A taxonomy review of Oreoderus Burmeister, 1842 from China with a geometric morphometric evaluation (Coleoptera, Scarabaeidae, Valgini)

Li Sha; Enrico Ricchiardi; Ming Bai; Xing-Ke Yang

doi:10.3897/zookeys.552.6096

Abstract

The species of the genus Oreoderus are morphologically similar, and can be challenging to distinguish without dissecting the male genitalia. In this study, the Oreoderus species from China are reviewed. Three new species of Oreoderus are described: O. dasystibialis Li & Yang, sp. n., O. brevitarsus Li & Yang, sp. n. and O. oblongus Li & Yang, sp. n. A key of the male Oreoderus and a distribution map are provided. Oreoderus coomani Paulian, 1961 was found as a new record in China. The first description of the female of O. arrowi Ricchiardi, 2001 is provided. Oreoderus humeralis Gestro, 1891, O. quadricarinatus Arrow, 1944, O. crassipes Arrow, 1944, and O. momeitensis Arrow, 1910 are excluded from the Chinese fauna. Furthermore, we utilize geometric morphometric approaches (GM) to analyze the shape variation of four characters (pronotum, elytra, protibia and aedeagus) in Oreoderus. The morphological variations of Oreoderus and the taxonomic value of each character are discussed. The combined analysis of geometric morphometrics and comparative morphology support recognition of the three new species.

Keywords

Beetles, Cetoniinae , China, new species, GM

Introduction

Oreoderus Burmeister, 1842 is a genus of Valgini (Coleoptera: Scarabaeidae) which comprises 29 species, distributed only in the Oriental Region. Adults are flower-visitors, the larvae develop in the rotten wood (with or without termites) (Krikken 1984). This genus can be separated from most of the other Valgini (5-7 teeth on the protibia) by two character states: two or three external teeth on the protibia and the relatively shorter first joint of the hind tarsus compared to the second one. In contrast, the external morphology of Oreoderus is very subtly differentiated among species. For the majority of species, the aedeagus has been the only reliable character widely used in species identification. Some minor differences are observed in external characters (e.g., protibia, pronotum, elytra, etc.) as reported by Ricchiardi (2001), but these characters have not been systematically studied. Furthermore, many of these characters vary in their shape, which is not easily described and compared by traditional morphological approaches.

Geometric morphometrics (GM) is a useful tool for shape analysis in biology. This tool has an important advantage: not only does it offer precise and accurate description, but it also serves the equally important purposes of visualization, interpretation and communication of results (Zelditch et al. 2004, Bai and Yang 2014). With the help of GM, the minor morphological variation of characters (e.g., protibia, pronotum, elytra) can be statistically and scientifically defined and compared. In this paper, the Oreoderus species from China are reviewed. Furthermore, four characters (protibia, pronotum, elytra, and aedeagus) are selected to investigate the morphological variation of Oreoderus based on GM approach and the taxonomic values of these characters are discussed.

Material and methods

Materials

In this study, all known species and three new species described in this paper (32 species and 82 specimens total) of Oreoderus and 2 species (2 specimens) of the out groups Hybovalgus Kolbe, 1904 and Dasyvalgus Kolbe, 1904 were selected for geometric morphometric analyses (Table 1). We selected Hybovalgus and Dasyvalgus as out groups because they are close to Oreoderus and members of subtribe Valgina according to Krikken’s classification (1984). Most images were taken by the authors, except of O. arrowi and O. waterhousei, which were provided by Roberto Poggi (MCSN). Others are from the original references.

Table 1.

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The materials used in the geometric morphometric analyses.

