Research Article |
Corresponding author: Javier Lobón-Rovira ( j.lobon.rovira@hotmail.com ) Academic editor: Johannes Penner
© 2022 Javier Lobón-Rovira, Werner Conradie, Ninda L. Baptista, Pedro Vaz Pinto.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Lobón-Rovira J, Conradie W, Baptista NL, Vaz Pinto P (2022) A new species of feather-tailed leaf-toed gecko, Kolekanos Heinicke, Daza, Greenbaum, Jackman, Bauer, 2014 (Squamata, Gekkonidae) from the poorly explored savannah of western Angola. ZooKeys 1127: 91-116. https://doi.org/10.3897/zookeys.1127.84942
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We here describe a new species of feather-tailed leaf-toed gecko, Kolekanos, from southern Benguela Province, Angola, based on morphological and osteological evidence, supported by phylogenetic analysis of mitochondrial data. The new species adds to the rapidly growing and newly-recognised endemic biodiversity of Angola, doubling the number of Kolekanos species, breaking the pattern observed within other closely-related African members of a clade of circum-Indian Ocean leaf-toed geckos – Ramigekko, Cryptactites and Afrogecko – all of which are presently monotypic. The new species is easily distinguished from K. plumicaudus, based on spine-like (as opposed to feather-like) scales on the margins of the original tail. Phylogenetic analyses also recovered the new taxon as monophyletic, with a well-supported sister relationship to K. plumicaudus, from which it differs by a substantial 24.1% NADH-dehydrogenase subunit 2 mitochondrial gene uncorrected p-distance.
Biodiversity, ct-scan, herpetology, osteology, Reptilia, taxonomy
African leaf-toed geckos are among the most ancient and taxonomically problematic Gekkonidae groups in Africa (
Circum-Indian Ocean leaf-toed geckos, understood as a monophyletic group that attained their current geographic distributions to reflect the landmasses distributed around the Indian Ocean during the Eocene (~40 mya) (
This new paradigm for circum-Indian leaf-toed geckos has only been addressed thanks to the rapid growth of new molecular techniques in the last two decades and the intensive surveys in previously poorly or unexplored regions in Africa, like Angola (
Access to newly-collected material from these regions has brought new opportunities to understand the evolutionary patterns of African herpetofauna, especially African gekkonids. This is particularly noteworthy in terms of the remarkable increase in knowledge of Angolan herpetofauna, with the description of 34 new species (
Angolan leaf-toed geckos had previously been considered as members of Afrogecko Bauer, Good & Branch, 1997, represented by two species, A. plumicaudus Haacke, 2008 and A. ansorgii (Boulenger 1902). Until recent studies, both species were poorly known and with very restricted geographical distribution in south-western Angola (
Scientific studies have been increasing in Angola in recent years, following a long civil war that prevented fieldwork in this region of Africa for several decades until the early 2000s (
Kolekanos specimens and tissue samples have been collected from Namibe Province, Angola, since 2009 (
A mitochondrial gene NADH-dehydrogenase subunit 2 (ND2, 1041 bp) was used, comprising information from nine individuals of Kolekanos from the new northern records, to generate data for phylogenetic analysis to explore phylogenetic relationships amongst Kolekanos (Table
Detailed collection and observational records of Kolekanos spp., including information on species, catalogue numbers, field numbers, localities, geographical coordinates and source of records. Abbreviations: California Academy of Science (
Species | Catalog Number | Field Number | Locality | GPS Coordinates | Source |
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Kolekanos plumicaudus |
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– | Tambor | -16.1355, 12.4297 |
|
Kolekanos plumicaudus |
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– | Curoca River Crossing | -16.3027, 12.4165 |
|
Kolekanos plumicaudus |
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– | 11 km NE from Iona | -16.8606, 12.6106 |
|
Kolekanos plumicaudus |
|
– | 7 km NE from Iona | -16.8583, 12.6127 |
|
Kolekanos plumicaudus | FKH 0235 | P9.254 | Camp Baptista Cunene | -17.1603, 12.0182 |
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Kolekanos plumicaudus | FKH 0236 | P9.255 | Camp Baptista Cunene | -17.1603, 12.0182 |
|
Kolekanos plumicaudus |
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– | Omauha | -16.1996, 12.3987 |
