954, -153.7422 NHMUK 2022.8 CCZ_179 ON400721 (COI), ON406609 (16S)
Hyalonema stet. CCZ_081 APEI 4 (AP) Sa 5031 7.0360, -149.9395 NHMUK 2022.9 CCZ_081 ON406610 (16S)
Lyssacinosida Euplectellidae Euplectellinae stet. CCZ_199 APEI 1 (AP) Sa 5202 11.2518, -153.5853 NHMUK CCZ_199 ON400729 (COI), ON406611 (16S)
Docosaccus sp. CCZ_021 APEI 7 (AP) Sa 4860 5.1043, -141.8867 NHMUK 2022.6 CCZ_021 ON400684 (COI), ON406635 (18S), ON406612 (16S), ON406598 (28S), ON411255 (ALG11)
Lyssacinosida Euplectellidae Holascus stet. CCZ_078 APEI 7 (AP) Sa 4874 5.0443, -141.8162 NHMUK 2022.7 CCZ_078 ON400700 (COI), ON406636 (18S), ON406613 (16S), ON406599 (28S), ON411256 (ALG11)
Bolosominae stet. CCZ_198 APEI 1 (AP) Sa 5205 11.2518, -153.6053 NHMUK 2022.10 CCZ_198 ON400728 (COI), ON406637 (18S), ON406614 (16S), ON406600 (28S)
Sceptrulophora Euretidae Bathyxiphus sp. CCZ_151 APEI 4 (AP) B 5001 6.9881, -149.9321 NHMUK CCZ_151 ON400713 (COI), ON406638 (18S), ON406615 (16S), ON406601 (28S)

Only two of these nine species had been previously found in the CCZ. Juveniles of the brittle star Ophiocymbium tanyae Martynov, 2010 were collected in the eastern IFREMER contract area and in APEI 3, but due to their early life stage, they lacked taxonomically informative characters and were only assigned to family level using DNA barcoding data (Christodoulou et al. 2020). In this study, genetic data confirmed the taxonomic identity of these specimens. The other species previously known from the CCZ is the sea cucumber Peniagone leander Pawson & Foell, 1986, which also occurs in the Mariana Trench (Gong et al. 2020). Additionally, ten species were assigned as ‘cf.’ based on morphological differences from similar described species, or because prior collections were in other ocean basins or different bathymetric ranges. These, and the remaining 30 taxa, likely represent undescribed species. Based on morphological and genetic evidence, two of these undescribed taxa have also been previously reported for the eastern CCZ (Freyastera cf. benthophila and Crinoidea sp. NHM_055 from Glover et al. (2016b) and (Amon et al. 2017b), referred to herein as Freyastera cf. tuberculata and cf. Porphyrocrinus sp. CCZ_165, respectively).

The in situ images taken for 53 specimens were classified into a total of 45 morphotypes using the standardised megafauna imagery catalogue (Simon-Lledó et al., pers. obs.). From these, 11 (24%) were new additions to the existing catalogue, thus representing morphotypes exclusively (to-date) encountered in the western CCZ (i.e., APEIs 1, 4 and 7), while 27 (60%) had already been encountered in other areas. More specifically, nine (20%) of the 45 morphotypes encountered in the western CCZ have also previously been found both in abyssal areas of the Kiribati EEZ (west of the areas studied) and in the eastern CCZ. Two (4%) of the morphotypes encountered in the western CCZ have been found in Kiribati (but not in eastern CCZ locations), whereas 16 (36%) of the western CCZ morphotypes have been encountered in the eastern CCZ, but not in Kiribati.

Descriptions

Phylum Annelida Lamarck, 1809

Class Polychaeta Grube, 1850

Subclass Errantia Audouin & H Milne Edwards, 1832

Order Phyllodocida Dales, 1962

Suborder Aphroditiformia Levinsen, 1883

Family Aphroditidae Malmgren, 1867

Genus Laetmonice Kinberg, 1856

Currently, there are no records from ≥ 3000 m depth for the genus Laetmonice Kinberg, 1856, in the Clarion-Clipperton Zone (OBIS 2022). A single polychaete specimen was collected, for which the genetic sequence of the COI gene was generated and used to estimate a COI-only phylogenetic tree (Fig. 2).

Figure 2. 

Rooted Bayesian phylogeny for the family Aphroditidae. COI-only BEAST median consensus tree with posterior probability (PP) and bootstrap (BS) values indicated for each node. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.

Laetmonice stet. CCZ_060

Fig. 3

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8897°N, 141.75°W; 3096 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400687 (COI); NHMUK 2022.76; Voucher code: CCZ_060.

Description

Single specimen (Fig. 3A). Body short, ovoid, flattened ventrally and somewhat arched dorsally. Specimen ~ 1 cm at widest point and 2 cm long, with 31 chaetigers. Dorsal felt not present. Specimen caked dorsally in dense layer of pale sediment (Fig. 3B, E), easily removed from dorsum but adhering to prostomium, parapodia, chaetae, and pygidium, obscuring respective features. Elytra 15 pairs, semi-translucent, smooth, and overlapping to cover dorsum (Fig. 3C). Dorsal cirri long, fine and tapering, extending beyond parapodia. Ventrum smooth. Ventral cirri, short, mostly broken off, not extending to base of neurochaetae. Parapodia biramous. Notochaetae include long, dark, brassy spines (Fig. 3E) with simple, tapered tips or with harpoon-shaped tips bearing four or five recurved fangs (Fig. 3D); both types of notochaetae with tuberculated shafts (Fig. 3G); neurochaetae include finer, shorter, paler chaetae with subdistal lateral spur and distal fringe of filamentous hairs (Fig. 3F), tips frequently broken off or covered in sediment.

Figure 3. 

Laetmonice stet. CCZ_060 A in situ image B ventral surface C elytra on dorsal surface D harpoon-shaped chaeta E dorsal surface F neurochaeta with fringed tips G notochaetal spine shafts. Scale bars: 2cm (A); 0.5 cm (B, E). Image attribution: Durden and Smith (A), Wiklund, Durden, Drennan, and McQuaid (B, E), Drennan (C, D, F, G).

Remarks

The presence of harpoon-shaped notochaetae supports the placement of this specimen within the genus Laetmonice (Fauchald 1977). Forms a monophyletic clade with other species of the genus Laetmonice based on COI sequences. Genetically distinct from Laetmonice stet. CCZ_060, the closest match is with Laetmonice filicornis Kinberg, 1856 (90.8% similarity). Laetmonice filicornis is described from shelf depths near Sweden in the North Atlantic.

Ecology

This specimen was observed crawling on the sedimented seafloor on the seamount of APEI 7 at 3096 m depth.

Comparison with image-based catalogue

No exactly identical Aphroditiformia morphotypes have been so far catalogued from seabed imagery collected in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Laetmonice stet. CCZ_060 was added as a new morphotype (i.e., Laetmonice sp. indet., ANN_019) in the megafauna imagery catalogue. Only one other Aphroditiformia morphotype (i.e., Aphroditidae gen. indet., ANN_022; with much larger spines and no sediment coating), was catalogued from seabed imagery in the eastern CCZ, also found on a seamount. In vertically-facing seabed images, Aphroditiformia morphotypes could potentially be confused with plate-shaped Xenophyophore tests (e.g., Psamminidae), particularly a dense layer of sediment is found coating specimens, as observed in Laetmonice stet. CCZ_060 (Fig. 3A).

Phylum Arthropoda von Siebold, 1848

Subphylum Crustacea Brünnich, 1772

Superclass Multicrustacea Regier, Shultz, Zwick, Hussey, Ball, Wetzer, Martin & Cunningham, 2010

Class Thecostraca Gruvel, 1905

Subclass Cirripedia Burmeister, 1834

Infraclass Thoracica Darwin, 1854

Superorder Thoracicalcarea Gale, 2015

Order Scalpellomorpha Buckeridge & Newman, 2006

Family Scalpellidae Pilsbry, 1907

To date, there is a single record at > 3,000 m depth for the order Scalpellomorpha in the CCZ (OBIS 2022), but no collected material. Three specimens were collected during the DeepCCZ expedition; these belong to three different species from which only one was confidently assigned to a previously described species. Sequences for the COI and 18S genes were generated for the three specimens and included in a phylogenetic tree estimated from 18S and COI sequences (Fig. 4).

Figure 4. 

Rooted Bayesian phylogeny of Scalpellomorpha. Concatenated (18S, and COI) BEAST median consensus tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.

Scalpellomorpha have been commonly found in image-based megafauna surveys across the north Pacific abyss, usually attached to sponge stalks or nodules. However, their classification beyond family level (e.g., Scalpellidae) from seabed imagery is constrained by their generally small size; only large specimens (> 3 cm) which are rarely encountered can sometimes be classified to genus level from in situ images. Consequently, scalpellid specimens usually are collated into a single, generic morphotype (i.e., Scalpellidae gen. indet., ART_010) in image-based quantitative analyses.

Genus Trianguloscalpellum Zevina, 1978

Trianguloscalpellum gigas (Hoek, 1883)

Fig. 5

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 5.0442°N, 141.8165°W; 4874 m deep; 28 May. 2018; Smith & Durden leg.; GenBank: ON400698 (COI), ON406624 (18S); WAM C74110; Voucher code: CCZ_074.

Description

Single specimen, found attached to a glass sponge stalk (Fig. 5A). Capitulum elongated, longer than wide (L = 8 mm, W = 5 mm), white, with short peduncle (2 mm) covered by large scales (Fig. 5B, C). Capitulum is formed by 14 capitular plates, and growth lines are not visible. Carina is simply bowed, narrowing distally but being approx. the same breath proximally. The tergum is somewhat oval-shaped, long, ~ 2× as long as wide, with pointed basal angle, carinal margin arched, and occludent margin straight. Scutum is somewhat quadrangular, broad, 1.5× as long as wide, with occludent margin much longer than the lateral margin. Inframedian latus is triangular, reaching upper latus. Carinolatus triangular, umbo apical, higher than rostrolatus.

Figure 5. 

Trianguloscalpellum gigas (Hoek, 1883). Specimen CCZ_074: A in situ photograph, attached to a glass sponge stalk B left C and right lateral views. Scale bars: 5 cm (A); 1 mm (B, C). Image attribution: Durden and Smith (A), Hosie (B, C). Arrows indicate position of T. gigas (specimen CCZ_074; lower, yellow) and Catherinum cf. albatrossianum (specimen CCZ_073; upper, green).

Remarks

The specimen appears to be a juvenile of the species T. gigas based on the plate arrangement, although diagnostic characters are not fully developed. There are no sequences available on public databases for T. gigas, but the 18S gene sequence is very similar (> 99%) to other species within the family Scalpellidae, mostly within the subfamily Arcoscalpellinae. However, the COI sequence is highly divergent (> 15% nucleotide divergence and > 3% amino-acid divergence) from published sequences of other species within the subfamily. The phylogenetic tree from concatenated data for COI and 18S recovered a well-supported clade of species of Anguloscalpellum and Trianguloscalpellum, but did not recover the genera as monophyletic. The type material for T. gigas was collected during the H.M.S. Challenger expedition in the middle of the North Pacific (Station 246: 36.1667°N, 178.0°E) at 3749 m depth (Hoek 1883). The species has been recorded from the Northwest and Southwest Pacific, and the Indian Ocean, from 3310 to 4820 m depth (Shalaeva and Boxshall 2014).

Ecology

The specimen was collected in the sedimented abyssal plain of APEI 7, at 4874 m depth. It was attached to a glass sponge stalk, along with another barnacle (Catherinum cf. albatrossianum; specimen CCZ_073), and an anemone (Metridioidea stet. CCZ_072; specimen CCZ_072).

Comparison with image-based catalogue

No exactly similar Scalpellidae morphotypes have been so far catalogued from seabed imagery collected in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Trianguloscalpellum gigas was catalogued as a new morphotype (i.e., Trianguloscalpellum gigas sp. inc., ART_033). However, given the small size of specimen CCZ_074, this morphotype could have easily been i.e., undetected in seabed image surveys conducted in other areas of the CCZ.

Genus Catherinum Zevina, 1978

Catherinum cf. albatrossianum (Pilsbry, 1907)

Fig. 6

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 5.0442°N, 141.8165°W; 4875 m deep; 28 May. 2018; Smith & Durden leg.; GenBank: ON400697 (COI), ON406623 (18S); WAM C74109; Voucher code: CCZ_073.

Description

Single specimen 21 mm long, attached to glass sponge stalk (Fig. 5A; upper, green arrow). Capitulum elongated, white, ~ 2× as long as wide (L = 16 mm, W = 8 mm); widest in the middle, tapering towards summit and base; short peduncle (4 mm) with small scales (Fig. 6A, B). Fourteen capitular plates fully calcified, showing growth lines, and separated by very narrow chitinous spaces. Carina is strongly arched in the distal half, tapering proximally, with flat roof and apical umbo. Tergum is almost a right triangle, longer than wide, with slightly convex occludent margin. Scutum is more than twice as wide as long, with arcuate occludent margin, with a distal indent on the lateral margin for the reception of the apex of the upper latus; baso-lateral margin rounded and next to the infra-median latus. Upper latus is pentagonal; with apical umbo projecting into notch on the scutum; scutal margin in concave; very short basal margin and carinolateral margin longer than carinal margin. Rostrolatus has an umbo projecting from the rostral margin. Rostrum minute. Large carinolatus, ~ 2× as long as wide, umbo sub-basal, abutting base of carina, apex slightly extending approximately one fifth of the carina. Inframedian latus is > 2× as long as the widest section, widest distally and with rostral and carinal margins concave, with umbo sub-basal.

Figure 6. 

Catherinum cf. albatrossianum (Pilsbry, 1907). Specimen CCZ_073: A left B right lateral views. Scale bars: 2 mm. Image attribution: Hosie (A, B).

Remarks

Morphological characters are in accordance with the description of C. albatrossianum. The 18S sequence matches three genera within the subfamily Arcoscalpellinae Zevina, 1978, while the closest match (85% similarity) for the COI sequence is to another species of Catherinum. Like, C. cf. novaezelandiae it differs morphologically from C. tortilum, reported from the CCZ by Poltarukha and Mel’Nik (2012), in the form of the inframedian latus. The type locality of C. albatrossianum is off Cape Hatteras, in the northwest Atlantic, at ~ 3740 m depth, but it has been reported for the North Atlantic, Gulf of Mexico, and Indian Ocean between 760 and 4180 m depth (Zevina and Poltarukha 2014). The original description states that the species lacks a rostrum, however, a minute rostrum is present in the specimen examined herein. This in addition to the documented range of this species is the reason for the use of cf. in the identification.

Ecology

Specimen was collected in a muddy abyssal area of APEI 7, at 4874 m depth. It was attached to a glass sponge stalk (Fig. 5A; upper, green arrow), along with another barnacle (Trianguloscalpellum gigas, specimen CCZ_074; lower, yellow arrow), and an anemone (Metridioidea stet. CCZ_072; specimen CCZ_072). It had hydrozoans and two serpulid polychaetes attached to it.

Comparison with image-based catalogue

A very similar morphotype (Catherinum sp. indet., ART_032) has been encountered (e.g., large specimens > 3 cm in length) in seabed image surveys conducted across the eastern CCZ and in abyssal areas of the Kiribati EEZ.

Catherinum cf. novaezelandiae (Hoek, 1883)

Fig. 7

Material

Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2751°N, 153.7444°W; 5241 m deep; 09 Jun. 2018; Smith & Durden leg.; GenBank: ON400722 (COI), ON406625 (18S); WAM C74111; Voucher code: CCZ_185.

Description

Single specimen 14 mm long; with elongated, white capitulum, > 2× as long as wide (L = 12 mm, W = 5 mm), and short peduncle (2 mm) with small scales (Fig. 7). Capitulum consists of 14 fully calcified capitular plates with growth lines, separated from each other by narrow chitinous sutures. Carina is simply bowed, with flat roof. Tergum is triangular, shorter on the occludent margin, with apical umbo; apical angle is similar to angle between the carinal and scutal margins. Upper latus somewhat pentagonal, with lower edge truncated, and apical edge reaching over the scutum; with apical umbo. Rostrolatus with umbo apical on the rostral margin, and arched lateral margin. Inframedian latus irregular in shape, narrow, almost 3× as long as the widest part, with umbo sub-medial; rostral and carinal margins concave. Carinolatus is large, ~ 2× as long as wide, with umbo sub-carinal, above basal angle.

Figure 7. 

Catherinum cf. novaezelandiae (Hoek, 1883). Specimen CCZ_185: A in situ photograph B right C left lateral views. Scale bars: 1 cm (A); 1 mm (B, C). Image attribution: Durden and Smith (A), Hosie (B, C).

Remarks

Morphological characters of the capitulum conform to the description of the genus Catherinum. The sequence for the 18S gene is similar to sequences from other species within the same family. Another species within the genus, C. tortilum (Zevina, 1973), originally described from the Indian Ocean at 2760 m depth has also been recorded for the CCZ at similar depths (4872–4877 m depth; Poltarukha and Mel’Nik 2012). In C. tortilum, the inframedian latus’ umbo is conspicuously displaced laterally away from the midline. The species C. novaezelandiae is distributed in the Western and Eastern Indian Ocean, Western Central and Southwest Pacific, from depths 455–4800 m (Shalaeva and Boxshall 2014), but was originally described from East Cape, New Zealand (Southwest Pacific), at 1280 m.

Ecology

The specimen was collected in the sedimented abyssal plain of APEI 1 at 5241 m depth. It was attached to a glass sponge stalk, along with a crinoid (Bathymetrinae inc. CCZ_176; specimen CCZ_186), a polychaete, and anemones, that was anchored in the mud.

Comparison with image-based catalogue

Relatively large abundances of a very similar morphotype (Catherinum sp. indet., ART_031) were observed in seabed imagery collected within abyssal areas of the Kiribati EEZ, but not in eastern CCZ surveys.

Phylum Cnidaria Hatschek, 1888

A total of 12 cnidarians w collected, belonging to six orders in two classes (Anthozoa and Scyphozoa).

Class Anthozoa Ehrenberg, 1834

Subclass Hexacorallia Haeckel, 1896

Order Actiniaria Hertwig, 1882

To date, there are 33 records of Actiniaria found at > 3000 m depth in the CCZ (OBIS 2022), but only two of these represent collected specimens. We collected five specimens, all belonging to different species, and for which genetic sequences of the COI or 18S genes were generated and included in a phylogenetic tree built from a concatenated alignment of 12S, 16S, 18S, 28S, COI, and COX3 (Fig. 8).

Figure 8. 

Rooted Bayesian phylogeny of Actiniaria. Concatenated (12S, 16S, 18S, 28S, COI, and COX3) BEAST median consensus tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.

Suborder Enthemonae Rodríguez & Daly in Rodríguez et al. 2014

Superfamily Metridioidea Carlgren, 1893

Metridioidea stet. CCZ_072

Fig. 9

Material. Clarion-Clipperton Zone • 1 specimen; APEI 1; 5.0442°N, 141.8165°W; 4875 m deep; 28 May. 2018; Smith & Durden leg.; GenBank: ON400696 (COI); NHMUK 2021.19; Voucher code: CCZ_072.

Description. Single specimen, white (Figs 5, 9). Body subcylindrical, pedal disc modified and attached to a glass sponge stalk, oral disc is > 2× column width; with at least two cycles of slender, tapered, long, white tentacles, almost as long as the oral disc diameter (Fig. 5A). Tubercles are evident on the top half of the column when preserved, but tentacles completely retracted (Fig. 9 A, B).

Figure 9. 

Metridioidea stet. CCZ_072 A oral B lateral views. Scale bars: 5 mm (A, B). Image attribution: Wiklund, Durden, Drennan, and McQuaid (A, B).

