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Research Article
Redescription of Tylos maindroni Giordani Soika, 1954 (Crustacea, Isopoda, Oniscidea) based on SEM and molecular data
expand article infoValiallah Khalaji-Pirbalouty, Hamzeh Oraie, Carlos A. Santamaria§, Johann Wolfgang Wägele|
‡ Shahrekord University, Shahrekord, Iran
§ University of Tampa, Tampa, United States of America
| Leibniz-Institut zur Analyse des Biodiversitätwandels, Museum Koenig, Bonn, Germany
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Abstract

The woodlouse species Tylos maindroni Giordani Soika, 1954 (Crustacea, Isopoda, Oniscidea) is redescribed from the Persian Gulf based on light and scanning electron microscopy. This species differs from the closely related T. exiguus Stebbing, 1910, from the Red Sea (coasts of Sudan and Eritrea), and Socotra Island, by pereopod 1 superior margin without a prominent projection and pleopod 2 endopod 2.3 times as long as exopod, vs. 3.6 in T. exiguus. A distribution map for T. maindroni is provided. In addition, we studied the molecular differentiation of five populations of T. maindroni from the Persian Gulf, based on partial cytochrome c oxidase subunit I (COI) gene sequences. The results revealed low levels of population structuring between the analyzed populations.

Keywords

DNA barcoding, haplotype network, Isopoda, Persian Gulf, Redescription, SEM

Introduction

The isopod genus Tylos Audouin, 1826 has a worldwide distribution, with 21 species currently considered as valid (Boyko et al. 2008 onwards). Species in this genus are found in the marine sandy supralittoral zone, where animals can feed on algae and other organic material washed up on the beach by the waves (Kensley 1974). To avoid excessive predation by daytime predators (birds, crabs), feeding occurs at night (Schmalfuss and Vergara 2000). These animals are able to roll up into a perfect ball, with the antennae remaining inside the ball. Endoantennal conglobation can be also observed in Armadilliidae, Eubelidae, and Scleropactidae. Rolling up is not only a response to predators, but it can also help reduce water loss by about 35% (Schmalfuss and Vergara 2000; Sfenthourakis et al. 2020). According to Schmalfuss (2003), only two species of Tylos have been recorded from the northwestern areas of the Indian Ocean: T. exiguus Stebbing, 1910 from the Red Sea (coasts of Sudan and Eritrea) and the coasts of Socotra Island (Schmalfuss and Vergara 2000; Taiti and Ferrara 2004; Taiti and Checcucci 2010); and T. maindroni Giordani Soika, 1954 from the Gulf of Oman and the Persian Gulf. The original description of T. maindroni is brief and based on a single female from Muscat, Oman. Taiti and Ferrara (1991) later reported this species from Kuwait, Oman, and Iran (Busher coast), with illustrations of specimens from Kuwait. Recently, a molecular phylogenetic study including various species of the genus Tylos clearly revealed the existence of two distinct Tylos species along the coastal zones of the Arabian Peninsula (northwestern Indian Ocean): T. maindroni and T. exiguus (Hurtado et al. 2014).

Herein, we redescribe T. maindroni based on material from the Persian Gulf and provide new COI mtDNA sequence data.

Materials and methods

Morphological analyses

Specimens used in this study were collected from four coastal sites in the Persian Gulf, Iran during field expeditions from 2006 to 2021 (Fig. 1; Table 1). All specimens are held in the isopod collection of the Zoological Museum of Shahrekord University (ZMSU).

Table 1.

Samples of the Tylos maindroni from the Persian Gulf used in this study.