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Kolekanos plumicaudus | FKH-0782 | JLRZC0109 | Omauha | -16.1987, 12,401258 |
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Kolekanos plumicaudus | FKH-0343 | P9.286 | Omauha | -16.1996, 12.3987 |
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Kolekanos plumicaudus | FKH-0344 | P9.287 | Omauha | -16.1996, 12.3987 |
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Kolekanos plumicaudus | FKH-0345 | P9.288 | Omauha | -16.1996, 12.3987 |
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Kolekanos plumicaudus | FKH-0346 | P9.289 | Omauha | -16.1996, 12.3987 |
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Kolekanos plumicaudus | NA | NA | Mutuovano | -15.9153, 12.3848 |
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Kolekanos plumicaudus | NA | NA | Muende-Curoca | -16.2892, 12.3180 |
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Kolekanos plumicaudus | NA | NA | Tchitchaki | -16.2877, 12.2753 |
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Kolekanos plumicaudus | NA | NA | Humbi | -16.9858, 12.5415 |
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Kolekanos plumicaudus | NA | NA | Congundo | -17.0396, 12.6013 |
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Kolekanos plumicaudus | NA | NA | Conguiungulo | -16.8437, 12.6141 |
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Kolekanos plumicaudus | FKH-0534 | P1.021 | Maongo-Giraul | -15.0326, 12.4146 |
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Kolekanos plumicaudus | FKH-0535 | P1.022 | Maongo-Giraul | -15.0326, 12.4146 |
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Kolekanos plumicaudus | – | P1.075 | Chamaleva | -15.6863, 12.6124 |
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Kolekanos plumicaudus |
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– | Tchamalindi | -16.9752, 12.8833 |
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Kolekanos plumicaudus |
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– | Tchamalindi | -16.9752, 12.8833 |
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Kolekanos plumicaudus | – | P1.126 | Cafema | -17.1289, 12.5138 |
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Kolekanos plumicaudus | – | P1.127 | Cafema | -17.1306, 12.5067 |
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Kolekanos plumicaudus | FKH-0574 | P1.115 | Tchamalinde | -16.9752, 12.8833 | This work |
Kolekanos plumicaudus | FKH-0661 | P1.246 | Maongo | -15.0461, 12.4310 | This work |
Kolekanos plumicaudus | FKH-0662 | P1.247 | Maongo | -15.0461, 12.4310 | This work |
Kolekanos plumicaudus | FKH-0663 | P1.248 | Maongo | -15.0461, 12.4310 | This work |
Kolekanos spinicaudus sp. nov. | FKH-0645 | P1.227 | Carivo | -13.1923, 13.4211 | This work |
Kolekanos spinicaudus sp. nov. |
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P1.228 | Carivo | -13.1923, 13.4211 | This work |
Kolekanos spinicaudus sp. nov. | FKH-0647 | P1.229 | Carivo | -13.1923, 13.4211 | This work |
Kolekanos spinicaudus sp. nov. | FKH-0648 | P1.230 | Carivo | -13.1923, 13.4211 | This work |
Kolekanos spinicaudus sp. nov. | FKH-0649 | P1.231 | Carivo | -13.1923, 13.4211 | This work |
Kolekanos spinicaudus sp. nov. | FKH-0650 | P1.232 | Carivo | -13.1923, 13.4211 | This work |
Kolekanos spinicaudus sp. nov. |
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JLRZC0212 | Ekongo | -13.2494, 13.2065 | This work |
Kolekanos spinicaudus sp. nov. | FKH-0845 | JLRZC0213 | Ekongo | -13.2494, 13.2065 | This work |
Kolekanos spinicaudus sp. nov. |
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JLRZC0214 | Ekongo | -13.2494, 13.2065 | This work |
To determine the correct placement of the species and explore diversification within Kolekanos plumicaudus, Bayesian Inference (BI) and Maximum Likelihood (ML) analyses were performed using the ND2 sequence alignment. The best partition scheme and best-fitting models of molecular evolution were selected using PartitionFinder v.1.1.1 (
Finally, uncorrected pairwise sequence divergences (p-distance) were calculated for the ND2 sequences, in MEGA v.10.1.7 (
For this study, we examined 19 adult specimens of Kolekanos, collected during different expeditions and deposited in the National Museum of Natural Science (
For osteological comparisons, we performed High Resolution X-ray Computed Tomography (HRCT) scans of one adult female (
While the two phylogenetic analyses (BI and ML) did not retrieve the same topology with regard to the deeper-level topological structuring, both were largely concordant in recovering the monophyletic circum-Indian Ocean group and recognising two clearly distinct sister taxa within Kolekanos (Fig.