Remarks. COI sequence is similar (97.3%) to other species within the subfamily Metridioidea but based on COI we were unable to delimit species because interspecific divergence is very low. Additionally, only a few studies have included sequences for COI, therefore hindering comparisons based solely on this gene. The COI divergence between Metridiodea stet. CCZ_164 and Metridiodea stet. CCZ_072 (1.95% K2P distance) was higher than the genetic distance between other species in the family Metridiodea (Rodriguez et al. 2014), suggesting these to belong to separate species. The phylogenetic tree recovered both CCZ specimens within the subfamily Metridioidea (Fig. 8), in a clade belonging to Cuticulata. Clades within Cuticulata were not well resolved in the phylogeny, but this group includes the Graspina clade (families Amphiantidae, Galantheanthenidae, and Actinoscyphiidae) that is characterised by a modified pedal disc that enables them to attach to other substrates, such as sponge stalks (Rodriguez et al. 2014), and is advantageous in deep-sea ecosystems. Based on this modified pedal disc, the specimen very likely belongs to a family within the Graspina clade.

Ecology. The specimen was collected in a muddy abyssal plain in APEI 7, at 4874 m depth. It was attached to a glass sponge stalk (Fig. 5A; top of stalk), along with two barnacles (Catherinum cf. albatrossianum, specimen CCZ_073; and Trianguloscalpellum gigas, specimen CCZ_074).

Comparison with image-based catalogue. A very similar Actiniaria morphotype (Metridioidea fam. indet., ACT_042) mostly attached to sponge stalks, has been commonly encountered in seabed image surveys conducted across the eastern CCZ but not in abyssal areas of the Kiribati EEZ.

Metridioidea stet. CCZ_154

Fig. 10

Material. Clarion-Clipperton Zone • 1 specimen; APEI 4; 6.9702°N, 149.9426°W; 5009 m deep; 06 Jun. 2018; Smith & Durden leg.; GenBank: ON400715 (COI); NHMUK 2021.27; Voucher code: CCZ_154.

Description. Single specimen, completely white when alive (Fig. 10). Body of live specimen is more or less cylindrical, wider proximally and distally, 29 mm long. Pedal disc is the widest, 35 mm in diameter, attached to a manganese nodule, and oral disc 24 mm in diameter (Fig. 10B). Large and small conical, tapered tentacles alternating on the margin of the oral disc in two cycles: ~ 20 + 20, with the larger ones being approx. half the oral disc diameter and located above the smaller tentacles (Fig. 10A). Tentacles are only visible in in situ images (Fig. 10A), as they are fully retracted in the preserved specimen.

Figure 10. 

Metridioidea stet. CCZ_154 A in situ image B specimen before preservation. Scale bars: A, B 1 cm. Image attribution: Durden and Smith (A), Wiklund, Durden, Drennan, and McQuaid (B).

Ecology. This specimen was attached to a nodule in abyssal sediments in APEI 4 at 5009 m depth.

Remarks. The COI sequence is similar to sequences of species within different families, but in the phylogenetic tree it is recovered within the superfamily Metridioidea (Fig. 8).

Comparison with image-based catalogue. No similar Actiniaria morphotypes had been catalogued so far from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. The in situ image of Metridiodea stet. CCZ_154 was hence catalogued as a new morphotype (i.e., Metridioidea fam. indet., ACT_044).

Metridioidea stet. CCZ_164

Fig. 11

Material. Clarion-Clipperton Zone • 1 specimen; APEI 7; 6.988°N, 149.9326°W; 5001 m deep; 06 Jun. 2018; Smith & Durden leg.; GenBank: ON400717 (COI); NHMUK 2021.5; Voucher code: CCZ_164

Description. Single specimen, white (Fig. 11A). Specimen with a short, subcylindrical column, with pedal and oral discs almost the same diameter (Fig. 11A). Long, slender, tapered, white tentacles arranged in at least two cycles (Fig. 11A). When preserved, column is more cylindrical, almost as long as wide (H = 18 mm, oral disc diameter = 21 mm), and tubercles are evident on the top half of the column; tentacles completely retracted (Fig. 11B, C).

Figure 11. 

Metridioidea stet. CCZ_164 A in situ image of specimen CCZ_164 B detail of oral disc C lateral view of specimen. Scale bars: 2 cm (A); 2 mm (B); 5 mm (C). Image attribution: Durden and Smith (A), Wiklund, Durden, Drennan, and McQuaid (B, C).

Remarks. COI sequence is very similar to Metridiodea sp. CCZ_072 and they are recovered as sister species, in the multi-gene phylogeny, within the Cuticulata in the superfamily Metridioidea (Fig. 8). This species very likely belongs to a family within the Graspina clade (Amphiantidae, Galantheanthenidae and Actinoscyphiidae) based on the modified pedal disc that allows them to attach to substrates other than rocks (Rodriguez et al. 2014).

Ecology. This specimen was collected in muddy abyssal sediments in APEI 4 at 5001 m depth, attached to a glass sponge stalk.

Comparison with image-based catalogue. As with specimen from Metridiodea stet. CCZ_072, a very similar morphotype has been commonly found in seabed image surveys conducted across the eastern CCZ (i.e., Metridioidea fam. indet., ACT_042), but it does not seem possible to differentiate between the species Metridiodea stet. CCZ_072 and Metridiodea stet. CCZ_164 from in situ imagery. Morphotype ACT_042 is hence likely to encompass, at least, these two species in image-based analyses conducted across the CCZ.

Superfamily Actinostoloidea Carlgren, 1932

Family Actinostolidae Carlgren, 1932

Actinostolidae stet. CCZ_183

Fig. 12

Material. Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2751°N, 153.7444°W; 5241 m deep; 09 Jun. 2018; Smith & Durden leg.; GenBank: ON406626 (18S); NHMUK 2021.28; Voucher code: CCZ_183.

Description. Single specimen, white, attached to a nodule (Fig. 12A). Column is very short (3 mm), cylindrical (6 mm diameter), pedal disc much wider and completely attached to the nodule. Small tubercles scatter on the column (Fig. 12B).

Figure 12. 

Actinostolidae stet. CCZ_183 A specimen attached to nodule B close-up of specimen. Scale bars: 1 cm (A), 2 mm (B). Image attribution: Wiklund, Durden, Drennan, and McQuaid (A, B).

Remarks. Closest matches for the 18S sequence are sequences from other members of the family Actinostolidae (> 99.3%). In the phylogenetic tree, it is also recovered in a well-supported clade with species of the family Actinostolidae (Fig. 8). However, this clade also includes Capnea, which has been recovered within the same clade in previous studies (Rodriguez et al. 2014), and a specimen collected in the eastern CCZ identified as a member of the family Hormathiidae (Hormathiidae sp. NHM_416, Dahlgren et al, 2016). No in situ photos are available.

Ecology. This specimen was collected in abyssal sediment in APEI 1 at 5241 m depth, attached to a polymetallic nodule.

Actinostolidae stet. CCZ_202

Fig. 13

Material. Clarion-Clipperton Zone • 1 specimen; APEI 4; 11.2518°N, 153.6059°W; 5206 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON406627 (18S); NHMUK 2021.22; Voucher code: CCZ_202.

Description. Single, white specimen (Fig. 13A). Specimen with short column (4 mm), pedal and oral disc approx. the same diameter (8 mm). Between 8–10 white, long tentacles, approx. as long as the oral disc diameter (Fig. 13A). Column with scale-like pattern in preserved specimen (Fig. 13B), with tentacles fully retracted.

Figure 13. 

Actinostolidae stet. CCZ_202 A in situ image B detail of specimen. Scale bars: 1 cm (A); 1 mm (B). Image attributions: Durden and Smith (A), Wiklund, Durden, Drennan, and McQuaid (B).

Remarks. The closest matches to the 18S sequence are species in different suborders within the Actiniaria (98.3% sequence similarity), including Hormathiidae sp. NHM_416 from the CCZ (Dahlgren et al. 2016). However, in the phylogenetic tree it is confidently recovered within the Actinostolidae (Fig. 8), along with the specimen Hormathiidae sp. NHM_416.

Ecology. This specimen was attached to a polymetallic nodule collected in abyssal sediments of APEI 1 at 5206 m depth.

Comparison with image-based catalogue. No similar Actiniaria morphotypes have been so far catalogued from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. The in situ image of Actinostolidae stet. CCZ_202 was hence catalogued as a new morphotype (i.e., Actinostolidae gen. indet., ACT_080). However, small actiniarians (e.g., oral disc < 2 cm) are usually difficult to classify from seabed imagery as basic morphological features (e.g., number of tentacles) are often not clearly visible. Consequently, ACT_080 could be potentially confused with similarly small actinian morphotypes commonly encountered in the eastern CCZ (i.e., Hormathiidae gen. inc., ACT_022, also with a short pedal approx. the same diameter as the oral disc, but with 16–18 long thin tentacles).

Order Scleractinia Bourne, 1900

For Scleractinia, there are only two records at > 3000 m depth in the CCZ (OBIS 2022), with no specimens collected. A single scleractinian was collected, for which DNA amplification was unsuccessful.

Family Fungiacyathidae Chevalier & Beauvais, 1987

Genus Fungiacyathus Sars, 1872

Fungiacyathus (Fungiacyathus) cf.fragilis Sars, 1872

Fig. 14

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.2647°N, 149.774°W; 3562 m deep; 03 Jun. 2018; Smith & Durden leg.; NHMUK 2021.26; Voucher code: CCZ_107

Description

Single specimen, solitary, and unattached, ~ 27 mm in transverse diameter. Live specimen with tapered, transparent tentacles, longer than half the corallum diameter and arranged in two or three cycles (Fig. 14A). Corallum is light brown distally and darker proximally on live specimen (Fig. 14B, C). The base is flat and the lower cycle septa are strongly arched upward; septa are arranged in five cycles, those of the fifth are rudimentary.

Figure 14. 

Fungiacyathus (Fungiacyathus) cf. fragilis Sars, 1872. Specimen CCZ_107: A in situ image B dorsal view of live specimen C bleached skeleton. Scale bars: 1 cm (A); 3 mm (B, C). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B); Bribiesca-Contreras (C).

Remarks

No genetic sequences were obtained from this specimen. Morphological characters match the genus Fungiacyathus.

Ecology

This free-living specimen was found on a sedimented area on a seamount in APEI 4, at 3561 m depth.

Comparison with image-based catalogue

A very similar scleractinian morphotype (i.e., Fungiacyathus sp. indet., SCL_003) has been encountered in seabed image surveys conducted across the eastern CCZ but not in abyssal areas of the Kiribati EEZ, usually on sediment. As with other solitary scleractinians, this taxon could be confused with an anemone in seabed imagery (e.g., SCL_003 was originally catalogued as an Actiniaria from in situ images, which was addressed following the collection and analysis of the specimen collected in this study).

Subclass Octocorallia Haeckel, 1866

Order Alcyonacea Lamouroux, 1812

There are 131 records of Alcyonacea at > 3000 m depth in the CCZ, only eight of those representing preserved specimens (OBIS 2022). We collected three specimens belonging to three different species, only one assigned to a previously described species. Genetic sequences for both 16S and COI genes were amplified for each specimen, and included in a concatenated alignment (16S, COI, mtMutS, NADH2) used to generate a phylogenetic tree of Octocorallia (Fig. 15). Classification of Alcyonacea specimens from seabed imagery is often constrained by the lack of visibility of polyp morphology, particularly when these are small (e.g., family Primnoidae). Therefore, classification from in situ images is mostly based on broader features like the branching pattern, the length of the main stem, and/or the number, size, and positioning of polyps on branch nodes.

Figure 15. 

Rooted Bayesian phylogeny of Octocorallia. Concatenated (16S, COI, mtMutS, NADH2) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.

Suborder Calcaxonia Grasshoff, 1999

Family Chrysogorgiidae Verrill, 1883

Genus Chrysogorgia Duchassaing & Michelotti, 1864

Chrysogorgia sp. CCZ_112

Fig. 16

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.2874°N, 149.8578°W; 4125 m deep; 04 Jun. 2018; Smith & Durden leg.; GenBank: ON400711 (COI), ON406602 (16S); NHMUK; Voucher code: CCZ_112.

Description

Wide, long, sparsely branched colony, ~ 30 cm tall from the base (Fig. 16A, B). Polyps constricted basally on the neck (Fig. 16C–E), placed on internodes and absent from the main stem (Fig. 16A, B). Polyps are light orange when alive (Fig. 16C, D) and white after preservation (Fig. 16E). Sclerites near the polyp base are scale-like, but throughout the body and along the tentacle rachis are all elongate flat rods; sclerites are absent from the tentacle pinnules.

Figure 16. 

Chrysogorgia sp. CCZ_112 A, B in situ images of colony C closed polyp on live specimen D opened polyp on live specimen showing light orange colouration E closed polyp of preserved specimen. Scale bars: 2 cm (A); 0.5 mm (C–E). Image attribution: Durden and Smith (A, B); Wiklund, Durden, Drennan, and McQuaid (C, D); Bribiesca-Contreras (E).

Remarks

The sequence for the COI gene is 0% divergent from a sequence of a specimen of Chrysogorgia abludo Pante & Watling, 2011 (specimen NAS102-3, GenBank accession number GQ180138) collected at Nashville Seamount, New England Seamounts at 2246 m depth (Station 102; 34.5828°N, 56.8433°W) included as comparative material during the species description (Pante and Watling 2011). In octocorals, it has been found that COI evolves very slowly and therefore it is not suitable for species discrimination, with different species having the same haplotype (McFadden et al. 2011). Chrysogorgia abludo is distributed in the Atlantic Ocean, and morphological characters of the specimen collected in this study differ from the original description of C. abludo, as well as other species within the genus and hence considered a potentially new species In the phylogenetic tree (Fig. 15) the specimen was also recovered along with another specimen of Chrysogorgia, supporting its placement within the genus.

Comparison with image-based catalogue

No similar Alcyonacea morphotypes have been catalogued so far from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Chrysogorgia sp. CCZ_112 was catalogued as a new morphotype (i.e., Chrysogorgia sp. indet., ALC_017).

Ecology

The specimen was attached to polymetallic crust on the slope of a seamount in the APEI 4, at 4124 m depth.

Family Mopseidae Gray, 1870

Mopseidae sp. CCZ_088

Fig. 17

Material. Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.0089°N, 149.9109°W; 5018 m deep; 02 Jun. 2018; Smith & Durden leg.; GenBank: ON400705 (COI), ON406603 (16S); NHMUK XXX; Voucher code: CCZ_088.

Description. Single specimen, with white axis and polyps; polyps standing perpendicular to the axis when alive (Fig. 17A). Colony is long, ~ 45 cm tall, and unbranched (Fig. 17A, B). Polyps are tall, ~ 2 mm, clavate, and standing parallel to the branch (Fig. 17C).

Figure 17. 

Mopseidae sp. CCZ_088 A in situ image B whole colony attached to a nodule C detail of polyps before preservation. Scale bars: 5 cm (A); 2 cm (B); 5 mm (C). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B, C).

Remarks. Both 16S (0.3% K2P) and COI (0.6% K2) sequences are very similar to Mopseinae sp. NHM_330 (Dahlgren et al. 2016), which morphologically resembles the genus Primnoisis. The specimen from the western CCZ likely belongs to the same genus but based on genetic and morphological differences represents a different species from that of the eastern CCZ.

Ecology. The specimen was found attached to a nodule in abyssal sediments of APEI 4 at 5018 m depth.

Comparison with image-based catalogue. No similar Alcyonacea morphotypes had been catalogued so far from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of CCZ_088 was catalogued as a new morphotype (i.e., Mopseidae gen. indet., ALC_018). However, it is often not possible to determine whether such small and abundant polyps are arranged in pairs or not, or the actual orientation of these with regards to the axis from seabed images.

Family Primnoidae Milne Edwards, 1857

Genus Calyptrophora Gray, 1866

Calyptrophora distolos Cairns, 2018

Fig. 18

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.2874°N, 149.8578°W; 4125 m deep; 04 Jun. 2018; Smith & Durden leg.; GenBank: ON400712 (COI), ON406604 (16S); USNM 1550968; Voucher: CCZ_131.

Description

Branching uniplanar, colony ~ 20.8 cm tall, with polyps perpendicular to the stem in in situ images (Fig. 18A). Downward-oriented polyps, arranged parallel to the branch, mostly paired, but a few whorls with three to four polyps are present; polyps are ~ 2.7 mm tall and with an operculum longer than either of the body wall scales (Fig. 18B, C).

Figure 18. 

Calyptrophora distolos Cairns, 2018. Specimen CCZ_132: A in situ image B whole colony C detail of polyps before preservation. Scale bars: 2 cm (A, B); 5 mm (C). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B, C).

Remarks

Morphological characters are concordant with the description of Calyptrophora distolos (Cairns 2018). In addition to the paired polyps mentioned in the species description, this specimen also presents a few whorls with three or four polyps (Fig. 18C). Polyps are downward-oriented, therefore belonging to the wyvillei complex (Cairns 2018). The species is most similar to C. persephone Cairns, 2015, which has been described for the UK-1 and BGR areas in the CCZ (Cairns 2015). However, C. persephone is characterised as having polyps oriented upwards, therefore belonging to the japonica complex, and that are consistently arranged in whorls of three or four, with each basal scale bearing two prominent distal spines. Calyptrophora distolos was described from the Enigma Seamount, south of Guam, at 3737 m depth, and has also been recorded for American Samoa at 2994 m depth (Cairns 2018). There are no genetic sequences available for other specimens of C. distolos, but the sequences generated here cluster with other species of the genus (Fig. 15). However, the genus was not recovered as monophyletic.

Ecology

The specimen was found attached to a polymetallic crust on the slope of a seamount on APEI 4, at 4124 m depth.

Comparison with image-based catalogue

A similar primnoid morphotype (i.e., Calyptrophora distolos sp. inc., ALC_016) was catalogued from seabed imagery (also collected on a seamount) in the eastern CCZ (e.g., Cuvelier et al. 2020), but not in abyssal areas of the Kiribati EEZ.

Order Pennatulacea Verrill, 1865

A total of 79 records of Pennatulacea occurring at > 3000 m depth in the CCZ have been recorded in OBIS, but none represent preserved specimens (OBIS 2022). We recovered a single specimen, for which sequences of both 16S and COI genes were obtained, and which were included in the phylogenetic analysis of the Octocorallia (Fig. 15).

Suborder Sessiliflorae Kükenthal, 1915

Family Protoptilidae Kölliker, 1872

Genus Protoptilum Kölliker, 1872

Protoptilum stet. CCZ_068

Fig. 19

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8897°N, 141.75°W; 3096 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400694 (COI), ON406605 (16S); NHMUK 2021.24; Voucher: CCZ_068

Description

Single specimen, ~ 12 cm tall, narrow sea pen; in situ colouration orange with whitish polyps (Fig. 19A). Two rows, opposite to each other, of elongated polyp calyces along the rachis (Fig. 19B, C).

Figure 19. 

Protoptilum stet. CCZ_068 A in situ image B whole colony C detail of polyps before preservation. Scale bars: 1 cm (A); 5 mm (B); 2 mm (C). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B, C).

Remarks

The COI sequence forms a clade with sequences from Protoptilum (< 1% genetic divergence), a genus within the family Protoptilidae, while the 16S sequence is very similar to sequences of Protoptilum and Distichoptilum, both genera within the same family. In the phylogenetic tree, the family Protoptilidae was not recovered as monophyletic, but the CCZ specimen was recovered (with 1.00 posterior probability) as sister to Protoptilum carpenterii Kölliker, 1872.

Ecology

The specimen was found anchored to soft sediment on a seamount of APEI 7, at 3096 m depth.

