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Research Article
A new species of Cyrtodactylus Gray, 1827 (Squamata, Gekkonidae) from southwestern Yunnan, China
expand article infoShuo Liu‡, Dingqi Rao‡
‡ Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China

Corresponding author: Shuo Liu ( liushuo@mail.kiz.ac.cn )

Corresponding author: Dingqi Rao ( raodq@mail.kiz.ac.cn )

Academic editor: Thomas Ziegler
© 2022 Shuo Liu, Dingqi Rao.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Liu S, Rao D (2022) A new species of Cyrtodactylus Gray, 1827 (Squamata, Gekkonidae) from southwestern Yunnan, China. ZooKeys 1084: 83-100. https://doi.org/10.3897/zookeys.1084.72868
ZooBank: urn:lsid:zoobank.org:pub:670EC7CC-90D3-4B2E-8EDE-7C34BF70FA0A
Open Access

Abstract

A new species of the Cyrtodactylus chauquangensis species group is described based on four specimens collected from the karst formations of Menglian County, Puer City, Yunnan Province, China. The new species can be separated from all other congeners by having a unique combination of morphological characters: a medium-sized body; ventrolateral folds present with interspersed small tubercles; seven precloacal pores in a continuous series in males, absent in females; enlarged femoral scales and femoral pores absent; two postcloacal tubercles on each side; and one or two rows of enlarged subcaudals. Genetically, the new species most closely related to C. wayakonei and the uncorrected sequence divergences of the ND2 gene and its flanking tRNAs between the new species and investigated congeners range from 7.2% to 18.4%.

Keywords

Bent-toed gecko, Cyrtodactylus chauquangensis group, Menglian County, taxonomy

Introduction

Cyrtodactylus Gray, 1827 is the most speciose and ecologically diverse gekkotan genus with more than 300 recognized species so far (Grismer et al. 2021a, b; Uetz et al. 2021). Grismer et al. (2021a) partitioned the species of Cyrtodactylus into 10 ecotypes according to their habitat preferences. Of the 10 ecotypes, the karst ecotype is the second largest and contains the majority of the most recently described species (Grismer et al. 2021a).

The Cyrtodactylus chauquangensis Hoang, Orlov, Ananjeva, Johns, Hoang & Dau, 2007 species group, previously C. wayakonei Nguyen, Kingsada, Rösler, Auer & Ziegler, 2010 species group, is a karst ecotype species group, which is distributed in northern Indochina, ranging from northern Thailand and Laos to northwestern and central Vietnam, and to Yunnan Province in southern China (Grismer et al. 2021a, b). This species group contains 23 named species to date (Liu and Rao 2021a; Liu et al. 2021; Zhang et al. 2021).

During our fieldwork in southern Yunnan Province, China, in 2021, some specimens of Cyrtodactylus were collected from the karst formations of Menglian County, Puer City. Morphological and molecular phylogenetic analyses revealed that the new collection belonged to an unnamed species of the C. chauquangensis species group. We herein describe it as a new species.

Materials and methods

Sampling

Specimens were collected by hand. Photographs were taken to document the color pattern of specimens in life prior to their euthanization. Liver tissues were stored in 99% ethanol and specimens were preserved in 75% ethanol. Specimens were deposited at Kunming Natural History Museum of Zoology, Kunming Institute of Zoology, Chinese Academy of Sciences (KIZ).

Morphological analyses

Measurements were taken with digital calipers to the nearest 0.1 mm. Bilateral scale counts were given as left/right. The methodology of measurements and meristic counts followed Liu and Rao (2021a, b):

AG axilla to groin distance;

DTR dorsal tubercle rows, number of dorsal, longitudinal rows of tubercles at midbody between ventrolateral folds;

ED ear diameter, greatest diameter of ear;

EE eye orbit to ear distance, from posterior corner of eye orbit to anterior margin of ear opening;

EFS enlarged femoral scales, number of enlarged femoral scale beneath each thigh;

ForeaL forearm length, from base of palm to elbow;

FP femoral pores;

GSDT granular scales surrounding dorsal midbody tubercles;

HH maximum head height, from occiput to underside of jaws;

HL head length, from tip of snout to posterior margin of retroarticular of lower jaw;

HW maximum head width;

I postrostrals or internasals;

IFL infralabials;

IND internarial distance, measured between inner borders of nostrils;

IOD interorbital distance, measured across narrowest point of frontal bone;

LF4 subdigital lamellae under the fourth finger;

LT4 subdigital lamellae under the fourth toe;

ML mental length;

