Research Article |
Corresponding author: Scott A. Schneider ( scott.schneider@ars.usda.gov ) Academic editor: Takumasa Kondo
© 2022 Scott A. Schneider, John S. LaPolla.
This is an open access article distributed under the terms of the CC0 Public Domain Dedication.
Citation:
Schneider SA, LaPolla JS (2022) A Neotropical complex of Ripersiella species (Hemiptera, Coccomorpha, Rhizoecidae) collected from the nests of Acropyga ants (Hymenoptera, Formicidae). ZooKeys 1123: 1-30. https://doi.org/10.3897/zookeys.1123.90141
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We describe five new Neotropical species of Ripersiella living in association with Acropyga ants: R. campensis sp. nov., R. illicians sp. nov., R. montanae sp. nov., R. pediandensis sp. nov., and R. telalia sp. nov. We also redescribe R. andensis and R. colombiensis based on type specimens and other collections. Together, these seven species form a morphologically similar group that we informally refer to as the andensis-complex of Ripersiella. All members of the andensis-complex are confirmed or are speculated to be mutualists of Acropyga ants. We discuss the implications of these associations and provide an identification key to the Neotropical species of Ripersiella that are lacking bitubular cerores, including the new species.
Coccoidea, mutualism, root mealybug, taxonomy, trophobiosis
Acropyga Roger ants (Hymenoptera, Formicidae) are obligatory mutualists of scale insects. They primarily associate with root mealybugs from the family Xenococcidae Tang (Hemiptera, Coccomorpha), who are likewise obligate mutualists of Acropyga (
Some species of root mealybugs from the genus Ripersiella Tinsley (Hemiptera, Rhizoecidae) have previously been reported as mutualists of Acropyga ants, including R. andensis (Hambleton) (
In this article, we describe five new species of Ripersiella from Peru and the Dominican Republic. In combination with R. andensis and R. colombiensis, these seven species form a complex that we refer to informally here as the andensis-complex. Furthermore, we provide a key to the Neotropical species of Ripersiella which lack bitubular cerores to aid in the identification of species in the andensis-complex. A thorough treatment of the Ripersiella species from the Neotropical region was recently provided by
Many reports on the trophobiosis between Acropyga ants and scale lineages falling outside of Xenococcidae require verification to confirm that the ant and scale species were in fact directly associated (see
Specimens were preserved in 95–100% ethanol and stored at −80 °C prior to preparation. Examined specimens were prepared either by slide mounting directly or by first extracting DNA prior to mounting their cuticle. Extractions were performed using the QIAamp DNA Mini Kit (Qiagen, Valencia, California) following the standard protocol; specimen cuticles were removed from the extraction buffer after the initial lysing step and subsequently mounted. DNA-extracted specimens were assigned a unique six-digit alphanumeric identifier beginning with “S” followed by four numbers used to identify the collection event and ending with a sequentially assigned letter to identify the individual specimen (e.g., S0439A). Their DNA extractions, preserved at −80 °C, are housed at the USDA ARS Beltsville Agricultural Research Center, Beltsville, Maryland, USA. Individuals that were slide-mounted directly are identified with a sequentially assigned letter appended to the end of the collector number (e.g., JSL090804-05A). All specimens were slide-mounted following the protocol described in
The terminology used in this paper follows
Identification of associated Acropyga ants was performed using the key to species provided by
Type depositories are abbreviated as follows:
For our collections from Peru, we confirmed direct trophobiotic association between root mealybugs and Acropyga ants through careful observation of interacting partners using a nest-box, following the protocol described by
Ripersiella Tinsley in Cockerell, 1899: 278. Type species: Ripersia rumicis Maskell, 1892.
Rhizoecus (Pararhizoecus) Goux, 1941: 197. Type species: Rhizoecus petiti Goux, 1941.
Pararhizoecus
Goux, 1941;
The new species described below are placed in Ripersiella based on the following diagnosis, which is a condensed version of the comprehensive descriptions provided by
Tritubular cerores (also referred to as tritubular pores or ducts) absent; bitubular cerores (bitubular pores/ducts) typically present, absent in some species; anal ring with or without elongate cells, lacking protuberances, and situated dorsally; body setae all flagellate, anal lobes usually poorly developed, bearing a set of 3 distinct long setae or with several short setae; trilocular pores present but never arranged in tight clusters on the venter; body oval to spherical and membranous; antennae geniculate with 5 or 6 segments.
In
Neorhizoecus andensis Hambleton, 1946: 41.
