Species of this new genus can be distinguished from members of the remaining doryctine genera with long, petiolate first metasomal tergite (e.g. Bolivar, Notiospathius, Pecnobracon Kieffer et Jöergensen, Spathius, Trigonophasmus Enderlein) by having the mesoscutum sharply declivous anteriorly with sharp anterolateral corners. Sergey could be included in the key to dorcytine genera of the New World (Marsh 1997) as follows:

Head: not depressed. Ocelli arranged in almost equilateral triangle. Frons not distinctly excavated, without a median keel between antennal sockets. Occipital carina complete, fused with hypostomal carina before mandible. Malar suture absent. Clypeus not high, delineated from face by distinct furrow, with fine lower flange. Hypoclypeal depression wide, round. Postgenal bridge narrow. Maxillary palpi 5-segmented, apical segment longer than fourth segment; labial palpi short, 4-segmented, third segment not shortened. Scape of antenna wide and rather short, without flange apically and ventroapical lobe, without basal constriction; ventral margin of scape shorter than dorsal margin in lateral view. First flagellar segment about the same length as second segment, usually several apical or subapical segments whithish. Apical segment more or less pointed apically, without “spine”.

Mesosoma: not depressed. Neck of prothorax short but visible in dorsal view. Pronotum dorsally weakly convex (lateral view), with a transverse carina and a scrobiculate pronotal sulcus. Pronope absent. Propleural dorsoposterior flange rather short. Mesonotum distinctly elevated above pronotum. Anterolateral corners of mesoscutum projected in two flanges (Figs 1–2). Notauli present and complete, scrobiculate, obscured in rugose median area of mesoscutum. Scuto-scutellar (transscutal) suture distinct and complete. Prescutellar depression, with 3–5 high carinae. Scutellum slightly convex, subtriangular in dorsal view, about as long as wide, without lateral carinae. Subalar depression distinct. Mesopleuron with subalar sulcus, sternaulus and posterior mesopleural sulcus coarsely sculptured, otherwise smooth and polished. Mesopleural pit distinct. Precoxal sulcus (sternaulus) rather deep, wide, and scrobiculate, extended at least two thirds length of mesopleuron. Prepectal carina distinct and complete, laterally reaching anterior margin of subalar depression. Propodeum with two dorsolateral areas delimited by distinct carinae; sometimes these divergent carinae suggest an areola enclosing a rugose area (Fig. 1), in other cases the propodeum is uniformly rugose-areolate beyond the dorsolateral areas. Propodeal bridge absent. Propodeal spiracles small and round. Metapleuron slightly convex, entirely sculptured, rugose-areolate.

Figures 1–2. 

SEM images of Sergey tzeltal sp. n.; 1 head and mesosoma in dorsal view 2 head and mesosoma in lateral view. Arrows indicate anterolateral sharp edges/flanges on mesoscutum and divergent carinae on propodeum.

Wings: veins RS and r-m present, thus first and second submarginal cells entirely closed. Second submarginal cell rather long and narrow. First subdiscal cell open postero-apically, vein 2cu-a absent. Veins 1a and 2a absent. Hind wing with vein C+Sc+R longer than vein SC+R. Vein RS arising from vein R far from vein r-m. Marginal cell more or less distinctly narrowed towards apex, without vein r. Vein cu-a present. Vein M+CU about 0.6-0.7 times as long as 1M; vein m-cu straight. Male hind wing without stigma-like swelling of basal veins.

Legs: Fore tibia on inner surface with several long and slender spines arranged along its anterior margin in almost single vertical line. Hind coxa long and narrow, with basoventral tubercle. Claws simple.

