Research Article
Print
Research Article
A new species of Neohagenulus Traver, 1938 from Hispaniola (Ephemeroptera, Leptophlebiidae, Hagenulinae, Hagenulini)
expand article infoMichel Sartori§
‡ Museum of Zoology, Lausanne, Switzerland
§ University of Lausanne, Lausanne, Switzerland
Open Access

Abstract

Here, I report a new species of the genus Neohagenulus Traver, 1938 from the Dominican Republic. The genus was believed to be endemic to Puerto Rico until now. Neohagenulus hodeceki sp. nov. is described at the nymphal stage. Some discussion on the tribe Hagenulini is also provided.

Keywords

Borinquena, Careospina, Hagenulus, morphology, new species, nymph, sexual dimorphism

Introduction

Nine genera of the family Leptophlebiidae have been found on four islands of the Greater Antilles, Cuba, Hispaniola, Jamaica and Puerto Rico (Naranjo Lopez and Peters 2016) and some of them are sometimes considered to be subgenera (Kluge 1994). Except for Farrodes Peters, 1971, Hagenulopsis Ulmer, 1920 and Hagenulus Eaton, 1882, which include species distributed in the continental Americas, all other genera are endemic to the Greater Antilles. These are Borinquena Traver, 1938 with two species in Puerto Rico and one in Cuba, Careospina Peters, 1971 with four species in Cuba and one in Hispaniola, Neohagenulus Traver, 1938 with three species in Puerto Rico and Poecilophlebia Kluge, 1994, Traverina Peters, 1971 and Turquinophlebia Kluge, 1994, each with one, two and one species endemic to Cuba, respectively (Naranjo Lopez et al. 2019).

Hispaniola is one of the less-studied for mayflies of the four islands. Two leptophlebiid species are currently known, Hagenulus eatoni Banks, 1924 and Careospina annulata Peters, 1971. The former is only known by the type series, which consists of 11 pinned females collected in 1912 at Diquini, Haiti and the latter is known only by the holotype, a pinned male imago collected in 1934 from Mont La Hotte, Haiti. Kluge (1994) considered the systematic position of C. annulata as unclear, pending the description of the nymph to fix its status.

Here, I report the presence of the genus Neohagenulus on Hispaniola, from the Dominican Republic, based on nymphs which are described as a new species. This is the first species of this genus known outside of Puerto Rico.

Material and methods

The specimens have been collected in the Dominican Republic during a field trip in summer 2021.

Nymphs were preserved in 100% ethanol. Nymphal habitus were photographed using a Canon EOS 6D camera and the Visionary Digital Passport imaging system (formerly available and distributed by Dun Inc., Virginia) and processed with Adobe Photoshop Lightroom and Helicon Focus version 5.3.

Two nymphs were dissected in Cellosolve (2-Ethoxyethanol) with subsequent embedding in Euparal medium and mounting on slides. Fore- and hind wingpads of a submature female nymph were dissected and subimaginal wings examined. Microscopic pictures were taken using an Olympus BX51 microscope coupled with an Olympus SC50 camera; photographs were enhanced with Olympus Stream Basic 2.3.2 stacking software and Adobe Photoshop version 21.2.2.

The material is deposited in the collections of the Museum of zoology, Lausanne (MZL) and the Museo National de Historia Natural "Prof. Eugenio de Jesús Marcano", Santo Domingo, Dominican Republic (MNHNSD).

Results

Neohagenulus hodeceki sp. nov.

Material

Holotype. Dominican Republic male nymph in ethanol, La Vega Province, Valle Nuevo National Park, 18°52'01"N, 70°34'44"W, 12 Jul. 2021, ca 900 m a.s.l., J. Hodeček leg. (GBIFCH00834690) [MZL] Paratypes. 5 nymphs in ethanol [MNHNSD] (MNHNSD 11.05 – MNHNSD 11.09), 12 nymphs in ethanol (GBIFCH00834691), 2 female nymphs on slide (GBIFCH00604114-GBIFCH00604115), same data as holotype. [MZL]

Other material

Dominican Republic 1 nymph, La Vega Province, Armando Bermúdez National Park, 19°04'02"N 70°51'50.7"W, ca 1100 m a.s.l., 15 Jul. 2021, J. Hodeček leg. (GBIFCH00834694) [MZL]

Etymology

The new species is named after its collector, Dr Jiří Hodeček (CHUV, Lausanne), forensic entomologist.

