Research Article |
Corresponding author: Makoto Kato ( kato@zoo.zool.kyoto-u.ac.jp ) Academic editor: Christopher Majka
© 2023 Makoto Kato, Atsushi Kawakita.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Kato M, Kawakita A (2023) Diversity and larval leaf-mining habits of Japanese jewel beetles of the tribe Tracheini (Coleoptera, Buprestidae). ZooKeys 1156: 133-158. https://doi.org/10.3897/zookeys.1156.97768
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From the Japanese Archipelago, 12 Habroloma and 20 Trachys species (Buprestidae: Tracheini) have been recorded. Two new Habroloma species were found, which are associated with Elaeocarpaceae and Loranthaceae, also new host plant families/orders for Tracheini. The two new species are described as Habroloma elaeocarpusi sp. nov. and Habroloma taxillusi sp. nov., and the latter is the first Tracheini species shown to be associated with epiphytes. Leaf mines of 31 Tracheini species are also reported in this work, including new records of leaf mines for 16 Tracheini species. The larvae of all these recorded species are full-depth linear-blotch mesophyll miners of mature leaves and pupate within their mines. The mining habits of Habroloma species associated with Symplocos (Symplocaceae) are unique: the young larvae bore into midribs and petioles and cause leaf fall, and the larvae then mine the fallen leaves.
Agrilinae, Habroloma, leaf miner, mining pattern, Symplocos, Trachys
The coleopteran family Buprestidae is a species-rich clade whose larvae are xylophagous wood-borers, while the leaf-mining habit has evolved (
Leaf-mining habits in Buprestidae are believed to have evolved from wood-boring habits (
Of the 32 Japanese trachyine species, host plants have been reported for 28 (
To shed light on the diversity and host plant associations of trachyine species in Japan, we have conducted extensive rearing of leaf-mining larvae on diverse plants and a substantial collection of mined leaves. From the accumulated materials, we identified two undescribed trachyine species. The two new species are associated with two new plant orders and families for Tracheini: Oxalidales (Elaeocarpaceae) and Santalales (Loranthaceae). Furthermore, we detected leaf mines for 31 trachyine species, including new leaf mine records for 18 trachyine species. In this paper, we describe the two new species, as well as the leaf mines of 31 trachyine species, and discuss the diversity and evolution of plant utilization patterns of trachyine species in the Japanese Archipelago.
We have conducted extensive sampling of buprestid leaf mines from the Japanese Archipelago since the 1980s. By rearing the leaf-mining larvae, we obtained 400 adult buprestid beetles. All of the specimens were collected by MK unless otherwise noted. The leaves containing leaf mines were dried, and the dried herbarium specimens have been deposited in the
Kyoto University Museum (
The morphology of adult specimens was examined under a microscope (VHS-7000; Keyence). Specimens were photographed by synthesizing virtual images from a sequence of corresponding depth images. To observe male genitalia, the specimens were macerated in hot water and dissected under a microscope. The abdomen was removed from the body and then cleaned in 5% KOH solution for ~ 12 h at room temperature. After washing in distilled water, the terminalia extracted from the abdomen were mounted on slides with glycerol.
Among adult beetles that emerged from collected leaf mines, we identified two new Habroloma species. We describe the two species using the following key to Japanese species. In this key, Habroloma hikosanensis is missing because it is within the morphological variation of Habroloma yuasai.
1 | Pronotum with a large distinct fovea at the post-inferior side of each anterior angle | bifrons (Kiesenwetter, 1879) |
– | Pronotum with a shallow depression at the post-inferior side of each anterior angle | 2 |
2 | Ventral surface of body flattened; thickness index (body thickness/body length) less than 0.36 (Fig. |
3 |
– | Ventral surface of body convex ventrally; thickness index (body thickness/body length) greater than 0.37; prosternal process round or lingulate, longer than wide, posterior margin often rounded (Fig. |
6 |
3 | Elytra clothed with greyish golden hairs except silvery vitta (Fig. |
4 |
– | Elytra clothed with greyish hairs except silvery vitta (Fig. |
5 |
4 | Elytra with a distinct V-shaped silvery vitta (Fig. |
eximium (Lewis, 1892) [host: Symplocos] |
– | Elytra without a distinct V-shaped silvery vitta (Fig. |
liukiuensis Obenberger, 1940 [host: Symplocos] |
5 | Elytra with a V-shaped silvery vitta, which neighboring a V-shaped black vitta ahead (Fig. |
griseonigra (E. Saunders, 1873) [host: Quercus] |