Metasoma: first tergite petiolate, long and narrow, usually striate-coriaceous, with some transverse carinae basally, these carinae sometimes reduced. Basal sternal plate (acrosternite) of first tergite long, 0.6–0.7 times as long as first tergite, extended distinctly beyond level of spiracles. Dorsope of first tergite small and shallow; spiracular tubercles indistinct, situated in basal 0.3 of tergite. Second tergite without distinct furrows and areas. Second suture considerably shallow, complete, almost straight in females and distinctly curved in males. Third tergite without transverse furrow and basal area. Tergites behind second with a single transverse line of sparse long erect setae. Ovipositor distinctly darkened apically, with two distinct subapical nodes. Ovipositor sheaths long, about as long as metasoma or slightly longer.

We are very pleased to name this genus after our dear friend and colleague Dr. Sergey A. Belokobylskij, for his great contribution to the taxonomic knowledge of the braconid subfamily Doryctinae. Gender is to be considered masculine.

Sergey tzeltal sp. n.

This is the most distinctive species of the genus. It can be distinguished from the remaining members of Sergey by having the eyes considerably smaller, their height about as long as malar space (distinctly longer than malar space in the remaining species); and the first metasomal tergite broad, 1.5 times longer than its apical width (slender, at least 2.1 times longer than its apical width in the remaining species).

Body length 2.1mm (Fig. 3), fore wing 1.5mm; ovipositor sheaths 1.1mm. Colour: head excluding antennae, mesoscutum and mesopleuron brown, otherwise uniformly honey yellow. Head: about as high as wide (anterior view) (Fig. 4), 0.7 times as long as wide (dorsal view). Clypeus, face, frons and vertex largely smooth and shining (Fig. 5), with a few shallow rugae near the mandible insertion and antennal sockets; temple smooth. Eye small 1.5 times higher than wide. Malar space height/eye height ratio 1.1 (Fig. 4). Temple/eye length ratio (dorsal view) 0.6. Antenna incomplete, only with nine basal flagellomeres; first flagellomere about four times longer than wide and as long as second.

Mesosoma: 2.0 times longer than wide and 1.9 times longer than high (Fig. 6). Pronotal groove wide, and scrobiculate, pronotal carina distinct. Propleuron rugose on median third. Mesoscutum transverse, 0.6 times as long as wide. Mesoscutal lobes smooth, notauli deep and scrobiculate, obscured in an irregularly rugose median area before reaching the scuto-scutellar suture (Fig. 9). Prescutellar sulcus with three carinae, the median one straight, and the lateral ones irregular. Scutellar disc smooth and triangular. Mesopleuron smooth. Precoxal sulcus, deep, wide and scrobiculate-rugose, running along the entire length of mesopleuron. Subalar sulcus deep and rugose. Metanotum with a median carina but without a distinct projection. Metapleuron entirely areolate rugose. Propodeum with two divergent carinae running from median anterior edge delimiting two smooth dorsolateral areas, beyond these carinae it is almost uniformly areolate rugose.

Wings: fore wing (Fig. 7) length 3.0 mm, length/width ratio 3.85; vein 1cu-a postfurcal to vein 1M; veins 2RS/2M ratio 0.5.

Legs: fore tibia with a row of spines. Hind coxa slightly striate dorsally, smooth ventrally, with a small but distinct basoventral tubercule.

Metasoma: basal sternal plate/length of first tergum 0.6. First metasomal tergite 2.8 times longer than apically wide (Figs 8, 10). Second median tergite longitudinally striate on basal one fifth, smooth apically. Suture between second and third median tergites slightly sinuate. Remaining terga smooth and polished. Ovipositor length 1.2mm, 1.1 times as long as metasoma.

Male. Unknown.

Known only from a submontane forest at the type locality in Coahuila, Mexico.

Unknown.

The specific epithet refers to Coahuila, the Mexican state where the type locality of this species is located.

Holotype: female (CNIN), Mexico, Coahuila, Mpio. Torreón, Sierra de Jumillo, Arroyo Palos, 09-11/X/2009, 25.13 N - 103.27 O, 2006 msnm, DNA voucher no. IB-CNIN-637, GenBank accession nos. JN870454 (COI), JN870613 (cyt b), KC822013 (EF-1alpha; not included in this work), JN870735 (wingless, not included in this work).

