Research Article |
Corresponding author: Daode Yang ( csfuyydd@126.com ) Academic editor: Annemarie Ohler
© 2023 Tianyu Qian, Yonghui Li, Jun Chen, Pipeng Li, Daode Yang.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Qian T, Li Y, Chen J, Li P, Yang D (2023) Tadpoles of four sympatric megophryinid frogs (Anura, Megophryidae, Megophryinae) from Mangshan in southern China. ZooKeys 1139: 1-32. https://doi.org/10.3897/zookeys.1139.81641
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Sympatric distribution and potentially long larval development time make the assignment of tadpoles confusing in Asian-horned frogs of the subfamily Megophryinae. In this study, we used molecular data to identify four syntopic megophryinid tadpoles from Mangshan on the border between Hunan and Guangdong provinces in southern China: Brachytarsophrys popei, Boulenophrys shimentaina, Bo. cf. ombrophila, and Bo. nanlingensis. A detailed re-description of the Br. popei tadpoles is provided as well as the first descriptions of three Boulenophrys tadpoles based on external morphology and coloration. An effort is attempted to distinguish these tadpoles by coloration patterns: the dorsal pattern, ventral pattern, and pattern on tail are useful for field identification of these tadpoles. However, the variation of color pattern could sometimes make species delineation difficult. Researchers are encouraged to document coloration in life with photographs and the collection of tadpoles of different development stages and sizes advocated in order to better understand how color may change during larval development.
Amphibian, integrative taxonomy, larvae, Megophrys, Nanling Mountains
The Asian Horned Frogs in the subfamily Megophryinae Bonaparte, 1850 belong to seven genera and 129 recognized species with wide distributed in Asia, ranging from India and Bhutan to China and south to the Sundas and the Philippines (
Seven monophyletic clades were found within Megophryinae based on molecular analysis (
Before molecular analysis was widely used in megophryinid taxonomic studies, tadpoles were assigned to species based on their association with post-metamorphic/adult specimens collected from the same site, e.g., Boulenophrys jingdongensis (Wang in
Mangshan, a part of Nanling Mountains, is located on the border between Hunan and Guangdong provinces in southern China. The first megophryinid record from this area was a tadpole with a funnel-like oral disc collected at the mountain top of Guangdong/Hunan border (
In this study, we collected tadpoles bearing funnel-like oral disc from Mangshan. Using DNA barcoding, we confirmed that this collection of syntopic tadpoles was composed of four species: Brachytarsophrys popei, Boulenophrys nanlingensis (Lyu, Wang, Liu & Wang in
All tadpoles were collected from Mangshan, Yizhang, Hunan Province, China during field surveys in June, July, and November 2021. Specimens were photographed in life in a transparent acrylic box before being euthanized with buffered tricaine methanesulfonate (MS-222) and then fixed with 10% formalin for storage. Tissue samples (tail fin/muscle) were preserved in 95% ethanol for molecular analysis. Specimens were deposited at the Institute of Wildlife Conservation, Central South University of Forestry and Technology (CSUFT), Changsha, China.
Segments of the 16S ribosomal RNA gene (16S) were used for species identification. Primer sequences (L3975 and H4551) from
Image J 1.53k software (
BH body height, maximal body height at trunk;
BL body length from snout to the point where the axis of the tail myotomes meets the body wall;
BS body end to the center of spiracle;
BW maximal body width;
ED eye diameter;
ES eye–snout distance;
IND the internarial distance measured from center to center;
IOD the interorbital distance measured from center to center;
MTH maximal tail height;
LFH lower fin height at MTH;
UFH upper fin height at MTH;
NE distance from the center of naris to the center of the eye;
ODW oral disc width;
SN distance from the center of naris to snout;
SS distance from snout to the center of spiracle;
TTL total length;
TAL tail length = TTL – BL;
TMH tail muscle height at the body-tail junction, where ventral line of musculature meets trunk contour;
TMW tail muscle width at the same level as TMH.
For the measurement of oral disc width (ODW) in preserved specimens, we expanded the oral disc by anchoring it to a glass (as shown in Fig.
We compared the tadpoles with their congeneric tadpoles described in the literature where molecular data has been used to confirm species identity: Br. popei, tadpoles described by
Two tadpoles were identified as Brachytarsophrys popei based on an uncorrected p–distance of 0.0–0.7% from the samples in the type series from Hunan, Guangdong, and Jiangxi Provinces (GenBank accession numbers: KM504251–KM504258). Three tadpoles from the same collection site and bearing the same characteristics as the above two tadpoles but without molecular data were also assigned to Br. popei. The collection site of these tadpoles is only ~ 5 km from the collection site of paratype SYS a00589 (GenBank accession number: KM504051) in the adjacent Nanling Nature Reserve, Guangdong Province.
A total of 14 tadpoles was identified as Boulenophrys nanlingensis, which exhibited an uncorrected p–distance of 0.0–0.4% from the holotype SYS a001964 (GenBank accession number: MH406646) collected ~ 10 km in the adjacent Nanling Nature Reserve, Guangdong Province.
Five tadpoles were identified as Boulenophrys shimentaina, which exhibited an uncorrected p–distance of 1.2% from the type series (GenBank accession numbers: MH406655–MH406656, and 787–788) collected from Shimentai Nature Reserve, Guangdong Province, China ~ 70 km to the south of Mangshan. This genetic distance is smaller than the interspecies p–distance in the subfamily Megophryinae used to identify tadpoles (1.4% in Br. intermedia,
Four tadpoles showed an uncorrected p–distance of 1.8–2.1% from the type series of Bo. ombrophila (GenBank accession numbers: KX856397–KX856404) collected from Wuyishan, Fujian Province, China ~ 500 km to the northeast of Mangshan. This genetic distance almost equals to the threshold (2.0% in 16S gene, proposed by
All examined specimens exhibited a funnel-like oral disc corresponding to the tadpole description of “Megophrys minor” by
The following description is based on five tadpoles at Stages 26–27 (N = 2) and 36–37 (N = 3). Body ratio ranges represent all specimens. Raw measurements are given in Table
Morphometric data of the tadpole specimens used in this study. For abbreviations, see Materials and Methods. “*” indicates specimens with broken tails, and “\” indicates “no data”.
