Research Article |
Corresponding author: M. Antonio Todaro ( antonio.todaro@unimore.it ) Academic editor: Pavel Stoev
© 2018 Matteo Dal Zotto, Francesca Leasi, M. Antonio Todaro.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Dal Zotto M, Leasi F, Todaro MA (2018) A new species of Turbanellidae (Gastrotricha, Macrodasyida) from Jamaica, with a key to species of Paraturbanella. ZooKeys 734: 105-119. https://doi.org/10.3897/zookeys.734.23023
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The study falls within the framework of a wider research programme aimed at investigating the gastrotrich diversity of the Tropical North-Western Atlantic (TNWA). A new macrodasyidan gastrotrich is described from fine-medium sand collected at Duncans Bay, Jamaica. The description is based on observations carried out on living specimens using differential interference contrast microscopy. Paraturbanella xaymacana sp. n., the third gastrotrich taxon reported from Jamaica, is a mid-sized species, up to 564 μm long, with a feeble peribuccal swelling. The most obvious autapomorphic traits pertain to the testes and the male pore, both of which are located approximately at mid body, rather than at- or near the pharyngo-intestinal junction as occur in the other species of the genus. Additional differences with congeners are discussed and a key to the Paraturbanella species is provided, in the hope it will be useful to both gastrotrich experts and marine ecologists who discover these microscopic metazoans during their research.
Benthos, biodiversity, Caribbean Sea, meiofauna, taxonomy
The biodiversity of microscopic organisms belonging to meiofauna is scarcely known compared to other metazoans. Knowledge is particularly scarce for the ‘minor phyla’, such as Kinorhyncha (
The present study is part of a larger research programme aimed at shedding light on the diversity and phylogeny of gastrotrich species of the Tropical North-Western Atlantic. From 2010 to 2013, several international groups of researchers surveyed the gastrotrich fauna of different islands in the South Floridian, Bahamian, Lesser Antilles and Central Caribbean ecoregions. Accounts of these studies can be found in, e.g.,
We describe here a new species of Paraturbanella from the northern shore of Jamaica. It shares the same position of the male gonads with a recently described species from South Africa. In addition, we propose a determination key to the species of the genus.
Sampling campaign took place in February 2011 and included 10 locations along the North and West coasts of Jamaica. The species described herein was found in samples collected by hand from the shallow sublittoral (- 0.5 m); about 1 L of sandy sediment was placed into 500 mL plastic jars (
The gastrotrich specimens of interest were picked out with a micro-pipette, mounted on glass slides in a drop of 7 % MgCl2 solution, and studied in vivo with Nomarski differential interference contrast optics using a Zeiss Axio Scope A1. Photographs and measurements were taken with a DS-5M Nikon digital camera and Nikon NIS-F software. The description of the new species follows the convention of
Abbreviations used in the text are as follows: PhIJ, pharyngo-intestinal junction; TbA, adhesive tubes of the anterior series; TbD, adhesive tubes of the dorsal series; TbDL, adhesive tubes of the dorsolateral series; TbL, adhesive tubes of the lateral series; TbP, adhesive tubes of the posterior series; TbV, adhesive tubes of the ventral series; TbVL, adhesive tubes of the ventrolateral series.
Granulometric analysis of the substrata was carried out according to
Frequency of a species within collected samples follows Hummon et al. (
In the identification key we consider as ventrolateral the adhesive tubes that in some instances have been called, by other authors, lateral tubes. Furthermore, we consider Paraturbanella dolichodema Hummon, 2010 furnished with dorsal adhesive tubes and lacking ventral adhesive tubes, contra the original description that indicated that dorsal tubes are absent and the ventral tubes are present (
The sediment samples were collected on 24 February 2011 from Duncans Bay, Duncans, Jamaica (18°29'13.05'N, 77°32'03.23"W).
Holotype: the 542 μm long adult specimen shown in Figures
Two adults (including the holotype) collected by MAT & FL from the type locality; specimens were observed alive and are no longer extant i.e., both physical specimens were inadvertently destroyed during the study. Considering the size and nature of these organisms, the provided drawings, and the original multiple photos of the studied animals, the establishment of a new species-group taxon should be considered valid under the recommendation 73G-J of Declaration 45 – Addition of Recommendations to Article 73 (
Sparse in frequency of occurrence (10 % of samples), scarce in abundance (3–5% of a sample); sub-littoral at a water depth of about 0.5 m in sediment made up of fine, moderately sorted carbonate sand (mean grain size, 0.18 mm; sorting 0.59; kurtosis, 2.52; skewness, 0.43). Values of salinity and temperature of the interstitial water at the time of sampling were 34 ‰ and 26 °C respectively.
