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Research Article
Order Euryalida (Echinodermata, Ophiuroidea), new species and new records from the South China Sea and the Northwest Pacific seamounts
expand article infoHasitha Nethupul§, Sabine Stöhr|, Haibin Zhang
‡ Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
§ University of Chinese Academy of Sciences, Beijing, China
| Swedish Museum of Natural History, Stockholm, Sweden
Open Access

Abstract

Ophiuroids were collected by the manned submersible ‘Shenhaiyongshi’ from the deep-sea seamounts in the South China Sea and Northwest Pacific regions at 602–1920 m depth, during 2018 to 2020. A total of nine species was identified, including two new species and seven new records from the South China Sea and one new record from the Northwest Pacific region. Two new species are described as Asteroschema shenhaiyongshii sp. nov. and Asteroschema domogranulatum sp. nov. The seven new records included five species from the genus Asteroschema, and one species each from the genera Asterostegus and Astrodendrum. Comprehensive descriptions of morphological features are provided, including characteristics of the arm skeleton, as well as a phylogenetic analysis based on 16S and COI sequences. Intraspecific genetic distance ranges of Euryalida species from the present study were 0.34% to 1.38%, which was relatively low compared to other orders in the class Ophiuroidea. The present study suggests a high probability that species of the order Euryalida are more widely spread around the Indo-Pacific region than previously expected.

Keywords

Asteroschema, COI, molecular phylogeny, morphology, SEM, taxonomy

Introduction

The order Euryalida Lamarck, 1816 (basket stars and snake stars) includes the families Euryalidae Gray, 1840, Asteronychidae Ljungman, 1867, and Gorgonocephalidae Ljungman, 1867, and these include the species with largest known body size in the class Ophiuroidea, the brittle stars (Stöhr et al. 2021). The majority of the Euryalida are epizoic, living attached to hosts, such as corals, gorgonians, and sponges (Baker 1980). Currently, the order Euryalida contains 193 accepted species within 48 genera (Stöhr et al. 2021). The largest families are Euryalidae and Gorgonocephalidae with 95% of all Euryalida (Stöhr et al. 2021). These two families include 44 genera (Euryalidae 11 genera, Gorgonocephalidae 33 genera).

This study presents species in the genera Asteroschema Örsted & Lütken in Lütken, 1856 and Asterostegus Mortensen, 1933 from Euryalidae, and Astrodendrum (Döderlein, 1902) from family Gorgonocephalidae, found in the South China Sea and on Northwest Pacific seamounts.

Asteroschema is one of the largest genera in the Euryalidae, but it is still ill-defined due to limited published information and high morphological similarity between the species. Currently, 33 species are included in Asteroschema and the most recently described one was Asteroschema sampadae Parameswaran & Abdul Jaleel, 2012 from the Indian Ocean. Most of the species are pentamerous, but two hexamerous species have been recorded from New Zealand waters (Asteroschema wrighti McKnight, 2000 and Asteroschema bidwillae McKnight, 2000). Previous studies differentiated Asteroschema species based on epidermal ossicle shape and arrangement on the disc and arms, size variance and shape of inner and outer arm spines, and starting point of the second arm spine on the proximal region of the arm (Okanishi and Fujita 2009; Parameswaran and Jaleel 2012). The term epidermal ossicle has been used for small superficial, often granule-like, skeletal elements on the disc and arms (Okanishi and Fujita 2009). Echinoderm skeleton generally develops in the dermis (Byrne 1994), but it is unknown if these ossicles originate in the epidermis or in the dermis, and their possible homology with granules or spines in non-euryalid groups is also unknown. Epidermal thus does not refer to the place of origin of these ossicles, but to their position in adult specimens. Asteroschema species have been divided into three groups according to the shape of their epidermal ossicles such as: species with only granular ossicles, with conical and granular ossicles, and domed and with plate-like granular ossicles (Okanishi and Fujita 2009).

The genus Asterostegus includes only three species and is morphologically related to the genus Astroceras Lyman, 1879, but differs in having the oral shield replaced by several small interradial plates (McKnight 2003; Okanishi and Fujita 2014). Understanding morphological variations and diversity of Asterostegus is limited due to a lack of material (Okanishi and Fujita 2014). In the present study, Asterostegus maini McKnight, 2003 from the South China Sea is recorded as the first record since the holotype, but recent studies of Asterostegus included detailed descriptions of all three species (Okanishi and Fujita 2014). However, this study includes the intraspecific morphological variation among A. maini specimens between the South China Sea and South Pacific waters, and the development of morphological characters relative to size variations. Lastly, the genus Astrodendrum is widely distributed from the Indo-Pacific to South Africa, and includes six species. It differs from other genera in the family Gorgonocephalidae by having external ossicles of various shapes on the disc, and by lacking calcareous plates on the lateral disc margin (Okanishi and Fujita 2018).

The present study covers deep waters around the South China Sea (Xisha and Zhongsha Islands) and in the Northwest Pacific region (southwest of Guam Island). Here, we present an account of the Asteroschema, Asterostegus, and Astrodendrum species collection, with descriptions of new species and new records. Our goal is to present a detailed documentation of the morphological features of these species, to complement the limited original descriptions and the lack of figures in the literature. We present the first ever comprehensive tabular key for all species in the genus Asteroschema. Two new species are described and seven species are redescribed, including seven new records from the South China Sea and one new record from the Northwest Pacific, all richly illustrated. DNA barcoding was used to identify ophiuroid species in the past two decades (Ward et al. 2008; Hoareau and Boissin 2010; Okanishi et al. 2011, 2018; Okanishi and Fujita 2013). Hence, we use barcoding to test our morphological identifications and to understand the interrelationships within genera. This study also provides biodiversity information of Euryalida species living on seamounts, which may be useful for further studies of euryalid diversity and biogeography.

Materials and methods

Sample collection

Ophiuroid specimens were collected by the manned submersible vehicle ‘Shenhaiyongshi’, from 602 to 1920 m depth (Fig. 1). Most of the specimens were frozen at -80°C without preservation fluid, then transported to the Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences (CAS), Sanya, China, for further analysis. The samples were sorted and identified by using literature (Pallas 1788; Ljungman 1871; Lyman 1869, 1872, 1875, 1878, 1879, 1882, 1883; Lütken and Mortensen 1889; Alcock 1894; Verrill 1894, 1899; Koehler 1904, 1906, 1907, 1914, 1930; Matsumoto 1911, 1915, 1917; H. L. Clark 1915, 1916b, 1917, 1939, 1941; Mortensen 1924; A. H. Clark 1916a, 1949; Döderlein 1911, 1927, 1930; Murakami 1944; Baker 1980; Guile 1981; Peterson 1985; McKnight 2000; Liao 2004; Mah et al. 2009; Pawson et al. 2009; Parameswaran and Jaleel 2012; Smirnov et al. 2014; Olbers et al. 2015) and by molecular analysis.

Figure 1. 

Collecting stations in this study A, A1 South China Sea (Hainan, Xisha, and Zhongsha Islands) B, B1 Northwest Pacific (southwest of Guam). Source: International Hydrographic Organization and Sieger (2012).

Morphological analysis

Specimens were photographed through a dissecting stereo microscope (OLYMPUS SZX7) or with a digital camera (Canon EOS 6DII) to identify morphological characters. Arm skeletons were examined with a scanning electron microscope (SEM) Phenom ProX. Skeletal elements were prepared by using undiluted NaOCl to dissolve the soft tissue of part of an arm. The excess NaOCl in skeletal elements was removed by repeated flushing with distilled water. Then, the ossicles were mounted on a stub using dissolved carbon tapes. Holotypes, paratypes and all other specimens are deposited at the Institute of Deep-sea Science and Engineering (CAS), Sanya, China. The terms used to describe ophiuroids follow previous authors (Martynov 2010; Stöhr 2011, 2012; Okanishi and Fujita 2014, 2018; O’Hara et al. 2017; Hendler 2018; Stöhr and O’Hara 2021). We define granules and spines as articulated ossicles on plates or scales, but tubercles as non-articulated stereom outgrowth, following Stöhr et al. (2012) and Goharimanesh et al. (2021). Following Turner et al. (2021), we utilize the term “pedicellarial bands” for what was previously known as “girdle bands” and for the “girdle hooklets” we use the term “pedicellariae”.

Molecular analysis

We extracted DNA from identified specimens by using the TIANamp Marine Animals DNA kit (TianGen, Beijing) following the manufacturer’s protocol. We sequenced cytochrome c oxidase I (COI) and the 16S partial gene for phylogenetic analysis by amplifying primer sets, with suitable PCR cycle (Suppl. material 1: Table S1) (Hoareau and Boissin 2010; Okanishi and Fujita 2013). Total PCR mixture was 50 μL volume, containing 25 μL Premix Taq with 1.25 U Taq, 0.4 mM of each dNTP and 4 mMMg2+ (Ex Taq version, Takara, Dalian, China), 0.5 μM each of the primers and approximately 100 ng template DNA. We performed electrophoresis using a 1.0% agarose gel and the NanoDrop 1000 (Thermo Scientific, Waltham, MA, USA) to assess PCR product quality of the specimens. PCR products were sequenced in both directions on an ABI3730 DNA Analyzer. All new sequences were deposited at NCBI GenBank (Table 1).

Table 1.

Localities, voucher information, and GenBank accession numbers for all specimens used in this study.

Species Locality Voucher number COI 16S
Asteroschema shenhaiyongshii sp. nov. South China Sea, near Xisha islands IDSSE-EEB-SW0086 OK044292 OL712208
Asteroschema bidwillae New Zealand MVF188856 KU895077 -
Asteroschema rubrum South China Sea, near Zhongsha islands IDSSE-EEB-SW0071 OK044293 OL712209
Asteroschema rubrum South China Sea, near Zhongsha islands IDSSE-EEB-SW0072 OK044294 OL712210
Asteroschema rubrum South China Sea, near Zhongsha islands IDSSE-EEB-SW0073 OK044295 OL712211
Asteroschema tubiferum New Zealand MVF188857 KU895076 -
Asteroschema tubiferum Mariana Trench, Southeast of Guam Isl. IDSSE-EEB-SW0078 OK044296 OL712212
Asteroschema tubiferum South China Sea, near Zhongsha islands IDSSE-EEB-SW0106 OK044297 OL712213
Asteroschema tubiferum South China Sea, near Zhongsha islands IDSSE-EEB-SW0077 OK044298 -
Asteroschema cf. lissum Mariana Trench, Southeast of Guam Isl. IDSSE-EEB-SW0081 OK044299 OL712207
Asteroschema cf. lissum South China Sea, near Zhongsha islands IDSSE-EEB-SW0079 OK044300 -
Asteroschema salix Australia TOH_666 HM400451 -
Asteroschema salix South China Sea, near Zhongsha islands IDSSE-EEB-SW0082 OK044301 OL712214
Asteroschema sp. South China Sea, near Zhongsha islands IDSSE-EEB-SW0092 OK044302 OL712215
Asteroschema sp.SO2392113 Pacific Ocean: Clarion Clapperton Fracture Zone SO2392113 MN088049 -
Asteroschema clavigerum North Atlantic seamounts - HM587852 -
Asteroschema sublaeve Canada: British Columbia RBCM EC00271 HM400328 -
Asteroschema ajax Australia: off Lord Howe Isl. MVF99759 AB758762 AB605078
Asteroschema oligactes Off Dominica MNHN OM62 AB758766 AB758483
Asteroschema edmondsoni Off Santa Isabel Isl., New Caledonia MNHN OM13B AB758831 AB758486
Asteroschema horridum Off Reunion Isl. MNHN OM126 AB758764 AB758487
Asteroschema migrator Off Santa Isabel Isl., New Caledonia MNHN OM3 AB758765 AB758485
Ophiocreas ambonesicum Off Amami-oshima Isl., Kagoshima, Japan NSMT E-6502 AB758813 AB605084
Ophiocreas spinulosus Caribbean Sea, USA NMNH OM43 AB758820 AB758490
Ophiocreas glutinosum Off Katsuura, Chiba, Japan NSMT E-6710 AB758815 AB605086
Ophiocreas japonicus New Zealand NIWA T2494 AB758816 AB758488
Ophiocreas sibogae South Norfolk Ridge, New Zealand MV F99763 AB758818 AB605087
Ophiocreas caudatus Sagami Sea, Japan NSMT E-6259 AB758814 AB605085
Ophiocreas oedipus Off Hachijo-jima Isl., Ogasawara, Japan NSMT E-6375 AB758817 AB758489
Asterostegus maini South China Sea, near Xisha islands IDSSE-EEB-SW0076 OK044303 -
Asterostegus tuberculatus Western coast of Madagascar SMNH-123461 AB758769 AB758515
Asterostegus sabineae Madagascar: Off Reunion Island. SMNH-Type-8333 AB758768 AB758511
Astrodendrum sagaminum Japan: Sagami Sea NSMT E-5645 AB758795 -
Astrodendrum cf. sagaminum South China Sea, near Zhongsha islands IDSSE-EEB-SW0104 OK044304 -
Gorgonocephalus pustulatum New Zealand MVF188859 KU895114 -
Gorgonocephalus sundanus Australia MVF162682 KU895115 -
Gorgonocephalus arcticus Canada: Nunavut, Barrow Strait HLC-30309 HM543017 -
Gorgonocephalus caputmedusae Sweden: Skagerrak Echin 6305V MG935270 -
Gorgonocephalus eucnemis Japan: Iwate, Off Miyako NSMT E-5640 AB758809 -
Gorgonocephalus tuberosus Antarctic Sea. NIWA 38224 AB758811 -
Gorgonocephalus chilensis Antarctic Sea NIWA 38714 AB758812 -
Astrogymnotes irimurai Seseko Beach, Okinawa, Japan NSMT E-6716 AB758829 AB605123
Ophiomyxa anisacantha Sagami Sea, Japan NSMT E-6269 AB758822 AB605124

We constructed two maximum likelihood (ML) phylogenetic trees to represent the families Euryalidae and Gorgonocephalidae. Family Euryalidae: to construct the ML tree, we used 12 COI and nine 16S sequences from our collection and additionally 22 COI and 14 16S sequences from GenBank (Table 1). To construct the ML tree for the family Gorgonocephalidae, we used one species from our collection and an additional eight COI sequences from GenBank (Table 1). As outgroup we used COI and 16S sequences of Astrogymnotes irimurai Baker et al., 2001 and Ophiomyxa anisacantha H. L. Clark, 1911 for the ML trees.

