Research Article |
Corresponding author: Hao Yu ( sacspider@126.com ) Corresponding author: Yucheng Lin ( linyucheng@scu.edu.cn ) Academic editor: Jeremy Miller
© 2022 Weicheng Yang, Hao Yu, Yucheng Lin.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Yang W, Yu H, Lin Y (2022) A review of the spider genus Chthonopes (Araneae, Theridiosomatidae), with descriptions of two new species from China. ZooKeys 1124: 147-160. https://doi.org/10.3897/zookeys.1124.89991
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The genus Chthonopes Wunderlich, 2011 is reviewed in this paper. The type species Chthonopes jaegeri Wunderlich, 2011 was illustrated based on new material from the type locality and the new distribution records (Bolikhamsay and Ban Kouanphavang Khammouane, Laos). Two new species are described from Yunnan, China: C. bifidum Yu & Lin, sp. nov. (♂♀) and C. jimudeng Yu & Lin, sp. nov. (♀). A key is provided for the genus, as well as species diagnoses, and a distribution map for all five species of Chthonopes.
Araneoidea, Asia, key, revision, theridiosomatid, Yunnan
Theridiosomatidae Simon, 1881 is a small-sized spider family with 20 genera and 135 valid species distributed worldwide, with 11 genera and 28 species recorded from China (
The genus Chthonopes was originally erected by
While studying material from Yunnan Province, China, we recognized several specimens belonging to Theridiosomatidae. Detailed study of these specimens reveals that they belong to two undescribed species of Chthonopes, a genus previously unknown in China. The goals of this paper are 1) to describe the two new species under the names of C. bifidum Yu & Lin sp. nov. and C. jimudeng Yu & Lin sp. nov.; 2) to re-illustrate C. jaegeri based on new material from Laos, and give supplementary micrographs; and 3) to conduct a comprehensive review of the genus Chthonopes, including an identification key and a distribution map for all species.
Specimens were examined and measured with a Leica M205 C stereomicroscope. Further details were studied with an Olympus BX43 compound microscope. Male and female copulatory organs were examined after they were dissected and detached from the bodies. Epigyne were removed and treated with lactic acid before photographed. All specimens were preserved in 95% ethanol. Photos were taken with a Canon EOS 60D wide zoom digital camera (8.5 megapixels) mounted on an Olympus BX43 stereomicroscope. The images were montaged using Helicon Focus ver.3.10 (
The distribution map was generated with ArcGis ver.10.5 (Environmental Systems Research Institute, Inc.). Locality coordinates for all species are copied from the original publications (see
Abbreviations used in the text and figures are as follows:
Asp accessory spermathecae;
CD copulatory duct;
CL cymbial lobe;
Co conductor;
DA distal apophysis on tegulum;
DH distal horn on median apophysis;
ED embolic distal end;
Em embolus;
FD fertilization duct;
MA median apophysis;
Pc paracymbium;
Sc scape;
Sp spermathecae;
St subtegulum;
Te tegulum;
TTr tibial trichobothium.
All examined materials are deposited in the Natural History Museum of Sichuan University in Chengdu (
Chthonopes jaegeri Wunderlich, 2011 from Bolikhansay, Laos, by original designation.
Chthonopes species can be recognised by the copulatory organs: In males, the cymbium apically-ventrally bearing several setae or hairs; median apophysis large and flat, located at the basal or subbasal portion of the tegulum, distally bearing a horn; bulb with an erect distal apophysis located on the apical part of the tegulum; embolus long, accompanied by a tubular conductor, embolic distal end forked. In females, the epigynal plate possesses a scape; vulval center with a V-shaped medial structure; copulatory ducts long, proximally thin but thick-walled, extending anteriorly along flanks of the V-shaped structure, the latter half wide and forming two egg-shaped bursae, surface membranous, wrinkled and ribbed, then connecting with main spermathecae at the central axis of the vulva; main spermathecae small, strongly sclerotized, globular or reniform, separated by about 0.1 – 1.2× their width; hyaline accessory spermathecae located laterally or anterolaterally to main spermathecae, usually claviform or tubular.
See
Chthonopes cavernicola Wunderlich, 2011 (♂), C. jaegeri Wunderlich, 2011 (♂♀) and C. thakekensis Lin, Li & Jäger, 2014 (♀) from Laos, C. bifidum sp. nov. (♂♀) and C. jimudeng sp. nov. (♀) endemic to China.