Species | Voucher No. | Stage | TTL | BL | TAL | BH | SS | BS | ED | TMH | MTH | UFH | LFH | IOD | IND | NE | SN | ES | TMW | BW | ODW |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Brachytarsophrys popei | CSUFT T10944 | 27 | 31.8 | 9.0 | 22.8 | 4.2 | 5.0 | 4.2 | 1.2 | 3.0 | 5.4 | 1.7 | 1.9 | 3.8 | 2.5 | 1.1 | 1.6 | 2.7 | 2.7 | 4.8 | 5.4 |
CSUFT T10945 | 26 | 27.9 | 7.9 | 20.0 | 3.9 | 4.9 | 3.4 | 1.2 | 2.9 | 5.2 | 1.4 | 1.6 | 3.8 | 2.6 | 1.1 | 1.5 | 2.6 | 2.5 | 4.4 | 5.9 | |
CSUFT T10115 | 37 | 35.0 | 10.5 | 24.5 | 4.7 | 6.1 | 4.5 | 1.4 | 3.1 | 6.4 | 2.1 | 2.0 | 4.6 | 2.8 | 1.4 | 1.9 | 3.3 | 2.9 | 5.8 | 7.4 | |
CSUFT T10117 | 37 | 37.3 | 11.0 | 26.3 | 5.0 | 6.3 | 4.7 | 1.5 | 2.9 | 5.8 | 1.9 | 1.9 | 4.7 | 2.8 | 1.5 | 2.0 | 3.4 | 2.9 | 5.9 | 7.8 | |
CSUFT T10119 | 36 | 36.9 | 10.9 | 26.0 | 5.0 | 6.3 | 4.9 | 1.4 | 3.2 | 6.3 | 2.1 | 2.1 | 4.7 | 3.0 | 1.5 | 1.8 | 3.2 | 3.1 | 5.9 | 7.6 | |
Boulenophrys shimentaina | CSUFT T10156 | 25 | 28.5 | 7.3 | 21.2 | 3.1 | 3.9 | 3.8 | 0.8 | 2.3 | 4.4 | 1.2 | 1.1 | 3.1 | 2.2 | 1.0 | 1.4 | 2.3 | 2.4 | 4.0 | 5.2 |
CSUFT T10277 | 26 | 28.6 | 8.0 | 20.6 | 3.8 | 4.6 | 3.5 | 1.0 | 2.5 | 4.5 | 1.3 | 1.3 | 3.4 | 2.4 | 1.0 | 1.6 | 2.6 | 2.4 | 4.4 | 6.3 | |
CSUFT T10279* | 25 | \ | 8.3 | \ | 3.7 | 4.7 | 3.9 | 1.0 | 2.6 | \ | \ | \ | 3.4 | 2.3 | 1.0 | 1.5 | 2.4 | 2.5 | 4.3 | 6.6 | |
CSUFT T10285 | 27 | 28.5 | 8.1 | 20.4 | 3.7 | 4.7 | 3.6 | 1.0 | 2.8 | 5.6 | 1.5 | 1.5 | 3.4 | 2.3 | 1.0 | 1.5 | 2.4 | 2.4 | 4.4 | 5.7 | |
CSUFT T10283 | 28 | 27.0 | 8.0 | 19.0 | 3.5 | 4.4 | 3.7 | 0.9 | 2.6 | 4.8 | 1.3 | 1.2 | 3.2 | 2.3 | 0.9 | 1.4 | 2.3 | 2.2 | 4.1 | 6.0 | |
Boulenophrys nanlingensis | CSUFT T10144 | 25 | 18.7 | 5.4 | 13.3 | 2.3 | 3.2 | 2.5 | 0.7 | 1.7 | 2.7 | 0.7 | 0.8 | 2.3 | 1.6 | 0.7 | 1.0 | 1.7 | 1.5 | 2.8 | 3.8 |
CSUFT T10986 | 35 | 40.1 | 10.8 | 29.3 | 5.4 | 6.4 | 4.5 | 1.6 | 3.7 | 7.3 | 1.7 | 1.6 | 4.7 | 3.2 | 1.5 | 2.4 | 3.7 | 3.6 | 5.7 | 7.8 | |
CSUFT T10969 | 34 | 34.4 | 8.8 | 25.6 | 4.0 | 5.6 | 4.2 | 1.3 | 3.0 | 5.5 | 1.4 | 1.3 | 3.9 | 2.7 | 1.1 | 1.9 | 3.0 | 2.8 | 4.8 | 5.6 | |
CSUFT T10261 | 25 | 25.1 | 6.7 | 18.4 | 3.1 | 4.2 | 2.7 | 0.8 | 1.8 | 4.0 | 1.2 | 1.2 | 2.7 | 1.9 | 0.8 | 1.2 | 2.0 | 1.5 | 3.8 | 5.4 | |
CSUFT T10262 | 25 | 27.2 | 6.5 | 20.7 | 2.8 | 4.0 | 2.8 | 1.0 | 1.9 | 4.2 | 1.2 | 1.2 | 2.9 | 1.9 | 0.9 | 1.2 | 2.1 | 1.7 | 3.6 | 5.2 | |
CSUFT T10273 | 28 | 35.7 | 9.4 | 26.3 | 4.4 | 5.4 | 4.3 | 1.1 | 3.2 | 5.9 | 1.5 | 1.5 | 4.0 | 2.8 | 1.0 | 1.8 | 2.9 | 3.0 | 5.0 | 7.9 | |
CSUFT T10991 | 27 | 39.1 | 10.3 | 28.8 | 4.4 | 6.0 | 4.4 | 1.3 | 3.1 | 6.7 | 2.0 | 1.8 | 4.0 | 2.8 | 1.2 | 1.9 | 3.1 | 2.8 | 5.3 | 7.6 | |
CSUFT T10284 | 25 | 18.9 | 5.3 | 13.6 | 2.6 | 3.3 | 2.2 | 0.8 | 1.7 | 3.4 | 1.0 | 0.9 | 2.6 | 1.6 | 0.8 | 1.0 | 1.8 | 1.6 | 3.2 | 4.1 | |
CSUFT T10302* | 25 | \ | 7.3 | \ | 3.3 | 4.0 | 3.3 | 0.9 | 2.4 | \ | \ | \ | 3.0 | 2.0 | 0.9 | 1.3 | 2.2 | 2.1 | 4.0. | 6.0 | |
CSUFT T10303 | 25 | 26.2 | 6.8 | 19.4 | 3.3 | 3.9 | 3.1 | 0.8 | 2.3 | 4.0 | 1.3 | 1.2 | 2.9 | 1.9 | 0.9 | 1.3 | 2.2 | 2.0 | 3.9 | 4.9 | |
CSUFT T10377 | 27 | 28.1 | 8.2 | 19.9 | 3.5 | 4.9 | 3.4 | 1.0 | 2.3 | 5.3 | 1.5 | 1.5 | 3.4 | 2.3 | 0.9 | 1.6 | 2.4 | 2.0 | 4.4 | 6.8 | |
CSUFT T10378 | 28 | 26.9 | 8.2 | 18.7 | 3.4 | 5.0 | 3.2 | 1.2 | 2.4 | 5.2 | 1.3 | 1.4 | 3.4 | 2.2 | 0.9 | 1.4 | 2.3 | 2.2 | 4.2 | 5.9 | |
CSUFT T10376 | 27 | 24.8 | 7.0 | 17.8 | 3.5 | 4.1 | 3.1 | 1.0 | 2.1 | 4.8 | 1.4 | 1.4 | 3.1 | 2.1 | 0.9 | 1.3 | 2.1 | 2.0 | 4.0 | 5.7 | |
CSUFT T10379 | 29 | 27.8 | 7.7 | 20.1 | 3.5 | 4.5 | 3.1 | 1.2 | 2.5 | 5.0 | 1.3 | 1.4 | 3.1 | 2.2 | 1.0 | 1.4 | 2.3 | 1.9 | 4.0 | 5.7 | |
Boulenophrys cf. ombrophila | CSUFT T10270 | 36 | 33.7 | 10.0 | 23.7 | 4.5 | 5.6 | 4.6 | 1.4 | 2.9 | 6.0 | 1.4 | 1.6 | 4.2 | 2.8 | 1.4 | 1.7 | 3.0 | 2.9 | 5.1 | 8.3 |
CSUFT T10272 | 27 | 33.1 | 8.9 | 24.2 | 4.1 | 5.3 | 4.2 | 1.2 | 3.0 | 6.0 | 1.4 | 1.4 | 3.8 | 2.5 | 1.1 | 1.7 | 2.8 | 2.8 | 4.8 | 7.8 | |
CSUFT T10288 | 26 | 30.4 | 8.4 | 22.0 | 3.9 | 4.8 | 3.8 | 1.1 | 2.7 | 5.9 | 1.6 | 1.4 | 3.6 | 2.4 | 1.1 | 1.5 | 2.6 | 2.6 | 4.6 | 7.2 | |
CSUFT T10992 | 25 | 20.9 | 5.1 | 15.8 | 2.1 | 2.8 | 2.0 | 0.5 | 1.6 | 3.0 | 0.8 | 0.8 | 2.2 | 1.5 | 0.6 | 1.0 | 1.7 | 1.3 | 2.7 | 3.9 |
CSUFT T10115 (Stage 37, Field voucher: MT05; GenBank accession number: ON209276), CSUFT T10117 (Stage 37; Field voucher: MT07; GenBank accession number: ON209284), and CSUFT T10119 (Stage 36; Field voucher: MT09; not sequenced), collected on 30 May 2021 from Tiantaishan (24.972277°N, 112.963394°E, ca. 1280 m a.s.l.), Mangshan, Hunan Province, China; and CSUFT T10944 (Stage 27, Field voucher: MT1104; not sequenced), and CSUFT T10945 (Stage 26; Field voucher: MT1105; not sequenced), collected on 16 November 2021 from the same site as the first specimens.