All sequences were aligned using the ClustalW algorithm in MEGA X. When constructing the Euryalidae ML tree, we used the concatenated sequence alignment function in MEGA X to input both COI and 16S sequences. The best-fit substitution model of the COI and 16S gene in the ML trees was the General Time Reversible + Gamma Distributed (GTR + G) model, estimated by the “Find Best DNA/Protein Models” Option of MEGA X. Phylogenetic trees were reconstructed using the maximum likelihood bootstrap method. ML analysis was run with MEGA X, and ML trees were constructed, including 1,000 bootstrap replicates (Kimura 1980; Thompson et al. 1994; Kumar et al. 2016, 2018). The genetic distances were analyzed according to the Kimura 2-parameter model (Kimura 1980), and the standard error of each group was discovered by performing 1,000 bootstrap replications.

The following abbreviations are used in the text, tables, and figures

ap articular pad of the base;

ars/ARS arm spine;

arsb arm spine base;

as adoral shield;

asa arm spine articulation;

ass/ASS adoral shield spine;

au auricle;

AUS Australia;

CAN Canada;

co/CO conical ossicle;

COI Cytochrome C oxide subunit I;

CS Caribbean Sea;

d dorsal;

de depression;

dist distal;

fo foramina of the base;

fs fossa between adjacent tubercles;

go/GO granular ossicle;

goc granular ossicles coat;

gs/GS genital slit;

hd head of the apophysis;

iars inner arm spine;

IDSSE Institute of Deep-sea Science and Engineering;

irp interradial plate;

j jaw;

JAP Japan;

lac lateral ambulacral canal;

MAD Madagascar;

lap lateral arm plate;

ML Maximum Likelihood;

mo muscle opening;

mp median plate;

msv manned submersible vehicle;

NAT North Atlantic;

no nerve opening;

NWP North-West Pacific;

NZ New Zealand;

oars outer arm spine;

ob oral bridge;

os oral shield;

PAO Pacific Ocean;

pb podial basin;

pd pedicel of the apophysis;

peb pedicellarial band;

po/PO plate-like ossicle;

prox proximal;

pt primary tooth of the blade;

rs/RS radial shield;

SCS South China Sea;

sh sheath of the base;

st secondary tooth;

su sulcus of tubercle head;

t teeth;

TEP terminal projection;

tp tentacle pore;

v ventral.

Results

Seven species of Asteroschema were identified, among them two new to science that are described below. One species of each of the genera Asterostegus and Astrodendrum were identified, both of them are new to the South China Sea and described below. A tabular key to all species of Asteroschema is provided in Table 2. ML phylogenetic trees are presented in Figs 2 and 3, and genetic distances in Suppl. material 2: Tables S2 and Suppl. material 3: Table S3 of most of the species described in the study.

Table 2.

Tabular key to the species of Asteroschema and Ophiocreas. Abbreviations: ASS arm segment, ARS arm spine, GO granular ossicles, RS radial shield, CO conical ossicles, PO plate-like ossicles, TP terminal projection, GS genital slits.