Description

Nymph (not mature): body length up to 7 mm, cerci slightly longer than body length, paracercus longer than cerci.

Coloration. Cuticular coloration evenly light brown on whole body; hypodermal coloration as in Fig. 1: head washed with grey, darker between ocelli, convex band between antennae, upper portion of male eyes black; prothorax greyish laterally and on posterior margin, mesothorax with blackish tracheation laterally, with maculae posteriorly; legs light brown, apex of femora with blackish dots, tarsi darker than tibiae; abdominal tergites I–V light brown with posterior black band larger laterally and with two antero-submedian maculae, tergites VI–VIII greyish brown, posterior margin and sagittal line light brown, tergite IX paler than previous ones, tergite X medium brown; sternites uniformly light brown, last two darker; cerci uniformly light brown.

Figure 1. 

Neohagenulus hodeceki sp. nov., nymphal habitus A dorsal view B lateral view C ventral view. Scale bar: 1 mm.

Mouthparts. Labrum (Fig. 2A) larger than clypeus, about two times broader than long, dorsally with two rows of long and thin setae, proximal row very close to distal margin, tuft of long setae laterally, more abundant at anterolateral corner, ventrally with two submedian fields of long and stout setae, antero-median emargination smooth, with four equally sized denticles (Fig. 2B). Mandibles outer margin regularly convex, with tuft of small and thin setae in middle, with outer and inner incisors composed of three teeth, outer margins slightly serrated, prostheca with stout and long process and well-developed tuft of thin setae (Fig. 2C). Maxillary palp three-segmented (Fig. 2D), second segment ca 1.25× length of segment 1, segment 3 conical, outer margin concave near apex, ca 1.5× longer than wide and 0.50‒0.60× length of segment two, crown of the galea-lacinia with subapical setae arranged in two rows of 8‒9 laterally and 11‒12 centrally. Hypopharynx with lingua convex, with deep incision distally, lateral processes well developed, but shorter than lingua, slightly curved inwards; superlinguae laterally expanded, distal margin covered with long setae up to the tip (Fig. 3B). Labium (Fig. 3A) with narrow glossae, with stout setae at apex, paraglossae rhomboid, outer margin almost straight, with numerous long setae laterally and apically; labial palp three-segmented, first and second segments subequal in length, or segment two slightly longer, third segment ca 0.35‒0.40× length of segment two, 2.5‒2.6× longer than wide.

Figure 2. 

Neohagenulus hodeceki sp. nov. mouthparts A labrum in dorsal view B antero-median emargination of the labrum C left mandible D maxilla. Scale bars: 0.2 mm (A, C, D); 0.05 mm (B).

Thorax. Forelegs with femora ca 2.5× longer than wide, outer margin with row of very long and thin setae, together with apical and subapical rows of long, stout and pointed setae, inner margin with subapical row of long and stout setae (shorter than those on the outer margin), with small stout setae in proximal part, dorsal surface with scattered small to medium-sized stout setae; tibiae shorter than femora, with long and thin setae on outer margin, inner margin with several rows of stout small to medium-sized setae; tarsi only with thin setae, claw moderately hooked, with a row of 12‒14 teeth, increasing in size distally (Fig. 3C). Midlegs similar to forelegs, except inner margin of tibiae with fewer stout setae. Hindlegs with femora almost 3× longer than wide, similar to forelegs in ornamentation except outer margin with shorter and less numerous thin setae; hind tibia shorter than femora, with outer margin covered with long and thin setae, together with short and medium-sized stout and pointed setae, inner margin with marginal row of short and submarginal row of longer stout setae (Fig. 3D). Fore wingpad markedly different between male and female nymphs: in males, evenly brown with veins hardly visible (Fig. 1A), in females with longitudinal veins well marked, crossveins flanked with dark brown bands (Fig. 4A); in both sexes, base of wingpads tinted with brown in costal, subcostal and anal fields. Hind wingpad very small, tinted with greyish brown at base, with costal process large and slightly pointed, almost as long as the rounded apex of wing (Fig. 4B).