The body is oval, robust, and flattened above (BW/BL 53.3–55.7% at Stages 26–27, N = 2; and 53.6–55.2% at Stages 36–37, N = 3); the head is wider than the trunk; the eyes are located dorsolaterally, the pupils are round; the nares are oval, opening laterally, closer to the eye than to the tip of the snout (NE/SN 68.8–73.3% at Stages 26–27, N = 2; and 73.7–83.3% at Stages 36–37, N = 3); the internarial distance is smaller than the interorbital distance (IND/IOD 65.8–68.4% at Stages 26–27, N = 2; and 59.6–63.8% at Stages 36–37, N = 3); the rims of nares are raised from the body wall and directed posterolaterally; the spiracle is sinistral and low on the left flank; the spiracle tube is short, protruding posterodorsally, free from the body at the tip, and opening posterolaterally (SS/BL 55.6–62.0% at Stages 26–27, N = 2; and 57.3–58.1% at Stages 36–37, N = 3); the anal tube opens medially, unattached to the ventral fin; the dorsal fin arises behind the body-tail junction while the ventral fin is connected to the trunk; the tail muscle is massive, taller than tail fins before reaching the maximum tail height (TMH/MTH 55.6–55.8% at Stages 26–27, N = 2; and 48.4–50.8% at Stages 36–37, N = 3), and the tail tip is bluntly pointed, the tail length accounts for 71.7% (at Stages 26–27, N = 2) and 70.5–70.5% (at Stages 36–37, N = 3) of the total length; the mouth is terminal and the oral disc is funnel-like (BW/ODW 74.6–88.9% at Stages 26–27, N = 2; and 75.6–78.4% at Stages 36–37, N = 3); three and four rows of short oval submarginal papillae can be observed on the upper lip and lower lip, respectively; keratodonts are absent; the upper jaw sheath is brush-like, exhibiting a small median notch, while the lower jaw sheath is thin, sickle-shaped, weakly keratinized, and finely serrated.
In life, the background color of the head and trunk is dark brown; the dorsal pattern is pale brown interspersed with dark brown chromocytes, extending to above the horizontal level of the spiracle on the trunk from a lateral perspective; the dorsal surface of the anterior part of the tail is pale brown marbled with dark brown speckles; neuromasts are distinctly visible on the head, trunk and tail; the region between the anterior edges of the eyes and the median point of the upper lip is pigmented with a dark brown V-shaped pattern; the narial rims are pale brown; the oral disc is golden-pigmented, with a translucent edge; the submarginal papillae on lips are dark brown-pigmented. Laterally, the tail is pale brown-pigmented; dense goldish spots are located at the anterior part of the lateral surface of tail muscle, becoming smaller and at the middle, then disappearing posteriorly; three distinct dark brown stripes extended from the body-tail junction, and horizontally positioned at the anterior part of the tail; the upper and lower stripes end before reaching the maximum tail height, while the middle stripe is about half the length of the others; the upper and middle stripes are incomplete; the anterior part of the upper fin is opaque, marbled with goldish pigmentation and brown speckles; the anterior part of the ventral fin, as well as the anal tube are semi-translucent with dense large golden spots; the rest of the fins are semi-translucent, and exhibit sparse dark brown speckles interspersed with small goldish dots. The ventral surface of the body is rather dark; the belly is dark purplish covered with dense white spots; two longitudinal stripes, positioned ventrolaterally, extending from the snout to the vertical edge of the eyes posteriorly, and sometimes appear to broken; a transverse bar is positioned at the head-trunk junction of the vertical edge of the anterior spiracle and is always interrupted at the middle; the spiracle region and the corresponding region on the other side of the body, are covered with a short white stripe, that starts from the head-body connection, and terminated before reaching the region of the spiracle tube opening; regions without white pigmentation have less melanocytes; the gills and gut coils are indistinctly visible through the ventral skin. The eye sclera is silver with black dots; the iris periphery is wide and black; the iris is golden sprinkled with black dots; and the spiracle is translucent without pigmentation. In tadpoles at Stages 36–37, the hindlimbs are semi-transparent, and the outer aspect of the legs exhibits brown pigmentation interspersed with goldish chromocytes.
In preserved specimens, the tail stripes are still prominent; an incomplete V-shaped pigmentation pattern is still visible; the ventral pattern is translucent milky white; the golden pigmentation wanes on the oral disc; and the hindlimb bones are visible in ventral view in Stage 36–37 tadpoles.
Brachytarsophrys popei tadpoles in life A–C tadpole CSUFT T10117 (Stage 37) lateral view, dorsal view, and ventral view D ventral pattern of tadpole CSUFT T10119 (Stage 36) E ventral pattern of tadpole CSUFT T10945 (Stage 26); and F oral disc of tadpole CSUFT T10117 (Stage 37). D and E share the same scale bar with A–C.
Tadpoles of Br. popei differ significantly from the three syntopic Boulenophrys tadpoles described below by the unique pattern of two longitudinal white ventrolateal stripes on head, a transverse white bar on chest, and distinct large spots on belly (vs. absence of stripes and bars, and smaller spots/speckles on belly).
The differences in ventral pattern between four Brachytarsophrys tadpoles were compared by
Comparison of color pattern among tadpoles of the subfamily Megophryinae which were identified based on molecular data. “*” indicates characteristics not mentioned in the text but were illustrated in the figure, and “\” indicates “no data”.