Comparison with image-based catalogue

No similar Pennatulacea morphotypes have been catalogued so far from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Protoptilum stet. CCZ_068 was catalogued as a new morphotype (i.e., Protoptilum sp. indet., PEN_024). In seabed images, PEN_024 can resemble other single-branched sea pens or even soft corals.

Subclass Ceriantharia Perrier, 1893

Order Spirularia den Hartog, 1977

To date, there are no records from a minimum of 3000 m depth in the CCZ for the order Spirularia (OBIS 2022). We recovered a single specimen, for which the COI and 16S genes were successfully amplified and included in a concatenated matrix (12S, 16S, 18S, 28S, and COI) to estimate a phylogenetic tree of the Ceriantharia (Fig. 20).

Figure 20. 

Rooted Bayesian phylogeny of Ceriantharia. Concatenated (12S, 16S, 18S, 28S, and COI) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.

Spirularia stet. CCZ_067

Fig. 21

Material. Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8875°N, 141.7572°W; 3132 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400693 (COI), ON406606 (16S); NHMUK 2021.23; Voucher: CCZ_067.

Figure 21. 

Spirularia stet. CCZ_067 A in situ image B specimen before preservation. Scale bars: 1 cm (A); 2 mm (B). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B).

Description. Single specimen, unattached, tube-dweller with tentacles extended above the sediment in situ (Fig. 21A). Very long, conical, tapering, reddish brown tentacles; capitulum whitish when alive (Fig. 21A). Column is 12 mm in height and 5 mm in width excluding tentacles (Fig. 21B). Tube consisting of soft sediment.

Remarks. The closest matches to the COI and 16S sequences were sequences from other members of the family Cerianthidae: Pachycenrianthus, Cerianthus, Ceriantheromorphe. However, in the concatenated phylogeny, it forms a clade with Boctrunidifer sp. 1 and Ceriantheopsis americanus, belonging to the families Botrucnidiferidae and Cerianthidae, respectively (Fig. 20). As Forero-Mejia et al. (2019) recovered both families as non-monophyletic and a revision of these is suggested, we were unable to assign it to a family.

Ecology. The specimen was found buried in the sediment on a seamount in APEI 7, at 3132 m depth.

Comparison with image-based catalogue. A very similar Ceriantharia morphotype (i.e., Spirularia sp. indet., CER_001) has been commonly encountered in seabed image surveys conducted across the eastern CCZ, always found semi-buried with the tentacles extending above the sediment surface.

Class Scyphozoa Goette, 1887

For the class Scyphozoa, there are currently 128 records from > 3000 m depth in the CCZ, but none represent preserved specimens (OBIS 2022). We collected a single specimen, for which the sequence for the COI gene was successfully amplified and included in a multi-gene phylogeny (16S, 18S, 28S, and COI) of the Scyphozoa (Fig. 22).

Figure 22. 

Rooted Bayesian phylogeny of Scyphozoa. Concatenated (16S, 18S, 28S, and COI) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.

Subclass Discomedusae Haeckel, 1880

Order Somaeostomeae Agassiz, 1862

Family Ulmaridae Haeckel, 1880

Ulmaridae stet. CCZ_069

Fig. 23

Material. Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8876°N, 141.7572°W; 3133 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400695 (COI); NHMUK 2021.25; Voucher: CCZ_069.

Description. Single specimen, ~ 4.5 cm in diameter; with transparent bell and light brown tentacles in situ (Fig. 23A). Rhopalia are evident around the bell (Fig. 23B, C).

Figure 23. 

Ulmaridae stet. CCZ_069 A in situ image B specimen before preservation C rhopalia. Scale bars: 1 cm (A); 5 mm (B). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B, C).

Remarks. Only the sequence for the COI gene was successfully amplified, but none of the matches on public databases were informative. In the phylogenetic tree (Fig. 22), the CCZ specimen was recovered in a clade with other members of the class Discomedusae. As both the Rhizostomeae and Semaeostomeae were not well supported, the specimen was not confidently assigned to any of both orders based on COI only. However, the specimen morphologically resembles an undescribed ulmariid scyphozoan ( Somaeostomeae) that was observed in the New Britain Trench (Gallo et al. 2015).

Ecology. The specimen was found on the sediment of a seamount in APEI 7 at 3095–3132 m depth. A similar species of ulmariid from the New Britain Trench was found to skim the seafloor to feed on particulates on the sediment (Gallo et al. 2015).

Comparison with image-based catalogue. No similar Ulmaridae morphotypes have been catalogued so far from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Ulmaridae stet. CCZ_069 was catalogued as a new morphotype (i.e., Ulmaridae gen. indet., SCY_010). A similarly shaped Ulmaridae morphotype (e.g., Ulmaridae gen. indet., SCY_009; opaque reddish bell, dark brown tentacles encircled with a white ring, and dark rhopalia around the bell), also eventually found crawling on the seabed surface, was previously catalogued from seabed imagery in nodule field areas of the eastern CCZ. When photographed lying on the seabed (as opposed to swimming in the water column), SCY_019 and SCY_010 may resemble an anemone, particularly in images collected at high altitude above the seabed (e.g., > 5 m).

Phylum Echinodermata

Class Asteroidea de Blainville, 1830

There are currently 245 records of sea stars occurring at a minimum of 3000 m depth in the CCZ, with only five of those representing preserved specimens (OBIS 2022). Four specimens were collected on the western CCZ, and sequences of the barcoding gene COI were generated for all of them, and 16S for a single specimen. These were included in a concatenated alignment of 12S, 16S, 18S, COI, and H3 used to estimate a phylogenetic tree (Fig. 24).

Figure 24. 

Rooted Bayesian phylogeny of Asteroidea. Concatenated (12S, 16S, 18S, COI, and H3) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.

Superorder Forcipulatacea Blake, 1987

Order Brisingida Fisher, 1928

Family Freyellidae Downey, 1986

Genus Freyastera Downey, 1986

Freyastera cf. tuberculata (Sladen, 1889)

Fig. 25

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 6.9879°N, 149.9123°W; 5000 m deep; 02 Jun. 2018; Smith & Durden leg.; GenBank: ON400716 (COI); NHMUK 2022.80; Voucher code: CCZ_157 • 1 specimen; APEI 4; 6.9873°N, 149.9331°W; 5000 m deep; 06 Jun. 2018; Smith & Durden leg.; GenBank: ON400704 (COI); NHMUK 2022.79; Voucher code: CCZ_087.

Comparative material

Pacific Ocean • 1 specimen, holotype of Freyella benthophila Sladen, 1889; mid-South Pacific; 39.6833°S, 131.3833°W; 4663 m deep; Challenger Expedition, Stn. 289; NHMUK 1890.5.7.1078. Atlantic Ocean • 1 specimen, syntype of Freyella tuberculata Sladen, 1889; between west coast of Africa and Ascencion Islands; 22.3°N, 22.0333°W; 4389 m deep; Challenger Expedition, Stn. 346; NHMUK 1890.5.7.1077. • 1 specimen, syntype of Freyella tuberculata; between Canary Islands and Cape Verde Islands; 2.7° S, 14.6833°W; 4298 m deep; Challenger Expedition, Stn. 89; NHMUK 1890.5.7.1076.

Description

Two specimens (R = 106 mm, r = 3 mm; R = 164 mm, r = 6 mm); live specimens whitish on both actinal and abactinal surfaces, tube feet transparent with bright orange flattened discs (Fig. 25A, B). Disc is small, somewhat rounded, slightly orange on actinal and abactinal surfaces (Fig. 25E, F); with six long, slender arms (Fig. 25A–C); lacking furrow spines (Fig. 25D). Each abactinal plate on the genital area bears a single spinelet (Fig. 25E), covered with a membrane with pedicellariae. Each mouth plate has two oral spines covered by a clear membrane bearing pedicellariae (Fig. 25F); one located on the adoral margin of the mouth plate and the suboral spine located above the centre of the mouth plate.

Figure 25. 

Freyastera cf. tuberculata (Sladen, 1889). Specimen CCZ_175: A in situ image C whole specimen D ventral surface of the arms before preservation. Specimen CCZ_087: B in situ image E details of dorsal disc F ventral disc surface before preservation. Scale bars: 2 cm (A, B); 1 cm (C); 2 mm (D); 1 mm (E); 0.5 mm (F). Image attribution: Durden and Smith (A, B); Wiklund, Durden, Drennan, and McQuaid (C–F).

Remarks

The COI sequences were very similar to sequences of Freyastera cf. benthophila (Sladen, 1889) collected in the UK-1 contract area from the CCZ (Glover et al. 2016a), and which were recovered in a single clade (Fig. 24). Only arm segments were recovered from the UK-1 specimens, and although they were found to resemble F. benthophila, the whole specimens collected in the western CCZ differ from the original description for the species. Only five species are known for having six rays: F. sexradiata (Perrier, 1885), F. benthophila, F. tuberculata (Sladen, 1889), F. basketa Zhang et al., 2019, and F. delicata Zhang et al., 2019. However, F. benthophila is easily distinguished from the other two species by its abactinal armament; each abactinal plate bearing two or three spinelets covered with a simple membrane with no pedicellariae (Sladen 1889). The specimens from the CCZ have abactinal spinelets covered by a membrane that bears pedicellariae (Fig. 25E). They also differ from F. benthophila in having the spines on the adoral margin of the mouth-plates covered by a clear membrane bearing pedicellariae instead of an opaque membrane with no pedicellariae (Downey 1986). In addition, the suboral spines are located above the centre of the mouth plate (Fig. 25F), as in F. tuberculata, and not below the centre of the mouth plate as described for F. benthophila (Downey 1986). Syntypes from F. tuberculata are from the Atlantic Ocean (Sladen 1889), but it has been reported for the Eastern Tropical Pacific (0.05°N, 117.25°W) at 4243 m depth (Downey 1986). Unfortunately, there are no genetic sequences available for this species, but COI sequences from CCZ specimens are highly divergent from the sequence of F. benthophila collected in the Mariana Trench (K2P distance: 13–14%). In the phylogenetic tree they are also recovered in different clades, very close to another species reported herein, and to F. delicata and Freyastera sp. Yap (in Zhang et al. 2019) (Fig. 24). The specimen collected here represents the same species as found in the eastern CCZ, Freyastera cf. benthophila (Glover et al. 2016b).

Ecology

One specimen was observed on the sedimented seafloor (CCZ_157), while another was sitting on a nodule with the actinal surface against the muddy seafloor and lifting the tip of the arms like a basket (CCZ_087). Both seastars were collected on abyssal sediments of APEI 4 at 5000 m depth. During morphological examination of these samples, the exoskeleton of a large (> 6 mm long), digested copepod was found in the stomach of specimen CCZ_157.

Freyastera stet. CCZ_201

Fig. 26

Material

Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2518°N, 153.6059°W; 5204 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON400730 (COI); NHMUK 2022.81; Voucher code: CCZ_201.

Comparative material

Pacific Ocean • 1 specimen, holotype of Freyella benthophila Sladen, 1889; mid-South Pacific; 39.6833°S, 131.3833°W; 4663 m deep; Challenger Expedition, Stn. 289; NHMUK 1890.5.7.1078. Atlantic Ocean • 1 specimen, syntype of Freyella tuberculata Sladen, 1889; between west coast of Africa and Ascencion Islands; 22.3°N, 22.0333°W; 4389 m deep; Challenger Expedition, Stn. 346; NHMUK 1890.5.7.1077. • 1 specimen, syntype of Freyella tuberculata; between Canary Islands and Cape Verde Islands; 2.7°S, 14.6833°W; 4298 m deep; Challenger Expedition, Stn. 89; NHMUK 1890.5.7.1076.

Description

Single specimen, with very small disc and six long, slender, tapered arms (R = 190 mm, r = 5 mm; Fig. 26A). Specimen before preservation has a slightly orange adoral disc surface, white arms, and bright orange tube feet discs (Fig. 26A–D). Disc is somewhat rounded, covered with short, scattered spines covered by a membrane bearing pedicellariae (Fig. 26B). Arms with long, slender lateral spines, also covered with a membrane bearing pedicellariae (Fig. 26C). Each abactinal plate on the genital area bears a one to few spinelets, completely covered with a membrane with pedicellariae. Each mouth plate has two oral spines covered by a clear membrane bearing pedicellariae; one located on the adoral margin of the mouth plate and the suboral spine located above the centre of the mouth plate.

Figure 26. 

Freyastera stet. CCZ_201 A in situ image B whole specimen C dorsal disc surface D ventral disc surface. Scale bars: 2 cm (A); 2 mm (B); 1 cm (C); 2 mm (D). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B–D).

Remarks

The COI sequence is 4% divergent from the two specimens of Freyastera cf. tuberculata reported herein, and hence considered a separate species. It is also divergent (> 4% K2P distance) to sequences of other species of Freyastera, but forms a monophyletic clade with those, confirming its placement within the genus (Fig. 24). Morphologically it resembles Freyastera cf. tuberculata, but differ in having slightly shorter and more scattered spinelets on the abactinal surface of the disc. Also, the spinelets on the abactinal plates on the genital area are more numerous, and completely covered by a membrane bearing pedicellariae, instead of having a membrane that does not cover the spine all the way down to the base as in F. cf. tuberculata. In addition, the genetic distance with specimens of that species corresponds to the genetic distance between morphologically distinct species and hence considered a separate species.

Ecology

The specimen was collected on the sedimented abyssal plain of APEI 1 at 5204 m depth, with arms curled up like a basket (Fig. 26A).

Comparison with image-based catalogue

Freyastera spp. are commonly found in image-based megafauna assessments across the CCZ (e.g., Amon et al. 2016; Amon et al. 2017b), abyssal areas of the Kiribati EEZ, and other areas of the Pacific abyss (e.g., Peru Basin: Simon-Lledó et al. 2019a), both in nodule fields and in seamount areas. The relatively large size of adult specimens facilitates the detection of these brisingids even upon imagery collected at high altitudes (> 5 m) above the seabed. However, only one Freyastera sp. morphotype (e.g., Freyastera sp. indet., AST_002) has been catalogued so far, as differences in structure of the abactinal armament and/or the suboral spines are not visible from seabed images.

Order Forcipulatida Perrier, 1884

Family Zoroasteridae Sladen, 1889

Genus Zoroaster Wyville Thomson, 1873

Zoroaster stet. CCZ_065

Fig. 27

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8877°N, 141.7569°W; 3132 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400691 (COI), ON406607 (16S); NHMUK 2022.78; Voucher code: CCZ_065.

Description

Single specimen (R = 16.6 cm, r = 1.3 cm). Actinal and abactinal surfaces are bright orange when alive, with ambulacrum slightly darker orange (Fig. 27A–D). Small disc; with five long, slender arms, gradually tapering distally (Fig. 27B). Carinal plates bear conical primary spines, forming a single longitudinal row that runs along the arm (Fig. 27A, C).

Figure 27. 

Zoroaster stet. CCZ_065 A in situ image B abactinal view of whole specimen C detail of abactinal surface D detail of actinal surface before preservation. Scale bars: 3 cm (A); 2 cm (B); 5 mm (C, D). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B–D).

Remarks

Morphological characters are concordant with the description of the genus Zoroaster. The phylogenetic analyses also recovered the specimen in a well-supported clade with other species of the genus (Fig. 24). However, COI divergence between species in the genus is very low (K2P distance ≈ 1–2%) and species-level clades were not recovered in the phylogeny, preventing us from assigning it to any species based on COI sequences. The closest match to the COI sequence of the CCZ specimen is a sequence from the long-armed morphotype (K2P distance 0.6%, GenBank accession number AY225785.1) identified for Z. fulgens Wyville Thomson, 1873 in the Porcupine Seabight, Atlantic Ocean (50.1987°N, 14.6593°W; 4001 m depth; Howell et al. 2004). Although this value is concordant with intraspecific divergence in the genus, the 16S sequences divergence between the CCZ specimen and the long-armed morphotype (K2P 1.1%) is larger than between the long-armed morphotype and Z. spinulosus Fisher, 1906 (K2P 0.0%) and between Z. spinulosus and Z. ophiactis Fisher, 1916 (K2P 0.3%).

Ecology

The specimen was found partially buried in the sediment on the seamount on APEI 7 at 3133 m depth.

Comparison with image-based catalogue

No similar Zoroasteridae morphotypes have been catalogued so far from seabed imagery in the eastern CCZ nor in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Zoroaster stet. CCZ_065 was catalogued as a new morphotype (i.e., Zoroaster sp. indet., AST_025).

Class Crinoidea

To date, there are 66 records of crinoids occurring deeper than 3000 m in the CCZ, with only seven of these representing preserved specimens (OBIS 2022). Three specimens, belonging to two species, were collected in the western CCZ. The barcoding gene COI was amplified for all specimens, and sequences were included in a concatenated alignment (16S, 18S, 28S, COI, and CytB) used to estimate a phylogenetic tree for the class (Fig. 28).

Figure 28. 

Rooted Bayesian phylogeny of Crinoidea. Concatenated (16S, 18S, 28S, COI, and CytB) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.

Subclass Articulata Zittel, 1879

Order Comatulida

Suborder Bourgueticrinina Sieverts-Doreck, 1953

Family Phrynocrinidae AH Clark, 1907

Subfamily Porphyrocrininae AM Clark, 1973

Genus Porphyrocrinus Gislén, 1925

Porphyrocrinus sp. CCZ_165

Fig. 29

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 6.9879°N, 149.9327°W; 5002 m deep; 06 Jun. 2018; Smith & Durden leg.; GenBank: ON400718 (COI), ON406616 (16S); NHMUK 2022.76; Voucher code: CCZ_165.

Description

Single specimen, attached to a nodule by a xenomorphic stalk (Fig. 29A). Crown (Fig. 29C) detached from stalk (Fig, 29B); L = 32 mm, composed of a crown and short proximal part of stalk. Proximal stalk composed of 5 very thin discoidal columnals up to 0.54 mm in diameter. Basal circlet truncated conical with distal diameter 0.54 mm and adoral diameter 0.78 mm; basals five, pentagonal in shape, with sunken lateral edges. Marked angle (~ 120°) between basals and radials. Radials five, pentagonal in shape; distal diameter 1.48 mm. Crown has five undivided arms. IBr1 are in close apposition with thin lateral flanges. Brachial formula 1+2 3+4 5+6 7+8 8+9 etc. First pinnule at IBr6; following pinnules every second ossicle; P1 has eight segments 4.04 mm in length; P2 is similar with eight pinnulars and 4.9 mm in length; P1 and P2 display lateral discoidal plates along ambulacral groove.

Figure 29. 

cf. sp. CCZ_165 A in situ image B xenomorph stalk attached to a polymetallic nodule C detached crown before preservation. Scale bars:1 cm (A). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B, C).

Remarks

Morphological characters are concordant with those of the family Phrynocrinidae and the genus Porphyrocrinus as understood by Messing (2016). This is the first record of the genus in the Eastern Pacific. Only two specimens have been previously recorded from similar depths but collected from the Eastern Atlantic and attributed to Porphyrocrinus cf. incrassatus (Eléaume et al. 2012). In the phylogenetic tree the specimen is recovered in a monophyletic clade with other sequences from members of the family (Fig. 28) and represents a new species. Based on genetic divergence of the COI gene (0.5% K2P), the specimen found in the eastern CCZ (Crinoidea sp. NHM_055; Glover et al. 2016b) belongs to the same species. However, the specimen in Glover et al. (2016b) was not identified to family level or lower taxonomic level due to its early developmental stage, lacking key diagnostic morphological features.

Ecology

The specimen was found attached to a nodule in the abyssal sediments of APEI 4 at 5001 m depth.