MW mental width;

OD greatest diameter of orbit;

PAT postcloacal tubercles, number of tubercles on each side of postcloacal region;

PM postmentals, scales bordering mental shield, except infralabials;

PP precloacal pores;

PVT paravertebral tubercles, counted in a single paravertebral row from the level of the forelimb insertions to the level of the hind limb insertion;

RH rostral height;

RW rostral width;

SE snout to eye distance, from tip of snout to anterior corner of eye orbit;

SL shank length, from the base of heel to the knee;

SPL supralabials;

SVL snout–vent length, from tip of snout to anterior margin of cloaca;

TaL tail length, from posterior margin of cloaca to tip of tail;

V longitudinal ventral scale rows, counted across the belly between the ventro­lateral folds at midbody.

Morphological comparisons were based on the original descriptions of each species (Hoang et al. 2007; Bauer et al. 2009, 2010; Ngo and Grismer 2010; Nguyen et al. 2010, 2015b, 2017; Sumontha et al. 2010; Luu et al. 2011; Ngo 2011; Ngo and Chan 2011; Kunya et al. 2014; Nazarov et al. 2014; Nguyen et al. 2014; Schneider et al. 2014, 2020; Le 2016; Pham et al. 2019; Liu and Rao 2021a; Liu et al. 2021; Zhang et al. 2021).

Molecular analyses

Molecular data were generated for three specimens collected from Menglian County, Puer City, Yunnan Province, China, and available sequences of the Cyrtodactylus chauquangensis species group were obtained from GenBank; the new sequences have been deposited on GenBank under the accessions OM296042–OM296044. Cyrtodactylus dattkyaikensis Grismer, Wood, Quah, Grismer, Thura, Oaks & Lin, 2020 and C. sinyineensis Grismer, Wood Jr, Thura, Zin, Quah, Murdoch, Grismer, Lin, Kyaw & Lwin, 2017 were used as the outgroups according to Liu et al. (2021). Total genomic DNA was extracted from liver tissue stored in 99% ethanol using a DNeasy blood and tissue kit, Qiagen (California, USA). A fragment of the NADH dehydrogenase subunit 2 (ND2) gene and its flanking tRNAs was amplified and sequenced using the primers L4437b and H5934 (Macey et al. 1997). The experiment protocols used in this study are the same as Liu et al. (2021). Sequences were edited and assembled using SeqMan in Lasergene 7.1 (DNASTAR Inc., Madison, WI, USA) and MEGA X (Kumar et al. 2018).

Sequences were aligned using ClustalW (Thompson et al. 1994) with default parameters. The best-fit substitution models were chosen using the Bayesian Information Criterion (BIC) in ModelFinder (Kalyaanamoorthy et al. 2017) for IQ-TREE and MrBayes, respectively. Maximum likelihood analysis was performed in IQ-TREE 1.6.12 (Nguyen et al. 2015a) used the TIM+F+I+G4 model for the first codon position, the second codon position, and the tRNAs; and the GTR+F+R2 model for the third codon position. One thousand bootstrap pseudoreplicates via the ultrafast bootstrap approximation algorithm were used to construct a final consensus tree. Nodes with ultrafast bootstrap values of 95 and above were considered significantly supported (Minh et al. 2013). Bayesian inference was performed in MrBayes 3.2.6 (Ronquist et al. 2012) used the GTR+F+I+G4 model of evolution for the first codon position, the second codon position, and the tRNAs; and GTR+F+G4 model for the third codon position. The chains were run for 1,000,000 generations and sampled every 100 generations. The first 25% of the sampled trees was discarded as burn-in and then the remaining trees were used to estimate Bayesian posterior probabilities. Nodes with Bayesian posterior probabilities of 0.95 or higher were considered well-supported (Huelsenbeck et al. 2001; Wilcox et al. 2002). Pairwise distances between species were calculated in MEGA X (Kumar et al. 2018).

Results

The obtained sequence alignment is 1397 bp in length. The sequences of the three specimens collected from Menglian County, Yunnan, China, were nested within the Cyrtodactylus chauquangensis species group and represented a distinct clade sister to C. wayakonei Nguyen, Kingsada, Rösler, Auer & Ziegler, 2010 with strong support (Fig. 1). The interspecific pairwise distances between the newly collected specimens and other members of C. chauquangensis species group ranged from 7.2% to 18.4% (Table 1).