Figure 3. 

Neohagenulus hodeceki sp. nov. mouthparts and legs A labium B hypopharynx C claw D detail of hind tibia. Scale bars: 0.2 mm (A–C); 0.1 mm (D)

Abdomen . Posterior margin of tergites I‒III smooth, of segments IV‒VIII with small needle-like denticles, slightly increasing in size posteriorly, tergites IX and X with triangular denticles; posterior margin of sternite IX concave in the middle (Fig. 4C); posterolateral projections on abdominal segments II‒IX, increasing in size posteriorly (Fig. 4C); gills present on segments I‒VII alike, each gill deeply forked almost at base (Fig. 4D) with purplish longitudinal and lateral tracheations, size of gills in decreasing order: II=V>III>IV=VI>I>VII. Cerci with whorls of small setae at the end of each segment.

Figure 4. 

Neohagenulus hodeceki sp. nov. thorax and abdomen. A female fore wingpad B female hind wingpad (same specimen) C abdomen in ventral view (arrows indicate posterolateral projections on segments II‒IX) D gill IV. Scale bars: 0.5 mm (A, D), 0.2 mm (B), 1 mm (C).

Male imago, female imago, eggs unknown.

Discussion

The new species is attributed to the genus Neohagenulus mainly based on the posterolateral projections present on abdominal segments II‒IX. In the redescription proposed by Peters (1971), these projections are present on segments III‒IX for Puerto Rican species; this is the main character to separate nymphs of Neohagenulus from those of Careospina, where these projections are located on segments (V) VI‒IX. Another closely related (sub)genus is Borinquena, as defined by Kluge (1994), which possesses an elongated third segment of labial palp, longer than half the length of second segment, which is not the case in N. hodeceki (about one-third the length of second segment).

Another consideration for placement of the new species in the genus Neohagenulus is the size of the costal projection on the hind wingpad, suggesting that the hind wing of this species possesses a well-developed and long process. In Careospina, the costal process is present and normally developed (Peters 1971: fig 29), whereas it is hypertrophied in Neohagenulus (Peters 1971: figs 34‒42), reaching the apex of the wing. This character can also be found in Borinquena (Kluge 1994: fig 53). All in all, morphological differences between nymphs of Borinquena, Careospina and Neohagenulus are tiny; in N. hodeceki, the length of the second segment of the maxillary palp is about 1.25× the length of the first segment, a character which matches the diagnosis of Careospina rather than that of Neohagenulus, where both segments are of equal size. These three genera seem easier to separate at the male imaginal stage, based on the shape of the hind wing, forceps and penes (Peters 1971; Kluge 1994).

Despite of this, N. hodeceki can be separated from species of Careospina (all known from Cuba) and Borinquena (Puerto Rico and Cuba) at the nymphal stage by the number of posterolateral projections on the abdomen and from species of Neohagenulus (all known from Puerto Rico), by the size of the second segment of the maxillary palp.

A remarkable character is the difference in the fore wingpad coloration between male and female nymphs; this dimorphism is not reported within Neohagenulus or Careospina species, although females often have costal and subcostal fields of forewing more tinted than in males. This difference is nevertheless testified for the species Hagenulus jamaicensis Peters, 1971 where male forewing is hyaline except some crossveins “surrounded with brown clouds” at the tip of the wing, whereas the female forewing exhibits almost all crossveins “surrounded with dark brown clouds” (Peters 1971: figs 58, 61).