Comparison with image-based catalogue

No similar Comatulida morphotypes have been catalogued so far from seabed imagery in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of CCZ_065 was catalogued as a new morphotype (i.e., Porphyrocrinus sp. indet., CRI_008). Note however, that the in situ image of CCZ_065 was collected from an oblique angle and zoomed-in camera, generating a detailed view of a specimen that, owing to its small size, would be otherwise difficult to identify in quantitative assessments, e.g., where images are usually collected vertically-facing, fully zoomed out, and at a higher altitude above the seabed.

Superfamily Antedonoidea Norman, 1865

Family Antedonidae Norman, 1865

Subfamily Bathymetrinae AH Clark, 1909

Bathymetrinae inc. CCZ_176

Fig. 30

Material. Clarion-Clipperton Zone • 1 adult specimen; APEI 4; 6.9879°N, 149.9326°W; 5009 m deep; 06 Jun. 2018; Smith & Durden leg.; GenBank: ON400719 (COI), ON406617 (16S); NHMUK 2022.77; Voucher code: CCZ_176. • 1 specimen pentacrinoid stage; APEI 1; 11.2751°N, 153.7444°W; 5241 m deep; 09 Jun. 2018; Smith & Durden leg.; GenBank: ON400723 (COI), ON406618 (16S); NHMUK 2022.60; Voucher code: CCZ_186.

Description. Two specimens, one adult (CCZ_176; Fig. 30A, B) and one pentacrinoid stage (CCZ_186; Fig. 30C, D), both whitish when alive and attached to a glass sponge stalk. Adult with 10 arms; centrodorsal low conical. Cirri ~ 17, length 7.5 mm; c1 W > L; c2 W = L; c3 longest L = 0.95 mm, W at centre of ossicle = 0,25, W distal = 0,45; following cirrals decreasing in length to c8 or c9; c3 to c17 with everted distal edge; c8 to c14 with a spine on distal edge; c17 slightly longer than wide; claw same length as c17; opposing spine small. No basal visible. Radial 5, visible, extending beyond the rim of centrodorsal. First brachitaxis of two ossicles well separated laterally. Ibr1 rectangular slightly incised by Ibr2; Ibr2 axillery, losangic. Subsequent brachials very long; syzygies at 3+4, 9+10. First pinnule P1 on br2, 14 segments very thin and slender, composed of very long segments starting at p3 with L < 6× W. In pentacrinoid stage, five arms are visible, with orals, and stalk.

Figure 30. 

Bathymetrinae inc. CCZ_176 A side view of adult specimen. Specimen CCZ_186 B pentacrinoid stage attached to a glass sponge stalk C pentacrinoid stage. Scale bars: 5 mm (A); 1 mm (B); 0.5 mm (C). Image attribution: Wiklund, Durden, Drennan, and McQuaid (A, B); Bribiesca-Contreras 2019 (C).

Remarks. Morphological characters are concordant with those of the subfamily Bathymetrinae in the family Antedonidae. The closest match (2.7% K2P) to the COI sequences is a sequence of Psathyrometra fragilis (AH Clark, 1907) from Rodriguez Seamount (1887 m; SIO-BIC E4433), within the family Zenometridae. However, in the phylogenetic analysis the specimens were recovered in a different clade from Psathyrometra spp. (Fig. 28), but in a well-supported clade with two species (Crinoidea sp. NHM_056, Crinoidea sp. NHM_300) from the eastern CCZ (Glover et al. 2016b). The two species previously recorded in the eastern CCZ were delimited only from genetic sequences, as they seem to be early pentacrinoid stages and thus lack morphological characters for identification. However, based on the genetic divergence values with the species Bathymetrinae inc. CCZ_176 (~ 10% K2P), the two eastern CCZ species are most likely members of the subfamily Bathymetrinae.

Ecology. The adult specimen was found attached to a glass sponge stalk (Fig. 11A), along with an anemone, in abyssal sediments of APEI 4 at 5001 m depth. After careful examination of the material in the laboratory, a pentacrinoid stage was found attached to a sponge stalk (Figs 6A, 30B), along with the cirriped Catherinum cf. novaezelandiae (specimen CCZ_185) and the anemone Metridioidea stet. CCZ_164, in abyssal sediments of APEI 1 at 5241 m depth.

Comparison with image-based catalogue. A very similar Comatulida morphotype (i.e., Bathymetrinae gen. indet., CRI_001) has been commonly encountered in seabed image surveys conducted across the eastern CCZ, both in nodule fields and in seamount areas (Amon et al. 2017b). In contrast, CRI_001 was not encountered in image surveys conducted within abyssal areas of the Kiribati EEZ, where the presence of Crinoids was substantially lower than at the eastern CCZ (e.g., only nine specimens representing three morphotypes encountered in ~ 15,000 m2 of seabed surveyed; Simon-Lledó et al. 2019d).

Class Echinoidea

To date, there are 1455 records of echinoids occurring deeper than 3000 m in the CCZ, 11 of these representing preserved specimens (OBIS 2022). Two specimens belonging to different species were collected. Sequences for the barcoding gene COI were successfully amplified for both specimens and included in a COI-only phylogenetic tree.

Figure 31. 

Phylogenetic tree of Echinoidea. COI-only median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.

Subclass Euechinoidea Bronn, 1860

Infraclass Audolonta Jackson, 1912

Superoder Echinothuriacea Jensen, 1982

Order Aspidodiadematoida Kroh & Smith, 2010

Family Aspidodiadematidae Duncan, 1889

Genus Plesiodiadema Pomel, 1883

Plesiodiadema cf. globulosum (A. Agassiz, 1898)

Fig. 32

Material

Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2527°N, 153.5848°W; 5204 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON400726 (COI), ON406628 (18S); CASIZ 229305; Voucher code: CCZ_196.

Description

Single specimen, with a somewhat spherical, slightly flattened test (d = 2 cm, H = 1.5 cm). In situ colouration is purple, but the inflated anal cone is greyish blue (Fig. 32A). Primary spines are also purple, very long (up to 17 cm), thin, flexible, and strongly verticillate (Fig. 32B, C). Pedicellariae are tridentate (Fig. 32C).

Figure 32. 

Plesiodiadema cf. globulosum (A. Agassiz, 1898). Specimen CCZ_196: A in situ image B specimen after recovery C detail of pedicellaria of specimen CCZ_196. Scale bars: 2 cm (A); 1 cm (B). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B, C).

Remarks

In 1980, the RV Governor Ray collected several Aspidodiadematidae specimens in the CCZ at ~ 4,800 m, and were assigned to the species P. globulosum. The type localities of P. globulosum are the north of Malpelo Island, and from off Galera Point, Ecuador in the Pacific Ocean, from 2877 to 3241 m depth (Agassiz 1898). There are no genetic sequences available on public databases for the genus, but both COI and 18S closest matches are to species of the genus Aspidodiadema A. Agassiz, 1879, within the same family (18S: 99.4% similar to A. jacobyi A. Agassiz, 1880). The COI-only tree recovered a monophyletic clade including three specimens of A. tonsum (Fig. 31), but the genetic divergence is within interspecific values for COI (6.5–11.7%). Despite morphological characters being in accordance with the diagnostic characters for P. globulosum, the specimen is listed as cf. as the collection site is much deeper than the type locality.

Ecology

The specimen was collected on the sedimented abyssal plain of APEI 1, at 5203 m depth.

Comparison with image-based catalogue

A very similar Plesiodiadema sp. morphotype (i.e., Plesiodiadema globulosum sp. inc., URC_003) has been commonly found in image-based megafauna assessments conducted in the eastern CCZ (e.g., Amon et al. 2017b) and other areas of the eastern Pacific abyss (e.g., Yuzhmorgeologiya exploration area; Kamenskaya et al. 2013; Peru Basin; Simon-Lledó et al. 2019a), both in nodule fields and in seamount areas. URC_003 is usually the most abundant echinoid encountered in image-based megafauna surveys conducted at the eastern CCZ. In contrast, URC_003 was not encountered in surveys conducted in abyssal areas of the Kiribati EEZ, where kamptosomatids (e.g., see below) appeared to dominate the echinoid community (Simon-Lledó et al. 2019d).

Order Echinothurioida Claus, 1880

Family Kamptosomatidae Mortensen, 1934

Genus Kamptosoma Mortensen, 1903

Kamptosoma abyssale Mironov, 1971

Fig. 33

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.036°N, 149.9395°W; 5040 m deep; 01 Jun. 2018; Smith & Durden leg.; GenBank: ON400701 (COI); CASIZ 229306; Voucher code: CCZ_082.

Other material

Pacific Ocean • 1 specimen, holotype of Kamptosoma asterias (A. Agassiz); off the coast of Chile; 33.5167°S, 74.7167°W; 3950 m deep; Challenger Expedition, Stn. 299; NHMUK 1881.11.22.114. • 2 specimens, Kamptosoma abyssale Mironov, 1971; Tasman Sea; 0°N, 0°E; 4850–4800 m deep; Galathea Expedition, Stn. 574; NHMUK 1984.1.25.86-87.

Description

Single specimen (d = 3.4 cm, H = 1.6 cm). In situ, the body is reddish brown, rounded and flattened (Fig. 33A, B). Spines are the same reddish brown colour of the body; oral primary spines are encased by a fleshy, clear sac, swollen and brighter at the tip. The test and covering skin are very thin and gonads are visible through; primary spines are projected upwards and tube feet extending downwards from the lower half of the body. Whole abactinal surface (ambulacral and interambulacral) covered by primaries arranged in irregular lines along the median lines of the plates, with few secondaries or militaries near ambitus (Fig. 33C). Claviform (globiferous) pedicellariae carries two saccules and two valves. Before preservation, colouration was bright orange.

Figure 33. 

Kamptosoma abyssale Mironov, 1971. Specimen CCZ_082: A, B in situ images C oral view D aboral view of specimen before preservation. Scale bars: 1 cm (A, B); 5 mm (C, D). Image attribution: Durden and Smith (A, B); Wiklund, Durden, Drennan, and McQuaid (C, D).

Remarks

Only two species of Kamptosoma have been described to date. Kamptosoma asterias (Agassiz, 1881) was first described from off the coast of Chile at 3950 m depth (type locality: H.M.S Challenger St. 299), and from the east of Malden Island, Central Pacific, at 4750 m depth (type locality for K. indistinctum synonymous with K. asterias: H.M.S. Challenger St. 272) (Agassiz 1881; 1904). It has also been reported for the central Pacific Ocean, the Tasman Sea, Chile, Antarctica, and the southern Indian Ocean from 3890–4950 m depth (Anderson 2016). Kamptosoma abyssale type locality is the Kuril-Kamchatka Trench, from 6090–6235 m (Mironov 1971), and occurs in the Northwest Pacific, from Aleutian Islands to Kermadec Trench, and from Madagascar to east of Hawaii between 4374–6235 m depth (Mooi et al. 2004). These two species are only differentiated by the shape of the claviform pedicellaria (previously referred to as globiferous pedicellaria, but Mironov et al. (2015) suggested these to belong to the claviform group as their rudimentary valves are not functional), with two valves in K. abyssale, and three valves in K. asterias. The specimen from the CCZ has pedicellariae with two saccules and two valves, as described for K. abyssale. However, the COI sequence is very similar (0.47–1.4% K2P distance) to sequences of K. asterias collected in the Tasman Sea from 4570–4744 m depth, and are recovered in a well-supported clade (Fig. 31). These values of genetic divergence are within the intraspecific divergence that has been reported for echinoids (Chow et al. 2016), and therefore might belong to the same species. Nonetheless these specimens identified as K. asterias were reported to have claviform pedicellariae with two valves as described for K. abyssale (Anderson 2016). It has been suggested that K. abyssale is a synonym of K. asterias, and the former species will only be validated once material from both type localities is examined in detail (Anderson 2016; Mironov et al. 2015; Mooi et al. 2004). Kamptosoma asterias from the Central Pacific (St. 272) has been re-examined and was reported to have pedicellariae with three valves (Mironov et al. 2015). The holotype from St. 299 was examined in this study. Unfortunately, most pedicellariae have been lost and only a single claviform pedicellariae was found, this with two valves.

Ecology

The specimen was found crawling rapidly across abyssal sediment in APEI 4, at 5040 m depth. This morphotype has an unusually high crawling speed.

Comparison with image-based catalogue

A very similar Kamptosoma sp. morphotype (i.e., Kamptosoma abyssale sp. inc., URC_010) has been encountered in seabed image surveys conducted in abyssal areas of Kiribati’s EEZ, but not in the eastern CCZ. URC_010 was the most abundant echinoid morphotype encountered in the abyssal areas explored within Kiribati’s EEZ (Simon-Lledó et al. 2019d).

Class Holothuroidea

Holothurians are important components of the benthic deep-sea megafauna, and currently there are 367 records at a minimum depth of 3000 m in the CCZ, with 141 representing preserved specimens (OBIS 2022). Holothurians are amongst the most diverse invertebrate megafaunal taxa in the CCZ seafloor; a total of 106 different holothurian morphotypes has been so far catalogued in the image-based assessments consulted for this study, across the CCZ and nearby locations. We collected 18 specimens belonging to 15 different species, for which the COI gene was successfully amplified for all but one specimen. The gene 18S was successfully amplified for that specimen, as well as for other three. These were included in a concatenated alignment (12S, 16S, 18S, 28S, COI, and H3) used to estimate a phylogenetic tree (Fig. 34).

Figure 34. 

Phylogenetic tree of the class Holothuroidea. Concatenated (12S, 16S, 18S, 28S, COI, and H3) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.

Subclass Actinopoda Ludwig, 1891

Order Persiculida Miller, Kerr, Paulay, Reich, Wilson, Carvajal & Rouse, 2017

Family Molpadiodemidae Miller, Kerr, Paulay, Reich, Wilson, Carvajal & Rouse, 2017

Genus Molpadiodemas Heding, 1935

Molpadiodemas stet. CCZ_102

Fig. 35

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.2701°N, 149.7827°W; 3552 m deep; 03 Jun. 2018; Smith & Durden leg.; GenBank: ON400708 (COI); NHMUK 2022.66; Voucher code: CCZ_102.

Description

Single specimen, ~ 32 cm long (Fig. 35A). Body subcylindrical when alive, dorso-ventrally flattened in preserved specimen (L = 22 cm, W = 9 cm; Fig. 35E, F), tapering distally; body wall is completely covered in sediment and globigerinas, firm, wrinkly, with transverse folds and ridges giving a partly serrated appearance to the margin; brim present; anus and mouth ventral (Fig. 35D, E). Tube feet only visible on the ventral surface, cylindrical, and orange (Fig. 35C). Dorsal surface is whitish and ventral is yellowish in preserved specimen, but heavily covered by sediment. Ossicles in tentacles; unbranched rods and branched rods, with branches intertwining at the ends creating irregular perforated mesh (Fig. 35B).

Figure 35. 

Molpadiodemas stet. CCZ_102 A in situ image B tentacle ossicles C tube feet D dorsal surface E ventral surface of specimen before preservation. Scale bars: 2 cm (A); 20 μm (B); 1 cm (D, E). Image attribution: Durden and Smith (A); Bribiesca-Contreras (B, C); Wiklund, Durden, Drennan, and McQuaid (D, E).

Remarks

COI sequence forms a clade with other species of Molpadiodemas including M. villosus (Théel, 1886), M. morbillus O’Loughlin & Ahearn, 2005, M. crinitus O’Loughlin & Ahearn, 2005 and M. involutus (Sluiter, 1901). Closest is to M. morbilus (K2P 3.7–3.9%), and in the phylogenetic tree it is recovered in a well-supported clade with other species within the genus (Fig. 34), but species were not separated within the genus. Three species within the genus Molpadiodemas have been previously reported in the CCZ: M. altanticus (R. Perrier, 1898), M. villosus and M. helios O’Loughlin & Ahearn, 2005, with the latter species being recently described from the CCZ and so far not reported elsewhere (O’Loughlin and Ahearn 2005). However, morphological characters of Molpadiodemas stet. CCZ_102 are not in accordance with the description of any of those three described species.

Ecology

This specimen was collected on the sediment seafloor of a seamount in APEI 4 at 3552 m depth.

Comparison with image-based catalogue

A very similar Molpadiodemidae morphotype (i.e., Molpadiodemas sp. indet., HOL_103) has been commonly encountered in seabed image surveys conducted across the eastern CCZ (e.g., Amon et al. 2017b) and in abyssal areas of the Kiribati EEZ, mostly in nodule field areas.

Molpadiodemas stet. CCZ_194

Fig. 36

Material

Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2517°N, 153.6055°W; 5205 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON400725 (COI); NHMUK 2022.71; Voucher code: CCZ_194.

Description

Single specimen (Fig. 36A). Colouration of live specimen is whitish yellow, with skin somewhat translucent (Fig. 36A, E). Body subcylindrical in live specimen, but dorso-ventrally flattened when preserved, tapering anteriorly, ~ 4× as long as wide (L = 25 cm, W = 8.2 cm); semi-translucent body wall, longitudinal muscles visible through it; colouration of preserved specimen is yellowish (Fig. 36E), darker on the ventral side (Fig. 36F). Ventral surface with small, black, unidentified epibionts embedded in the skin (Fig. 36C, D). Specimen barely covered by sediment. Ossicles in tentacles; unbranched rods with thick central swelling; and branched rods, often with branches intertwining at the ends creating an irregular perforated mesh (Fig. 36B).

Figure 36. 

Molpadiodemas stet. CCZ_194 A in situ image B tentacle ossicles C epibionts on ventral surface D detail of epibionts E dorsal surface F ventral view of specimen before preservation. Scale bars: 2 cm (A); 25 μm (B); 1 cm (E, F). Image attribution: Durden and Smith (A); Bribiesca-Contreras (B–D); Wiklund, Durden, Drennan, and McQuaid (E, F).

Remarks

The COI sequence of Molpadiodemas stet. CCZ_194 is similar to sequences of other species of Molpadiodemas, including M. villosus, M. morbillus, M. crinitus, M. involutus, and Molpadiodemas stet. CCZ_102. COI genetic divergence between both specimens collected in the CCZ is 6%, in accordance with values of genetic interspecific divergence for the genus. The specimen is recovered in a well-supported clade along with other members of the genus (Fig. 34), but species are not well delimited. As mentioned above, three species of Molpadiodemas have been previously reported in the CCZ (O’Loughlin and Ahearn 2005). The tentacle ossicles from specimen CCZ_194 are very similar to those of M. helios, but this latter species is distinguished by the prominent tube feet that are barely visible in our specimen.

Ecology

This specimen was found on the sedimented seafloor of an abyssal plain on APEI 1 at 5205 m depth.

Comparison with image-based catalogue

A very similar Molpadiodemas sp. morphotype (i.e., Molpadiodemas sp. indet., HOL_004) has been commonly encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ (e.g., Amon et al. 2017b), but not in abyssal areas surveyed within the Kiribati EEZ.

Order Synallactida Miller, Kerr, Paulay, Reich, Wilson, Carvajal & Rouse, 2017

Family Synallactidae Ludwig, 1894

Synallactidae stet. CCZ_061

Fig. 37

Material. Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8877°N, 141.7569°W; 3132 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400688 (COI), ON406640 (18S); NHMUK 2022.75; Voucher code: CCZ_061.

Description. Single specimen; description of external morphological features only from in situ image as the specimen was damaged during collection (Fig. 37A). Body semi-circular, with ventral surface flattened, tapering distally; very wide, widest part of body ~ 7 cm; anus posterodorsal; mouth anteroventral. Tegument seems thick. Ossicles present in tentacles, slightly curved rods, < 250 μm (Fig. 37B).

Figure 37. 

Synallactidae stet. CCZ_061 A in situ image B tentacle ossicles. Scale bars: 2 cm (A); 50 μm (B). Image attribution: Durden and Smith (A); Bribiesca-Contreras (B).

