Research Article |
Corresponding author: Dingqi Rao ( raodq@mail.kiz.ac.cn ) Corresponding author: Natalia B. Ananjeva ( natalia_ananjeva@yahoo.com ) Academic editor: Johannes Penner
© 2022 Shuo Liu, Mian Hou, Dingqi Rao, Natalia B. Ananjeva.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Liu S, Hou M, Rao D, Ananjeva NB (2022) Three new species of Diploderma Hallowell, 1861 (Squamata, Agamidae) from the Hengduan Mountain Region, south-western China. ZooKeys 1131: 1-30. https://doi.org/10.3897/zookeys.1131.86644
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Three new species of Diploderma are described from the Hengduan Mountain Region in south-western China, based on morphological and genetic data. The first new species from Yulong County, Yunnan Province is morphologically most similar and phylogenetically closely related to D. brevicauda, but it can be diagnosed from the latter by having a relatively longer tail; the second new species from Xiangcheng County, Sichuan Province is phylogenetically closely related to D. bowoense, but it can be diagnosed from the latter by the absence of a distinct gular spot; and the third new species from Yongsheng County, Yunnan Province is phylogenetically closely related to D. yulongense, but it can be diagnosed from the latter by having different colourations of the ventral and ventrolateral surfaces of the body. Taxonomy and diversity survey are the basis of species conservation, our discoveries contributing to better conservation of the species of this genus.
Molecular, morphological, ND2, Sichuan, taxonomy, Yunnan
Diploderma Hallowell, 1861, is a genus including 36 species recognised currently (
In the Hengduan Mountain Region, species of Diploderma mainly inhabit the hot-dry river valleys and most species are micro-endemic and only found in a specific section of a given river valley (
During our field survey in the Hengduan Mountain Region, China, in April 2022, some specimens of Diploderma were collected from the middle Jinsha River Valley in Yongsheng County, the area nearby the upper Jinsha River in Yulong County and the valley of a tributary of the upper Jinsha River in Xiangcheng County in Yunnan and Sichuan provinces, respectively (Fig.
Map showing the type localities of Diploderma limingense sp. nov. (black triangle), Diploderma shuoquense sp. nov. (black dot) and Diploderma yongshengense sp. nov. (black square) in the Hengduan Mountain Region, south-western China. The elevation data were obtained from
Specimens were all collected during the day. Photographs were taken to document the colour pattern in life prior to euthanasia. Liver tissues were stored in 99% ethanol and lizards were preserved in 75% ethanol. Specimens were deposited at Kunming Natural History Museum of Zoology, Kunming Institute of Zoology, Chinese Academy of Sciences (
Specimens were measured using a digital caliper to the nearest 0.1 mm. Measurements were taken on the left side of the specimen and values for paired pholidosis characters were recorded on both sides of the body, with counts provided in left/right order. The following morphometric characters were measured following
F4S fourth finger subdigital lamellae number, subdigital lamellae scale from the base between third and fourth finger to the tip of fourth finger, excluding the claw;
FLL fore-limb length, measured between the point of insertion at axillary to the tip of fourth finger, excluding the claw, measured as the straightened limb;
HD head depth, measured as the perpendicular distance at the temporal region of head;
HL head length, measured from the tip of snout to the rear border of the angle of jaw;
HLL hind-limb length, measured between the point of insertion at groin to the tip of fourth toe, excluding the claw, measured as the straightened limb;
HW head width, measured between the widest points of the head;
IL infralabial scale number, enlarged, modified labial scales from mental to the corner of mouth;
MD mid-dorsal crest scale number, modified crest scales longitudinally from the first nuchal crest to the scale above cloaca;
NSL nasal-supralabials scale rows, number of horizontal rows of small scales between the first supralabial and the nasal;
SEL snout-eye length, measured between the tip of snout and anterior edge of orbital bone;
SL supralabial scale number, enlarged, modified labial scales from rostral to the corner of mouth;
SOR suborbital scale rows, longitudinal rows of scales between supralabials and inferior-most edge of orbit circle, excluding fine ciliary scales in the orbit;
SVL snout-vent length, measured from the snout tip to anterior edge of the cloaca;
T4L fourth toe length, measured between the tip of fourth toe to the base between third and fourth toe, excluding the claw;
T4S fourth toe subdigital lamellae number, subdigital lamellae scales from the base between third and fourth toe to the tip of fourth toe, excluding the claw;
TAL tail length, measured from the anterior edge of the cloaca to the tip of tail;
TRL trunk length, measured between the limb insertion points between axillary and groin;
VN ventral scale number, ventral body scales counted in a straight line along the medial axis between the transverse gular fold and the anterior edge of cloaca.