Rhizoecus andensis
(Hambleton);
?Ripersiella andensis (Hambleton);
Lectotype. Colombia • 1 adult ♀; Bogota; 22.ii.1935; L.M. Murillo; on roots of Coffea arabica L.;
Adult female (N = 6). Appearance in life not recorded, extent of wax production unknown.
General. Mounted on microscope slide, body broadly oval and membranous, 0.78–1.06 (0.98) mm long, widest near abdominal segments II–III, 0.41–0.58 (0.52) mm wide. Abdomen smoothly tapering toward apex; abdominal segment VIII 170–213 (180) μm wide at base. Anal lobes poorly developed with several flagellate setae on venter and dorsum, ranging from 15–33 μm long. Body setae flagellate, 10–22 μm on head, 9–15 μm on thorax, 11–17 μm on abdominal segments. Trilocular pores abundant and distributed among body setae; bitubular cerores absent; oral collar tubular ducts absent. Microtrichia present on abdominal segments. Eyes absent.
Venter. Cephalic plate absent. Labium with 3 segments; 70 μm long and 46 μm at widest point. Antennae geniculate, 5-segmented, closely situated near midline on ventral submargin of head; overall length 126–134 (132) μm; length of segment I: 27–38 (34) μm; segment II: 14–17 (16) μm; segment III: 14–16 (15) μm; segment IV: 13–18 (14) μm; segment V: 52–53 (53) μm; apical antennal segment with 1 spine-like and 4 falcate stout sensory setae; few flagellate setae on each antennal segment, 14–35 μm long; sensorium present on second antennal segment. Legs well developed; overall length of hind leg 235–241 (238) μm; length of hind coxa 28–34 (34) μm; hind trochanter + femur 91–95 (91) μm; hind tibia + tarsus 88–91 (91) μm; hind claw 22–25 (22) μm; each claw with short setose digitule 2–3 μm long; flagellate setae present on each segment, around 19 μm long. Circulus absent. Multilocular disc pores in irregular rows or groups on segments VI–VIII, pores with 6–8 loculi in the outer ring.
Dorsum. Anal ring on dorsal surface near posterior margin, 48–52 μm in diameter; with oval cells, some cells bearing spicules; with three pairs of setae 33–39 μm long. Posterior pair of dorsal ostioles present, diameter of orifice measured along longitudinal axis approximately 45 μm; anterior pair present but much smaller than posterior pair and poorly developed, diameter of orifice approximately 10 μm. Multilocular disc pores absent.
The following newly described species are similar in appearance to R. andensis: R. campensis, R. montanae, and R. pediandensis. Consult the respective diagnosis sections under each species below for a discussion on how to distinguish them from R. andensis. Another new species, R. telalia, can easily be distinguished by the presence of well-developed anterior ostioles, which are present in R. andensis but are strongly reduced and may appear to be absent.
Ripersiella andensis may associate with Acropyga exsanguis and A. smithii (
Holotype. Dominican Republic • 1 adult ♀; Loma Novillero (Fonestal Reserva) near Villa Altagracia; 18.7032, -70.1931, elev. 187 m; 4.viii.2009; JS LaPolla, SA Schneider leg.; associated with Acropyga dubitata, nest in 2° forest at base of tree root;
Adult female (N = 17). In life, body bright white to cream colored and free of obvious waxy secretions, small deposits of wax from trilocular pores visible under SEM (Fig.
Ripersiella campensis Schneider & LaPolla sp. nov. Scanning electron micrographs (SEM) by J. Mowery (USDA ARS SEL) A dorsal view B ventral ½ view of adult female C magnified view of miniscule anterior dorsal ostiole D magnified view of curled wax deposits, trilocular pores, spine-like microtrichia, and abundant domed dermal micro-bumps in intersegmental region.
General. Mounted on microscope slide, body broadly oval and membranous, 0.86–1.03 (0.93) mm long, widest near abdominal segments III–V, 0.50–0.70 (0.60) mm wide. Abdomen slightly constricted between segments VII and VIII or smoothly tapering; abdominal segment VIII 186–251 (210) μm wide at base. Anal lobes poorly developed with several flagellate setae on venter and dorsum, ranging from 23–75 μm long, with 1 or 2 longer setae situated near dorsal margin. Body setae flagellate, 14–40 μm on head, 13–33 μm on thorax, 14–37 μm on abdominal segments. Trilocular pores abundant and distributed among body setae; bitubular cerores absent; oral collar tubular ducts absent. Microtrichia present on abdominal segments; rounded dermal micro-bumps abundant in intersegmental areas and surroundings of appendages. Eyes absent.
