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Research Article
New species of the genus Inversidens Haas, 1911 (Unionoida, Unionidae, Gonideinae) from Jiangxi Province, China
expand article infoRuiwen Wu‡, Xiongjun Liu§, Takaki Kondo|, Shan Ouyang, Xiaoping Wu
‡ Shanxi Normal University, Linfen, China
§ Jiaying University, Meizhou, China
| Osaka Kyoiku University, Osaka, Japan
¶ Nanchang University, Nanchang, China

Corresponding author: Xiongjun Liu ( 609449126@qq.com )

Corresponding author: Xiaoping Wu ( xpwu@ncu.edu.cn )

Academic editor: Graham Oliver
© 2021 Ruiwen Wu, Xiongjun Liu, Takaki Kondo, Shan Ouyang, Xiaoping Wu.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Wu R, Liu X, Kondo T, Ouyang S, Wu X (2021) New species of the genus Inversidens Haas, 1911 (Unionoida, Unionidae, Gonideinae) from Jiangxi Province, China. ZooKeys 1054: 85-93. https://doi.org/10.3897/zookeys.1054.69075
ZooBank: urn:lsid:zoobank.org:pub:BA743F4E-2452-432A-9796-E1DEBC1D4CAD
Open Access

Abstract

We diagnose and describe a new freshwater mussel species of the genus Inversidens, I. rentianensis sp. nov. from Jiangxi Province, China based on morphological characters and molecular data. This paper includes a morphological description and photograph of the holotype, and partial sequences of mitochondrial COI as DNA barcode data.

Keywords

COI, freshwater mussel, genetic distances, morphology, taxonomy

Introduction

The genus Inversidens Haas, 1911 belongs to the subfamily Gonideinae in the family Unionidae. The genus was first depicted by Haas (1911) as a subgenus of Nodularia with two species, i.e., Unio brandtii Kobelt, 1879 and Nodularia parcedentata Haas, 1911, both restricted to Japan. Later, Haas (1969) further classified Unio reinianus Kobelt, 1879, Unio haconensis Ihering, 1893, Unio japanensis Lea, 1859, Unio pantoensis Neumayr, 1899 within Inversidens. All species were restricted to Japan, except for U. pantoensis, which was distributed in China. By comparing the conchological characters, Kondo (1982) believed that U. brandtii was not morphologically distinct from N. parcedentata, and regarded N. parcedentata as a variety of U. brandtii. Based on the morphology of the glochidium, Habe (1991) removed U. reinianus from Inversidens, and established a new genus Inversiunio. Based on morphological characteristics of the shell, Kondo (1998) moved Unio haconensis, which was regarded as a synonym of Unio jokohamensis (Ihering, 1893), into Inversiunio. Furthermore, Starobogatov (1970) used Unio japanensis as the type species for his newly established genus Pronodularia.

Currently, only two species are recognized within Inversidens, the Japanese endemic I. brandtii and I. pantoensis in China (Fig. 1A, B; Kondo 2008; He and Zhuang 2013; Lopes-Lima et al. 2020; Graf and Cummings 2021a, b; MolluscaBase eds. 2021).

Figure 1. 

Photographs of Inversidens taxa A I. brandtii B I. pantoensis C I. rentianensis sp. nov. Photos: [A, B] from the MUSSEL Project, [C] from this study, NCFM180325 (holotype), scale is 2 cm.

In this study, we diagnose and describe a new Inversidens species from Jiangxi Province, China. In addition, we provide estimations of the intraspecific and interspecific genetic distances within Inversidens based on the mitochondrial COI barcode to examine species validity.

Materials and methods

Specimen collection and identification

In March 2018, four samples were collected from the Mianshui River, Rentian Town, Ganzhou City, Jiangxi Province, China (25.989557°N, 116.131333°E). All type and voucher specimens are deposited at the Biological Museum of Nanchang University, China (NCFM180325-NCFM180328).

DNA extraction and COI amplification

Of the four individuals, only two samples had tissues. Total genomic DNA was extracted from dissected somatic tissue using TIANamp Marine Animals DNA Kit (Tiangen Biotech, Beijing, China) according to the manufacturer’s instructions.