We compared morphological characters of the new species with other members of the genus relying on original species descriptions (
Total genomic DNA for the new collected specimens was extracted from liver tissues with the standard extraction method (
Species | Voucher | Locality | Accession Numbers |
---|---|---|---|
Diploderma angustelinea |
|
Muli, Sichuan, China | MT577930 |
Diploderma angustelinea |
|
Muli, Sichuan, China | MT577924 |
Diploderma aorun |
|
Benzilan, Yunnan, China | MT577938 |
Diploderma aorun |
|
Benzilan, Yunnan, China | MT577939 |
Diploderma batangense |
|
Zhubalong, Tibet, China | MK001412 |
Diploderma batangense |
|
Batang, Sichuan, China | MK001413 |
Diploderma brevicauda |
|
Lijiang, Yunnan, China | MW506023 |
Diploderma brevicauda |
|
Lijiang, Yunnan, China | MW506021 |
Diploderma brevicauda |
|
Lijiang, Yunnan, China | MW506022 |
Diploderma bowoense |
|
Muli, Sichuan, China | MW506020 |
Diploderma bowoense |
|
Muli, Sichuan, China | MW506019 |
Diploderma brevipes | NMNS 19607 | Taiwan, China | MK001429 |
Diploderma brevipes | NMNS 19608 | Taiwan, China | MK001430 |
Diploderma chapaense |
|
Lvchun, Yunnan, China | MG214263 |
Diploderma chapaense | ZMMU NAP-01911 | Chapa, Vietnam | MG214262 |
Diploderma drukdaypo |
|
Jinduo, Tibet, China | MT577950 |
Diploderma drukdaypo |
|
Zhuka, Tibet, China | MT577952 |
Diploderma dymondi |
|
Dongchuan, Yunnan, China | MK001422 |
Diploderma dymondi |
|
Dongchuan, Yunnan, China | MK001423 |
Diploderma flaviceps |
|
Luding, Sichuan, China | MK001416 |
Diploderma flaviceps |
|
Luding, Sichuan, China | MK001417 |
Diploderma flavilabre |
|
Baiyu,Sichuan, China | MT577916 |
Diploderma flavilabre |
|
Baiyu,Sichuan, China | MT577917 |
Diploderma formosgulae |
|
Deqin, Yunnan, China | MW506024 |
Diploderma formosgulae |
|
Deqin, Yunnan, China | MW506025 |
Diploderma iadinum |
|
Yunling, Yunnan, China | MT577956 |
Diploderma iadinum |
|
Yunling, Yunnan, China | MT577957 |
Diploderma laeviventre |
|
Basu, Tibet, China | MK001407 |
Diploderma laeviventre |
|
Basu, Tibet, China | MT577892 |
Diploderma luei | NMNS 19604 | Taiwan, China | MK001433 |
Diploderma luei | NMNS 19605 | Taiwan, China | MK001434 |
Diploderma makii | NMNS 19609 | Taiwan, China | MK001431 |
Diploderma makii | NMNH 19610 | Taiwan, China | MK001432 |
Diploderma menghaiense |
|
Menghai, Yunnan, China | MT598655 |
Diploderma menghaiense |
|
Menghai, Yunnan, China | MT598656 |
Diploderma micangshanense |
|
Shiyan, Hubei, China | MK578665 |
Diploderma micangshanense |
|
Xixia, Henan, China | MK578664 |
Diploderma panchi |
|
Yajiang, Sichuan, China | MT577946 |
Diploderma panchi |
|
Yajiang, Sichuan, China | MT577944 |
Diploderma panlong |
|
Miansha, Sichuan, China | MT577906 |
Diploderma panlong |
|
Miansha, Sichuan, China | MT577907 |
Diploderma polygonatum | NMNS 19598 | Taiwan, China | MK001427 |
Diploderma polygonatum | NMNS 19599 | Taiwan, China | MK001428 |
Diploderma qilin |
|
Balong, Yunnan, China | MT577941 |
Diploderma qilin |
|
Balong, Yunnan, China | MT577942 |
Diploderma qilin |
|
Balong, Yunnan, China | MT577943 |
Diploderma slowinskii | CAS 214906 | Gongshan, Yunnan, China | MK001405 |
Diploderma slowinskii | CAS 214954 | Gongshan, Yunnan, China | MK001406 |
Diploderma splendidum |
|
Yichang, Hubei, China | MK001418 |
Diploderma splendidum | LSUMZ 81212 | Unknown | AF288230 |
Diploderma swild |
|
Panzhihua, Sichuan, China | MN266299 |
Diploderma swild |
|
Panzhihua, Sichuan, China | MN266300 |
Diploderma swinhonis | NMNS 19592 | Taiwan, China | MK001419 |
Diploderma swinhonis | NMNS 19593 | Taiwan, China | MK001420 |
Diploderma varcoae | WK-JK 011 | Yuxi, Yunnan, China | MT577903 |
Diploderma varcoae |
|
Mengzi, Yunnan, China | MK001421 |
Diploderma vela |
|
Quzika, Tibet, China | MK001414 |
Diploderma vela |
|
Quzika, Tibet, China | MK001415 |
Diploderma yangi | SWFU 005410 | Chayu, Tibet, China | OL449603 |
Diploderma yangi | SWFU 005412 | Chayu, Tibet, China | OL449604 |
Diploderma yulongense |
|
Hutiaoxia, Yunnan, China | MT577921 |
Diploderma yulongense |
|
Hutiaoxia, Yunnan, China | MT577922 |
Diploderma yulongense |
|
Baishuitai, Yunnan, China | MT577923 |
Diploderma yulongense |
|
Xianggelila, Yunnan, China | MK001410 |
Diploderma yulongense |
|
Xianggelila, Yunnan, China | MK001411 |
Diploderma yunnanense | CAS 242271 | Baoshan, Yunnan, China | MK001408 |
Diploderma yunnanense |
|
Yingjiang, Yunnan, China | MK578658 |
Diploderma zhaoermii |
|
Wenchuan, Sichuan, China | MK001425 |
Diploderma zhaoermii |
|
Wenchuan, Sichuan, China | MK001426 |
Diploderma limingense sp. nov. | KIZ2022013 | Liming, Yunnan, China | OP428781 |
Diploderma limingense sp. nov. | KIZ2022014 | Liming, Yunnan, China | OP428782 |
Diploderma limingense sp. nov. | KIZ2022015 | Liming, Yunnan, China | OP428783 |
Diploderma limingense sp. nov. | KIZ2022017 | Liming, Yunnan, China | OP428784 |
Diploderma shuoquense sp. nov. | KIZ2022004 | Xiangcheng, Sichuan, China | OP428773 |
Diploderma shuoquense sp. nov. | KIZ2022005 | Xiangcheng, Sichuan, China | OP428774 |
Diploderma shuoquense sp. nov. | KIZ2022006 | Xiangcheng, Sichuan, China | OP428775 |
Diploderma shuoquense sp. nov. | KIZ2022007 | Xiangcheng, Sichuan, China | OP428776 |
Diploderma yongshengense sp. nov. | KIZ2022008 | Yongsheng, Yunnan, China | OP428777 |
Diploderma yongshengense sp. nov. | KIZ2022009 | Yongsheng, Yunnan, China | OP428778 |
Diploderma yongshengense sp. nov. | KIZ2022010 | Yongsheng, Yunnan, China | OP428779 |
Diploderma yongshengense sp. nov. | KIZ2022011 | Yongsheng, Yunnan, China | OP428780 |
Pseudocalotes brevipes | MVZ 224106 | Vinh Phuc, Vietnam | AF128502 |
Pseudocalotes kakhiensis |
|
Gongshan, Yunnan, China | MK001435 |
Sequences were aligned using MUSCLE (
The obtained sequence alignment is 1031 bp in length. The resulting topologies from BI and ML analyses are consistent (Fig.