Remarks. There are no close matches to the COI sequence of Synallactidae stet. CCZ_061 in public databases. The closest match is to Synallactidae stet. CCZ_066 (14% K2P). Both specimens are recovered in a well-supported clade representing the family Synallactidae (Fig. 34). They are recovered very close to Paelopatides Théel, 1886, but COI divergence suggest they belong to different genera.

Comparison with image-based catalogue. No similar Synallactidae morphotypes have been so far catalogued from seabed imagery collected in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of Synallactidae stet. CCZ_061 was catalogued as a new morphotype (i.e., Synallactidae gen. indet., HOL_120).

Ecology. This specimen was collected on the sedimented seafloor of a seamount in APEI 7 at 3132 m depth.

Synallactidae stet. CCZ_066

Fig. 38

Material. Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8896°N, 141.75°W; 3095 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400692 (COI), ON406642 (18S); NHMUK 2022.63; Voucher code: CCZ_066.

Description. Single specimen, body semi-circular with ventral surface flattened; ~ 3× longer than wide (L = 21 cm, W = 6 cm; Fig. 38A). Mouth anteroventral, anus posterodorsal. Colouration in live specimen is bright red (Fig. 38D, E). Specimen severely damaged during collection, guts separated from skin. Tegument is thick and leathery, with wart-like protrusions on the dorsal surface, more evident on live specimen (Fig. 38A), and with a small, very short, triangular dorsal appendage. Brim evident on ventral surface. Small tube feet arranged in two irregular rows, one on each side of ventrum, running longitudinally. Tentacles 18. Ossicles present in dorsal skin (Fig. 38B) and tentacles (Fig. 38C).

Figure 38. 

Synallactidae stet. CCZ_066 A in situ image B ossicles on dorsal skin C tentacle ossicles D dorsal surface E ventral surface of specimen before preservation. Scale bars: 2 cm (A, D, E); 50 μm (B, C). Image attribution: Durden and Smith (A); Bribiesca-Contreras (B, C); Wiklund, Durden, Drennan, and McQuaid (D, E).

Remarks. There are no close matches to the COI sequence of Synallactidae stet. CCZ_066 in public databases. The closest match is to specimen Synallactidae stet. CCZ_061 (14% K2P). Both specimens are recovered in a well-supported clade representing the family Synallactidae (Fig. 34). They are recovered very close to Paelopatides Théel, 1886, but COI divergence suggest they belong to different genera.

Ecology. This specimen was collected on the sedimented seafloor of a seamount on APEI 7 at 3095 m depth.

Comparison with image-based catalogue. No similar Synallactidae morphotypes have been so far catalogued from seabed imagery collected in the eastern CCZ nor in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of CCZ_066 was catalogued as a new morphotype (i.e., Synallactidae gen. indet., HOL_121). The dorsal protrusions that differentiate HOL_121 from HOL_120 may not be clearly visible in vertically-facing seabed imagery, and hence these two taxa might only be classifiable into a single, generic morphotype (i.e., HOL_120) in quantitative analyses.

Genus Synallactes Ludwig, 1894

Synallactes stet. CCZ_153

Fig. 39

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 6.9704°N, 149.9426°W; 5009 m deep; 06 Jun. 2018; Smith & Durden leg.; GenBank: ON400714 (COI); NHMUK 2022.69; Voucher code: CCZ_153.

Description

Single specimen (Fig. 39A). Body cylindrical, white, ~ 4× as long as wide (L = 10 cm, W = 2.7 cm), flattened proximally and rounded distally; flattened ventral surface. Two rows, upper and lower, of lateral, small, conical, thin processes, similar to those around the proximal edge (Fig. 39D). There is a row of yellowish, very small, tube feet in the mid-ventral surface, along the odd ambulacrum (Fig. 39E). Skin firm but translucent. Colour on live and preserved specimen is white. Ossicles abundant on dorsal body wall, spatulated crosses only with a long spinous apophysis, end of arms spatulated with holes (Fig. 39B). Ventral ossicles also spatulated crosses with a long spinous apophysis, smaller, sometimes with more than four arms, also spatulated ends of arms with holes (Fig. 39C).

Figure 39. 

Synallactes stet. CCZ_153 A in situ image B ossicles from dorsal skin C ossicles from ventral skin D dorsal view of specimen before preservation, E ventral view. Scale bars: 2 cm (A); 50 μm (B, C); 5 mm (D, E). Image attribution: Durden and Smith (A); Bribiesca-Contreras (B, C); Wiklund, Durden, Drennan, and McQuaid (D, E).

Remarks

The closest matches for the barcoding gene COI sequence are published sequences from the genus Bathyplotes (89.9% similarity), also within the family Synallactidae. The sequence is distinct from the only sequence of Synallactes sp. (GenBank accession number: KX874365.1) included in the phylogeny (Fig. 34), and they were not recovered as a monophyletic group. The DeepCCZ specimen was recovered sister to species of Bathyplotes, with Synallactes sp. recovered separately from the other genera in the family Synallactidae, concordant with previous results (Miller et al. 2017). Despite this, the specimen was assigned to the genus Synallactes based on external morphological characters that are concordant with those described from the genus. Species of Synallactes have previously been reported in the CCZ: Synallactes profundus (Koehler & Vaney, 1905) and Synallactes aenigma Ludwig, 1894; the latter being associated with manganese substrates. External morphology does not resemble to S. profundus.

Ecology

This specimen was found on the sedimented seafloor of an abyssal plain on APEI 4 at 5008 m depth.

Comparison with image-based catalogue

A very similar Synallactidae morphotype (i.e., Synallactes sp. indet., HOL_007) has been commonly encountered in seabed image surveys conducted across nodule field areas of the eastern CCZ (e.g., Amon et al. 2017b), but not in abyssal areas of the Kiribati EEZ, where synallactid specimens were very rarely encountered.

Family Deimatidae Théel, 1882

Genus Oneirophanta Théel, 1879

Oneirophanta stet. CCZ_100

Fig. 40

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.2647°N, 149.774°W; 3550 m deep; 03 Jun. 2018; Smith & Durden leg.; GenBank: ON400706 (COI), ON406643 (18S), ON406620 (16S); NHMUK 2022.84; Voucher code: CCZ_100.

Description

Single specimen; colouration of live specimen is beige, spotted with light brown and yellow on dorsal surface (Fig. 40A, C, D), and lighter on ventral surface, with suckers on tube feet and tentacles being dark brown (Fig. 40D). Body cylindrical, ~ 33 cm long and 8.8 cm wide; mouth anteroventral, anus posteroventral. Tentacles partly retracted. Papillae arranged in one or two rows along the dorsal radii, and in a single row along the ventrolateral radii above the tube feet. Tube feet ~ 50 pairs, arranged in two or three rows on each ventrolateral ambulacrum; few tube feet located along mid-ventral ambulacrum, among them two tube feet, one placed approx. half the body length and the other approx. three quarter of the body length; and few smaller feet close to anus. Dorsal ossicles spatulated crosses, crosses with open ramifications, and small irregular perforated plates; ventral ossicles crosses with open ramifications of different stage of development.

Figure 40. 

Oneirophanta stet. CCZ_100 A dorsal view of specimen before preservation B in situ image C ventral view D dorsal ossicles E ventral ossicles. Scale bars: 2 cm (A, C); 5 cm (B); 200 μm (D); 100 μm (E). Image attribution: Wiklund, Durden, Drennan, and McQuaid (A, C); Durden and Smith (B); Kremenetskaia (D, E).

Remarks

Closest match for COI and 16S sequences is to Oneirophanta setigera (Ludwig, 1893) (86.7% and 96.3%, respectively). In the phylogenetic tree, it is recovered in a well-supported clade representing the family Deimatidae, including Oneirophanta (Fig. 34). According to the external morphology Oneirophanta sp. CCZ_100 differs from Oneirophanta mutabilis mutabilis Théel, 1879, O. mutabilis affinis Ludwig, 1893 and O. conservata Koehler & Vaney, 1905 in high number of tube feet arranged in two or three rows and by absence of large, perforated plates on dorsum. It differs from O. setigera in high number of tube feet arranged in two or three rows and by presence of small, perforated plates and bigger perforations on spatulated crosses.

Ecology

The specimen was found on the sediment seafloor of a seamount on APEI 4 at 3550 m depth.

Comparison with image-based catalogue

No exactly similar Deimatidae morphotypes have been so far catalogued from seabed imagery collected in the eastern CCZ nor in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of CCZ_100 was catalogued as a new morphotype (i.e., Oneirophanta sp. indet., HOL_063). However, HOL_063 could be potentially confused with a similar shaped Deimatidae morphotype (e.g., Deimatidae gen. indet., HOL_062; also beige, cylindrical, with conspicuous projections on the dorsal surface arranged in four rows) found in the eastern CCZ (e.g., Amon et al. 2017b), with more abundant -though slightly thinner- projections, that may be difficult to distinguish in vertically facing images.

Oneirophanta cf. mutabilis Théel, 1879

Fig. 41

Material

Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.252°N, 153.5847°W; 5203 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON400724 (COI), ON406629 (18S), ON406619 (16S); NHMUK 2021.20; Voucher code: CCZ_193.

Other material

Indian Ocean • 3 specimens, syntypes of Oneirophanta mutabilis Théel, 1879; Eastern Indian, Antarctic Basin; 53.9167° S, 108.5833° E; 3566 m deep; Challenger Expedition, Stn. 157; NHMUK 1883.6.18.33.

Description

Single specimen, body uniformly white (Fig. 41A). Body almost cylindrical, > 2× as long as wide (L = 16 cm; W = 6.9 cm), being of almost equal breadth throughout the whole length and tapering posteriorly; mouth anteroventral and anus posteroventral. Tentacles 20, small, with a lightly brown tip, each with a terminal part with 6–8 unbranched processes. Long, pointed processes, or different lengths, arranged in four distinct rows, two rows running along the dorsal ambulacra with eight processes on each row, and the longest being approx. half of the body length (Fig. 41C); the other two rows placed on the sides of the body, slightly above the ventral lateral ambulacra. Ventral ambulacra with 11 and 13 tube feet, arranged in two irregular rows; odd ambulacrum naked, except for two tube feet arranged in the posterior half of the body, and ten surrounding the anus; processes crowded anteriorly (Fig. 41D). Thin skin, translucent, but hard and brittle, with numerous small and large perforated plates, with the small ones bearing two or three spines near the centre, and the large ones ~ 30 spines; ossicles imbricated, almost forming a skeleton (Fig. 41B).

Figure 41. 

Oneirophanta cf. mutabilis Théel, 1879. Specimen CCZ_193 A in situ image B dorsal ossicles C dorsal view before preservation D ventral view. Scale bars: 200 μm (B); 1 cm (C, D). Image attribution: Durden and Smith (A); Bribiesca-Contreras (B); Wiklund, Durden, Drennan, and McQuaid (C, D).

Remarks

Sequences for the 18S, 16S, and COI genes were most similar to sequences from Oneirophanta setigera (99.07%, 95.6%, 88.51% similarity, respectively), followed by other species within the family Deimatidae (i.e., Orphnurgus glaber Walsh, 1891 and Deima validum Théel, 1879). The specimen was recovered in a well-supported clade including all members of Deimatidae (Fig. 34), closest to Oneirophanta sp. CCZ_100 (K2P distance: 11%). Calcareous ossicles are concordant with those in Oneirophanta mutabilis. This species was originally described west of the Crozet Islands (H.M.S. Challenger station 146: 46.7667°S, 45.5167°E) at 2514 m depth (Théel 1879) but has been further divided in two sub-species, O. mutabilis mutabilis and O. mutabilis affinis. The former has been reported to be cosmopolitan, while the later has been recorded from the tropical eastern Pacific. Further analyses will be required to determine the validity of the subspecies and if the CCZ specimen belongs to any of those. It differs from the original description of O. mutabilis (Théel, 1879) in having an irregular number of pedicels around the ventral surface, the pedicels around the anus arranged triangularly instead of a transversal row, as well as the arrangement of the processes on bivium and trivium. However, Théel (1879) mentioned that several of the specimens examined differed from the specimen described in the number of pedicels and the arrangement of processes, and further studies should clarify if these are indeed subspecies.

Ecology

The specimen was found on the sediment surface of an abyssal plain on APEI 1 at 5203 m depth.

Comparison with image-based catalogue

A very similar Oneirophanta sp. morphotype (i.e., Oneirophanta sp. indet., HOL_058) has been encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ (e.g., Amon et al. 2017b), but not in the abyssal areas surveyed at the Kiribati EEZ.

Order Elasipodida Théel, 1882

Family Psychropotidae Théel, 1882

Genus Psychropotes Théel, 1882

Psychropotes verrucicaudatus Xiao, Gong, Kou, Li, 2019

Fig. 42

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 6.9878°N, 149.9119°W; 4999 m deep; 02 Jun. 2018; Smith & Durden leg.; GenBank: ON400703 (COI); NHMUK 2021.19; Voucher code: CCZ_086.

Description

Single specimen, colouration in situ is violet (Fig. 42A, B). Body elongated and anteriorly depressed (L = 34.7 cm, W = 10.2 mm); with a broad brim. Short (approx. one twelfth of body length), conical, single-pointed, dorsal unpaired appendage, placed 2/5 of the body length from the posterior end (Fig. 42A–C). Dorsal skin, including the dorsal appendage, covered in warts (Fig. 42C, F). Each wart has an ossicle in the centre, a giant cross with a central apophysis and strongly curved arms, all visible through the skin (Fig. 42E, F). Dorsal skin also contains smaller crosses with spiny arms (Fig. 42E, F). Approximately 30 pairs of mid-ventral tube feet arranged in two rows along the mid-ventral ambulacrum, arranged very close together on the anterior two thirds of the body, and scattered after posteriorly, with the last pairs being very close together again. Colouration of preserved specimen is also purple, with slightly lighter ventrum.

Figure 42. 

Psychropotes verrucicaudatus Xiao, Gong, Kou, Li, 2019. Specimen CCZ_086: A, B in situ images C dorsal view of specimen before preservation D ventral view E dorsal ossicles F detail of warts and ossicles on dorsal body wall G mouth tentacles. Scale bars: 5 cm (B); 2 cm (C, D); 100 μm (E). Image attribution: Durden and Smith (A, B); Wiklund, Durden, Drennan, and McQuaid (C, D); Bribiesca-Contreras (E–G).

Remarks

COI sequence is very similar (K2P distance = 0.77%) to the holotype of P. verrucicaudatus, and they were recovered together in the phylogenetic tree (Fig. 34). This species was described from the Jiaolong seamount, in the South China Sea, western Pacific Ocean at 3615 m deep (Xiao et al. 2019). External morphological characters are in accordance with the original description.

Ecology

The specimen was found on the sedimented abyssal plain in APEI 4 at 4999 m depth.

Comparison with image-based catalogue

A very similar Psychropotes sp. morphotype (i.e., Psychropotes verrucicaudatus sp. inc., HOL_045) has been commonly encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ (e.g., Amon et al. 2017b), but not in the abyssal areas surveyed within the Kiribati EEZ.

Psychropotes dyscrita (Clark, 1920)

Fig. 43

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.0212°N, 149.9355°W; 5040 m deep; 02 Jun. 2018; Smith & Durden leg.; GenBank: ON400702 (COI); NHMUK 2022.83; Voucher code: CCZ_083.

Description

Single specimen, ~ 30 cm long (Fig. 43A). Colouration of live specimen is yellow (Fig. 43A, B), with reddish-light purple on ventral surface (Fig. 43C). Tentacles 18, also reddish-light purple. Long, dorsal appendage with round end, slightly longer than the total body length, and developed very close to the posterior end of the body.

Figure 43. 

Psychropotes dyscrita (Clark, 1920). Specimen CCZ_083: A, B in situ images C lateral view D ventral view. Scale bars: 5 cm (A, B); 2 cm (C, D). Image attribution: Durden and Smith (A, B); Wiklund, Durden, Drennan, and McQuaid (C, D).

Remarks

Gebruk et al. (2020) morphologically examined the specimen collected during the DeepCCZ and re-established the species Psychropotes dyscrita based on this specimen. The holotype was collected in Peru, at 5206 m depth, and the species is known from the Central Pacific Ocean at depths of 5040–5206 m (Gebruk et al. 2020). Psychropotes dyscrita and P. moskalevi Gebruk & Kremenetskaia, 2020 are the two only known yellow species for this genus and were recovered as sister species (Fig. 34). The COI sequence for the DeepCCZ specimen is 1.1 ± 0.4% divergent (K2P distance) from specimens of P. moskalevi. Although this value seems low, the COI gene seems to be more conserved in the genus Psychropotes (1.1–13.4%, mean = 6.5%), with < 2% interspecific divergence between some species pairs (P. dyscrita-P. moskalevi, P. moskalevi-P. raripes Ludwig, 1893).

Ecology

The specimen was found on the sediment seafloor of an abyssal plain in APEI 4 at 5040 m depth.

Comparison with image-based catalogue

A very similar Psychropotes sp. morphotype (i.e., Psychropotes sp. indet., HOL_047) has been encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ (e.g., Tilot 2006), and in the Kiribati EEZ, where this taxon was the most abundant holothurian encountered (Simon-Lledó et al. 2019d). In pioneer seabed image surveys conducted at the CCZ, prior to the re-establishment of the species (Gebruk et al. 2020), this morphotype was typically classified as P. longicauda.Based on seabed imagery (e.g., without analysis of ossicles), it is not possible to determine whether HOL_047 specimens are P. dyscrita or P. moskalevi.

Genus Benthodytes Théel, 1882

Benthodytes cf. sanguinolenta Théel, 1882

Fig. 44

Material

Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2953°N, 153.742°W; 5245 m deep; 09 Jun. 2018; Smith & Durden leg.; GenBank: ON400720 (COI); NHMUK 2022.70; Voucher code: CCZ_178.

Description

Single specimen (Fig. 44A). Colouration of live specimen is light pink dorsally (Fig. 44B), darker ventrally (Fig. 44C). Tentacles 18, yellow, digitiform. Numerous dorsal papillae scattered on dorsal. Brim wide. Tube feet in double rows along the mid-ventral ambulacrum, ~ 30 pairs, yellowish. Ossicles not found.

Figure 44. 

Benthodytes cf. sanguinolenta Théel, 1882. Specimen CCZ_178: A in situ image B dorsal view of specimen before preservation C ventral view. Scale bars: 2 cm (A); 1 cm (B, C). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B, C).

Remarks

The closest match for the COI sequence is a sequence from B. sanguinolenta (GenBank: HM196505.1; 93.54% similarity) from the Ross Sea, Antarctica. A genetic study revealed two separate clades within B. sanguinolenta (O’Loughlin et al. 2011): (1) specimens from northwest Australia, and (2) Ross Sea. None of the samples included in O’Loughlin et al. (2011) are from the type locality (34.1167° S 73.9399°’W, off Chile, Pacific Ocean; 4000 m), but they identified at least two separate genetic species. The COI sequence of the specimen collected in the CCZ forms a third clade within the B. sanguinolenta species complex (Fig. 34). Genetic divergence (K2P distance) between the CCZ specimens and both NW Australia and Ross Sea clades is 10.1% and 7.3%, respectively, corresponding to values of intraspecific divergence in the group. In original description, Théel (1882) describes the body to be 6–7× longer than wide, whereas the preserved specimen collected in the CCZ is only ~ 3× longer than wide, but might be due to preservation as in in situ images it appears longer. However, the number of digitiform tentacles and appearance of small processes are concordant with the description of B. sanguinolenta. The sequence of Benthodytes cf. sanguinolenta from Glover et al. (2016b) does not form a clade with the CCZ specimen, with COI genetic distance being large (K2P 23%).

Ecology

The specimen was found on the sedimented seafloor of an abyssal plain in APEI 1 at 5249 m depth.

