Research Article |
Corresponding author: Daniel Winkler ( winklerdanielandras@gmail.com ) Academic editor: Louis Deharveng
© 2021 Daniel Winkler, Márton Tamás Németh, Cristina Fiera.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Winkler D, Németh MT, Fiera C (2021) A new species of Pseudosinella Schäffer, 1897 (Collembola, Entomobryidae) from Hungary and Romania, with redescription of the related species Pseudosinella huetheri Stomp, 1971. ZooKeys 1063: 121-137. https://doi.org/10.3897/zookeys.1063.73094
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A new species of the genus Pseudosinella Schäffer, 1897 from Hungary and Romania is described and illustrated. Pseudosinella hartnerae sp. nov. belongs to the group with 5+5 eyes, and can be identified by its unique labial chaetotaxy (M1m2rel1L2) within this group. Pseudosinella huetheri Stomp, 1971, the closest related species sharing the same dorsal macrochaetae formula (R0R1R2001/00/0101+2), is also redescribed here based on the holotype. Comparative analysis between the two species and among other related species is provided.
Chaetotaxy, Pseudosinella hartnerae sp. nov., springtails, taxonomy
Pseudosinella Schäffer, 1897 is the largest collembolan genus, represented by 382 described species worldwide (
As a part of soil biodiversity surveys in Hungary, the Bátorliget Pasture Nature Conservation Area was revisited in 2017. Among the collected Collembola material, a new species of Pseudosinella Schäffer, 1897 was discovered and is described in the present paper. Around the same time, Collembola samplings were carried out in Romania Mohoş Nature Reserve, from where the same Pseudosinella species was also found. The genus was previously represented by 17 species in Hungary (
The new species belongs to the group of species with 5+5 eyes. With the help of the computer assisted Delta identification key using the combination of chaetotaxic and other characters, originally designed by
In October 2017, soil samplings were carried out in the Bátorliget Pasture Nature Conservation Area (East Hungary). A month later, soil mesofauna samplings were carried out also in Romania, around St. Ana Lake (Mohoş Nature Reserve). Springtails were extracted from the hand collected litter and soil samples within 14 days using a modified Berlese–Tullgren apparatus (without light or heating devices). The specimens were cleared using Nesbitt fluid and then mounted on permanent slides in Hoyer’s medium. The slides were examined under a Leica DM2500 LED microscope with conventional bright light and phase contrast.
Abbreviations used in text and figures are:
Abd abdominal tergite;
accp accessorial p–sensilla;
Ant antennal segment;
a.s.l. above sea level;
NHMG Natural History Museum of Geneva;
Mac macrochaeta;
mic microchaeta;
psp pseudopore;
Th thoracic tergite.
Symbols used in figures:
open circle Mac;
black dots mic;
x trichobotria.
Dorsal head chaetotaxy follows
Order Entomobryomorpha Börner, 1913
Family Entomobryidae Schäffer, 1896
Subfamily Lepidocyrtinae Wahlgren, 1906
Holotype: Hungary. ♀ on slide (Nr.
Romania. Seven specimens (one ♂ on slide, Nr.:
5+5 ocelli. Colour bluish-grey. Labial chaetotaxy M1m2rel1L2, r vestigial. Dorsal macrochaetae formula R0R1R2001/00/0101+2. Abd II chaetotaxy: paBq1q2. Abd IV accessory chaeta s, anteriorly to trichobothrial complex, absent. Antennae and legs without scales. Unguis inner side with two paired basal teeth and one unpaired tooth. Unguiculus outer lamella smooth.
Habitus
(Fig.
Head. With 5+5 eyes (ABCDH, with H only slightly smaller). Dorsal cephalic main macrochaetae formula R0R1R2P (according to AMS notation A0, A2, A3 and Pa5) (Fig.
Pseudosinella hartnerae sp. nov. A head, dorsal chaetotaxy (left side) B antennae, Ant III with sensillar organ C clypeus, clypeal chaetae D labrum E labial papilla E with lateral process F labial triangle (right side) and ventral cephalic groove with labial and postlabial chaetotaxy. Scale bars: 0.05 mm (A); 0.03 mm (B–D, F); 0.01 mm (E).