Table 1.
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Mean uncorrected pairwise genetic distances (%) among the species of the Cyrtodactylus chauquangensis species group and outgroups based on the ND2 gene and its flanking tRNAs.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23
1 Cyrtodactylus menglianensis sp. nov.
2 Cyrtodactylus auribalteatus 12.2
3 Cyrtodactylus bichnganae 18.4 18.3
4 Cyrtodactylus bobrovi 15.0 13.9 19.7
5 Cyrtodactylus chauquangensis 12.6 13.3 18.1 8.6
6 Cyrtodactylus cucphuongensis 15.0 14.5 19.9 7.9 8.4
7 Cyrtodactylus doisuthep 15.5 13.6 16.6 15.9 14.4 15.9
8 Cyrtodactylus dumnuii 11.4 11.9 17.0 13.7 12.3 14.4 14.3
9 Cyrtodactylus erythrops 13.9 13.8 16.7 14.8 13.5 14.7 11.0 13.4
10 Cyrtodactylus gulinqingensis 14.3 13.2 18.1 13.8 14.0 14.0 14.1 12.9 13.8
11 Cyrtodactylus houaphanensis 14.8 14.6 19.4 6.5 9.0 7.5 15.5 14.2 14.9 14.1
12 Cyrtodactylus huongsonensis 14.3 13.4 17.7 14.3 12.5 14.3 14.7 13.9 14.3 12.4 14.7
13 Cyrtodactylus ngoiensis 13.1 13.2 18.2 11.1 10.5 10.7 14.9 12.0 14.2 13.1 11.3 13.1
14 Cyrtodactylus otai 15.2 14.6 19.1 3.6 9.1 8.4 16.3 15.6 16.4 15.6 6.8 14.7 12.2
15 Cyrtodactylus puhuensis 14.2 13.4 18.9 5.7 8.0 7.1 14.7 12.9 14.2 13.6 2.8 13.8 10.5 6.2
16 Cyrtodactylus soni 13.4 13.0 18.2 14.3 13.6 14.4 14.2 13.1 14.2 13.0 15.3 6.7 14.0 14.7 14.2
17 Cyrtodactylus sonlaensis 17.2 16.2 19.4 17.5 16.8 18.1 16.2 16.8 17.3 14.8 18.0 15.0 16.2 17.7 18.0 15.2
18 Cyrtodactylus spelaeus 14.6 13.9 18.3 10.0 9.2 10.4 15.7 13.4 15.0 13.9 10.4 14.3 11.1 11.3 9.1 14.3 17.7
19 Cyrtodactylus taybacensis 16.8 15.6 9.3 17.1 15.5 17.3 15.7 14.9 16.4 16.3 17.3 16.1 16.6 18.3 16.7 15.6 18.9 16.5
20 Cyrtodactylus vilaphongi 14.1 13.4 17.8 8.1 7.3 8.2 14.2 13.3 14.0 13.5 8.2 14.2 9.5 9.1 7.0 13.5 16.9 9.6 16.5
21 Cyrtodactylus wayakonei 7.2 13.1 18.0 15.5 13.1 15.5 16.3 12.7 15.6 15.3 14.7 15.1 12.2 15.4 14.2 13.9 16.4 15.2 17.5 13.7
22 Cyrtodactylus zhenkangensis 10.7 12.0 18.4 14.1 13.2 13.8 15.5 11.8 14.0 12.9 13.9 13.4 13.2 15.5 13.2 13.7 17.3 14.0 15.8 13.6 11.9
23 Cyrtodactylus dattkyaikensis 18.3 17.2 21.4 18.8 18.0 19.4 18.0 17.0 16.8 16.8 19.9 17.2 18.0 21.5 18.8 17.9 21.8 19.3 18.4 17.8 19.9 17.9
24 Cyrtodactylus sinyineensis 18.8 17.8 18.9 18.7 17.5 18.1 18.2 17.0 18.7 18.9 18.9 19.1 17.5 20.9 18.1 18.7 21.9 18.9 17.9 18.1 19.4 18.4 13.1
Figure 1. 

Bayesian phylogram of the Cyrtodactylus chauquangensis species group inferred from the ND2 gene and its flanking tRNAs. Numbers before slashes indicate Bayesian posterior probabilities and numbers after slashes indicate the ML ultrafast bootstrap. The symbol “–” represents the value below 0.90/90.

Cyrtodactylus menglianensis sp. nov.

http://zoobank.org/6E8C0453-145B-4862-9A87-BC3F4F4344FD
Figures 2, 3, 4, 5

Type material

Holotype. KIZ20210713, adult male, collected on 18 July 2021 by Shuo Liu from Menglian County, Puer City, Yunnan Province, China (22°20'11"N, 99°34'29"E, 980 m elevation).