Mitochondrial cytochrome oxidase subunit I (COI) gene sequences have been widely used for species delimitation of freshwater mussels based on genetic distance and the criteria of monophyly (Elderkin et al. 2016; Lopes-Lima et al. 2019; Smith et al. 2019). Polymerase chain reaction (PCR) amplification of the COI gene with a 680-base pair fragment was performed using a primer pair consisting of LCO1490 and HCO2198 (Folmer et al. 1994). Thermal cycling conditions were 98 °C for 10 s, followed by 35 cycles of 94 °C for 1 min, 50 °C for 1 min, 72 °C for 1–2 min, and a final extension of 72 °C for 7 min, following the TaKaRa Ex manufacturer’s protocol. The amplified PCR products were purified and sequenced by Sangon Biotech (Shanghai). The PCR product size for the COI amplicon was 680 bp. The sequences obtained in this study have been uploaded to GenBank.

DNA barcode dataset construction

We constructed a mitochondrial COI dataset with the newly obtained sequences from this study and the available Inversidens brandtii sequences from GenBank.

Previously published sequences were downloaded from GenBank and added to the dataset, i.e., 17 species of the subfamily Gonideinae and four species of the subfamily Unioninae for the ingroup, and one species of the subfamily Parreysiinae as the outgroup.

As a result, a total of 29 COI sequences were used for this study. Sequence details and GenBank accession numbers are shown in Table 1.

Table 1.
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List of sequences used in this study. (*) Sequenced from this study.

Taxa GenBank accession number
UNIONIDAE Rafinesque, 1820
Parreysiinae Henderson, 1935
Indonaia andersoniana (Nevill, 1877) KX865835
Unioninae Rafinesque, 1820
Acuticosta chinensis (Lea, 1868) MG462919
Inversiunio jokohamensis (Ihering, 1893) LC518985
Inversiunio reinianus (Kobelt, 1879) LC518976
Nodularia douglasiae (Griffith & Pidgeon, 1833) NC_026111
Gonideinae Ortmann, 1916
Pseudodon bogani Bolotov, Kondakov & Konopleva in Bolotov et al. 2017 MF352216
Pseudodon manueli Konopleva, Kondakov & Vikrev in Bolotov et al. 2017 MF352228
Monodontina cambodjensis (Petit de la Saussaye, 1865) KP795028
Pilsbryoconcha exilis (Lea, 1838) KP795024
Chamberlainia hainesiana (Lea, 1856) KX822635
Sinohyriopsis cumingii (Lea, 1852) NC_011763
Sinohyriopsis schlegelii (Martens, 1861) NC_015110
Lamprotula caveata (Heude, 1877) KX822646
Lamprotula leaii (Griffith & Pidgeon, 1833) NC_023346
Potomida littoralis (Cuvier, 1798) JN243905
Pronodularia japanensis (Lea, 1859) KX822659
Gonidea angulata (Lea, 1838) DQ272371
Leguminaia wheatleyi (Lea, 1862) KX822651
Microcondylaea bonellii (Férussac, 1827) KX822652
Sinosolenaia carinata (Heude, 1877) KX822669
Ptychorhynchus pfisteri (Heude, 1874) KY067440
Parvasolenaia rivularis (Heude, 1877) KX966393
Inversidens brandtii (Kobelt, 1879) AB040827
Inversidens brandtii (Kobelt, 1879) MT020598
Inversidens brandtii (Kobelt, 1879) MT020597
Inversidens brandtii (Kobelt, 1879) LC519005
Inversidens brandtii (Kobelt, 1879) LC519004
Inversidens rentianensis sp. nov. 1* MZ073336
Inversidens rentianensis sp. nov. 2* MZ073337

All COI nucleotide sequences were translated to amino acid sequences using MEGA 5.0 (Tamura et al. 2011) and aligned based on the amino acid sequences using the program MUSCLE (Edgar 2004) with default settings. We calculated and compared inter-and intraspecific distances with MEGA 5.0 using the uncorrected p-distance. Standard error was assessed using 1000 bootstrap replicates.

Phylogenetic analysis

Bayesian inference (BI) analyses were inferred in MrBayes Version 2.01 (Ronquist et al. 2012), using GTRGAMMAI model of nucleotide substitution. Four chains were run simultaneously for 10 million generations and trees were sampled every 1000 generations. The first 25% of these trees were discarded as burn-in when computing the consensus tree (50% Majority Rule). Sufficient mixing of the chains was considered to have been reached when the average standard deviation of split frequencies was below 0.01. Additionally, IQ-TREE was run for Maximum Likelihood (ML) tree reconstruction, using partition models with 1000 ultrafast bootstraps (Minh et al. 2013).

