Research Article |
Corresponding author: Hyojoong Kim ( hkim@kunsan.ac.kr ) Academic editor: Xue-xin Chen
© 2021 Ju-Hyeong Sohn, Cornelis van Achterberg, Yunjong Han, Hyojoong Kim.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Sohn J-H, van Achterberg C, Han Y, Kim H (2021) A new species of the genus Hylcalosia Fischer (Hymenoptera: Braconidae: Alysiinae) from South Korea, with a key to the Korean species. ZooKeys 1070: 31-40. https://doi.org/10.3897/zookeys.1070.73377
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The species of the genus Hylcalosia Fischer, 1967 (Braconidae: Alysiinae) from South Korea are revised. One species, Hylcalosia bicolor sp. nov., is new to science. They are described and illustrated herein and an identification key to the Korean species is added. In addition, the DNA barcode region of the mitochondrial cytochrome c oxidase subunit I (COI) has been analysed for the new species and H. sutchanica is used for genetic comparison.
COI barcode, cyclostome, koinobiont, natural enemy, parasitoid wasp, systematics, taxonomy
The subfamily Alysiinae, which is one of the large taxa in the family Braconidae, occurs worldwide and contains over 2,440 valid species (
Hylcalosia Fischer, 1967 is a small genus of Alysiinae, which includes 18 species (
In this study, we present new morphological characters and the barcoding sequences of the COI region of H. bicolor sp. nov. and one previously-recorded species, H. sutchanica. Descriptions, diagnoses, an identification key and photographs of the diagnostic characters are provided.
Samples used in this study were collected with Malaise traps in South Korea at the DMZ Botanical Garden, Mandae-ri, Haean-myeon, Yanggu-gun, Gangwon-do. Sorting and preparation were done at the Animal Systematics Lab. (ASL), Department of Biology, Kunsan National University (
A LEICA DMC2900 digital camera and a LEICA M205C stereomicroscope (Leica Geosystems AG) were used for photography and several pictures were taken for each height using multi-focusing technology. LAS V4.11 (Leica Geosystems AG, Wetzlar, Germany) and HeliconFocus 7 (Helicon Soft, Kharkiv, Ukraine) software were used for stacking work. After stacking work, illustrations were created using Adobe Photoshop CS6.
Extraction of DNA was done in ASL,
Sequence alignments were performed in MEGA version 7 (
A total of 563 bp of the COI barcode region were sequenced from H. bicolor sp. nov. and H. sutchanica which were deposited in GenBank (accession numbers MZ717196, MZ717194). Pairwise distances were estimated by using the P-distance model with the option for pairwise deletion. As a result, H. bicolor sp. nov. showed a fairly large genetic difference of 6% from H. sutchanica.
Holcalysia Cameron, 1910: 6 [nec Cameron, 1905]; Shenefelt, 1974: 993. Type species: Holcalysia ruficeps Cameron, 1910.
Hylcalosia Fischer, 1967: 125; Shenefelt, 1974: 993; Chen & Wu, 1994: 85; Belokobylskij, 1998: 297; Zheng, Chen & Yang, 2012: 454; Belokobylskij, 2015: 530. Type species: Holcalysia ruficeps Cameron, 1910.
First flagellomere distinctly shorter than second (Figs
Unknown.
Palaearctic (East) and Oriental Regions.
1. | Second metasomal tergite 1.4–1.5 times longer than third tergite (Fig. |
H. sutchanica Belokobylskij, 1992 |
– | Second metasomal tergite 1.1–1.2 times as long as third tergite (Fig. |
2 |
2. | Head entirely black; vein r of fore wing comparatively short (Fig. |
H. bicolor sp. nov. |
– | Head (except stemmaticum) brownish-yellow; vein r of fore wing medium-sized, 0.9 times as long as maximum width of pterostigma; metasoma black; first tergite gradually widened posteriorly; eye in dorsal view about as long as temple | H. maetoi van Achterberg, 1983 |
Holotype
. ♀ (
This species is similar to H. verticalis Zhu, van Achterberg & Chen, 2018 from China because of the vertical vein r-m of fore wing, deep and coarsely crenulate notauli, eye much longer than temple in dorsal view and second tergite about as long as third tergite or slightly longer, but differs by having the hind tibia yellowish-brown (largely blackish in H. verticalis), the third metasomal tergite (except basally) largely smooth and, in lateral view, truncated apically (coarsely rugose and rounded apically in H. verticalis), vein 1-r-m of hind wing shorter than vein 1-M (about of equal length in H. verticalis), the pterostigma subparallel-sided apically (slightly widened in H. verticalis), vein 3-CU1 of fore wing comparatively short (long in H. verticalis) and the precoxal sulcus wide medially (comparatively narrow in H. verticalis).
♀. Length of body in lateral view 2.6 mm, length of antenna 4.7 mm and length of fore wing 2.9 mm.
Colour. Body (Fig.
Mesosoma. Mesosoma (Fig.
Leg.
Hind coxa compressed, 1.5 times longer than hind trochanter; hind femur 0.7 times longer than hind tibia and 8.0 times longer than wide; hind tibia 1.1 times longer than hind tarsus, tarsal claws slender (Fig.
Metasoma. First tergite parallel-sided posteriorly, striate and 1.1 times longer than its apical width (Fig.
Hylcalosia bicolor sp. nov., ♀, holotype A body, lateral view B antennae C wings D head, dorsal view E head, ventral view F mesosoma, dorsal view G mesosoma, lateral view H propodeum and first metasomal tergite, dorsal view I ovipositor and hind leg, lateral view J mandible, lateral view K hind tarsus, dorsal view L hind tarsus, lateral view.
Unknown.
South Korea.
Hylcalosia sutchanica
Belokobylskij, 1992: 148; 1998: 298; 2015: 538; Fischer, 2008: 722;
Hylcalosia adsimilis
Papp, 1994: 139; Belokobylskij, 1998: 298; Fischer, 2008: 722; Yu et al., 2012;
2♀ (
♀, length of body in lateral view 2.6–2.7 mm, length of antenna 4.1–4.3 mm and length of fore wing 2.7–2.9 mm.
Colour. Body largely blackish; head entirely black dorsally and anteriorly brown, antenna reddish-brown and apically dark brown, mandible pale brown and apically dark brown; first tergite reddish-brown and mesonotum entirely blackish or dark brown.
Head. Head (Fig.
Hylcalosia sutchanica Belokobylskij, 1992 ♀ A body, lateral view B antennae C wings D head, dorsal view E head, ventral view F mesosoma, dorsal view G mesosoma, lateral view H propodeum and first metasomal tergite, dorsal view I ovipositor and hind leg, lateral view J mandible, lateral view K hind tarsus, dorsal view L hind tarsus, lateral view.
Mesosoma. Mesosoma (Fig.
Leg.
Hind coxa smooth and 1.2 times longer than hind trochanter; hind femur 0.9 times as long as hind tibia and 8.5 times longer than wide; hind tibia 0.9 times longer than hind tarsus, tarsal claws slender (Fig.
Metasoma. First tergite gradually widened posteriorly (Fig.
Russia, China, Korea.
This work was supported by the Korean National Arboretum (KNA1-1-20) and a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202130203).