Maximum Likelihood phylogeny, with Bayesian Inference support overlaid. Support values (ML BS = Maximum Likelihood bootstrap values; BI PP = Bayesian Inference posterior probabilities) are shown graphically at the nodes according to the colours shown in the inset key. Kolekanos spinicaudus sp. nov. is highlighted in red.
ND2 divergences (uncorrected pairwise distances) between circum-Indian leaf-toed geckos. Bold values depict intraspecific divergences.
ID | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
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1. Afrogecko porphyreus | 16.28 | |||||||||
2. Ramigekko swartbergensis | 28.11 | 2.55 | ||||||||
3. Kolekanos plumicaudus | 30.83 | 32.48 | 3.79 | |||||||
4. Kolekanos spinicaudus sp. nov. | 31.33 | 31.43 | 24.49 | 7.26 | ||||||
5. Cryptactites peringueyi | 28.27 | 23.06 | 32.83 | 31.51 | 0.58 | |||||
6. Matoatoa breviceps | 26.50 | 28.07 | 29.95 | 30.01 | 28.75 | 0 | ||||
7. Christinus alexanderi | 24.50 | 26.32 | 29.92 | 31.75 | 26.42 | 23.42 | 0 | |||
8. Christinus marmoratus | 25.32 | 27.08 | 29.40 | 30.80 | 27.20 | 25.31 | 13.52 | 7.95 | ||
9. Christinus guentheri | 24.29 | 26.47 | 29.06 | 29.13 | 26.84 | 23.49 | 13.52 | 15.03 | 0.32 | |
10. Goggia lineata | 31.11 | 32.42 | 34.74 | 35.29 | 32.15 | 30.70 | 28.80 | 30.19 | 29.56 | n/c |
Morphological analysis revealed morphological differences between the two main clades within Kolekanos. PCA analysis explained a considerable part of the variation within these clades, with PC1 (46.9% of variation) and PC2 (15.6% of variation) showing two well-separated groups (Fig.
A PCA plots of the first principal component (PC 1) versus the second (PC 2) of morphometric analysis for the two species of Kolekanos. The green polygon denotes the distribution within PCAs of K. spinicaudus sp. nov. and the pink polygon of K. plumicaudus. For loadings of all axis and explained variance, see Suppl. material
Although the osteological reconstruction demonstrated the skulls of Kolekanos to be very conserved, we did find differences, mostly in overall shape of the head, supporting the above morphological findings (Fig.
Detailed views in A dorsal B frontal C lateral D posterior and E ventral of skull and F lateral, dorsal, medial and ventral of left jaw (from top to bottom) of K. spinicaudus sp. nov. (MNCN50769). Detailed views in G dorsal H frontal I lateral G posterior and K ventral view of skull and L lateral, dorsal, medial and ventral of left jaw (from top to bottom) of Kolekanos plumicaudus (MNCN50770). Abbreviations: Bc, braincase; Co, coronoid; CB, compound bone; D, dentary; EcP, ectopterygoid; EP, epipterygoid; F, frontal; J, jugal; M, maxilla; N, nasal; Ot, otostapes; P, parietal; PF, prefrontal; Pl, palatine; PM, premaxilla; PO, postorbitofrontal; Pt, pterygoid; Q, quadrate; Sp, splenial; SR, sclerotic ring; V, vomer.
Therefore, the above morphological and phylogenetic differences support the recognition of two different species within Kolekanos and we take the opportunity to describe the second lineage recovered as a new species below. In this manuscript, we have applied the general lineage-based species concept, where we treat all independent evolving lineages represented and supported by multiple lines of evidence, as listed above, as separate species (
The name “spinicaudus” is derived from the combination of the Latin words “spina” and “cauda”, that refers to the spiny appearance of the tail of the new species. The species epithet is used as a singular nominative adjective “-us”.
Kolekanos can be easily differentiated from other circum-Indian leaf-toed and African leaf-toed geckos, based on its ornamented tail (versus non-ornamented tail in the remaining genera). The new species differs from K. plumicaudus, based on the following characters: different ornamentation of the tail, being composed by modified scales on the margins of the original tail which resemble white lateral spines (versus feathered-like tail in K. plumicaudus); broader head (minimum HW = 7.95 mm versus maximum HW = 7.35 mm in K. plumicaudus); more robust body, with shorter forelimbs (versus thinner and more slender body in K. plumicaudus, Fig.