Museum number Voucher numbers Coordinates Collection date Locality GenBank Accession number COI
ZSMU 1206 1043 26°16'20.48"N, 55°17'28.00"E 03.01.2021 Greater Tunb Island OK513061
ZSMU 1206 1044 26°16'20.48"N, 55°17'28.00"E 03.01.2021 Greater Tunb Island OK513060
ZMSU 1205 1051 26°6'59.99"N, 54°26'10.88"E 30.12.2017 Faroor Koochak Island OK513062
ZMSU 1201 1098 26°42‘555"N, 54°14‘329"E 05.12.2008 Bandar-e-Charak OK513063
ZMSU 1201 C2 26°42‘555"N, 54°14‘329"E 05.12.2008 Bandar-e-Charak OK513064
ZMSU 1202 B1 27°07‘113"N, 53°01‘418"E 30.01.2006 Banda-e Bostaneh OK513065
ZMSU 1202 B2 27°07‘113"N, 53°01‘418"E 30.01.2006 Banda-e Bostaneh OK513066
Figure 1. 

Map showing geographic distribution of Tylos maindroni Giordani Soika, 1954 A the Persian Gulf and Gulf of Oman B the Persian Gulf. Green circles = in the Persian Gulf, Red circle = type locality.

Specimens prepared for SEM were washed in a chilled 1% sodium acetate solution for 10 minutes, then cleaned for 10–20 seconds in an ultrasound cleaner in a weak solution of jewelry soap and distilled water to remove sediment and debris adhering to the cuticle. Specimens were dehydrated in an ethanol series (70, 75, 80, 85, 90, 95, 100%; 20 minutes per treatment). Specimens were transferred through ethanol and hexamethyldisilazane (HMDS) solutions (ethanol: HMDS ratios were 2:1, 1:1, 1:2) and finally into 100% HMDS (20 minutes per treatment). All samples were transferred to fresh HMDS, which evaporated overnight. Specimens were mounted on stubs using double adhesive carbon spots before being coated with gold in a sputter coater to 40 nm thickness. Micrographs were taken using a Hitachi S-2460N SEM at Zoologisches Forschungsmuseum Alexander Koenig in Bonn, Germany. Color images were taken using a Zeiss AxioCam ERc5s camera mounted on a Zeiss Stereomicroscope (Stemi 508).

Molecular analyses

We extracted genomic DNA from the legs of seven specimens, 1–2 individuals per locality, using the Aron-Gene Tissue DNA Extraction kit (Aron-Gene, Iran) following the manufacturer’s protocol. A 536 base pair fragment of the mitochondrial Cytochrome Oxidase I (COI) gene was PCR-amplified using the LCO-1490 and HCO-2198 primer pair under standard conditions (Folmer et al. 1994). The PCR solution consisted of a 10 µl PCR Master Mix (SinaClon BioScience, Iran), 2 µl of template DNA (~50 ng), 1 µl of each primer (concentration 10 pm/ml), and 6 µl of nuclease-free water for a total volume of 20 µl. PCR products were examined using gel electrophoresis on 1% agarose gels, with positive PCR amplifications sequenced on an ABI 3130XL automated sequencer. We assembled, inspected, and edited sequences using Bioedit v.7.0.5.3.

Once assembled and edited, sequences produced in this study were combined with previously published COI sequences of T. maindroni as well as other Tylos species, provided that these sequences were > 500-bp long. Information for publicly available sequences included in this study can be found in Table 2. Sequences were aligned using the online MAFFT server (Katoh et al. 2019) and default settings. The resulting alignment was trimmed to remove end gaps. No evidence suggestive of pseudo-genes was observed in the final alignment. Given the high levels of divergence amongst Tylos species and differences in sequence lengths across studies, we re-aligned the COI sequences for T. maindroni individuals separately.

Table 2.

GenBank Accession information for sequences used in this study. Accession numbers of sequences produced in this study are in bold.