Species Disc diameter and arm length Epidermal ossicles on the disc Epidermal ossicles on the arm ARS length ARS shape AS from segment 1st (2nd) Reference
Dorsal Ventral Dorsal Ventral
Asteroschema ajax A. H. Clark, 1949 13 mm and 300 mm fine GO; RS narrow, parallel, raised above the disc not meeting at center fine GO well-spaced annular bands when dried, covered with GO covered with GO as dorsal unknown unknown unknown Clark A. H. (1949)
Asteroschema arenosum Lyman, 1878 8–9 mm and arm length unknown (arm width 4 mm) coarse GO, 5 grains in 1 mm; RS wide, not meeting at center coarse GO but near mouth area scattered, jaw covered with GO coarse GO but denser than on disc as dorsal inner ≈ 2 × outer inner spine: cylindrical, slightly swollen, thorny dark tip 2 (4) Lyman (1878, H. L. Clark (1941), Pawson et al. (2009)
Asteroschema bidwillae McKnight, 2000 5 mm and 50 mm (6 arms) flat, small, very finely rugose GO, dense at center; RS and interradially (8–10 grains in 1 mm) but slightly spaced beside each RS; more or less extending to center very small well-spaced GO, except on elongated oral plates flat, small, very finely rugose GO, slightly spaced toward distal end, almost absent near tip, lateral surface always spaced GO present on plates near arm base, then naked inner > outer (inner 1 × ASE, outer 1/3 × ASE length) inner spine: slightly flattened, denticulate over most of length, and proximal margin beset with small curved spines 2 (8–14) McKnight (2000), Mah et al. (2009), this study
outer spine: small and inconspicuous distally both spines flattened and pointed, but hooks absent
Asteroschema brachiatum Lyman, 1879 6–11 mm and 270 mm (arm base width 3 mm) dense, uniform GO; RS elevated, extending nearly to center similar to dorsal, inconspicuous GO, which simulate oral papillae closely uniformly covered with GO; 6–9 grains in 1 mm as dorsal inner > outer (inner spine 2 mm) inner spine: rough, slightly club-shaped 2 (4) Lyman (1879, 1882), H. L. Clark (1941)
Asteroschema clavigerum Verrill, 1894 8–12 mm and arm length unknown (arm base width 3–3.5 mm) small, smooth GO, 6 grains in 1 mm; RS large, extending to center, GO larger than on disc. smooth skin lacking GO or minute, more spaced GO small, smooth GO only base of arm covered with minute, more spaced GO, and rest of arm naked inner > outer inner spine: large, long, elevated, and rough with spinules distally; somewhat swollen 3–4 (4–5) Verrill (1894), Döderlein (1927)
outer spine: small
distally both spines small, slender, acute and nearly equal
Asteroschema domogranulatum sp. nov. 9 mm and 165 mm dense, large slightly domed GO, 4 or 5 grains in 1 mm; RS wide, parallel, raised above the disc close together large polygonal PO except distal half of jaw large polygonal PO in proximal arm; then slightly separated, decreasing in size GO arm base concealed by polygonal PO; remainder naked inner ≈ 2 × outer inner spine: pointed thorny tip to cylindrical, slightly club shaped, flattened thorny 2 (19–22) This study
outer spine: small with thorny tip
distally both compound hook with 3–6 secondary teeth
Asteroschema edmondsoni A. H. Clark, 1949 13 mm and 290 mm GO & CO; dense GO on proximal half of RS, larger CO with TP on distal half dense rounded GO dense PO, swollen in the middle with TP; TP absent in distal end of arm same as on ventral disc; but smaller unknown unknown unknown Clark A. H. (1949)
Asteroschema elongatum Koehler, 1914 7–11 mm and 300 mm strongly excavated, GO rounded, unequal, slightly coarse near disc periphery; RS meeting at center, separated, GO larger and denser than on disc minute GO, uniform, separated, slightly stronger near periphery on first few segments GO like on RS then spaced, small, and uniform GO much smaller and uniform, rapidly becoming fewer and finally disappearing after 30–40 mm from arm base inner > outer inner spine: cylindrical, strong, thinner at tip with TP 2 (5) Koehler (1914), Döderlein (1930)
outer spine: conical, pointed tip
Asteroschema fastosum Koehler, 1904 6–13 mm and 180–300 mm (arm base width 5 mm) high, CO including RS small CO, more rounded, close-set at disc margin small CO, more close-set than on disc, 6 or 7 grains in 1 mm flattened CO inner > outer (inner spine ≤ 4 mm) inner spine: bluntly conical at arm base, then strongly club-shaped with TP 2 (4–5) Koehler (1904), Döderlein (1927, 1930), Guile (1981)
outer spine: cylindrical, thorny tip
distally both spines compound hook with 2–4 secondary teeth
Asteroschema flosculus Alcock, 1894 GO & CO; scattered as uniform microscopic GO uniform microscopic GO GO & CO; scattered as uniform microscopic GO uniform microscopic GO large unknown 3 (3) Alcock (1894)
Asteroschema glaucum Matsumoto, 1915 11 mm and 100 mm (4 mm width in arm base) flat; coarser GO, 6 grains in 1 mm; RS mostly covered except distal end coarser GO; near apex of jaw less GO coarser GO, 6 grains in 1 mm, very stout at base, as high as wide as dorsal inner > outer inner spine: cylindrical, club-shaped, rough end 2–3 (8) Matsumoto (1915)
distally both spines compound hook with four curved secondary teeth
Asteroschema hemigymnum Matsumoto, 1915 10 mm and 100 mm (3 mm width in arm base) very fine, smooth, close-set GO ventral: fine, rather sparse GO in skin, coarse, flat, smooth, pavement-like grains, corresponding to oral papillae very fine, smooth, close-set GO; 5 grains in 1 mm; GO much finer distalwards and disappear at distal end. entirely naked; LAP and VAP visible through skin inner > outer (outer spine ½ × ASE length) inner spine: cylindrical, club-shaped 2 (5–6) Matsumoto (1915)
outer spine: small, cylindrical, enclosed in skin, more or less rough tip
distally both spines compound hook with 3–6 secondary teeth
Asteroschema horridum Lyman, 1879 10–12.5 mm and 160–190 mm tumid polygonal PO; mostly tall CO with terminal projections PO & CO higher and thinner at disc periphery PO, larger on arm base than on disc, 4 PO in 1 mm at arm base; weakly annulated PO & CO lower and thinner inner ≈ 2 × outer inner spine: cylindrical with TP, slightly swollen 1 (1–6) Lyman (1879, 1882), Baker (1980), Mah et al. (2009), McKnight (2000)
Asteroschema igloo Baker, 1980 5.7 mm and 68.4 mm (1: 12-disc diameter to arm length) rounded or polygonal domed GO, 4–6 grains in 1 mm length; RS short, obscured, distally visible closely packed rounded, domed GO dorsal & ventral: rounded or polygonal domed GO; 4–6 grains in 1 mm length as dorsal inner ≈ 2 × outer (inner spine 2/3 × arm width) inner spine: long, very fine, thorny blunt tip 3: (8–10) Baker (1980), McKnight (2000)
distally both spines compound hook with 3–6 secondary teeth
Asteroschema inoratum Koehler, 1906 6–10 mm and 70+ mm fine, contiguous GO (rounded or slightly conical); RS wider, extending to center GO density similar to dorsal, slightly developed around GS fine, rounded or slightly conical, contiguous GO as dorsal inner ≈ 1½ × ASE length inner spine: slightly club-shaped, Swollen toward the end with conical point 2 (5–7) Koehler (1906), Peterson (1985), Hansson (2001), Smirnov et al. (2014)
outer spine: smooth, much smaller distally both spines small, but not transforming into a hook
Asteroschema intectum Lyman, 1878 5–11.5 mm and 280 mm (arm width 3 mm) fine GO, 6–7 grains in 1 mm; RS long, meeting at center, GO fine than on disc, 8–9 grains in 1 mm fine GO except jaw GO scattered, and smaller than on disc. lateral and ventral side naked or fewer GO inner > outer inner spine: blunt, spiniform, not club-shaped 2 (3) Lyman (1878), H. L. Clark (1941)
Asteroschema laeve (Lyman, 1872) 8.5 mm and 85 mm flat; fine close-set GO, 7–8 grains in 1 mm; RS mostly covered with GO except distal end minute, close, smooth GO, fewer GO near apex of jaw fine close-set GO, 7–8 grains in 1 mm, thin skin, faint brown marking as dorsal inner > outer inner spine: cylindrical, somewhat swollen, rough end 2 (8) Lyman (1875, 1879), H. L. Clark (1941)
distally both spines compound hook with 4 curved secondary teeth
Asteroschema lissum H. L. Clark, 1939 7.5 mm and 110 mm very fine, small GO; 50–60 grains in 1 mm2, but noticeably smaller at center and proximal end of RS; RS separated, narrow, straight, parallel, not meeting at center naked with thin skin fine GO similar on disc, laterally sparse, but continuous to base of ARS, distally sparse, and naked naked with thin skin inner ≈ 2–3 × outer inner spine: long, thick, thorny tip 2 (9–11) H. L. Clark (1939) This study
Asteroschema migrator Koehler, 1904 11 mm and 200–300 mm sparse, domed CO with terminal projections, CO dense on RS & disc margin few small CO close-set, small, tumid GO few GO or CO inner ≈ 2 × outer inner spine: cylindrical, swollen, TP outer spine: small, with pointed tip 3 (6–8) Koehler (1904) Baker (1980) McKnight (2000)
Asteroschema monobactrum H. L. Clark, 1917 8 mm and 80–90 mm (base arm width 2 mm) GO, flat, slightly raised above arm, near center 7 grains in 1 mm (50 mm2), but disc periphery 5–6 grains 1 mm (30 mm2)); RS completely covered but rounded ridges appeared when dried similar GO density as dorsal GO on arm base similar to disc, then separated, very minute, distally almost naked GO, from middle slightly naked inner ≈ 2 × outer unknown 2 (11–16) H. L. Clark (1917)
Asteroschema nuttingii Verrill, 1899 7 mm and 50 mm minute rough GO/CO; close-set on RS few GO near GS; minute rough GO/CO minute GO/CO, distinct distally minute rough GO/CO inner > outer inner spine: slender, tapering at arm base, then cylindrical, blunt, swollen distally, with TP 1 (1–2) Verrill (1899)
Asteroschema oligactes (Pallas, 1788) 4–10 mm and 250 mm (length ≈ 17 × disc diameter) CO CO CO; 4–5 CO in 1 mm on ventral arm base as dorsal inner > outer unknown unknown Pallas (1788), H. L. Clark (1941)
Asteroschema rubrum Lyman, 1879 12 mm and 160 mm fine, close-set GO, 6–7 grains in 1 mm; RS faintly indicated as flat ridges fine, close-set GO fine, close-set GO, 6–7 grains in 1 mm as dorsal inner spine maximum length 1.4 mm inner spine: small spiniform at arm base, then cylindrical, swollen with TP 2 (5–6) Lyman (1879, 1882), This study
distally both spines compound hook with secondary teeth
Asteroschema salix Lyman, 1879 5–8.5 mm and 55–85 mm flat, fine, close-set GO, 7–8 grains in 1 mm; RS mostly covered with GO except distal end fine, minute, close-set GO, less GO near apex of jaw fine, close-set GO, 7–8 grains in 1 mm, thin skin as dorsal inner > outer inner spine: cylindrical, somewhat swollen, rough end 2–3 (11–12) Lyman (1879), Baker (1980), McKnight (2000), Olbers et al. (2015), This study
distally both spines compound hook with 4 curved secondary teeth
Asteroschema sampadae Parameswaran & Jaleel, 2012 18 mm and 380–450 mm spaced CO with terminal projections; RS covered with CO, extending to center minute, spaced GO spaced CO with terminal projections, denser at arm base minute, spaced GO inner ≈ 2 × outer, inner spine ≤ 2 × ASE (5 mm) inner spine: bluntly conical at arm base; then cylindrical, with TP at inner edge 2 (4) Parameswaran and Jaleel (2012)
Asteroschema shenhaiyongshii sp. nov. 10 mm and 220 mm (arm base width 3.4 mm) small, finely rugose, rounded GO, similar in size, 8–9 grains in 1 mm; RS wide, parallel, close together, not meeting at center, distal end of RS raised above disc, swollen at center GO similar on dorsal, spearhead-shaped teeth. GS narrow concealed with GO dorsal: dense GO similar to disc; 8–9 grains in 1 mm; distally GO less rounded but dense less rounded and more polygonal GO, concealing only proximal half of arm, in middle to distal ventral arm surface concealed with widely separated GO decreasing in size to completely naked inner > outer (inner spine 2.2 mm long) inner spine: cylindrical, thorny tip to less club-shaped with small sharp thorns on more than half its length 2 (9–11) This study
outer spine: small in size with thorny tip similar to inner spine
distally both spines similar in size, compound hook with 4 or 5 secondary teeth
Asteroschema subfastostum Döderlein, 1930 8 mm and 9 × disc diameter pointed CO, blunt at disc margin, 4–5 grains in 1 mm smooth hemispherical GO pointed CO smooth hemispherical GO inner > outer unknown unknown Döderlein (1930)
Asteroschema sublaeve Lütken & Mortensen, 1889 12 mm and 300 mm (arm base width 5 mm) round, rugose GO variable size; RS covered with larger GO than on the disc smooth, small GO, few GO lateral at jaw round, rugose GO variable size, smaller at lateral side, larger on dorsal surface naked inner ≈ 2 × outer; in middle (inner ≈ 4 × outer) inner spine: elongated, club shaped, enclosed with thick skin 2–3 (3–4) Lütken and Mortensen (1889)
Asteroschema sulcatum Ljungman, 1872 5 mm and arm length unknown dense, small GO (9–15 grains in 1 mm); RS narrow, not meeting at center dense, small GO, teeth rounded or distally lobed dense, small GO as dorsal inner > outer inner spine: strongly thorny tip, swollen, bent club shaped (3–12) Ljungman (1872), Lyman (1875)
Asteroschema tenue Lyman, 1875 6 mm and 200 mm (arm base width 1.5 mm) closely, smooth GO (8–9 grains in 1 mm); RS narrow, meeting at center and GO little coarser coarser GO; large GO in jaw slender arms; similar to dorsal disc, distally GO much finer and more scattered as dorsal inner > outer, inner spine 1 mm long in middle half inner spine: spiniform at arm base, then large, fine thorny, club-shaped 1 (3) Lyman (1875), H. L. Clark (1915)
Asteroschema tubiferum Matsumoto, 1911 14–16 mm and 230–300 mm closely and evenly, small rounded or polygonal GO; RS narrow, not meeting at center but convergent entirely covered with dense GO, slightly large, rounded GO corresponding to oral papillae GO similar to disc, 4–5 grains in 1 mm distally GO smaller, widely spaced on ventral side inner > outer inner spine: cylindrical, initially tapering to a blunt, thorny tip, middle club-shaped with small sharp thorns, first 10–12 covered by sheath 2 (7) Matsumoto (1911, 1915), Baker (1980), McKnight (2000), This study
outer spine: small, pointed tip
distally both spines compound hook with secondary teeth
Asteroschema tumidum Lyman, 1879 8–13 mm and 120–180 mm dense, rounded GO proximally, CO at disc margin regularly spaced pointed; RS covered with CO, extending to center similar to dorsal but lower regularly spaced pointed, CO; 4 grains in 1 mm, rarely touching each other as dorsal inner > outer, inner spine ≤2 mm long inner spine: rough, slightly club-shaped 2 (3) Lyman (1879, 1882) Koehler (1904)
Asteroschema vicinum Koehler, 1907 7 mm and 93 mm fine GO fine GO, more than 9 grains in 1 mm, GO much larger around jaw fine GO as dorsal ½ × ASE length both sub-equal, fairly short, same morphology along the arm - (2) Koehler (1907)
Asteroschema wrighti McKnight, 2000 6.5 mm and at least 6 × disc diameter fine and uniform GO, 8–10 grains in 1 mm; RS elongated, meeting at center thin smooth skin, occasional small GO; relatively large irregular PO in GS fine and uniform GO; 8–0 grains in 1 mm; extending to lateral surface occasional small GO, thin and smooth skin inner > outer, inner arm spine up to 2 × ASE length inner spine: long, finely thorny, slightly club-shaped 2(5–6) McKnight (2000)
outer spine: relatively small and smooth
distally both spines compound hook with secondary teeth
Asteroschema yaeyamense Murakami, 1944 7 mm and 150 mm dense, coarse CO rounded GO regularly spaced CO, distally swollen GO as dorsal inner > outer inner spine: long, cylindrical with TP 2 (3–6) Murakami (1944), Liao (2004)
Ophiocreas ambonesicum Döderlein, 1927 27–30 mm and 350–390 mm coarse, thick, naked skin (when dry widely separated GO visible); RS narrow, not meeting at center as dorsal naked skin, when dry widely separated GO visible), annular band naked skin, when dry widely separated GO visible) inner ≈ 3 × outer; in middle 2 × ASS length inner spine: elongated, thick, enclosed with thick skin, cylindrical, club-shaped 2 (8–12) Döderlein (1927)
outer spine: cylindrical, pointed tip
distally both spines compound hook with 2–3 secondary teeth
Ophiocreas carnosus Lyman, 1879 15 mm and 200 mm (arm base width 7 mm) thick, soft wrinkled skin; RS rounded distal end, narrow, meeting at disc center as dorsal smooth, soft wrinkled skin as dorsal inner ≈ outer, inner ≈ 3 mm long inner spine: short, enclosed by thick skin, cylindrical, thorny tip 2 (6) Lyman (1879)
outer spine: cylindrical, thorny tip
Ophiocreas caudatus Lyman, 1879 22–25 mm and 300–420 mm (arm base width 5.5–7.5 mm) covered with thick skin, when dry micro-GO visible; RS narrow, raised above the disc, meeting at center as dorsal covered by thick skin, when dry micro-GO visible at arm base, annular band. as dorsal inner 3 mm longer in middle inner spine: elongated, enclosed by skin, stout, thorny tip 2 (10–13) Lyman (1879, 1882), Matsumoto (1917), Döderlein (1911), H. L. Clark (1949)
outer spine: short, peg-like
Ophiocreas oedipus Lyman, 1879 5–12 mm and 70–250 mm (arm base width 3.5 mm) thin skin with small, fine GO; RS narrow, closer together, extending to disc center small, closely set, rounded GO or naked, GS wide thin skin with fine GO, first 5–8 ASS swollen as dorsal inner 1 × ASS length, outer ½ × ASS length inner spine: slender, elongated, enclosed by skin, blunt, thorny tip 2 (6–9) Koehler (1904, 1909), Lyman (1879, 1882), H. L. Clark (1915), Baker (1980), Peterson (1985), McKnight (2000)
outer spine: short, cylindrical, pointed
distally both spines compound hook with 6 secondary teeth
Ophiocreas gilolense (Döderlein, 1927) 22 mm and 290 mm (arm base width 9 mm) naked skin; RS meeting at center as dorsal naked skin, annular band as dorsal inner ≈ 2 × outer; in middle 2 × ASS length inner spine: elongated, slender, cylindrical 2 (3–4) Döderlein (1927)
outer spine: cylindrical, pointed tip
Ophiocreas glutinosum (Döderlein, 1911) 17 mm and unknown (arm base height 9 mm) dense small GO (10 grains in 1 mm); RS large (nearly covering whole disc), long, close to each other, meeting at center as dorsal thick arms, thick skin covers arm plates completely, proximally dense small GO (10 grains in 1 mm), distally separated, as dorsal inner 1½ × ASS length, outer ≈ 1/3 × inner inner spine: slender, elongated, swollen thorny tip 2 (6–7) Döderlein (1911)
outer spine: small
distally both spines compound hook with 2–3 secondary teeth
Ophiocreas japonicus Koehler, 1907 4–33 and 210–655 mm (arm base width 7 mm) smooth, thin, small specimen with dense GO coverage (4 mm disc diameter); RS thick, raised above the disc, meeting at disc center as dorsal smooth, thin, naked skin, annular, (small specimen with dense GO coverage (4 mm disc diameter) somewhat fewer scattered GO inner ≤6 mm, 3 × ASS length; outer 1 × ASS length inner spine: elongated, thick base, cylindrical, thorny tip 2 (3–12) Koehler (1907), Döderlein (1911), Matsumoto (1917), McKnight (2000)
outer spine: cylindrical, pointed tip
distally both spines compound hook with 2–3 secondary teeth
Ophiocreas lumbricus Lyman, 1869 4.5–17 mm and 50–240 mm (arm base width 2.5 mm) covered by separated micro-thorny GO; RS meeting at center as dorsal covered by separated micro-thorny GO annular band as dorsal inner ≤ 1½–2 × ASS length outer 1 × ASS length inner spine: cylindrical, enclosed by skin, blunt, rough surface, thorny tip 2 (4) Lyman (1869)
outer spine: cylindrical, enclosed with skin, blunt, rough surface, thorny tip
Ophiocreas mindorense (Döderlein, 1927) 12–23 mm and 160–480 mm (arm base width 3–5.5 mm) smooth, dense GO (8 grains in 1 mm); RS narrow, closer together, extending to disc center as dorsal similar to disc, smooth, dense GO (8 grains in 1 mm); GO absent on ventral side of younger specimens as dorsal inner 3–5.5 mm, in middle 2½ –3 × ASS length; outer 1 × ASS length inner spine: slender, elongated, swollen thorny tip 2 (8–11) Döderlein (1927)
outer spine: thick arm base with pointed tip
Ophiocreas mortenseni Koehler, 1930 7.5–25 mm and 110–400+ mm (arm base height 6 mm) covered by thick, wrinkled, or folded skin; RS narrow, extending to disc center covered by plate-like ossicles skin thicker than dorsal, mostly thickened near arm spines, arched as dorsal inner 1 × ASS length; outer 2/3 × inner inner spine: slightly flattened, rough in upper half, thorny tip 2 (4–7) Koehler (1930), McKnight (2000)
outer spine: short, cylindrical, thorny pointed
distally both spines compound hook with 3 or 4 secondary teeth
Ophiocreas sibogae Koehler, 1904 14–28 mm and 300–350 mm (disc diameter × 30) (arm base width 5 mm) naked skin; RS narrow, parallel, not meeting at center as dorsal naked skin, annular band as dorsal inner ≈ 2 × outer, in middle 2 × ASS length inner spine: elongated, slender, cylindrical, club-shaped, finely rugose 2 (3–11) Koehler 1904, H.L. Clark 1916b, Mortensen 1924, Döderlein 1927, Baker 1980, McKnight (2000)
outer spine: cylindrical, pointed tip
distally both spines compound hook with 2–4 secondary teeth
Ophiocreas spinulosus Lyman, 1883 8–17 mm and 60–550 mm (arm base width 3 mm) naked skin; RS strongly marked ridges with short, stout blunt spines, meeting at center as dorsal higher than wide, naked skin, annular band blunt spine at each pair of ASS as dorsal inner ≈ 2 mm, equal in size in proximal arms inner spine: short, blunt, rough surface, cylindrical 2 (3–4) Lyman (1883), H. L. Clark (1915, 1941)
outer spine: cylindrical, pointed tip, distally slender
distally both spines compound hook with 2 secondary teeth
Ophiocreas willsi McKnight, 2000 16 mm and 380 mm covered by thick, wrinkled, or folded skin; RS narrow, extending to disc center, dense small GO cover in the center covered by well separated small GO. granulation similar to the disc, extending to the lateral arm, GO coverage dense proximally, but scattered distally first 3–5 ASS with few GO, then naked inner 1½ × ASS, outer shorter than ASS inner spine: slightly flattened, enclosed by skin, rough, blunt tip 2 (2–3) McKnight (2000)
outer spine: short, cylindrical, thorny pointed
distally both spines compound hook with 3 or 4 secondary teeth
Figure 2. 

Family Euryalidae, maximum likelihood (ML) tree based on partial COI and 16S sequences (bootstrap support values were generated with rapid bootstrapping algorithm for 1,000 replicates; blue = new species; brown = specimens from this study).

Molecular phylogenetic analysis

In total, 34 COI sequences trimmed to 592 bp and 25 16S sequences trimmed to 453 bp were obtained after removing ambiguous aligned sites and successfully reconstructing an ML tree for the studied Euryalidae (Fig. 2). Two main clades were detected within the ML tree of Euryalidae (clade 01: genus Asterostegus; clade 02: genera Asteroschema and Ophiocreas). Overall average genetic distances of COI between two clades were 10.04±1.34% SE (Asteroschema and Ophiocreas) and 3.08±0.75% SE (Asterostegus). The maximum value between two clades was 24.79%. Species from the genera Asteroschema and Ophiocreas separated into two subclades within main clade 02, but Asteroschema oligactes (Pallas, 1788), A. migrator Koehler, 1904, A. edmondsoni A. H. Clark, 1949, A. ajax A. H. Clark, 1949 and A. horridum Lyman, 1879 clustered with Ophiocreas species. Genetic distance between Asteroschema bidwillae and Asteroschema shenhaiyongshii sp. nov. was 2.59±0.67% SE (Suppl. material 2: Table S2).

A total of 11 COI sequences trimmed to 730 bp were obtained after removing ambiguous aligned sites, and successfully reconstructing an ML tree for the genera Gorgonocephalus and Astrodendrum (Fig. 3). Two clades were detected between the species. Clade 1 consists of Astrodendrum sagaminum (Döderlein, 1902), Gorgonocephalus pustulatum (H. L. Clark, 1916), and G. sundanus (Döderlein, 1927). Clade 2 consists of Gorgonocephalus arcticus Leach, 1819, G. eucnemis (Müller & Troschel, 1842), G. chilensis (Philippi, 1858), and G. tuberosus Döderlein, 1902. Overall average genetic distances of COI between two clades were 2.88±0.58% SE (clade 01) and 5.39±0.87% SE (clade 02). The maximum value between the two clades was 15.21%. Genetic distance between Astrodendrum sagaminum (AB758795) and Astrodendrum cf. sagaminum (OK044304) was 0.69±0.30% SE (Suppl. material 3: Table S3).

Figure 3. 