(Fig.
Morphological (morphometric and meristic) of Kolekanos spinicaudus sp. nov. Measurements are represented in millimetres (mm). For abbreviations, see Material and methods section. R = regenerated tail, M = male, F = female.
Species | K. spinicaudus sp. nov. | K. spinicaudus sp. nov. | K. spinicaudus sp. nov. | K. spinicaudus sp. nov. | K. spinicaudus sp. nov. | K. spinicaudus sp. nov. | K. spinicaudus sp. nov. | K. spinicaudus sp. nov. | K. spinicaudus sp. nov. |
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Catalogue# |
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FKH0845 |
|
|
FKH-0645 | FKH-0648 | FKH-0647 | FKH-0650 |
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Status | Holotype | Paratype | Paratype | Paratype | Paratype | Paratype | Paratype | Paratype | Paratype |
Sex | M | F | F | M | F | M | M | F | M |
SVL | 44.59 | 43.19 | 44.15 | 40.82 | 43.71 | 41.66 | 42.72 | 41.65 | 35.87 |
TAL | R 36.77 | – | R 22.26 | R 39.76 | R 31.39 | 36.85 | R 28.85 | – | 39.44 |
TrunkL (mm) | 19.82 | 17.87 | 18.65 | 16.85 | 19.36 | 18.44 | 19.80 | 18.27 | 16.71 |
HL (mm) | 10.99 | 11.67 | 11.6 | 11.09 | 11.10 | 10.76 | 11.06 | 10.88 | 9.59 |
HW (mm) | 8.63 | 8.12 | 8.56 | 8.48 | 8.28 | 7.95 | 8.21 | 8.26 | 7.18 |
HH (mm) | 2.95 | 4.20 | 3.80 | 4.00 | 3.52 | 3.77 | 3.86 | 3.60 | 3.17 |
OD | 2.35 | 2.35 | 2.50 | 2.51 | 2.62 | 2.35 | 2.62 | 2.21 | 2.08 |
EL | 0.47 | 0.65 | 0.85 | 0.69 | 0.76 | 0.64 | 0.48 | 0.47 | 0.54 |
CL | 8.03 | 8.59 | 9.01 | 8.74 | 8.15 | 8.01 | 7.80 | 8.15 | 7.94 |
FL | 10.39 | 10,.68 | 11.10 | 9.94 | 10.93 | 10.73 | 10.13 | 10.40 | 8.49 |
NE | 3.30 | 3.40 | 3.75 | 3.44 | 3.61 | 3.35 | 3.55 | 3.43 | 2.99 |
SE | 4.48 | 4.50 | 4.95 | 4.68 | 4.56 | 4.31 | 4.67 | 4.46 | 3.72 |
EE | 3.72 | 3.21 | 3.65 | 3.76 | 3.66 | 3.31 | 3.57 | 3.10 | 3.12 |
IN | 1.55 | 1.55 | 1.48 | 1.45 | 1.45 | 1.40 | 1.34 | 1.39 | 1.22 |
IO | 4.29 | 4.33 | 4.22 | 4.54 | 4.33 | 3.45 | 4.19 | 4.21 | 3.74 |
N° lamellae 1st toe (Right/Left) | 4/3 | 5/5 | 4/5 | 4/5 | 4/4 | 4/4 | 4/3 | 4/3 | 5/4 |
N° lamellae 4th toe (Right/Left) | 5/5 | 7/5 | 6/6 | 6/5 | 6/7 | 5/5 | 6/5 | 6/6 | 6/6 |
N° lamellae 1st finger (Right/Left) | 3/4 | 4/ | 4/4 | 3/3 | 4/4 | 5/4 | 4/3 | 3/4 | 3/3 |
N° lamellae 4th finger (Right/Left) | 6/7 | 5/6 | 6/7 | 6/6 | 5/6 | 5/6 | 6/4 | 5/7 | 7/6 |
N° postmental | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
N° infralabial | 9 | 8 | 8 | 9 | 9 | 9 | 9 | 9 | 9 |
N° supralabial | 8 | 10 | 10 | 10 | 9 | 9 | 10 | 9 | 9 |
N° internasal | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 |
N° scales ear to eye | 17 | 18 | 16 | 17 | 12 | 13 | 14 | 15 | 16 |
N° scales eye to nostril | 11 | 13 | 11 | 12 | 11 | 10 | 11 | 10 | 12 |
N° scales eye to eye | 17 | 20 | 15 | 16 | 16 | 15 | 17 | 18 | 16 |
Variation in scalation and body measurements of the paratypes of K. spinicaudus sp. nov. are reported in Table
In life
(Fig.