Venter. Cephalic plate absent. Labium with 3 segments; 71 μm long and 43 μm at widest point. Antennae geniculate, 5-segmented, closely situated near midline on ventral submargin of head; overall length 140–156 (146) μm; length of segment I: 33–43 (34) μm; segment II: 17–21 (17) μm; segment III: 14–19 (16) μm; segment IV: 14–19 (18) μm; segment V: 53–60 (58) μm; apical antennal segment with 1 spine-like seta, 4 falcate stout sensory setae, and what appears to be 1 minute sensory seta at terminal apex; few flagellate setae on each antennal segment, 25–35 μm long; sensorium present on second antennal segment. Legs well developed; overall length of hind leg 266–292 (280) μm; length of hind coxa 34–49 (34) μm; hind trochanter + femur 101–115 (103) μm; hind tibia + tarsus 103–108 (105) μm; hind claw 23–27 (26) μm; each claw with short setose digitule 2–3 μm long; flagellate setae present on each segment, around 27 μm long. Circulus absent. Multilocular disc pores in irregular rows or groups on segments III–VIII, sometimes missing from segments III or IV but always present at least as far anterior as V, pores with 7 loculi in the outer ring.
Dorsum. Anal ring on dorsal surface separated from posterior body margin by approximately 1× diameter of ring, 49–59 μm in diameter; with oval cells, some cells bearing spicules; with 3 pairs of setae 40–54 μm long. Posterior pair of dorsal ostioles present, diameter of orifice measured along longitudinal axis approximately 45 μm; anterior pair present but much smaller than posterior pair and poorly developed, diameter of orifice approximately 10 μm. Multilocular disc pores absent.
Specimens of R. campensis have been previously referred to in the literature as “Rhizoecus new sp.” (
The species epithet is an adjective formed from the Latin noun campus referring to “a level place or surface” with the suffix -ensis denoting “of or from a place” alluding to the type series being collected only from lowland areas of Hispaniola.
Ripersiella campensis sp. nov. is similar in appearance to R. montanae sp. nov., described below. Morphological differences between the two species are subtle, but they can be distinguished based on the following suite of characteristics. In R. campensis, multilocular disc pores are present on abdominal segments V–VIII and usually present on segments III–IV as well, body setae are comparatively longer and sparsely distributed, antennal segments II–IV are subequal in length (average lengths in μm: 19, 16, 17), and segment V is approximately 57 μm long. In R. montanae, multilocular disc pores are restricted to abdominal segments VI–VIII, body setae are comparatively shorter and densely distributed, antennal segments II–IV differ in length (average lengths in μm: 13, 24, 20), and segment V is approximately 40 μm long.
Ripersiella campensis is also similar in appearance to R. andensis. The two species can be distinguished as follows (character states for R. andensis are given in parentheses): having multilocular disc pores on any of segments III–V (absent on these segments); having anal lobe setae as long as 75 μm (as long as 35 μm); setae on the head, thorax, and abdomen are exceeding 30 μm (not exceeding 25 μm); hind legs are approximately 280 μm long (240 μm); and antennae are approximately 146 μm long (128 μm).
Ripersiella campensis was discovered from five nests of Acropyga dubitata (Wheeler & Mann) (nests DR3–6,8). The nests were located in lowland (between 112–290 m) forested areas, including a mixed forest/cacao plantation, riparian forest, and secondary growth forest near agricultural fields. We verified direct species-to-species association (trophobiosis) between the scale insects and ants through observation of attendance by worker ants and by the fact that all colonies contained numerous individuals of the same root mealybug species within their nest chambers and no additional species of scale insects were present. In the Dominican Republic, R. montanae also associates with A. dubitata but potentially only in areas of high elevation (>1000 m) in the mountains near the shared border with Haiti.
Neorhizoecus colombiensis Hambleton, 1946: 43.
Rhizoecus colombiensis
(Hambleton);
Ripersiella colombiensis
(Hambleton);
Holotype. Colombia • 1 adult ♀; La Esperanza; ii.1936; R Roba coll.;
Adult female, based on holotype. Appearance in life not reported; extent of wax production unknown.
General. Mounted on microscope slide, body broadly oval and membranous, 1.09 mm long, widest at metathorax and abdominal segments I–II, 0.91 mm wide. Abdomen rounded and gently tapering toward posterior apex; abdominal segment VIII approximately 270 μm wide at base. Anal lobes poorly developed with several flagellate setae on dorsum and venter, 18–28 μm long; lacking differentiated group of 3 long anal lobe setae. Body setae flagellate, 17–25 μm on head, 15–20 μm on thorax, 16–21 μm on abdominal segments. Trilocular pores scarcely distributed among body setae; multilocular disc pores absent; bitubular cerores absent; oral collar tubular ducts absent. Microtrichia present on abdominal segments and thorax. Eyes absent.