Family Gorgonocephalidae, maximum likelihood (ML) tree based on partial COI sequences (bootstrap support values were generated with rapid bootstrapping algorithm for 1,000 replicates; green = specimen from this study).

Taxonomic account

Class Ophiuroidea Gray, 1840

Superorder Euryophiurida O’Hara, Hugall, Thuy, Stöhr & Martynov, 2017

Order Euryalida Lamarck, 1816

Family Euryalidae Gray, 1840

Genus Asteroschema Örsted & Lütken in Lütken, 1856

Asteroschema domogranulatum sp. nov.

Figures 4, 5

Material examined

Holotype : China • 1 specimen; South China Sea, East of Zhongsha Islands, seamount; 16°22.11'N, 113°6.01'E; depth 1742 m; 09 Aug. 2020; Collecting event: stn. SC028; ‘Shenhaiyongshi’ msv leg; preserved in -80 °C; IDSSE-EEB-SW0089.

Paratypes : China • 2 specimens; same data as for holotype; IDSSE-EEB-SW0090, IDSSE-EEB-SW0091.

Diagnosis

Radial shields straight, parallel, close together, and raised above the disc and arms (Fig. 4A). Disc concealed by large polygonal, slightly domed granular ossicles (Fig. 4C). Jaws elongated, apex covered with few granular ossicles, but distal half naked. Ventral disc covered with large polygonal plate-like ossicles but naked around distal half of jaws (Fig. 4E). Dorsal and lateral surface of arms covered with plate-like or granular ossicles but dense only on few arms segments beyond the arm base (Fig. 4F–H). Ventral surface of the arm naked except arm base (Fig. 4I–L).

Figure 4. 

Asteroschema domogranulatum sp. nov., holotype (IDSSE-EEB-SW0089) A dorsal view B ventral view C dorsal disc D lateral disc E ventral disc F dorsal arm (proximal) G lateral arm (proximal) H lateral arm (distal) I ventral arm (base) J, K ventral arm (proximal) L ventral arm (middle) M, N arm spines (middle) O, P arm spines (distal). Abbreviations: ars arm spine, arsb arm spine base, as adoral shield, ass adoral shield spine, gs genital slit, iars inner arm spine, j jaw, oars outer arm spine, po plate-like ossicle, rs radial shields, t teeth, tp tentacle pore. Scale bars: 2 mm (A, B); 1 mm (C–G, K, L); 500 µm (H–J, M, N); 200 µm (O, P).

Description of holotype

Disc diameter 9 mm, length of arms 165 mm, arm base width 2.8–3.0 mm (Fig. 4).

Disc. Disc star-shaped, pentagonal, raised high above the arms, incised interradially and swollen on radial shields (Fig. 4A, B). Disc concealed by dense, large, polygonal, slightly domed ossicles (three or four grains in 1 mm; Fig. 4C). Radial shields bar-like, long, parallel, straight, adjacent pairs separated by narrow interradial disc, raised above the disc, and almost extending to center (Fig. 4C). Domed ossicles on distal half of radial shields larger (two or three grains in 1 mm) than in center (four grains in 1 mm; Fig. 4C). Genital slits narrow, vertical on interradii, dorsal half covered with ossicles similar to dorsal disc, ventral half similar to ventral disc (Fig. 4D). Jaws elongated, apex covered with few granular ossicles, but distal half naked (Fig. 4E). At apex of jaw a bluntly pointed tooth, at lateral edges a few granules that resemble lateral oral papillae (Fig. 4E). Ventral disc covered with large polygonal plate-like ossicles (three or four grains in 1 mm) except distal half of jaws (Fig. 4E). Adoral shields large but completely concealed by ossicles. Oral shields not discernible, and naked adoral shield spine (Fig. 4B, E).

Arms. Arms slender, arched at base, sub-cylindrical, increasingly cylindrical and narrower distalwards (Fig. 4F–H). Dorsal surface of arm base covered with large polygonal plate-like ossicles (three or four grains in 1 mm), then decreasing in size (five or six grains in 1 mm) and separated along the arm (Fig. 4F–H). Lateral plate covered with granular or plate-like ossicles, larger than on dorsal surface, and continuing to near base of arm spine (Fig. 4G, H). Distal half of arm laterally and dorsally covered with similar in size, separated granular ossicles (seven or eight grains in 1 mm; Fig. 4H). Ventral surface of arm base covered with polygonal plate-like ossicles (five or six grains in 1 mm), but after few arm segments from arm base completely naked (Fig. 4I–L). Tentacle pore at first arm segment without arm spine, but with small extended tube or sheath (Fig. 4I). Single arm spine from second arm segment with a second arm spine from nineteenth or twenty-second arm segment (Fig. 4I–L). Inner arm spine initially tapering to pointed thorny tip, middle half cylindrical, slightly club-shaped, one and a half arm segment in length, flattened, thorny (Fig. 4K–N). Outer arm spine half as long as inner spine in middle region, with thorny tip (Fig. 4N). Both arm spines similar in size at distal end, a compound hook with 3–6 secondary teeth (Fig. 4O, P).

Color. In live specimen, light brown color (Fig. 4).

Ossicle morphology of one paratype

IDSSE-EEB-SW090: Lateral arm plate curved around vertebrae, with strong curved rib with one arm spine articular structure, with single, completely separated large muscle and nerve openings (Fig. 5A). A depression on inner side of lateral arm plate (Fig. 5B). In proximal and middle half of arm inner arm spine slightly swollen, flat, and thorny on distal arm. Outer arm spine nearly half the size of the inner one with thorny tip (Fig. 5C). Distally, both spines changing into compound hook with secondary teeth (Fig. 5D). Arm concealed by polygonal large granular or plate-like ossicles (Fig. 5E). Vertebrae with streptospondylous articulation, with deep groove between proximal and distal end, dorsally a median longitudinal furrow, ventrally with deep median longitudinal groove containing lateral ambulacral canals, no oral bridge (Fig. 5F–J).

Figure 5. 

Asteroschema domogranulatum sp. nov., paratype (IDSSE-EEB-SW0090) A, B lateral arm plate (external, internal) C arm spines (middle) D arm spine (distal) E skin from dorsal arm base, insert frame shows polygonal plate-like large ossicle F–J vertebrae F proximal view G distal view H lateral view I dorsal view J ventral view. Abbreviations: d dorsal, de depression, dist distal, iars inner arm spine, lac lateral ambulacral canals, mo muscle opening, no nerve opening, oars outer arm spine, pb podial basin, prox proximal, st secondary teeth, v ventral. Scale bars: 800 µm (F–J); 500 µm (C); 300 µm (B, D, E); 200 µm (A).

Paratypes variations

Disc diameter 6.5 and 8 mm, and both basically identical to holotype. However, the segment at which the second arm spine first appeared varied (14–20 free segments), but is considered intraspecific variation.

Distribution and habitat

1742 m depth. Zhongsha Islands, the South China Sea. Attached to coral host.

Etymology

The species name is derived from the Latin words domus, meaning dome, and granulatus, meaning granulated, referring to the domed granular ossicles on the disc.

Remarks

The here examined new species was collected on a deep-sea seamount, attached to an unidentified coral species. It concurs with the group that has domed and plate-like granular ossicles, in the genus Asteroschema. This clade included only one species, prior to this study (Asteroschema igloo Baker, 1980). Large polygonal plate-like ossicles were the most significant morphological character for delimiting most of the other Asteroschema species from A. domogranulatum sp. nov. (Table 2).

Asteroschema domogranulatum sp. nov. strongly resembles A. igloo. They are similar in size according to McKnight’s (2000) description (8 mm disc diameter). Therefore, here we include a comprehensive morphological analysis to distinguish A. domogranulatum sp. nov. from A. igloo such as (see also Table 2): in A. domogranulatum sp. nov. radial shields raised above the arms and disc, straight, parallel, with narrow gap, whereas in A. igloo distal ends of radial shields much wider apart, converging to center, in A. domogranulatum sp. nov. polygonal granular ossicles on dorsal disc, in center smaller than at distal edge, but in A. igloo concealed by polygonal or rounded domed ossicles, and in center large, domed, rounded ossicles, in A. domogranulatum sp. nov. teeth pointed but in A. igloo ventralmost one pointed and others blunt spearhead-shaped, in A. domogranulatum sp. nov. ventral disc covered with polygonal plate-like ossicles, and distal half of jaw naked but in A. igloo completely covered with compact polygonal or rounded domed ossicles, in A. domogranulatum sp. nov. only dorsal and lateral surface covered with plate-like or granular ossicles, dense only on few arm segments from arm base, and naked ventral arm except arm base but in A. igloo whole arm covered with dense, rounded or polygonal domed ossicles, in A. domogranulatum sp. nov. inner arm spine slightly swollen, blunt, flattened, and outer arm spine with thorny pointed tip but in A. igloo inner arm spine swollen, blunt, and outer arm spine with smooth pointed tip, in A. domogranulatum sp. nov. start of first arm spine at second arm segment, and second arm spine at nineteenth or twenty-second arm segment but in A. igloo first arm spine from third arm segment, and second arm spine starts at eighth or tenth arm segment (McKnight 2000). The most significant morphological characters of A. domogranulatum sp. nov. were the appearance of the radial shields, and the granulation of ventral disc and arms (Fig. 4).

Asteroschema shenhaiyongshii sp. nov.

Figures 6, 7

Material examined

Holotype : China • 1 specimen; South China Sea, Northeast of Xisha Islands archipelago; 18°41.95'N, 113°33.08'E; depth 1070 m; 29 Mar. 2018; Collecting event: stn. SC004; ’Shenhaiyongshi’ msv leg; preserved in -80 °C; GenBank: OK044292, OL712208; IDSSE-EEB-SW0086.

Paratype : China • 1 specimen; South China Sea, Southeast of Zhongsha Islands; 13°55.30'N, 115°25.44'E; depth 1111 m; 04 Aug. 2020; Collecting event: stn. SC007; ‘Shenhaiyongshi’ msv leg; preserved in -80 °C; IDSSE-EEB-SW0087.

Diagnosis

Disc raised high above the arm, concealed by highly dense, small, rounded, finely rugose granular ossicles (Fig. 6A–C). Jaws narrow, elongated, concealed by slightly larger, less rounded granular ossicles (Fig. 6E). Arm surface concealed by granular ossicles similar to disc, but ventral surface of arm concealed by less rounded, more polygonal granular ossicles (Fig. 6F–H). Inner arm spine cylindrical, slightly club-shaped, with small sharp thorns on more than half its length (Fig. 6L–N).

Figure 6. 

Asteroschema shenhaiyongshii sp. nov., holotype (IDSSE-EEB-SW0086) A dorsal view B ventral view C dorsal disc D lateral disc E oral frame F dorsal arm (base) G lateral arm (proximal) H dorsal arm (distal) I ventral arm (base) J ventral arm (middle) K ventral arm (distal) L arm spine (arm base) M, N arm spines (middle) O, P arm spines (distal). Abbreviations: ars arm spine, go granular ossicle, goc granular ossicles coat, gs genital slit, iars inner arm spine, j jaw, oars outer arm spine, rs radial shield, t teeth, tp tentacle pore. Scale bars: 2 mm (A, B); 1 mm (C, D, F, I, J); 500 µm (E, G, H, K, M, N, P); 200 µm (L, O).

Description of holotype

Disc diameter 10 mm, length of arms 220 mm, arm base width 3.3–3.5 mm (Fig. 6).

Disc. Disc more or less pentagonal, raised high above arm, and swollen in center (Fig. 6A). Entire disc concealed by highly dense, small, rounded, finely rugose granular ossicles (eight or nine grains in 1 mm; Fig. 6A–C). Granular ossicles similar in size and shape from center to periphery of disc (Fig. 6C). Radial shields wide, straight, close together, but not meeting in center, and completely concealed by dense granulation (Fig. 6C). Genital slits narrow, vertical at interradii and densely covered with less rounded granular ossicles (Fig. 6D). Jaws narrow, elongated, concealed by slightly larger less rounded granular ossicles (Fig. 6E). At apex of jaw a blunt, spearhead-shaped tooth, and granular ossicles all over oral plates (Fig. 6E). Ventral disc densely covered with granular ossicles similar to dorsal disc (seven or nine grains in 1 mm) but less rounded around distal end of jaw and adoral shields (Fig. 6E). Adoral shields large, with curved edge, and connected to first arm segment. Oral shields not discernible and adoral shield spine covered by granular ossicles (Fig. 6E).

Arms. Arm width comparatively large in relation to body size, not arched, sub-cylindrical, width unchanged from base to middle half of arm (Fig. 6A, F). From middle to distal end, arm tapering slightly and more cylindrical (Fig. 6F–H). Dorsal and lateral arm surface concealed by dense, finely rugose, rounded granular ossicles similar to disc (eight or nine grains in 1 mm), continuing to distal end of arm (Fig. 6F–H). Distal half of dorsal and lateral arm concealed by less rounded, dense granular ossicles (eight or nine grains in 1 mm; Fig. 6H). Lateral arm plates on proximal to middle half of arm concealed by granular ossicles, including on base of arm spine, but on distal end only lateral arm plates concealed (Fig. 6G–K). Ventral surface of arm concealed by dense granular ossicles, similar to ventral disc, less rounded and more polygonal, but only covering proximal half of arm (seven or nine grains in 1 mm; Fig. 6I). Middle to distal end of ventral arm surface concealed by widely separated, in size decreasing granular ossicles (three or four grains in 1 mm) to completely naked (Fig. 6J, K). First free tentacle pore without arm spine (Fig. 6I). First arm spine appears at second arm segment, with short blunt tentacle scale. Second arm spine appears at nineth or eleventh segment (Fig. 6I, J). Inner arm spine initially tapered to pointed thorny tip, one arm segment in length, middle half cylindrical, less club-shaped, with small sharp thorns on more than half of spine length (Fig. 6L–N). Outer spine smaller, with thorny tip (Fig. 6M, N). Both arm spines similar in size at distal end of arm, and turning into compound hook with 4–5 secondary teeth (Fig. 6O, P).

Color. In live specimen, reddish brown (Fig. 6).

Ossicle morphology of paratype

Lateral arm plate curved around the vertebrae, with two arm spine articular structures, with completely separate large muscle and nerve openings (Fig. 7A). A depression on inner side of lateral arm plate (Fig. 5B, C). Inner arm spine becoming cylindrical from proximal to middle half of arm, with terminal projection, and thorny surface on upper part of spine (Fig. 7D). Outer arm spine nearly half as long as inner one with less thorny tip (Fig. 7E). Distally both spines compound hook with 3–5 secondary teeth (Fig. 7F, G). Arm and disc concealed by rounded granular ossicles (Fig. 7H). Vertebrae with streptospondylous articulation, with deep slope between proximal and distal end, dorsally a median longitudinal furrow, ventrally with median deep longitudinal groove with lateral ambulacral canals, no oral bridge, podial basins moderate in size (Fig. 7I–M).

