First report of Melittobia australica Girault in Europe and new record of M. acasta (Walker) for Italy

Abstract Melittobia acasta and Melittobia australica are newly recorded from Sicily, Italy, and the second species is reported in Europe for the first time. A short historical background about Melittobia parasitoid wasps, their hosts, and distribution, with emphasis in those two species is presented together with illustrations to facilitate their identification. Brief discussion about the presence and possible distribution of the species in Sicily is also included.

Twelve species are known in the genus Matthews et al. 2009). All Melittobia species exhibit extreme intrasexual and intersexual polymorphism, where males are blind, and brachypterous, and have highly modified antennae. Females are either brachypterous, and emerge from the pupae containing a large batch of eggs and are ready to mate, or macropterous, and capable of greater dispersal (Cusumano et al. 2010;Matthews and González 2008;Matthews et al. 2009;Schmieder 1933). Of these, Melittobia acasta (Walker), has been known as the only Eurasian species of Melittobia but it occurs widely in other regions of the world (Summarized by González et al. 2004). It has been reported as a dangerous threat to honeybees and/or solitary bees used as crop pollinators (See González and Matthews 2005).
Melittobia australica Girault, was described on the basis of three males and ten females that emerged from Pison spinolae Shuckard (Hymenoptera: Sphecidae) (Girault 1912). This parasitoid is a nearly cosmopolitan species that has been reported from Australia and New Zealand, several countries of Africa, Asia, North, Central and South America, including a few Caribbean islands/countries (Assem et al. 1982;Dahms 1984a;González and Matthews 2005;González and Terán 1996;Maeta 1978).
Here we confirm the presence of two Melittobia species (M. australica and M. acasta) in Italy and provide the first record of M. australica from Europe.

Materials and methods
Collections of trap nests and mud dauber nests were carried out during 2010 in western Sicily. Trap nests were built by pooling together about 10 pieces (Ø=2-3 cm; length=20 cm) of reed (Arundo donax L.) that were hung on trees located both in cultivated and uncultivated fields. Nests of mud dauber wasps were collected mainly from external walls of buildings especially in areas close to water sources.
A number of Melittobia wasps emerged from trap nests colonized by Osmia bees originally placed in the Palermo University campus (38°06'26"N,13°21'07"E). The collected parasitoids were mounted and later identified.
One of several mud dauber nests constructed by Sceliphron spirifex L. (Hymenoptera: Sphecidae) collected in the town of Contessa Entellina (province of Palermo) (37°44'23"N, 13°08'27"E), had a cell containing a prepupae of its host parasitized with Melittobia wasps. Some were collected and placed on calliphorid pupae (Calliphora sp., Diptera: Calliphoridae) to be reared, while the rest were studied and identified. The parasitoids that emerged from these cultures were studied, identified and counted (Table 1).
A thorough review of literature was done in order to corroborate that the identified species indeed constituted a new record for the country. We were unable to review other insect collections besides the one at Universitá degli Studi di Palermo, and that of an amateur entomologist in Palermo. Voucher specimens of both parasitoid species and the hosts are deposited at the TAMU insect collection, College Station, Texas, USA and at Palermo University, Palermo, Sicily, Italy.

Results and discussion
Four males and 63 macropterous females of Melittobia acasta were collected (and mounted) from a cell of Osmia sp. inside a trap nest.
A total of one male and 376 females of M. australica emerged from a Sceliphron spirifex prepupa. It is worth noting that the factitious host Calliphora pupae appear to be highly nutritious and large allowing the development of brachypterous females (Table 1). From six cultures established using Calliphora pupae (four females/fly pupae) as hosts, fifteen males of M. australica, as well as six brachypterous females, 201 macropterous females and 305 pupae/larvae emerged (see Table 1).
The easiest way to separate Melittobia acasta and M. australica is by examining male specimens under a magnifying glass (10×-20×) or a microscope. Males of M. acasta are 1.3-1.5 mm long, light brown or amber in color ( Figure 1a); antennal scape has a cupshaped depression, the distal region of the scape is strongly oblique with a broad excavation (Figure 2a) (Dahms 1984a;González and Terán 1996). Males of M. australica are 1.1-1.3 mm long, light amber in color and have an antennal scape with a deep ventral longitudinal groove (Figures 1b, 2b) (Dahms 1984a;González and Terán 1996). In order to identify species using females we recommend following Dahms (1984a).
Even though the North American species Melittobia chalybii Ashmead was once mentioned in an European country (Denmark) by Holm (1960), it was later clarified that the species was actually M. acasta (Holm and Skou 1972;González and Matthews, 2005). Otherwise, all reports we had been able to find of the presence of Melittobia in European countries, indicate that the species found was always M. acasta (González and Matthews 2005;González et al. 2004). Thus it was not surprising that the first Melittobia we encountered in Sicily was M. acasta. Since the species was encountered in Palermo, a major port-city in the island, we might suspect that the species found its way to Sicily through the many ships that come from many lands to this place. Since M. acasta is widely distributed in Europe and Asia, we might even speculate that its invasion of Sicily could have occurred centuries ago. Even though Melittobia australica is a nearly cosmopolitan species, it has never been reported from Europe until now. The finding of this species in the town of Contessa Entellina, located about 80-90 km from the port of Palermo raises an interesting question: how long has it been in Sicily? Since the place where we found M. australica in Sicily is in the interior of the island, we might suspect that the species is widely distributed on the island. It is important to note that several species of bees and wasps known from Sicily (Incalterra et al. 2003;Rasmont et al. 2008;Schmid-Egger 2003) are suitable hosts for both Melittobia wasps found, helping them to easily establish on the island.