Paratypes. KIZ20210714 and KIZ20210716, two adult females; KIZ20210715, adult male; all collected on 19 July 2021 by Shuo Liu from the same locality as the holotype.

Figure 2. 

Type series of Cyrtodactylus menglianensis sp. nov. in preservative A dorsal views B ventral views.

Etymology

The specific epithet refers to Menglian County, the locality where the new species was found. We propose “Menglian Bent-toed Gecko” for the common English name and “孟连裸趾虎” (Mèng Lián Luǒ Zhǐ Hǔ) for the common Chinese name of the new species.

Figure 3. 

Close-up views of the femoral and precloacal regions of Cyrtodactylus menglianensis sp. nov. A male holotype (KIZ20210713) B female paratype (KIZ20210714).

Diagnosis

Medium body size (SVL 67.7–78.1 mm in adults); ventrolateral folds present with interspersed small tubercles; seven precloacal pores in a continuous series in males, absent in females; femoral scales not enlarged; femoral pores absent; two postcloacal tubercles on each side; 17–22 lamellae under finger IV, 21–23 lamellae under toe IV; one or two rows of subcaudals enlarged; dark postocular streak and nuchal loop absent; six or seven dark irregular dorsal bands between limb insertions, most bands discontinuous.

Figure 4. 

Holotype (KIZ20210713) of Cyrtodactylus menglianensis sp. nov. in life A dorsal view of whole body B lateral view of whole body C ventral view of whole body D dorsal view of head E lateral view of head F ventral view of head.

Description of holotype

Adult male, SVL 77.8 mm; head distinguished from neck, moderately long (HL/SVL 0.28), relatively widened (HW/HL 0.76), slightly depressed (HH/HL 0.45); two supranasals separated by one internasal; nares oval, surrounded by supranasal, rostral, first supralabial, and three postnasals; loreal region concave; snout long (SE/HL 0.43), round anteriorly, longer than diameter of orbit (OD/SE 0.63); snout scales small, round, granular, larger than those in frontal and parietal regions; eye large (OD/HL 0.27), pupils vertical; upper eyelid fringe with spinous scales; ear opening oval, small (ED/HL 0.09); rostral wider than high (RH/RW 0.58), medially divided dorsally by a suture, reaching to approximately half down rostral, in contact with first supralabial and nostrils laterally, and supranasals and internasal dorsally; mental triangular, narrower than rostral (MW/RW 0.82), slightly wider than high (ML/MW 0.94); two postmentals, enlarged, in contact posteriorly, bordered by mental anteromedially, first infralabial anterolaterally, two enlarged chin scales posterolaterally, and small chin scales posteriorly; 10/12 supralabials; 9/9 infralabials.

Figure 5. 

Paratypes of Cyrtodactylus menglianensis sp. nov. in life A female paratype (KIZ20210714) B male paratype (KIZ20210715) C female paratype (KIZ20210716).

Body slender (AG/SVL 0.39), ventrolateral folds slightly developed with interspersed small tubercles; dorsal scales granular; dorsal tubercles round and weakly keeled, four or five times larger than the size of adjoining scales, conical, present on neck, back and tail base, each surrounded by 10 granular scales, in 19 irregular longitudinal rows at the midbody, 26 paravertebral tubercles; gular region with homogenous smooth scales; ventral scales smooth, larger than those of dorsum, round, subimbricate, largest posteriorly, in 29 longitudinal rows at midbody; precloacal groove absent; a patch of precloacal scales significantly enlarged; seven precloacal pores in a continuous series, the two on the edge round, the one in the middle pitted, others horizontally elongated; femoral scales not enlarged; femoral pore absent.

Fore and hind limbs moderately slender (ForeaL/SVL 0.17, SL/SVL 0.20); dorsal surface of forelimbs covered by a few weakly developed tubercles; interdigital webbing absent; lamellae under finger IV 22/21, under toe IV 23/22; relative length of fingers I < II < V < III < IV, relative length of toes I < II < III < V < IV.

Tail regenerated (TaL 60.8 mm); 2/2 postcloacal tubercles; dorsal tail base with tubercles; subcaudals smooth, enlarged but arranged irregularly.