1 | Males | 2 |
– | Females | 4 |
2 | Anterior eye row with 6 eyes, posterior eye row with 2 eyes; cymbium apically bearing four tiny hairs which are not situated on a hump ( |
C. cavernicola |
– | Both anterior and posterior eye rows with 4 eyes; cymbium apically-ventrally with a pair of long and bristle-shaped hairs on a hump (Figs |
3 |
3 | Distal horn on median apophysis (DH) bifurcate (Fig. |
C. bifidum sp. nov. |
– | Distal horn on median apophysis (DH) represented by a small needle or spine, not forked, (Fig. |
C. jaegeri |
4 | Scape (Sc) long, more than 1/2 of epigyne length, rugose (Fig. |
5 |
– | Scape (Sc) short, about 1/5 of epigyne length, not rugose (Fig. |
6 |
5 | Main spermathecae (Sp) semi-circular, separated by about 1.2× their width; accessory spermathecae (Asp) claviform; fertilization ducts (FD) membranous ( |
C. thakekensis |
– | Main spermathecae (Sp) circular, separated by about 1/3 of their diameter; accessory spermathecae (Asp) consisting of tubular stalk and globular head; FD strongly sclerotized (Fig. |
C. jaegeri |
6 | Scape (Sc) triangular, translucent, extending from posterior margin of epigynal plate; accessory spermathecae (Asp) located anterolaterally to main spermathecae, nearly claviform or tubular (Fig. |
C. bifidum sp. nov. |
– | Scape (Sc) digitiform, relatively sclerotized, originating from dorsal side of posterior margin of epigynal plate; accessory spermathecae (Asp) located laterally to main spermathecae, consisting of tubular stalk and globular head (Fig. |
C. jimudeng sp. nov. |
Holotype : ♂, China: Yunnan Province: Xishuangbanna: Mengla County: Menglun Town: Shenmi cave, 21.97°N, 101.24°E, elevation 776 m, 3.X.2017, Y. Lin and Y. Li leg. Paratypes: 15♀11juv., same data as holotype.
1♂ 24♀ 1juv., China: Yunnan Province: Dehong: Luxi City: Mangliu village: Xianfo cave, 24.33°N, 98.52°E, elevation 1081 m, 25.VIII.2010, C. Wang leg.
The species epithet is taken from the Latin adjective “bifidus” and refers to the forked distal horn of median apophysis.
The males of C. bifidum sp. nov. easily differentiated from those of all other congeners by the bifurcate distal horn of median apophysis, the partly membranous or hyaline, whisker-shaped distal apophysis of tegulum, and by the paracymbium with a spine-like tip, vs. distal horn on median apophysis represented by a small needle or spine, not forked, distal apophysis on tegulum relatively sclerotized, lamina-shaped, paracymbium without spine-like tip in C. jaegeri and C. cavernicola (cf. Fig.
Male (holotype) (Fig.
Chthonopes bifidum sp. nov., male holotype and female paratype, male habitus (A, B), female habitus (C, D) and epigyne (E–G) A dorsal view B ventral view C dorsal view D ventral view E intact, ventral view F cleared, ventral view G cleared, dorsal view. Abbreviations: Asp = accessory spermathecae; CD = copulatory duct; FD = fertilization duct; Sc = scape; SP = spermatheca. Scale bars: 0.5 mm (A–D); 0.2 mm (E, F, G).
Palp
(Fig.
Male palp of the holotype of Chthonopes bifidum sp. nov. A prolateral view B retrolateral view C ventral view D dorsal view. Abbreviations: Co = conductor; CL = cymbial lobe; DA = distal apophysis on tegulum; DH = distal horn on median apophysis; ED = embolic distal end; Em = embolus; MA = median apophysis; Pc = paracymbium; St = subtegulum; Te = tegulum; TTr = tibial trichobothium. Scale bars: 0.2 mm (A–D).
Female (one paratype). Somatic features as in Fig.
Epigyne
(Fig.
Known from Mengla County and Luxi City, Yunnan, China (Fig.
Chthonopes cavernicolus Wunderlich, 2011: 433, figs 8–18 (♂).
Not examined.
See diagnosis for C. jaegeri.
See
Laos (Fig.
Chthonopes jaegeri Wunderlich, 2011: 435, fig. 18a–f (♂♀).
2♀, Laos: Khammouan Province: Thakek area, Ban Phôungam-Mai, 17.55°N, 104.81°E, elevation 495 m, 25.XI.2012, S. Li leg; 7♀, Bolikhamxay Province: Khamkeut area, 18.22°N, 104.81°E, elevation 495 m, 27.XI.2012, Z. Yao leg; 1♂ 2♀, Bolikhamxay Province: Lak Sao, 17.22°N, 104.81°E, elevation 501 m, 3.III.2010, H. Steiner leg.
