Research Article |
Corresponding author: Wonchoel Lee ( wlee@hanyang.ac.kr ) Academic editor: Danielle Defaye
© 2019 Seunghan Lee, Ho Young Soh, Wonchoel Lee.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Lee S, Soh HY, Lee W (2019) A new species in the genus Acartia Dana, 1846 (Crustacea, Copepoda, Calanoida, Acartiidae) from the South Pacific coastal waters of Nadi Bay, Fiji. ZooKeys 893: 69-89. https://doi.org/10.3897/zookeys.893.38369
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A new species in the genus Acartia, Acartia nadiensis sp. nov., is described from Fijian coastal waters. This species belongs to the subgenus Odontacartia based on the following morphological features: presence of a rostral filaments, a pointed process on the last prosomite, a serrated terminal spine on female P5, and the absence of a protrusion on the basis of the male right P5. This new species can be differentiated from its congeners by the combination of the absence of a spine on the first segment of the antennules, the short outer seta of female P5, and a medial spine on the exp-2 of the left male P5. Phylogenetic analyses using mitochondrial COI partial sequences show that the new species is distinct from its congeners.
Odontacartia, planktonic copepod, mtCOI, taxonomy, South Pacific
The genus Acartia Dana, 1846 is one of the most dominant groups of planktonic copepods and has a worldwide distribution in estuarine, coastal, and even oceanic waters (
During a survey of the diversity of planktonic copepods in Fijian waters, we collected an undescribed species of the genus Acartia that clearly belongs to the subgenus Odontacartia. In this study, we describe the morphological characters of the new species from Nadi Bay, Fiji. Partial mtCOI sequences were also obtained and compared with related species to determine if this new species is also genetically distinct from its congeners.
Specimens were collected from Nadi Bay, Fiji, using a 100 μm mesh plankton net having a 30 cm diameter mouth, and then preserved in 99% ethanol. Specimens were dissected in lactic acid, and mounted on slides with lactophenol. Preparations were sealed with transparent nail varnish. All drawings were prepared using a drawing tube attached to an Olympus BX51 differential interference contrast microscope. For scanning electron microscope (SEM) preparation, specimens were dehydrated in a series of graded ethanol solutions, then placed in isoamyl acetate, critical point dried, mounted on stubs, coated in platinum, and observed under a Hitachi S4700 field-emission electron microscope at Eulji University, Seoul, Korea. Descriptive terminology was adopted from
For DNA extraction, ethanol was removed from fixed specimens (99% EtOH) by washing with distilled water, and DNA was extracted using a tissue DNA purification kit (COSMO GENETECH, Co. Ltd, Korea). DNA was extracted from individual specimens. mtCOI DNA was amplified in 20 μl reaction volumes containing extracted tissue DNA and primers LCO-1490 (5'-GGT CAA CAA ATC ATA AAG ATA AAG ATA TTG G-3') and HCO-2198 (5'-TAA ACT TCA GGG TGA CCA AAA AAT CA-3') (
Sequences were aligned and edited using CLUSTAL W (
Species | Locality | GenBank no. | References |
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A. (Odontacartia) erythraea | Mokpo, Korea | MN603769–MN603773 | Present study |
A. (Odontacartia) japonica | Okinawa, Japan | MN603774 | Present study |
A. (Odontacartia) nadiensis | Nadi Bay, Fiji | MN603766–MN603768 | Present study |
A. (Odontacartia) ohtsukai | Busan, Korea | MN603775–MN603777 | Present study |
A. (Odontacartia) pacifica | Nakajima Island, Japan | KC287267 | Bucklin and Blanco-Bercial 2014 |
Nakajima Island, Japan | DQ071177 |
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A. (Odontacartia) spinicauda | Xiamen waters, China | DQ665253–DQ665254 | Liu et al. 2006 |
A. (Acartiura) omorii | Gwangyang Bay, Korea | MN603778 | Present study |
Genus Acartia Dana, 1846
Subgenus Odontacartia Steurer, 1915
Coastal water (17°45.848'S, 177°22.348'E), Nadi Bay, Fiji.