Color of holotype in life

Dorsal ground color brownish yellow; dorsal surface of head with irregular brown blotches with black edges, largest at occiput; nuchal loop absent; dorsum with many irregular brownish black blotches with black edges, forming eight transverse discontinuous bands faintly, one on the neck, one between hind limbs, and six between fore and hind limb insertions; dorsal surfaces of limbs with brown bands with black edges; a brown band with black edge on dorsal tail base, dorsal surface of regenerated tail greyish black; ventral surface of head, limbs, and body greyish white; ventral surface of regenerated tail grey; iris bronze.

Color of holotype in preservative

The color pattern very much resembles that in life. Brownish yellow dorsal ground color turned to greyish white, the brown blotches and bands with black edges remained; ventral surface faded to pale white; iris became white.

Variations

The paratypes resemble the holotype except that the female KIZ20210714 has a longer regenerated tail, and the female KIZ20210716 has a longer original tail with one or two rows of subcaudals enlarged, and they both have no precloacal pores; the male KIZ20210715 has a smaller body size and much shorter regenerated tail; other morphometric and meristic differences are presented in Table 2. Color patterns of the paratypes also resemble the holotype except that the dark bands on the dorsum are relatively more distinct, and there are 10 black and white rings on the original tail of the female KIZ20210716.

Table 2.
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Measurements (mm) and meristic data for the type series of Cyrtodactylus menglianensis sp. nov. Abbreviations defined in Materials and methods. “*” represents regenerated tail and “#” represents original tail.

KIZ20210713 Holotype KIZ20210714 Paratype KIZ20210715 Paratype KIZ20210716 Paratype
Male Female Male Female
SVL 77.8 78.1 69.1 67.7
TaL 60.8* 62.9* 33.2* 70.6#
HH 9.7 9.7 8.3 8.7
HL 21.7 21.8 19.4 19.2
HW 16.4 15.8 14.8 14.5
OD 5.9 6.9 5.1 5.3
SE 9.3 9.2 8.2 8.3
EE 6.3 5.9 5.6 5.6
IND 3.1 3.1 2.9 2.6
IOD 3.3 3.5 2.8 2.7
ED 1.9 1.3 1.3 1.2
AG 30.5 34.6 27.8 28.4
ForeaL 13.0 12.9 11.4 10.6
SL 15.7 15.6 14.0 13.1
RW 3.8 3.4 2.9 3.0
RH 2.2 2.1 1.9 1.8
MW 3.1 3.2 2.9 3.8
ML 2.9 2.6 2.6 2.0
SPL 10/12 9/10 8/9 10/9
IFL 9/9 9/9 9/8 9/7
I 1 1 1 1
PM 2 2 2 2
GSDT 10 10 10 10
DTR 19 21 20 18
PVT 26 27 25 29
V 29 26 26 28
EFS 0 0 0 0
PP 7 0 7 0
FP 0 0 0 0
PAT 2/2 2/2 2/2 2/2
LF4 22/21 19/18 18/17 21/22
LT4 23/22 22/22 22/21 23/23

Distribution

The new species is currently known only from the type locality (Fig. 6) in Menglian County, Puer City, Yunnan Province, China.

Figure 6. 

Map showing the type locality (red dot) of Cyrtodactylus menglianensis sp. nov. in Menglian County, Puer City, Yunnan Province, China.

Natural history

All specimens were collected at night on large stones or cliffs of the karst formations in a park. The surrounding habitats was karst forest, there is a plank road and a river nearby.

Comparisons

Cyrtodactylus menglianensis sp. nov. is distinguishable from all other members of the C. chauquangensis species group by a unique combination of morphological characters. Cyrtodactylus menglianensis sp. nov. differs from C. auribalteatus Sumontha, Panitvong & Deein, 2010; C. bichnganae Ngo & Grismer, 2010; C. doisuthep Kunya, Panmongkol, Pauwels, Sumontha, Meewasana, Bunkhwamdi & Dangsri, 2014; C. dumnuii Bauer, Kunya, Sumontha, Niyomwan, Pauwels, Chanhome & Kunya, 2010; C. erythrops Bauer, Kunya, Sumontha, Niyomwan, Panitvong, Pauwels, Chanhome & Kunya, 2009; C. gulinqingensis Liu, Li, Hou, Orlov & Ananjeva, 2021; C. hekouensis Zhang, Liu, Bernstein, Wang & Yuan, 2021; C. huongsonensis Luu, Nguyen, Do & Ziegler, 2011; C. ngoiensis Schneider, Luu, Sitthivong, Teynié, Le, Nguyen & Ziegler, 2020; C. soni Le, Nguyen, Le & Ziegler, 2016; C. sonlaensis Nguyen, Pham, Ziegler, Ngo & Le, 2017; and C. zhenkangensis Liu & Rao, 2021 by not having enlarged femoral scales and femoral pores (vs having enlarged femoral scales and femoral pores).

