Research Article |
Corresponding author: Edwin Javier Quintero-Gutiérrez ( ejquinterog@gmail.com ) Academic editor: Farid Faraji
© 2020 Edwin Javier Quintero-Gutiérrez, Dorothee Sandmann, Orlando Cómbita-Heredia, Bernhard Klarner, Rahayu Widyastuti, Stefan Scheu.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Quintero-Gutierrez EJ, Sandmann D, Cómbita-Heredia O, Klarner B, Widyastuti R, Scheu S (2020) Review of the mite genus Krantzolaspina Datta & Bhattacharjee (Mesostigmata, Parholaspididae) with re-description of K. angustatus comb. nov. (Ishikawa) from Indonesia. ZooKeys 997: 47-68. https://doi.org/10.3897/zookeys.997.54262
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Herein, we update the diagnosis and description of the genus Krantzolaspina Datta & Bhattacharjee and provide a list of the three valid species including new combinations and synonyms, as follows: 1) Krantzolaspina angustatus (Ishikawa, 1987) comb. nov. (= Indutolaelaps jiroftensis
Gamasina, monotype, morphology, oriental region, rainforest, tropical lowland
The mesostigmatic mite family Parholaspididae has a total of 14 known genera distributed worldwide (
Indonesia is a biodiversity hotspot (
Despite the remarkable faunal diversity of the oriental region and numerous reported species of parholaspidid mites, there are shortcomings in the old species descriptions (lack of leg chaetotaxy, information on external poroidotaxy and adenotaxy of the idiosoma). For the previous, we decided to add information and organised the monotypic genus Krantzolaspina, based on available specimens, photographs from paratypes, original description and illustrations. For this reason, in the present work, a re-description of Krantzolaspina angustatus comb. nov. based on holotype, review of the generic concept based on type material and literature, a new synonymy and a list of valid species with some comments is presented.
This study forms part of an investigation on soil and canopy arthropods of rainforests and agricultural systems in Jambi Province, Sumatra, Indonesia and was conducted within the framework of the interdisciplinary project “Ecological and socioeconomic functions of tropical lowland rainforest transformation systems (Sumatra, Indonesia)” - EFForTS. For more details on the study region and the experimental design of the project see
Mites were extracted from soil of oil palm plantations in the vicinity of Bukit Duabelas National Park, Jambi Province, Sumatra, Indonesia. Soil samples (1–3 cm depth) were taken using a spade and each consisted of a core of 16 × 16 cm area. Mites were extracted from samples using the high gradient canister method described in
Photographs and measurements were made using an Axiolab 5 phase contrast Zeiss microscope with an Axiocam 105 HD digital camera and Nikon Eclipse Ci connected to a computer-controlled digital camera Sight Ds-L3. Stacks of images were taken for each mite, using manual control of the focal plane. Selected images were combined using Zerene Stacker, version 1.04 (Zerene Systems, LLC 2009-2014). In some cases, images captured from different regions of the body were combined using the ‘photomerge’ function in Adobe Photoshop, version 2015 (16.0 or 20150529.r.88; Adobe Systems Inc., San Jose, USA). Digital drawings were prepared with Adobe Illustrator, version CC 2015 (19.0.0), based on (combined) photographs.
All measurements are given in micrometres (μm) and include the range (minimum–maximum). Lengths of shields were measured along their midlines and widths at their widest point, except for the sternometasternal shield which was measured at the level of insertion of setae st2 and genitiventrianal shield between bases of JV1–2. Leg measurements were taken from the proximal margin of the coxa, along the midline of each segment, to the tip of the claw. Notations of body structures and idiosomal chaetotaxy follow
Specimens of examined K. angustatus comb. nov. are deposited at NSMT (National Science Museum Natural History), Tokyo, Japan, the Holotype and voucher specimens at
The updated diagnosis and description of the genus were prepared after consulting the original description of the genus Krantzolaspina (
Krantzolaspina Datta & Bhattacharjee, 1989: 411.
Krantzolaspina rebatii Datta & Bhattacharjee, 1989.