Number of individuals GenBank Acc. No
T. maindroni 8 KJ468116; OK513060OK513066
T. capensis 33 MZ540108-MZ540140
T. chilensis 1 KJ468109
T. exiguous 1 KJ468112
T. granulatus 179 MK603245-MK603423
T. granuliferus 123 AB763432-AB763552; KJ468113-KJ468114
T. marcuzzii 1 KJ468118
T. niveus 1 KJ468120
T. opercularis 1 KJ468121
T. punctatus 23 KF007550-KF007555; KF007571-KF007574; KF007582-KF007586; KF007596-KF007598; KF007607-KF007608; KF007686-KF007688
Tylos sp. BOLD:ACM2291 1 KJ592778
Tylos sp. clade B* 1 KF007644
Tylos sp. clade C* 1 KF007626
Tylos sp. clade D* 1 KF007575
Tylos sp. clade F* 2 KF007689-KF007690
Tylos sp. clade G* 13 KF007576; KF007654; KF007657; KF007669-KF007671; KF007679-KF007680; KF007685; KF007698; KF007711-KF007712; KF007718
Tylos sp. clade H* 9 KF007599; KF007609-KF007611; KF007615-KF007616; KF007646-KF007648
Tylos sp. clade I* 7 KF007569; KF007664; KF007667-KF007668; KF007705; KF007713; KF007715
Tylos sp. hachijoMN12 1 AB763553
Tylos sp. outgroup* 1 KF007724
T. spinulosus 1 KJ468125
T. wegeneri 1 KJ468126

We used ASAP (Puillandre et al. 2021), a distance-based species delimitation approach, to determine if all T. maindroni sequences were assigned to a single species cluster. This analysis was carried out on the ASAP web portal (https://bioinfo.mnhn.fr/abi/public/asap/) under the Kimura (K80 or K2P) model (Kimura 1980) and a ts/tv ratio of 2.0. All other settings were as default. We estimated pairwise genetic distances with the Kimura-2-Parameter (K2P) correction in MEGA v11.0.10 (Tamura et al. 2021).

Lastly, we visualized relationships between T. maindroni COI haplotypes by reconstructing branch connections using the TCS network option (Clement et al. 2002) of PopArt v1.7 (Leigh and Bryant 2015), with a 95% connection limit.

Systematic account

Order Isopoda Latreille, 1817

Suborder Oniscidea Latreille, 1802

Family Tylidae Milne-Edwards, 1840

Tylos Audouin, 1826

Type species

Tylos latreillii Audouin, 1826; from an unspecified location in Egypt (type locality); but current status is a nomen dubium (Taiti and Ferrara 1996: 460).

Diagnosis

A diagnosis for the genus was published by Schmalfuss (2000).

Tylos maindroni Giordani Soika, 1954

Figs 2, 3, 4, 5, 6

Tylos maindroni Giordani Soika, 1954: 76, figs 8, 9, pl. 10, Oman Sea, Muscat (type locality); Ferrara and Taiti 1986: 94; Taiti and Ferrara 1991: 213, fig. 3; Taiti et al. 2000: 148; Hurtado et al. 2014: 3, fig. 1.

Material examined

7 ♂♂ (5.1 to 9.8 mm), 3 ♀♀ (5.5, 8.5, 10 mm), the Persian Gulf, Bandar-e-Charak, sandy shore, under wood block and rubbish on sand, 05 Dec. 2008, 26°42'555"N, 54°14'329"E, coll. V. Khalaji (ZMSU 1201); 8 ♂♂ (5 to 8 mm), 6 ♀♀ (6 to 9.2 mm), Bandar-e-Bostaneh, sandy shore, 03 Jan. 2006, 27°07'113"N, 53°01'418"E, coll. R. Naderloo (ZMSU 1202); 1 ♀ (12.2 mm), Bandar-e-Lengeh, sandy beach, beneath wood, 03 May 2010, 26°34'10"N, 54°54'21"E, coll. V. Khalaji (ZMSU 1203); 2 ♀♀ (9 and 10 mm), Kish Island, northern coast, Derakht-e-Sabz, 24 Jun. 2006, 26°34'102"N, 53°58'098"E, coll. V. Khalaji (ZMSU 1204); 3 ♂♂ (9 to 11mm); 8 ♀♀ (8 to 10 mm), Faroor Koochak Island, rocky, sandy western coast, 30 Dec. 2017, 26°65'999"N, 54°26'108"E, coll. V. Khalaji (ZMSU 1205); 10 ♀♀ (7 to 11mm), 5 ♂♂ (7.7 to 11 mm), Greater Tunb Island, sandy beach, 03 Jan. 2021, 26°16'20.48"N, 55°17'28.00"E, coll. V. Khalaji and M. Majidi (ZSMU 1206).