Holotype of Kolekanos spinicaudus sp. nov. (MNCN50769) from Carivo, Benguela Province, Angola A dorsal and ventral view of whole specimen B detail of head (from top to bottom) in dorsal, lateral and ventral views C detail of pelvic region and hind-limbs in ventral view D detail of left fingers. Photos by Alberto Sanchez Vialas (
(Fig.
(Fig.
Morphological (morphometric and meristic) of Kolekanos plumicaudus. Measurements are represented in millimetres (mm). For abbreviations, see Material and methods section, R = regenerated, M = male, F = female.
Species | K. plumicaudus | K. plumicaudus | K. plumicaudus | K. plumicaudus | K. plumicaudus | K. plumicaudus | K. plumicaudus | K. plumicaudus | K. plumicaudus | K. plumicaudus |
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Catalogue# |
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FKH-0661 | FKH-0663 |
|
|
|
|
|
|
|
Status | – | – | – | – | – | – | – | – | – | – |
Sex | F | F | F | F | M | M | F | F | F | M |
SVL | 41.91 | 38.45 | 40.22 | 42.05 | 42 | 36.57 | 41.84 | 36.97 | 41.84 | 37.38 |
TAL | R 34.14 | R 34.38 | 41.91 | 40.34 | – | R 26.35 | – | 33.34 | – | 34.68 |
TrunkL (mm) | 19.08 | 17.84 | 19.07 | 18.35 | 18.38 | 16.20 | 18.41 | 16.97 | 19.38 | 20.03 |
HL (mm) | 10.04 | 9.42 | 9.89 | 11.11 | 11.17 | 10.59 | 11.59 | 10.22 | 11.53 | 10.98 |
HW (mm) | 7.03 | 6.91 | 6.81 | 6.71 | 7.07 | 6.29 | 7.13 | 6.92 | 7.35 | 6.70 |
HH (mm) | 3.50 | 3.38 | 3.27 | 3.45 | 3.19 | 2.76 | 3.62 | 2.90 | 3.21 | 30.70 |
OD | 2.08 | 2.28 | 2.17 | 2.35 | 2.31 | 2.11 | 2.34 | 2.14 | 2.42 | 2.10 |
EL | 0.59 | 0.49 | 0.50 | 0.71 | 0.79 | 0.58 | 0.63 | 0.87 | 0.75 | 0.62 |
CL | 8.15 | 7.39 | 8.11 | 8.90 | 8.34 | 7.33 | 7.45 | 8.05 | 8.41 | 7.85 |
FL | 10.75 | 8.77 | 9.69 | 10.38 | 11.76 | 10.54 | 12.42 | 11.85 | 12.86 | 12.66 |
NE | 2.91 | 2.80 | 2.97 | 3.15 | 3.23 | 3.15 | 3.49 | 2.83 | 3.32 | 3.06 |
SE | 3.89 | 3.61 | 3.65 | 4.18 | 4.07 | 3.98 | 4.13 | 3.92 | 4.24 | 4.18 |
EE | 3.71 | 3.00 | 3.49 | 3.47 | 3.45 | 2.91 | 3.49 | 3.19 | 3.25 | 2.96 |
IN | 1.30 | 1.29 | 1.24 | 1.30 | 1.21 | 1.11 | 1.25 | 1.26 | 1.45 | 1.43 |
IO | 3.71 | 3.57 | 3.78 | 3.16 | 3.06 | 3.03 | 3.29 | 3.19 | 3.46 | 3.04 |
N° lamellae 1st toe (Right/Left) | 4/4 | 3/3 | 4/3 | 4/4 | 4/4 | 4/4 | 4/4 | 6/6 | 5/4 | 4/4 |
N° lamellae 4th toe (Right/Left) | 6/5 | 5/6 | 5/6 | 6/6 | 7/7 | 6/6 | 7/4 | 5/6 | 6/7 | 7/8 |
N° lamellae 1st finger (Right/Left) | 3/4 | 3/4 | 3/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 |
N° lamellae 4th finger (Right/Left) | d/6 | 6/6 | 6/6 | 6/6 | 8/8 | 7/7 | 8/8 | 7/7 | 7/7 | 7/7 |
N° postmental | 2 | 1 | 2 | 3 | 2 | 2 | 2 | 3 | 2 | 2 |
N° infralabial | 8 | 8 | 9 | 9 | 9 | 9 | 9 | 9 | 9 | 9 |
N° supralabial | 10 | 10 | 10 | 10 | 9 | 10 | 9 | 10 | 9 | 10 |
N° internasal | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 1 |
N° scales ear to eye | 13 | 14 | 15 | 17 | 17 | 17 | 18 | 16 | 15 | 17 |
N° scales eye to nostril | 11 | 9 | 11 | 10 | 9 | 10 | 8 | 8 | 8 | 9 |
N° scales eye to eye | 15 | 14 | 15 | 16 | 18 | 15 | 14 | 15 | 15 | 14 |
The species seems relatively common, but highly localised. Although the general habitat does not appear to be threatened, more research is needed to confirm if the species’ distribution is larger than currently known. Therefore, following the IUCN Red List guidelines (
Using molecular and morphological evidence, we herein described a new leaf-toed gecko, from southern Benguela Province, Angola, Kolekanos spinicaudus sp. nov., thereby adding another species to the growing list of gekkonids described in the last decade from this poorly-known African country (