Body. Body dorsal macrochaetae from Th II to Abd IV 00/0101+2. Mesothorax without macrochaetae. Two anterolateral S-chaetae (al and ms) present. Th III without Mac, anterolateral sensillum al present. Abd I with lateral S-microchaeta (ms). Chaetotaxy of Abd II–III as in Fig.
The type locality (Bátorliget Pasture Nature Conservation Area, Szabolcs–Szatmár–Bereg county, Hungary) of Pseudosinella hartnerae sp. nov. is a special relict mire and forest area with high biodiversity. The new species was collected from the upper layer and litter of a forest clearing with pioneer vegetation including silver birch (Betula pendula) trees. Specimens in Romania were collected from litter in the surrounding forest of the volcanic lake Saint Ana, and from peat mosses in the nearby Mohoș bog. Both Romanian sites are located in the Mohoş Nature Reserve, Harghita County. This new Pseudosinella is a phytodetriticolous, bryophilous and hygrophilous species.
The name of the new species is dedicated to former zoologist colleague and friend Dr. Anna Fenyősiné Hartner (1965–2006), an excellent specialist in myrmecology.
Holotype: Luxembourg. sex not visible, on slide, preserved in NHMG (Fig.
5+5 ocelli. Colour yellowish white. Labial chaetotaxy M1m2Rel1L2, R ~ 0.5 of M. Dorsal macrochaetae formula R0R1R2001/00/0101+2. Abdominal tergite II chaetotaxy: –aBq1q2. Abd IV accessory chaeta s, anteriorly to trichobothrial complex, absent. Antennae and legs without scales. Unguis inner side with two paired basal teeth and one unpaired tooth, unguiculus outer lamella smooth.
Habitus
(Fig.
Head. With 5+5 eyes (ABCDH, with H only slightly smaller) (Fig.
Body. Body dorsal macrochaetae from Th II to Abd IV 00/0101+2. Mesothorax without Mac. Two anterolateral S-chaetae (al and ms) present. Th III without Mac, anterolateral sensillum al present. Abd I with lateral S-microchaeta (ms). Chaetotaxy of Abd II–III as in Fig.
Pseudosinella huetheri was described from Luxembourg, from a beech forest near Berdorf (
Considering the number of eyes (5+5) and the similar dorsal main macrochaetae distribution (R000/00/0101+2 or R001/00/0101+2), Pseudosinella hartnerae sp. nov. is close to P. altamirensis Baquero, Jordana, Labrada & Luque, 2020; P. horaki Rusek, 1985; P. huetheri; P. mauli Stomp, 1972; P. mucronata Gouze & Deharveng, 1987; and P. sandelsorum Gruia, 1977 (Table
Comparison of P. hartnerae sp. nov. with related species with 5+5 eyes and similar dorsal macrochaetae distribution.
Species | Ch1 | Ch2 | Ch3 | Ch4 | Ch5 | Ch6 | Ch7 | Ch8 | Ch9 | Ch10 | Ch11 | Ch12 |
---|---|---|---|---|---|---|---|---|---|---|---|---|
P. altamirensis | pale with blue pigmentation | M1m2Rel1l2 | R000/00/0101+2 | 1.6–2.2 | – | + | 3 | 35% | 40% | 1 | 2+7–12 | 2 |
P. horaki | pale greyish blue | M1M2REL1L2 | R001/00/0101+2 | 1.6 | + | – | 3 | 50% | 75% | 2 | U | 1 |
P. huetheri | yellowish white | M1m2Rel1L2 | R001/00/0101+2 | 1.4 | – | – | 3 | 45% | 65% | 2 | 2+2 | 1 |
P. mauli | bluish black | M1M2rEL1L2 | R001/00/0101+2 | 1.3 | + | + | 3 | ~45% | 65% | 2 | 2+3 | 1 |
P. mucronata | diffuse pigmentation | m1(M1)m2Rel1l2 | R000/00/0101+2 | 1.8–2.0 | – | – | 3 | ~45% | 60% | 1 | U | 2 |
P. sandelsorum | dark blue pigment on Ant and legs | M1M2REL1L2 | R001/00/0101+2 | 1.3 | + | – | 4 | ~50% | 70% | 2 | 2+4–10 | 1 |
P. hartnerae sp. nov | bluish-grey | M1m2rel1L2 | R001/00/0101+2 | 1.2–1.4 | + | – | 3 | 35% | 60% | 2 | 2+2 | 1 |
We would like to thank Edit Horváth (