Redescription of male

(from the Persian Gulf). Color yellowish, or light brown dorsally with small, dark, pigmented dots of various densities (Fig. 2A–D), about 2.5 times as long as greatest width. Cephalon with a weak domed process on each side between eyes. Epistome triangular with narrowly rounded apex, labrum with rows of small tubercles, as figured (Fig. 3B). Eyes composed of 36–38 ommatidia in adults (Fig. 3E). Coxal plates 2–5 with rounded margin, coxal plates 6–7 rectangular with strait margin. Pleotelson framed by pleonite 5 laterally, distal margin with small setae, length about 0.55 times width (Fig. 3F, G).

Figure 2. 

Tylos maindroni Giordani Soika, 1954 A, B male from the Persian Gulf, Bandar-e-Charak (ZMSU 1201) C, D female from the Persian Gulf, Bandar-e-Charak (ZMSU 1201) E female from Faroor Koochak Island (ZMSU 1205) F male from Greater Tunb Island (ZSMU 1206).

Antennula (Fig. 3C). Small, disolateral and apical margins straight, medial margin concave, covered with cuticular scales, about 1.3 times as long as greatest width.

Figure 3. 

Tylos maindroni Giordani Soika, 1954, male from the Persian Gulf, Bandar-e-Charak, scanning electron micrographs A head, frontal view B epistome C antennule D antenna E head, dorsal view F pleon and pleotelson, caudal view G pleon and pleotelson, dorsal view.

Antenna (Fig. 3D). Extending to posterior margin of pereonite 1, basal peduncular articles 2–5 increasing in length; article 5 about 1.3 times as long as article 4; flagellum with 4 articles, distal article smallest, apex with cone-like tuft of setae.

Left mandible (Fig. 4D). Pars incisiva with three cusps; lacinia mobilis with three cusps and 2 penicils; pars molaris with flat grinding surface and with a tuft of numerous hair-like setae on margin.

Figure 4. 

Tylos maindroni Giordani Soika, 1954, male from the Persian Gulf, Bandar-e-Charak, scanning electron micrographs A maxillule, lateral endite B maxillule, medial endite C maxilla D left mandible E right mandible F maxilliped.

Right mandible (Fig. 4E). Pars incisiva with three cusps; lacinia mobilis with some small, sharp cusps (about 8) and 2 penicils; pars molaris grinding surface smaller than on left mandible, with a tuft of numerous hair-like setae on proximal margin.

Maxillule (Fig. 4A, B). Lateral endite with 12 robust, simple or serrate setae; mesial endite with 2 subapical and 1 apical penicils.

Maxilla (Fig. 4C). Apical margin round, densely setose.

Maxilliped (Fig. 4F). Endite apical margin with 6 robust, simple setae, 3 large penicils, and 2 smaller penicils; palp of five articles, articles 2–5 each bearing a tuft of marginal, rod-like setae.

Pereopod 1–7 (Figs 5A–F, 6A). All with a rich armature of robust setae; pereopod 1 basis about 2.1 times as long as wide, ventral margin with a weak extension; pereopods 2–5 with longer basis; pereopod 7 (Fig. 6A) basis about 2.3 times as long as wide, with water conducting scale-rows.

Figure 5. 

Tylos maindroni Giordani Soika, 1954, male from the Persian Gulf, Bandar-e- Charak, scanning electron micrographs A–F pereopods 1–7, respectively.

