Short Communication |
Corresponding author: Jens Zarka ( zarkajens@gmail.com ) Academic editor: Stefano Taiti
© 2022 Jens Zarka, Thomas Parmentier, Nicky Wybouw.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Zarka J, Parmentier T, Wybouw N (2022) Intersexuality in a natural population of the terrestrial isopod Porcellio scaber. In: De Smedt P, Taiti S, Sfenthourakis S, Campos-Filho IS (Eds) Facets of terrestrial isopod biology. ZooKeys 1101: 183-190. https://doi.org/10.3897/zookeys.1101.77212
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Intersex phenotypes are rarely observed in natural isopod populations and their expression is typically associated with infection of Wolbachia, a reproductive parasite that manipulates arthropod reproduction. During an intensive sampling effort of a natural population of the isopod Porcellio scaber, an adult individual was isolated that expressed both male and female traits. The intersex individual exhibited clearly developed external male genitalia and carried multiple eggs in its brood pouch. No Wolbachia infection could be identified in this individual, a result that needs to be approached with caution due to suboptimal DNA preservation for diagnostic PCR assays. Wolbachia were, however, detected in two adult females of the same population, and appear closely related to isolates that infect other terrestrial isopod species. This is the first demonstration that intersex phenotypes can arise under natural conditions in P. scaber.
Intersex phenotypes, Isopoda, sex-determination, Wolbachia
Sex-determination mechanisms regulate the sexual differentiation of organisms and are highly diverse across the animal kingdom. Sex-specific differentiation can rely on external environmental cues but can also be solely regulated by the segregation of genetic factors (
To date, Wolbachia-infected individuals have been described in at least 39 isopod species, with all Wolbachia isolates belonging to the Wolbachia B- or F-supergroup (
In genetic males, Wolbachia likely inhibit the development of the androgenic gland by either targeting the androgenic hormone promotor or the androgenic hormone receptor hereby feminizing the individual (
Here, we present the first record of an intersex Porcellio scaber collected from a natural population in Snellegem (Belgium) in August 2020. The individual carried a large number of eggs in its brood pouch, and can thus be considered as a functional female. However, the individual also possessed clearly developed external male genitalia.
We used cuboid pitfalls (25 cm × 7.5 cm × 8 cm) containing an approximately 1 cm bottom layer of plaster to collect isopods in Brugge, Snellegem, and Vleteren (
In addition to the intersex individual, we also isolated four adult females that exhibited normal sexual differentiation from the Snellegem population. Sterility was maintained by working in a biological safety cabinet. After washing the specimens twice in sterile water for 1 min, DNA was extracted from whole bodies using the Quick-DNA Universal kit (BaseClear, the Netherlands). DNA integrity was tested by amplifying a fragment of cytochrome c oxidase subunit I (COI) using the LCO1490 and HC02198 primers (
From a collection of 7,814 individuals, we found a P. scaber individual in the Snellegem population that carried eggs and, although egg viability was not ascertained, could be considered as a functional female (Fig.
A ventral view of the pleon of a normal female P. scaber B drawing of the ventral view of the male pleon of A. vulgare, similar in morphology to P. scaber C ventral view of the pleon of intersex P. scaber with the endopodite of the first pleopod marked with an arrow. Abbreviations: pp1 first pleopod; pp2 second pleopod; ex exopodite; en endopodite (B drawn from
Intersex individuals are rarely observed in natural populations of arthropods (
It is tempting to speculate that incomplete Wolbachia-mediated feminization caused the intersex phenotype in this individual. Wolbachia are widespread in P. scaber, infecting populations across Europe (
NW was supported by a BOF post-doctoral fellowship (Ghent University, 01P03420), TP by a CR FNRS fellowship (University of Namur, 30257865).