KIZ2022014, adult male, collected on 21 April 2022 by Shuo Liu from Liming Village, Liming Township, Yulong County, Lijiang City, Yunnan Province, China (27°2′0″N, 99°40′42″E, 2300 m elevation).
KIZ2022013, KIZ2022015, KIZ2022017, three adult males, collecting information the same as the holotype.
The specific epithet refers to Liming Township, where the new species was discovered.
Diploderma limingense sp. nov. can be diagnosed from congeners by a combination of the following morphological characteristics: (1) body size medium, SVL 55.6–56.8 mm in males; (2) tail relatively long, TAL/SVL 1.92–2.09 in males; (3) head moderately wide, HW/HL 0.71–0.74 in males; (4) limbs relatively long, FLL/SVL 0.47–0.52 in males, HLL/SVL 0.74–0.82 in males; (5) MD 45–48; (6) F4S 15–16, T4S 21–22; (7) tympanum concealed; (8) nuchal and dorsal crest scales feebly developed, no skin folds under nuchal and dorsal crest scales in males; (9) distinct transverse gular fold present; (10) ventral head and body scales strongly keeled; (11) ventral head scales heterogeneous in size; (12) gular spot present in males, yellowish-white in life; (13) dorsolateral stripes jagged in males, light yellow in life; (14) ventral surfaces of body, limbs and tail light brick red in males in life; (15) five radial stripes around the eye on each side; (16) inner lips bright yellow, tongue light orange, remaining oral cavity mostly light flesh colour in life.
Adult male, SVL 56.2 mm; tail relatively long, TAL 117.5 mm, TAL/SVL 2.09; limbs relatively long, FLL 26.5 mm on left side, FLL/SVL 0.47, HLL 41.8 mm on left side, HLL/SVL 0.74. Head relatively robust, HW/HL 0.74, HD/HW 0.85; snout moderately long, SEL/HL 0.36. Rostral elongated, bordered by five small postrostral scales; dorsal head scales heterogeneous, all strongly keeled; indistinct Y-shaped ridge on dorsal snout. Nasal oval, separated from first supralabial by single row of scales; loreals small, keeled; suborbital scale rows 4/3, keeled; canthus rostralis elongated, greatly overlapping with each other; enlarged, keeled scales forming single lateral ridge from posteroinferior eye to posterosuperior tympanum on each side; tympanum concealed under scales; SL 8/8, feebly keeled. Mental pentagonal; IL 9/9; enlarged chin shields 4/5, smooth, first one contacting IL on each side, remaining ones separated from IL by two rows of small scales; ventral head scales homogeneous in size, smooth or weakly keeled; distinct transverse gular fold present; gular pouch weakly developed.
Distinct shoulder fold present; dorsal body scales heterogeneous in size and shape, all keeled, tip pointing backwards; axillary scales much smaller than remaining dorsals; enlarged dorsal scales roughly forming four longitudinal rows from neck to pelvis on each side of body. Nuchal and dorsal crests continuous, scales of nuchal and dorsal crests approximately same in size and shape; no skin fold under nuchal and dorsal crests; MD 45. Dorsal limb scales strongly keeled, homogeneous on fore-limbs and heterogeneous on hind limbs; F4S 15/16, T4S 22/22. Ventral body scales approximately parallel, almost homogeneous, all strongly keeled, VN 63. Ventral limb scales parallel, small on fore-limbs and larger on hind limbs, all strongly keeled. Tail scales all strongly keeled, ventral tail scales larger than dorsal tail scales.
Dorsal surface of head brownish-grey. A distinct black transverse band anteriorly and an indistinct black transverse band posteriorly present between orbits on dorsal surface of head. Lateral surfaces of head brownish-grey. Five brownish-black radial stripes around eye on each side. Upper lips greyish-white. Inner lips bright yellow, tongue light orange, remaining oral cavity mostly light flesh colour.
Dorsal surface of body brown. A light yellow jagged dorsolateral stripe present from neck to pelvis on each side of body. Some brownish-black triangular patches distributed along vertebral line between dorsolateral stripes from neck to base of tail, all of which pointing posteriorly. Some yellowish-white spots scattered below dorsolateral stripe on each side of body. Dorsal surfaces of limbs greyish-brown with indistinct dark transverse bands. Dorsal surface of tail brownish-grey with some indistinct dark transverse bands.
Ventral surface of head greyish-white. A roughly triangular, yellowish-white gular spot present on posterior central part, many grey stripes forming reticulated pattern present on other region of ventral head. Ventral surfaces of body, limbs and tail light brick red with no patterns.
The variations of morphological character of the type series are provided in Table
Morphological data of the type series of Diploderma limingense sp. nov. Morphometric measurements are in mm. For measurement methods and abbreviations, see the Materials and methods section.