– | Elytra with two waving transverse silvery vittae on posterior half; anterior vitta M-shaped (Fig. |
elaeocarpusi Kato, sp. nov. [host: Elaeocarpus] |
6 | Basal 2/3 of elytra with a steel-blue patch (Fig. |
lewisii (E. Saunders, 1873) [host: Rosa]. |
– | Elytra without steel-blue patch | 7 |
7 | Elytra with three wavy silvery and golden transverse vittae on posterior 2/3 (Fig. |
taxillusi Kato, sp. nov. [host: Taxillus] |
– | Elytra with two wavy silvery or golden transverse vittae on posterior half (Fig. |
8 |
8 | Body subovate, less attenuated posteriorly, margins nearly parallel in basal half (Fig. |
nixilla (Obenberger, 1929) [host: Lagerstroemia] |
– | Body cuneiform, attenuating toward posterior end even from basal half (Fig. |
9 |
9 | Anterior wavy transverse silverly/golden band of posterior elytra complete (Fig. |
10 |
– | Anterior wavy transverse silverly/golden band of posterior elytra disconnected midway (Fig. |
12 |
10 | Anterior wavy transverse band silvery (Fig. |
marginicolle (Fairmaire, 1888) [host: Rubus] |
– | Anterior wavy transverse band grayish-golden and partly silvery (Fig. |
11 |
11 | Elytra with the sides constricted behind humeri; prosternal process as long as wide | asahinai Y. Kurosawa, 1959 [host: Rubus] |
– | Elytra with the sides not constricted behind humeri (Fig. |
yuasai Y. Kurosawa, 1976 [host: Platycarya] |
12 | Elytra strongly attenuate from base to the apex, with the sides less arcuate and distinctly constricted behind humeri (Fig. |
subbicorne (Motschulsky, 1860) [host: Rubus] |
– | Elytra attenuate from the base to the apex, with the sides not constricted behind humeri (Fig. |
atronitidum (Gebhardt, 1929) [host: Rubus] |
Holotype : Japan: ♂ (MK-BP-a327), Mt. Osuzu, Tsuno-cho, Miyazaki Pref. (32.262°N, 131.471°E, 230 m above sea level), 14-VII-2021 (as larva on Elaeocarpus japonicus), emerged on 27-VII-2021, NSMT-I-C-200265.
Paratypes : Japan: 1♂(MK-BP-a360), same data as holotype, emerged on 30-VII-2021, NSMT-I-C-200266; 1♀ (MK-BP-k35), Isso, Yakushima-cho, Yaku Island (30.440°N, 130.472°E, 60 m above sea level), 11-VI-1993 (as larva on Elaeocarpus japonicus), emerged on 26-VI-1993, NSMT-I-C-200267.
Japan: 2♂2♀, same data as holotype, emerged on 27-VII–2-VIII-2021.
A small wedge-shaped species (length 3.1–3.3 mm) having pronotum with posterior margin trisinuate. Elytra rather flattened, ornamentation consisting of white pubescence; on posterior half with three transverse bands, anterior one obliquely zigzag, two posterior ones transversely straight. Male genitalia with slender tegmen with paramere setiferous on anterior margin and slender pennis with rounded apex. Larvae mine leaves of Elaeocarpus japonicus.
Adult male: (Figs
Habroloma species of Japan, adult ventral views (A–J) and male genitalia of H. elaeocarpusi (K–M). A H. liukiuense B H. eximium eupoetum C H. griseonigrum D H. elaeocarpusi E H. lewisii F H. taxillusi G H. nixilla insulicola H H. yuasai I H. marginicolle J H. subbicorne K, L lateral and ventral views of tegmen, pennis and sternite IX M tergite VIII. Scale bars 1 mm (A–J); 0.5 mm (K–M).
Head , seen from above, transverse, broadly and sharply excavated between the eyes, with the inferior rim of the eyes strongly and rather suddenly produced; frons with the median impression distinct; fovea just above each antennal cavity obsolete and indistinct; surface rather smooth, sparsely scattering laterally with traces of variolate and ocellate punctures, and sparsely clothed with whitish recumbent hairs; clypeal suture transverse, somewhat arcuate exteriorly; clypeus transverse, ~ 2.6× as wide as long, with the anterior margin somewhat arcuately emarginate; antennal cavities surrounded posteriorly with elevated carina; antennae short and compact, with the third segment ~ 1.5× as long as the fourth, with apical five segments serrated.
Pronotum transverse, widest just before the base, distinctly wider than elytra, and ~ 3.2× as wide as long; sides slightly but distinctly expanded just before the base, then crescent-shaped and strongly attenuated to the anterior angles, which are acute and strongly produced in dorsal aspect; anterior margin deeply, broadly and arcuately emarginate; posterior margin trisinuate, produced and subtruncate, narrowly and slightly emarginate just before scutellum; posterior angles acute and produced posteriorly; disk dilated laterally, broadly and obsoletely depressed at the anterior half of the lateral dilation on each side, but without fovea, and obsoletely impressed along the basal lobe causing the middle of the disk to be somewhat convex; surface lustrous, punctured with traces of large, obsolete, shallow, somewhat ocellate punctures, and sparsely clothed with whitish hairs. Scutellum smooth and triangular.