This distinctive species can be distinguished from the remaining species of Sergey by having: 1) a mostly yellow body colour (brown to black in the remaining species); 2) head and mesoscutum distinctly sculptured, transversally striate (entirely smooth and polished in the remaining species); and 3) fore wing with vein m-cu reaching vein RS+M basally to 2RS, thus vein (RS+M)b present and distinct (m-cu reaching vein RS+M interstitial with respect to vein 2RS, thus vein (RS+M)b absent in the remaining species).

Body length 3.1mm (Fig. 11), fore wing 2,5 mm; ovipositor sheaths 3.5 mm. Colour: most part of the body yellow; apical third of propodeum and first metasomal tergite dark brown, second metasomal tergite yellow with lateral areas brown; antennae honey yellow, gradually darkening toward apex, subapical 20^th to 23^rd segments white (Fig. 15), apical three segments dark brown; fore and middle coxae pale yellow; fore and middle tibiae brown to dark brown; trochanter and trochantellus pale yellow; tarsi brown to dark brown; hind coxa pale yellow basally, dark brown apically; hind femur and tibia with four alternate yellow and dark brown transversal bands. Wings hyaline; pterostigma and veins brown. Ovipositor sheaths yellow to honey yellow.

Head: 0.7 times as high as wide in anterior view (Fig. 12), 0.6 times as long as wide in dorsal view (Fig. 13). Vertex and frons distinctly striate; face, temple and gena smooth (Fig. 14); clypeus transversally striate. Eye 1.2 times higher than wide. Malar space height/eye height ratio 0.3 (Fig. 12). Temple/eye length ratio (dorsal view) 0.4. Antenna with 26 flagellomeres, first flagellomere about four times longer than wide and as long as second.

Mesosoma: about 1.9 times longer than wide and 2.0 times longer than high (Figs 16–17). Pronotal groove wide, deep, and scrobiculate, pronotal carina distinct. Propleuron smooth to slightly rugose. Mesoscutum slightly transverse, 0.7 times as long as wide. Mesoscutal lobes transversally striate with coriaceous microsculpture, notauli deep, complete and scrobiculate (Fig. 17), not joining, reaching the end of mesoscutum, obscuring in an irregular longitudinal rugose median area before reaching the scuto-scutellar suture. Prescutellar sulcus with four distinct carinae. Scutellar disc smooth and triangular. Mesopleuron smooth. Precoxal sulcus, deep and scrobiculate, running along the entire length of mesopleuron. Subalar sulcus deep and scrobiculate. Metanotum with a distinct median carina-like projection. Metapleuron entirely areolate-rugose. Propodeum uniformly areolate-rugose, with two longitudinal carinae joined basally and that immediately diverge forming an areola-like structure.

Wings: fore wing length 3.6 mm, length/width ratio 3.7; vein 1cu-a slightly postfurcal to vein 1M, thus vein (RS+M)b present (Fig. 18); veins 2RS/2M ratio 0.5.

Legs: fore tibia with a row of spines. Hind coxa transversally striate-rugose, with a small but distinct basoventral tubercle..

Metasoma: Basal sternal plate/length of first tergum 0.6. First metasomal tergite 2.5 times longer than apically wide (Fig. 19). Second median tergite longitudinally costate on basal three fourths, smooth on apical fourth. Suture between second and third median tergites sinuate laterally. Remaining terga smooth and polished. Ovipositor length 3.5 mm, 1.8 times longer than metasoma.

Body length 3.4–4.3 mm. Temple/eye length ratio in dorsal view 0.4–0.5. Antenna with 26–28 flagellomeres. Prescutellar sulcus with four or five carinae. Fore wing length 3.5–3.6 mm, length/width ratio 3.7–3.8 times its maximum width. Ovipositor length 3.5–4.3 mm, 1.8-2.0 times longer than metasoma.