KIZ2022014 Holotype ♂ | KIZ2022013 Paratype ♂ | KIZ2022015 Paratype ♂ | KIZ2022017 Paratype ♂ | |
---|---|---|---|---|
SVL | 56.2 | 56.8 | 55.6 | 56.1 |
TAL | 117.5 | 110.1 | 113.5 | 107.7 |
HL | 18.0 | 18.5 | 18.0 | 18.0 |
HW | 13.3 | 13.2 | 13.2 | 13.1 |
HD | 11.3 | 11.2 | 11.0 | 11.4 |
SEL | 6.5 | 6.7 | 6.3 | 6.9 |
FLL | 26.5 | 27.5 | 28.7 | 27.6 |
HLL | 41.8 | 44.9 | 45.6 | 43.4 |
T4L | 10.7 | 10.9 | 11.2 | 9.9 |
TRL | 25.1 | 24.2 | 23.6 | 24.9 |
TAL/SVL | 2.09 | 1.94 | 2.04 | 1.92 |
SEL/HL | 0.36 | 0.36 | 0.35 | 0.38 |
HW/HL | 0.74 | 0.71 | 0.73 | 0.73 |
HD/HW | 0.85 | 0.85 | 0.83 | 0.87 |
FLL/SVL | 0.47 | 0.48 | 0.52 | 0.49 |
HLL/SVL | 0.74 | 0.79 | 0.82 | 0.77 |
TRL/SVL | 0.45 | 0.43 | 0.42 | 0.44 |
SL | 8/8 | 8/8 | 8/9 | 9/9 |
IL | 9/9 | 8/9 | 10/8 | 9/9 |
NSL | 2/1 | 1/1 | 1/1 | 1/1 |
MD | 45 | 45 | 47 | 48 |
F4S | 15/16 | 16/16 | 15/16 | 16/16 |
T4S | 22/22 | 22/22 | 22/21 | 21/21 |
SOR | 4/3 | 3/4 | 3/3 | 3/3 |
VN | 63 | 58 | 59 | 63 |
From species of Diploderma which are only distributed on East Asian islands, Diploderma limingense sp. nov. differs from D. brevipes (Gressitt, 1936), D. luei (Ota, Chen & Shang, 1998), D. makii (Ota, 1989), D. polygonatum Hallowell, 1861 and D. swinhonis (Günther, 1864) by the presence of a transverse gular fold (vs. absence).
From species of Diploderma which are distributed on mainland, but relatively distant from that of Diploderma limingense sp. nov., Diploderma limingense sp. nov. differs from D. chapaense (Bourret, 1937), D. fasciatum (Mertens, 1926), D. hamptoni (Smith, 1935), D. menghaiense Liu, Hou, Wang, Ananjeva & Rao, 2020, D. micangshanense (Song, 1987), D. ngoclinense (Ananjeva, Orlov & Nguyen, 2017) and D. yunnanense (Anderson, 1878) by the presence of a transverse gular fold (vs. absence); from D. dymondi (Boulenger, 1906), D. varcoae (Boulenger, 1918), by having concealed tympana (vs. exposed); from D. grahami (Stejneger, 1924) by having a much longer tail (TAL/SVL 1.92–2.09 vs. 1.64) and a distinct transverse gular fold (vs. feeble); and from D. splendidum (Barbour & Dunn, 1919) by having jagged dorsolateral stripes in males (vs. smooth).
From species of Diploderma which occupy distributions relatively close to that of Diploderma limingense sp. nov. in the Hengduan Mountain Region, Diploderma limingense sp. nov. differs from D. panlong Wang, Che & Siler, 2020, D. slowinskii, (Rao, Vindum, Ma, Fu & Wilkinson, 2017) and D. swild Wang, Wu, Jiang, Chen, Miao, Siler & Che, 2019 by having concealed tympana (vs. exposed); from D. angustelinea Wang, Ren, Wu, Che & Siler, 2020, D. aorun Wang, Jiang, Zheng, Xie, Che & Siler, 2020, D. bowoense, D. batangense (Li, Deng, Wu & Wang, 2001), D. flavilabre Wang, Che & Siler, 2020, D. formosgulae Wang, Gao, Wu, Dong, Shi, Qi, Siler & Che, 2021, D. iadinum (Wang, Jiang, Siler & Che, 2016), D. laeviventre (Wang, Jiang, Siler & Che, 2016), D. yangi Wang, Zhang & Li, 2022, D. yulongense and D. zhaoermii (Gao & Hou, 2002) by having a yellowish-white gular spot in males in life (vs. chartreuse, blue, green, lilac, orange or yellow); from D. drukdaypo (Wang, Ren, Jiang, Zou, Wu, Che & Siler, 2019) by having strongly keeled ventral scales of body (vs. smooth or weakly keeled); from D. flaviceps (Barbour & Dunn, 1919) by the presence of a colourful gular spot in males in life (vs. absence) and no skin fold under dorsal and nuchal crests in males (vs. strongly developed and erected); from D. panchi Wang, Zheng, Xie, Che & Siler, 2020 by having bright yellow inner lips in life (vs. inner lips flesh colour); and from D. vela (Wang, Jiang & Che, 2015) by having feebly developed crests without strongly erected crest scales or skin fold in males in life (vs. distinctively erected crest scales on continuous, well-developed skin fold).
Diploderma limingense sp. nov. is phylogenetically sister to D. qilin and D. brevicauda, but Diploderma limingense sp. nov. can be differentiated from D. qilin by having bright yellow inner lips and light orange tongue in life (vs. both inner lips and tongue light flesh colour) and from D. brevicauda by having a relatively longer tail in males (TAL/SVL 1.92–2.09 vs. 1.40–1.84) and more mid-dorsal crest scales (MD 45–48 vs. 34–43).
This species is known only from the type locality, Liming Township, Yulong County, Lijiang City, Yunnan Province, China (Fig.
All specimens were collected between 9 and 11 a.m. on the ground in coniferous and broad-leaved mixed forest and there was no water body nearby (Fig.