Cyrtodactylus menglianensis sp. nov. differs from C. puhuensis Nguyen, Yang, Le, Nguyen, Orlov, Hoang, Nguyen, Jin, Rao, Hoang, Che, Murphy & Zhang, 2014 and C. taybacensis Pham, Le, Ngo, Ziegler & Nguyen, 2019 by not having enlarged femoral scales (vs having enlarged femoral scales). In addition, C. menglianensis sp. nov. differs from C. puhuensis by having more precloacal pores in males (seven vs five) and differs from C. taybacensis by having fewer precloacal pores in males (seven vs 11–13).

Cyrtodactylus menglianensis sp. nov. differs from C. cucphuongensis Ngo & Chan, 2011 by having precloacal pores in males (vs not having precloacal pores in males).

Cyrtodactylus menglianensis sp. nov. differs from C. bobrovi Nguyen, Le, Pham, Ngo, Hoang, Pham & Ziegler, 2015; C. chauquangensis Hoang, Orlov, Ananjeva, Johns, Hoang & Dau, 2007; C. houaphanensis Schneider, Luu, Sitthivong, Teynié, Le, Nguyen & Ziegler, 2020; C. otai Nguyen, Le, Pham, Ngo, Hoang, Pham & Ziegler, 2015; C. spelaeus Nazarov, Poyarkov, Orlov, Nguyen, Milto, Martynov, Konstantinov & Chulisov, 2014; and C. vilaphongi Schneider, Nguyen, Le, Nophaseud, Bonkowski & Ziegler, 2014 by not having dark postocular streak and nuchal loop (vs having very obvious dark postocular streak and not obvious nuchal loop).

Cyrtodactylus menglianensis sp. nov. differs from C. martini Ngo, 2011 by not having enlarged femoral scales (vs having indistinctly enlarged femoral scales), having fewer longitudinal ventral scale rows (26–29 vs 39–43), having more precloacal pores in males (seven vs four), and having more white rings on the original tail (10 vs 7).

Cyrtodactylus menglianensis sp. nov. differs from C. wayakonei Nguyen, Kingsada, Rösler, Auer & Ziegler, 2010 by having fewer longitudinal ventral scale rows (26–29 vs 31–35), not having precloacal pores in females (vs having precloacal pores in females), and having more white rings on the original tail (10 vs 6).

Discussion

The new species was found in a park just beside the county seat. There is a plank road along the limestone cliffs in the park, and there are many lamps on the limestone cliffs along the plank road (Fig. 7). These lamps light up every night and have some influence on nocturnal animals. We found that the populations of nocturnal animals there were very small, including that of the new species. Next to the park is a small nature reserve, which focuses only on the protection of several rare plants. We suggest that this nature reserve also include animals and the karst formations into their protection.

Figure 7. 

Habitat of Cyrtodactylus menglianensis sp. nov. at the type locality A distant view B close view C, D lamps on the limestone cliffs.

There are still many karst landforms in southern Yunnan which have not been surveyed in detail. Additional cryptic new species of Cyrtodactylus are likely to be found in these areas. It is necessary to strengthen the protection of these karst landforms and to survey these areas.

Acknowledgements

We thank to our workmates for their help and advice. We also thank the editors and reviewers for their comments on the manuscript. This work was supported by Science-Technology Basic Condition Platform from the Ministry of Science and Technology of the People’s Republic of China (grant no. 2005DKA21402), National Natural Science Foundation Project: Investigation and Classificatory and Phylogenetic Studies on the Lizards of Gekkonidae of China (grant no. NSFC-31970404), and the project of Ministry of Ecology and Environment of China: Investigation and assessment of amphibians and reptiles in southern Yunnan.