Males. Unknown.

Known only from the type locality in southern Cuba.

Unknown.

This species is named after the Caribbean country where it occurs, Cuba.

Holotype (CNIN): Female, Cuba, Santiago, Gran Piedra Isabélica, 06-14/VII/1995, FIT, Cloud Forest, 1100m, S.B. Peck, DNA voucher number CNIN413, GenBank accession numbers JN870310 (COI), JN870491 (cyt b), KC822012 (EF-1alpha; not included in this work), KC822095 (wingless; not included in this work). Paratype (CNIN): one female, same data as holotype; DNA voucher number CNIN414, GenBank accession numbers JN870311 (COI), JN870492 (cyt b), JN870651 (wingless; not included in this work).

This species is similar to S. tzotzil, but it can be distinguished from the latter species by the colour pattern of the white band on the female antenna. In S. tzeltal, the white band is either apical or subapical and is composed of at least two entire whitish flagellomeres, usually more, with at most three apical flagellomeres brown. In S. tzotzil, the white band is subapical and consists only of the lighter color on the articulation between the 19^th and 20^th flagellomeres, and with the five apical flagellomeres brown.

Body length 3.1 mm (Fig. 20), fore wing 2.7 mm; ovipositor sheaths 1.5mm. Colour: head uniformly brown, antenna brown, gradually darkening towards apex, except for a subapical white band composed of 2–4 flagellomeres (apical in Oaxaca population composed of 6-8 flagellomeres, see below) (Figs 24, 26). Mesosoma uniformly dark brown, except for a slightly lighter area on the median area of mesoscutum. Metasoma brown. Legs light brown, except fore and middle coxae, trochanters and trochantelli and hind trochantellus, which are pale yellow; hind coxa and apical three fourths of hind femur dark brown. Wings hyaline; pterostigma and veins brown. Ovipositor sheaths brown.

Head: 0.8 times as high as wide in anterior view (Fig. 21), and 0.7 times as long as wide in dorsal view (Fig. 22). Clypeus, face, frons and vertex smooth and polished; temple smooth. Eye 1.3 times higher than wide. Malar space height/eye height ratio 0.3. Temple/eye length ratio (dorsal view) 0.6. Antenna with 24 flagellomeres, first flagellomere about 4.0 times longer than wide, as long as second one.

Mesosoma: 2.0 times longer than wide (Fig. 22), 2.1 times longer than high (Fig. 23). Pronotal groove wide, deep and scrobiculate, pronotal carina distinct. Propleuron smooth. Mesoscutum slightly transverse, 0.7 times as long as wide. Mesoscutal lobes smooth, notauli deep and scrobiculate, obscured in an irregularly rugose median area before reaching the scuto-scutellar suture. Prescutellar sulcus with three distinct carinae. Scutellar disc smooth and triangular. Mesopleuron smooth. Precoxal sulcus deep and scrobiculate, running along basal two-thirds of mesopleuron. Subalar sulcus deep and rugose. Metanotum with a distinct median carina-like projection. Metapleuron entirely areolate-rugose. Propodeum with two divergent carinae running from median anterior edge delimiting two smooth dorsolateral areas; area beyond these carinae almost uniformly areolate-rugose.

Wings: fore wing (Fig. 28) length 3.0 mm, length/width ratio 3.85; vein 1cu-a postfurcal to vein 1M; veins 2RS/2M ratio 0.5.

Legs: fore tibia with a row of spines. Hind coxa transversally striate dorsally, smooth ventrally, with a distinct basoventral tubercule.

Metasoma: Basal sternal plate/length of first tergum 0.6. First metasomal tergite 2.2 times longer than apically wide (Fig. 29). Second median tergite longitudinally costate on basal three fourths, smooth apically. Suture between second and third median tergites slightly sinuate. Remaining tergites smooth and polished. Ovipositor length 1.4 mm, 0.9 times as long as metasoma.