Figure 7. 

Asteroschema shenhaiyongshii sp. nov., paratype (IDSSE-EEB-SW0087) A–C lateral arm plate (external, internal) D, E arm spines (middle) F, G arm spine (distal) H skin from dorsal arm base (rounded granular ossicles) I–M vertebrae I proximal view J distal view K lateral view L dorsal view M ventral view. Abbreviations: d dorsal, de depression, dist distal, lac lateral ambulacral canals, mo muscle opening, no nerve opening, pb podial basin, prox proximal, st secondary teeth, v ventral. Scale bars: 500 µm (D, I–M); 300 µm (A–C, E, G); 200 µm (F, H).

Paratype variations

Paratype disc diameter 12 mm, similar to holotype. Second arm spine from segments 10–12 and disc slightly flatter than those of holotype; considered intraspecific variation.

Distribution and habitat

1070–1111 m depth. Near Xisha and Zhongsha islands in the South China Sea. Attached to sponge host.

Etymology

The specific name is dedicated to the manned submersible vessel ‘Shenhaiyongshi’ meaning deep sea warrior in Chinese, which collected the specimen.

Remarks

Asteroschema shenhaiyongshii sp. nov. concurs with the group of Asteroschema that has only granular ossicles. This group includes nearly 20 species. Furthermore, they are divided by naked, widely separated granular ossicles on ventral disc and base of arm. Fifteen Asteroschema species have dense granular ossicles on the ventral disc. Asteroschema shenhaiyongshii sp. nov. is distinguished clearly by its unique dense, rounded, fine granular ossicles on both disc and arms, a thick sub-cylindrical proximal to middle half of the arms without annular bands, and cylindrical outer arm spine with visible thorns at middle arm segments (Fig. 7).

Asteroschema ajax and A. laeve (Lyman, 1872) are similar to A. shenhaiyongshii sp. nov. by having granular ossicles on both dorsal and ventral disc, but are easily differentiated by well-spaced annulated granular bands on the arms, and club-shaped inner arm spine (Clark A. H. 1949; Lyman 1875; FWRI 2010).

Asteroschema arenosum Lyman, 1878 is similar to A. shenhaiyongshii sp. nov. by having granular ossicles on both dorsal and ventral disc, but differs in having a swollen arm spine, sparser granular coverage (five grains in 1 mm), and a second arm spine from the fourth arm segment. Asteroschema vicinum Koehler, 1907 differs in its sub-equal arm spines and A. sulcatum Ljungman, 1872 in its highly dense granular ossicle coverage (9–15 grains in 1 mm). Asteroschema igloo and A. domogranulatum sp. nov. differ from A. shenhaiyongshii sp. nov. by large polygonal to domed granular ossicles (four or five grains in 1 mm; Table 2) (Ljungman 1872; Lyman 1878; Koehler 1907; Pawson et al. 2009).

Asteroschema glaucum Matsumoto, 1915, and A. hemigymnum Matsumoto, 1915 are similar to A. shenhaiyongshii sp. nov. by having granular ossicles on both dorsal and ventral disc, but differ by sparser granular ossicle coverage (five or six grains in 1 mm), club-shaped inner arm spine at the middle arm segments and in A. hemigymnum the ventral disc is covered with pavement-like ossicles. Asteroschema inoratum Koehler, 1906 is similar to A. shenhaiyongshii sp. nov. by having a similar density of the granular coverage on both dorsal and ventral disc, but differs in having granular ossicles on the ventral surface along the arm, a slightly club-shaped inner arm spine at middle arm segments, and both spines not transforming into a compound hook (Table 2) (Koehler 1906; Matsumoto 1915).

Asteroschema monobactrum H. L. Clark, 1917 is similar to A. shenhaiyongshii sp. nov. in having granular ossicles on both dorsal and ventral surface and in start of the second arm spine, but differs by having separate, sparser granular ossicles (seven grains in 1 mm at disc center, five or six grains in 1 mm at periphery of disc and on arm), and by the granular ossicles on both sides distalwards along the arm becoming widely separated to almost naked (H. L. Clark 1917). Asteroschema brachiatum Lyman, 1879 is similar to A. shenhaiyongshii sp. nov. in having similar density granular ossicle coverage on both dorsal and ventral disc and arms, but differs by the entire arm being covered by granular ossicles, start of the second arm spine at the fourth arm segment, and a slightly club-shaped inner spine at middle arm segments (Lyman 1879). Asteroschema salix Lyman, 1879, A. tubiferum Matsumoto, 1911, and A. rubrum Lyman, 1879 differ from A. shenhaiyongshii sp. nov. by having separated, sparser granular ossicle coverage, a club-shaped inner spine at middle arm segments, and widely spaced ossicles on the ventral arm surface (Lyman 1879; Matsumoto 1911).

Granular ossicle density of Asteroschema wrighti McKnight, 2000, A. bidwillae McKnight, 2000, and A. tenue (eight or ten grains in 1 mm) is similar to A. shenhaiyongshii sp. nov. However, Asteroschema tenue differs from A. shenhaiyongshii sp. nov. by having a club-shaped inner spine at middle arm segments, and slender long arms, and A. wrighti differs by having a club-shaped inner spine at middle arm segments, widely spaced granular ossicles on the ventral arm, irregular plate-like ossicles on the ventral disc, and smooth outer spines, and by being hexamerous (Table 2) (Lyman 1875; McKnight 2000).

Most Ophiocreas species differ from Asteroschema shenhaiyongshii sp. nov. by having naked or micro-granular ossicles in the skin in both disc and arms. (Table 2). In our phylogenetic tree of the family Euryalidae, all Ophiocreas species cluster with some Asteroschema species, but the average genetic distance between Ophiocreas species and Asteroschema shenhaiyongshii sp. nov. was 13.61% (Fig. 2, Suppl. material 2: Table S2). Asteroschema shenhaiyongshii sp. nov. clusters among Asteroschema rubrum, A. salix, A. tubiferum, A. cf. lissum, and A. bidwillae. All these Asteroschema species have granular ossicles on the dorsal disc and dorsal surface of the arms. Asteroschema bidwillae showed a close relationship with A. shenhaiyongshii sp. nov., and was identified as a sibling species due to similar morphological characters of granular ossicles on the dorsal disc and dorsal surface of the arms, and the shape of the inner arm spines at middle arm segments, but differs by having well-spaced granular ossicles on the ventral disc, a naked proximal ventral arm surface, and by being hexamerous and fissiparous (Table 2, Suppl. material 2: Table S2).

Asteroschema cf. bidwillae McKnight, 2000

Figures 8, 9

Asteroschema bidwillae McKnight, 2000: 24–27, fig. 8.

Material examined

China • 1 specimen; South China Sea, Zhongsha Islands, seamount; 13°36.20'N, 113°33.74'E; depth 1515 m; 30 Mar. 2020; Collecting event: stn. SC025; ‘Shenhaiyongshi’ msv leg; preserved in -80 °C; IDSSE-EEB-SW0105.

Description

Disc diameter 13 mm, length of arms 195 mm, arm base width 4.5–5 mm (Fig. 8).

Disc. Disc circular, hexamerous, raised above arms, deeply swollen in center (Fig. 8A, B). Disc covered with dense, small, finely rugose granular epidermal ossicles (Fig. 8A–C). Granular ossicles dense and small in size in disc center, but slightly larger at distal edge (six or seven grains in 1 mm; Fig. 8C, D). Radial shields extending to center but proximal ends concealed by skin with granular ossicles, and distal ends raised above the disc (Fig. 8C). Granular ossicles around distal edge and periphery of disc larger and more irregular (Fig. 8C). Genital slits narrow, without ossicles and vertical on ventral interradii (Fig. 8E). Jaws elongated, mostly naked without granular ossicles (Fig. 8F). At apex of jaw flattened, pointed, and finely rugose teeth, and two to four granular tubercles that resemble lateral oral papillae (Fig. 8F). Ventral disc covered with widely separated small granular ossicles (four or six grains in 1 mm; Fig. 8B, F). Adoral shields connected to first ventral arm segment and concealed by widely separated small granular ossicles, but outline of shields clearly visible (Fig. 8F). Oral shields not discernible and adoral shield spine covered by ossicles (Fig. 8F).

Figure 8. 

Asteroschema cf. bidwillae McKnight, 2000 (IDSSE-EEB-SW0105) A dorsal side of the specimen B ventral side of the specimen C dorsal disc D skin on the disc E lateral disc F oral frame G dorsal arms (proximal) H dorsal arm (middle) I ventral arm (arm base) J outer arm spine (proximal) K arm spines (middle) L arm spines (distal). Abbreviations: ars arm spine, as adoral shield, ass adoral shield spine, go granular ossicle, gs genital slit, iars inner arm spine, j jaw, oars outer arm spine, rs radial shield, t teeth, tp tentacle pore. Scale bars: 2 mm (A–C, E, G); 1 mm (F, H, I); 500 µm (K, L); 200 µm (D, G, J).

Arms. Arms six, at base wide, not arched, dorsally flattened, and swollen in first few free arm segment (Fig. 8G). Arms distalwards from middle part narrowing and increasingly cylindrical (Fig. 8H). Swollen dorsal arm base covered with dense, large, irregular granular ossicles (four or seven grains in 1 mm), distalwards decreasing in size and becoming rounded (six or seven grains in 1 mm), and distally widely separated (Fig. 8G, H). Lateral arm plates covered with slightly separated granular ossicles. Ventral arm near base covered with granular ossicles similar to ventral disc (five or six grains in 1 mm), but becoming widely separated to completely naked along the arm (Fig. 8I, J). First two or three arm segments without arm spine (Fig. 8I). First arm spine appeared at third or fourth arm segment, and second arm spine at eighteenth or twenty-first segment (Fig. 8I–K). Inner arm spine cylindrical, with blunt thorny tip, one and a half arm segment in length (Fig. 8J, K). Outer spine half as long as inner spine in middle region, with thorny, pointed tip (Fig. 8K). Both arm spines equal in length at distal end of arm, and compound hook with 3–6 secondary teeth (Fig. 8L).

Color. In live specimen, reddish brown (Fig. 8).

Ossicle morphology

Lateral arm plate with two arm spine articular structures, with slightly separated large muscle and relatively small nerve opening (Fig. 9A). A depression on inner side of lateral arm plate (Fig. 9B). Inner arm spine from proximal and middle half of arm with cylindrical, terminal projection, and upper part of spine covered with thorns (Fig. 9C). Distally, arm spine turns into compound hook with secondary teeth (Fig. 9D). Arm and disc concealed by rounded to slightly irregular granular ossicles (Fig. 9E). Vertebrae with streptospondylous articulation, dorsally a median large longitudinal furrow, ventrally with median deep groove with lateral ambulacral canals, podial basins small (Fig. 9F–J).

Figure 9. 

Asteroschema cf. bidwillae McKnight, 2000 (IDSSE-EEB-SW0105) A, B lateral arm plate (external, internal) C outer arm spine (middle) D arm spine (distal) E skin from dorsal arm base (rounded to irregular-shaped granular ossicles) F–J vertebrae F proximal view G distal view H lateral view I dorsal view J ventral view. Abbreviations: asa arm spine articulation, d dorsal, de depression, dist distal, lac lateral ambulacral canals, mo muscle opening, no nerve opening, pb podial basin, prox proximal, st secondary teeth, v ventral. Scale bars: 800 µm (F–J); 500 µm (C); 300 µm (A, B, E); 200 µm (D).

Distribution

400–2000 m depth. New Zealand, Tasman Sea, Solomon Islands, South China Sea (OBIS 2021).

Remarks

Asteroschema bidwillae was first described by McKnight (2000), with type locality New Zealand waters in the South Pacific Ocean. This is the first redescription since the original description. The specimens from our collection concur with McKnight’s description, but we noticed some differences such as: granular arrangement on radial shields, irregular ossicles on arm base, ossicles on ventral arm recorded nearly to middle region, and start of second arm spine. However, some of these variations may be related to size and maturity of the specimen (the holotype had a disc diameter of 5 mm). We hesitate to fully associate our specimen with Asteroschema bidwillae due to uncertainty with the morphological variation in A. bidwillae. The genus Asteroschema contains only two hexamerous species as far as known. Therefore, the closest one is Asteroschema wrighti McKnight, 2000, but it differs in characters of the radial shields, granulation on disc and arm, innermost arm spine, and start of second arm spine (Table 2). This is the first record of A. bidwillae from the North Pacific Ocean, if it is indeed this species.

Asteroschema rubrum Lyman, 1879

Figures 10, 11

Asteroschema rubrum Lyman, 1879: 68–69, fig. 17, figs 454–457.

Material examined

China • 3 specimens; South China Sea, near Zhongsha Islands, seamount; 13°55.44'N, 115°25.37'E; depth 958 m; 09 Mar. 2020; Collecting event: stn. SC007; ‘Shenhaiyongshi’ msv leg; preserved in -80 °C; GenBank: OK044293, OL712209, OK044294, OL712210, OK044295, OL712211; IDSSE-EEB-SW0071, IDSSE-EEB-SW0072, IDSSE-EEB-SW0073 • 1 specimen; South China Sea, near Zhongsha Islands, seamount; 14°21.93'N, 115°23.89'E; depth 922 m; 17 Mar. 2020; Collecting event: stn. SC035; ‘Shenhaiyongshi’ msv leg; preserved in -80 °C; IDSSE-EEB-SW0088.

Description

IDSSE-EEB-SW0072: disc diameter 12 mm, length of arms from 165–175 mm (Fig. 10).

Disc. Disc flat, slightly raised above arms, swollen in center, and small in relation to total body size of specimen (Fig. 10A, B). Disc covered with smooth, dense, evenly distributed, small rounded or irregular granular ossicles, similar in size (seven or eight granular ossicles in 1 mm; Fig. 10C, D). Radial shields closely together, parallel, raised above distal disc edge, but mostly concealed by skin with granular ossicles (Fig. 10C). Radial shields do not meet in center. Genital slits wide and vertical on ventral interradii (Fig. 10E). Jaw large, long and covered with dense irregular ossicles (Fig. 10F). Spearhead-shaped teeth and granular ossicles that resemble lateral oral papillae at apex of jaw (Fig. 10F). Adoral shields large, connected to first ventral arm segment, and concealed by granular ossicles (Fig. 10F). Oral shields not discernible and adoral shield spine naked. Whole oral region swollen nearly to genital slit. Ventral disc covered with dense granular ossicles (seven or eight grains in 1 mm; Fig. 10F).