Species | Stage | Neuromasts visibility | Intestine visibility | Dorsum pattern | Pattern on tail | Ventral pattern | References |
---|---|---|---|---|---|---|---|
Atympanophrys | |||||||
A. gigantica | 35, N = 5 | visible | visible | uniform dark brown | pale yellowish brown without speckles | translucent dark grey and speckled with white |
|
Brachytarsophrys | |||||||
Br. popei | 26–27, and 36–37, N = 5 | distinct | indistinct | uniform dark brown | small dots and longtitudinal stripes | ventrolateral stripes on head and body, incomplete transverse bar on chest, dense large spots on belly | This study |
26–29, N = 14 | \ | \ | \ | three dark longitudinal stripes | two longitudinal white stripes along the sides of body, a completed transverse bar on chest, belly mottled with dense white speckles |
|
|
Br. intermedia | 32, 36, and 39, N = 4 | pale brown | not visible | pale brown with a darker brown medial saddle | speckled with dark brown, and longitudinal stripes | ventrolateral stripes on head and body, small spots on chest and belly |
|
Br. chuannanensis | 38, N = 1 | \ | \ | \ | distinct dark longtitudinal stripes* | wide ventrolateral stripes on head*; wide transverse bar on chest; and several spots on belly* |
|
Br. orientalis | 36, N = 1 | \ | \ | brown | three short dark longitudinal stripes | two short, longitudinal white stripes on sides of ventral surface of head and body; absence of transversal white stripe on chest; belly mottled with dense white speckles |
|
Br. feae | 44, N = 1 | \ | \ | \ | \ | transeverse bar on chest; several several transeverse stripes on belly |
|
Boulenophrys | |||||||
Bo. shimentaina | 25–28, N = 5 | distinct | visible | brown with dark brown reticulation | pigmented with dense dark brown markings posteriorly | milky white ventrolateral spots on chest, dense indistinct small milky white speckles on belly | This study |
Bo. cf. ombrophila | 25, N = 1 (TTL 20.9 mm) | indistinct | distinct | pale brown, scattered with dense dark melanocytes | pigmented orange and dark brown speckles | belly covered with dense melanocytes | This study |
26–27, and 36, N = 3 (TTL 30.4–33.1 mm) | distinct | indistinct | brown pattern along mid-vertical line | several large brown spots along tail muscle | gold-pigmented white ventrolateral spots on chest, dense white speckles on belly | This study | |
Bo. nanlingensis | 25, N = 2 (TTL 18.7–18.9 mm) | distinct | distinct | yellowish with pale orangish blotches, or brown with whitish patterns | many brown speckles | gold-pigmented white ventrolateral spots on chest, sparse white speckles on belly | This study |
25, N = 3 (TTL 25.1–27.2 mm) | distinct | distinct | pale brown with dark brown pigmentation | many brown speckles | gold-pigmented white ventrolateral spots on chest, sparse white speckles on belly | This study | |
27–29, N = 4, TTL 24.8–28.1 mm) | distinct | distinct | bi-colored dorsum of pale brown anteriorly and dark brown posteriorly | many brown speckles | gold-pigmented white ventrolateral spots on chest, sparse white speckles on belly | This study | |
27–28, and 34–35, N = 4, (TTL 35.7–44.4 mm) | distinct | distinct | uniform brownish | many brown speckles | gold-pigmented white ventrolateral spots on chest, sparse white speckles or dense large spots on belly | This study | |
Bo. fansipanensis | 25, N = 2 | obvious | visible | brown with dark brown speckles | small spots and dark brown speckles | a translucent grey brown and speckled with metallic blue and flecked with dark brown | This study |
Bo. jingdongensis | 25, N = 1 | indistinct | visible | dark brown with cream blotches, bordered by orange flecks | many dark brown speckles | grey brown and speckled with metallic blue |
|
Bo. hoanglienensis | 26, N = 1 | distinct | visible | dark brown with reddish brown blotches and reticulated blackish brown | many dark brown speckles | speckled with metallic grey blue flecks |
|
Bo. rubrimera | 37, N = 1 | obvious | \ | brown with darker speckles | pale yellowish brown with speckles | speckled white and brown |
|
Bo. baishanzuensis | 31, N = 1 | \ | \ | brownish black | small white and black spots | \ |
|
Bo. lushuiensis | 26–27, 32, and 36, N = 5 | \ | visible | brown without distinct patterns | pale brown with dozens of small dark brown patches | scattered with silver tiny patches |
|
Bo. leishanensis | 25–26, N = 6 | visible* | \ | yellow-brown | pale colored on fins, and small black spots on tail muscle | dense small white speckles* |
|
Bo. jiangi | 26, N = 2 | \ | \ | yellow-brown | few dark spots on posterior tail muscle* | \ |
|
Ophryophryne | |||||||
O. elfina | 25, N = 5 | visible | not visible | uniform brownish red or brownish orange | few round blackish spots on tail | pale brownish orange, intestine |
|
Pelobatrachus | |||||||
P. kalimantanensis | 30, and 36, N = 2 | visible* | not visible* | conspicuous dark brown and gold or orange brown pigmentation | marbled with dark brown pigmentation, edges of fins with golden iridophores | belly milky-white pigmented, pale stripe below spiracle extends laterally to half of abdomen* |
|
45, N = 1 | invisible* | not visible* | dark brown without orange gold pigmentation | dark brown | dark brown marbled pattern |
|
|
Xenophrys | |||||||
X. medogensis (low-elevation) | 35, and 38, N = 2 | \ | \ | pale yellow-brown | mottled with pale colored patches | without white patches |
|
X. medogensis (high-elevation) | 27, N = 1 | \ | \ | deep brown with copper pigmentation | brown, scattered with tiny white pigment spots, no dark brown patches on tail | semitransparent brown, covered with small white pigments |
|
X. cf. pachyproctus | 25, N = 1 | \ | \ | yellow-brown with two golden spots on dorsalateral mid body | \ | \ |
|
X. yeae | 28–29, and 31–35, N = 9 | \ | \ | brown with dense copper pigments | above lower fin mottled with copper patches | semi-transparent |
|
X. maosonensis | 25, N = 2 | obvious | visible | brown with dark brown speckles posteriorly | few dark brown speckles | speckled with metallic grey blue |
|
X. lekaguli | 25, 37–38, and 42, N = 6 | \ | \ | pale gray (in preservative) | proximal half of caudal muscle with two or three irregular dark streaks, fins distinctly pigmented only in distal portions (in preservative) | small black spots (in preservative) |
|
X. serchhipii | 32, 34, and 36–38, N = 11 | \ | visible | dark brown (in preservative) | translucent and grey (in preservative) | dark brown, fins are opaque and speckled (in preservative) |
|
X. monticola | 25, N = 5 | \ | \ | grey olive-green with irregular melanophores (in preservative) | densely arranged melanophores (in preservative) | immaculate, slightly translucent with some rare spots of melanophores (in preservative) |
|
X. periosa | 27, N = 1 | \ | \ | greyish brown | dense small speckles | translucent greyish brown |
|
34, N = 1 | \ | \ | greyish brown | large spots alongside anterior 2/3 of tail muscle | translucent greyish brown |
|
|
Incertae sedis with Megophryinae | |||||||
“Megophrys” dringi | 25, N = 4 | \ | visible | conspicuous pattern of intense dark brown and gold pigmentation | pigmented dark brown, interspersed with pale golden iridophores | milky translucent with a few irregularly shaped golden spots |