Body length 2.6–3.8 mm. Temple/eye length ratio in dorsal view 0.16–0.33. Antenna with 22-27 flagellomeres, white subapical band composed of two to four flagellomeres. In smaller specimens, rugose median area of mesoscutum reduced, though notauli never clearly distinguishable at posterior edge of mesoscutum, obscured among rugosities. Prescutellar sulcus sometimes with para-median carinae reduced, thus only the median carina is clearly distinguishable. Fore wing length 2.2–2.9 mm, length/width ratio 2.9–3.9 times its maximum width. Veins 2RS/2M ratio 0.5–0.55. Basal sternal plate 0.53–0.68 times length of first metasomal tergum. Ovipositor length 2.0–2.1 mm.

Body length 2.5–3.5 mm. Malar space 0.28. Temple/eye length ratio (dorsal view) 0.29–0.39. Flagellomeres 21–26 either entirely brown (Chiapas) (Fig. 27) or with two apical flagellomeres whitish (Oaxaca) (Fig. 25). Median apex of mesoscutum slightly coriaceous. Venter of mesosoma coriaceous to slightly coriaceous. Metapleuron coriaceous, coriaceous to slightly rugose distally. Fore wing 1cu-a vein slightly postfurcal to vein1M.2RS/2Mratio 0.47–0.51. Basal sternal plate 0.54 times length of first metasomal tergite.

This species is known from cloud forests located in the Reserva el Triunfo, Chiapas, and Santiago Comaltepec, Oaxaca, in southeast Mexico.

Unknown.

This species has a considerable variation in the antennal color pattern. We had originally grouped the specimens assigned to this taxon in two morphospecies, each represented by the specimens from Chiapas and Oaxaca, respectively. Females from Oaxaca have a distinct apical white band composed of 6–8 flagellomeres (Fig. 24), whereas in males this apical band is smaller (Fig. 25). On the other hand, most females from Chiapas have a white antennal band that is subapical and is only composed of 2-4 flagellomeres (Fig. 26). However, one female that could not be sequenced has an apical band similar to the specimens from Oaxaca. Other external morphological features (e.g. sculpture of propodeum and first metasomal tergite) also varied but we could not find any correlation with the geographical provenance of the specimens.

There was no concordance between the corrected COI distances and the geographic provenance and morphological variation for the above specimens. Some of the specimens from Oaxaca had lower COI distances with those from Chiapas than with the remaining specimens from the same locality (0.38–0.76 and 1.7–1.9%, respectively). This incongruence suggests that the existence of incomplete lineage sorting or hybridization between two recently diverged, sympatric species (see below). We have followed a conservative approach and consider the members of the populations from Chiapas and Oaxaca as a single species. One of the specimens from Oaxaca (DNA voucher number CNIN573) has considerably higher COI distances compared with the remaining conspecific specimens (3.6–4.7%). However, it is morphologically undistinguishable and we thus placed it within S. tzeltal.

The name of this species refers to the Tzeltal ethnic group, descendant from the Mayans that inhabits Los Altos, a mountain region located in central Chiapas