Comparison with image-based catalogue

No exactly similar Benthodytes sp. morphotypes have been so far catalogued from seabed imagery collected in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of specimen CCZ_178 was catalogued as a new morphotype (i.e., Benthodytes sanguinolenta sp. inc., HOL_124).

Benthodytes marianensis Li, Xiao, Zhang & Zhang, 2018

Fig. 45

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 5.1043°N, 141.8865°W; 4861 m deep; 25 May. 2018; Smith & Durden leg.; GenBank: ON400682 (COI); NHMUK 2022.82; Voucher code: CCZ_019.

Description

Single specimen (Fig. 45). Body is elongated, ~ 49.4 cm, dorso-ventrally flattened with flat ventral surface and inflated dorsal surface; anteriorly depressed and tapering posteriorly; colouration in live specimen is dark violet. Two irregular rows of large conical papillae running along the paired dorsal ambulacra.

Figure 45. 

Benthodytes marianensis Li, Xiao, Zhang & Zhang, 2018. CCZ_019: A in situ image B dorsal ossicles including peculiar cross-shaped ossicle C ventral ossicles. Scale bar: 5 cm (A); 100 µm (B, C). Image attribution: Durden and Smith (A), Kremenetskaia (B, C).

Remarks

The COI sequence is identical to the holotype of B. marianensis (K2P genetic distance = 0%) collected in the Mariana Trench at 5567 m depth (Li et al. 2018). These two sequences are also recovered together in the phylogenetic tree (Fig. 34). The species is only known from this location. Morphological characters are also concordant with the original description, including an uncommon, very peculiar, cross-shaped, dorsal ossicle (Fig. 45B).

Ecology

The specimen was found on the sedimented seafloor of an abyssal plain in APEI 7 at 4860 m depth.

Comparison with image-based catalogue

CCZ_019 resembles a Benthodytes sp. morphotype (i.e., Benthodytes sp. indet., HOL_111) encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ (Amon et al. 2017b) and the Kiribati EEZ. However, the vivid dark/violet colouration of HOL_011 (contrasting with background bright sediment) can constrain the visibility of papillae features in in situ photographed specimens, potentially making these hard to differentiate from other Benthodythes sp. morphotypes in vertically-facing seabed imagery.

Family Elpidiidae Théel, 1882

Genus Peniagone Théel, 1882

Peniagone leander Pawson & Foell, 1986

Fig. 46

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 5.1042°N, 141.8861°W; 4860 m deep; 25 May. 2018; Smith & Durden leg.; GenBank: ON400681 (COI), ON406621 (16S); NHMUK 2022.61; Voucher code: CCZ_018.

Description

Single specimen observed swimming (Fig. 46A). Specimen was severely damaged during collection, with only a few tentacles recovered, and hence description of morphological characters is based on in situ images. Body ovoid, slightly > 2× as long as it is wide. Velum composed of two pairs of fully fused papillae. Tube feet four pairs; three posteriormost pairs fused together forming a posterior swimming lobe; tube feet from the anteriormost pair very short.

Figure 46. 

Peniagone leander Pawson & Foell, 1986. Specimen CCZ_018: A, B in situ images C tentacle ossicles. Scale bars: 100 µm (C). Image attribution: Durden and Smith (A, B); Bribiesca-Contreras (C).

Remarks

The specimen collected during the DeepCCZ expedition was recovered in bits, so no morphological features can be distinguished. Only four reddish orange tentacles were recovered, which are embedded in a transparent skin where ossicles are evident. However, P. leander is one of the few species that can be identified from images. The external morphological characters evident in in situ images from the CCZ specimen are in accordance with the species description. The species was originally described from in situ images and video footage collected across the eastern CCZ (Pawson and Foell 1986) and subsequently observed in the area (e.g., Amon et al. 2017b).

In the phylogenetic tree, the CCZ specimen was recovered in a well-supported clade with other species of Peniagone (Fig. 34). It was recovered together with a sequence of P. leander, which was recently rediscovered and collected for the first time in the Mariana Trench (Gong et al. 2020), both close to P. diaphana as reported by Gong et al. (2020). The 16S sequence of the CCZ specimen is similar (K2P distance = 2%) to the only available sequence from P. leander, but no COI sequence was made available. Our COI sequence is > 12% divergent (K2P distance) from other species within the genus. The COI gene seems to be highly divergent between species in this genus. Using the data provided in Kremenetskaia et al. (2021) and including the CCZ sequence of P. leander, COI mean interspecific divergence in the genus is 15.9% (min = 2.5% and max = 22.7%), with our sequence of P. leander being 14.5%–21.2% divergent from other species within the genus. Intraspecific divergence for species in the genus was estimated between 0.9%–3.0%.

Ecology

The specimen was found swimming near the sediment surface on an abyssal plain in APEI 7 at 4860 m depth.

Comparison with image-based catalogue

Peniagone leander (HOL_028) has been commonly encountered in seabed image surveys conducted across the eastern CCZ (e.g., Amon et al. 2017b) and in abyssal areas of the Kiribati EEZ, usually swimming above the seabed but sometimes creeping on it. Body colour appears to be variable; bright red, semi-transparent, purplish, and whitish HOL_028 specimens have been encountered in seabed image surveys across the CCZ.

Peniagone vitrea Théel, 1882

Fig. 47

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 5.0442°N, 141.8164°W; 4875 m deep; 28 May. 2018; Smith & Durden leg.; GenBank: ON400699 (COI), ON406622 (16S); NHMUK 2022.64; Voucher code: CCZ_077.

Other material

Pacific Ocean • 1 specimen, syntype of Peniagone vitrea var. setosa Ludwig; South Pacific; 0.6°S, 86.7667°W; 2418 m deep; Albatross Expedition; NHMUK 1895.11.12.7. • 3 specimens, syntypes of Peniagone vitrea Théel, 1882; East of St. Paul, Indian-Antarctic Ridge; 42.7167°S, 82.1833°W; 2652 m deep; Challenger Expedition, Stn. 302; NHMUK 1883.6.18.82.

Description

Single specimen. Body long, ~ 3× as long as wide (Fig. 47C, D). Mouth anterior, downwards; foremost neck-like part bent forwards in acute angle with ventral surface (Fig. 47C); with ten tentacles of similar sizes; anus terminal. Velum consists of two pairs processes, fully fused by a membrane forming a lobe, with only the tips free; the two middle processes are much larger (Fig. 47A, B, D). Eight pairs of tube feet surrounding the posterior third of ventral surface, decreasing in size distally. Skin translucent in live specimen (Fig. 47A, B), but white, hard, and brittle after preservation, with numerous calcareous deposits (Fig. 47C, D). Dorsal ossicles with four spinose arms, slightly arched, with mostly two long spinose processes (Fig. 47E).

Figure 47. 

Peniagone vitrea Théel, 1882. Specimen CCZ_077: A, B in situ images C lateral view before preservation D dorsal view E dorsal ossicles. Scale bars:2 cm (A); 3 cm (B); 200 μm (E). Image attribution: Durden and Smith (A, B); Wiklund, Durden, Drennan, and McQuaid (C, D); Bribiesca-Contreras (E)

Remarks

Morphological external characters and ossicle morphology are in accordance with the original description of Peniagone vitrea. Unfortunately, no genetic sequences of P. vitrea are available in public databases. This species was described from off Patagonia at 2652 m depth. Using data from Kremenetskaia et al. (2021), the COI sequence of P. vitrea is 16.5%–18.8% divergent (K2P) from other species of Peniagone, and 17.9% divergent from the COI sequence of P. leander generated in this study. In the phylogenetic tree, it is recovered in a well-supported clade with other species of Peniagone (Fig. 34).

Ecology

The specimen was found feeding on the sedimented seafloor of an abyssal plain in APEI 7 at 4874 m.

Comparison with image-based catalogue

A very similar Peniagone sp. morphotype (i.e., Peniagone vitrea sp. inc., HOL_059) has been commonly encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ (e.g., Amon et al. 2017b), but not in the abyssal areas surveyed within the Kiribati EEZ.

Family Laetmogonidae Ekman, 1926

Genus Psychronaetes Pawson, 1983

Psychronaetes sp. CCZ_101

Fig. 48

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8877°N, 141.757°W; 3132 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400690 (COI), ON406630 (18S); NHMUK 2022.62; Voucher code: CCZ_063. • 1 specimen; APEI 4; 7.2647°N, 149.7741°W; 3562 m deep; 03 Jun. 2018; Smith & Durden leg.; GenBank: ON400707 (COI), ON406631 (18S); NHMUK 2022.65; Voucher code: CCZ_101. • 1 specimen; APEI 4; 7.2647°N, 149.7741°W; 3562 m deep; 03 Jun. 2018; Smith & Durden leg.; GenBank: ON400709 (COI), ON406639 (18S); NHMUK 2022.67; Voucher code: CCZ_103. • 1 specimen; APEI 4; 7.2647°N, 149.7741°W; 3562 m deep; 03 Jun. 2018; Smith & Durden leg.; GenBank: ON400710 (COI), ON406632 (18S); NHMUK 2022.68; Voucher code: CCZ_104.

Description

Four specimens (Fig. 48A–D). Body dorso-ventrally flattened (L = 15.9 cm, W = 3.4 cm), tapering at both ends; pronounced “neck” anteriorly; mouth anteroventral, anus posterodorsal. Tentacles 18, with long stems and large elongate oval discs (Fig. 48G). Body wall firm and leathery, dark violet in preserved specimen, with evident, numerous calcareous wheel-like ossicles that make the skin sparkle under light. Mid-ventral ambulacrum is naked; one irregular row of ≥ 40 tube feet running along each ventrolateral ambulacra (Fig. 48G), very conspicuous on live specimens (Fig. 48A–D), but fully retracted on preserved specimens. Paired dorsal ambulacra with ~ 20 papillae each; ~ 7 long thick papillae distributed along each ambulacrum, interspersed with smaller ones (Fig. 48A–E). There are ~ 18 large conical papillae surrounding the anterior margin dorsally, fully fused forming a fringe (Fig. 48B, D). Dorsal ossicles numerous, wheel-like, of different sizes (ranging from 77–340 μm in diameter) but mostly large; strongly concave; central primary cross with 4–6 struts, mostly four; smooth rim; with 10–16, mostly 12, short spokes (Fig. 48F, H).

Figure 48. 

Psychronaetes sp. CCZ_101. Specimen CCZ_063 A in situ image. Specimen CCZ_101 B in situ image F dorsal ossicles. Specimen CCZ_103 C in situ image H dorsal ossicles. Specimen CCZ_104 D in situ image E dorsal view of specimen before preservation G ventral view. Scale bars: 5 cm (A, D); 2 cm (B); 1 cm (E, G); 75 μm (F); 100 μm (H). Image attribution: Durden and Smith (A–D); Wiklund, Durden, Drennan, and McQuaid (E, G); Bribiesca-Contreras (F, H).

Remarks

Based on ossicle morphology, the four specimens were considered to belong to the same species. Sequence of the 18S were found to be identical between specimens CCZ_063, CCZ_101, and CCZ_104 (0.0% K2P distance) but 1.3% divergent from CCZ_103. The COI gene was amplified for the four specimens and genetic divergence ranges between 0.8% to 7.4%. The two specimens collected in APEI 4 are less genetically divergent (CCZ_101-CCZ_104 = 0.8% K2P). The specimen collected in APEI 7 (CCZ_063) is 2.3–2.9% divergent from the other three. The specimen CCZ_103 is 7.4% divergent to CCZ_101 and CCZ_104, but only 2.9% divergent from CCZ_063. While the former values are within the range of interspecific genetic divergence, we considered the specimen to belong to the same species as both the ossicle and external morphological characters are similar to the other three specimens. In addition, the trace files for both 18S and COI for this specimen are messy and the high genetic divergence could be an artifact of miss-called nucleotides. Unfortunately, there are no sequences available for Psychronaetes, but we included sequences of other genera within the family (Pannychia, Laetmogone, and Benthogone) for which COI genetic divergence ranged from 23–31%. In the phylogenetic tree, the four specimens were recovered in a well-supported clade (Fig. 34), close to other Laetmogonidae (poorly supported). This species has an anterior brim, which is characteristic of the monotypic genus Psychronaetes. Psychronaetes hanseni Pawson, 1983 differs from the four specimens in having smaller dorsal wheel ossicles (d = 50–80 µm) with 9–12 spokes, only 15 tube feet on each of paired ventral ambulacrum, 15 mouth tentacles instead of 18, and in the number of papillae on the dorsal paired ambulacra. The species and genus were described from two specimens collected in the CCZ (Pawson 1983).

Ecology

The four specimens were found on the sedimented seafloor of seamounts in APEIs 4 and 7 between 3132–3562 m depth.

Comparison with image-based catalogue

No exactly similar Psychronaetes sp. morphotypes have been encountered in seabed image surveys conducted in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ images of these specimens were catalogued as a new morphotype (i.e., Psychronaetes sp. indet., HOL_110). However, HOL_110 can resemble at least two other Laetmogonidae morphotypes catalogued from seabed imagery; Laetmogonidae gen. indet., HOL_030 (e.g., dark violet, but with 8+ long papillae) which is commonly found in the eastern CCZ (but not in the Kiribati EEZ); and Psychronaetes sp. indet., HOL_122 (e.g., violet, but only with six or seven long papillae and with fewer (< 20) and larger, thick tube feet) which was also only found in the western CCZ.

Genus Laetmogone Théel, 1879

Laetmogone cf. wyvillethomsoni Théel, 1979

Fig. 49

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.8877°N, 141.7569°W; 3132 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400689 (COI), ON406641 (18S); NHMUK 2021.18; Voucher code: CCZ_062.

Other material

Pacific Ocean • 1 specimen, holotype of Laetmogone spongiosa Théel, 1879; south of Japan; 34.1167°N, 138°E; 1033 m deep; Challenger Expedition, Stn. 235; NHMUK 1883.6.18.47.

Description

Single specimen (Fig. 49A). Body cylindrical, ~ 3× as long as wide (L = 15.6 cm, W = 5.2 cm), with convex dorsal surface and somewhat flattened ventral surface, tapering posteriorly; mouth anterior, subventral, terminal; anus posterior, terminal, slightly dorsal; violet colouration in live and preserved specimen, with darker ventral surface (Fig. 49A–C). Tentacles 15, of almost equal size, and very dark at the tips. Odd ambulacrum is naked; 27 or 28 tube feet arranged in a single row on each of the paired ventral ambulacra, forming a continuous line on the anterior ⅔ of the body and scattered posteriorly, also decreasing in size (Fig. 49C). Each paired dorsal ambulacrum with a single row of long processes, 12 on the left and 13 on the right; longest processes longer than ⅓ of the body length. Twenty pedicels along each side of the ventral surface, posterior pairs smaller than the others. Fourteen processes of the bivium along the left ambulacra and thirteen along the right. Tegument is thick, completely covered by calcareous ossicles. Dorsal ossicles are wheel-like of various sizes (40–226 μm in diameter), with four or five studs, mostly five, on primary central crosses, and with 8–17 spokes, mostly eight on large wheels; ossicle is convex, rim smooth, interspoke areas small, and large central area on large wheels (Fig. 49D).

Figure 49. 

Laetmogone cf. wyvillethomsoni Théel, 1979. Specimen CCZ_062 A in situ image; B dorsal view of specimen before preservation C ventral view D dorsal calcareous ossicles. Scale bars: 2 cm (A); 1 cm (B, C); 50 μm (D). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B, C); Bribiesca-Contreras (D).

Remarks

Closest match on public databases for the COI gene sequence was other sequences of Laetmogone wyvillethomsoni Théel, 1879 (4.0–5.8% K2P genetic distance) from the Ross Sea and Marie Byrd Seamounts (O’Loughlin et al. 2011). The specimen from the CCZ and specimens from L. wyvillethomsoni from Antarctica were recovered in our phylogeny (Fig. 34) in a well-supported clade (Fig. 34), which is subdivided in three clades including the two Antarctic clades stratified by depth reported in O’Loughlin et al. (2011), and the specimen from the CCZ. Type material for L. wyvillethomsoni was collected during the H.M.S. Challenger expedition at stations 300 (off the coast of South America; 33.7° S, 78.3°W; 2514 m depth) and 147 (west of the Crozet Islands; 46.2667° S, 48.45° E; 2926 m), and high morphological variability was reported (Théel 1879). The CCZ specimen morphologically resembles L. wyvillethomsoni, but no rod-shaped ossicles were found in the dorsal skin, differing from the original description.

Ecology

The specimen was found on the sedimented seafloor of a seamount in APEI 7 at 3132 m depth.

Comparison with image-based catalogue

No similar laetmogonid morphotypes have been encountered in seabed image surveys conducted in the eastern CCZ or in abyssal areas of the Kiribati EEZ. Consequently, the in situ image of specimen CCZ_062 was catalogued as a new morphotype (i.e., Laetmogone sp. indet., HOL_123).

Class Ophiuroidea

To date, there are 1201 records of ophiuroids occurring at > 3000 m depth in the CCZ, with 117 representing preserved specimens (OBIS 2022). Four specimens belonging to three different species were collected and the barcoding gene COI was amplified for all but one, for which both 18S and 28S were amplified. These sequences, excluding 18S, were included in a concatenated alignment (28S, and COI) and used to estimate a phylogenetic tree (Fig. 50). Ophiuroidea is amongst the most challenging groups to identify and classify based on seabed image data only; key morphological features are too small to be appropriately visualised (e.g., plates and scales) and/or are found on the ventral disc (not visible in images). As a result, the taxonomic resolution of ophiuroid morphotypes catalogued from seabed imagery is usually much lower than that in other echinoderm groups. Consequently, connectivity and distribution patterns of ophiuroids derived from seabed image data should be interpreted cautiously.

Figure 50. 

Phylogenetic tree of Ophiuroidea. Concatenated (28S, and COI) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.

Subclass Myophiuroidea Matsumoto, 1915

Infraclass Metophiurida Matsumoto, 1913

Superorder Ophintegrida O’Hara, Hugall, Thuy, Stöhr & Martynov, 2017

Order Ophioscolecida O’Hara, Hugall, Thuy, Stöhr & Martynov, 2017

Family Ophioscolecidae Lütken, 1869

Genus Ophiocymbium Lyman, 1880

Ophiocymbium tanyae Martynov, 2010

Fig. 51

Material

Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2523°N, 153.5848°W; 5204 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON406633 (18S), ON406596 (28S); NHMUK 2022.74; Voucher code: CCZ_206.

Description

Single specimen (disc diameter = 9 mm, maximum arm length = 25 mm). Disc subpentagonal, flattened (Fig. 51A, B). Dorsal disc surface covered with numerous, imbricated, delicate disc scales, which are irregular in shape, decrease in size distally and extend dorsally onto the first arm segments (Fig. 51C). Radial shields and genital plates apparently absent. Disc covered by thin skin, not obscuring the scales. Ventral surface of the disc covered by scales similar to the dorsal disc scales (Fig. 51D). Oral shield somewhat triangular, approx. as long as wide, with convex distal edge; separated from first lateral arm plate by the adoral shields. Adoral shields are wing-shaped, narrowing proximally. Each jaw bears a large, spiniform, apical papillae and a smaller adjacent one on each side; additionally, there are two to three modified papillae placed distally on each side of the jaws, block-shaped, the distalmost being wing-shaped. Genital slit is not conspicuous. Arms are thin, longer than twice the disc diameter (Fig. 51A, B). Dorsal arm plates are triangular, wider than long, with pointed proximal and straight distal edges; separated by lateral arm plates and therefore not overlapping with preceding dorsal arm plate (Fig. 51C). Arm spines are conical, tapering distally but with rounded tips; two arm spines on first three arm segments, three arm spines on next three segments and four on the rest; middle arm spine is the longest, but all are approx. the same length, approx. half the length of one arm segment. First ventral arm plate is triangular, while the rest are pole-axe shaped, approx. as long as wide, separated from the preceding plate by the lateral arm plates except for the first two ventral arm plates (Fig. 51D). Tentacle pores are large and evident throughout the entire length of the arm. Three flattened, rounded, large adoral shield papillae. First four arms segments with two large papilliform tentacle scales attached to the lateral arm plate; subsequent arm segment with a single tentacle scale; tentacle scales absent on the remaining arm segments.

