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Live specimens of Hirudinaria species A dorsal and B ventral views of H. manillensis from Sakon Nakhon Province C dorsal and D ventral views of H. bpling from Satun Province E dorsal and F ventral views of H. thailandica sp. nov. MUMNH-HIR008-28 (holotype) from Chai Nat Province. Scale bar: 10 mm.
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External morphology of the holotype of Hirudinaria thailandica sp. nov. in preserved state A dorsal and B ventral views of the whole body C dorsal views of somites XIX–XXIII D ventral views of somites X–XV E dorsal and F ventral views of somites I–VIII G dorsal and H ventral views of somites XXIV–XXVII and caudal sucker. Abbreviations: an, anus; fg, female gonopore; mg, male gonopore; np, nephridiopores; and ss, sensillae. Scale bar: 10 mm (A, B), 5 mm (C–H).
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Illustration of reproductive system of the holotype of Hirudinaria thailandica sp. nov. A dorsal view of male and female reproductive organs including positions of ganglia XII and XIII of the ventral nervous system. Lateral views of B male and C female reproductive organs. Scale bar: 2 mm. Abbreviations: atr, male atrium; cod, common oviduct; ej, ejaculatory bulb; ep, epididymis; fg, female gonopore; mg, male gonopore; ov, ovary; ps, penis sheath; ts, testisac. vas, vas deferens; vc, vaginal caecum; and vg, vagina.
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Phylogenetic analysis of Hirudinaria species and related taxa A bayesian inference tree based on 2533 bp alignment dataset of nuclear 28S rRNA and mitochondrial COI genes. Numbers at nodes indicate bootstrap values from maximum likelihood (ML) and bpp from Bayesian inference analysis (BI). Nodes with black circles are sufficiently supported by both BI and ML, while nodes with grey circles are supported only by ML B map showing the sampling locations for the specimens used for DNA sequence analysis.