Holotype (CNIN): female, Mexico, Chiapas, Mpo. Albino Corzo, Reserva el Triunfo, 15°39.428N, 92°48.67W, YPT, 16/XI/2001, Kovarik col., DNA voucher number CNIN-711, GenBank accession numbers KC821997 (COI), KC822257 (cyt b), KC822078 (EF-1alpha; not included in this work), KC822122 (wingless; not included in this work). Paratypes (CNIN, MACN): one female, five males, same data as holotype, DNA voucher numbers CNIN712-15, 18, GenBank accession numbers KX074181-84, 87 (COI), KX074190-93 (cyt b), KX074195-97, 200 (28S); two females, same data as holotype except 15°39.447N, 92°48.40W, 17-20/XI/2001, DNA voucher numbers CNIN720-21, GenBank accession numbers KX074188-89 (COI); two females, five males, Mexico, Oaxaca, Mpio. Santiago Comaltepec, 17.62836 -96.4672; 6-8/VI/2009, YPT, 1495m, A. Zaldívar, H.Clebsch, DNA voucher numbers CNIN457-59, 461-64, GenBank accession numbers JN870332-34, 36-39 (COI), JN870515, 17-19 (cyt b), JN870673-74, 76-79 (wingless; not included in this work); one female, four males, Mexico, Oaxaca, Mpio. Santiago Comaltepec, 17.59056 -96.39902; 8/VI/2009, bosque mesófilo, 1998-2141m, A. Zaldívar, H.Clebsch, DNA voucher numbers CNIN468-71, GenBank accession numbers JN870343-46 (COI), JN870524 (cyt b), JN870681-84 (wingless; not included in this work); one male, Mexico, Oaxaca, Mpio. Santiago Comaltepec, La Esperanza, 17.62661 -96.36950; 8/VI/2009, cloud forest, 1600m, A. López, DNA voucher number CNIN479, GenBank accession number JN870352 (COI), KX074194 (cyt b), JN870689 (wingless; not included in this work); two females, five males, Mexico, Oaxaca, Mpio. Santiago Comaltepec, 17.62334 -96.34669; 6/V/2009, bosque mesófilo, 1460m, A. Zaldívar, DNA voucher numbers CNIN379-80, 446-48, 452-53, GenBank accession number JN870324-26, 295-96 (COI), JN870509-11, 466 (cyt b), JN870630-31, 64-65 (wingless; not included in this work), KC822056-57 (EF-1alpha; not included in this work).

See diagnosis of S. tzeltal.

Body length 3.7mm (Fig. 30), fore wing 3.2mm; ovipositor sheaths 2.3mm. Colour: head uniformly brown, antenna brown, gradually darkening towards apex, except for a light band composed of most of the 19^th and the basal half of the 20^th flagellomeres (Fig. 34). Mesosoma uniformly dark brown, except for a slightly lighter area on median area of mesoscutum. Metasoma brown. Legs light brown, except fore and middle coxae, trochanters and trochantelli and hind trochantellus which are pale yellow; hind coxa and apical three fourths of hind femur dark brown. Wings hyaline; pterostigma and veins brown. Ovipositor sheaths brown.

Head: in anterior view 0.9 times as high as wide (Fig. 31), and 0.6 times as long as wide in dorsal view (Fig. 32). Clypeus, face, frons and vertex smooth and shining; temple smooth (Fig. 33). Eye 1.3 times higher than wide. Malar space height/eye height ratio 0.4. Temple/eye length ratio (dorsal view) 0.6. Antenna with 25 flagellomeres, first flagellomere five times longer than wide and about as long as the second one.

Mesosoma: 2.1 times longer than wide (Fig. 35) and 2.0 times longer than high (Fig. 36). Pronotal groove wide, deep, and scrobiculate, pronotal carina distinct. Propleuron smooth. Mesoscutum slightly transverse, 0.7 times as long as wide. Mesoscutal lobes smooth, notauli deep and scrobiculate, obscured in an irregularly rugose median area before reaching the scuto-scutellar suture. Prescutellar sulcus with three distinct carinae. Scutellar disc smooth and triangular. Mesopleuron smooth. Precoxal sulcus, deep and scrobiculate, running along basal two-thirds of mesopleuron. Subalar sulcus deep and scrobiculate-rugose. Metanotum with a distinct median projection. Metapleuron entirely areolate rugose. Propodeum with two divergent carinae running fron median anterior edge delimiting two dorsolateral areas, these areas are mostly smooth, but turn rugose areolate near carinae; beyond these carinae the propodeum almost uniformly areolate-rugose.

Wings: Fore wing length 2.9 mm, length/width ratio 3.3; vein 1cu-a slightly postfurcal to vein 1M; veins 2RS/2M ratio 0.5.