Figure 51. 

Ophiocymbium tanyae Martynov, 2010 A dorsal view of specimen CCZ_206 before preservation B ventral view C detail of dorsal disc surface and dorsal arm plates D detail of jaws, ventral disc surface and ventral arm plates. Scale bars: 2 cm (A, B); 5 mm (C, D). Image attribution: Wiklund, Durden, Drennan, and McQuaid (A–D).

Remarks

Morphological characters of the specimen are in accordance with the description of O. tanyae, which was collected in the Izu-Bonin Trench at 6740–6850 m depth. It differs from the original description in having arms ≥ 2× as long as the disc diameter (dd), instead of being approx. the same. It also differs on the tentacle scales, which extend to the fifth segment, instead of just the third, having two tentacle scales in the first four segments instead of just one, and in the number of arm spines of the first arm segments. The number of arm spines is discussed to vary amongst the paratypes (Martynov 2010), and it is very likely that tentacle scales are easily lost and therefore the number could differ between specimens. Only 18S and 28S were amplified for this specimen. The 28S sequence of the CCZ specimen is identical (K2P = 0%) to the sequence of the species Ophioscolecidae sp. 20 recently reported for the CCZ (Christodoulou et al. 2020). Both specimens are recovered within the same clade, that includes other species of the order Ophioscolescida (Fig. 50). Ophioscolescida sp. 20, Ophiocymbium tanyae, and O. rarispinum Martynov, 2010 are recovered as a clade possibly representing the genus Ophiocymbium. The species from Christodoulou et al. (2020) was identified from DNA sequences only, as the four specimens collected (eastern IFREMER and APEI 3) are tiny juveniles with no distinctive morphological characters. The species is therefore distributed in the Izu-Bonin Trench and the Clarion-Clipperton Zone.

Ecology

The specimen was found on the sedimented seafloor of an abyssal plain on APEI 1 at 5204 m depth.

Ophiocymbium cf. rarispinum Martynov, 2010

Fig. 52

Material

Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2518°N, 153.6059°W; 5206 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON400727 (COI); NHMUK 2022.73; Voucher code: CCZ_197.

Description

Single specimen, with white arms and greyish blue disc in situ (Fig. 52A). Disc is flattened and somewhat pentagonal; brownish when alive and white after preservation (Fig. 52B, D). Dorsal disc surface is covered by minute, thin, imbricated scales covered by a thin skin, not obscuring the scale margins; few granuliform spinelets scattered on the dorsal surface (Fig. 52B). Small, oval, radial shields, approx. as long as wide, arranged diagonally and touching proximally; distal margin extends beyond the disc margin (Fig. 52D). Ventral surface of the disc covered by scales similar to the ones on the dorsal surface, but lacking spinelets; gonads are visible through the thin scales (Fig. 52C). Each jaw bears two or three apical papillae, and three oral papillae on each side; two distalmost are block-shaped while the third distalmost is spiniform. Oral shield is triangular, longer than wide, rounded proximally; separated from the first lateral arm plate by the wing-shaped adoral shield. Supplementary oral shield, wider than long, located on the distal margin of the oral shield. Two adoral shield papillae, with one placed in the middle of each shield, resembling arm spines in shape and size. Arms are slender, ≥ 3× as long as the disc diameter. Dorsal arm plates triangular, with rounded distal margin and slightly convex distal edge, separated from preceding plates by lateral arm plates; first two dorsal arm plates absent but arm segments covered by thick skin with smaller plates embedded (Fig. 52D). Adjacent lateral arm plates are slightly separated by soft tissue; each lateral arm plate bears four long arm spines, similar in size; only two arm spines present in the first three arm segments and three on subsequent two arm segments. First ventral arm plate is small, broad and triangular, with the rest being pole-axe shaped and separated from the preceding plate except for the first two segments. Tentacle pores are large throughout the entire length of the arm, with no tentacle scales except for the first arm segment, where there is one attached to the lateral arm plate and resembles a small arm spine.

Figure 52. 

Ophiocymbium cf. rarispinum Martynov, 2010. Specimen CCZ_197 A in situ image B dorsal surface before preservation C detail of ventral surface, jaws and ventral arm plates D detail of dorsal arm plates. Scale bars: 1 cm (A, B); 5 mm (C); 2.5 mm (D). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B–D).

Remarks

In the phylogenetic tree, the specimen from the western CCZ is recovered as closely related to Ophiocymbium tanyae (Fig. 50). Ophiocymbium rarispinum was described from the Izu-Bonin Trench, between 6740 and 6850 m depth (ZMMU D-798), and no genetic sequences have been published. Morphological characters are concordant with the description of O. rarispinum, but differs in the length of the arms, the number of oral papillae and the number of arm spines.

Ecology

The specimen was found crawling on the abyssal sediments of APEI 1 at 5206 m depth.

Comparison with image-based catalogue

A similar Ophiuroidea morphotype (i.e., Ophiocymbium sp. indet., OPH_013) has been encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ, but not in the abyssal areas surveyed within the Kiribati EEZ.

Superorder Euryophiurida O’Hara, Hugall, Thuy, Stöhr & Martynov, 2017

Order Ophiurida Müller & Troschel, 1840 sensu O’Hara et al. 2017

Suborder Ophiurina Müller & Troschel, 1840 sensu O’Hara et al. 2017

Family Ophiopyrgidae Perrier, 1893

Genus Ophiuroglypha Hertz, 1927

Ophiuroglypha cf. irrorata (Lyman, 1878)

Fig. 53

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 4.9081°N, 141.6813°W; 3239 m deep; 26 May. 2018; Smith & Durden leg.; GenBank: ON400685 (COI); NHMUK 2021.21; Voucher code CCZ_058. • 1 specimen; APEI 7; 4.8897°N, 141.75°W; 3096 m deep; 27 May. 2018; Smith & Durden leg.; GenBank: ON400686 (COI); NHMUK 2022.72; Voucher code: CCZ_059.

Description

Two specimens, with greyish disc and pale arms in situ (Fig. 53A, B). Disc rounded to pentagonal, flattened, with slender, long arms, at least disc diameter (disc diameter = 2.6 cm, arm length = 13.1 cm; Fig. 53C, D). Dorsal disc surface covered by irregular, larger disc scales surrounded by small, imbricated disc scales that also vary in size and shape. Radial shields are small, subtriangular, almost as wide as long. Arm combs visible under the radial shields; with five short block-like arm-comb spinelets, not continuous on dorsal midline. Ventral surface of the disc is covered by large, imbricated scales, increasing in size towards the margin of the disc (Fig. 53F). Oral plates with 6–8 oral papillae, proximalmost are pointed, becoming block-like towards the distal side of the oral plate. Oral shield approx. as long as wide, subpentagonal, with somewhat concave proximal margins, a convex distal margin, and with lateral margins slightly constricted in the middle, where the genital slit begins. Adoral shields touching proximally, with a similar width all along, and separating the oral shield from the first lateral arm plates. Genital slits run from the middle of the oral shield to the disc margin, bordered by a continuous row of block-like genital papillae that continues dorsally as an arm-comb.

Figure 53. 

Ophiuroglypha cf. irrorata (Lyman, 1878). Specimen CCZ_059 A in situ image. Specimen CCZ_058 B in situ image C dorsal view of specimen before preservation D ventral view E arm hooklets F detail of ventral disc surface and ventral arm plates. Scale bars: 2 cm (A, B); 1 cm (C, D); 2 mm (F). Image attribution: Durden and Smith (A, B); Wiklund, Durden, Drennan, and McQuaid (C–F).

Dorsal arm plates fan-shaped, contiguous. Lateral arm plates bear three short (less than a third of the length of the arm segment) arm spines from the third arm segment; two are located ventrally, very close together, and one located dorsally, approx. halfway through the lateral arm plate; first arm segment bears two arm spines, the second two or three spines. Ventral arm plate trapezoidal, wider than long, only touching the preceding plate only on first three arm segments, after which they are separated by the lateral arm plates and become fan-shaped to rhomboidal, more than twice as wide as long, with pointed proximal edge and rounded distal margin. Towards the distal end of the arms, the second lowest spine is modified into a hyaline hooklet (Fig. 53E). Tentacle pores only on most proximal segments (8–11), with six ventral and six lateral tentacle scales on first arm segment and decreasing in number until there is a single, very small, spiniform, tentacle scale remaining for most of the arm length.

Remarks

Both specimens collected are only 0.4% divergent (K2P distance) in COI sequences between them. Closest genetic match is Ophiuroglypha sp. (8% K2P distance) collected in the CCZ (Christodoulou et al. 2020), and in the phylogenetic tree they were recovered in a well-supported clade along with other species of Ophiuroglypha (Fig. 50). Both specimens have an upturned hook in the second lowest arm spine, which is characteristic of species of the genus Ophiuroglypha (previously a subgenus but raised to genus by O’Hara et al. (2018)). Morphologically, the species resembles to Ophiuroglypha irrorata concreta (Koehler, 1901) based on the arm spine arrangement, dorsalmost spine separated from the two ventral spines. However, the DeepCCZ specimens are listed as O. cf. irrorata, as a recent study suggested that the arm spine arrangement might not be species specific, hence questioning the validity of O. irrorata irrorata (Lyman, 1878) and O. irrorata concreta (Stöhr and O’Hara 2021). Additionally, molecular data has suggested that O. irrorata represents an unresolved complex of species (Christodoulou et al. 2019).

Ecology

Both specimens were found on the sedimented seafloor of a seamount in APEI 7, at 3096 (specimen CCZ_059) and 3239 m (specimen CCZ_058) depth.

Comparison with image-based catalogue

No similar Ophiuroidea morphotypes have been encountered in seabed image surveys conducted in the eastern CCZ nor in abyssal areas of the Kiribati EEZ. Consequently, the in situ images of CCZ_058 and CCZ_059 were catalogued as a new morphotype (i.e., Ophiuroglypha sp. indet., OPH_012).

Phylum Porifera Grant, 1836

A total of eight sponges was collected in the western CCZ. All these belong to the class Hexactinellida and represent seven different species, but none was confidently assigned to any known species. To date, there are 255 records of hexactinellid sponges occurring at > 3000 m depth in the CCZ, with only eight representing preserved specimens (OBIS 2022). Several genes were targeted for amplification, but only 16S was successfully amplified for all of them. Other genes amplified were COI (7 specimens), 18S (5), 28S (5), and ALG11 (3). Sequences of these genes were combined with the concatenated alignment from Dohrmann (2018), and the phylogenetic tree was estimated using the same parameters (Fig. 54).

Figure 54. 

Phylogenetic tree of Hexactinellida. Concatenated (16S, 18S, 28S, and COI) median consensus BEAST tree with posterior probability (PP) and bootstrap (BS) values indicated. Only values of PP > 0.70 and BS > 50 are shown, with values of PP > 0.95 and BS > 90 indicated with a circle. Nodes not recovered on the RAxML tree are indicated with a hyphen. Sequences generated in this study are highlighted in violet.

Class Hexactinellidae Schmidt, 1870

Subclass Amphidiscophora Schulze, 1886

Order Amphidiscosida Schrammen, 1924

Family Hyalonematidae Gray, 1857

Genus Hyalonema Gray, 1832

Hyalonema stet. CCZ_020

Fig. 55

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 5.1149°N, 141.8967°W; 4856 m deep; 25 May. 2018; Smith & Durden leg.; GenBank: ON400683 (COI), ON406634 (18S), ON406608 (16S), ON406597 (28S), ON411254 (ALG11); NHMUK; Voucher code: CCZ_020. • 1 specimen; APEI 1; 11.2954°N, 153.7422°W; 5245 m deep; 09 Jun. 2018; Smith & Durden leg.; GenBank: ON400721 (COI), ON406609 (16S); NHMUK 2022.8; Voucher code: CCZ_179.

Description

Two specimens. Lophophytous sponges (Fig. 55A–C). Body is white, ovoid, almost as wide as long (CCZ_020 W = 4.7 cm, L = 5 cm; CCZ_179 W = 8 cm, L = 8 cm); with an osculum (CCZ_020 d = 1 cm; CCZ_179 d = 4 cm) surrounded by a thin margin; separated atrial cavity. Attached to the sediment by a thin tuft of basalia that extends from the central lower body (CCZ_020 L > 7 cm; CCZ_179 L > 20 cm).

Figure 55. 

Hyalonema stet. CCZ_020. Specimen CCZ_179 A in situ image B specimen before preservation. Specimen CCZ_020 C in situ image. Scale bars: 2 cm (A, C); 1 cm (B). Image attribution: Durden and Smith (A, C); Wiklund, Durden, Drennan, and McQuaid (B).

Remarks

Genetic sequences between specimens CCZ_020 and CCZ_179 are 1% and 0.8% divergent (K2P distance) for COI and 16S, respectively. COI and 16S closest matches are sequences from Tabachnickia sp. within the Hyalonematidae. The sequence for the 18S is > 95% similar to other species of Hyalonema. In the phylogenetic tree, both specimens were recovered together, in a well-supported clade with other members from different subgenera within the genus Hyalonema and including Tabachnickia sp. (Fig. 54).

Ecology

The specimens were collected attached to abyssal sediments of APEI 7 and APEI 1 at 4856 and 5245 m depth, respectively.

Comparison with image-based catalogue

A very similar hyalonematid morphotype (i.e., Hyalonema sp. indet., HEX_002) has been commonly encountered in seabed image surveys conducted across the eastern CCZ and in abyssal areas of the Kiribati EEZ, mostly in nodule field areas. In situ images of HEX_002 (Fig. 55A, C) show that the aperture width of the central osculum is an unreliable character to distinguish different Hyalonema sp. morphotypes (nor these from other genera) in seabed imagery, as this contracts and expands episodically (e.g., Kahn et al. 2020).

Figure 56. 

Hyalonema stet. CCZ_081 A in situ image, B. Scale bars: 1 cm (A); 5 mm (B). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B).

Hyalonema stet. CCZ_081

Fig. 56

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 7.036°N, 149.9395°W; 5031 m deep; 01 Jun. 2018; Smith & Durden leg.; GenBank: ON406610 (16S); NHMUK 2022.9; Voucher code: CCZ_081.

Description

Single specimen (Fig. 56). Lophophytous sponge; body white, somewhat bowl-shaped, longer than wide (L = 3.9 cm, W = 2.8 cm); with a wide osculum (d = 2.6 cm) surrounded by a thin margin. Attached to the sediment by a thin tuft of basalia that extends from the central lower body.

Remarks

Morphological characters were found concordant with those of the genus Hyalonema. The 16S sequence is very similar (99.34%) to sequences from H. (Cyliconemaoida) ovuliferum Schulze, 1899, being the closest match on public databases. It is recovered in a well-supported clade along with other hyalonematids (Fig. 54), supporting its placement within the genus. It was not possible to assign it any subgenus, as the different subgenera have not been recovered as monophyletic (Dohrmann 2018).

Ecology

This specimen was collected anchored to the sediment on the abyssal plain of APEI 4 at 5031 m.

Comparison with image-based catalogue

A very similar hyalonematid morphotype (i.e., Hyalonema sp indet., HEX_003) has been commonly encountered in seabed image surveys conducted across the eastern CCZ and in abyssal areas of the Kiribati EEZ. As observed in HEX_002, the aperture width of the central osculum in HEX_003 can vary owing to body contractions or expansions (Kahn et al. 2020), and should hence not be used to guide identifications of these morphotypes based on seabed imagery.

Subclass Hexasterophora Schulze, 1886

Order Lyssacinosida Zittel, 1877

Family Euplectellidae Gray, 1867

Subfamily Euplectellinae Gray, 1867

Euplectellinae stet. CCZ_199

Fig. 57

Material. Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2518°N, 153.5853°W; 5202 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON400729 (COI), ON406611 (16S); NHMUK; Voucher code: CCZ_199.

Description. Single specimen (Fig. 57A). Lophophytous white sponge with tubular habitus (L = 5 cm). There is a large, central osculum (d = 2cm), and protruding fistules with terminal suboscula, each with one or two, mostly two, openings (Fig. 57B). Long basalia (L = 6 cm) arranged as a tube, protruding from the lower end of the habitus and anchored to the sediment (Fig. 57C).

Figure 57. 

Euplectellinae stet. CCZ_199 A in situ image B detail of body C whole specimen with protruding basalia. Scale bars: 1 cm (A); 5 mm (B). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B, C).

Remarks. The 16S sequence is close to Corbitella discasterosa Tabachnick & Lévi, 2004 (2.3% K2P distance), and it is also similar to other species within the family Euplectellidae. The closest COI match is Docosaccus maculatus Kahn, Geller, Reiswig & Smith Jr., 2013 (91.5% similarity). Morphological characters are concordant with those of the family Euplectellidae, and in the phylogenetic analysis it is recovered within the Euplectellidae (Fig. 54), along with other species of Holascus Schulze, 1886, but poorly supported. Although the subfamilies were not recovered as monophylectic, it is very likely it belongs to the subfamily Euplectillinae based on its lophophytous form.

Ecology. This specimen was found anchored to the abyssal sediments of APEI 1 at 5202 m depth.

Comparison with image-based catalogue. A very similar Euplectellidae morphotype (i.e., Euplectellidae gen. indet., HEX_005) has been commonly encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ, but not in abyssal areas of the Kiribati EEZ. Kersken et al. (2019) collected a few specimens of HEX_005 at the APEI 3 (Northeastern CCZ), identified as Corbitella discasterosa based on morphological characters. Corbitella discasterosa is basiphytous, while our specimen is lophophytous, but this would hardly be distinguished from seabed images.

Genus Docosaccus Topsent, 1910

Docosaccus sp. CCZ_021

Fig. 58

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 5.1043°N, 141.8867°W; 4860 m deep; 25 May. 2018; Smith & Durden leg.; GenBank: ON400684 (COI), ON406635 (18S), ON406612 (16S), ON406598 (28S), ON411255 (ALG11); NHMUK 2022.6; Voucher code: CCZ_021.

Description

Single specimen; lophophytous sponge. Plate-like, flat, subcircular body; 8.7 cm at its longest axis, 1 mm thick (Fig. 58A). Colouration is yellowish. Atrial surface facing up (Fig. 58C), and dermal surface almost in contact with the seafloor, with basalia protruding from it and anchoring it to the sediment (Fig. 58B).

Figure 58. 

Docosaccus sp. CCZ_021 A in situ image B dermal surface C and atrial surface. Scale bars: 1 cm (A, B, C). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B, C).

Remarks

External morphological characters are concordant with the description of D. maculatus (Kahn et al. 2013). However, sequences for the 16S and COI genes from the holotype are 2.4% and 3.9% divergent (K2P distance), respectively, from the western CCZ specimen. Sequences from 18S and 28S do not match to sequences from D. maculatus. The species was described from Station M, off California, in the Pacific Ocean at depths of 3,953–4,000 m, but the genus was originally thought to be restricted to Antarctica (Kahn et al. 2013). This species has been recorded in the CCZ, in the eastern IFREMER contract area and in APEI 3, from 4905–4998 m depth (Kersken et al. 2019). The specimen collected in the western CCZ differs from the holotype of D. maculatus in having more parietal oscula, and is smaller, and therefore considered a different species. External morphological characters also differ from the other species reported for the CCZ, D. nidulus Kersken, Janussen & Martínez Arbizu, 2019.

Ecology

This specimen was found anchored to abyssal sediments of APEI 7 at 4860 m depth.