	Species	Characters
	Species	Pronotum	Elytra	Protibia	Aedeagus (♂)
1.	Oreoderus aciculatus Paulian, 1961	1	1	1	1
2.	Oreoderus ahrensi Ricchiardi, 2001	1	1	1	1
3.	Oreoderus argillaceus (Hope, 1841)	1	1	1	1
4.	Oreoderus arrowi Ricchiardi, 2001	6	6	6	5
5.	Oreoderus bengalensis Ricchiardi, 2001	1	1	1	1
6.	Oreoderus bhutanus Arrow, 1910	1	1	1	1
7.	Oreoderus bidentatus Ricchiardi, 2001	2	2	2	1
8.	Oreoderus birmanus Ricchiardi, 2001	1	1	1	1
9.	Oreoderus brevicarinatus (Pic, 1928)	2	1	1	1
10.	Oreoderus brevipennis Gestro, 1891	1	1	1	1
11.	Oreoderus brevitarsus sp. n.	10	10	7	6
12.	Oreoderus clypealis Arrow, 1944	1	1	1	1
13.	Oreoderus coomani Paulian, 1961	9	9	9	7
14.	Oreoderus crassipes Arrow, 1944	1	1	1	1
15.	Oreoderus dasystibialis sp. n.	3	3	3	3
16.	Oreoderus gestroi Ricchiardi, 2001	1	1	1	1
17.	Oreoderus gracilicollis Paulian, 1961	1	1	1	1
18.	Oreoderus gravis Arrow, 1910	1	1	1	1
19.	Oreoderus humeralis Gestro, 1891	1	1	1	1
20.	Oreoderus insularis Ricchiardi, 2001	1	1	1	1
21.	Oreoderus longicarinatus Ricchiardi, 2001	1	1	1	1
22.	Oreoderus maculipennis Gestro, 1891	15	15	11	4
23.	Oreoderus meridionalis Paulian, 1961	1	1	1	1
24.	Oreoderus momeitensis Arrow, 1910	1	1	1	1
25.	Oreoderus oblongus sp. n.	10	10	7	6
26.	Oreoderus pseudohumeralis Ricchiardi, 2001	1	1	1	1
27.	Oreoderus quadricarinatus Arrow, 1944	1	1	2	1
28.	Oreoderus quadrimaculatus Miyake, Yamaguchi & Aoki, 2004	-	-	1	1
29.	Oreoderus rufulus Gestro, 1891	1	1	1	1
30.	Oreoderus siamensis Ricchiardi, 2001	1	1	1	1
31.	Oreoderus sikkimensis Ricchiardi, 2001	1	1	1	1
32.	Oreoderus waterhousei Gestro, 1891	1	1	1	1
33.	Dasyvalgus ichangcius Moser, 1915	1	1	1	1
34.	Hybovalgus yunnanus Moser, 1906	1	1	1	1

The specimens are deposited in the following collections:

ERC Enrico Ricchiardi Collection, Turin, Italy;

IEZU Institute of Applied Entomology, Zhejiang University, Hangzhou, Zhejiang, China;

IZASInstitute of Zoology, Chinese Academy of Sciences, Beijing, China;

MCSNMuseo Civico di Storia Naturale “Giacomo Doria”, Genoa, Italy;

MNHNMuséum national d’Histoire naturelle, Paris, France;

NHML The Natural History Museum, London, United Kingdom;

PCRD Private Collection of Ran DAI, Kunming, Yunnan, China.

Taxonomic approaches

The description of morphological characters follows the terminology of Krikken (1984) and Ricchiardi (2001). Specimen length was measured from the anterior margin of the pronotum to the apex of the pygidium. Specimen width represents the maximum width of the elytra. Type specimens of the new species are deposited in the Institute of Zoology, Chinese Academy of Sciences, Beijing, China (IZAS) and private collection of Enrico Ricchiardi, Turin, Italy (ERC). The images of female genitalia were drawn using Adobe Illustrator CS5, others were taken using a Nikon D5100 digital camera fitted to a Zeiss Stemi 2000-C stereomicroscope and processed in Helicon Focus 5.1 software and Adobe Photoshop CS5. The distribution map was made in ArcGis 10.0.

Character selection

Four characters (pronotum, protibia, elytra, and aedeagus) were examined and analyzed. There was negligible difference in the shape of pronotum and elytra for the male and female. However, sexual dimorphism often occurs in the protibia of cetoniines and other scarabs (Ricchiardi and Perissinotto 2014, McQuate and Jameson 2011, Holm 1993). Differences between both sexes include the number and acuteness of external teeth. Taking this into account, only male specimens were examined for protibia variation.