All specimens have been deposited in the Marine Biodiversity Institute of Korea (MABIK). Holotype 1♀ (MABIK CR00246502) and Allotype 1♂ (MABIK CR00246503) undissected and preserved in 70% ethanol. Paratype: 2♀♀ (MABIK CR00246504-CR00246505) dissected on 13 and 10 slides, respectively; 2♂♂ (MABIK CR00246506-CR00246507) dissected on 14 and 8 slides, respectively; 10♀♀ (MABIK CR00246508-CR00246517) and 4♂♂ (MABIK CR00246518-CR00246521) undissected and preserved in 70% ethanol. 4♀♀ and 4♂♂ dried, mounted on stub, and coated with platinum for SEM. All specimens are from the type locality and were collected by S. Lee on 10 October 2013. The illustrations are based on the paratypes (♀, MABIK CR00246504; ♂, MABIK CR00246506).
The specific name refers to the type locality of Nadi bay, Fiji.
Total body length 975–1050 μm (mean ± SD = 1018 ± 26 μm, n = 10, holotype 1015 μm) as measured from anterior margin of cephalosome to posterior margin of the caudal rami. Body surface armed with some sensillae (Fig.
Prosome 5-segmented (Fig.
Urosome 3-segmented (Figs
Antennule incompletely 18-segmented (Fig.
Antenna (Fig.
Mandible: (Fig.
Maxillule: (Fig.
Maxilla: (Fig.
Maxilliped (Fig.
Legs 1–4 (Fig.
P5 (Figs
Total body length 910–952 μm (mean ± SD = 931 ± 16 μm, n = 5, allotype 930 μm) measured from anterior margin of cephalosome to posterior margin of caudal rami. Body surface armed with some sensilla (Fig.
Prosome (Fig.
Urosome (Figs
Left antennule 22-segmented (Fig.
Other mouthparts and P1–P4 as in female. P5 asymmetrical (Fig.
Acartia nadiensis sp. nov. Scanning electron micrographs. A Female, rostrum B female, P5 C female, P5, terminal spine D female, genital double-somite E female, genital field F female, 1st urosomite, dorsal view G female, 2nd urosomite, lateral view H female, caudal rami, dorsal view. Scale bars: in μm.
Acartia nadiensis sp. nov. Scanning electron micrographs. A Female, urosome and caudal rami, ventral view B male, rostrum C male, 1st urosomite D male, 2nd–4th urosomite, dorsal view E male, 1st urosomite, lateral view F male, 2nd urosomite, lateral view G male, 5th urosomite and caudal rami, dorsal view H male, 4th urosomite and caudal rami, ventral view. Scale bars: in μm.
A 581 bp partial region of mtCOI was sequenced from five species: A. nadiensis sp. nov., A. erythraea, A. japonica, A. ohtsukai, and A. omorii. Sequences of two species (A. pacifica and A. spinicauda) were obtained from NCBI and also included in the analysis. All species belong to the subgenus Odontacartia except A. omorii, which belongs to the subgenus Acartiura and was used as the outgroup. The mtCOI sequences of A. nadiensis differed in a 24.1% from A. japonica, and in up to 29.0% from A. pacifica (Table
Phylogenetic tree based on mtCOI sequences (581 bp) of Odontacartia species including A. (Acartiura) omorii as outgroup. One-thousand bootstrap replicates were performed by MEGA6 using neighbor joining and minimum evolution methods. Neighbor joining bootstrap values shown above branches; minimum evolution bootstrap values are below branches.
Genetic variation within the subgenus Odontacartia species based on mtCOI sequence comparison including A. (Acartiura) omorii as outgroup.