Taxonomy

Inversidens rentianensis Wu & Wu, sp. nov.

http://zoobank.org/62424717-9514-4C7D-9C0E-240F1D95F03E
Fig. 1C

Type specimens

Holotype. China • Jiangxi Province, Ganzhou City, Rentian Town (壬田镇), Mianshui River (25.989557°N, 116.131333°E), 13 March 2018, coll. Xiongjun Liu (NCFM180325). Paratypes. Same data as holotype (NCFM180326-NCFM180328).

Diagnosis

Inversidens rentianensis sp. nov. is morphologically distinct from the other two recognized species within the genus by shell shape, beak position and nacre colour (Table 2). Diagnostic characteristics: shell irregularly subtriangular; curvature of the ventral margin slight, nearly straight; umbo situated 1/2 of shell length; nacre reddish.

Table 2.
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Conchological characters of Inversidens rentianensis sp. nov., Inversidens brandtii, Inversidens pantoensis. Characteristic descriptions of I. brandtii and I. pantoensis are referenced from Kondo (1982, 2008) and He and Zhuang (2013).

I. rentianensis sp. nov. I. brandtii I. pantoensis
Shell shape Irregularly subtriangular Ovate Inequilateral, quadrate
Umbo position 1/2 of shell length 1/4 of shell length 1/3 of shell length
Umbo sculpture Feebly wavy wrinkles Rippled Angularly wrinkled
Surface sculpture Concentric ridges Concentric ridges Irregular growth lines
Nacre colour Reddish Milk-white Bluish
Posterior slope Sharp Blunt Blunt
Ventral margin Nearly straight Arc-shaped Long and straight

Description

Shell irregularly subtriangular, medium thickness, and quite inflated. Anterior margin regularly rounded; ventral margin nearly straight; posterior margin obliquely arc-shaped. Umbo prominent and slightly eroded. Umbo sculptured with feebly wavy wrinkles. Posterior slope formed by the ventral margin and posterior margin low, triangular. Epidermis shining black or with brownish-yellow hue. Only one cardinal tooth in each valve, shape triangular. Laterals thick, a little curved, 2 in each valve. Nacre reddish-bronze in colour.

Length 43–52 mm, height 29–36 mm.

Etymology

The specific epithet is derived from the type locality, Rentian Town.

Distribution

The species is known only from Mianshui River, Rentian Town, Ganzhou City, Jiangxi Province, China (present study) (Fig. 2).

Figure 2. 

Photograph of sampling site of Inversidens rentianensis sp. nov. in China.

GenBank accession number

Holotype, NCFM180325: MZ073336; paratypes, NCFM180326: MZ073337.

Molecular analyses

Pairwise COI sequence divergences from Inversidens brandtii and Inversidens rentianensis sp. nov. were conducted using MEGA 5.0. Based on the uncorrected p-distance model, the intraspecific divergences of I. brandtii and I. rentianensis sp. nov. were both 0.00%. The interspecific divergence of I. brandtii and I. rentianensis sp. nov. was 10.1%. Both BI and ML trees obtained a completely consistent topology. Consistent topology relationships are shown in Figure 3. In the phylogenetic trees, I. rentianensis sp. nov. formed a well-supported sister-group relationship with Inversidens brandtii (PP = 1.00, BS = 100; Fig. 3). The genera Pronodularia and Inversiunio belong to different clades well-separated from Inversidens (Fig. 3).

Figure 3. 

Phylogenetic tree of freshwater mussels inferred from Bayesian Inference (BI) and Maximum Likelihood (ML) analyses of COI barcode. Support values above the branches are posterior probabilities (PP)/bootstrap support (BS). Red font indicates the new species from this study.

Remarks

Species delineation can be problematic in the presence of morphological ambiguities due to phenotypic plasticity and convergence (e.g., cryptic species), especially in mollusks (Zieritz et al. 2010; Inoue et al. 2013). The use of molecular genetics can aid species delineation in the case of phenotypic plasticity and/or convergence (Pieri et al. 2018; Wu et al. 2018). Inversidens rentianensis sp. nov. can be distinguished from congeneric species based on diagnostic characteristics of the shell. In this study, we also analyzed the interspecific divergence between Inversidens brandtii and Inversidens rentianensis sp. nov. based on the COI barcode. The results showed that the average interspecific divergence between the two species was 10.1%, which was much higher than intraspecific divergences. Genetic analysis conducted in this study supports I. rentianensis sp. nov. as a valid species, which can be easily distinguished by the COI barcode.

Acknowledgments

We thank the reviewer Dr Manuel Lopes-Lima, the other anonymous reviewer and the Subject Editor Dr Graham Oliver for valuable comments that have greatly improved this manuscript. This work was supported by the National Natural Science Foundation of China under Grant No.31772412.

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