Geometric morphometric approaches

The morphology of the four characters (pronotum, protibia, elytra, and aedeagus) was represented by curves. Each curve was based on homologous or corresponding criteria. The pronotum was represented by 5 curves. Curve 1 represented the outline of the pronotum, which resampled into 50 semi-landmarks (SLM). Curve 2 and Curve 3 represented the outline of the carinae, which resampled into 15 SLM. Curve 4 and Curve 5 represented the outline of lateral carinae, which resampled into 10 SLM. The pronotum, elytra and aedeagus were each represented by a single curve, which resampled into 50 SLM (Fig. 1A–D).

Figure 1.

Curve selection of four characters. A the red curve (Curve 1) is the outline of pronotum, which resampled into 50 semi-landmarks (SLM); the two green curves (Curve 2, 3) are outline of the carinae, which resampled into 15 SLM; two blue curves (Curve 4, 5) are the outline of lateral carinae, which resampled into 10 SLMB the curve is the outline of elytra, which resampled into 50 SLMC the curve is the outline of protibia, which resampled into 50 SLMD the curve is the outline of the left paramere, which resampled into 50 SLM.

These curves were digitized with tps-DIG 2.05 (Rohlf 2006) and all semi-landmarks were converted to landmarks. Landmark configurations were scaled, translated and rotated against the consensus configuration using the GLS Procrustes superimposition method (Bookstein 1991). The principal component analysis (PCA) and canonical variate analysis (CVA) were analyzed in MorphoJ 1.06c (Klingenberg 2011). Because shape differences among species were studied in the PCA analysis, the average or consensus configuration of landmarks for each species was computed. Minimum spanning trees (MST), based on Euclidean distance of the original data points, was computed in PAST 2.04 (Hammer et al. 2001).

The Procrustes distances and Mahalanobis distances computed from canonical variate analysis (CVA) can be used to explain the differences and modes of evolution. Procrustes distance is a measure of the absolute magnitude of the shape deviation and indicates how big the differences are between the average group shape. Mahalanobis distance provides an indication of how different an individual is relative to the others in the sample, and how distinctly groups are separated from one another.

Results

Taxonomy

Genus Oreoderus Burmeister, 1842

Oreoderus Burmeister, 1842: 726.

Type species

Valgus argillaceus Hope, 1841, by monotypy.

Diagnosis

Oreoderus can be distinguished from all other genera within the tribe Valgini by the following characters: a) protibia with only two or three external teeth; b) the first joint of the hind tarsus shorter than the second one; c) pronotum elongated with four carinae; d) visible sternite V twice longer than sternite IV.

Distribution

China, India, Sikkim, Bhutan, Myanmar, Vietnam, Laos, Thailand, Cambodia, Sri Lanka, Malaysia (Fig. 2).

Figure 2.

Distribution Map. A Distribution of Oreoderus. B Enlargement of Yunnan Province.