Venter. Cephalic plate absent. Labium with 3 segments; 88–110 μm long and 69 μm at widest point. Antennae geniculate, 5-segmented, closely situated near midline on ventral submargin of head; overall length 182 μm; length of segment I: 36 μm; segment II: 26 μm; segment III: 28 μm; segment IV: 22 μm; segment V: 70 μm; apical segment with 1 spine-like and 4 falcate sensory setate; flagellate setae on each antennal segment 18–40 μm long. Legs well developed; overall length of hind leg 394 μm; length of hind coxa 48 μm; length of hind trochanter + femur 158 μm; length of hind tibia + tarsus 152 μm; length of hind claw 36 μm; each claw with setose digitule 4 μm long; flagellate or stout setae present on each segment 18–28 μm long. Single conical circulus present between abdominal segments III and IV, 28 μm wide; inner margin of orifice slightly crenulated.
Dorsum. Anal ring situated on dorsal surface separated from posterior body margin by approximately 1× diameter of ring, 80 μm in diameter; with oval cells lacking spicules; bearing three pairs of setae about 63 μm long. Posterior pair of ostioles present, diameter of orifice measured along longitudinal axis approximately 68 μm; anterior pair of ostioles apparently absent.
Ripersiella colombiensis is most similar in appearance to the newly described species R. illicians. Consult the diagnosis of R. illicians for a discussion on how to distinguish them.
Ripersiella colombiensis is confirmed to associate with Acropyga epedana and is one of few species of Rhizoecidae with a published record of Acropyga queens carrying gravid female trophobionts on their nuptial flight (
Holotype. Peru • 1 adult ♀; Madre de Dios, Manu National Park, Cocha Cashu Biological Station, near trail marker 27:1150; -11.8833, -71.4000; 10.vi.2019; JS LaPolla, SA Schneider leg.; upland forest, from large nest of Acropyga goeldii (group) at base of tree;
Adult female (N = 7). In life, body bright white to cream colored and visibly coated in powdery white wax.
Ripersiella illicians Schneider & LaPolla sp. nov. Scanning electron micrograph (SEM) by J. Mowery (USDA ARS SEL). Dorsal anterior view of adult female head and thorax, showing the absence of anterior ostioles and the presence of curled wax deposits and domed dermal micro-bumps in intersegmental areas.
General. Mounted on microscope slide, body broadly oval and membranous, 0.70–0.83 (0.78) mm long, widest at metathorax and abdominal segments I–II, 0.46–0.61 (0.55) mm wide. Abdomen rounded and gently tapering toward posterior apex; abdominal segment VIII 148–196 (196) μm wide at base. Anal lobes poorly developed with several flagellate setae on dorsum, 57–60 μm long. Body setae flagellate, 15–27 μm on head, 17–33 μm on thorax, 15–40 μm on abdominal segments. Trilocular pores abundant and distributed among body setae; multilocular disc pores absent; bitubular cerores absent; oral collar tubular ducts absent. Microtrichia apparently present only on dorsum of segments VI–VIII; rounded dermal micro-bumps present in intersegmental areas and surroundings of appendages. Internal genital organ sclerotized in some specimens of type series. Eyes absent.
Venter. Cephalic plate present. Labium with 3 segments; 82 μm long and 41 μm at widest point. Antennae geniculate, 6-segmented, closely situated near midline on ventral submargin of head; overall length 130–151 (151) μm; length of segment I: 30–37 (37) μm; segment II: 14–18 (18) μm; segment III: 17–20 (20) μm; segment IV: 14–19 (19) μm; segment V: 13–17 (17) μm; segment VI: 39–40 (40) μm; apical segment with 4 falcate stout sensory setate; a few flagellate setae present on each antennal segment, 19–30 μm long. Legs well developed; overall length of hind leg 274–290 (288) μm; length of hind coxa 43–44 (44) μm; length of hind trochanter + femur 102–108 (106) μm; length of hind tibia + tarsus 102–110 (110) μm; length of hind claw 26–31 (28) μm; each claw with stout setose digitule 1–2 μm long; flagellate setae present on each segment approximately 23 μm long. Single conical circulus present between abdominal segments III and IV, 22 μm in diameter; inner margin of orifice crenulated or with rugose projections.
Dorsum. Anal ring situated on dorsal surface separated from posterior body margin by approximately one-half diameter of ring, 59–61 μm in diameter; with oval cells lacking spicules; bearing 3 pairs of setae about 50 μm long. Posterior pair of ostioles present, diameter of orifice measured along longitudinal axis approximately 39 μm; anterior pair of ostioles absent.