|
Comparison of morphological characteristics among tadpoles of the subfamily Megophryinae, which was identified based on molecular data. “*” indicates characteristics not mentioned in the text but were illustrated in the figure, and “\” indicates “no data”.
Species | Stage | N | TTL | BL | TAL/TTL | BW/ODW (expanded) | Mouthpart shape | Narial rim | Tail tip | References |
---|---|---|---|---|---|---|---|---|---|---|
Atympanophrys | ||||||||||
A. gigantica | 35 | 5 | 50.7 (42.6–54.9) | 16.9 (15.7–18.0) | 66.6 (63.2–68.4) | 62.6, N = 1 | hastate | serrated and raised | broadly rounded |
|
Brachytarsophrys | ||||||||||
Br. popei | 26–27 | 2 | 36.4±1.2 (35.0–37.3) | 8.5±0.8 (7.9–9.0) | 71.7(–) | 81.7±10.1 (74.6–88.9) | bi-triangular | raised | bluntly pointed | This study |
36–37 | 3 | 36.4±1.2 (35.0–37.3) | 10.8±0.3 (10.5–11.0) | 70.3±0.3 (70.0–70.5) | 77.2±1.4 (75.6–78.4) | bi-triangular | raised | bluntly pointed | This study | |
26–27 | 12 | \ | \ | \ | \ | \ | \ | bluntly pointed |
|
|
29 | 2 | \ | \ | \ | \ | \ | \ | bluntly pointed |
|
|
Br. intermedia | 32 | 2 | 45.0±4.7 (41.7–48.3) | 14.0±2.2 (12.4–15.5) | 69.7±1.7 (67.9–70.3) | \ | bi-triangular | raised | pointed |
|
36 | 1 | 48.7 | 15.0 | 69.2 | 50.6 | bi-triangular | raised | pointed |
|
|
39 | 1 | 55.1 | 16.3 | 70.4 | \ | bi-triangular | raised | pointed |
|
|
Br. orientalis | 36 | 1 | 33.9 | 12.3 | 69.2 | \ | \ | \ | pointed |
|
Boulenophrys | ||||||||||
Bo. fansipanensis | 25 | 2 | 30.8 (26.5–35.0) | 9.1 (7.4–10.8) | 69.1–72.1 | 64.8, N = 1 | bi-triangular | serrated and raised | pointed |
|
Bo. jingdongensis | 25 | 1 | 27.9 | 8.9 | 68.1 | 80.4, N = 1 | bi-triangular | serrated and raised | rounded |
|
Bo. hoanglienensis | 26 | 1 | 26.5 | 7.1 | 73.2 | 79.3, N = 1 | bi-triangular | serrated and raised | pointed |
|
Bo. shimentaina | 25–27 | 4 | 28.5±0.1 (28.5–28.6) | 7.9±0.4 (7.3–8.3) | 72.7±1.5 (71.6–74.4) | 72.3±5.8 (65.2–77.2) | bi-triangular | serrated and raised | bluntly pointed | This study |
28 | 1 | 27 | 8 | 70.4 | 68.3 | bi-triangular | serrated and raised | bluntly pointed | This study | |
Bo. cf. ombrophila | 25 | 1 | 20.9 | 5.1 | 75.6 | 69.2 | bi-triangular | serrated and raised | bluntly rounded | This study |
26–27 | 2 | 31.8±1.9 (30.4–33.1) | 8.7±0.4 (8.4–8.9) | 72.7±0.5 (72.4–73.1) | 62.7±1.7 (61.5–63.9) | bi-triangular | serrated and raised | sharply pointed | This study | |
36 | 1 | 33.7 | 10.0 | 70.3 | 61.4 | bi-triangular | serrated and raised | sharply pointed | This study | |
Bo. nanlingensis | 25–27 | 9 | 26.0±6.4 (18.7–39.1) | 7.1±1.5 (5.3–10.3) | 72.8±1.8 (70.8–76.1) | 71.4±4.9 (64.7–79.6) | bi-triangular | serrated and raised | pointed | This study |
28–29 | 3 | 30.1±4.8 (26.9–35.7) | 8.4±0.9 (7.7–9.4) | 71.8±2.1 (69.5–73.7) | 68.2±4.2 (63.3–71.2) | bi-triangular | serrated and raised | pointed | This study | |
34 | 1 | 34.4 | 8.8 | 74.4 | 85.7 | bi-triangular | serrated and raised | pointed | This study | |
35 | 1 | 40.1 | 10.8 | 73.1 | 73.1 | bi-triangular | serrated and raised | pointed | This study | |
Bo. lushuiensis | 26–27 | 3 | 27.8±4.0 (23.1–30.3) | 8.0±1.1 (6.8–8.8) | 70.2±1.9 (68.0–71.3) | 66.8±11.0 (56.1–78.0) | \ | \ | \ |
|
32 | 1 | 42.7 | 12.1 | 71.9 | 57.9 | bi-triangular* | \ | rounded* |
|
|
36 | 1 | 41.1 | 11.3 | 72.5 | 58.4 | \ | \ | \ |
|
|
Bo. rubrimera | 37 | 1 | 33.3 | 10.5 | 68.5 | \ | \ | \ | rounded |
|
Bo. baishanzuensis | 31 | 1 | 22.7 | \ | 64.8 | \ | bi-triangular* | \ | pointed |
|
Bo. leishanensis | 25–27 | 6 | 29.7±2.3 (27.0–33.0) | \ | 64.2±2.1 (61.5–66.7) | \ | \ | \ | pointed |
|
Bo. jiangi | 26 | 2 | 25.5–26.0 | \ | 65.5–70.4 | \ | bi-triangular* | \ | pointed |
|
Bo. lini | 28 | not provided | \ | \ | \ | \ | \ | raised | pointed |
|
31–34 | not provided | \ | \ | \ | \ | \ | raised | pointed |
|
|
Ophryophryne | ||||||||||
O. elfina | 25 | 5 | 28.4±1.3 (27.4–30.2) | 8.6±0.1 (8.4–8.7) | \ | \ | bi-triangular* | “nares tubular” | bluntly rounded |
|
Pelobatrachus | ||||||||||
P. kalimantanensis | 30 | 1 | 38.9 | 11.2 | 71.2 | \ | \ | \ | blunt |
|
36 | 1 | 47.0 | 12.9 | 72.6 | \ | \ | \ | blunt |
|
|
45 | 1 | 31.2 | 13.5 | 56.7 | \ | \ | \ | \ |
|
|
Xenophrys | ||||||||||
X. yeae | 28–29 | 4 | 34.3±0.4 (33.9–34.8) | 10.6±0.3 (10.2–11.0) | 69.0±1.2 (67.3–69.9) | 70.6±6.1 (64.8–78.0) | \ | \ | \ |
|
31–34 | 4 | 34.9±1.1 (33.7–35.8) | 11.0±0.5 (10.4–11.4) | 68.4±0.5 (68.0–69.1) | 78.6±13.7 (66.2–92.9) | \ | \ | \ |
|
|
35 | 1 | 38.4 | 10.9 | 71.6 | 66.2 | \ | \ | rounded* |
|
|
X. cf. pachyproctus | 25 | 1 | 19.1 | 6.1 | 68.1 | 63.3 | \ | \ | bluntly pointed* |
|
X. medogensis (high-elevation) | 27 | 1 | 33.7 | 9.5 | 71.5 | 98.1 | \ | \ | pointed* |
|
X. medogensis (low-middle elevation) | 35 | 1 | 42.7 | 13.3 | 68.9 | 85.2 | \ | \ | pointed* |
|
38 | 1 | 43.6 | 13.2 | 69.5 | 83.1 | \ | \ | \ |
|
|
X. maosonensis | 25 | 2 | 35.5 (34.4–36.6) | 8.8 (8.1–9.5) | 76.5–77.9 | 73.2 | bi-triangular | raised | narrowly rounded |
|
X. lekaguli | 25 | 2 | \ | 9.0–10.4 | \ | \ | \ | not raised | rounded |
|
37 | 2 | \ | 12.1–12.9 | \ | \ | \ | not raised | rounded |
|
|
38 | 1 | \ | 13.8 | \ | \ | \ | not raised | rounded |
|
|
42 | 1 | \ | 14.2 | \ | \ | \ | not raised | rounded |
|
|
X. serchhipii | 32 | 1 | 28.6 | 10 | 65.0 | \ | \ | \ | \ |
|
34 | 4 | 29.9±1.40 | 10.2±0.30 | \ | \ | \ | \ | \ |
|
|
36 | 4 | 29.3±0.47 | 11.3±0.11 | 72, N = 1 | \ | \ | “an elevated projection” | pointed |
|
|
37 | 1 | 28.9 | 11.9 | 58.8 | \ | \ | \ | \ |
|
|
38 | 1 | 35.6 | 13.0 | 63.5 | \ | \ | \ | \ |
|
|
X. monticola | 25 | 7 | 24.7±2.7 (21.1–28.1), N = 5 | 6.9±0.9 (5.9–8.2) | 70–71, N = 4 | \ | \ | “waves” | finely rounded |
|
X. periosa | 27 | 3 | 30.4±1.5 (29.0–32.0) | 8.9±0.1 (8.4–9.5) | 70.7±0.4 (70.3–71.0) | 60.3±3.6 (58.2–64.5) | bi-triangular* | \ | bluntly pointed |
|
34 | 3 | 47.3±4.4 (42.7–51.4) | 12.8±0.9 (12.1–13.8) | 72.9±1.1 (71.7–73.9) | 75.8±5.9 (69.9–81.6) | bi-triangular* | \ | bluntly pointed |
|
|
Incertae sedis with Megophryinae | ||||||||||
“Megophrys” dringi | 25 | 4 | 32.28±6.05 (23.23–37.63) | 9.11±1.89 (6.74–11.35) | 71±2 (69–73) | \ | \ | raised and projected | pointed* |
|
All tadpoles were collected from an artificial roadside drainage ditch (Fig.
The following description is based on five tadpoles at Stages 25–28 (N = 5). Body ratio ranges represent all specimens. Raw measurements are given in Table
CSUFT T10156 (Stage 25; Field voucher: MT06; GenBank accession number: ON209270) collected on 30 May 2021 from Tiantaishan (24.972277°N, 112.963394°E, ca. 1280 m a.s.l.), Mangshan, Hunan Province, China; and CSUFT T10277 (Stage 26, Field voucher: MT707; GenBank accession number ON209281), CSUFT T10279 (Stage 26; Field voucher: MT709; GenBank accession number: ON209264), CSUFT T10283 (Stage 28, Field voucher: MT713; GenBank accession number: ON209261); and CSUFT T10285 (Stage 27; Field voucher: MT715; GenBank accession number: ON209272) collected on 14 July 2021 from Xiangsikeng (24.937705°N, 112.990257°E, ca. 1530 m, a.s.l.), Mangshan, Hunan Province, China.