Among the tribe Hagenulini (sensu Monjardim et al. 2020) and according to Kluge (1994), Hagenulus sensu lato, or Hagenulus/fg2, is composed of the following taxa: Borinquena, Careospina, Hagenulopsis, Hagenulus s.s., Neohagenulus, Poecilophlebia and Turquinophlebia, which form a monophyletic lineage. The genus Borinquena is reported from Hispaniola only by fossil records from Dominican amber, together with the extinct genus Hagenulites Staniczek, 2003 (Staniczek 2003; Staniczek et al. 2017).

The fact that the genus Borinquena is known from Puerto Rico and Cuba by five extant species and from Hispaniola by four extinct species indicates a larger distribution of the genus in the Miocene. Due to the lack of prospection on Hispaniola, it is therefore possible that the genus is still present but overlooked. In any case, a better understanding of the diversity of the tribe Hagenulini on Hispaniola is required before any biogeographical or phylogeographic attempt.

Neohagenulus hodeceki is the third extant leptophlebiid species reported from Hispaniola, the first one for almost a century and the first one known at the nymphal stage. Although I fully agree that the knowledge of the imaginal stages is of prime importance, I think it is noteworthy to formally mention and describe this taxon due to the scarcity of data on the leptophlebiids of Hispaniola. Considering the diversity reported for fishes (Rodriguez-Silva and Schlupp 2021) and caddisflies (Flint and Sikora 2004) for instance, we might deduct that mayfly diversity in Hispaniola is greatly underestimated.

Acknowledgements

My sincere thanks to Jiří Hodeček (CHUV, Lausanne) and Jiří Pirkl for collecting these interesting specimens. Dr Hodeček’s fieldwork was made possible by a collecting permit and an export permit (VAPB-08862) issued by the Ministry of Environment and Natural resources, Santo Domingo, which is greatly acknowledged. Gabriel de los Santos (MNHNSD) is thanked for his logistic support. I appreciated the careful review and suggestions made by Arnold Staniczek (SMNS, Stuttgart) and Luke M. Jacobus (IUPC, Columbus).

References

  • Flint Jr OS, Sykora JL (2004) Caddisflies of Hispaniola, with special reference to the Dominican Republic (Insecta: Trichoptera). Annals of Carnegie Museum 73(1): 1‒60.
  • Kluge NJ (1994) A revision of Leptophlebiidae of Cuba (Ephemeroptera). Zoosystematica Rossica 2[1993]: 247‒285.
  • Monjardim M, Paresque R, Salles FF (2020) Phylogeny and classification of Leptophlebiidae (Ephemeroptera) with an emphasis on Neotropical fauna. Systematic Entomology 45: 415‒429. https://doi.org/10.1111/syen.12402
  • Naranjo López C, Peters JG (2016) New records of Ephemeroptera (Insecta) from Jamaica and the Dominican Republic. In: Yoshimura M, Takemon Y (Eds) International Progress in Ephemeroptera and Plecoptera Research (13th International Conference on Ephemeroptera and 17th International Symposium on Plecoptera). Biology of Inland Waters Supplement 3: 83‒91.
  • Naranjo López C, Peters JG, López del Castillo P (2019) Ephemeroptera (Insecta) in Cuba. Insecta Mundi 736: 1‒52.
  • Rodriguez-Silva R, Schlupp I (2021) Biogeography of the West Indies: a complex scenario for species radiations in terrestrial and aquatic habitats. Ecology and Evolution 11: 2416‒2430. https://doi.org/10.1002/ece3.7236
  • Staniczek AH (2003) New fossil mayflies from Dominican amber (Insecta: Ephemeroptera: Leptophlebiidae: Atalophlebiinae). Stuttgarter Beiträge zur Naturkunde, Serie B (Geologie und Paläontologie) 341: 1‒22.
  • Staniczek AH, Godunko RJ, Krzeminski W (2017) A new fossil mayfly species of the genus Borinquena Traver, 1938 (Ephemeroptera: Leptophlebiidae: Atalophlebiinae) from Miocene Dominican Amber. Annales Zoologici 67: 113‒119. https://doi.org/10.3161/00034541ANZ2017.67.1.013