Comparison with image-based catalogue

A very similar Docosaccus sp. morphotype (i.e., Docosaccus maculatus sp. inc., HEX_015) has been very frequently encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ and in abyssal areas of the Kiribati EEZ.

Genus Holascus Schulze, 1886

Holascus stet. CCZ_078

Fig. 59

Material

Clarion-Clipperton Zone • 1 specimen; APEI 7; 5.0443°N, 141.8162°W; 48745 m deep; 28 May. 2018; Smith & Durden leg.; GenBank: ON400700 (COI), ON406636 (18S), ON406613 (16S), ON406599 (28S), ON411256 (ALG11); NHMUK 2022.7; CCZ_078.

Description

Single specimen; lophophytous white sponge (Fig. 59A). Band-like body, collar formed from thin wall with two large openings, with lower opening being larger than upper opening (Fig. 59B, C). Body height of 4.8 cm, lower body diameter of 15.1 cm, and upper body diameter of 11.8 cm. basalia almost as long as the body height, protruding from the lower margin and anchoring it to the sediment (Fig. 59A).

Figure 59. 

Holascus stet. CCZ_078 A in situ image B lateral view of specimen before preservation C top view. Scale bars: 2 cm (A); 1 cm (B). Image attribution: Durden and Smith (A); Wiklund, Durden, Drennan, and McQuaid (B, C).

Remarks

Morphological external characters are concordant with the description of Holascus spinosus Kersken, Janussen & Martínez Arbizu, 2019, which was described from the IOM area in the CCZ. The closest genetic matches on GenBank for the 16S correspond to species within the genus Holascus (2.1–3.5% genetic divergence), with the holotype of Holascus spinosus being the closest match (2.1% genetic divergence). There are no 18S sequences available for H. spinosus, but it is 0.23% divergent from another species within the genus, H. euonyx (Lendenfeld, 1915), from which it differs morphologically. In the phylogenetic tree it was recovered, with low support, as sister to H. spinosus (Fig. 54), but 16S genetic divergence suggested these to be separate species.

Ecology

This specimen was found anchored to abyssal sediments of APEI 7 at 4874 m depth.

Comparison with image-based catalogue

A very similar Holascus sp. morphotype (i.e., Holascus sp. indet., HEX_014) has been commonly encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ and in abyssal areas of the Kiribati EEZ.

Subfamily Bolosominae Tabachnick, 2002

Bolosominae stet. CCZ_198

Fig. 60

Material. Clarion-Clipperton Zone • 1 specimen; APEI 1; 11.2518°N, 153.6053°W; 5205 m deep; 10 Jun. 2018; Smith & Durden leg.; GenBank: ON400728 (COI), ON406637 (18S), ON406614 (16S), ON406600 (28S); NHMUK 2022.10; Voucher code: CCZ_198.

Description. Single specimen; lophophytous white sponge (Fig. 60A, B). Body is cup- to bell-shaped, slightly wider than long (L = 10 cm, W = 11 cm), with a long, slender stalk (L = 70 cm) anchored to soft sediment. Central osculum with thick body walls.

Figure 60. 

Bolosominae stet. CCZ_198 A, C in situ images B specimen before preservation. Scale bars: 5 cm (A, C). Image attribution: Durden and Smith (A, C); Wiklund, Durden, Drennan, and McQuaid (B).

Remarks. The closest match with the 18S sequence is the holotype of Hyalostylus microfloricomus Kersken, Janussen & Martínez Arbizu, 2019 (99.8%), described from the Heip Mountains in the GSR contract area in the CCZ at 3788 m depth (Kersken et al. 2019). However, in the phylogenetic tree (Fig. 54) it was recovered in a well-supported clade representing the subfamily Bolosominae, but subclades were not well supported and hence the specimen is not attributed to any genera.

Ecology. This specimen was collected on abyssal sediments of APEI 1 at 5205 m depth, and was anchored to the sediment.

Comparison with image-based catalogue. A very similar stalked sponge morphotype (i.e., Hexactinellidae ord. indet., HEX_026) has been encountered in seabed image surveys conducted at the eastern CCZ, but not in abyssal areas of Kiribati’s EEZ. In seabed images, HEX_026 highly resembles Hyalonema (Cyliconemaoida) campanula Lendenfeld, 1915, as identified by Kersken et al. (2019) based on morphological traits observed in specimens encountered in the eastern CCZ.

Order Sceptrulophora Mehl, 1992

Family Euretidae Zittel, 1877

Subfamily Chonelasmatinae Schrammen, 1912

Genus Bathyxiphus Schulze, 1899

Bathyxiphus sp. CCZ_151

Figure 61

Material

Clarion-Clipperton Zone • 1 specimen; APEI 4; 6.9881°N, 149.9321°W; 5001 m deep; 06 Jun. 2018; Smith & Durden leg.; GenBank: ON400713 (COI), ON406638 (18S), ON406615 (16S), ON406601 (28S); NHMUK 2022.11; Voucher code: CCZ_151.

Description

Single specimen; basiphytous sponge (Fig. 61A, E). Body white, elongated (L > 60 cm, W = 10 cm), thin (W = 11 mm), upright-blade shaped habitus (Fig. 61B, C, E), attached to, possibly, a beaked-whale rostrum covered in manganese crust (Fig. 61A).

Figure 61. 

Bathyxiphus sp. CCZ_151. Specimen CCZ_151 A, E in situ images B, C, D specimen before preservation. Scale bars: 2 cm (B, C). Image attribution: Durden and Smith (A, E); Wiklund, Durden, Drennan, and McQuaid (B–D).

Remarks

Morphological characters are concordant with those of the genus, being very similar to Bathyxiphus subtilis Schulze, 1899, the only known species in the genus. However, a midrib has been suggested as a key morphological feature absent in the specimen presented here. The species was described from Isla Guadalupe at 1251 m depth, and was recently recorded in APEI 3 at 4914 m (Kersken et al. 2019). However, 16S sequences between the APEI 3 specimen and the western CCZ specimen are 3% divergent (K2P distance). Additionally, they were not recovered as monophyletic in the phylogenetic tree (Fig. 54) and hence considered different species. Measurements of total length were estimated from in situ images as only approx. half the specimen was recovered.

Ecology

The specimen was found attached to a beaked-whale rostrum covered in polymetallic crust, on abyssal sediments of APEI 4 at 5001 m depth.

Comparison with image-based catalogue

A similar Bathyxiphus sp. morphotype (i.e., Bathyxiphus sp. indet., HEX_025), though usually much smaller-sized, has been commonly encountered in seabed image surveys conducted across nodule fields areas of the eastern CCZ, but not in abyssal areas of the Kiribati EEZ.

Discussion

The DeepCCZ expedition surveyed three APEIs on the western CCZ, targeting both abyssal seafloor and seamounts, and sampling the different megafaunal components. The ROV survey for benthic megafauna yielded a remarkably diverse collection from a small number of specimens, with 48 species from only 55 specimens (Table 1). Most species were represented by a single specimen, but whenever more than one specimen of the same species was collected, they were found at similar depths and same geoform (i.e., seamount, abyssal plain or seamount slope), even if collected in different sites. More than half of the species, 26, were sediment dwellers found both on seamounts and abyssal plains, mostly representing mobile megafauna, such as sea urchins, sea cucumbers, brittle stars, sea stars, a polychaete worm, and a jellyfish observed skimming the seafloor, and a single sessile species of cup coral. Eight species, mainly sponges, were found anchored to the sediment, five species were found attached to nodules, two species attached to polymetallic crust found on seamount slopes, and a single species of sponge was found attached to a fossilised beaked-whale rostrum covered in manganese crust. Additionally, six species were found attached to old glass sponge stalks, including scalpellids, crinoids, and actiniarians, with a few species co-occurring on the same sponge stalks.

Many of the taxa presented here had been encountered in image-based surveys from across the CCZ but never collected before, making our collections particularly important for improving taxonomic knowledge (Glover et al 2018). We were able to obtain genetic sequences for all but one specimen. The vast majority of taxa (29) could not be attributed to described species, with only four having been previously reported for the CCZ (Christodoulou et al. 2020; Glover et al. 2016b; Pawson and Foell 1986). The high number of delimited but undescribed species reported here highlights our still-limited knowledge on abyssal invertebrate megafauna in the CCZ, especially in the protected APEI regions, and illustrates the importance of publishing open DNA taxonomic data, even if full species identification and description are not always possible.

Although the CCZ is often considered a vast and relatively homogeneous abyssal plain, this region has substantial, ecologically important, seafloor heterogeneity (McQuaid et al. 2020; Washburn et al. 2021a). Variations in community structure and biodiversity have been documented across the region (Smith et al. 2021), mainly in the central and eastern CCZ, resulting from local and regional environmental conditions, such as topographical changes, food input, and nodule abundance (Stoyanova 2012; Vanreusel et al. 2016; Simon-Lledó et al. 2019c). While this study was not designed to investigate drivers of benthic megafaunal assemblages, some differences were observed between seamounts and abyssal plains. In other areas, seamounts have been found to harbour similar megafaunal taxonomic compositions as adjacent continental slopes (Clark et al. 2010). However, little overlap between seamount and abyssal plain taxa has been reported from surveys in the eastern CCZ (Cuvelier et al. 2020), and in western APEIs (Bribiesca-Contreras et al. 2021; Durden et al. 2021; Leitner et al. 2021). Due to the sampling strategy of maximising diversity, most species were represented by a single specimen, but whenever more than one specimen from the same species was collected, they were found in the same geoform (i.e., seamount or abyssal seafloor) even when collected in different APEIs (Table 1). Eight of the nine species that were assigned to previously described taxa were found in abyssal sites, except for Calyptrophora distolos that was originally described from a seamount (Cairns 2018). This is possibly a result of seamounts being understudied and undersampled compared to abyssal plains.

While the lack of shared species between habitats in this study could be a result of undersampling, faunistic changes between habitats have been reported in the CCZ (Cuvelier et al. 2020; Durden et al. 2021). Differences between abyssal plains and seamounts in APEIs 4 and 7 (with only 16 and 19% overlap in Operational Taxonomic Units; OTUs) have been observed from environmental-DNA sampling of sediments, with most OTUs being rare and limited to small areas (Laroche et al. 2020). Similarly, image-based surveys in the western APEIs observed very few common morphotypes shared across habitats, with several rare morphotypes observed only once (Durden et al. 2021). In addition, Leitner et al. (2021) have argued that even if some species are shared between seamounts and the abyssal seafloor, the areal coverage of seamounts is a tiny proportion compared to the areal of abyssal seafloor in the CCZ, so seamounts could supply only a very limited number of propagules for recolonisation over ecological time scales. Previous findings and our data currently suggest that CCZ seamounts could not act as viable refugia for abyssal taxa and provide propagules for recolonisation of areas disturbed by mining. Following the principles of a precautionary approach, representative and suitable refuge areas for megafaunal communities needs to be defined and protected as source of propagules for recolonisation.

The study of deep-sea ecosystems presents several challenges, from the difficulties of collecting at great depths, to the resources required for an oceanographic survey, and the labour to document and describe all the collected material (Glover et al. 2018). With the pressing need to describe the biodiversity of these habitats, taxonomic studies that synthesise morphological, ecological, and genetic information, even for undescribed and indeterminate species (e.g., Dahlgren et al. 2016; Glover et al. 2016b; Amon et al. 2017a, b; Wiklund et al. 2017; Christodoulou et al. 2020) contribute fundamentally to the iterative building of biodiversity baselines (Engel et al. 2021; Glover et al. 2015). For instance, based on DNA sequences, we found two taxa with distributions likely spanning the entire CCZ, because they matched previously indeterminate juveniles collected in the eastern CCZ (Ophiocymbium tanyae as Ophioscolecidae sp. 20 in Christodoulou et al. (2020), and cf. sp. CCZ_165 as Crinoidea sp. NHM_055 in Glover et al. (2016b)).

One of the limitations of this study is the scarcity of published barcodes for deep-see invertebrates. For instance, an environmental DNA study in the western APEIs found that only 25% and 1.5% of OTUs could be assigned to family level using reference libraries for 18S and COI, respectively (Laroche et al. 2020). We mainly targeted the barcoding gene COI because it has been used before to document biodiversity in the CCZ (e.g., Dahlgren et al. 2016; Glover et al. 2016b; Wiklund et al. 2017; Christodoulou et al. 2020). However, while the gene is useful for species delimitation in most groups, it is so variable that it does not accurately reflect phylogenetic relationships. Thus, in several cases it was not possible to assign our specimens to lower taxonomic levels based solely on genetic information because comparisons of our generated sequences to public databases showed that the closest taxa were only ~ 80% similar to multiple species belonging to different higher taxonomic ranks (Order or Family levels). Hence, additional genes were targeted to resolve higher rank relationships (16S, 18S, and 28S), but even for these genes, the lack of reference libraries hindered taxonomic placement. This is not surprising because the deep sea is severely undersampled and genetic sequences from bathyal and abyssal species are scarce in public databases. Even though there is much greater availability of genetic information for shallow water species, this did not inform our taxonomic assignments for most groups, because it has been found that bathyal and abyssal taxa often represent separate lineages from shallow-water ones (e.g., brittle stars: Bribiesca-Contreras et al. 2017; Christodoulou et al. 2019; isopods: Lins et al. 2012).

Additional challenges result from the documentation that COI and other mitochondrial genes show very little variation in anthozoan cnidarians (Hebert et al. 2003), preventing use of COI for species delimitation. For instance, the sequence of Chrysogorgia sp. CCZ_112 collected in APEI 4 differed from the COI sequence of C. abludo by a single nucleotide. Although the specimen was not assigned to the species because previous findings show that congeneric species of octocorals can share the same COI haplotype (McFadden et al. 2011), we also took a precautionary approach when assigning species to avoid overestimation of species ranges. In some syntheses, most deep-sea species have been assumed to have wide distributional ranges (McClain and Hardy 2010), likely resulting from the greater ease of discovering abundant, wide-ranging species than species with narrow distributions (Higgs and Attrill 2015), but also due to low or overlooked morphological variability that hinders our ability to discriminate species. Molecular studies have revealed different distinct lineages in some common, previously considered wide-ranging species. For instance, genetic sequences and further detailed morphological examination revealed at least two distinct species within the cosmopolitan Psychropotes longicauda Théel, 1882 (Gubili et al. 2017; Gebruk et al. 2020). One represents the cosmopolitan species P. pawsoni Gebruk & Kremenetskaia in Gebruk et al. 2020 distributed in all four oceans, and the second represents a species only occurring in the northwest Pacific, P. moskalevi Gebruk & Kremenetskaia in Gebruk et al. 2020.

While detailed taxonomic studies can reveal an overlooked biodiversity in deep-sea taxa and are greatly improving our understanding of species ranges, they are time-consuming and usually target a small area or a few taxa (e.g., O’Loughlin and Ahearn 2005; Molodtsova and Opresko 2017; Herzog et al. 2018; Kersken et al. 2019). In contrast, the use of in situ seabed imagery to investigate megabenthic communities allows much larger area surveys, but at a lower taxonomic resolution that can potentially underestimate biodiversity and over-estimate species ranges. For instance, the sponge subfamilies Euplectellinae and Corbitellinae are differentiated by their mode of fixation to substrata. From seabed imagery, two hexactinellid sponges belonging to these subfamilies (Euplectellinae stet. CCZ_199 herein, and Corbitella discasterosa in (Kersken et al. 2019), respectively) were classified as the same morphotype because the mode of fixation cannot be observed when photographed from the top. However, the integration of both methods can provide key insights into species composition and connectivity of benthic communities across the CCZ, which are fundamental aspects to guide conservation strategies.

The alignment of in situ specimen images from this study with invertebrate morphotypes previously catalogued from (and standardised across) different seabed image surveys conducted in the CCZ (Amon et al. 2016; Simon-Lledó et al. 2019c, d, 2020; Cuvelier et al. 2020; Durden et al. 2021) provided preliminary insight into the connectivity of western megafauna populations. The 53 specimens that had an associated in situ image were classified into a total of 45 morphotypes, from which 11 represented new additions to the existing CCZ megafauna catalogue. Few morphotypes (9) were found to have wider distributions, being reported from both the Kiribati EEZ to the west of the CCZ, and from the eastern CCZ. Surprisingly, only two morphotypes were uniquely shared with Kiribati, while 16 were uniquely shared with the eastern CCZ. These results suggest, rather tentatively, that western CCZ megafauna communities may share a much larger species pool with (the more distant) eastern CCZ areas than with closer areas towards the west, like Kiribati’s abyss. However, it is important to note that a much larger sampling effort was previously conducted in eastern CCZ areas than around Kiribati. In addition, the ROV sampling conducted for this study was limited and the number of species identified in this study (48) is much lower than the number of morphotypes that have been identified from seabed imagery in the western CCZ (143; Durden et al. 2021). Further interpretation and synthesis of existing megafauna distribution data, as well as additional sampling, will expand and contextualise these preliminary observations.

Conclusions

We provide the first megafaunal faunistic study from the western CCZ based on voucher specimens. Our findings indicate a high diversity, represented mostly by undescribed species of megafauna in the western CCZ with little overlap between abyssal plains and seamounts, and within similar habitats located in greater distances to one another. Further studies should aim to increase our knowledge of patterns of biodiversity across the entire CCZ in order to inform environmental management plans to protect its biodiversity. Our work also highlights the need for detailed taxonomic studies, not only within the CCZ, an area targeted for deep-sea mining, but in other bathyal, abyssal, and hadal regions. While species identification through genetic markers can facilitate the generation of species inventories, this is only achievable when genetic reference libraries are representative of the area and taxon of study, and these remains limited for the CCZ megafauna.

Acknowledgements

We want to acknowledge the masters, crew and technical support staff on the R/V Kilo Moana and ROV Lu’ukai during the DeepCCZ expedition. We are very grateful for help with taxonomic identifications provided by the expert taxonomists: Estefania Rodriguez (AMNH) for anemones, Dhugal Lindsay (JAMSTEC) for scyphozoans; Chris Mah (NMNH) for sea stars; Rich Mooi (CAS) and Carlos Andres Conejeros Vargas (UNAM) for sea urchins; and Lenka Nealova (NHM) for annelids. We also acknowledge Lauren Hughes (NHM), Miranda Lowe (NHM), Tom White (NHM), Amanda Robinson (NMNH), Emma Sherlock (NHM), Andreia Salvador (NHM), and Andrew Cabrinovic (NHM) for curatorial support; and Elena Luigli (NHM) and Claire Griffin (NHM) for lab support. Primary funding was from the Gordon and Betty Moore Foundation grant no. 5596 and NOAA Office of Ocean Exploration (grant #NA17OAR0110209), and the University of Hawaii. We also acknowledge funding from UK Seabed Resources, the UK Natural Environment Research Council grant numbers NE/T003537/1 and NE/T002913/1 and through National Capability funding to NOC as part of the Climate Linked Atlantic Section Science (CLASS) programme (grant number NE/R015953/1), and The Norwegian Research Council (JPIOMining Impact 2). DJA received funding from the EU’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement number 747946.

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Supplementary material

Supplementary material 1 

Table S1

Guadalupe Bribiesca-Contreras, Thomas G. Dahlgren, Diva J. Amon, Stephen Cairns, Regan Drennan, Jennifer M. Durden, Marc P. Eléaume, Andrew Hosie, Antonina Kremenetskaia, Kirsty McQuaid, Timothy D. O’Hara, Muriel Rabone, Erik Simon-Lledó, Craig R. Smith, Les Watling, Helena Wiklund, Adrian G. Glover

Data type: phylogenetic

Explanation note: Detail of sequences included in the phylogenetic analyses including details of voucher numbers, code, GenBank accession number for different genes, details on whether specimens included are part of type material.

This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.
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