Elytra rather deplanate, widest at the base, ~ 1.3× as long as wide and ~ 4.3× as long as pronotum; sides feebly sinuate and narrowed or subparallel to the anterior 2/5, and then arcuately attenuated to the apex, but the attenuation somewhat angulate near the apex; sutural margin not elevated entirely; humeri slightly prominent; basal depressions along the base transverse; lateral carinae subparallel to the lateral margin; disk constricted behind humeri, narrowly and obsoletely impressed along the inferior side of each lateral carina; surface rather uniformly but coarsely punctate with shallow, ill-defined, irregularly sized punctures, with the punctuation being somewhat rugous at the sides; ornamentation consisting of white, yellowish-grey, and blackish hairs, with the whitish hairs being predominant. Ornamentation consisting of white pubescence arranged on each elytron as follows: at base with two irregular spots, at mid length near suture with one irregular spot, toward side with one narrow, wavy, and irregular strip, on posterior half with three transverse bands, anterior one obliquely zigzag, two posterior ones transversely straight.
Body beneath scattered with very fine inconspicuous cinereous hairs. Prosternal process inverted trapezoidal, narrow toward the base, ~ 1.3× broader than long, with the apex almost truncate. Metasternum slightly convex coarsely punctate with variolate and obsolete punctures at the middle. Abdomen beneath rather uniformly punctate with shallow, obsolete variolate punctures. Legs normal; posterior coxae depressed entirely, with the latero-posterior angles acute and produced latero-posteriorly.
Male genitalia
(Fig.
Female. Like the male, but more robust. Length: 3.1–3.3 mm, width: 1.8–1.9 mm.
The name indicates the host plant genus, Elaeocarpus.
Kobanmochi-hiratachibi-tamamushi.
Elaeocarpus japonicus Sieb. et Zucc.
Primary evergreen forests dominated by Castanopsis sieboldii subsp. sieboldii.
Japan (Kyushu and Yaku Island).
Holotype : Japan: ♂ (MK-BP-k40), Yakukachi, Amami-shi, Kagoshima Pref. (28.228°N, 129.347°E, 40 m above sea level), 23-V-2009 (as larva on Taxillus yadoriki collected by A. Kawakita), emerged on 7-VI-2009, NSMT-I-C-200268.
Paratype : Japan: 1♀(MK-BP-k39), same data as holotype, emerged on 2-VI-2009, N NSMT-I-C-200269.
A small wedge-shaped species (length 2.5–2.7 mm) having pronotum with posterior margin trisinuate. Elytra slightly convex around base, ornamentation consisting of yellowish-grey pubescence; on posterior 2/3 with three transverse bands, first two obliquely zigzag, apical one slightly transversely waved. Larvae mine leaves of a mistletoe species, Taxillus yadoriki.
Adult male: (Figs
Head , seen from above, transverse, broadly excavated between the eyes, with the inferior rim of the eyes strongly produced; frons with the median impression distinct; fovea just above each antennal cavity obsolete and indistinct; surface rather smooth, sparsely scattered laterally with traces of variolate and ocellate punctures, and sparsely clothed with recumbent yellowish-grey hairs; clypeal suture transverse, somewhat arcuate exteriorly; clypeus transverse, ~ 2.6× as wide as long, with the anterior margin somewhat arcuately emarginate; antennal cavities surrounded posteriorly with elevated carina; antennae short and compact, with the third segment ~ 1.5× as long as the fourth, and five apical serrated segments.
Pronotum transverse, widest just before the base, as wide as elytra, and ~ 2.4× as wide as long; sides slightly but distinctly expanded just before the base, then crescent-shaped and strongly attenuated to the anterior angles, which are acute and strongly produced in dorsal aspect; anterior margin deeply, broadly, and arcuately emarginate; posterior margin trisinuate and subtruncate, narrowly and slightly emarginate just before scutellum; posterior angles acute and produced posteriorly; disk dilated laterally, broadly and obsoletely depressed at the anterior half of the lateral dilation on each side, but without fovea, and obsoletely impressed along the basal lobe causing the middle of the disk to be somewhat convex; surface lustrous, punctured the traces of large, obsolete, shallow, somewhat ocellate structures, and sparsely clothed with yellowish gray hairs. Scutellum smooth and triangular.
Elytra slightly convex along base, widest at the base, ~ 1.4× as long as wide and ~ 3.3× as long as pronotum; sides feebly sinuate and narrowed or subparallel to the anterior 2/5, and then arcuately attenuated to the apex but with the attenuation somewhat angulate near the apex; humeri slightly prominent; basal depressions along the base transverse; lateral carinae subparallel to the lateral margin; disk constricted behind humeri, narrowly and obsoletely impressed along the inferior side of each lateral carina; surface rather uniformly but coarsely punctate with shallow, ill-defined, irregularly sized punctures, but the punctuation somewhat rugous at the sides. Ornamentation consisting of yellowish grey pubescence arranged on each elytron as follows: at base with two irregular spots, on posterior 2/3 with three transverse bands, first and second ones obliquely zigzag, apical one slightly transversely waved; with transverse irregular spot apically.
