First record of Rhoptrocentrus piceus Marshall (Hymenoptera, Braconidae, Doryctinae) as parasitoid of Psacothea hilaris hilaris (Pascoe) (Coleoptera, Cerambycidae)

Abstract The species Rhoptrocentrus piceus Marshall (Hymenoptera: Braconidae) was reared from the larvae of the xylophagous beetle Psacothea hilaris hilaris (Pascoe) (Coleoptera: Cerambycidae), an exotic pest of Ficus and Morus species native to eastern Asia. It was recorded in the north of Italy in September 2005. This discovery is the first report of this species as parasitoids of the yellow spotted longicorn beetle all over the world.


Introduction
The invasion of new exotic species is an increasing phenomenon in all European countries. One of the main reason is the great increase of import and export goods, including living plant material, throughout the world. Italy is particularly vulnerable due to the structure of its territory. It extends over a wide latitude, with numerous mountain ranges along its length. Such territory structure creates a great variability in micro-climatic conditions. The presence of many different ecosystems in such a rich geo-morphological and climatic context can facilitate the settlement of new invasive species (Frasconi et al. 2013). It has been estimated that approximately 200 exotic species have settled in Italy since 1970 and the highest number of new records, 111 new species, was registered in the decade 1991-2000 (Longo 2009, Jucker andLupi 2011).
The initial success of an exotic pest is due to the interaction of the biological performance of the species with habitat characteristics (Gröbler andLewis 2008, Jucker andLupi 2011). When an exotic pest colonizes a new habitat, native potential natural parasites need time to find, recognize, and adapt to the new host species.
Among the pests recently detected in Italy, there is the yellow spotted longicorn beetle Psacothea hilaris hilaris (Pascoe) (Coleoptera: Cerambycidae: Lamiinae: Lamiini), an exotic pest of Morus and Ficus trees. The insect is native to eastern Asia (Kim et al. 2009) and was detected in Europe for the first time in Italy in 2005, where it has now become established (Jucker et al. 2006, Lupi et al. 2013. In 2012 the beetle was also recorded in Germany (EPPO 2012). In the native countries the insect is mostly associated with mulberry trees, whereas in Italy it prefers fig trees (Lupi et al. 2013). The pest larvae tunnel into the xylem of host trees after a first period of feeding under the bark. This results in considerable damage to the tree, which is progressively weakened until death. The adults feed on the leaves and on the tender bark of the smaller branches. Studies of the biology in the native countries indicated that P. h. hilaris is generally univoltine, but depending on the time of oviposition, it could be also bivoltine (Watari et al. 2002).
As studies on natural enemies are few also in its native countries (Hong et al. 2008), a long-term study has been carried out to improve the knowledge on P. h. hilaris relationships with autochthonous natural enemies in Italy. The present paper reports the results of a survey that was carried out in an area where P. h. hilaris is present since 2006.
A visual analysis of the infested fig trees was first performed. The observation of sawdust was the evidence of the presence of P. h. hilaris preimaginal instars. Branches were cut from infested plants in different sites, transferred to the laboratory, and stored inside cages at room temperature. Some larvae were removed and checked with the stereomicroscope to confirm P. h. hilaris presence using the key proposed by Pennacchio et al. (2012). Cages were controlled weekly to check the presence of parasitoids and the emergence of P. h. hilaris or other bark beetles from the logs.
The emerged specimens of Hymenoptera were collected, stored as dry material as well as in alcohol (70%) and classified to species level following Marsh (1997) and Belokobylskii (2001).

Results and discussion
From the logs collected in one locality in Erba (Como municipalities) [45°48'06.78"N; 9°13'02"E] on 10 September 2013, 29 females (no males) emerged of Rhoptrocentrus piceus Marshall (Hymenoptera: Braconidae: Doryctinae): four specimens in November 2013 and the others in April 2014. In the same logs only adults of P. h. hilaris were registered.
The body length of R. piceus ranged from 2.75 mm to 4.86 mm, confirming the high variability of the body size for this species (Becker 1979, Belokobylskij 2001 ( Table 1). The main features of the genus and the species were confirmed by consulting the most recent keys (Marsh 1997, Belokobylskij 2001, Belokobylskij and Maetô 2009. Terminology adopted for morphological features and measurements follows Belokobylskij and Maetô (2009) (Figs 1 and 2).
The genus Rhoptrocentrus belongs to the tribe Doryctini including approximately 35 Palaearctic genera ). This is a moderately large subfamily of the family Braconidae with more than 1000 described species worldwide. Most of the known doryctine species are idiobiont gregarious ectoparasitoids of the larvae of xylophagous or bark-boring Coleoptera, while some species live on Lepidoptera or Hymenoptera-Symphyta (sawfly) larvae. Exceptionally they were reared from adults of Embiopter or living within termites nests and several Neotropical genera behave as phytophagous or gall-associated wasps (Marsh 1997, Zaldivar-Riveron et al. 2014. The genus Rhoptrocentrus includes only three described species, R. piceus Marshall with a Holarctic distribution (Yu et al. 2012), R. cleopatrae Belokobylskij, so far known only from Egypt (Belokobylskij 2001), and R. yarramanensis Belokobylskij, Iqbal et Austin, recently described from Australia . Rhoptrocentrus piceus is relatively common in the western Palaearctic, but in its eastern distribution, this species was recorded only from Japan (when it was secondarily described under the name Doryctomorpha chlorophori: Watanabe 1951), with large gaps of its distribution in the eastern part of Russia between the Urals and Japan. Interestingly, R. piceus again appeared in north Vietnam (first record: 1 female, "Vietnam: Hoa Binh Province, Yen This species has a wide range of hosts mainly belong to the orders of Coleoptera [families Anobiidae, Bostrichidae, Buprestidae, Cerambycidae, and Curculionidae (including Scolytinae)], but also to Hymenoptera (Xiphydriidae) and Lepidoptera (Coleophoridae and Tortricidae) (Belokobylskij and Maeto 2009, Belokobylskij and Žikić 2009, Yu et al. 2012, Žikić et al. 2013. The host range of this parasitoid, as well as its wide distribution across all the Holarctic region, with penetration into the Oriental region, suggest a high ability in adapting to different ecological conditions. All these characteristics make it a very suitable parasitoid for the containment of new woodboring invasive species representing an increasing problem across the Italian territories (Loni et al. 2012). Our finding of this wasp on the new exotic pest P. h. hilaris seems to validate such a consideration and encourages further studies regarding the biology of R. piceus as well as the possibility to mass rear it (Turgeons and Smith 2013).