The body is oval and flattened above (BW/BL 51.3–55.0%, N = 5); the eyes are located dorsolaterally, and the pupils are round; the nares are oval, open laterally, closer to the eye than to the tip of the snout (NE/SN 62.5–71.4%, IND/IOD 67.6–71.9%, N = 5); the rims of nares are serrated, slightly raised from the body wall; the spiracle is sinistral, low on the left flank; the spiracle tube is short, free from the body at the tip and opens laterally (SS/BL 53.4–58.0%, N = 5); the anal tube opens medially, unattached to the ventral fin; the dorsal fin arises behind the body-tail junction while the ventral fin is connected to the trunk; the tail muscle is massive, taller than tail fins before reaching the 2/3 part of the tail length (TMH/MTH 50.0–55.6%, N = 5); the tail tip is bluntly pointed, the tail length accounts for 69.5–76.1% (N = 4) of the total length; the mouth is terminal and the oral disc is funnel-like (BW/ODW 65.2–77.2%, N = 5); four rows of oval submarginal papillae are visible on the upper lip, and five rows of oval submarginal papillae on the lower lip; keratodonts are absent; the upper jaw sheath is comb-like, exhibiting a small median notch; the lower jaw sheath is thin and sickle-shaped, weakly keratinized, and finely serrated.
The following description is based on a tadpole at Stage 27 (CSUFT T10285). In life, the background color of the body and tail is semi-transparent dark brown; the dorsum is pigmented pale brown which extends to the dorsal surface of anterior tail and gradually becomes golden; a distinct circled marking is present at the center of dorsum, forming a saddle with the background dark brownish coloration; the middle of the saddle is pigmented pale brown; and the neuromasts are distinctly visible. Laterally, the dorsal pattern extends to the region above the horizontal level of the spiracle on the trunk, and covers the whole lateral surface of head; the lateral surface of tail is pigmented brown; the tail and fins are covered with irregularly shaped pale golden spots, interspersed with dense dark brown speckles; the fins are semi-transparent; the anterior part of the dorsal fin is marbled with golden and dark brown speckles; the junction of the anterior half of the dorsal fin and the caudal muscle is pigmented dark brown, forming an incomplete line; the anterior part of the ventral fin and the anal tube exhibit minimal dark brown pigmentation; the posterior part of tail and fins are pigmented with dense dark brown markings. The ventral body is semi-translucent grey, pigmented with dark brown chromocytes, and is covered with dense small, indistinct milky-white speckles; the gills and gut coils are visible through the ventral skin; two large, milky-white spots are present on each side of the ventrolateral surface of head-body connection and are followed by a cluster of smaller spots. The oral disc is translucent milky white; the lateral and middle wings are covered with orangish pigmentation; the tips of the wings and the middle of the upper lip exhibit dark brown pigmentation; the submarginal papillae on lips are dark brown, and the narial rims are pigmented beige. The eye sclera is silver with black dots; the iris is orange sprinkled with black dots; and the spiracle is translucent without pigmentation.
Variation of coloration in life. The other four tadpole specimens match most of the descriptions above. However, the dorsum pattern of a saddle is not clearly visible in CSUFT T10156 and the dorsum is almost uniform pale brown in CSUFT T10177. The ventrolateral spots on head-body connection are very large in CSUFT T10283 (Stage 28, Fig.
Boulenophrys shimentaina tadpoles A–C freshly dead tadpole CSUFT T10285 (Stage 27) lateral view, dorsal view, and ventral view D oral disc of tadpole CSUFT T10283 (Stage 28) in life E ventral pattern of tadpole CSUFT T10277 (Stage 26) in life; and F ventral pattern of CSUFT T10283 (Stage 28) in life. E and F share the same scale bar with A–C.
In preserved specimens, the pale brown pigmentation on the dorsal surfaces of the body and tail are still visible; the golden and orangish pigmentation fade to milky white; the white spots on each side of the ventrolateral surface of head-body connection become translucent; there is no orange pigmentation on the mouthparts, and prominent black pigmentation can be observed on the tail.
The two distinct, conspicuous ventrolateral spots on ventrolateral surface of head-body connection could distinguish the tadpoles of Bo. shimentaina from most Boulenophrys tadpoles, including Bo. fansipanensis, which have a single spot visible on each side, and Bo. rubrimera, Bo. hoanglienensis, Bo. jingdongensis, Bo. leishanensis, Bo. jiangi, and Bo. lushuiensis with no ventrolateral spots; the ventral pattern of indistinct, small speckles on belly could distinguish Bo. shimentaina tadpoles from Bo. lini, which have dense large speckles (see
Tadpoles of Bo. shimentaina could be distinguished from the syntopic Boulenophrys tadpoles in Mangshan (see below for the descriptions) by having a dark brown background coloration of body and tail (vs. pale brown in Bo. cf. ombrophila and Bo. nanlingensis), and a tail pattern of dense dark brown markings posteriorly (vs. several large brown spots along tail muscle in Bo. cf. ombrophila; and many brown speckles in Bo. nanlingensis). Further comparisons between Bo. shimentaina tadpoles and all megophryinid tadpoles identified based on molecular data are shown in Tables
A single tadpole at Stage 25 was collected on 30 May 2021, together with the tadpoles of Bo. nanlingensis and Br. popei from the road ditch (Fig.
The following description is based on four tadpoles at Stages 25–27 (N = 3) and Stage 36 (N = 1). Body ratio ranges represent all specimens except where specified. Raw measurements are given in Table
CSUFT T10992 (Stage 25; field voucher: MT02; GenBank accession number: ON209283) collected on 3 June 2021; and CSUFT T10281 (Stage 26; field voucher: MT718; GenBank accession number: ON209275), CSUFT 10270 (Stage 36, field voucher: MT710; GenBank accession number: ON209267), and CSUFT T10272 (Stage 27, field voucher: MT712; GenBank accession number: ON209269) collected on 14 July 2021. All specimens were collected from Xiangsikeng (24.937705°N, 112.990257°E, ca. 1530 m, a.s.l.), Mangshan, Hunan Province, China.
The body is flattened and oval (BW/BL 52.9–54.8% at Stages 25–27, N = 3; and 51.0% at Stage 36, N = 1); the eyes are located dorsolaterally, the pupils are round; the nares are oval, opening laterally, closer to the eye than to the tip of the snout (NE/SN 60.0–73.3% at Stages 25–27, N = 3; and 82.4% at Stage 36, N = 1); the internarial distance is smaller than interorbital distance (IND/IOD 65.8–68.2% at Stages 25–27, N = 3; and 66.7% at Stage 36, N = 1); the rims of nares are serrated, slightly raised from the body wall; the spiracle is sinistral, low on the left flank, and opens posterolaterally; the spiracle tube protrudes posteriorly, free from the body at the tip (SS/BL 54.9–59.6% at Stages 25–27, N = 3; and 56.0% at Stage 36, N = 1); the anal tube opens medially, unattached to the ventral fin; the dorsal fin arises behind the body-tail junction while the ventral fin is connected to the trunk; the tail muscle is massive, taller than tail fins until reaching 2/3 of the tail length (TMH/MTH 45.8–53.3% at Stages 25–27, N = 3; and 48.3% at Stage 36, N = 1); the tail tip is usually sharply pointed (bluntly rounded in one small-sized specimen CSUFT T10992, Stage 25, TTL 20.9 mm); the tail length accounts for 72.4–75.6% of the total length at Stages 25–27 (N = 3), and 70.3% at Stage 36 (N = 1); the mouth is terminal and the oral disc is funnel-like (BW/ODW 61.5–69.2% at Stages 25–27, N = 3; and 61.4% at Stage 36, N = 1); three and four rows of short oval submarginal papillae are present on the upper and lower lips, respectively; keratodonts are absent; the upper jaw sheath is comb-like, exhibiting a small median notch, whereas the lower jaw sheath is thin and sickle-shaped, weakly keratinized, and finely serrated.