Body beneath scattered with very fine inconspicuous cinereous hairs. Prosternal process rounded, ~ 1.27× broader than long. Metasternum slightly convex and coarsely punctate, with variolate and obsolete punctures at the middle. Abdomen beneath rather uniformly punctate with shallow, obsolete variolate punctures. Legs normal; posterior coxae depressed entirely, with the latero-posterior angles acute and produced latero-posteriorly.
Male genitalia : not studied.
Female. Like the male, but more robust. Ornamentation of elytra is similar but pubescence more whitish in female. Body length: 2.5–2.7 mm, width: 1.5–1.7 mm.
The specific name indicates host plant genus, Taxillus.
Obayadorigi-hiratachibi-tamamushi.
Taxillus yadoriki (Maxim.) Danser [Loranthaceae]
Canopy of primary evergreen forests dominated by Castanopsis sieboldii subsp. lutchuensis (Fig.
Japan (Amami-Oshima Island, known only from the type locality).
Leaf mines of Tracheini species have the following characteristics. The mined leaves are mature leaves that have completed expansion and hardening. An egg is laid on the upper side of a leaf and covered by a circular brown glossy coating, which is secreted by an adult female. Pupation takes place within the mine. Hereafter, we describe leaf mines of the 14 Habroloma and 20 Trachys species in Japan.
1. Habroloma subbicorne (Motschulsky, 1860)
Fig.
Host plant. Rosaceae: Rubus parvifolius (
Leaf mine. Brown full-depth blotch mine on mature leaf. Egg is laid at a distance from leaf margin and the mine expands in the leaf blade. Frass is thread-like.
Material examined. Nishihoragawa, Kiso, Nagano Pref. 6-VIII-2015 (as larva on Rubus palmatus var. coptophyllus), emerged on 27-VIII-2015 (Fig.
Leaf mines of Habroloma spp. on leaves of Rosaceae A–E H. subbicorne on Rubus palmatus var. coptophyllus (A), Rubus buergeri (B–D), and Rubus parvifolius (E) F H. atronitidum on Rubus vernus G–I H. marginicolle on Rubus sieboldii J H. asahinai on Rubus sieboldii K–M H. lewisii on Rosa multiflora. Arrows indicate oviposition scars.
2. H. atronitidum (Gebhardt, 1929)
Fig.
Host plant. Rosaceae: Rubus vernus (new record).
Leaf mine. Brown full-depth blotch mine on mature leaf. Egg is laid near leaf margin, and the mine expands along the leaf margin. Frass is thread-like.
Material examined. Mumyo-dani, Niimi, Okayama Pref., 9-VII-1991 (as pupa on Rubus vernus), emerged on ?-VIII-1991 (Fig.
3. H. marginicolle (Fairmaire, 1888)
Fig.
Host plant. Rosaceae: Rubus sieboldii (
Leaf mine. Brown blotch mine on mature leaf. Egg is laid at a distance from leaf margin, and the mine expands in the leaf blade. Frass is thread-like.
Material examined. Modo, Minamata, Kumamoto Pref., 26-V-2018 (vacant mine of Rubus sieboldii) (Fig.
4. H. asahinai Y. Kurosawa, 1959
Fig.
Host plant. Rosaceae: Rubus sieboldii (
Leaf mine. Brown full-depth blotch mine on mature leaf. Egg is laid near leaf margin, and the mine expands along the leaf margin. Frass is thread-like.
Material examined. Okuma, Kunigami, Okinawa Pref., 30-III-2018 (vacant mine of Rubus sieboldii) (Fig.
5. H. lewisii (E. Saunders, 1873)
Fig.
Host plant. Rosaceae: Rosa multiflora (
Leaf mine. Brown, full-depth sometimes bluish, linear-blotch mine on mature leaf. Egg is laid along leaf margin, and the mine expands along leaf margin. Frass is thread-like, coiling or undulating for an extended length.
Material examined. Sabushi-gawa, Niimi, Okayama Pref., 1-VII-2018 (as larva on Rosa multiflora), emerged on 18-VII-2018 (Fig.
6. H. griseonigrum (E. Saunders, 1873)
Fig.
Host plant. Fagaceae: Quercus glauca, Q. acutissima, Q. serrata (
Leaf mines of Habroloma spp. on leaves of Fagaceae, Juglandaceae, Elaeocarpaceae, Lythraceae, and Loranthaceae A H. griseonigrum on Quercus hondae B–D H. yuasai on Platycarya strobilacea E–G H. elaeocarpusi on Elaeocarpus japonicus H, I H. nixilla on Lagerstroemia subcostata J–L H. taxillusi on Taxillus yadoriki. Arrows indicate oviposition scars.
Leaf mine. Brown full-depth blotch mine on mature leaf. Egg is laid near leaf margin of leaf base, and the mine expands upwards along leaf margin. Frass is thread-like.
Material examined. Gion, Yayoi, Saeki, Ooita Pref., 14-VI-1998 (as larva on Quercus hondae); emerged on 8-VII-1998 (Fig.
7. H. yuasai Y. Kurosawa, 1976
Fig.