Key to the species of the male Oreoderus

1	Pronotal carinae forms two noticeable hooked tubercles at the anterior margin	2
–	Pronotal carinae do not forms any tubercles at the anterior margin	3
2	Pronotal scale tufts not present; propygidial spiracles completely obsolete; propygidium without any scale tufts at the hind margin	O. argillaceus (Hope, 1841)
–	Pronotum with scale tufts on the small lateral carinae; propygidial spiracles moderately elevated; propygidium with two scale tufts at the hind margin	O. insularis Ricchiardi, 2001
3	Pronotal carinae very long, reaching over 3/4 of the pronotum length	4
–	Pronotal carinae not reaching over 2/3 of the pronotum length	5
4	Pronotal carinae obsolete	O. gravis Arrow, 1910
–	Pronotal carinae interrupted twice, sharp	O. longicarinatus Ricchiardi, 2001
5	Pronotal carinae long, reaching about 2/3 of the pronotum length	6
–	Pronotal carinae very short, not reaching 1/2 of the pronotum length	27
6	Pronotal carinae interrupted once, sharp	7
–	Pronotal carinae never interrupted	9
7	Pronotal scale tufts on the small lateral carinae	8
–	Pronotal scale tufts close to the scutellum	O. brevipennis Gestro, 1891
8	Propygidial spiracles moderately elevated; propygidium without any scale tufts at the hind margin	O. meridionalis Paulian, 1961
–	Propygidial spiracles completely obsolete; propygidium with two scale tufts at the hind margin	O. siamensis Ricchiardi, 2001
9	Pronotal carinae obsolete	10
–	Pronotal carinae sharp	11
10	Elytra with four patches of dark-colored scales	O. quadrimaculatus Miyake, Yamaguchi & Aoki, 2004
–	Elytra without patches of scales	O. waterhousei Gestro, 1891
11	Pronotal lateral carinae join the lateral margin or arrive very close	12
–	Pronotal lateral carinae stop well before the lateral margin	14
12	Propygidial spiracles completely obsolete	13
–	Propygidial spiracles sharply elevated	O. momeitensis Arrow, 1910
13	Propygidium without any scale tufts at the hind margin	**O. quadricarinatus Arrow, 1944 and O. rufulus Gestro, 1891^***
–	Propygidium with two scale tufts at the hind margin	O. arrowi Ricchiardi, 2001
14	Third teeth of protibia not present	15
–	Third teeth of protibia present	18
15	Mesotibia without bush of thick scales	16
–	Mesotibia with bush of thick scales	17
16	Meso- and metatibia without a spine at the middle of posterior margin	O. brevitarsus Li & Yang, sp. n.
–	Meso- and metatibia with a spine at the middle of posterior margin	O. ahrensi Ricchiardi, 2001
17	Protibia sharp, cariane reaching over the middle of the pronotum	O. bidentatus Ricchiardi, 2001
–	Protibia blunt, cariane not reaching the middle of the pronotum	O. dasystibialis Li & Yang, sp. n.
18	Third tooth of protibia much smaller than first and second	19
–	Third tooth of protibia similar in size as the second	O. gracilicollis Paulian, 1961
19	Anterior margin of clypeus sinuated, with a bifid processes	O. gestroi Ricchiardi, 2001
–	Anterior margin of clypeus pointed, simply sinuated or rounded but without a bifid process	20
20	Hind margin of propygidium centrally projected toward the back, not pointed	O. humeralis Gestro, 1891
–	Hind margin of propygidium centrally rounded or smoothly curved inward	21
21	First joint of hind tarsi shorter than the second	22
–	First joint of hind tarsi as long as the second	25
22	Propygidium covered by simple, not coffee grain shaped scales	23
–	Propygidium centrally or near the scale tufts with areas of raised c-shaped scales	O. pseudohumeralis Ricchiardi, 2001
23	Propygidial spiracles moderately elevated	O. aciculatus Paulian, 1961
–	Propygidial spiracles completely obsolete	24
24	Meso- and metatibia with a spine at the middle of posterior margin	O. maculipennis Gestro, 1891
–	Meso- and metatibia without a spine at the middle of posterior margin	O. oblongus Li & Yang, sp. n.
25	Pronotal carinae continue after the middle of the length with a triangular area made by C- shaped, black, raised scales that reaches the hind margin; propygidium centrally or near the scale tufts with areas made by raised c-shaped scales; propygidium with two scale tufts at the hind margin	O. bengalensis Ricchiardi, 2001
–	No triangular black scales area is present at the end of the pronotal carinae; propygidium covered by simple, not coffee grain shaped, scales; propygidium without any scale tufts at the hind margin	26
26	Metatibial intrusion interposed between the two mobile spurs present; pronotal carinae not parallel, but arched or sinuated	O. birmanus Ricchiardi, 2001
–	Metatibial intrusion interposed between the two mobile spurs not present; pronotal carinae almost parallel and the included area is narrow	O. coomani Paulian, 1961
27	Pronotal carinae obsolete	28
–	Pronotal carinae sharp	30
28	First joint of hind tarsi shorter than the second	29
–	First joint of hind tarsi as long as the second	O. crassipes Arrow, 1944
29	Propygidium without any scale tufts at the hind margin	O. sikkimensis Ricchiardi, 2001
–	Propygidium with two scale tufts at the hind margin	O. bhutanus Arrow, 1910
30	Third tooth of protibia much smaller than first and second; meso- and metatibia without any scales brush covering the median posterior tooth; first hind tarsi joint shorter than the second	O. clypealis Arrow, 1944
–	Third and second teeth of protibia similar in size; meso- and metatibia with a well noticeable brush made by ochraceous scales covering the medial posterior tooth; first hind tarsi joint as long as the second	O. brevicarinatus (Pic, 1928)

*To separate them see the shape of the parameres.