References

  • Bauer AM, Kunya K, Sumontha M, Niyomwan P, Panitvong N, Pauwels OSG, Chanhome L, Kunya T (2009) Cyrtodactylus erythrops (Squamata: Gekkonidae), a new cave-dwelling gecko from Mae Hong Son Province, Thailand. Zootaxa 2142: 51–62. https://doi.org/10.11646/zootaxa.2124.1.4
  • Bauer AM, Kunya K, Sumontha M, Niyomwan P, Panitvong N, Pauwels OSG, Chanhome L, Kunya T (2010) Cyrtodactylus dumnuii (Squamata: Gekkonidae), a new cave-dwelling gecko from Chiang Mai Province, Thailand. Zootaxa 2570: 41–50. https://doi.org/10.11646/zootaxa.2570.1.2
  • Dring JCM (1979) Amphibians and reptiles from northern Trengganu, Malaysia, with descriptions of two new geckos: Cnemaspis and Cyrtodactylus. Bulletin of the British Museum (Natural History), Zoology 34: 181–241.
  • Grismer LL, Wood Jr PL, Thura MK, Thaw Zin, Quah ESH, Murdoch ML, Grismer MS, Lin A, Kyaw H, Lwin N (2017) Twelve new species of Cyrtodactylus Gray (Squamata: Gekkonidae) from isolated limestone habitats in east-central and southern Myanmar demonstrate high localized diversity and unprecedented microendemism. Zoological Journal of the Linnean Society 182(4): 862–959. https://doi.org/10.1093/zoolinnean/zlx057
  • Grismer LL, Wood Jr PL, Quah ESH, Grismer MS, Thura MK, Oaks JR, Lin A (2020) Two new species of Cyrtodactylus Gray, 1827 (Squamata: Gekkonidae) from a karstic archipelago in the Salween Basin of southern Myanmar (Burma). Zootaxa 4718(2): 151–183. https://doi.org/10.11646/zootaxa.4718.2.1
  • Grismer LL, Wood Jr PL, Poyarkov NA, Le MD, Karunarathna S, Chomdej S, Suwannapoom C, Qi S, Liu S, Che J, Quah ESH, Kraus F, Oliver PM, Riyanto A, Pauwels OSG, Grismer JL (2021a) Karstic landscapes are foci of species diversity in the world’s third-largest vertebrate genus Cyrtodactylus Gray, 1827 (Reptilia: Squamata; Gekkonidae). Diversity 13(5): e183. https://doi.org/10.3390/d13050183
  • Gray JE (1827) A synopsis of the genera of saurian reptiles in which some new genera are indicated, and the others reviewed by actual examination. The Philosophical Magazine 2(7): 54–58. https://doi.org/10.1080/14786442708675620
  • Grismer LL, Wood Jr PL, Poyarkov NA, Le MD, Kraus F, Agarwal I, Oliver PM, Nguyen SN, Nguyen TQ, Karunarathna S, Welton LJ, Stuart BL, Luu VQ, Bauer AM, O’Connell KA, Quah ESH, Chan KO, Ziegler T, Ngo H, Nazarov RA, Aowphol A, Chomdej S, Suwannapoom C, Siler CD, Anuar S, Ngo TV, Grismer JL (2021b) Phylogenetic partitioning of the third-largest vertebrate genus in the world, Cyrtodactylus Gray, 1827 (Reptilia; Squamata; Gekkonidae) and its relevance to taxonomy and conservation. Vertebrate Zoology 71: 101–154. https://doi.org/10.3897/vz.71.e59307
  • Hoang QX, Orlov NL, Ananjeva NB, Johns AG, Hoang TN, Dau VQ (2007) Description of a new species of the genus Cyrtodactylus Gray, 1827 (Squamata: Sauria: Gekkonidae) from the karst of North Central Vietnam. Russian Journal of Herpetology 14: 98–106. https://doi.org/10.30906/1026-2296-2007-14-2-98-106
  • Huelsenbeck JP, Ronquist F, Nielsen R, Bollback JP (2001) Bayesian inference of phylogeny and its impact on evolutionary biology. Science 294(5550): 2310–2314. https://doi.org/10.1126/science.1065889
  • Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nature Methods 14: 587–589. https://doi.org/10.1038/nmeth.4285
  • Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35: 1547–1549. https://doi.org/10.1093/molbev/msy096
  • Kunya K, Panmongkol A, Pauwels OGS, Sumontha M, Meewasana J, Bunkhwamdi W, Dangsri S (2014) A new forest-dwelling bent-toed gecko (Squamata: Gekkonidae: Cyrtodactylus) from Doi Suthep, Chiang Mai Province, northern Thailand. Zootaxa 3811: 251–261. https://doi.org/10.11646/zootaxa.3811.2.6