Host plant. Juglandaceae: Platycarya strobilacea (
Leaf mine. Brown full-depth linear-blotch mine on mature leaf. Egg is laid near leaf margin, and the mine expands along leaf margin. Frass is thread-like, excreted from the mine through cracks of upper epidermis.
Material examined. Makido, Niimi, Okayama Pref., 22-VI-2020 (as larva on Platycarya strobilacea), emerged on 20-VII-2020 (Fig.
8. H. elaeocarpusi sp. nov
Fig.
Host plant. Elaeocarpaceae: Elaeocarpus japonicus (new record).
Leaf mine. Pale brown full-depth linear-blotch mine on mature leaf. Egg is laid just beside midrib of leaf base, and the mine expands along midrib or along leaf margin. Frass is thread-like; frass line iterating arc-shaped reciprocating motion.
Material examined. Mt. Osuzu, Tsuno-cho, Miyazaki Pref., 14-VII-2021 (as larva on Elaeocarepus japonicus), emerged on 27-VII-2021 (Fig.
9. H. bifrons (Kiesenwetter, 1879)
Host plant. Unknown, while related species in Europe is associated with Geranium (Geraniaceae).
Leaf mine. Unknown.
10. H. nixilla insulicola Y. Kurosawa, 1959
Fig.
Host plant. Lythraceae: Lagerstroemia subcostata (
Leaf mine. Brown full-depth linear-blotch mine on mature leaf. Egg is laid along leaf margin of leaf base, and the mine expands along leaf margin. Frass is thread-like, coiling in the mine.
Material examined. Sumiyo, Amami, Kagoshima Pref., 23-V-2009 (as larva on Lagerstroemia subcostata), emerged on 6-VI-2009 (Fig.
11. H. taxillusi sp. nov.
Fig.
Host plant. Loranthaceae: Taxillus yadoriki (new record).
Leaf mine. Brown full-depth linear-blotch mine on mature leaf. Egg is laid along leaf margin of leaf base, and the mine expands along leaf margin. Frass is granular, accumulated in the center of the mine.
Material examined. Yakukachi, Amami-shi, Kagoshima Pref., 23-V-2009 (as larva on Taxillus yadoriki), emerged on 7-VI-2009 (Fig.
12a. H. eximium eximium (Lewis, 1893)
Host plant. Symplocaceae: Symplocos lancifolia (
Leaf mine. Unknown.
12b. H. eximium eupoetum (Obenberger, 1929)
Fig.
Host plant. Symplocaceae: Symplocos prunifolia (
Leaf mine. Pale brown full-depth linear-blotch mine on mature leaf. Egg is laid near midrib of leaf base, and the hatched larva enters midrib and bores into petiole, causing the leaf to fall off from the branch by being abscised at the petiole base. After the leaf-fall, the mine departs from the midrib and slowly expands upwards along leaf margin or along midrib. After advancing halfway, the mine abruptly expands to become a blotch mine. Frass is thread-like, going in a zigzag in the early linear mine, and becomes thick cord-like without undulating as the mine expands. The fallen leaf is kept green for ca. two weeks, during which the larva completes its development.
Material examined. Komi, Iriomote Is., Yaeyama, Okinawa Pref., 10-V-2020 (as larva on Symplocos caudata), emerged on 15-VI-2020 (Fig.
13. H. liukiuense (Obenberger, 1940)
Fig.
Host plant. Symplocaceae: Symplocos okinawensis (
Leaf mine. Pale brown full-depth linear-blotch mine on mature leaf. Egg is laid near midrib of leaf base, and the hatched larva enters midrib and bores into petiole, causing the leaf to fall off from the branch by being abscised at the petiole base. After the leaf-fall, the mine departs from the midrib and slowly expands upwards along leaf margin or along midrib. After advancing halfway, the mine abruptly expands to become a blotch mine. Frass is thread-like, going in a zigzag in the early linear mine, and becomes granular. The fallen leaf is kept green for ca. two weeks, during which the larva completes its development.
Material examined. Foothill of Mt. Yonaha, Kunigami, Okinawa Pref., 6-VI-2018 (as larva on Symplocos microcalyx), emerged on 12-VII-2018 (Fig.
14. H. hikosanense Y. Kurosawa, 1959
Host plant. Unknown.
Leaf mine. Unknown.
1. Trachys auricollis E. Saunders, 1873
Fig.
Host plant. Fabaceae: Pueraria montana var. lobata (
Leaf mine. Gray full-depth blotch mine on mature leaflet. Egg is laid in an inner area of leaf blade, and mine expands toward leaf margin. Frass is granular and distributed all over the mine.
Material examined. Mt. Osuzu, Tsuno, Miyazaki Pref., 14-VII-2021 (as larva), emerged on 30-VII-2021 (Fig.
2. Trachys reitteri Obenberger, 1930
Fig.
Host plant. Fabaceae: Amphicarpaea edgeworthii (
Leaf mine. White full-depth blotch mine on mature leaflet. Egg is laid along anterior margin of a leaflet, and the mine expands toward leaf base. Frass is granular and distributed all over the mine.