Chthonopes jaegeri, male habitus (A, B), female habitus (C, D) and epigyne (E–G) A dorsal view B ventral view C dorsal view D ventral view E intact, ventral view F cleared, ventral view G cleared, dorsal view. Abbreviations: Asp = accessory spermathecae; CD = copulatory duct; FD = fertilization duct; Sc = scape; SP = spermatheca. Scale bars: 0.5 mm (A–D); 0.2 mm (E, F, G).
The male of C. jaegeri resembles those of C. cavernicola (Wunderlich, 2011: 433, figs 8–18) in having a large and flat, laminar median apophysis which bears a tiny needle-shaped distal horn (Fig.
Male palp of Chthonopes jaegeri A prolateral view B retrolateral view C ventral view D dorsal view. Abbreviations: Co = conductor; CL = cymbial lobe; DA = distal apophysis on tegulum; DH = distal horn on median apophysis; ED = embolic distal end; Em = embolus; MA = median apophysis; Pc = paracymbium; St = subtegulum; Te = tegulum. Scale bars: 0.2 mm (A–D).
See
Laos (Fig.
Holotype : ♀, China: Yunnan Province: Gongshan County: Dulongjiang Town: Jimudeng village, 27.79°N, 98.33°E, elevation 1410 m, 15.VIII.2018, Y. Lin and Y. Li leg. Paratypes: 1♀ 2juv., same data as holotype.
6juv., China: Yunnan Province: Gongshan County: Dulongjiang Town: Maku village, 27.68°N, 98.30°E, elevation 1939 m, 14.VIII.2018, Y. Lin and Y. Li leg.
This specific name is taken from type locality; noun in apposition.
The new species is similar to C. bifidum sp. nov. (Fig.
Holotype female of Chthonopes jimudeng sp. nov., habitus (A, B) and epigyne (C–E) A dorsal view B ventral view C intact, ventral view D cleared, ventral view E cleared, dorsal view. Abbreviations: Asp = accessory spermathecae; CD = copulatory duct; FD = fertilization duct; Sc = scape; SP = spermatheca. Scale bars: 0.5 mm (A, B); 0.2 mm (C, F, G).
Female (holotype) (Fig.
Epigyne
(Fig.
Unknown.
Known only from Gongshan County, Yunnan, China (Fig.
Distribution records of five species of the genus Chthonopes: C. bifidum sp. nov. (scarlet circle: 1. China, Yunnan Province, Xishuangbanna, Mengla County, Menglun Town, Shenmi cave; 2. China, Yunnan Province, Dehong, Luxi City, Mangliu village, Xianfo cave); C. jimudeng sp. nov. (yellow circle: 1. China, Yunnan Province, Gongshan County, Dulongjiang Town, Jimudeng village; 2. China, Yunnan Province, Gongshan County, Dulongjiang Town, Maku village); C. jaegeri Wunderlich, 2011 (carmine circle: 1, 2. Laos, Bolikhansay Province,, Lak Sao, Tham Mang Kone; 3. Laos, Khammouan Province, Thakek area, Ban Phôungam-Mai; 4. Laos, Bolikhamxay Province: Khamkeut area); C. cavernicola Wunderlich, 2011 (light blue circle: Laos, Khammouan Province, Tham Boumlou, inside cave); C. thakekensis Lin, Li & Jäger, 2014 (green circle: Laos, Khammouan Province, Thakek area, Ban Phôungam-Mai, Tham Phayat, limestone cave).
Chthonopes thakekensis Lin, Li & Jäger, 2014: 98, figs 17A–E, 18A–C (♀).
None.
See diagnosis for C. jaegeri.
See
Laos (Fig.
Sincere thanks to Dr Jonathan Coddington and the subject editor, Dr Jeremy A. Miller for constructive comments that help improve this manuscript. This study was supported by the National Natural Science Foundation of China to Yucheng Lin (NSFC-31772410, 31750002, 31972870) and Hao Yu (NSFC-32060113, 31702006), the Natural Science Foundation of Guizhou Province to Hao Yu ([2020]1Y081), the Guizhou Science and Technology Support Program ([2017]2811) and the Forestry Science and Technology Research Program of Guizhou Forestry Department ([2022]27) to Weicheng Yang.