The following description is based on a tadpole at Stage 27 (CSUFT T10272, Fig.
Boulenophrys cf. ombrophila tadpoles A–C tadpole CSUFT T10272 (Stage 27) lateral view, dorsal view, and ventral view in life D oral disc of tadpole CSUFT T10270 (Stage 36) in preservative E ventral pattern of tadpole CSUFT T10281 (Stage 26) in life; and F ventral pattern of tadpole CSUFT T10270 (Stage 36) in life. E and F share the same scale bar with A–C.
Variation of coloration in life. Among the remaining three specimens examined, two tadpoles at Stages 26 and 36 match most of the coloration pattern of the description for CSUFT T10272 above (see Fig.
In preserved specimens, a fading of the dorsal pattern is observed; the golden spots on the lateral surfaces of the tail are not visible; the large spots on the anterior corner of the spiracle and gills become translucent, and the orange pigmentation on the lips disappears.
The tadpoles of Bo. cf. ombrophila differ from the syntopic tadpoles of Bo. shimentaina by the semi-translucent beige background coloration of body and tail (vs. dark brown), a ventral pattern of dense milky white speckles on belly (vs. indistinct small speckles), and the pattern on tail of large spots along tail muscle (vs. dense markings posteriorly); from Bo. nanlingensis (see below for tadpole description) by the ventral pattern of dense whitish speckles (vs. sparse speckles), and the pattern on tail of several large spots (vs. many speckles).
Compared to other described Boulenophrys tadpoles where species identification is supported with molecular data, the tadpoles of Bo. cf. ombrophila differs by the ventral pattern of dense whitish speckles (vs. relatively sparse metallic blue speckles in Bo. fansipanensis; sparse whitish speckles in Bo. jingdongensis; sparse metallic grey blue speckles in Bo. hoanglienensis; and scattered with silver tiny patches in Bo. lushuiensis), and the tail pattern of several large spots along tail muscle (vs. few dark brown speckles in Bo. fansipanensis; absence of large spots in Bo. jingdongensis; many dark brown speckles in Bo. hoanglienensis; small black spots in Bo. baishanzuensis; few dark spots on posterior tail muscle in Bo. jiangi; small black spots on tail muscle in Bo. leishanensis; and dozens of small dark brown patches in Bo. lushuiensis). Further comparisons between Bo. cf. ombrophila tadpoles and all megophryinid tadpoles that were identified based on molecular data are shown in Tables
One observed breeding site of Bo. cf. ombrophila was a relatively broad wetland crossed by a small shallow creek. Several water sources from the gentle slope of the bamboo forest fed this creek and made the entire area very wet. This breeding site was muddy, covered with leaf litter and fallen logs. The creek was narrow and slow flowing with maximum depth of 0.2 m. Some fallen logs blocked the creek and created still water areas. Only male Bo. cf. ombrophila and Q. exilispinosa were observed calling in this site during our visits from May to August, and in November. The potential predator of these frogs, an aquatic snake Opisthotropis cheni Zhao, 1999 which was observed once, in July, in this creek. A single small-sized tadpole specimen (CSUFT T10992, TTL 20.9 mm) at Stage 25 was collected from this site while the male frogs were heard calling before a heavy rainstorm on 3 June 2021 at 19:30 h at dusk. Three tadpoles were collected from the rocky area (Fig.
The following description is based on 14 tadpoles at Stages 25–29 (N = 12), and 34–35 (N = 2). Body ratio ranges represent all specimens except where specified. Raw measurements are given in Table
CSUFT T10144 (Stage 25; field voucher: MT04; GenBank accession number: ON209279) collected on 30 May 2021; and CSUFT T10302 (Stage 25, field voucher: MT722; GenBank accession number: ON209280), and CSUFT T10303 (Stage 25, field voucher MT723; GenBank accession number: ON209277) collected on 19 July 2021 from Tiantaishan (24.972277°N, 112.963394°E, ca. 1280 m a.s.l.); CSUFT T10261 (Stage 25; field voucher: MT701; GenBank accession number: ON209263), CSUFT T10262 (Stage 25; field voucher: MT702; GenBank accession number: ON209268), CSUFT T10273 (Stage 28, field voucher: MT703, GenBank accession number: ON209278), CSUFT T10991 (Stage 27; field voucher: MT711; GenBank accession number: ON209265), and CSUFT T10284 (Stage 25, field voucher: MT714; GenBank accession number: ON209271) collected on 14 July 2021; and CSUFT T10986 (Stage 35, field voucher: MT1106; GenBank accession number: ON209285) and CSUFT T10969 (Stage 34, field voucher: MT1109; GenBank accession number: ON209274) collected on 19 November, 2021 from Xiangsikeng (24.937705°N, 112.990257°E, ca. 1530 m, a.s.l.); and CSUFT T10376 (Stage 27, field voucher: MT756; GenBank accession number: ON209273), CSUFT T10377 (Stage 27, field voucher: MT757; GenBank accession number: ON209262), CSUFT T10378 (Stage 28, field voucher: MT758; GenBank accession number: ON209282), and CSUFT T10379 (Stage 29, field voucher: MT769; GenBank accession number: ON209266) collected on 28 July 2021 from Guizizhai (24.952750°N, 112.960470°E, ca. 1210 m a.s.l.), Mangshan, Hunan Province, China.
The body is elongated, oval, and flattened above (BW/BL 51.2–60.4% at Stages 25–29, N = 11; and 52.8–54.5% at Stages 34–35, N = 2); the eyes are located dorsolaterally, and the pupils are round; the nares are oval, closer to the eye than to the tip of the snout (NE/SN 55.6–80.0% at Stages 25–29, N = 12; and 57.9–62.5% at Stages 34–35, N = 2); the internarial distance is smaller than interorbital distance (IND/IOD 61.5–71.0% at Stages 25–29, N = 12; and 68.1–69.2% at Stages 34–35, N = 2); the nares open laterally; the rims of nares are serrated, slightly raised from the body wall; the spiracle is sinistral, low on the left flank, and opens posteriorly; the spiracle tube is short and slightly protrudes posteriorly (SS/BL 54.8–62.7% at Stages 25–29, N = 12; and 59.3–63.6% at Stages 34–35, N = 2). The anal tube opens medially and is unattached to the ventral fin; the dorsal fin arises behind the body-tail junction, and the ventral fin is connected to the trunk. The tail muscle is massive, deeper than tail fins before reaching the maximum tail height (TMH/MTH 43.4–63.0% at Stages 25–29, N = 11; and 50.7–54.5% at Stages 34–35, N = 2); the tail tip is pointed, the tail length accounts for 69.5–76.1% (at Stages 25–29, N = 11) and 73.1–74.4% (at Stages 34–35, N = 2) of the total length; the mouth is terminal and the oral disc is funnel-like (BW/ODW 63.3–79.6% at Stages 25–29, N = 12; and 73.1–85.7% at Stages 34–35, N = 2); four and five rows of short oval submarginal papillae can be observed on the upper and lower lips, respectively; keratodonts are absent; the upper jaw sheath is comb-like, exhibiting a weak median notch; the lower jaw sheath is thin and sickle-shaped, weakly keratinized, and finely serrated.
The following description is based on a tadpole at Stage 25 (CSUFT T10303, Fig.
Boulenophrys nanlingensis tadpoles in life A–C tadpole CSUFT T10303 (Stage 25) lateral view, dorsal view, and ventral view D ventral pattern of tadpole CSUFT T10262 (Stage 25) E ventral pattern of tadpole CSUFT T10273 (Stage 28); and F oral disc of tadpole CSUFT T10261 (Stage 25). D and E share the same scale bar with A–C.