Female. Dorsal shield entire, usually bearing 32–36 pairs of setae. Presternal area with two pairs of free presternal platelets. Sternometasternal shield well-defined, bearing four pairs of setae. Genitiventrianal shield, bearing four pairs of setae: one pair of genital setae st5 and three pairs of preanal setae (Zv1 and Jv1–2) in addition to circumanal setae. Podal-peritrematal shield free from genitiventrianal shield. Epistome with long median projection and with lateral margins serrate. Cheliceral movable digit with a pair of unequal plumose arthrodial brushes and no arthrodial corona or with a single plumose arthrodial brush and a fringed arthrodial corona. Palp trochanter with a small spur-like process near its ventral base (not palpfemur as in the original description); palptarsal claw three-tined. Pretarsus I reduced or absent, pretarsi II–IV well-developed. Chaetotaxy of femur-genu-tibia of legs I: 13-12-12; II: 11-11-10; III: 6-8-8; IV: 6-8-8.
Male. Unknown.
Female. Dorsal idiosoma. Dorsal shield 340–561 μm long, broad anteriorly, gradually tapering posteriorly, anterior margin almost straight/truncate, partially covering idiosoma, leaving with the lateral and posterior margins of the soft integument broadly or narrowly exposed; shield mostly reticulate. Dorsal shield hypotrichous, bearing 32 (J5 and S5 slightly pilose in K. angustatus comb. nov.) or 36 pairs of smooth setae, most setae long and of similar length. Unsclerotised cuticle with 10–15 pairs of r, R and/or UR setae combined, smooth and moderately long (UR setae slightly pilose in K. angustatus).
Ventral idiosoma. All setae aciculate, smooth and relatively long (except Jv4–5, Zv4–5 pilose in K. angustatus). Tritosternum with a pair of free pilose laciniae. Presternal area with two pairs of free, well-sclerotised presternal platelets. Sternometasternal shield well defined, longer than wide. Shield with posterior and anterior margin concave, partially smooth, reticulate or punctate (anterior and lateral margins ornamented in K. angustatus), bearing four pairs of setae st1–4 and three pairs of poroids iv1–3. Peritrematal shield well-developed, broad, anteriorly free and posteriorly fused with developed podal shield; two pairs of poroids (id7, ip) and one pair of gland pores (gdp) posterior to stigma; Genitiventrianal shield longer than wide, reticulate, flask-shaped; cribrum developed. Soft opisthogastric cuticle with 6–7 pairs of setae; never hypertrichous. Metapodal platelets present or absent.
Gnathosoma. Subcapitulum with the corniculi well sclerotised, elongated and horn-like. Internal malae bifurcate, well separated from each other, densely fimbriated on outer margin and with apices slightly shorter than corniculi. Deutosternal groove with multidentate transverse rows, subcapitular setae smooth and aciculate, h1–h3 often longer than h2 and pc. Surface posterolaterad to seta pc with a pointed spine-like process or absent (simply flat). Epistome with a median projection and lateral margins irregularly serrate or may be smooth. Chelicera chelate-dentate; movable digit often bearing two teeth. A setiform pilus dentilus; smooth dorsal cheliceral seta, dorsal lyrifissure, a pair of unequal plumose arthrodial brushes or one plumose arthrodial brush and a narrow fringed arthrodial corona at base of the movable digit are present. Palp trochanter with a small pointed spine-like process in the ventral surface and with setae v1 and v2 slightly thickened and long, in contrast with setae of the other palp segments; palp tarsal claw three-tined.
Legs chaetotaxy as in diagnosis (see above).
Male. Unknown.
Proparholaspulus angustatus
– Ishikawa, 1987a: 82;
Indutolaelaps jiroftensis
. Female. Dorsal shield entire, mostly reticulate (except the anteromedial region which is smooth), partially covering the idiosoma, broad anteriorly and posteriorly narrowing; shield bearing 32 pairs of setae, most setae moderately long and smooth, except j1–2 and z1 which are slightly shorter and setae S5 and Z5 slightly pilose. Unsclerotised lateral cuticle of the idiosoma with a total of 15 pairs of smooth setae of similar length, except five pairs of UR setae slightly pilose, the pairs of setae r6 and R1 slightly shorter than r5–7 plus seven pairs of UR setae. In the idiosoma ventre, all setae aciculate and smooth, except JV4–5 and ZV4–5 which are pilose. Presternal area with two pairs of free and presternal platelets. Sternometasternal shield mostly reticulate and covered by semi-rounded cells in the lateral margins, bearing four pairs of setae. Peritrematal shield anteriorly free, fused to the sternometasternal shield between coxae I–III, posteriorly fused with the parapodal shield and extended beyond posterior margin of the coxae IV, region of peritrematal + parapodal shield reticulate and covered by some semi-rounded cells; peritreme extending between coxae I–II at level of st1. Genitiventrianal shield longer than wide, reticulate, flask-shaped and bearing four pairs of setae st5, JV1–3, as well as three circumanal setae pa and po. Soft opisthogastric ventral cuticle with six pairs of setae JV4–5, ZV2–5. Metapodal platelets free, small and elliptical in shape. Deutosternal groove with six transverse rows, surface of the gnathosoma with pointed spine-like process similar to the ventral surface of the palp trochanter. Epistome with median projection bifurcate or trifurcate distally, lateral edges finely and irregularly serrate. Cheliceral digit movable and fixed with four and two teeth, respectively, base of movable digit with a plumose arthrodial brush and a narrow fringed arthrodial corona. Palp tarsal claw three-tined. Pretarsus I reduced or absent and pretarsi II–IV with pretarsi well-developed.