Materials examined. Kiso-Fukushima, Nagano Pref., 10-VIII-2019 (as larva on A. edgeworthii), emerged on 23-VII-2019 (Fig.
Leaf-mines of Trachys spp. on leaves of Fabaceae and Rosaceae A T. auricollis on Pueraria montana var. lobata B–D T. reitteri on Amphicarpaea edgeworthii (B, C) and Glycine soja (D) E T. tokyoensis on Desmodium podocarpum subsp. fallax F T. toringoi on Chaenomeles japonica G T. inconspicuus on Prunus mume. Arrows indicate oviposition scars.
3. Trachys tokyoensis Obenberger, 1940
Fig.
Host plant. Fabaceae: Desmodium podocarpum subsp. oxyphyllum (
Leaf mine. Brown full-depth blotch mine on mature leaflet. Egg is laid along anterior margin of a leaflet, and the mine expands toward leaf base. Frass is granular and distributed all over the mine.
Material examined. Kawaguchi-ko Lake, Yamanashi Pref., 19-IX-2017 (as pupa on Desmodium podocarpum subsp. fallax), emerged on 10-X-2017 (Fig.
4. Trachys toringoi Y. Kurosawa, 1951
Fig.
Host plant. Rosaceae: Chaenomeles japonica, Malus sieboldii (
Leaf mine. Brown blotch mine on mature leaf. Egg is laid in an inner basal area of leaf blade, and the mine expands toward leaf top. Frass is granular and distributed all over the mine.
Material examined. Hara-mura, Suwa, Nagano Pref., 15-VIII-2018 (as vacant mine of Chaenomeles japonica) (Fig.
5. Trachys inconspicuus E. Saunders, 1873
Fig.
Host plant. Rosaceae: Prunus mume, P. salicina (
Leaf mine. White full-depth blotch mine on mature leaf. Egg is laid along leaf margin, and the mine expands along leaf margin. Frass is granular and distributed all over the mine.
Material examined. Kamikoma, Yamashiro, Kizugawa, Kyoto Pref., 17-VI-2016 (as pupa on Prunus mume), emerged on 19-VI-2016 (Fig.
6. Trachys pecirkai Obenberger, 1925
Fig.
Host plant. Ulmaceae: Ulmus davidiana var. japonica (
Leaf mine. Brown full-depth blotch mine on mature leaf. Egg is laid near midrib, and the mine expands along lateral vein and then along leaf margin. Frass is granular and distributed all over the mine.
Material examined. Suekawa, Kaida, Kiso, Nagano Pref., 5-VIII-2022 (as vacant mine of Ulmus davidiana var. japonica) (Fig.
Leaf mines of Trachys spp. on leaves of Ulmaceae and Cannabaceae A T. pecirkai on Ulmus davidiana var. japonica B–D T. yanoi on Zelkova serrata E, F T. cupricolor on Zelkova serrata G, H T. griseofasciatus on Aphananthe aspera I T. ineditus on A. aspera J–L T. broussonetiae on Broussonetia kazinoki (J) and Fatoua villosa (K, L). Arrows indicate oviposition scars.
7. Trachys cupricolor E. Saunders, 1873
Fig.
Host plant. Ulmaceae: Zelkova serrata (
Leaf mine. Dark brown full-depth blotch mine on mature leaf. Egg is laid along midrib, and the mine expands along leaf margin. Frass is granular and distributed all over the mine.
Material examined. Mt. Ibuki, Maibara, Shiga Pref., 29-VII-2020 (as pupa on Zelkova serrata), emerged on 25-VIII-2020 (Fig.
8. Trachys yanoi Y. Kurosawa, 1959
Fig.
Host plant. Ulmaceae: Zelkova serrata (
Leaf mine. Brown full-depth blotch mine on mature leaf. Egg is laid along leaf margin, and the mine expands along leaf margin. Frass is granular and distributed all over the mine.
Material examined. Mt. Shizuhata, Aoi-ku, Shizuoka, Shizuoka Pref., 27-VI-2018 (as pupa on Zelkova serrata), emerged on 12-VII-2018 (Fig.
9. Trachys griseofasciatus E. Saunders, 1873
Fig.
Host plant. Cannabaceae: Aphananthe aspera (
Leaf mine. Dark brown full-depth blotch mine on mature leaf. Egg is laid along anterior leaf margin, and the mine expands along leaf margin. Frass is thread-like and distributed all over the mine.
Material examined. Demachi-yanagi, Shimogamo, Sakyo, Kyoto Pref., 1-VII-2020 (as pupa on Aphananthe aspera), emerged on 7-VIII-2020 (Fig.
10. Trachys ineditus E. Saunders, 1873
Fig.
Host plant. Cannabaceae: Aphananthe aspera (
Leaf mine. White full-depth blotch mine on mature leaf. Egg is laid along leaf margin, and the mine expands along leaf margin. Frass is granular and distributed all over the mine.