Specimens of R. illicians have been previously referred to in the literature as “Ripersiella undescribed (i)” (
The species epithet is an adjective formed from illicium, meaning attraction or enticement, and its use alludes to the apparent tendency of Acropyga colonies to gain rhizoecid partners through horizontal acquisitions. Such colonies have been figuratively attracted away from their primary associates in Xenococcidae.
Ripersiella illicians sp. nov. is similar to R. colombiensis as both species have a subcircular body shape, bearing one circulus, and both are lacking multilocular disc pores and an anterior pair of dorsal ostioles. However, R. illicians can be distinguished from R. colombiensis as follows (character states for R. colombiensis are given in parentheses): having 6-segmented antennae (5-segmented antennae); having comparatively long body setae, ranging from 15–40 μm (comparatively short, ranging from 15–25 μm); and having anal lobe setae that are distinctly longer than body setae, 57–60 μm (similar in length to body setae, 18–28 μm).
Ripersiella illicians was discovered from a large nest of Acropyga goeldii (group). Root mealybugs were abundant in the nest, and wax could be seen on their body using a hand lens. Their direct association was confirmed through observation of the colony using a nest-box, as described by
Holotype. Dominican Republic • 1 adult ♀; W. of Hondo Valley, 13 m off road; 18.7229, -71.7061, elev. 1032 m; 24.vii.2009; JS LaPolla, SA Schneider leg.; associated with Acropyga dubitata, nest under a stone in coffee plantation next to road, host Coffea sp.;
Adult female (N = 9). In life, body bright white to cream colored and free of obvious waxy secretions, small deposits of wax from trilocular pores visible under SEM (Fig.
Ripersiella montanae Schneider & LaPolla sp. nov. Scanning electron micrograph (SEM) by J. Mowery (USDA ARS SEL). Ventral view of adult female labium and prothoracic legs, showing curled wax deposits and an abundance of domed dermal micro-bumps surrounding the attachment points of appendages.
General. Mounted on microscope slide, body broadly oval and membranous, 0.91–1.03 (0.99) mm long, widest near abdominal segments III–V, 0.51–0.68 (0.62) mm wide. Abdomen slightly constricted between segments VII and VIII or smoothly tapering; abdominal segment VIII 199–250 (199) μm wide at base. Anal lobes poorly developed with several flagellate setae on venter and dorsum, ranging from 16–59 μm long, with longest setae situated near dorsal margin; lacking differentiated set of 3 longer stout setae common to the group. Body setae short and flagellate, 10–22 μm on head, 12–20 μm on thorax, 14–24 μm on abdominal segments. Trilocular pores abundant and distributed among body setae; bitubular cerores absent; oral collar tubular ducts absent. Microtrichia present on abdominal segments; rounded dermal micro-bumps abundant in intersegmental areas and surroundings of appendages (Fig.
Venter. Cephalic plate absent. Labium with 3 segments; 70 μm long and 42 μm at widest point. Antennae geniculate, 5-segmented, closely situated near midline on ventral submargin of head; overall length 126–133 (129) μm; length of segment I: 30–34 (34) μm; segment II: 12–19 (13) μm; segment III: 20–26 (24) μm; segment IV: 17–21 (18) μm; segment V: 36–42 (40) μm; apical antennal segment with 1 spine-like and 4 falcate stout sensory setae; a few flagellate setae on each antennal segment, 20–30 μm long; sensorium present on second antennal segment. Legs well developed; overall length of hind leg 253–277 (275) μm; length of hind coxa 32–48 (48) μm; hind trochanter + femur 86–101 (95) μm; hind tibia + tarsus 101–111 (111) μm; hind claw 21–26 (21) μm; each claw with short setose digitule 2–3 μm long; flagellate or stout setae present on each segment, about 25 μm long. Circulus absent. Multilocular disc pores in irregular rows or groups on segments VI–VIII, sometimes absent from segment VI, pores with 7 loculi in the outer ring.
Dorsum. Anal ring on dorsal surface separated from posterior body margin by approximately one-half diameter of ring, 40–53 μm in diameter; with oval cells, some cells bearing spicules; typically with three pairs of setae, although one specimen in type series (JSL090724-05E) has four pairs, each 40–50 μm long. Posterior pair of dorsal ostioles present, diameter of orifice measured along longitudinal axis approximately 40 μm; anterior pair present but barely perceptible except under SEM, reduced compared to posterior pair and poorly developed, diameter of orifice approximately 15 μm. Multilocular disc pores absent.