Pleopod 2 (Fig. 6B). Exopod equipped with open lungs consisting of 8 pores, distal margin with cuticular scale. Endopod elongated, well extended beyond exopod distal margin, apical part bearing hand-like scales with 2–7 “fingers” that are directed proximally, proximal third covered with cuticular scales.

Figure 6. 

Tylos maindroni Giordani Soika, 1954, male from the Persian Gulf, Bandar-e- Charak, scanning electron micrographs A pereopod 7 B pleopod 2 C pleopod 3 D uropod.

Pleopod 3 (Fig. 6C). Exopod equipped with open lungs consisting of 9 pores.

Uropod (Fig. 6D). Protopod (peduncle) with straight medial margin, disto-lateral margin with 6 small marginal setae, length about 1.62 greatest width; exopod small, about 0.23 times length of protopod, covered with small setae medially.

Female (Fig. 2C, D). Apart from sexual characters, similar to male.

Distribution

Oman, the Persian Gulf (Kuwait; Bandar-e-Charak, Bandar-e-Bostaneh, Bandar-e-Lengeh, Kish, Greater Tunb, and Faroor Koochak Islands, Iran)

Results

Genetic differentiation

We obtained seven 534-bp long COI sequences from T. maindroni individuals from four locations across the Persian Gulf coastline of Iran. These sequences were combined with a previously published COI sequence of T. maindroni from Kuwait (GenBank Acc. KJ468116; BIN: BOLD: ACQ3230). We identified four highly similar COI haplotypes as indicated by K2P divergences (0.0–0.4%, 1–3 nucleotide differences, Fig. 7). These haplotypes, however, were highly divergent from those found in other Tylos species (16.2–33.9% COI K2P divergences, Table 3).

Table 3.

Average COI K2P divergences amongst Tylos species included in this study.

T. maindroni T. punctatus Tylos sp. BOLD:ACM2291 Tylos sp. clade F* Tylos sp. clade D* Tylos sp. clade G* Tylos sp. clade H* Tylos sp. clade I* Tylos sp. clade C* Tylos sp. clade B* Tylos sp. outgroup* T. niveus T. granulatus T. capensis T. marcuzzii T. exiguus T. opercularis T. chilensis T. spinulosus Tylos sp. hachijoMN12 T. granuliferus T. wegeneri
T. maindroni < 0.5
T. punctatus 20.5 < 5.8
Tylos sp. BOLD: ACM2291 20.5 0.1 N/A
Tylos sp. clade F* 19.2 14.9 14.8 0.0
Tylos sp. clade D* 17.7 12.5 12.4 11.0 N/A
Tylos sp. clade G* 18.2 13.6 13.6 13.2 12.5 < 6.2
Tylos sp. clade H* 18.6 14.0 14.1 12.6 12.2 4.6 0.0
Tylos sp. clade I* 18.9 14.2 14.3 13.6 13.0 5.6 4.5 0.0
Tylos sp. clade C* 21.2 15.6 15.5 12.1 14.2 13.5 14.0 13.9 N/A
Tylos sp. clade B* 20.4 12.9 12.8 14.5 13.1 13.4 13.1 13.4 13.1 N/A
Tylos sp. outgroup* 19.9 16.0 15.9 16.7 18.2 15.8 15.9 16.3 18.4 14.7 N/A
T. niveus 17.7 16.0 15.8 15.1 16.8 15.5 17.1 16.8 16.6 15.9 15.6 N/A
T. granulatus 17.4 15.9 15.9 15.7 15.0 15.2 15.9 15.8 18.5 16.7 19.1 15.8 < 13.2
T. capensis 19.5 16.3 16.3 17.9 17.7 17.5 18.4 17.7 20.8 17.8 19.0 19.8 12.2 < 2.8
T. marcuzzii 21.5 17.8 17.8 21.6 22.7 20.2 20.9 21.3 19.9 17.3 18.0 20.6 19.4 19.6 N/A
T. exiguus 20.7 21.3 21.2 20.5 19.2 18.7 18.8 19.2 21.9 20.5 21.0 22.9 18.9 19.1 23.1 N/A
T. opercularis 25.4 25.5 25.5 21.9 25.2 25.1 25.2 25.2 27.3 25.8 23.6 19.2 23.3 23.3 26.0 23.0 N/A
T. chilensis 25.6 23.7 23.6 26.2 25.4 24.9 25.0 24.6 24.8 25.1 24.3 25.5 22.7 24.8 24.4 24.1 29.1 N/A
T. spinulosus 23.2 21.1 21.0 22.6 22.2 21.0 21.4 22.7 22.8 21.3 23.5 23.0 20.9 22.1 27.0 22.0 31.3 13.7 N/A
Tylos sp. hachijoMN12 23.9 27.3 27.3 25.1 26.6 25.2 27.4 27.0 25.7 24.9 26.2 21.2 25.2 25.8 29.7 28.2 24.1 33.5 28.3 N/A
T. granuliferus 28.9 30.5 30.4 27.1 25.6 27.6 27.2 27.2 28.0 27.5 31.5 27.3 25.6 26.2 32.0 26.3 24.4 32.2 30.3 24.4 < 25.2
T. wegeneri 26.2 28.7 28.7 27.3 27.2 26.3 25.4 27.7 26.2 26.0 27.7 27.4 25.9 26.0 25.7 27.1 26.4 27.8 27.3 26.5 29.2 N/A
Figure 7. 