Material examined. Mukoujima, Uji, Kyoto Pref., 15-VII-2012 (as larva on Aphananthe aspera), emerged on 28-VII-2012 (Fig.
11. Trachys broussonetiae Y. Kurosawa, 1985
Fig.
Host plant. Moraceae: Broussonetia kazinoki (
Leaf mine. White full-depth blotch mine on mature leaf. Egg is laid along leaf margin, and the mine expands along leaf margin. Frass is granular and distributed all over the mine.
Materials examined. Chigonosawa, Kiso-fukushima, Kiso, Nagano Pref., 4-VIII-2018 (as larva on Broussonetia kazinoki), emerged on 30-VIII-2018 (Fig.
12. Trachys variolaris E. Saunders, 1873
Fig.
Host plant. Fagaceae: Quercus glauca, Q. serrata, Q. variabilis (
Leaf mine. Gray full-depth blotch mine on mature leaf. Egg is laid near leaf margin, and the mine expands along leaf margin. Frass is granular and distributed all over the mine.
Material examined. Inohae-keikoku, Kitago, Nichinan, Miyazaki Pref., 16-VI-2019 (as larva on Quercus hondae), emerged on 19-VII-2019 (Fig.
13. Trachys dilaticeps Gebhardt, 1929
Fig.
Host plant. Fagaceae: Castanopsis sieboldii subsp. lutchuensis (
Leaf mine. Gray full-depth linear-blotch mine on mature leaf. Egg is laid along midrib near leaf tip, and the mine expands downwards along leaf margin. Frass is granular and distributed all over the mine.
Material examined. Mt. Nishime, Kunigami, Okinawa Pref., 6-VI-2018 (as larva on Castanopsis sieboldii subsp. lutchuensis), emerged on 6-VII-2018 (Fig.
14. Trachys robustus E. Saunders, 1873
Fig.
Host plant. Fagaceae: Castanopsis sieboldii subsp. sieboldii (
Leaf mine. Gray full-depth linear-blotch mine on mature leaf. Egg is laid along midrib near leaf tip, and the mine expands downwards along leaf margin. Frass is granular and distributed all over the mine.
Material examined. Mt. Shizuhata, Aoi-ku, Shizuoka, Shizuoka Pref., 27-VI-2018 (as larva on Castanopsis sieboldii subsp. sieboldii), emerged on 13-VII-2018 (Fig.
15. Trachys minutus salicis (Lewis, 1893)
Fig.
Host plant. Salicaceae: Salix caprea, S. miyabeana subsp. gymnolepis, S. vulpina, Populus maximowiczii (
Leaf mine. Brown full-depth blotch mine on mature leaf. Egg is laid just near leaf tip, and the mine expands downwards along leaf margin. Frass is granular and connected.
Material examined. Matsuo-kozan, Hachimantai, Iwate Pref., 7-VII-2018 (as larva on Salix reinii), emerged on 30-VII-2018 (Fig.
Leaf mines of Trachys spp. on leaves of Salicaceae, Violaceae, Malvaceae, and Hydrangeaceae. A–C T. minutus on Salix reinii (A, B) and Salix vulpina (C) D–F T. pseudoscrobiculatus on Viola grypoceras G T. aurifluus on Tilia maximowicziana H, I T. saundersi on Deutzia crenata (H) and Deutzia gracilis (I) J–L T. tsusimae on Deutzia scabra. Arrows indicate oviposition scars.
16. Trachys pseudoscrobiculatus Obenberger, 1940
Fig.
Host plant. Violaceae: Viola grypoceras.
Leaf mine. White full-depth linear-blotch mine on mature leaf. Egg is laid along leaf margin near leaf tip, and the mine expands downwards along leaf margin. Frass is granular and connected.
Material examined. Mt. Mikusa, Kato-shi, Hyogo Pref., 20-V-2018 (as larva on Viola grypoceras), emerged on 8-VI-2018 (Fig.
17. Trachys aurifluus Solsky, 1875
Fig.
Host plant. Malvaceae: Tilia maximowicziana, T. japonica (
Leaf mine. Brown full-depth linear-blotch mine on mature leaf. Egg is laid near leaf margin, and the mine expands along leaf margin. Frass is granular and distributed all over the mine.
Material examined. Iyari, Inao, Omachi, Nagano Pref., 1-VII-2013 (vacant mine of Tilia maximowicziana) (Fig.
18. Trachys saundersi Lewis, 1893
Fig.
Host plant. Hydrangeaceae: Deutzia crenata (
Leaf mine. Brown full-depth blotch mine of whole layers of leaf blade. Egg is laid along leaf margin near leaf base, and the mine expands upwards along leaf margin. Frass is granular and loosely connected.
Material examined. Makido, Niimi, Okayama Pref., 1-VII-2018 (as larva on Deutzia crenata), emerged on 17-VII-2018 (Fig.
19. Trachys tsusimae Obenberger, 1922
Fig.