KIZ2022004, adult male, collected on 23 April 2022 by Shuo Liu from the Shuoqu River Valley, Qingde Town, Xiangcheng County, Ganzi Prefecture, Sichuan Province, China (28°48′50″N, 99°49′47″E, 2700 m elevation).
KIZ2022005–KIZ2022007, three adult males, collecting information the same as the holotype.
The specific epithet refers to the Shuoqu River, by which the new species was discovered.
Diploderma shuoquense sp. nov. can be diagnosed from congeners by a combination of the following morphological characteristics: (1) body size small, SVL 48.2–52.3 mm in males; (2) tail moderately long, TAL/SVL 1.87–1.97 in males; (3) limbs moderately long, FLL/SVL 0.45–0.49 in males, HLL/SVL 0.69–0.74 in males; (4) head moderately wide, HW/HL 0.72–0.74 in males; (5) MD 34–40; (6) F4S 13–16, T4S 19–21; (7) tympanum concealed; (8) nuchal and dorsal crest scales feebly developed, not distinctively erected or raised on skin folds in males; (9) distinct transverse gular fold present; (10) ventral head scales smooth or weakly keeled and ventral body scales strongly keeled; (11) ventral head scales homogeneous in size; (12) no distinct gular spot in males; (13) dorsolateral stripes jagged in males, yellowish-white or greyish-white in life; (14) 8–10 radial stripes around the eye on each side; (15) oral cavity, inner lips and tongue pink in life.
Adult male, SVL 52.3 mm; tail moderately long, TAL 98.3 mm, TAL/SVL 1.88; limbs moderately long, FLL 23.4 mm on left side, FLL/SVL 0.45, HLL 36.6 mm on left side, HLL/SVL 0.70. Head relatively robust, HW/HL 0.74, HD/HW 0.82; snout relatively short, SEL/HL 0.34. Rostral rectangular, bordered by six small postrostral scales; dorsal head scales heterogeneous, all strongly keeled; indistinct Y-shaped ridge on dorsal snout. Nasal oval, separated from first supralabial by single row of scales; loreals small, keeled; suborbital scale rows 3/4, keeled; canthus rostralis elongated, greatly overlapping with each other; enlarged, keeled scales forming single lateral ridge from posteroinferior eye to posterosuperior tympanum on each side; tympanum concealed under scales; SL 10/10, feebly keeled. Mental pentagonal; IL 9/9; enlarged chin shields 6/5, smooth, first one contacting IL on left side and first two contacting IL on right side, remaining ones separated from IL by one or two rows of small scales; ventral head scales homogeneous in size, smooth or weakly keeled; distinct transverse gular fold present; gular pouch weakly developed.
Distinct shoulder fold present; dorsal body scales heterogeneous in size and shape, all keeled, tip pointing backwards; axillary scales much smaller than remaining dorsals; enlarged dorsal scales roughly forming four or five longitudinal rows from neck to pelvis on each side of body. Nuchal and dorsal crests feebly developed, slightly raised compared to dorsals, not erect; no skin fold under nuchal and dorsal crests; MD 40. Dorsal limb scales strongly keeled, homogeneous; F4S 15/16, T4S 21/20. Ventral body scales approximately parallel, almost homogeneous, all strongly keeled, VN 61. Ventral limb scales parallel, almost homogeneous, approximately equal in size to ventrals, all strongly keeled. Tail scales all strongly keeled, ventral tail scales slightly larger than dorsal tail scales.
Dorsal surface of head grey. Two distinct black transverse bands present between orbits on dorsal surface of head and two indistinct greyish-black transverse bands present on dorsal surface of snout. Lateral surfaces of head greyish-white. Ten black radial stripes around eye on each side. Upper lips light orange. Oral cavity, inner lips and tongue pink.
Dorsal surface of body greyish-black. A light yellowish-white dorsolateral longitudinal stripe with strongly jagged upper edge and relatively straight lower edge present on each side of body from occipital region to pelvis. Some indistinct dark and light transverse bands present between two dorsolateral stripes. Some white spots scattered below dorsolateral stripe on each side of body. Dorsal surfaces of limbs dark grey. Some irregular, greyish-white transverse bands present on dorsal surfaces of limbs. Dorsal surface of tail grey with some very indistinct dark transverse bands.
Ventral surface of head white with distinct black vermiculate stripes. A little yellowish colouration present on centre of gular pouch. Ventral surfaces of body, limbs and tail white with no patterns.
The variations of morphological character of the type series are provided in Table
Morphological data of the type series of Diploderma shuoquense sp. nov. Morphometric measurements are in mm. For measurement methods and abbreviations, see the Materials and methods section.
KIZ2022004 Holotype ♂ | KIZ2022005 Paratype ♂ | KIZ2022006 Paratype ♂ | KIZ2022007 Paratype ♂ | |
---|---|---|---|---|
SVL | 52.3 | 48.2 | 48.4 | 50.5 |
TAL | 98.3 | 90.3 | 95.4 | 96.3 |
HL | 16.7 | 14.3 | 15.4 | 15.7 |
HW | 12.4 | 10.5 | 11.1 | 11.3 |
HD | 10.2 | 8.7 | 9.2 | 9.8 |
SEL | 5.7 | 5.4 | 5.4 | 5.6 |
FLL | 23.4 | 22.6 | 23.5 | 23.7 |
HLL | 36.6 | 33.4 | 36.0 | 36.4 |
T4L | 8.6 | 7.8 | 8.7 | 8.6 |
TRL | 23.6 | 22.1 | 22.5 | 21.3 |
TAL/SVL | 1.88 | 1.87 | 1.97 | 1.91 |
SEL/HL | 0.34 | 0.38 | 0.35 | 0.36 |
HW/HL | 0.74 | 0.73 | 0.72 | 0.72 |
HD/HW | 0.82 | 0.83 | 0.83 | 0.87 |
FLL/SVL | 0.45 | 0.47 | 0.49 | 0.47 |
HLL/SVL | 0.70 | 0.69 | 0.74 | 0.72 |
TRL/SVL | 0.45 | 0.46 | 0.46 | 0.42 |
SL | 10/10 | 10/10 | 9/9 | 9/8 |
IL | 9/9 | 11/11 | 10/10 | 10/11 |
NSL | 1/1 | 1/1 | 1/1 | 1/1 |
MD | 40 | 34 | 39 | 34 |
F4S | 15/16 | 14/13 | 15/15 | 15/14 |
T4S | 21/20 | 20/19 | 20/20 | 20/19 |
SOR | 3/4 | 4/4 | 3/4 | 4/4 |
VN | 61 | 59 | 57 | 56 |
From species of Diploderma which are only distributed on East Asian Islands, Diploderma shuoquense sp. nov. differs from D. brevipes, D. luei, D. makii, D. polygonatum and D. swinhonis by the presence of a transverse gular fold (vs. absence).