Legs: Fore tibia with a row of spines. Hind coxa transversally striate dorsally, smooth ventrally, with a distinct basoventral tubercle.

Metasoma: Basal sternal plate/length of first tergum 0.6. First metasomal tergite 2.1 times longer than apically wide (Fig. 37). Second median tergite longitudinally costate on basal three fourths, smooth apically. Suture between second and third median tergites almost straight. Remaining terga smooth and polished. Ovipositor length 2.4 mm, 1.1 times as long as metasoma.

Figures 36–37. 

Sergey tzotzil sp. n.; 36 mesosoma in lateral view 37 metasoma in dorsal view.

Males. Similar to female, slightly smaller and with antenna uniformly brown.

Known only from the type locality in El Triunfo, Chiapas, Mexico.

Unknown.

This species and S. tzeltal were collected in the same locality in Chiapas.

The name of this species refers to the Tzotzil ethnic group, descendant from the Mayans, who inhabits the Altos, a mountain region located in central Chiapas.

Holotype (CNIN): female, Mexico, Chiapas, Mpo. Albino Corzo, Reserva el Triunfo, 15°39.428N, 92°48.67W, YPT, 16/XI/2001, Kovarik col., DNA voucher number CNIN717, GenBank accession nos. KC821999 (COI), KC822259 (cyt b), KX074199 (28S), KC822124 (wingless, not included in this work), KC822080 (EF-1alpha; not included in this work). Paratype (CNIN): one male, same data as holotype; DNA voucher number, CNIN716, DNA voucher nos. KC821998 (COI), KC822258 (cyt b), KX074198 (28S), KC822123 (wingless, not included in this work), KC822079 (EF-1alpha; not included in this work).

Intraspecific corrected genetic divergences varied from 0 to 2.1 (excluding CNIN573), 0.27 to 3.18 and 0 to 0.33% for COI, cyt b and 28S, respectively. Interspecific distances within Sergey on the other hand ranged from 7.99 to 15.28, 12.64 to 13.6 and 0.17 to 0.5% for COI, cyt b and 28S, respectively.

The Bayesian phylograms derived from the separate COI and cyt b analyses are included in the Figure 38. The COI bayesian phylogram significantly supported the monophyly of the three described species. Sergey coahuilensis was recovered as sister to Heterospilus but without statistical support (PP = 0.5). A clade with the remaining species of Sergey (PP = 0.5) recovered S. cubaensis from Cuba (PP = 0.5) as sister to a S. tzotzil (Chiapas) + S. tzeltal (Chiapas and Oaxaca) clade (PP = 1.0). Within S. tzeltal, there were three non-significantly supported, subclades, two of which were composed of specimens from Comaltepec, Oaxaca, but with one of them being more closely related to the subclade containing the specimens from El Triunfo, Chiapas.

Figure 38. 

Bayesian phylograms showing the relationships recovered by the separate COI and cyt b analyses. Black circles near branches indicate clades supported by posterior probabilities ≥ 95%; hollow circles near branches indicate clades supported by posterior probabilities ≥ 90 and ≤ 94%.

The bayesian phylogram derived from the cyt b sequences yielded similar relationships with the COI topology. Again, some of the specimens of S. tzeltal from Comaltepec, Oaxaca were more closely related to the ones from El Triunfo, Chiapas (PP = 0.6) than with the remaining specimens from the same locality. The 28S tree was largely unresolved (phylogram not shown), with the sequenced specimens S. tzeltal and S. tzotzil grouped together (PP = 1.0). The reconstructed mt gene genealogies, together with the geographic provenance and morphological variation found in the specimens of S. tzeltal from Oaxaca and Chiapas suggests that this taxon could consist of two sympatric, recently derived lineages in which there is incomplete lineage sorting or hybridization. Further morphological and genetic studies will help to confirm the taxonomic status of the populations of S. tzeltal from the latter two Mexican regions.