Body strap-shaped, up to 564 μm in length. Head with a feeble peribuccal swelling, with a slight constriction at U3.7; pestle organs present. PhJIn at U31; body widest from mid-pharynx to mid-intestine, thinning gradually to the caudal base; caudum bilobed, incised from its tips to U95, with a clearly visible medial cone; distance between apices of outermost TbP on either side is 1.3 times the width of the caudal base. About 20–23 glands are distributed along both lateral body margins in a single column per side. TbA six per side, the innermost being the shortest, whereas the adjacent being the longest, occur on fleshy hands that insert at approximately U11; TbV, TbVL, TbL and TbD absent; TbP, six per side, occurring as 4, 1, 1, the outermost being the longest; caudal cone present; accessory adhesive tubes (called also dohrni/Seitenfüsschen) two per side, posterolaterally directed (longer tube = 21 μm, shorter tube = 14 μm), inserting ventrolaterally just behind the hands at U14. Locomotor ciliature runs from the TbA rearward in two longitudinal bands that trace the lateral body margins, joining after the anus. Mouth terminal, width narrow; buccal cavity medium-sized, mug-shaped; walls heavily cuticularized; pharyngeal pores near the base at U28; intestine straight, broadest in front; anus ventral at U91. Hermaphroditic, paired testes extend rearward from U51, with sperm ducts recurving to the fore at U63 and emptying to the exterior via a common pore at U49; paired ovaries, the largest ovum occurs in the mid-gut region at U51. Frontal organ dorsal to the intestine at U63.
The specific name alludes to the original name of Jamaica: Xaymaca, (adjective: xaymacana) an Arawak word meaning “land of wood and water”.
Mostly based on the adult holotype, 542 μm in total length. Body strap-shaped; head with a feeble peribuccal swelling and a slight constriction at U04 and then the body proper. Pestle organs, small, at U5; body widest at mid-intestine, thinning gradually to the caudal base; caudum bilobed, deeply incised from its tips to U95, with a visible medial cone; distance between apices of outermost TbP on either side is 1.3 times the width of the caudal base. Widths at outer oral opening/head constriction/mid-pharynx/PhJIn/mid-intestine/furcal base, and their locations along the body length are: 12/26/33/39/45/29 μm at U0/U04/U17/U31/U61/U95. Epidermal glands are in one column per side, scattered along the body margins, up to 20–23 and variable in size (4–7 μm in diameter).
Adhesive tubes.TbA, six per side (7–11 μm in length), all occurring on fleshy hands that insert at approximately U11; the innermost, mimicking a thumb, is the shortest, while the second from the inner side is the longest; TbV, TbVL,TbL, TbD absent; TbP, six per side, occurring as two groups of 4, 1, 1 elements each, along the inner (4 + 1 tube) and distal margin of each lobe (1 tube); the distal tube being the longest (14 μm in length) and the four proximal ones the shortest (6–7 μm in length); a caudal medial cone is present, but it is rather short, 4 μm in length. Accessory adhesive tubes (known also as dohrni tubes or Seitenfüsschen) two per side, posterolaterally directed (longer tube=21 μm, shorter=13.7 μm from their base), arise ventrolaterally just behind the fleshy hands at U14, usually being held close to the body.
Ciliation. Tufts of sparse cilia (11–21 μm in length) occur on lateral and dorsal sides of the head, behind the mouth. Additional sensory hairs, of similar length (13–19 μm), occur along the pharyngeal and intestinal region, organized in lateral, dorsolateral and dorsal columns, with about 20–23 hairs per column. Ventral locomotor cilia (16–20 μm in length) flow from the head constriction rearward in two longitudinal bands that trace the lateral body margins, and join behind the level of the anus.
Digestive tract. Mouth terminal, narrow (9 μm diameter); buccal cavity large, mug-shaped, 18 μm in length and approximately 11 μm in width, with walls heavily cuticularized; Pharynx 153 μm in length, with pharyngeal pores near the base at about U28; PhJIn at U31; intestine straight, broadest in front; anus ventral at U91.