Figure 10. 

Asteroschema rubrum Lyman, 1879 (IDSSE-EEB-SW0072) A dorsal view B ventral view C dorsal disc D skin on the disc E lateral disc F ventral disc G dorsal arm (proximal) H lateral arm (middle) I ventral arm (proximal) J outer arm spine (proximal) K arm spines (middle) L arm spines (distal). Abbreviations: as adoral shield, ass adoral shield spine, ars arm spine, go granular ossicle, gs genital slit, iars inner arm spine, j jaw, oars outer arm spine, ass adoral shield spine, rs radial shield, t teeth, tp tentacle pore. Scale bars: 2 mm (A, B); 1 mm (E–G, I); 500 µm (C, H, J, K); 200 µm (D, L).

Arms. Arms at base wide, not arched, dorsally flattened, and slightly swollen in first few free segments (Fig. 10A, G). Arms distalwards from middle part narrowing and more cylindrical (Fig. 10H). Dorsal arm base covered with smooth rounded granular ossicles (six or seven grains in 1 mm), middle segments with dense granular ossicles all the way to the arm spine base (seven or eight grains in 1 mm), and distally decreasing in size and separated (seven or eight grains in 1 mm) (Fig. 10G–L). Ventral arm base covered with dense granular ossicles similar to the ventral disc (eight or nine grains in 1 mm), distally decreasing in size and separated to naked (Fig. 10H–K). First one to two tentacle pores without arm spine (Fig. 10F). First arm spine appears at second or third arm segment, and second arm spine at ninth or eleventh segment (Fig. 10I). Outer arm spine half as long as inner spine in middle region, thorny pointed tip, distally compound hook (Fig. 10K, L). Inner arm spine cylindrical, one to one and a half arm segment in length, initially tapering to a pointed thorny tip, in middle blunt, slightly swollen with thorny surface on more than half its length, distally compound hook with three or four secondary teeth (Fig. 10I–L).

Color. In live specimen, reddish brown (Fig. 10).

Ossicle morphology

Lateral arm plate with two arm spine articular structures, with slightly separate large muscle and nerve openings (Fig. 11A). A depression on inner side of lateral arm plate (Fig. 11B). Inner arm spine from proximal and middle half of arm cylindrical, slightly swollen, with thorny tip (Fig. 11C). Distally arm spine turns into compound hook with secondary teeth (Fig. 11D). Arm and disc concealed by granular ossicles, slightly wider than high, round to short stumps with convex tip (Fig. 11E). Vertebrae with streptospondylous articulation, with deep slope between proximal and distal end, dorsally a median longitudinal groove, ventrally with median deep longitudinal groove with lateral ambulacral canals, podial basins moderate in size (Fig. 11F–J).

Figure 11. 

Asteroschema rubrum Lyman, 1879 (IDSSE-EEB-SW0072) A, B lateral arm plate (external, internal), C outer arm spine (middle), D arm spine (distal), E skin from dorsal arm base (rounded to somewhat cone shaped granular ossicles), F–J vertebrae; F proximal view, G distal view, H lateral view, I dorsal view, J ventral view. Abbreviations: d dorsal, de depression, dist distal, lac lateral ambulacral canals, mo muscle opening, no nerve opening, pb podial basin, prox proximal, st secondary teeth, v ventral. Scale bars: 800 µm (C); 500 µm (A, F, G); 300 µm (B, E, H–J); 200 µm (D).

Distribution

730–958 m depth. Near Brandella, Chile and in the South China Sea.

Remarks

Asteroschema rubrum was first described by Lyman (1879), with type locality in the Southwest Pacific Ocean near South America. This is the first rediscovery after the original description. The specimens from our collection concur well with Lyman’s holotype description, the only difference was the starting point of the second arm spines. However, this morphological character is highly variable among individuals. According to the holotype description, A. rubrum belongs in the clade with granular ossicles only in the genus Asteroschema, but Okanishi and Fujita (2009) considered A. rubrum in the clade with conical and granular ossicles. Although, the SEM images of granular ossicles in the skin appear as granular to somewhat small stumps with convex tip (Fig. 11E). However, the description of the holotype is identical with our specimen, and it was described as granular ossicles. The characters of the swollen oral region, smooth granulation on the disc, and innermost spine can be used to distinguish A. rubrum from other species of Asteroschema (Table 2). The dorsal disc of A. rubrum seems naked in wet condition due to its smooth granulation which can lead to misidentification as Ophiocreas species (Fig. 10A). This is the first record from the South China Sea.

Asteroschema tubiferum Matsumoto, 1911

Figures 12, 13

Asteroschema tubiferum Matsumoto, 1911: 52; 1917: 44; Baker 1980: 22, fig. 4; McKnight 2000: 24, fig. 7.

Material examined

China • 1 specimen; South China Sea, Zhongsha Islands, seamount; 13°36.20'N, 113°33.74'E; depth 1515 m; 30 Mar. 2020; Collecting event: stn. SC025; ‘Shenhaiyongshi’ msv leg; preserved in -80 °C; GenBank: OK044298; IDSSE-EEB-SW0077 • 1 specimen; South China Sea, East of Zhongsha Islands, seamount; 16°22.11'N, 116°06.60'E; depth 1619 m; 09 Aug. 2020; Collecting event: stn. SC028; ‘Shenhaiyongshi’ msv leg; preserved in -80 °C; GenBank: OK044297, OL712213; IDSSE-EEB-SW0106. Northwest Pacific • 1 specimen; near Mariana Trench, Southeast of Guam Island, deepsea seamount, 11°57.20'N, 141°28.67'E; depth 1377 m; 03 Sep. 2019; Collecting event: stn. SC034; ‘Shenhaiyongshi’ msv leg; preserved in -80 °C; GenBank: OK044296, OL712212; IDSSE-EEB-SW0078.

Description

IDSSE-EEB-SW0078: disc diameter 10 mm, length of arms 200 mm (Fig. 12).

Disc. Disc flat, slightly raised above arms, swollen in center (Fig. 12A, B). Disc covered with smooth, small, closely spaced, and evenly rounded or polygonal granular ossicles, dense in disc center (seven or eight grains in 1 mm), but larger and polygonal at distal edge (six or seven grains in 1 mm) (Fig. 12C, D). Radial shields not meeting in center, but converging (Fig. 12C). Distal end of radial shields raised above disc and wider than proximal end (Fig. 12C). Genital slits narrow, vertical on ventral interradii (Fig. 12E). Jaws elongated, covered densely with granular ossicles (Fig. 12F). Flattened, pointed, and spearhead-shaped teeth and granular ossicles that resemble lateral oral papillae at apex of jaw (Fig. 12F). Ventral disc densely covered with granular and polygonal ossicles (seven or eight grains in 1 mm; Fig. 12F). Adoral shields connected to first ventral arm segment and concealed by granular ossicles but outline of shields visible. Oral shields not discernible and adoral shield spine densely covered by ossicles (Fig. 12F).

Figure 12. 

Asteroschema tubiferum Matsumoto, 1911 (IDSSE-EEB-SW0078) A dorsal view B ventral view C dorsal disc D skin on the arm E lateral disc F ventral disc G dorsal arm (proximal) H dorsal arm (middle) I ventral arm (proximal) J outer arm spine (proximal) K arm spines (middle) L arm spines (distal). Abbreviations: as adoral shield, ars arm spine, go granular ossicle, gs genital slit, iars inner arm spine, j jaw, oars outer arm spine, rs radial shield, t teeth, tp tentacle pore. Scale bars: 2 mm (A, B); 1 mm (E–G, I); 500 µm (C, H, J, K); 200 µm (D, L).

Arms. Arms at base wide, dorsally flattened, and swollen in first few free segments (Fig. 12G). Arms distalwards from middle part narrowing and more cylindrical (Fig. 10H). Swollen dorsal arm base and proximal end of arm covered with dense granular or polygonal ossicles similar to disc (five or seven grains in 1 mm), on middle segments with slightly separated granular ossicles (six or seven grains in 1 mm), and distally decreasing in size and separated (seven or eight grains in 1 mm) (Fig. 12G, H). Granular ossicles on lateral arm plates slightly separated, but continuing along arm. Ventral arm near arm base covered with granular ossicles similar to ventral disc (seven or eight grains in 1 mm), but less dense on middle half (five or six grains in 1 mm), and distally widely separated or naked (six or seven grains in 1 mm) (Fig. 12I, J). On first few arm segments, tentacle pore with extended tube (Fig. 12J). First tentacle pore without arm spine (Fig. 12F). First arm spine appears at second arm segment, and second arm spine at eighth segment. Inner arm spine cylindrical, one arm segment in length, with blunt thorny tip, and slightly club-shaped (Fig. 12K). Outer arm spine smaller in size, with smooth to thorny tip (Fig. 12K). Both arm spines equal in length at distal end of arm, and compound hook with three or four secondary teeth (Fig. 12L).

Color. In live specimen, reddish brown on dorsal disc and arm, light brown on ventral disc and arm (Fig. 12).

Ossicle morphology

Lateral arm plate with two arm spine articular structures, with large, separated muscle and nerve openings (Fig. 13A). Inner arm spine distalwards from proximal part of arm cylindrical, with terminal projection, and thorny surface (Fig. 13B). Outer arm spine cylindrical, with pointed tip with few thorns (Fig. 13C). Distally arm spine turns into compound hook with four secondary teeth (Fig. 13D). Arm and disc concealed by less dense, wider, and shorter granular ossicles (Fig. 13E). Vertebrae with streptospondylous articulation, dorsally a large longitudinal furrow, ventrally with deep median longitudinal groove with lateral ambulacral canals, no oral bridge, podial basins relatively small (Fig. 13F–J).

Figure 13. 

Asteroschema tubiferum Matsumoto, 1911 (IDSSE-EEB-SW0078) A lateral arm plate B outer arm spine (middle) C inner arm spine (middle) D arm spine (distal) E skin from dorsal arm base (granular ossicles) F–J vertebrae F proximal view G distal view H lateral view I dorsal view J ventral view. Abbreviations: d dorsal, dist distal, lac lateral ambulacral canals, mo muscle opening, no nerve opening, pb podial basin, prox proximal, st secondary teeth, v ventral. Scale bars: 800 µm (F, G, J): 500 µm (B, H, I); 300 µm (A, C–E).

Distribution

325–1800 m depth. New Zealand, Tasman Sea, Kermadec Islands, Bay of Plenty, Hawaii, Sagami Sea, the South China Sea, and Northwest Pacific seamount.

Remarks

Asteroschema tubiferum was first described by Matsumoto (1911), then redescribed by Matsumoto (1917), Baker (1980), and McKnight (2000). These redescriptions are helpful to identify individual morphological character variation. Matsumoto (1911, 1917) mentioned that in Asteroschema tubiferum the first ten arm segments have an extended tube in the tentacle pore (a sheath around the tentacle) that is closely attached to the arm spine and this character is present in our specimens. Previously, A. tubiferum had been recorded from both North and South Pacific Oceans at a wide distribution range. However, this is the first record from the South China Sea. The specimens from our collection concur with previous redescriptions, but we noticed a few variations such as less densely packed ossicles on ventral and lateral arm. The A. tubiferum specimen from the South China Sea collection showed less dense granular ossicles on the ventral disc and arm. Asteroschema tubiferum strongly resembles A. rubrum, A. laeve, and A. inoratum, but the characters of the granulation pattern, tentacle scale on first few arm segments, shape of the arm and inner arm spine characters can be used to distinguish it from these species (Table 2).

Asteroschema salix Lyman, 1879

Figures 14, 15

Asteroschema salix Lyman, 1879: 66–67, fig. 17, figs 466–469; 1882: 277, fig. 22, figs 13–15; Baker 1980: 22; McKnight 2000: 21–22, fig. 6; Olbers et al. 2015: 85, fig. 1A, B; 2019: 51–52, fig. 24–25.

Material examined

China • 1 specimen; South China Sea, Zhongsha Islands, seamount; 15°36.64'N, 116°7.73'E; depth 1775 m; 19 Sep. 2020; Collecting event: stn. SC010; ‘Shenhaiyongshi’ msv leg; preserved in 95% ethanol; GenBank: OK044301, OL712214; IDSSE-EEB-SW0082.

Description

Disc diameter 10 mm, length of arms 145 mm (Fig. 12).

Disc. Disc flat, strongly raised above arms (Fig. 14A, B). Disc covered by thin skin with fine, small, rounded granular ossicles, dense in center (seven or eight grains in 1 mm), but separated at distal edge (six or seven grains in 1 mm) (Fig. 14C, D). Radial shields long, narrow, widely separated distally, convergent proximally, meeting in disc center (Fig. 14C). Genital slits narrow, and vertical on ventral interradii (Fig. 14E). Jaws elongated, covered with granular ossicles but near apex fewer granular ossicles (Fig. 14E). Flattened, spearhead-shaped teeth and granular ossicles that resemble lateral oral papillae at apex of jaw (Fig. 14E). Ventral disc covered with granular ossicles similar to dorsal disc (six or seven grains in 1 mm), slightly separated (Fig. 14E). Adoral shields large, connected to first ventral arm segment, concealed by thin skin with granular ossicles, but plate outline visible (Fig. 14E). Oral shields not discernible and adoral shield spine covered with ossicles (Fig. 14E).

Figure 14. 

Asteroschema salix Lyman, 1879 (IDSSE-EEB-SW0082) A dorsal view B ventral view C dorsal disc D skin on the arm E ventral disc F dorsal arm (proximal) G lateral arm (middle) H ventral arm (proximal) I ventral arm (distal) J outer arm spine (proximal) K arm spines (middle) L arm spines (distal). Abbreviations: as adoral shield, ass adoral shield spine, ars arm spine, go granular ossicle, gs genital slit, iars inner arm spine, j jaw, oars outer arm spine, ots oral tentacle scale, rs radial shield, t teeth, tp tentacle pore. Scale bars: 2 mm (A, B); 1 mm (C, E–H); 500 µm (J, K); 200 µm (D, I, L).