Host plant. Hydrangeaceae: Deutzia crenata (
Leaf mine. Brown full-depth blotch mine on mature leaf. Egg is laid along leaf margin, and the mine expands along leaf margin. Frass is granular and distributed all over the mine.
Material examined. Mt. Osuzu, Tsuno, Miyazaki Pref., 14-VII-2021 (as larva on Deutzia scabra), emerged on 27-VII-2021 (Fig.
20. Trachys cuneiferus Y. Kurosawa, 1959
Host plant. Unknown.
Leaf mine. Unknown.
After adding the two new species, we counted 34 trachyine species in the Japanese Archipelago (Table
A list of Japanese species of the tribe Trachyini, with their host plant species.
Buprestids | Host plants | |||||
---|---|---|---|---|---|---|
Genus | Species | Subclass | Order | Family | Genera | Habit |
Habroloma | subbicorne | Rosids | Rosales | Rosaceae | Rubus | evergreen/ deciduous shrub |
atronitidum | Rosaceae | Rubus | deciduous shrub | |||
marginicolle | Rosaceae | Rubus | evergreen shrub | |||
asahinai | Rosaceae | Rubus | evergreen shrub | |||
lewisii | Rosaceae | Rosa | deciduous shrub | |||
griseonigrum | Fagales | Fagaceae | Quercus | evergreen/ deciduous tree | ||
yuasai | Juglandaceae | Platycarya | deciduous tree | |||
elaeocarpusi n. sp | Oxalidales* | Elaeocarpaceae* | Elaeocarpus* | evergreen tree | ||
bifrons | Geraniales? | Geraniaceae ? | Geranium? | perennial ? | ||
nixilla insuicola | Myrtales | Lythraceae | Lagerstroemia | deciduous tree | ||
taxillusi n. sp | Santalales* | Loranthaceae* | Taxillus* | evergreen epiphyte | ||
eximium eximium | Asterids | Ericales | Symplocaceae | Symplocos | evergreen tree | |
eximium eupoetum | Symplocaceae | Symplocos | evergreen tree | |||
liukiuense | Symplocaceae | Symplocos | evergreen shrub | |||
hikosanense | ? | ? | ? | |||
Trachys | auricollis | Rosids | Fabales | Fabaceae | Pueraria | deciduous liana |
reitteri | Fabaceae | Amphicarpaea, Glycine, Pueralia, Rhynchosia | deciduous liana | |||
tokyoensis | Fabaceae | Desmodium | perennial | |||
toringoi | Rosales | Rosaceae | Amelanchier, Chaenomeles, Cydonia, Malus, Pyrus | deciduous tree | ||
inconspicuus | Rosaceae | Prunus | deciduous tree | |||
pecirkai | Ulmaceae | Ulmus | deciduous tree | |||
cupricolor | Ulmaceae | Zelkova | deciduous tree | |||
yanoi | Ulmaceae | Zelkova | deciduous tree | |||
griseofasciatus | Cannabaceae | Aphananthe, Celtis | deciduous tree | |||
ineditus | Cannabaceae | Aphananthe | deciduous tree | |||
broussonetiae | Moraceae | Broussonetia, Fatoua* | deciduous tree/ annual | |||
variolaris | Fagales | Fagaceae | Quercus | evergreen tree | ||
dilaticeps | Fagaceae | Castanopsis | evergreen tree | |||
robustus | Fagaceae | Castanopsis | evergreen tree | |||
minutus salicis | Malpighiales | Salicaceae | Salix, Poplus | deciduous tree | ||
pseudoscrobiculatus | Violaceae | Viola | perennial | |||
aurifluus | Malvales | Malvaceae | Tilia | deciduous tree | ||
saundersi | Asterids | Cornales | Hydrangeaceae | Deutzia | deciduous shrub | |
tsusimae | Hydrangeaceae | Deutzia | deciduous shrub | |||
cuneiferus | ? | ? | ? | ? |
The host plant genera of the two new trachyine species are Elaeocarpus and Taxillus, belonging to Elaeocarpaceae (Oxalidales) and Loranthaceae (Santalales), respectively, and representing the first records in Tracheini for both families and orders, while both plant families have been recorded as hosts for Agrilus (Jendek & Poláková, 2014). The record on Taxillus is also the first record of buprestids associated with epiphytic, plant-parasitic plants.
Our records of leaf mines suggest that those of trachyine species are generally full-depth blotch mines on mature leaves of woody or subwoody plants, except for one species (Trachys pseudoscrobiculatus) associated with Viola. These leaf mines contrast with upper-layer mines on young leaves formed by agromyzids, epidermal/mesophyll mines on young leaves formed by gracillariids, thin full-depth linear mines formed by Lyonetiidae, and full-depth linear-blotch mines on young leaves formed by Eriocraniidae.
One of the authors (MK) is indebted to the late Prof. Yoshihiko Kurosawa for helping to obtain references on Buprestidae in 1990s. This work was supported by a Japan Ministry of Education, Culture, Science, Sports, and Technology Grant-in-Aid for Scientific Research (#15H02420, #20H03321).