Specimens of R. montanae have been previously referred to in the literature as “Rhizoecus new sp.” (
The species epithet is derived from the Latin adjective montanus (of mountains), indicating that the type series was collected from a mountainous area of Hispaniola.
Ripersiella montanae sp. nov. is similar in appearance to R. campensis sp. nov. The diagnosis section under R. campensis explains how the two species may be distinguished. Ripersiella montanae is also similar in appearance to R. andensis but the two species can be distinguished as follows (character states for R. andensis are given in parentheses): having antennal segments II–IV differing in length (subequal in length); having a comparatively short terminal antennal segment, 40 μm long (comparatively long, 53 μm); having hind legs approximately 275 μm long (240 μm); having the hind trochanter+femur shorter than the tibia+tarsus (the reverse); and having anal lobe setae as long as 59 μm (as long as 33 μm).
Ripersiella montanae was discovered from two nests of A. dubitata (nests DR1–2). The nests were located under stones in a coffee plantation within a few meters of one another. We verified direct species-to-species association (trophobiosis) between the scale insects and ants through observation of attendance by worker ants and by the fact that both colonies contained numerous individuals of the same root mealybug species within their nest chambers and no additional species of scale insects were present. This species was only discovered at high elevation (>1000 m) in the mountainous region of western Dominican Republic near the border with Haiti. Several nests of A. dubitata were collected throughout the lowland regions (112–290 m) of Dominican Republic but these nests contained a different associated root mealybug species, R. campensis.
Holotype. Peru • 1 adult ♀; Madre de Dios, Dept. Cusco, Cosnipata Valley, Carretera a Manu; -13.0685, -71.5539; 3.viii.2012; TR Schultz leg. (TRS120803-05); collected from Acropyga goeldii (group) colony; host not recorded;
Adult female (N = 3). Appearance in life not recorded.
General. Mounted on microscope slide, body membranous, broadly oval in young adults to nearly circular in more mature specimens, 1.31–1.53 (1.43) mm long, widest near abdominal segments III–V, 1.01–1.44 (1.21) mm wide. Abdomen smoothly rounded; abdominal segment VIII 258–275 (258) μm wide at base. Anal lobes poorly developed with several flagellate setae on venter and dorsum, ranging from 10–40 μm long, each with group of 3 distinctly stouter setae situated near dorsal margin 71–108 μm long. Body setae flagellate, 11–33 μm on head, 11–15 μm on thorax, 10–40 μm on abdominal segments. Trilocular pores abundant and distributed among body setae; bitubular cerores absent. Microtrichia present; presence of rounded dermal micro-bumps uncertain. Eyes absent.
Venter. Cephalic plate present, with few setae on the plate and several setae and trilocular pores surrounding the ventral and lateral margins. Labium with 3 segments; 92 μm long and 49 μm at widest point. Antennae geniculate, 5-segmented, closely situated near midline on ventral submargin of head; overall length 188–201 (192) μm; length of segment I: 45–50 (48) μm; segment II: 19–24 (19) μm; segment III: 19–21 (19) μm; segment IV: 19–23 (23) μm; segment V: 79–88 (79) μm; apical antennal segment with 1 spine-like and 4 falcate stout sensory setae; a few flagellate setae on each antennal segment, 30–45 μm long; sensorium present on second antennal segment. Legs well developed; overall length of hind leg 346–381 (346) μm; length of hind coxa 42–63 (42) μm; hind trochanter + femur 132–137 (132) μm; hind tibia + tarsus 136–144 (136) μm; hind claw 36–37 (36) μm; each claw with short setose digitule 7.5 μm long; flagellate setae present on each segment, about 25 μm long; 3 stout spine-like setae on inner margin of tibia and tarsus. Circulus absent. Multilocular disc pores present near the vulva on abdominal segments VII–VIII with 9 loculi in the outer ring; near each spiracle a multilocular disc pore with 6 or 7 loculi present. Oral collar tubular ducts present in singular rows or sparsely scattered on median to submedian areas of ventral abdominal segments, 1 or 2 present on thoracic segments near each spiracle.
Dorsum. Anal ring on dorsal surface separated from posterior body margin by approximately 1× diameter of ring, 66–73 μm in diameter; with oval cells, some cells bearing spicules; with three pairs of setae 30–42 μm long. Posterior pair of dorsal ostioles present, diameter of orifice measured along longitudinal axis approximately 44 μm; anterior pair present but smaller than posterior pair, diameter of orifice approximately 29 μm. Multilocular disc pores absent. Oral collar tubular ducts absent.