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | |
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1. A. (Odontacartia) nadiensis 1 | ||||||||||||||||
2. A. (Odontacartia) nadiensis 2 | 0.000 | |||||||||||||||
3. A. (Odontacartia) nadiensis 3 | 0.000 | 0.000 | ||||||||||||||
4. A. (Odontacartia) erythraea 1 | 0.247 | 0.247 | 0.247 | |||||||||||||
5. A. (Odontacartia) erythraea 2 | 0.247 | 0.247 | 0.247 | 0.000 | ||||||||||||
6. A. (Odontacartia) erythraea 3 | 0.247 | 0.247 | 0.247 | 0.000 | 0.000 | |||||||||||
7. A. (Odontacartia) erythraea 4 | 0.247 | 0.247 | 0.247 | 0.000 | 0.000 | 0.000 | ||||||||||
8. A. (Odontacartia) erythraea 5 | 0.247 | 0.247 | 0.247 | 0.000 | 0.000 | 0.000 | 0.000 | |||||||||
9. A. (Odontacartia) japonica 1 | 0.241 | 0.241 | 0.241 | 0.244 | 0.244 | 0.244 | 0.244 | 0.244 | ||||||||
10. A. (Odontacartia) ohtsukai 1 | 0.277 | 0.277 | 0.277 | 0.258 | 0.258 | 0.258 | 0.258 | 0.258 | 0.264 | |||||||
11. A. (Odontacartia) ohtsukai 2 | 0.275 | 0.275 | 0.275 | 0.266 | 0.266 | 0.266 | 0.266 | 0.266 | 0.269 | 0.028 | ||||||
12. A. (Odontacartia) ohtsukai 3 | 0.278 | 0.278 | 0.278 | 0.260 | 0.260 | 0.260 | 0.260 | 0.260 | 0.267 | 0.002 | 0.030 | |||||
13. A. (Odontacartia) pacifica 1 | 0.288 | 0.288 | 0.288 | 0.282 | 0.282 | 0.282 | 0.282 | 0.282 | 0.267 | 0.249 | 0.249 | 0.252 | ||||
14. A. (Odontacartia) pacifica 2 | 0.290 | 0.290 | 0.290 | 0.287 | 0.287 | 0.287 | 0.287 | 0.287 | 0.278 | 0.254 | 0.254 | 0.257 | 0.009 | |||
15. A. (Odontacartia) spinicauda 1 | 0.266 | 0.266 | 0.266 | 0.254 | 0.254 | 0.254 | 0.254 | 0.254 | 0.292 | 0.183 | 0.203 | 0.181 | 0.252 | 0.262 | ||
16. A. (Odontacartia) spinicauda 2 | 0.263 | 0.263 | 0.263 | 0.259 | 0.259 | 0.259 | 0.259 | 0.259 | 0.287 | 0.183 | 0.203 | 0.181 | 0.247 | 0.257 | 0.003 | |
17. A. (Acartiura) omorii | 0.292 | 0.292 | 0.292 | 0.251 | 0.251 | 0.251 | 0.251 | 0.251 | 0.270 | 0.297 | 0.290 | 0.299 | 0.296 | 0.304 | 0.268 | 0.270 |
The new species, Acartia nadiensis sp. nov., clearly belongs to the subgenus Odontacartia. This subgenus displays the following diagnostic characters compared to the other five subgenera of Acartia: presence of rostral filaments, symmetrical pointed process on the posterior corner of the last prosomite, minutely serrated terminal spine on female P5, and absence of protrusion on the basis of male right P5 (
Morphological differences among species within the subgenus Odontacartia (Calanodia: Acartiidae: Acartia).
A. nadiensis sp. nov. | A. amboinensis | A. australis | A. bispinosa | A. bowmani | A. centura | A. edentata | A. erythraea | A. japonica | A. lilljeborgi | A. mertoni | A. ohtsukai | A. pacifica | A. spinicauda | |
Female | ||||||||||||||
Body length | 975–1050 | 1340–1510 | 1290–1400 | 1320–1530 | 1200–1300 | 1350–1400 | 1190–1230 | 1400 | 1350–1410 | 1330–1400 | ND | 1190–1230 | 1190–1210 | 1250 |
Spine on 1st seg of antennules | absent | present | present | present | absent | absent | absent | present | present | present | absent | absent | absent | absent |
P5 | ||||||||||||||
Basis length/width ratio | 2 | 4 | 2.5 | 2.4 | 1.5 | 1.6 | 1.4 | 2.3 | 2.1 | 1.4 | 2 | 1.8 | 1.4 | 1.4 |
Length ratio of P5 outer seta/terminal spine | 0.4 | 1.8 | 1.2 | 1.4 | 0.9 | 1.6 | 1.3 | 1.6 | 0.7 | 1.5 | 1 | 1 | 1.8 | 1.2 |
Urosome | ||||||||||||||
Dorsal spines on 1st urosomite | 4 | 2 | 2 | 2 | 0 | 2 | 0 | 2 | 2 | (small spinules) | 2 | 2 | 2 | 2 |
Dorsal spines on 2nd urosomite | 4 | 4 | 0 | 0 | 2 | 2 | 2 | 2 | (small spinules) | (small spinules) | 2 | 2 | 2 | 2 |
Caudal rami length/width ratio | 1.8 | 1.3 | 1.1 | 1.8 | 2 | 1.7 | 3 | 1.4 | 1.2 | 1.5 | 2 | 3 | 2.5 | 3 |
Male | ||||||||||||||