Haplotype networks for the COI mitochondrial gene fragment of Tylos from the Persian Gulf. Colors correspond to locations as indicated in figure. Dashes along branches represent the number of nucleotide differences between haplotypes. Frequency of haplotype recovery represented through relative sizes of circles.

Combining the T. maindroni sequences with other previously published sequences of the genus Tylos resulted in a 517-bp long alignment containing 410 sequences. ASAP analyses of this dataset identified two partitioning schemes with nearly similar numbers of hypothetical species groups (23 and 24), threshold distances (0.068107 and 0.051440), and low ASAP scores (7 and 8). This last measure reflects both the p-value and the relative barcode gap width rank for a given partitioning scheme, with lower values reflecting stronger support for a given partitioning scheme. All COI haplotypes from T. maindroni individuals were placed in a single cluster that included no sequences from other Tylos species, regardless of the partitioning scheme.

Discussion

Tylos maindroni was first described by Giordani Soika in 1954; however, the original description was brief and did not include a discussion or illustration of characters used in the taxonomy of this genus. A later work by Taiti and Ferrara (1991) suggested that T. maindroni’s geographic range extends into the Persian Gulf, including locations on the coasts of Kuwait and Iran, but additional work remains necessary to clarify the status of this species and its geographic range. Additionally, considering the high levels of genetic divergence reported in several coastal isopod taxa (Hurtado et al. 2013; Khalaji-Pirbalouty and Raupach 2014, 2016; Raupach et al. 2014; Hurtado et al. 2017; Santamaria et al. 2017; Greenan et al. 2018; Hurtado et al. 2018; Santamaria 2019), it would be important to determine if T. maindroni harbors cryptic diversity in its native range.

Our Persian Gulf specimens correspond morphologically quite well to the brief description and illustrations of T. maindroni from Oman by Giordani Soika (1954) and from Kuwait by Taiti and Ferrara (1991). Nevertheless, there is a slight difference in the number of lung pores on the exopod of the pleopods: the exopod of pleopod 2 has 8 pores rather than 7 and the exopod of pleopod 3 has 9 pores rather than 8. Tylos maindroni is morphologically most similar to T. exiguus Stebbing, 1910, a Red Sea species shown by Hurtado et al. (2014) to be a sister taxon to T. maindroni based on several mitochondrial markers. The former species differs from T. maindroni by having pereonite 1 posterior margin with a distinctly deeper concavity at the lateral side, pereopod 1 superior margin with a prominent projection, and pleopod 2 endopod 3.6 times as long as exopod (vs. 2.3 times in T. maindroni).