Reproductive tract. Hermaphroditic; paired testes extend posteriorly from U51, with short sperm ducts recurving toward the front at U63, and emptying to the exterior via a common pore located at U49; ovaries paired, with the oocytes occurring from U64 to U68 and maturing from posterior to anterior; a large egg (approximately 70 by 24 μm) was present in the mid-gut region centred at U51. Caudal organ absent; frontal organ, vesicular, dorsal to the intestine centered at about U63; it is ovoid in shape (28 by 26 μm) and contains sparse spermatozoa and secretory material.
The other studied adult specimen was 564 μm in total body length, with 154 μm long pharynx. Number and arrangement of TbA, and of the TbP along the caudal lobes matched those of the holotype. The placement of the testes and the male pore is similar to that of the holotype. Unfortunately the animal got destroyed during the study so no further details could be acquired. The unfortunate event happened while we were trying to confirm the crossing of the ascended and descendent tracts of the sperm ducts observed in the holotype (see Figures
Prior to the current study there were 22 described species of Paraturbanella (
Line-art illustration of Paraturbanella xaymacana sp. n. A Habitus as seen from the ventral side B Habitus as seen from the dorsal side, showing the internal anatomy. Abbreviations: A anus AAT additional adhesive tubes (Seitenfüsschen) BC buccal cavity CC Caudal cone CL caudal lobe E egg EG epidermal gland FO frontal organ FPS Fleeble peribuccal swelling MP male pore Ph pharynx PhIJ pharyngo-intestinal junction PhP pharyngeal pore PO pestle organ Sd sperm duct SdC sperm duct crossing TbA anterior adhesive tubes TbP posterior adhesive tubes Te testis. Scale bar: 100 μm.
Paraturbanella xaymacana sp. n., holotype. Differential interference contrast photomicrographs. A Habitus, ventral view B Anterior region, ventral view, showing the buccal cavity (asterisk), the pharyngeal pores (arrowheads), and the pharyngo-intestinal junction (arrow) C Anterior region, ventral view, showing the lateral and ventral ciliation, the anterior adhesive tubes (arrowheads), and the additional adhesive tubes (Seitenfüsschen) (arrows) D Posterior region, ventral view, showing the medial cone (arrowhead) and the posterior adhesive tubes (arrows). Scale bars: 100 μm (A), 50 μm (B), 20 μm (C–D).
Paraturbanella xaymacana sp. n., holotype. Differential interference contrast photomicrographs. A Mid body, dorsal view, showing the testes (asterisks) beside a ripe egg, the sperm ducts (arrows), and the position of the male pore (arrowhead) B Mid body, dorsal view, showing the frontal organ (arrow) and a cluster of sperm (arrowhead). Scale bars: 20 μm (A–B).
Several taxonomic keys to species of Gastrotricha have been developed in the last two decades (e.g.
Taxon | Distribution |
---|---|
Paraturbanella africana | KwaZulu-Natal, South Africa |
Paraturbanella aggregotubulata | Florida, USA |
Paraturbanella armoricana | Bretagne, France |
Paraturbanella boadeni | Andaman, India |
Paraturbanella brevicaudata | Lakshadweep, India |
Paraturbanella cuanensis | Ireland and UK |
Paraturbanella dohrni | Throughout the North Sea and the Mediterranean Sea; Gulf and Atlantic* coast of Florida, US; Red Sea*; Somalia (as P. cf dohrni) |
Paraturbanella dolichodema | Pacific coast of the US |
Paraturbanella eireanna | North Ireland |
Paraturbanella intermedia | Washington State, US |
Paraturbanella levantia | East Mediterranean Sea |
Paraturbanella manxensis | Isle of Man, UK |
Paraturbanella mesoptera | Andhra Pradesh, India |
Paraturbanella pacifica | Galapagos Islands, Ecuador |
Paraturbanella pallida | Throughout the Mediterranean Sea; Isles of Scilly, UK; Hawaii*. |
Paraturbanella palpibara | Andhra Pradesh, India |
Paraturbanella pediballetor | British Isles; Normandy, France |
