Research Article |
Corresponding author: James Whitfield ( jwhitfie@life.illinois.edu ) Academic editor: Jose Fernandez-Triana
© 2021 Geraldo Salgado-Neto, Consuelo Alexandra Narváez Vásquez, Dillon S. Max, James Whitfield.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Salgado-Neto G, Vásquez CAN, Max DS, Whitfield JB (2021) Cotesia cassina sp. nov. from southwestern Colombia: a new gregarious microgastrine wasp (Hymenoptera, Braconidae) reared from the pest species Opsiphanes cassina Felder & Felder (Lepidoptera, Nymphalidae) feeding on Elaeis oil palm trees (Arecaceae). ZooKeys 1061: 11-22. https://doi.org/10.3897/zookeys.1061.67458
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A new species of microgastrine wasp, Cotesia cassina Salgado-Neto, Vásquez & Whitfield, sp. nov., is described from southwestern Colombia in Tumaco, Nariño. This species is a koinobiont gregarious larval endoparasitoid, and spins a common mass of cocoons underneath the host caterpillars of Opsiphanes cassina (Felder & Felder) (Lepidoptera, Nymphalidae), feeding on oil palm trees (interspecific hybrid Elaeis oleifera × E. guineensis) (Arecaceae). While superficially similar, both morphologically and biologically, to C. invirae Salgado-Neto & Whitfield from southern Brazil, the two species are distinct based on DNA barcodes, host species, geographical range and morphological characters.
Butterfly, DNA barcode, integrative taxonomy, morphology, natural enemy, new species
The nymphalid butterfly Opsiphanes cassina Felder & Felder occurs from Mexico to the Amazon Basin (Brazil, Bolivia, Colombia, Ecuador, French Guiana, Guyana, Peru, Suriname and Venezuela) (
Five species of Braconidae have been recorded as endoparasitoids of species of Opsiphanes (larval stage): Cotesia biezankoi (Blanchard), Cotesia opsiphanis (Schrottky), Cotesia alia (Muesebeck) (
Cotesia is easily recognizable morphologically among microgastrine braconids, although the huge variety of species can be difficult to distinguish from each other (
As Cotesia species appear to be highly host specialized (
Using such an integrative taxonomic approach, this paper provides a description of a new species of Cotesia, whose brood was produced from caterpillars of Opsiphanes cassina (Felder & Felder) (Lepidoptera, Nymphalidae) (Fig.
A simplified map of Colombia, showing rough location of Tumaco B close-up of southwestern Colombia, with location of Tumaco highlighted C caterpillar of Opsiphanes cassina on frond of the palm Elaeis oleifera × E. guineensis D same as C but with cocoons of emerged Cotesia cassina arranged below (normally underneath caterpillar).
Between April 2018 and March 2019; we collected 35 larvae of Opsiphanes cassina as part of a survey carried out on exotic palms in the Palmeiras plantation A.S., 58 km from San Andrés de Tumaco, Nariño, Colombia (1°47'28.0"N, 78°47'33.9"W, 28 m elev. – see Fig.
Photographs of the caterpillar and parasitoid cocoons (Fig.
Cotesia cassina, sp. nov. A lateral habitus B dorsal view of mesosoma and anterior metasomal tergites C fore wing D lateral view of metasoma with hind leg removed, showing laterotergites, sternites, hypopygium and ovipositor sheaths E frontal view of head F dorsal view of posterior portions of mesosoma, especially propodeum.
To characterize and compare the new species at the molecular level, the mitochondrial (DNA barcode) gene cytochrome oxidase I (COI) was analyzed. For the amplification of a fragment of approximately 460 bp of this gene, we used the following primer pair: COI-F (5’-GATTTTTTGGKCAYCCMGAAG-3’) and COI-R (5’CRAATACRGCTCCTATWGATAAWAC-3’) (
Holotype
Female, Colombia: Nariño, San Andrés de Tumaco (1°47'28.0"N, 78°47'33.9"W, 28 m elev.), March 2019, coll. Consuelo Vásquez, ex larva Opsiphanes cassina Felder & Felder (Lepidoptera, Nymphalidae). Deposited in the collection of the National University of Colombia (UNC, Dr Fernando Fernandez, curator). Paratypes 2 males, deposited in UNC, same data as holotype. 1 female, also same data as holotype, deposited in the Illinois Natural History Survey (
Diagnostic morphological characters distinguishing Cotesia cassina sp. nov. from the Brazilian C. invirae Salgado-Neto & Whitfield.
Character | C. invirae | C. cassina |
---|---|---|
Color | Generally lighter. T3 and all tergites posterior to T3 mostly bright yellow orangish. Mesopleuron with some light yellow/brown on ventral side | Generally darker. T3 and all tergites posterior to T3 are more brown to black rather than orangish. Mesopleuron almost entirely black |
T2 Sculpture | Mostly smooth. Sculpture is more uniform across width; less punctate laterally | More punctate laterally, smooth medially |
T2 Shape | Posterior margin/groove straight | Posterior margin slightly convex apically, with length greatest medially |
As discussed above, Cotesia is a huge worldwide genus of hundreds of species, with many morphologically similar species. While useful world identification keys are not available, it is currently possible to successfully diagnose species regionally, especially combined with molecular and host data. The closest described species, morphologically, biologically and within the region, is Cotesia invirae from southern Brazil, which also parasitizes Opsiphanes on palms (different species). The table below provides a diagnostic comparison between the two species.
Cotesia alia (Muesebeck), also recorded from Opsiphanes, resembles these two species but has a relatively longer first metasomal tergite (see illustration in
Female. Body length 3.1–3.3 mm; fore wing length 2.9–3.1 mm. Coloration (Fig.
Male. Similar to female except with slightly narrower metasoma.
COI barcode deposited in GenBank (MW405620). Using the identification tools in the Barcode of Life Database (
Opsiphanes cassina (Felder & Felder) (Lepidoptera, Nymphalidae) (Fig.
Cotesia cassina is a gregarious parasitoid wasp that occurs mainly in the wet season (March-May); however, their host, O. cassina, occurs throughout the year, mainly in the rainy season (March-July). Cotesia cassina larvae kill the host larva before the end of the last instar and form their cocoons in a regular mass of dirty whitish cocoons, regularly arranged disposed under the host (Fig.
Known so far from San Andrés de Tumaco, Nariño, Colombia (Neotropical Region).
The specific epithet cassina, is a reference to Opsiphanes cassina (Felder & Felder) (Lepidoptera, Nymphalidae), the host caterpillar name. The word cassina is the feminine of cassino which in Italian means playhouse.
We are grateful to Dr Ricardo Harakava of Instituto Biológico de São Paulo for analyses of the DNA barcode, and to Joshua C. Gibson at the University of Illinois for assistance with the wasp photography.