Review of Oreoderus from China

The genus Oreoderus was recently revised by Ricchiardi (2001), though the Chinese Oreoderus species received little attention. Ma (1993, 1995) recorded four Oreoderusspecies from China: O. humeralis Gestro, 1891, O. quadricarinatus Arrow, 1944, O. crassipes Arrow, 1944, O. momeitensis Arrow, 1910. Unfortunately, Ma misidentified these four species. Based on our examination of specimens deposited in IZAS, we identified O. humeralis Gestro, 1891 sensu Ma (1993) as O. maculipennis Gestro, 1891; O. quadricarinatus Arrow, 1944 sensu Ma (1995) as O. arrowi Ricchiardi, 2001; and O. crassipes Arrow, 1944 sensu Ma (1995) as the new species O. oblongus Li & Yang, sp. n. Moreover, O. momeitensis Arrow, 1910 sensu Ma (1995) is determined to be a new record of O. coomani Paulian, 1961 for China. As a result, O. humeralis Gestro, 1891, O. quadricarinatus Arrow, 1944, O. crassipes Arrow, 1944, and O. momeitensis Arrow, 1910 are to be excluded from the Chinese fauna.

Seven species are now known from China, including the three new species (O. brevitasus Li & Yang, sp. n., O. dasystibialis Li & Yang, sp. n., and O. oblongus Li & Yang, sp. n.), plus a range extension (O. coomani). The three species previously known from China and confirmed in our study are O. arrowi, O. bidentatus, and O. maculipennis.

Oreoderus brevitarsus Li & Yang, sp. n.

http://zoobank.org/6A8CE269-C798-4B12-9CF8-90F3DFD6092E

Fig. 3A–F

Type material examined

Holotype ♂, P.R. CHINA, Zhejiang, Mt. Gutianshan, G45–G15, 560m, broad-leaved mixed forest, 2009.VIII.2–5, leg. Liu Chongling. Holotype deposited in IZAS. Paratypes: P.R. CHINA, 1♂, Zhejiang, Mt. Gutianshan, G24ha-140, 446–715m, Broad-leaved mixed forest, 2009.VII. 5–8, leg. Liu Chongling, (IZAS); 1♀, Zhejiang, Mt. Gutianshan, G24ha-157, 446–715m, broad-leaved mixed forest, 2009.IX. 24–27, leg. Liu Chongling, (IZAS); 1♀, Zhejiang, Mt. Gutianshan, G24ha-83, 446–715m, broad-leaved mixed forest, 2009.VII. 26–29, leg. Liu Chongling, (IZAS); 1♀, Zhejiang, Mt. Gutianshan, G24ha-111, 446–715m, Broad-leaved mixed forest, 2009.VII. 5–8, leg. Liu Chongling, (IZAS); 1♂, Zhejiang, Mt. Gutianshan, 1992.VII.27, leg. Wu Hong, IOZ(E)902215, (IZAS); 1♂, Fujian, Zhang???hu (unrecognized name), 1981.VI.19, IOZ(E)902216, (IZAS); 1♂, Yunnan, Qiubei, Shupi 1278, Quercus, 1500m, leg. Kui Meihua, 1979.VII.6, IOZ(E)902199, (IZAS); 1♀, Yunnan, Xishuangbanna, Mengzhe, 1200m, 1958.VIII.23, leg. Pu Fuji, IOZ(E)902201, (IZAS); 1♂, Yunnan, Xishuangbanna, Xiaomengyang, 850m, 1958.IX.4, leg. MengXuwu, IOZ(E)90221, (IZAS); 1♂, Yunnan, Yiwu, Banna, Menglun, 650m, 1959.VIII.3, leg. Pu Fuji, IOZ(E)902211, (ERC).

Additional material examined

1♀, P.R. CHINA, Zhejiang, Thousand island Lake (IEZU).

Diagnosis

Based on the morphological comparison and PCA analysis of four characters (see below), this new species is close to O. dasystibialis and O. bidentatus, but differs from O. bidentatus by the shape of the carinae of the pronotum and can be separated from O. dasystibialis by the sharp teeth on the protibia and the absence of a thick brush on meso- and metatibia in the male. Finally, the aedeagi are very distinctive in the three species. The parameres are much slender than the other two species and the outer margin is sinuate. The female O. brevitarsus can be distinguished from O. bidentatus by the short stylus and the presence of a brush on the inner margin of the protibia.