Arms. Arms sub-cylindrical, not swollen, narrower and more cylindrical in distal half of arm (Fig. 14F, G). Dorsal and lateral arm base covered with granular ossicles similar to disc (six or seven grains in 1 mm), on middle segments granular coverage similar to arm base (six or seven grains in 1 mm), and distally decreasing in size and widely separated (grains six or eight in 1 mm) (Fig. 14F, G). On lateral arm plate, granular ossicles continue toward base of arm spine (Fig. 14G, J). Ventral surface of arm base covered with granular ossicles similar to ventral disc but less dense (six or seven grains in 1 mm), widely separated and decreasing in size to naked at middle to distal end of arm (Fig. 14H, I). First tentacle pore without arm spine (Fig. 14E). First arm spine appears at second arm segment, second arm spine at fifteenth or nineteenth segment. Inner arm spine cylindrical, one arm segment in length, flattened, with blunt, thorny tip, slightly club-shaped (Fig. 14J, K). Outer arm spine half as long as inner, with thorny tip (Fig. 14K). Both arm spines equal in length at distal end of arm, and turning into compound hook with 3–5 secondary teeth (Fig. 14L).

Color. In ethanol, pink but when dried, dull brown to whitish (Fig. 14).

Ossicle morphology

Lateral arm plate with two arm spine articular structures, with two large muscle and nerve openings (Fig. 15A). Inner arm spine at proximal and middle half of arm cylindrical, with thorny tip (Fig. 15B). Outer arm spine cylindrical with pointed tip (Fig. 15C). Distally arm spine turns into compound hook with secondary teeth (Fig. 15D). Arm and disc concealed by wider polygonal to rounded granular ossicles (Fig. 15E). Vertebrae with streptospondylous articulation, dorsally a median longitudinal furrow, ventrally with deep median longitudinal groove with lateral ambulacral canals, no oral bridge, podial basins relatively small (Fig. 15F–J).

Figure 15. 

Asteroschema salix Lyman, 1879 (IDSSE-EEB-SW0082) A lateral arm plate B outer arm spine (middle) C inner arm spine (middle) D arm spine (distal) E skin from dorsal arm base (granular ossicles) F–J vertebrae F proximal view G distal view H lateral view I dorsal view J ventral view. Abbreviations: d dorsal, dist distal, lac lateral ambulacral canals, mo muscle opening, no nerve opening, pb podial basin, prox proximal, st secondary teeth, v ventral. Scale bars: 800 µm (B, F–J); 300 µm (A, C, E); 100 µm (D).

Distribution

341–1800 m depth. New Zealand, Tasman Sea, Kermadec Islands, Bay of Plenty, Solomon Island, Coral Sea, Timor Sea, South Africa (off Glenmore), the South China Sea.

Remarks

Asteroschema salix was first described by Lyman (1879), then redescribed by Lyman (1882), Baker (1980), McKnight (2000), and Olbers et al. (2015). These redescriptions are useful to understand individual morphological character variation of A. salix. Specimens from our collection concur with previous redescriptions, but we noticed some variation such as: slightly separated granular ossicles on the disc, fewer granular ossicles on the ventral arm surface, and slightly longer arms. However, most of these morphological variations vary within individual specimens according to previous descriptions (Baker 1980; McKnight 2000). Asteroschema salix strongly resembles A. tubiferum, A. rubrum, A. laeve, A. inoratum, A. arenosum, and A. glaucum but the characters of granulations and ossicle shape on the disc and arm, radial shield, and inner arm spine can be used to delimit A. salix from these species (Table 2). Previously, A. salix had been recorded from the South Pacific Ocean, and South African waters at a wide distribution range. This is the first record from the South China Sea.

Asteroschema cf. lissum H. L. Clark, 1939

Figures 16, 17

Asteroschema lissum H. L. Clark, 1939: 37–39, figs 1–3.

Material examined

China • 2 specimens; South China Sea, Zhongsha Islands, seamount; 13°36.20'N, 113°33.74'E; depth 1515 m; 30 Mar. 2020; Collecting event: stn. SC025; ‘Shenhaiyongshi’ msv leg; preserved in -80 °C; GenBank: OK044300; IDSSE-EEB-SW0079, IDSSE-EEB-SW080. Northwest Pacific • 1 specimen; near Mariana Trench, Southeast of Guam Island, seamount, 12°6.67'N, 141°37.27'E; depth 1160 m; 03 Sep. 2019; Collecting event: stn. SC033; ‘Shenhaiyongshi’ msv leg; preserved in -80 °C; GenBank: OK044299, OL712207; IDSSE-EEB-SW0081.

Description

IDSSE-EEB-SW0079: disc diameter 11.5 mm, length of arms 165 mm, arm base width 3–3.5 mm (Fig. 16).

Disc. Disc flat, slightly raised above arms, swollen in center (Fig. 16A, B). Disc covered with smooth, small granular ossicles (Fig. 16C, D). Granular ossicles dense and small in disc center (six or eight grains in 1 mm), but slightly larger and separated at distal end of radial shield (five or six grains in 1 mm) (Fig. 16C). Radial shields wide, similar in size, curved, slightly raised above disc but not meeting in center (Fig. 16A, C). Radial shields clearly recognizable under thin skin embedded with ossicles (Fig. 16A, C). Genital slits narrow, concealed by polygonal granular ossicles, and vertical on ventral interradii (Fig. 16E). Jaws elongated, mostly naked without granular ossicles (Fig. 16F). Flattened, spearhead-shaped teeth, and six to seven granular ossicles that resemble lateral oral papillae at apex of jaw (Fig. 16F). Ventral disc covered with widely separated small granular ossicles (six or seven grains in 1 mm), but mostly covered by translucent thin skin (Fig. 16F). Adoral shields large, distal edge convex, concealed by thin skin with scattered small, smooth granular ossicles (Fig. 16F). Oral shields not discernible and oral tentacle pore naked without ossicles (Fig. 16F).

Figure 16. 

Asteroschema cf. lissum H. L. Clark, 1939 (IDSSE-EEB-SW0079) A dorsal view B ventral view C dorsal disc D skin on the disc E lateral disc F ventral disc G dorsal arm (middle) H lateral arm (middle) I ventral arm (proximal) J outer arm spine (proximal) K arm spines (middle) L arm spines (distal). Abbreviations: as adoral shield, ass adoral shield spine, ars arm spine, go granular ossicle, gs genital slit, iars inner arm spine, j jaw, oars outer arm spine, ots oral tentacle scale, rs radial shield, t teeth, tp tentacle pore. Scale bars: 2 mm (A, B, G); 1 mm (C, E, F, H, I); 500 µm (J, K); 200 µm (D, L).

Arms. Arms slightly arched, circular, from middle to distal half narrower and more cylindrical (Fig. 16G). Dorsal arm base covered with granular ossicles similar to dorsal disc (six or eight grains in 1 mm), increasingly separated and decreasing in size along the middle segments of the arm (seven or eight grains in 1 mm), distally widely separated (five or six grains in 1 mm) (Fig. 16G, H). On lateral arm plates, granular ossicles widely separated but continuing to base of arm (Fig. 16H, K). Only one or three ventral arm segments near arm base covered with few granular ossicles similar to ventral disc (six or seven grains in 1 mm), then completely naked along the arm (Fig. 16I, J). First arm spine appears at second arm segment, second arm spine at ninth or thirteenth segment (Fig. 16I–K). Inner arm spine initially short, thick with thorny pointed tip, at middle arm cylindrical, taller, one and a half arm segment in length, with flattened thorny tip (Fig. 16J, K). Outer arm spine half as long as inner spine in middle region, with smooth to thorny tip (Fig. 16K). Both arm spines equal in length at distal end of arm, and compound hook with five or six secondary teeth (Fig. 16L).

Color. In live specimen, reddish brown but radial shields slightly lighter in color due to thin skin (Fig. 16).

Ossicle morphology

Lateral arm plate with two arm spine articular structures, with large muscle and nerve openings (Fig. 17A, B). Inner arm spine from proximal and middle half of arm cylindrical, with thorny tip (Fig. 17C). Distally, arm spine turns into compound hook with secondary teeth (Fig. 17D). Arm and disc concealed by less dense, wider, and short granular ossicles (Fig. 17E). Vertebrae with streptospondylous articulation, dorsally a median longitudinal furrow, ventrally with deep median longitudinal groove with lateral ambulacral canals, podial basins relatively small (Fig. 17F–J).

Figure 17. 

Asteroschema cf. lissum H. L. Clark, 1939 (IDSSE-EEB-SW0079) A, B lateral arm plate (external, internal) C outer arm spine (middle) D arm spine (distal) E skin from dorsal arm base (granular ossicles) F–J vertebrae F proximal view G distal view H lateral view I dorsal view J ventral view. Abbreviations: d dorsal, dist distal, lac lateral ambulacral canals, mo muscle opening, no nerve opening, pb podial basin, prox proximal, st secondary teeth, v ventral. Scale bars: 800 µm (F–J); 500 µm (A, C); 300 µm (B, D); 200 µm (E).

Distribution

797–1515 m depth. Maldives, South China Sea, Northwest Pacific.

Remarks

Asteroschema lissum was first described by H. L. Clark (1939), with type locality Maldives waters in the Indian Ocean. This is the first redescription after the original description. The specimens from our collection were close to H. L. Clark’s description but we noticed some differences, such as: characters of radial shields, and granular ossicles at ventral disc and base of arm. We hesitate to fully associate our specimens with Asteroschema lissum or propose a new species, due to uncertainty of these morphological variations. Some of these variations may be affected by size, maturity, and environment (holotype disc diameter 7.5 mm). Asteroschema lissum strongly resembles A. hemigymnum, A. intectum, and A. sublaeve by having similar granular density, and almost naked ventral disc and arms but differs in characters of the radial shields, start of second arm spine, granulation pattern on the disc and arm (Table 2). This is the first record of A. lissum from the South China Sea and the North Pacific Ocean, if it is indeed this species.

Genus Asterostegus Mortensen, 1933

Asterostegus maini McKnight, 2003

Figures 18, 19

Asterostegus maini McKnight, 2003: 386–389, figs 1, 2.

Astroceras elegans McKnight, 1989: 25 (non Astroceras elegans Bell, 1917).

Material examined

China • 2 specimens; South China Sea, near Xisha Islands archipelago, seamount; 16°47.79'N, 113°15.04'E; depth 602 m; 31 Mar. 2020; Collecting event: stn. SC009; ‘Shenhaiyongshi’ msv leg; preserved in -80 °C; GenBank: OK044303; IDSSE-EEB-SW0075; IDSSE-EEB-SW0076.

Description

IDSSE-EEB-SW0076: disc diameter 32.2 mm, length of arms 240–250 mm, height of arm base 9.8 mm (Fig. 18A–M).

Disc. Disc circular and slightly inflated radially, with sunken interradial margins (Fig. 16A). Radial shields elongated, narrow, raised above the disc, extending nearly toward the disc center (Fig. 18B, C). Distal half of radial shield periphery covered by 10–12 (0.44 to 0.75 mm in width) large, club-shaped granules (Fig. 18C). Most of these granules cluster on distal end of radial shield (Fig. 18C). Entire disc, including radial shields, covered by thick skin (Fig. 18A–C). Adoral shields with slightly ovoid outline (Fig. 18E, F). Teeth spearhead-shaped, accompanied by granular domed lateral oral papillae but not visible in wet specimen (Fig. 18D–F). Proximally, adoral shields separated by triangular plate (Fig. 18F) Oral shields absent, and single or double row of two to six rounded, square, or irregular oral interradial plates beyond adoral shields (Fig. 18E, F). One median plate located between distal end of adoral shields, and slightly proximal to rest of oral interradial plates (Fig. 18E, F). Lateral interradial surface of disc slightly vertical and covered by thick naked skin, two conspicuous genital slits inside a large opening (Fig. 18G).

Figure 18. 

Asterostegus maini McKnight, 2003 (A–M IDSSE-EEB-SW0076 N–P IDSSE-EEB-SW0075) A dorsal view B center of dorsal disc C dorsal disc (distal end of radial shields) D ventral disc E, F oral frame G lateral disc H dorsal arm (proximal) I dorsal arm (distal) J lateral arm (proximal) K ventral arm (middle) L, M arm spines (distal) N oral frame O periphery of the disc P oral frame (small transparent ossicles). Abbreviations: as adoral shield, ars arm spine, go granular ossicle, gs genital slit, j jaw, mp median plate, oip oral interradial plate, rs radial shield, t teeth, tp tentacle pore, ts tentacle scale. Scale bars: 6 mm (A); 2 mm (B–H, N); 1 mm (I–K, O, P); 500 µm (L); 200 µm (M).

Arms. Arms simple, strong, and not branching (Fig. 18A). Cross section of arm base slightly rectangular (7.2 mm in width and 8.5 mm high), but distal half of arm more cylindrical and narrower (Fig. 18H–J). Proximal to middle region of dorsal arm slightly flattened, ventral surface slightly arched, and lateral surface vertical (Fig. 18J, K). Whole arm concealed by thick skin (Fig. 18H–K). Lateral arm plate on proximal half of arms bears three to five club-shaped granules (0.4–0.6 mm in width) (Fig. 18H), decreasing in size and number along arm and vanishing on distal half of arm (Fig. 18I). Ventral arm covered by naked skin (Fig. 18K). First one to two tentacle pores lack arm spines; second or third pore with two or three arm spines, similar in size (Fig. 18D). At proximal end of arms, arm spines short, thick, ovoid with more or less rounded tip with rough surface, at middle of arms club-shaped, transversely flattened (Fig. 18J, K). Arm spines turn into hook with two to three secondary teeth at distal end of arms (Fig. 18L, M).

Color. In live specimen, dorsal disc interradially dark brown but radial shields light brown. Ventral and lateral disc whitish brown, dorsal surface of proximal and middle regions of arms bright brown/red. Lateral and ventral surface of arms whitish brown, granules creamy white (Fig. 18).

Ossicle morphology

Lateral arm plate with two arm spine articular structures, middle half of arm with large and wide, separated muscle and nerve openings, depression on inner side (Fig. 19A, B). Arm spines large, short, flattened, and club-shaped with thorny surface (Fig. 19C, D). Vertebrae with streptospondylous articulation. Vertebrae on proximal to middle half of the arm large, flat with deep ventral groove, no oral bridge (Fig. 19E, F). Vertebrae on distal half of arm slightly longer, dorsally large median longitudinal furrow, and deep median longitudinal groove on ventral side, with oral bridge (Fig. 19G, H).

Figure 19. 

Asterostegus maini McKnight, 2003 (IDSSE-EEB-SW0076) A, B lateral arm plate (external, internal) C, D arm spine (middle) E, F vertebrae (middle) E proximal view F distal view G–K vertebrae (distal) G proximal view H distal view I lateral view J dorsal view K ventral view. Abbreviations: asa arm spine articular structure, d dorsal, de depression, dist distal, mo muscle opening, ob oral bridge, prox proximal, v ventral. Scale bars: 800 µm (E, F); 500 µm (A, C, D, I, K); 300 µm (B, G, H, J).

Distribution

417–602 m in depth. New Zealand (Cook Island), South China Sea.