Male. Unknown.
Female
(Figs
Idiosomal venter (Figs
Gnathosoma (Fig.
Krantzolaspina angustatus comb. nov., adult female, Gnathosomal structures. Holotype (Left); Indutolaelaps jiroftensis syn. nov. (Middle), photos by Raphael Castilho; New material from Indonesia (Right). A–C Subcapitulum D–F Epistome G–I Chelicera J–L Palp trochanter with a ventral spine-like process.
Legs (Fig.
Male. Unknown.
• 1♀ Holotype at NSMT (NSMT-Ac 9805) collected in Philippines, Palawan Islands, Olanguan Valley, between Puerto Princesa and Roxas, on litter of tropical forest. •16♀ vouchers at
K. angustatus comb. nov. significantly differs from K. rebatii and K. solimani comb. nov. in the following combination of characters: K. angustatus has 32 pairs of smooth dorsal setae (except S5 and Z5 slightly pilose), while K. rebatii and K. solimani have 36 pairs of smooth setae, respectively; K. angustatus has 15 pairs of setae r-R and UR-series in the unsclerotised lateral cuticle, whereas K. solimani and K. rebatii have 13 and 10, respectively; in K. angustatus and K. solimani, the peritreme is extended anteriorly between coxae I–II at the level of st1, while in K. rebatii, it is extended beyond coxa I; in K. angustatus, a spine-like process is developed in the subcapitulum, while this is absent in K. rebatii and K. solimani. Further, the arthrodial process in K. rebatii has a pair of long unequal arthrodial brushes, whereas the arthrodial process in K. solimani and K. angustatus has a relatively long arthrodial brush and a narrow fringed arthrodial corona. Additionally, in K. angustatus, small metapodal platelets elliptical in shape are present, while in K. solimani and K. rebatii, they are absent. Lastly, Table
Characteristics of the females of Krantzolaspina angustatus comb. nov., and some ambiguous or unknown data of K. rebatii and K. solimani comb. nov.
Characters | K. angustatus (Ishikawa, 1987) comb. nov. | *K. rebatii Datta & Bhattacharjee, 1989 | *K. solimani (Metwali, 1983) comb. nov. |
Dorsal shield ornamentation | mostly reticulate (smooth on the dorso-central region around bases of z1 and J2) | completely smooth? | with tetra- and pentagonal reticulation (except on dorsocentral region of setae j–J, faintly ornamented) |
Sternometasternal / ventrianal shield ornamentation | completely reticulate and with the lateral margins covered by some semi-rounded cells | mostly smooth but with lateral margins pointed?/ anterior margin pointed, elsewhere smooth? | mostly with tetra and pentagonal reticulation |
Parapodal-peritremal shield ornamentation | slightly reticulate + semi-rounded cells posterior to the coxa IV | completely smooth? | completely smooth? |
1 Setae of opisthogastric cuticle (JV–ZV) | Seven pairs (JV3–5, ZV2–5); JV4–5 and ZV4–5 pilose | Six pairs (JV4–5, ZV2–5)? | Six pairs (JV4–5, ZV2–5) |
Deutosternum: no. rows | Nine | three? | ? |
Leg chetotaxy | ? | ||
(Coxa – Tibia) | |||
I | 2, 6, 13, 12, 12, not counted | ?, ?, 10, 11, 11, ? | |
II | 2, 5, 11, 10, 10, 18 | ?, 4, 10, 9, ?, ? | |
III | 2, 5, 6, 8, 8, 18 | ?, 3, 5, 8, 7, ? | |
IV | 1, 5, 6, 8, 8, 18 | ?, ?, 4, 6, 7, ? |
Krantzolaspina is a well-defined genus by the unique combination of characters stated above (see diagnosis of the genus). However, a number of characters are also present in other dermanyssine families, for example, well-developed arthrodial brush(es) is/are also present in macrochelid mites, a sternometasternal shield bearing st1–st4 is shared with species of Pachylaelapidae (