The species epithet is an adjective meaning “from the foot of the Andes”, which combines the noun pedis (foot), the adjective andinus (pertaining to the Andes Mountains), and suffix -ensis (of or from a place).
Ripersiella pediandensis sp. nov. is similar in appearance to R. andensis but the two species can be distinguished as follows (character states for R. andensis are given in parentheses): having oral collar tubular ducts present on the venter (absent); having comparatively long claws, 37 μm (comparatively short, 24 μm); having comparatively long anal lobe setae, 71–108 μm (comparatively short, 33 μm). The body and appendages of R. pediandensis are also longer in comparison. Compared to R. kelloggi (character states in parentheses), the legs of R. pediandensis are larger in proportion to the body (smaller in proportion), the cephalic plate is present (absent), multilocular disc pores are present near the vulva (absent), it is lacking a circulus (bears 2 small circuli), and the longest anal lobe setae are 71–108 μm long (less than 30 μm long). See the diagnosis under R. telalia sp. nov. for a comparison to that species.
The association between R. pediandensis and a species of Acropyga (within the goeldii group) is lacking information on observations that were made to confirm direct trophobiosis between these partners, and only a single nest was collected. Thus, as in some other cases discussed by
Holotype. Peru • 1 adult ♀; Madre de Dios, Manu National Park, Cocha Cashu Biological Station, trail intersection of 1:306 and 5A; -11.8833, -71.4000; 10.vi.2019; JS LaPolla, SA Schneider leg.; from large Acropyga (possibly) decedens nest;
Adult female (N = 7). In life, body bright white to cream colored and visibly coated in powdery white wax (Fig.
Ripersiella telalia Schneider sp. nov. Scanning electron micrograph (SEM) by J. Mowery (USDA ARS SEL). Dorsal anterior view of adult female head and thorax, showing the presence of anterior ostioles, relatively heavy coating of curled wax deposits, and apparent absence of domed dermal micro-bumps in intersegmental areas.
General. Mounted on microscope slide, body broadly oval and membranous, 0.85–1.07 (0.95) mm long, widest near abdominal segment III, 0.55–0.72 (0.65) mm wide. Abdomen smoothly tapering toward posterior end; abdominal segment VIII about 250 μm wide at base. Anal lobes poorly developed with several stout flagellate setae on venter and dorsum, 16–25 μm long, with longest setae on margin; lacking differentiated set of 3 longer stout setae common to the group. Body setae short and flagellate, 9–12 μm on head, 8–12 μm on thorax, 9–19 μm on abdominal segments. Trilocular pores abundant and distributed among body setae; bitubular cerores absent; 1–4 oral collar tubular ducts present on margins of each abdominal segment VI–VIII and extending as far anterior as III on some specimens. Microtrichia present; rounded dermal micro-bumps apparently absent. Eyes absent.
Venter. Cephalic plate present. Labium with 3 segments; 76 μm long and 39 μm at widest point. Antennae geniculate, 5-segmented, closely situated near midline on ventral submargin of head; overall length about 126–129 (128) μm; length of segment I: 32–35 (32) μm; segment II: 13–16 (16) μm; segment III: 14–17 (15) μm; segment IV: 12–14 (13) μm; segment V: 50–52 (52) μm; on some specimens apical antennal segment retains partial intersegmental line marking obsolete segment VI; with 1 spine-like and 3 falcate stout sensory setae on apical segment; a few flagellate setae on each antennal segment, 15–30 μm long; sensorium present on second antennal segment. Legs well developed; overall length of hind leg 267–284 (270) μm; length of hind coxa 41–52 (41) μm; hind trochanter + femur 101–105 (102) μm; hind tibia + tarsus 97–101 (101) μm; hind claw 26–30 (28) μm; each claw with short setose digitule 2–3 μm long; flagellate setae present on each segment, around 13–20 μm long; 3 stout spine-like setae on inner margin of tibia and tarsus. Circulus absent. Multilocular disc pores in irregular rows or groups on median to submedian of III–VIII, sparse on III–VI, abundant on segments VII and VIII with some located on submargins, pores with 6 or 7 loculi in the outer ring.
Dorsum. Anal ring near dorsal margin, 41–46 μm in diameter; with oval cells, some cells bearing spicules; with 3 pairs of setae 26 μm long. Posterior pair of dorsal ostioles present, diameter of orifice measured along longitudinal axis approximately 42 μm; anterior pair present and slightly smaller than posterior pair, diameter of orifice approximately 33 μm. Multilocular disc pores absent.