Paraturbanella sanjuanensis | Washington State, US |
Paraturbanella scanica | Norway |
Paraturbanella solitaria | Pacific coast of the US |
Paraturbanella stradbroki | Queensland, Australia; Hawaii* |
Paraturbanella teissieri | Throughout the North Sea and the Mediterranean Sea; Gulf and Atlantic coast of Florida, US |
1 | TbVL present | 2 |
– | TbVL absent | 10 |
2 | TbD present | 3 |
– | TbD absent | 8 |
3 | TbD and TbVL clustered in the mid trunk region | P. aggregotubulata Evans, 1992 |
– | TbD and TbVL uniformely distributed along the trunk region | 4 |
4 | TbP in a single row per side | 5 |
– | TbP in a double row per side | P. armoricana (Swedmark, 1954a) |
5 | Mouth protruding outwardly; testes just behind the PhIJ | 6 |
– | Mouth not protruding outwardly; testes at some distance from the PhIJ | 7 |
6 | Head slightly narrowing forward; pestle organs faint; caudal cone elongate | P. dolichodema Hummon, 2010 |
– | Head deeply narrowing forward; pestle organs prominent; caudal cone usually not presen | P. levantia Hummon, 2011 |
7 | Head slanted anteriorly; pestle organs and caudal cone absent | P. stradbroki Hochberg, 2002 |
– | Head not slanted anteriorly; pestle organs and caudal cone present | P. scanica Clausen, 1996 |
8 | TbP in a single row per side | 9 |
– | TbP in a double row per side | P. manxensis Hummon, 2008 |
9 | TbA, 5–6 per side; TbP, 7 per side | P. eiranna Maguire, 1976 |
– | TbA, 11–12 per side; TbP, 10–13 per side | P. intermedia Wieser, 1957 |
10 | Head with a peribuccal swelling | 11 |
– | Head without a peribuccal swelling | 17 |
11 | Head bearing anteriorly two pairs of club-shaped sensory palps | P. palpibara Rao & Ganapati, 1968 |
– | Head lacking sensory palps | 12 |
12 | Testes at or near the PhIJ | .13 |
– | Testes at or passed mid body | .16 |
13 | Head bearing ventral papillae | P. teissieri Swedmark, 1954b |
– | Head lacking ventral papillae | 14 |
14 | TbA, less than 8 per side | P. solitaria Todaro, 1995 |
– | TbA, 8 or more per side | 15 |
15 | TbP, 8 per side occurring in pairs; caudal cone elongate | P. sanjuanensis Hummon, 2010 |
– | TbP, 8–10 evenly spaced, caudal cone short | P. mesoptera Rao, 1970 |
16 | Peribuccal swelling noticeable; testes at mid body; TbA, 6 per side; TbP, 5 per side arranged as 3, 1, 1 elements | P. africana Todaro, Dal Zotto, Bownes & Perissinotto, 2017 |
– | peribuccal swelling feeble ; testes passed mid body; TbA, 6 per side; TbP, 6 per side arranged as 4, 1, 1 elements | P. xaymacana sp. n. |
17 | TbP in a single row per side | 18 |
– | TbP in a double row per side | 22 |
18 | Total body length > 860 µm; caudal cone absent | P. pediballetor Hummon, 2008 |
– | Total body length < 760 µm; caudal cone present | 19 |
19 | TbA inserted on the outer side of a cuticular rod | P. boadeni Rao & Ganapati, 1968 |
– | TbA inserted in hand-like fashion on a fleshy base | 20 |
20 | Pestle organs absent | P. cuanensis Maguire, 1976 |
– | Pestle organs present | 21 |
21 | Caudal lobes short and reduced; TbA, 6 per side; TbP, 5 per side | P. brevicaudata Rao, 1991 |
– | Caudal lobes normally developed; TbA, 5–6 per side; TbP, 5–8 per side | . P. dohrni Remane, 1927 |
22 | Total body length < 400 µm; pestle organs absent | P. pacifica Schmidt, 1974 |
– | Total body length > 600 µm; pestle organs present | P. pallida Luporini, Magagnini & Tongiorgi, 1971 |
This work was mainly supported by a grant from the US-National Science Foundation to R. Hochberg (n. DEB 0918499). Additional funding was obtained from the Italian MIUR: “Finanziamento annuale individuale delle attività base di ricerca” grant to M.A. Todaro. Many thanks are due to the staff of the Discovery Bay Marine Laboratory for the assistance received during FL and MAT stay in Jamaica. Authors are thankful to R. Hochberg and T. Kånneby for their constructive comments on an early draft of the manuscript.