  • Luu VQ, Nguyen TQ, Do HQ, Ziegler T (2011) A new Cyrtodactylus (Squamata: Gekkonidae) from Huong Son limestone forest, Hanoi, northern Vietnam. Zootaxa 3129: 39–50. https://doi.org/10.11646/zootaxa.3129.1.3
  • Minh Q, Nguyen MAT, von Haeseler A (2013) Ultrafast approximation for phylogenetic bootstrap. Molecular Biology and Evolution 30(5): 1188–1195. https://doi.org/10.1093/molbev/mst024
  • Nazarov RA, Poyarkov NA, Orlov NL, Nguyen SN, Milto KD, Martynov AA, Konstantinov EL, Chulisov AS (2014) A review of genus Cyrtodactylus (Reptilia: Sauria: Gekkonidae) in fauna of Laos with description of four new species. Proceedings of the Zoological Institute RAS 318: 391–423.
  • Ngo TV (2011) Cyrtodactylus martini, another new karst-dwelling Cyrtodactylus Gray, 1827 (Squamata: Gekkonidae) from Northwestern Vietnam. Zootaxa 2834: 33–46. https://doi.org/10.11646/zootaxa.2834.1.3
  • Ngo TV, Grismer LL (2010) A new karst dwelling Cyrtodactylus (Squamata: Gekkonidae) from Son La Province, northwestern Vietnam. Hamadryad 35: 84–95.
  • Nguyen LT, Schmidt HA, von Haeseler A, Minh BQ (2015a) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-like­lihood phylogenies. Molecular Biology and Evolution 32: 268–274. https://doi.org/10.1093/molbev/msu300
  • Nguyen TQ, Le MD, Pham AV, Ngo HN, Hoang CV, Pham CT, Ziegler T (2015b) Two new species of Cyrtodactylus (Squamata: Gekkonidae) from the karst forest of Hoa Binh Province, Vietnam. Zootaxa 3985(3): 375–390. https://doi.org/10.11646/zootaxa.3985.3.3
  • Nguyen TQ, Pham AV, Ziegler T, Ngo HT, Le MD (2017) A new species of Cyrtodactylus (Squamata: Gekkonidae) and the first record of C. otai from Son La Province, Vietnam. Zootaxa 4341(1): 25–40. https://doi.org/10.11646/zootaxa.4341.1.2
  • Nguyen SN, Yang JX, Le NT, Nguyen LT, Orlov NL, Hoang CV, Nguyen TQ, Jin JQ, Rao DQ, Hoang TN, Che J, Murphy RW, Zhang YP (2014) DNA barcoding of Vietnamese bent-toed geckos (Squamata: Gekkonidae: Cyrtodactylus) and the description of a new species. Zootaxa 3784(1): 48–66. https://doi.org/10.11646/zootaxa.3784.1.2
  • Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
  • Schneider N, Luu VQ, Sitthivong S, Teynié A, Le MD, Nguyen TQ, Ziegler T (2020) Two new species of Cyrtodactylus (Squamata: Gekkonidae) from northern Laos, including new finding and expanded diagnosis of C. bansocensis. Zootaxa 4822(4): 503–530. https://doi.org/10.11646/zootaxa.4822.4.3
  • Schneider N, Nguyen TQ, Le MD, Nophaseud L, Bonkowski M, Ziegler T (2014) A new species of Cyrtodactylus (Squamata: Gekkonidae) from the karst forest of northern Laos. Zootaxa 3835(1): 80–96. https://doi.org/10.11646/zootaxa.3835.1.4
  • Sumontha M, Panitvong N, Deein G (2010) Cyrtodactylus auribalteatus (Squamata: Gekkonidae), a new cave-dwelling gecko from Phitsanulok Province, Thailand. Zootaxa 2370: 53–64. https://doi.org/10.11646/zootaxa.2370.1.3
  • Termprayoon K, Rujirawan A, Grismer LL, Wood Jr PL, Aowphol A (2021) Taxonomic reassessment and phylogenetic placement of Cyrtodactylus phuketensis (Reptilia, Gekkonidae) based on morphological and molecular evidence. ZooKeys 1040: 91–121. https://doi.org/10.3897/zookeys.1040.65750
  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673–4680. https://doi.org/10.1093/nar/22.22.4673
  • Wilcox TP, Zwickl DJ, Heath TA, Hillis DM (2002) Phylogenetic relationships of the Dwarf Boas and a comparison of Bayesian and bootstrap measures of phylogenetic support. Molecular Phylogenetics and Evolution 25: 361–371. https://doi.org/10.1016/S1055-7903(02)00244-0
  • Zhang YP, Liu XL, Bernstein J, Wang J, Yuan ZY (2021) A New Species of Cyrtodactylus (Squamata: Gekkonidae) from the Karst Forests of Daweishan National Nature Reserve, Yunnan, China. Asian Herpetological Research 12(3): 1–11. https://doi.org/10.16373/j.cnki.ahr.200090
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