Remarks

Asterostegus maini was first described by McKnight (2003), with type locality Cook Islands, South Pacific Ocean, and Okanishi and Fujita (2014) redescribed it. However, this is the first record of Asterostegus maini since the holotype. Here we recorded two specimens from the South China Sea (disc diameter 26 mm and 32.2 mm) and both are larger than the holotype (disc diameter 22 mm). Currently, three species belong to the genus Asterostegus: A. maini McKnight, 2003, A. tuberculatus Mortensen, 1933, and A. sabineae Okanishi & Fujita, 2014. Asterostegus tuberculatus differs from A. maini in granules being scattered across the whole disc including the radial shield, and only two to three stump-like granules on the dorsolateral arm plate (McKnight 2003; Okanishi and Fujita 2014). Asterostegus sabineae differs from A. maini in large stump-like granules on the radial shield, only one oral interradial plate, and one or two large stump-like granules on the dorsolateral arm plate (Okanishi and Fujita 2014). The number of oral interradial plates and their arrangement are highly variable within and between individuals (Fig. 18E, F, N). Previous studies on the holotype showed only one row with two to five interradial plates (McKnight 2003; Okanishi and Fujita 2014). However, in our specimens, these are arranged in one or two rows with two to seven interradial plates in total (Fig. 18E, F, N). Therefore, one of the key morphological characters in the genus Asterostegus, the arrangement of oral interradial plates has to be modified. One specimen from the present study (IDSSE-EEB-SW0075, 26 mm disc diameter) showed some morphological variations, such as: small granules on the periphery of the disc and on few small areas on the ventral disc (Fig. 18O, P). These granules are extremely small compared to those on the radial shields and arms. In the specimens from the present study, first arm spines started from second or third arm segment, but in the holotype, it started from the fourth arm segment. However, except for these small morphological variations, both specimens were similar to the holotype description.

Family Gorgonocephalidae Ljungman, 1867

Subfamily Gorgonocephalinae Döderlein, 1911

Genus Astrodendrum Döderlein, 1911

Astrodendrum cf. sagaminum (Döderlein, 1902)

Figures 20, 21

Astrodendrum sagaminum Döderlein, 1902: 321–322; 1911: 38–39, figs 2, 3–5, 7, 8; 1927: 32, 92; H.L. Clark 1911: 292–293; A. H. Clark 1916a: 185; Liao 2004: 109–111, fig. 52.

Material examined

China • 1 specimen; South China Sea, East side from Zhongsha Islands, seamount; 16°22.11'N, 116°06.60'E; depth 1619 m; 09 Aug. 2020; Collecting event: stn. SC028; ‘Shenhaiyongshi’ msv leg; preserved in -80 °C; GenBank: OK044304; IDSSE-EEB-SW0104.

Description

Disc diameter 62 mm (Fig. 20A, B).

Disc. Dorsal disc slightly inflated, swollen in the center (Fig. 20A, B). Radial shields elongated, tapered at proximal end, extending to center of disc (Fig. 20C, D). Entire disc covered by skin with conical ossicles of various size (0.4–0.7 mm high) and widely separated and scattered (Fig. 20C–E). Genital slits conspicuous, interradial margin covered by two rows of higher than wide conical ossicles (Fig. 20F, G). Ventral disc almost naked, but micro-granular ossicles visible on oral region (Fig. 18H). Oral area covered by smooth skin with few scattered small granular ossicles, exposing adoral and oral shield outlines (Fig. 20H). Oral plates flat, polygonal, and slightly in contact with adoral shields (Fig. 20H). Adoral shields short, square. Oral papillae and teeth spiniform (Fig. 20H), several vertical rows of teeth on dental plate (possibly tooth papillae at ventral edge).

Figure 20. 

Astrodendrum cf. sagaminum (Döderlein, 1902) (IDSSE-EEB-SW0104) A dorsal view B ventral view C dorsal disc (center) D dorsal disc (distal edge) E radial shield F, G ventral disc H oral frame I, J ventral view of arm base K ventral view of after second arm branch L, M dorsal view of second arm branch N lateral view of arm (middle) O lateral view of arm (pedicellariae with baseplate) P, Q variations of arm spine on distal end of the arm. Abbreviations: ars arm spine, co conical ossicles gs genital slit, os oral shield, peb = pedicellarial band, rs radial shield, t teeth, tp tentacle pore. Scale bars: 16 mm (A, B); 2 mm (C, D, F, L); 1 mm (E, H–K, M, N); 500 µm (G, O); 200 µm (P, Q).

Arms. Arms branched at least eight to nine times, flexible dorso-ventrally, flat ventrally, arched dorsally (Fig. 20I–N). Ventral arm surface covered by smooth skin; proximal half with widely scattered small, flat, polygonal granular ossicles (Fig. 20I–K). Dorsal arm surface covered by polygonal or domed plates and between these pedicellarial bands (Fig. 20L–O) that appear after second arm fork, covering whole lateral to dorsal area of arm, creating annulated appearance (Fig. 20L). First arm segment lacks spines, next four to six with two arm spines, thereafter two or three arm spines per segment (Fig. 20I–K). Ventral arm spines similar in size, smaller, unevenly pointed, distally turning into hooks with 2–3 secondary teeth (Fig. 20K, P, Q).

Color. In live specimen, whole specimen brown, but radial shields, oral regions, and ventral arms lighter than disc (Fig. 20).

Ossicle morphology

On middle half of arm, lateral arm plates with perforations on ventral side, large muscle opening and small nerve opening (Fig. 21A). Pedicellarial bands formed by approximately 12 articulating tubercles at curved distal end of baseplate and these articulations have a single foramen per tubercle for pedicellariae with one secondary tooth (Fig. 21B, C). Ventral arm spines on distal end of arm transformed into hook with two or three secondary teeth (Fig. 21D). Pedicellariae differ from ventral arm spine by having smooth apophysis (Fig. 21C, D). Vertebrae with streptospondylous articulation with smooth lateral furrows and paired openings in lateral side of vertebrae for lateral ambulacral canals, no oral bridge (Fig. 21F–N).

Figure 21. 

Astrodendrum cf. sagaminum (Döderlein, 1902) (IDSSE-EEB-SW0104) A lateral arm plate (middle) B plan view of baseplate C pedicellariae D arm spine on distal end E–J vertebrae on middle half of arm E proximal view of branch vertebrae F proximal view G distal view H lateral view I dorsal view J ventral view K–N vertebrae on distal end of arm K distal view L proximal view M lateral view N ventral view. Abbreviations: ap articular pad of the base, au auricle, d dorsal, dist distal, fo foramina of the base, fs fossa between adjacent tubercles, hd head of the apophysis, lac lateral ambulacral canals, mo muscle opening, no nerve opening, pd pedicel of the apophysis, prox proximal, pt primary tooth of the blade, sh sheath of the baseplate, su sulcus of tubercle head, v ventral. Scale bars: 800 µm (E–J); 500 µm (A, B); 300 µm (K–N); 200 µm (D); 80 µm (C).

Distribution

90–1300 m depth. South China Sea, Japan, East China Sea, Sri Lanka.

Remarks

The specimen is similar to the holotype description by Döderlein (1902), and the redescriptions of Döderlein (1911, 1927), Liao (2004) and Okanishi and Fujita (2018), but showed some morphological variations especially on the disc (Fig. 20). Therefore, we hesitate to fully associate our specimen with Astrodendrum sagaminum. All descriptions mentioned granules on both dorsal and ventral disc, but in the present specimen, the dorsal disc is covered with widely separated conical stump-like granules and the ventral disc is covered with widely scattered micro-polygonal ossicle plates in naked skin. However, Baker (1980) mentioned that the granular pattern on the disc was not a suitable morphological feature to delimit species in the genus Gorgonocephalus. This is the first record of Astrodendrum sagaminum from the South China Sea, if this is indeed that species.

Discussion

The molecular phylogenetic trees of these species of Gorgonocephalidae and Euryalidae were in agreement with previous studies (Okanishi and Fujita 2013; Christodoulou et al. 2019; O’Hara et al. 2019). Previous molecular studies indicated that intraspecific genetic distance ranges approximately from 0.5% to 6.4%, with a mean of 2.2%, but species from the family Euryalidae usually showed less than 2% mean genetic distance (Okanishi et al. 2011, 2018; Okanishi and Fujita 2013; Boissin et al. 2017). In this study, we focused on the genera Asteroschema, Asterostegus, and Astrodendrum. In addition, we included species from the genus Ophiocreas due to their similar morphology to Asteroschema species. The species in the genus Asteroschema were difficult to analyze only morphologically due to great similarity in most morphological characters. Previous studies divided Asteroschema into three groups according to ossicle shape on the disc and arms, but still many species within these groups are hard to identify. In this study, we successfully managed to obtain the COI and 16S sequences from only one of the proposed new species, Asteroschema shenhaiyongshii sp. nov., which suggests a close relationship to A. bidwillae according to genetic distance (2.56%), but A. shenhaiyongshii sp. nov. is pentamerous and shows no signs of fission. It also has dense granular ossicle coverage on the ventral disc and ventral arm surface (Suppl. material 2: Table S2; Fig. 6). Therefore, we consider these two as sibling species. According to the present study, we suggest a species complex within Asteroschema tubiferum due to morphological variations between specimens from the South China Sea and New Zealand. However, we found significantly low genetic distances between the specimens identified as A. cf. lissum, A. tubiferum, A. rubrum, and A. salix (2.79±0.66% SE) (Suppl. material 2: Table S2). Therefore, understanding key morphological differences and intraspecific genetic distance range are important to delimit Asteroschema species. Asteroschema cf. lissum was recognized here as intermediate species between A. salix and A. tubiferum due to genetic distance values between these species. In the ML tree of the family Euryalidae, all Ophiocreas species cluster with Asteroschema oligactes, A. migrator, A. edmondsoni, A. ajax, and A. horridum. All these Asteroschema species have conical ossicles or annular bands on the arms. These two clades may correspond to one of these genera each or one of them may belong to both genera (making them synonymous) and the other to a putative new genus, but since the type species of both genera have not been sequenced yet, it is impossible to decide. Thus, the present study concurs with previous molecular studies in the hypothesis that Asteroschema may be polyphyletic, but may instead be paraphyletic with the genus Ophiocreas, and the present morphological differentiation between these two genera can be questioned (Okanishi and Fujita 2013; Christodoulou et al. 2019; O’Hara et al. 2019). A comprehensive morphological and molecular taxonomic revision, including examination of type specimens of all Asteroschema and Ophiocreas species is needed to understand the key morphological characters and genetic differences. We tentatively place our new species in Asteroschema, but acknowledge that they may later be found to belong in Ophiocreas.

The genus Asterostegus includes only three species, and is closely related to Astroceras, but a previous phylogenetic analysis recognized it as monophyletic and belonging in the family Euryalidae (Okanishi and Fujita 2013). The interspecific genetic distance (3.09±0.75% SE) within the genus Asterostegus was low and similar to other Euryalidae species (Suppl. material 2: Table S2). The genetic distance of the genus Gorgonocephalus was significantly lower than in Asteroschema. The molecular phylogenetic analysis of Astrodendrum and Gorgonocephalus showed two main clades, and previous studies showed that Gorgonocephalus may be polyphyletic (Okanishi and Fujita 2013; Christodoulou et al. 2019; O’Hara et al. 2019). In the present study, Astrodendrum cf. sagaminum clustered with Gorgonocephalus sundanus and G. pustulatum. The type species Gorgonocephalus caputmedusae (Linnaeus, 1758) clustered with G. chilensis, G. eucnemis, G. arcticus, and G. tuberosus (Fig. 3, Suppl. material 3: Table S3). We suggest that A. sagaminum should belong in the genus Gorgonocephalus, or alternatively, Gorgonocephalus could be split into two genera, but a more thorough study with more genes and more specimens should be performed, before this step is taken.

Most of the species from the present study were collected from deep water in the South China Sea. Previous studies from the South China Sea recorded only few Asteroschema species, but found no representatives of the genera Astrodendrum and Asterostegus. According to the present study, the ophiuroid diversity of the South China Sea may be higher than previously known and future expeditions to the South China Sea deep-sea seamounts may discover even more species. The present study suggests a wider distribution of Euryalida species from the South-Pacific to the North-Pacific regions than previously expected.

Acknowledgements

The authors want to thank the crews of the vessel ‘Tansuo 1’ and the pilots of the HOV ‘Shenhaiyongshi’. The authors also thank the members of the marine ecology and evolutionary biology laboratory at the Institute of Deep-sea Science and Engineering, CAS for their help in sample collection and analysis. Many thanks to Hou Xue and Zhi Zheng for their help in acquiring SEM images of specimens. Many thanks also to the reviewers and the editor, who helped to improve the manuscript. This work was supported by the Major Scientific and Technological Projects of Hainan Province (ZDKJ2019011), the National Key Research and Development Program of China (2016YFC0304905, 2018YFC0309804), and Strategic Priority Research Program of the Chinese Academy of Sciences (XDA22040502).

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Supplementary materials

Supplementary material 1 

Table S1

Hasitha Nethupul, Sabine Stöhr, Haibin Zhang

Data type: Primers (text)

Explanation note: COI and 16S primers and PCR cycles used in this study (Hoareau and Boissin 2010; Okanishi and Fujita 2013).

This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.
Download file (34.75 kb)
Supplementary material 2 

Table S2

Hasitha Nethupul, Sabine Stöhr, Haibin Zhang

Data type: Genetic distance % (COI)

Explanation note: Genera Asteroschema, Ophiocreas and Asterostegus, pairwise distance values based on 592 bp mitochondrial COI sequences, calculated using the Kimura 2-parameter method with 1,000 bootstrap replicates (Value in blue color represent Standard Error). Abbreviations: AUS Australia, CAN Canada, CS Caribbean Sea, JAP Japan, MAD Madagascar, NCA New Caledonia, NWP Northwest Pacific, NZ New Zealand, PAO Pacific Ocean, SCS South China Sea.

This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.
Download file (26.68 kb)
Supplementary material 3 

Table S3

Hasitha Nethupul, Sabine Stöhr, Haibin Zhang

Data type: Genetic distance % (COI)

Explanation note: Genera Gorgonocephalus and Astrodendrum, pairwise distance values based on 730 bp mitochondrial COI sequences, calculated using the Kimura 2-parameter method with 1,000 bootstrap replicates (Value in blue color represent Standard Error). Abbreviations: AN Antarctic Sea, AUS Australia, CAN Canada, JAP Japan, NZ New Zealand, SCS South China Sea, SWE Sweden.

This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.
Download file (18.96 kb)
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