From species of Diploderma which are distributed on mainland, but relatively distant from that of Diploderma shuoquense sp. nov., Diploderma shuoquense sp. nov. differs from D. chapaense, D. fasciatum, D. hamptoni, D. menghaiense, D. micangshanense, D. ngoclinense and D. yunnanense by the presence of a transverse gular fold (vs. absence); from D. dymondi, D. varcoae, by having concealed tympana (vs. exposed); from D. grahami by having a much longer tail (TAL/SVL 1.87–1.97 vs. 1.64) and a distinct transverse gular fold (vs. feeble); and from D. splendidum by having jagged dorsolateral stripes in males (vs. smooth).
From species of Diploderma which occupy distributions relatively close to that of Diploderma shuoquense sp. nov. in the Hengduan Mountain Region, Diploderma shuoquense sp. nov. differs from D. panlong, D. slowinskii and D. swild by having concealed tympana (vs. exposed); from D. angustelinea, D. aorun, D. batangense, D. flavilabre, D. formosgulae, D. iadinum, D. laeviventre, D. yangi, D. yulongense and D. zhaoermii by the absence of a distinct gular spot in males in life (vs. presence of a distinct colourful gular spot); from D. brevicauda by having a relatively longer tail in males (TAL/SVL 1.87–1.97 vs. 1.40–1.84) and pink inner lips and tongue in life (vs. inner lips light yellow and tongue light orange); from D. drukdaypo by having strongly keeled ventral scales of body (vs. smooth or weakly keeled); from D. flaviceps by the presence of distinct radial stripes around the eyes (vs. absence) and the absence of a skin fold under dorsal crest in males in life (vs. presence); from D. panchi by having less mid-dorsal crest scales (MD 34–40 vs. 42–46) and smooth or weakly keeled ventral scales of head (vs. distinctively keeled); from D. qilin by having a relatively shorter tail in males (TAL/SVL 1.87–1.97 vs. 2.01–2.18); and from D. vela by having feebly developed crests without strongly erected crest scales or skin fold in males in life (vs. distinctively erected crest scales on continuous, well-developed skin fold).
Diploderma shuoquense sp. nov. is phylogenetically sister to D. bowoense, but Diploderma shuoquense sp. nov. can be differentiated from the latter by the absence of a light chrome orange gular spot in males in life (vs. presence) and having a wider head (HW/HL 0.72–0.74 vs. 0.65–0.71) and smooth or weakly keeled ventral scales of head (vs. distinctively keeled).
Diploderma shuoquense sp. nov. differs from Diploderma limingense sp. nov. by having a smaller body size in males (SVL 48.2–52.3 mm vs. 55.6–56.8 mm), vermiculate stripes covering the whole ventral head (vs. stripes not reaching the centre of gular pouch), white ventral surfaces of body, limbs and tail in males in life (vs. light brick red), pink inner lips and tongue in life (vs. inner lips bright yellow, tongue light orange) and more radial stripes around the eyes (8–10 vs. five on each side).
This species is known only from the type locality, Qingde Town, Xiangcheng County, Ganzi Prefecture, Sichuan Province, China (Fig.
This species is terrestrial, inhabiting the hot-dry valley. There are many thorny shrubs and some rock piles at the type locality (Fig.
KIZ2022009, adult male, collected on 24 April 2022 by Shuo Liu from the Jinsha River Valley, Songping Township, Yongsheng County, Lijiang City, Yunnan Province, China (27°2′2″N, 100°28′16″E, 1700 m elevation).
KIZ2022008, KIZ2022010–KIZ2022011, three adult males, collecting information the same as the holotype.
The specific epithet refers to Yongsheng County, where the new species was discovered.
Diploderma yongshengense sp. nov. can be diagnosed from congeners by a combination of the following morphological characteristics: (1) body size moderate, SVL 56.5–58.5 mm in males; (2) tail long, TAL/SVL 2.02–2.20 in males; (3) limbs relatively long, FLL/SVL 0.48–0.51 in males, HLL/SVL 0.79–0.87 in males; (4) head moderately wide, HW/HL 0.66–0.75 in males; (5) MD 38–41; (6) F4S 16–19, T4S 22–25; (7) tympanum concealed; (8) nuchal and dorsal crests moderately developed on weak skin folds in males; (9) distinct transverse gular fold present; (10) ventral scales of head and body strongly keeled; (11) ventral head scales heterogeneous in size; (12) gular spot present in males, blue or green in life; (13) dorsolateral stripes jagged in males, light yellow in life; (14) radial stripes around the eyes indistinct; (15) oral cavity, inner lips and tongue light flesh colour in life.