Body length | 910–952 | ND | 1170–1230 | 1070–1160 | 1100 | 1250–1280 | 1080–1150 | ND | 1190–1240 | 1100 | ND | 1030–1050 | 1030–1160 | ND |
Left P5 | ||||||||||||||
Length ratio of medial process/segment on 2nd exopodite | 0.5 | ND | 0.7 | 0.4 | 0.9 | 0.8 | 2 | 0.4 | 1 | 0.5 | 3.5 | 1.4 | 1.6 | 0.9 |
Type of medial process on 2nd exopodite | Spine with teeth | ND | Spine | Spine with fine setae | Spine | Spine | Long seta | Spine | Spine with teeth | Spine | Long seta | Long seta | Long seta | Spine |
References | This study |
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To supplement the morphological evidences, we conducted molecular phylogenetic analyses using partial mtCOI sequences of six Odontacartia species, including the new species. The mtCOI gene is widely used to identify sibling species due to its higher evolutionary rate than 16s and 18s rDNA (Knowlton and Weight 1998;
The length ratio of the outer seta/terminal spine of the female P5 is the most diagnostic morphological feature in Odontacartia species. However, this character is also used to determine the subgenus Euacartia (
1 | Presence of spine on 1st to 2nd segments of female antennule | 2 |
– | Absence of spine on 1st to 2nd segments of female antennule | 5 |
2 | Small spinule row present on dorsal surface of female 1st urosomite | A. lilljeborgi |
– | Strong spines present on dorsal surface of female 1st urosomite | 3 |
3 | Absence of processes (spines and spinules) on dorsal surface of female 2nd urosomite | 4 |
– | Small spinule row present on dorsal surface of female 2nd urosomite | A. japonica |
– | 2 strong spines present on dorsal surface of female 2nd urosomite | A. erythraea |
– | 4 strong spines present on dorsal surface of female 2nd urosomite | A. amboinensis |
4 | Length-width of female caudal rami are almost similar; medial process on 2nd exopodite of male left P5 as spine | A. australis |
– | Female caudal rami almost twice longer than wide; medial process on 2nd exopodite of male left P5 as spine with fine seta | A. bispinosa |
5 | Dorsal surface of female 1st urosomite devoid of processes (spines and spinules) | 6 |
– | Spine present on dorsal surface of female 1st urosomite | 7 |
6 | Female caudal rami twice longer than wide; medial process and 2nd exopodite segment of male left P5 almost similar in length | A. bowmani |
– | Female caudal rami three times longer than wide; medial process of male left P5 twice longer than 2nd exopodite segment | A. edentata |
7 | Dorsal surface of female 1st and 2nd urosomite with two strong spines | 8 |
– | Four strong spines on dorsal surface of female 1st and 2nd urosomite | A. nadiensis sp. nov. |
8 | Length of female P5 outer seta and terminal spine similar | 9 |
– | Female P5 outer seta is longer than terminal spine | 10 |
9 | Female caudal rami is twice as long as wide | A. mertoni |
– | Female caudal rami three times longer than wide | A. ohtsukai |
10 | Length-width ratio of female caudal rami as 1.7; medial process on 2nd exopodite male left P5 as spine | A. centura |
– | Length-width ratio of female caudal rami as 2.5; medial process on 2nd exopodite of male left P5 as long seta | A. pacifica |
– | Length-width ratio of female caudal rami as 3; medial process on the 2nd exopodite of male left P5 as spine | A. spinicauda |
We thank Dr Hyusu Yoo (Hanyang University) for helping to prepare SEM photos. This research was supported by the Marine Biodiversity from Overseas Program of the Marine Biodiversity Institute of Korea (MABIK) funded by the Ministry of Oceans and Fisheries (MOF). Wonchoel Lee was supported by a grant from the National Research Foundation of Korea (NRF, NRF grant no. 2018R1D1A1B07050117).