Specimens of R. telalia have been previously referred to in the literature as “Ripersiella near andensis (ii)” (
The species epithet is a genitive noun meaning “distant conversation”, combining the Greek noun lalia (conversation, talk) with the suffix tele (at a distance). Throughout the isolation of the modern pandemic, quarantine chat groups helped us maintain a much-needed sense of community. This species is named in tribute to these groups, and is specifically dedicated to Josh, Justin, and Mark. The name can be considered a double entendre, as it also alludes to the species’ symbiosis with ants as an ongoing conversation between distantly related partners.
Ripersiella telalia sp. nov. is similar to R. pediandensis sp. nov. in possessing oral collar tubular ducts, but the distribution of ducts differs between species. In R. telalia these ducts are restricted to the margins of abdominal segments with only 1–4 present per segment, whereas in R. pediandensis they are present in ventral bands often exceeding four per segment. The anterior pair of dorsal ostioles are large and more obviously developed in R. telalia relative to the other species discussed in this work.
Ripersiella telalia was discovered from quite a large nest of Acropyga (probably) decedens. We estimated in the field that the nest comprised thousands of individual ants and scales. Their direct association was confirmed through observation of the colony using a nest-box, as described by
In
1 | Ventral multilocular disc pores absent | 2 |
– | Ventral multilocular disc pores present | 4 |
2 | Oral collar tubular ducts present; abdomen bearing 2 circuli | R. kelloggi Ehrhorn & Cockerell |
– | Oral collar tubular ducts absent; abdomen bearing 1 circulus | 3 |
3 | Antennae 5-segmented, about 180 μm long; metathoracic legs about 350 μm long; anal lobe setae short, 18–28 μm | R. colombiensis (Hambleton) |
– | Antennae 6-segmented, about 135 μm long; metathoracic legs about 285 μm long; anal lobe setae long, 57–60 μm | R. illicians Schneider & LaPolla sp. nov. |
4 | Oral collar tubular ducts absent | 5 |
– | Oral collar tubular ducts present | 7 |
5 | Antennal segment V shorter than combined length of segments II–IV; antennal segments II–IV differing in length | R. montanae Schneider & LaPolla sp. nov. |
– | Antennal segment V equal to or exceeding combined length of segments II–IV; antennal segments II–IV subequal in length | 6 |
6 | Ventral multilocular disc pores present on abdominal segments III, IV, or V; longest anal lobe setae distinctly longer than body setae, up to 75 μm long | R. campensis Schneider & LaPolla sp. nov. |
– | Ventral multilocular disc pores always absent from segments III–IV and rarely on V; longest anal lobe setae similar in length to body setae, up to 35 μm long | R. andensis (Hambleton) |
7 | Oral collar tubular ducts present on medial and submedial areas of abdominal segments; longest anal lobe setae distinctly longer than body setae | R. pediandensis Schneider & LaPolla sp. nov. |
– | Oral collar tubular ducts restricted to submarginal and marginal areas of abdominal segments; longest anal lobe setae similar in length to body setae | R. telalia Schneider sp. nov. |
With the inclusion of several new species in this complex, we can begin to recognize shared traits that may relate to their intimate association with Acropyga ants. Structures relating to wax production are of interest. Species in the andensis-complex are all conspicuously lacking tubular cerores, which is an uncommon trait among the Rhizoecidae (
The trend toward reduction of ostioles among the andensis-complex appears to be related to ant association as well. The anterior pair of dorsal ostioles is reduced in size compared to the posterior pair or they are lost entirely among species in the complex. We also note a gradient in the degree of ostiole development among species, ranging from R. telalia (Figs
Finally, the “hairiness” of species seems potentially important among groups of trophobiotic mealybugs. For example, members of Xenococcidae tend to be densely covered in setae and/or microtrichia (
We thank Dr Roxana Arauco Aliaga for accommodation at Cocha Cashu Biological Station and for assistance with permitting (SERFOR #003620). We thank Kelvin Guerrero for assisting with travel, accommodations, and field work in the Dominican Republic. Thanks to Joe Mowery (USDA ARS SEL) for assistance with SEM imaging. And we thank Imre Foldi for suggesting terminology to describe the unfamiliar domed intersegmental dermal structures visible under SEM. Éva Szita, Hirotaka Tanaka, and Alejandro Caballero kindly provided reviews, and their suggestions helped to improve this manuscript. Support for this research was provided by the National Science Foundation (award number 1754242 to JSL and SAS; DEB-0743542 to JSL). The research was supported also in part by the U.S. Department of Agriculture, Agricultural Research Service. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture; USDA is an equal opportunity provider and employer.
The authors have declared that no competing interests exist.