Adult male, SVL 58.5 mm; tail long, TAL 128.7 mm, TAL/SVL 2.20; limbs relatively long, FLL 27.9 mm on left side, FLL/SVL 0.48, HLL 46.5 mm on left, HLL/SVL 0.79. Head relatively robust, HW/HL 0.75, HD/HW 0.87; snout moderately long, SEL/HL 0.37. Rostral elongated, bordered by five small postrostral scales; dorsal head scales heterogeneous, all strongly keeled; distinct Y-shaped ridge on dorsal snout. Nasal oval, separated from first supralabial by single row of scales; loreals small, keeled; suborbital scale rows 4/4, keeled; canthus rostralis elongated, greatly overlapping with each other; enlarged, keeled scales forming single lateral ridge from posteroinferior eye to posterosuperior tympanum on each side; tympanum concealed under scales; SL 9/9, feebly keeled. Mental pentagonal; IL 11/10; enlarged chin shields 5/5, smooth, first one contacting IL on each side, remaining ones separated from IL by two rows of small scales; ventral head scales heterogeneous in size with the ones on the centre of gular pouch largest, all strongly keeled; distinct transverse gular fold present; gular pouch well developed.
Distinct shoulder fold present; dorsal body scales heterogeneous in size and shape, all keeled, tip pointing backwards; axillary scales much smaller than remaining dorsals; enlarged dorsal scales irregularly scattered on lateral surface of body. Nuchal crest scales approximately same in size and shape as dorsal crest scales; moderately developed skin fold under nuchal crest and feeble skin fold under dorsal crest; MD 38. Dorsal limb scales strongly keeled, mostly homogeneous, except a few enlarged, conical scales on postaxial thighs; F4S 17/16, T4S 23/23. Ventral body scales approximately parallel, almost homogeneous, all strongly keeled, VN 59. Ventral limb scales parallel, almost homogeneous, approximately equal in size to ventrals, all strongly keeled. Tail scales all strongly keeled, ventral tail scales larger than dorsal tail scales.
Dorsal surface of head brown with no transverse bands. Lateral surfaces of head brownish-white. No radial stripes present around eyes, only two brownish-black stripes present behind eye on each side. Oral cavity, inner lips and tongue light flesh colour.
Dorsal surface of body brown. A light yellow dorsolateral longitudinal stripe with relatively straight upper edge and strongly jagged lower edge present on each side of body from occipital region to pelvis. Some brownish-black transverse bands present between two dorsolateral stripes. Some light yellow spots scattered below dorsolateral stripe on each side of body. Dorsal surfaces of limbs greyish-brown. Some indistinct dark transverse bands present on dorsal surfaces of limbs. Dorsal surface of tail brownish-grey with some indistinct dark transverse bands.
Ventral surface of head yellowish-white. A triangular, light yellow edged light blue gular spot present on posterior central part, indistinct brown stripes present on other region of ventral head. Ventral surfaces of body, limbs and tail white with no patterns.
The variations of morphological character of the type series are provided in Table
Morphological data of the type series of Diploderma yongshengense sp. nov. Morphometric measurements are in mm. For measurement methods and abbreviations, see the Materials and methods section.
KIZ2022008 Paratype ♂ | KIZ2022009 Holotype ♂ | KIZ2022010 Paratype ♂ | KIZ2022011 Paratype ♂ | |
---|---|---|---|---|
SVL | 56.5 | 58.5 | 56.7 | 57.6 |
TAL | 117.2 | 128.7 | 114.5 | 123.0 |
HL | 17.9 | 18.7 | 17.0 | 18.8 |
HW | 12.8 | 14.1 | 12.1 | 12.5 |
HD | 11.1 | 11.0 | 10.6 | 11.3 |
SEL | 6.6 | 6.9 | 6.3 | 6.9 |
FLL | 28.6 | 27.9 | 27.8 | 27.4 |
HLL | 49.1 | 46.5 | 45.5 | 47.9 |
T4L | 12.6 | 11.8 | 11.3 | 13.1 |
TRL | 24.3 | 27.0 | 24.9 | 26.1 |
TAL/SVL | 2.07 | 2.20 | 2.02 | 2.14 |
SEL/HL | 0.37 | 0.37 | 0.37 | 0.37 |
HW/HL | 0.72 | 0.75 | 0.71 | 0.66 |
HD/HW | 0.87 | 0.78 | 0.88 | 0.90 |
FLL/SVL | 0.51 | 0.48 | 0.49 | 0.48 |
HLL/SVL | 0.87 | 0.79 | 0.80 | 0.83 |
TRL/SVL | 0.43 | 0.46 | 0.44 | 0.45 |
SL | 10/10 | 9/9 | 8/8 | 9/9 |
IL | 11/10 | 11/10 | 10/12 | 10/10 |
NSL | 1/1 | 1/1 | 1/1 | 1/1 |
MD | 41 | 38 | 41 | 39 |
F4S | 17/18 | 17/16 | 19/18 | 16/17 |
T4S | 22/23 | 23/23 | 25/24 | 24/24 |
SOR | 4/4 | 4/4 | 4/4 | 4/4 |
VN | 55 | 59 | 58 | 54 |
From species of Diploderma which are only distributed on East Asian Islands, Diploderma yongshengense sp. nov. differs from D. brevipes, D. luei, D. makii, D. polygonatum and D. swinhonis by the presence of a transverse gular fold (vs. absence).
From species of Diploderma which are distributed on mainland, but relatively distant from that of Diploderma yongshengense sp. nov., Diploderma yongshengense sp. nov. differs from D. chapaense, D. fasciatum, D. hamptoni, D. menghaiense, D. micangshanense, D. ngoclinense and D. yunnanense by the presence of a transverse gular fold (vs. absence); from D. dymondi, D. varcoae, by having concealed tympana (vs. exposed); from D. grahami by having a much longer tail (TAL/SVL 2.02–2.20 vs. 1.64) and a distinct transverse gular fold (vs. feeble); and from D. splendidum by having jagged dorsolateral stripes in males (vs. smooth).