Molecular data are in concordance with the above findings. All Tylos specimens that were morphologically identified as T. maindroni have highly similar COI haplotypes differing by a maximum of three positions (K2P distances amongst haplotypes < 0.5%). Furthermore, sequences recovered from T. maindroni individuals were highly divergent from all other COI sequences recovered from other Tylos species including T. exiguus (16.2–33.9% COI K2P). Not surprisingly, all T. maindroni haplotypes were assigned to a single species cluster in species delimitation analyses carried out in ASAP, regardless of the partitioning scheme.

The low level of diversification herein reported between individuals of T. maindroni collected at Persian Gulf locations stands in contrast with those reported for other coastal oniscid taxa (Khalaji-Pirbalouty and Raupach 2014, 2016; Raupach et al. 2014; Hurtado et al. 2017; Santamaria et al. 2017; Greenan et al. 2018; Hurtado et al. 2018; Santamaria 2019), including other Tylos species (Hurtado et al. 2013). For instance, the molecular characterizations of Tylos populations from the Gulf of California showed genetic differentiation in COI sequences ranging from 3.6 to 17.3%, indicating long-standing isolation of the populations in the region as well as the possible presence of cryptic species (Hurtado et al. 2013). Similarly, T. granulatus populations in South Africa have shown to harbor two highly divergent mitochondrial lineages (Mbongwa et al. 2019). In contrast to this, the COI K2P divergences observed in T. maindroni were less than 0.5%.

The low levels of genetic divergence within T. maindroni in the Persian Gulf is likely a reflection of the young age of this marine waterbody. Although there is disagreement on the extent of the Persian Gulf coastline during the Holocene and Late Pleistocene (Sissakian et al. 2020), the Gulf Basin is thought to have been free of marine influence up until the last glacial maximum ~18,000 ya., with marine flooding due to rising sea levels and glacial displacement starting ~14,000 ya (Lambeck 1996). Thus, the geology of the region suggests that the ancestor to T. maindroni populations in the Persian Gulf area invaded the Gulf in the past ~14,000 years. Alternatively, the low divergence levels between COI sequences reported herein may be the result of infection with Wolbachia. Infection with this endosymbiotic bacterium has been proposed to reduce mitochondrial polymorphisms in arthropods, including isopods (Marcadé et al. 1999; Xiao et al. 2012; Delhoumi et al. 2019; but see Tang et al. 2019). We cannot determine whether Wolbachia have reduced mitochondrial diversity in T. maindroni in the Persian Gulf as we did not test for the presence of Wolbachia in our specimens. However, given the recent geological and hydrological history of the Persian Gulf, we propose that the low levels of divergence in T. maindroni reported herein are likely the result of the young age of the modern Persian Gulf. Nevertheless, future work remains needed to conclusively discern between these explanations. Future studies also remain needed to clarify the origins and evolution of T. maindroni in the region. The closest extant relative of T. maindroni in the Persian Gulf is T. exiguus (Hurtado et al. 2014), suggesting that the Persian Gulf populations of T. maindroni likely originated from an ancestor inhabiting coastal habitats in the Indian Ocean basin. As our sampling did not include T. maindroni populations from the Indian Ocean, future work would be best served by incorporating these populations.

Acknowledgements

We would like to thank Dr. Saskia Brix (Senckenberg Research Institute, Germany), Dr. Michael Raupach (Bavarian State Collection of Zoology, Munich, Germany) and one anonymous reviewer for their constructive suggestions and helpful comments. This study is a part of the Biodiversity and Conservation project under a joint program by the Ministry of Science, Research and Technology of Iran and the Leibniz Association, Germany. Financial support for this study was provided by the Iran University of Science and Technology, International Affairs, Shahrekord University, Iran and the Leibniz Association, Germany.

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