Live male of Poecilia vivipara, UFRN 0225, 25.2 mm SL. Maceió River microbasin, Fernando de Noronha Archipelago, Pernambuco, Brazil.

Sampling site of Poecilia vivipara in the border of the mangrove at Maceió River microbasin, Fernando de Noronha Archipelago, Pernambuco, Brazil.

Map with the sampling sites of individuals used on genetic analyses. Circles represent sampled sites. Squares represent sequences retrieved from GenBank. An asterisk represents the type locality of Poecilia vivipara. Different colours represent different phylogenetic clades. Sampling sites: 1 Margarita island 2 Maceió 3 Jaguaribe 4 Piranhas-Açu 5 Potengi 6 Paraíba do Norte 7 Ipojuca 8 São Francisco 9 Piauí 10 São João 11 Paraná.

Rooted Bayesian phylogenetic reconstruction tree of Cytochrome Oxidase I mitochondrial gene of Poecilia vivipara. Number in nodes represents the value of posterior probability.

Haplotype network showing intraspecific relationships among Poecilia vivipara haplotypes. Empty circles represent non-sampled haplotypes. Thin bars on branches represent mutational steps.