Megophryinid tadpoles and their habitats in Mangshan, Hunan Province, China (A) Non-collected Bo. shimentaina tadpole (middle) feeding together with two unrecognized tadpoles in its habitat (B); and tadpole habitat (C) of a mixed-species assemblage beside a forest road, with unrecognized tadpoles feeding together (D).
Variation of coloration in life. The dorsal pattern coloration in tadpoles of Bo. nanlingensis is subject to significant variation both in same stages and between stages. At Stage 25, a small-sized tadpole (CSUFT T10144, TTL 18.7 mm) exhibits a yellowish dorsum with pale orange blotches, and dark brown pigmentation present posteriorly; another small tadpole (CSUFT T10284, TTL 18.9 mm) displays a brown dorsum with whitish patterns on the dorsolateral surfaces of the trunk and these extend to the tip of the tail. The coloration of the medium-sized tadpoles at Stage 25 (CSUFT T10261, TTL 25.1 mm; and CSUFT T10262, TTL 27.2 mm) and a broken-tailed individual at Stage 25 (CSUFT T10302) correspond to the dorsal pattern of CSUFT T10303 described above. However, the shape and coverage of dark brown markings varies between individuals. At later Stages 27–29, three medium-sized tadpoles (CSUFT 10377, Stage 27, TTL 28.1 mm; CSUFT T10376, Stage 27, TTL 24.8 mm; and CSUFT 10378, Stage 28, TTL 26.9 mm) exhibit a bi-colored dorsum, which is anteriorly pale brown and posteriorly inconspicuous dark brown. Tadpoles with relatively larger size at both early Stages 27–28 and advanced Stages 34–35 (CSUFT T10273, Stage 28, TTL 35.7 mm; CSUFT T10991, Stage 27, TTL 39.1 mm; CSUFT T10986, Stage 35, TTL 40.1 mm; and CSUFT T10969, Stage 34, TTL 34.4 mm) exhibit a uniform brownish dorsum coloration with almost invisible markings. Two tadpoles, CSUFT T10144 (Stage 25, TTL 18.7 mm) and CSUFT T10379 (Stage 29, TTL 27.8 mm) exhibit pale yellowish dorsum with orange pigmentation, which are indistinguishable from the small-sized Bo. cf. ombrophila tadpole (CSUFT T10992, TTL 20.9 mm). A tadpole at Stage 27 (CSUFT T10376, TTL 24.8 mm) with a mid-vertical line on dorsum is similar with that of larger Bo. cf. ombrophila tadpoles (Stages 26–27, and 36; TTL 30.4–33.7 mm). However, they were not collected form the same site as Bo. cf. ombrophila tadpoles. A large-sized tadpole at Stage 35 (CSUFT 10986, TTL 40.1 mm) showed a ventral pattern of large spots on belly that was different with other specimens. For tadpoles at Stages 34–35 (CSUFT T10969, and CSUFT T10986), the hindlimbs are semi-transparent, the outer aspect of the legs is pigmented yellow and interspersed with brown chromocytes on top.
In preserved specimens, a fading of the dorsal pattern is observed; the tail is translucent with sparse dark-brown pigmentation; the orange pigmentation on lips is no longer visible; the whitish speckle on the ventral surface and the nares are translucent.
The variation of dorsum pattern makes the tadpoles of Bo. nanlingensis are sometimes confused with the syntopic tadpoles of Bo. cf. ombrophila. Usually, the ventral pattern of sparse speckles (vs. dense speckles) and the tail pattern of many small speckles (vs. large spots) could distinguish them. An exception is the small-sized tadpole CSUFT 10144 (Stage 25, TTL 18.7 mm), which bears almost the same pattern as a small-sized Bo. cf. ombrophila tadpole CSUFT T10992 (Stage 25, TTL 20.9 mm). The tadpoles of Bo. nanlingensis differ from the syntopic Bo. shimentaina tadpoles by the pale brownish background coloration of the body and tail (vs. dark brown), the ventral pattern of sparse speckles (vs. dense small speckles), and the tail pattern of small dots (vs. large speckles).
Compared to other described Boulenophrys tadpoles where species identification is supported by molecular data, the tadpoles of Bo. nanlingensis differs by the presence of ventrolateral spots on each side of head-body connection (vs. absent in Bo. jingdongensis, Bo. hoanglienensis, Bo. leishanensis, and Bo. lushuiensis); the tail pattern of many brown speckles (vs. small spots on tail muscle in Bo. leishanensis; few dark spots on posterior tail muscle in Bo. jiangi; and small white and black dots in Bo. baishanzuensis). Further comparisons between Bo. nanlingensis tadpoles and all megophryinid tadpoles that were identified based on molecular data are shown in Tables
Tadpoles of Bo. nanlingensis were discovered in all collection sites during our field surveys in 2021, which perhaps implies that this species has larger population size, or it might exhibit less microhabitat specificity. Besides the three sites mentioned above, four Bo. nanlingensis tadpoles were collected from a relatively wide stream (3–5 m wide), with a maximum depth of 0.5 m. An adult male was observed calling under rocks near the stream bank with its feet standing in shallow water on 28 July 2021.
The male calling activities of Bo. nanlingensis, which began in late July, had increased during our visit in November in Mangshan. It seems the newborn larva would have to over-winter. Thus, we suspected the tadpoles of early Stages 25–29 collected in May and July were born in the previous year. Two tadpoles at advanced Stages 34–35 were collected on the 19th of November. Considering tadpoles in late stages develop relatively fast (Grosjean, 2003; TQ, personal observation). It was likely these advanced tadpoles would finish metamorphosis in cold season at the beginning of the next year. However, this assumption needs further confirmation because the cold weather and scarce food in winter may not be suitable for the survival of froglets.
In this study, we attempted to identify sympatric megophryinid tadpoles using external morphology and color patterns, especially ventral patterns. However, our sample size was small. Tadpoles of Bo. shimentaina and Bo. cf. ombrophila bearing stable and distinct ventral pattern were collected from a single site. It is not clear if the color pattern may differ between sites. In Bo. nanlingensis, the color pattern varied between different body size ranges rather than stages or collection sites. This provides new insight into that the coloration pattern perhaps should be classified by body size ranges in megophyinid tadpoles, and not only development stages. Diagnostic larval characters for delineating megophryid genera are still insufficient except for the ventrolateral pattern in Brachytarsophrys. However, there are characters shared within genera, such as the rim of nares is “tubular” in Ophryophryne elfina (Poyarkov et al. 2017), and O. microstoma Boulenger, 1903 (tadpoles described by Grosjean, 2003 without molecular data), but this rim is “serrated” in Boulenophrys tadpoles, described both in this study and in
We failed to find any Xenophrys mangshanensis adult or larva during our field surveys, despite Mangshan being its type locality. However, it was reported to be in sympatry with Bo. nanlingensis and Br. popei in Guangdong Province (
The presence of Bo. kuatunensis and Bo. brachykolos in Mangshan was not confirmed in this study, which agrees with the conjecture proposed by
We would like to thank Yuanlingbo Shang and Yao Luo for help collecting specimens; Guoxing Deng for assistance during field surveys. We thank Chaosheng Mu for the help in collecting literature. TQ thanks Zhengyan Zhou for the guidance in tadpole photography, and Yu Zhang for the help in tadpole staging. We are sincerely grateful to Annemarie Ohler, Benjamin Tapley, and an anonymous reviewer for their helpful comments and suggestions.
This work was supported by the project for Endangered Wildlife Protection of Hunan Forestry Bureau of China (No. HNYB-202201), the National Natural Science Foundation of China (No. 31472021), and the project for Endangered Wildlife Investigation, Supervision and Industry Regulation of the National Forestry and Grassland Bureau of China (No. 2021072-HN-001).