From species of Diploderma which occupy distributions relatively close to that of Diploderma yongshengense sp. nov. in the Hengduan Mountain Region, Diploderma yongshengense sp. nov. differs from D. panlong, D. slowinskii and D. swild by having concealed tympana (vs. exposed); from D. drukdaypo and D. vela by the presence of a colourful gular spot in males in life (vs. absence); from D. angustelinea, D. bowoense, D. brevicauda, D. formosgulae, D. laeviventre, D. qilin and D. zhaoermii by having a blue or green gular spot in males in life (vs. chartreuse, lilac, orange or yellow); from D. aorun by having less distinct radial stripes around the eyes (vs. more distinct), less distinct stripes on the ventral surface of head (vs. more distinct speckles or vermiculated patterns) and heterogeneous ventral head scales (vs. homogeneous); from D. batangense by having white ventral surface of body in males in life (vs. yellow); from D. flaviceps by the presence of a colourful gular spot in males in life (vs. absence); from D. flavilabre by having light flesh coloured inner lips in life (vs. yellow); from D. iadinum by having brown dorsal ground colouration in males in life (vs. emerald green); from D. panchi by having less mid-dorsal crest scales (MD 38–41 vs. 42–46) and heterogeneous ventral head scales (vs. homogeneous); and from D. yangi by having jagged dorsolateral stripes in males (vs. smooth).
Diploderma yongshengense sp. nov. is phylogenetically sister to D. yulongense, but Diploderma yongshengense sp. nov. can be differentiated from the latter by having a blue or green gular spot in males in life (vs. chartreuse or opaline green), more distinct stripes on the ventral surface of head (vs. less distinct), white ventral and ventrolateral surface of body in males in life (vs. green) and light yellow dorsolateral stripes and enlarged scales on each side of body in males in life (vs. greenish-yellow).
Diploderma yongshengense sp. nov. differs from Diploderma limingense sp. nov. by having less mid-dorsal crest scales (MD 38–41 vs. 45–48), a blue or green gular spot in males in life (vs. yellowish-white), white ventral surfaces of body, limbs and tail in males in life (vs. light brick red) and light flesh coloured inner lips and tongue in life (vs. inner lips bright yellow, tongue light orange).
Diploderma yongshengense sp. nov. differs from Diploderma shuoquense sp. nov. by having a larger body size in males (SVL 56.5–58.5 vs. 48.2–52.3), a relatively longer tail in males (TAL/SVL 2.02–2.20 vs. 1.87–1.97), relatively longer hind limbs in males (HLL/SVL 0.79–0.87 vs. 0.69–0.74), more subdigital lamellae of fourth toe (22–25 vs. 19–21) and strongly keeled ventral scales of head (vs. smooth or weakly keeled) and the presence of a distinct colourful gular spot in males in life (vs. absence).
This species is presently known from Yongsheng and Ninglang counties, Lijiang City, Yunnan Province, China, it probably occurs in adjacent Muli County, Sichuan Province, China (Fig.
This species is terrestrial, inhabiting the hot-dry valley. There are a few trees and many rocks at the type locality (Fig.
Habitats of the new species A distant view of the type locality of Diploderma limingense sp. nov. B close view of the type locality of Diploderma limingense sp. nov. C distant view of the type locality of Diploderma shuoquense sp. nov. D close view of the type locality of Diploderma shuoquense sp. nov. E distant view of the type locality of Diploderma yongshengense sp. nov. F close view of the type locality of Diploderma yongshengense sp. nov.
Species of Diploderma can be roughly divided into two ecotypes, one inhabiting mountain forests (i.e. D. brevicauda, D. chapaense, D. dymondi, D. fasciatum, D. menghaiense, D. swild, D. varcoae, D. yunnanense and Diploderma limingense sp. nov., etc) and the other inhabiting hot-dry river valleys (i.e. D. aorun, D. bowoense, D. drukdaypo, D. formosgulae, D. laeviventre, D. vela, D. yangi, Diploderma shuoquense sp. nov. and Diploderma yongshengense sp. nov. etc). Mountain forest is often distributed in large areas. Unless there are very high mountains or very large rivers through the forest, different populations living in the forest will not be completely separated and there can be gene exchange between them. Therefore, the species inhabiting forests are usually widely distributed and their diversity is usually low. However, in the Hengduan Mountain Region, there are high mountains between the numerous river valleys, in addition, the altitude drop in different sections of the same river is usually large. Different populations living in the valleys are usually separated from each other and it is difficult for them to make gene exchange. Therefore, in contrast to the species inhabiting forests, the species inhabiting river valleys usually have very small distribution ranges and their diversity is usually very high.
Large areas of forest are not easy to be destroyed completely. Even if some parts are destroyed, there will still be many spaces for species to survive. Therefore, the species inhabiting forests are relatively less threatened by humans. On the contrary, if a section of a river valley is destroyed, such as by expansion of townships and agricultural lands, construction of tourist sites, development of highways and construction of hydroelectric plants (
Thanks to the curator of Kunming Natural History Museum of Zoology, Kunming Institute of Zoology, Chinese Academy of Sciences, for his support of the field survey and this study. We also thank the editors and reviewers for their comments on the manuscript. This work was supported by Science-Technology Basic Condition Platform from the Ministry of Science and Technology of the People’s Republic of China (2005DKA21402); state theme of Zoological Institute, Russian Academy of Sciences (122031100264-8); the funds from National Science Foundation of China (NSFC) to DQ Rao (NSFC-39570090 and NSFC-31970404); the project of Ministry of Ecology and Environment of China: Investigation and evaluation of amphibian and reptile diversity in Yunling Mountains (2019HB2096001006); and national important research and development project: Biodiversity Conservation and Restoration Technology in High Mountain and Valley Regions of south-western China (2017YFC0505202).
Uncorrected genetic pairwise distances (p-distances) (%) between species based on the mitochondrial ND2 gene sequences
Data type: Xls file.