Although the genitiventrianal shield is a diagnostic character for Krantzolaspina, it is not an exclusive character as it also occurs in other genera of Parholaspididae, such as Holaspulus, some species of Holaspina and Proparholaspulus, as well as in other families, such as Leptolaelapidae genus Indutolaelaps Karg, 1997. In addition, this feature occurs in some species of Laelapidae (see above), but in those species, the genitiventrianal shield is hyper-developed posteriorly and occupies most of the opisthogaster and is often named the hologastric shield (
One particular feature of Krantzolaspina is the pointed spur-like process present on the palp trochanter and this character is important for recognising the genus. However, a similar process is present in species (and all post-embryonic stages) of the monotypic family Megalolaelapidae (Megalolaelaps), in which the palp trochanter typically bears a large anteroventral horn-like projection. Unfortunately, the function of these processes of the palp trochanter in Krantzolaspina and Megalolaelaps is unknown (
Krantzolaspina species have been collected from soils, mainly disturbed soil and in countries of Southern Asia. However, its distribution is isolated since they are present in Iran, Philippines and recently recorded in Indonesia (see details of locality type and other records, Table
This species was described in the genus Indutolaelaps Karg, 1997 (Leptolaelapidae), based on a genitiventrianal shield, an epistome with an anteriomedial extension, wider at the base and acute distally and a palp tarsal claw three-tined (
Morphological characteristics of females in the genera Krantzolaspina and Indutolaelaps.
Morphological characters | *Krantzolaspina Datta & Bhattacharjee, 1989 (Parholaspididae) | **Indutolaelaps Karg, 1997 (Leptolaelapidae) |
---|---|---|
Arthrodial process | with two arthrodial brush or one arthrodial brush and one narrow fringed arthrodial corona | one fringed arthrodial corona |
Presternal platelelets | two pairs | one pair |
Number of preanal setae in the genitiventrianal shield | three pairs | five pairs |
Number of dorsal setae | 32–34 pairs | 50–55 pairs |
Gnathotectum | with a median projection slightly bifurcate, trifurcate or serrate distally and with lateral margins serrate | with a median projection, distally and lateral margins smooth |
Deutosternal rows | six, nine | five |
Cheliceral dentition | FD usually with four teeth (rarely three) and MD bidentate | FD with three teeth and MD bidentate |
Pretarsi | pretarsus I reduced or absent, pretarsi II–IV well developed | Pretarsus I reduced, pretarsi II–IV well developed |
Further, the synonymy of I. jiroftensis is supported in that it has 32 pairs of dorsal setae, two pairs of presternal platelets, sternometasternal and genitiventrianal shield with four pairs, as well a distinct ornamentation pattern (
Krantzolaspina rebatii Datta & Bhattacharjee, 1989: 411
The holotype of K. rebatii was deposited according to
Focusing on the original description, we like to mention some discrepancies and/or mistakes that we found between the text and illustrations as follows:
(1) Dorsal shield: “36 pairs of setae” is indicated in the text (
(2) Venter: The ornamentation seems to be excluded from their original text, but it looks mostly smooth in fig. 1a of
(3) Legs:
Neoparholaspulus solimani Metwali, 1983: 459.
In conclusion, the genus Krantzolaspina currently includes three valid species Krantzolaspina angustatus (Ishikawa, 1987) comb. nov., K. rebatii Datta & Bhattacharjee, 1989 and K. solimani (Metwali, 1983) comb. nov. Lastly, despite the valuable work undertaken by
Thanks to Bruce Halliday (CSIRO, Canberra, Australia) for help with literature and comments on Krantzolaspina solimani comb. nov. Thanks to Ken-ichi Okumura (NSMT, Tokyo, Japan) for providing the holotype of Krantzolaspina angustatus nov. com. NSMT-Ac 9805. Thanks to Raphael Castilho (UNESP, São Paulo, Brazil) for the pictures on the paratypes and information on Indutolaelaps jiroftensis