The molecular analysis, provided in this work, has shown a large divergence of the ND2 mitochondrial gene between K. spinicaudus sp. nov. and K. plumicaudus, being even higher than the molecular divergence found between closely-related genera, such as with Rammigekko and Cryptactites. However, the external morphological similarities between these two taxa support differentiation only at species level. This high divergence can be explained by the ancient character of this group (
Regarding interspecific variation, both species have exhibited a high degree of morphological and ecological differentiation. While K. plumicaudus presented a more slender head and body and seems more strongly associated with the more arid environments of the Angolan Kaokoveld Desert in Namibe Province, the sister species, K. spinicaudus sp. nov. presented a more robust head, body and limbs and is apparently only found in the semi-arid savannahs of Benguela Province (
Some of the osteological features provided by
We here provide another example of diversification in south-western Angola, leading to speciation in the more arid desert ecosystems of Namibe Province and in the semi-arid coastal savannahs of Benguela Province, a pattern that has been found in other studies (e.g. Hemidactylus benguellensis-group,
To conclude, we recommend additional surveys in Benguela Province to study the distribution and abundance of this new species to assess its conservation status and further research is needed in northern Namibe Province to explore potential contact zones between the two Kolekanos species. Due to the high genetic divergence between the two recorded populations of K. spinicaudus sp. nov., we also suggest caution when addressing conservation strategies in western Angola, since it may affect ongoing speciation processes within Kolekanos in this region.
We thank Alvarito Eugénio, the owner of Carivo Farm, where we performed frequent surveys and where topotypical material was recovered, for his very kind hospitality. We also thank Luis Pittagrós and “Bibi” for their logistical support and precious advice. In addition, the INBC – Instituto Nacional da Biodiversidade e Conservação within the Ministry of Culture, Tourism and Environment (MCTE) and especially Director of INBC, Drª Albertina Nzuzi, for issuing research and export permits (no. 01/2021). We thank Fernanda Lages and ISCED – Instituto Superior de Ciências da Educação da Huíla for logistical support. We are grateful to Vladimir Russo from Fundação Kissama for critical administrative and logistical support. We also thank to Alberto Sanchez Vialas from
Additional genetic material used for this work with their corresponding field numbers, catalog numbers and GenBank accession numbers
Data type: Genetic material.
Explanation note: AMB – A. M. Bauer field series; AMS – Australian Museum;
Results of the analysis of morphometric differences between Kolekanos spp. and sexes
Data type: Morphological results.
Explanation note: Analyses were performed using permutational ANOVAs (for SVL, indicated as an *, and with an associated degrees of freedom of 1,14) and ANCOVAs (all the other variables, using SVL as a covariate). Significant results (p-values < 0.05) are highlighted in bold. For abbreviations see Material and methods section.
Principal Component Analysis (PCA) loadings for each morphological variable measured in of Kolekanos spp., including standard deviation (SD), percentage of variance (% Variance) and cumulative proportion for each component.
Data type: Statistics.
Explanation note: For abbreviations, see Materials and Methods section.