Description of the holotype, male

Length 8.2 mm; width 3.9 mm. Color: light brown to brown. Head: clypeus anteriorly rounded, lateral margin extended, with erected setae. Frons densely covered with testaceous scales. Ocular canthus short and broad, covered with same scales as clypeus. Antenna with 10 segments, clubs much longer than antennomeres 2–7. Pronotum: widest at the base, lateral margins sinuate. Surface covered with oblong lied-down scales. Carinae sharp, highly prominent, ending over 1/2 of the pronotum length; lateral small carinae short, sharp, not reaching the lateral margin of the pronotum. Scutellum: triangular, rounded at the apex, covered with shorter oval scales than pronotum. Elytra: with rows of punctate lines, covered with similar scales as scutellum. Propygidium: anteriorly glabrous and slightly punctate, posterior densely punctate and covered with scales. Spiracles moderately elevated. Pygidium: with thick, lied-down scales and one central scale tuft at the hind margin. Venter: coarsely and densely punctate. Visible sternite V smooth and bald in the middle. Visible sternite V twice longer than sternite IV. Legs: slender, femora and tibia covered with testaceous scales except for protibia. Outer margin of mesometatibia covered with more dense scales. Protibia short and bidentate, external teeth sharp. Tarsomeres with short setae. Parameres: short, lateral margin sinuate, the apex covered with yellow setae.

Description of female

Length 7.5–10.4 mm; width 3.5–4.7. Pronotum broader than the male one; carinae much shorter, reaching about 1/3 of the disc. Propygidium much longer, and pygidium with a sharp stylus. Visible sternite V much broader. Protibia short and with thick brush in the inner margin; tooth slightly blunt, mesometatibia with same brush on the inner and outer margin.

Variability

Male paratypes: length 9.0–9.5 mm; width 4.7–5.1 mm, and very similar to the holotype.

Etymology

The new species is named for the short tarsi of protibia.

Distribution

China: Zhejiang, Fujian, Yunnan.

Figure 3.

Habitus of Oreoderus brevitarsus (holotype). A dorsal view B pygidium C aedeagus. Habitus of Oreoderus brevitarsus (female). D dorsal view E pygidium F female genitalia. Scale bars: 1.0 mm.

Oreoderus dasystibialis Li & Yang, sp. n.

http://zoobank.org/EC995BA6-62C2-41A2-9BFA-64C94A25478D

Fig. 4A–C

Type material examined

Holotype, ♂, P.R. CHINA, Hainan, Mt. Jiangfengling, Heiling, 1982.VII.10, leg. Hua Lizhong, Sun Yat-sen University/100/IOZ(E)1658787. Holotype deposited in IZAS. Paratypes: P.R. CHINA: 1♂, Hainan, Mt. Jiangfengling, Wufenqu, 1981.VI.29, leg. Wang (26), Sun Yat-sen University/99/IOZ(E)1658786, (IZAS); 1♂, Hainan, Kwangtung, 1934.IX.1, leg. He Chi, Fan Inst. Biol Peiping/ IOZ(E)902260, (ERC).

Diagnosis

based on the morphological comparison and PCA analysis of four characters (see below), this new species is close to O. brevitarsus and O. bidentatus, but differs from O. bidentatus by the smaller lateral carinae of the pronotum and can be separated from O. brevitarsus by the blunt teeth on protibia and the appearance of a thick brush on meso- and metatibia in the male. The aedeagi are very distinctive among these three species. The apex of the parameres in the new species is much wider than in the other two.

Description of the holotype, male

length 8.5 mm; Width 4.3 mm. Color: light brown to brown. Head: clypeus short, anteriorly straight, with erected setae. Frons densely punctate, covered with testaceous scales. Ocular canthus short, covered with same scales. Antenna with 10 segments, clubs much longer than antennomeres 2–7. Pronotum: widest at the base, lateral margins sinuate. Surface densely punctate, covered with thick testaceous scales. Carinae sharp, highly prominent, ending at 2/3 of the pronotum length; lateral small carinae long, sharp, reaching the lateral margins of the pronotum. Scutellum: triangular, rounded at the apex, covered with testaceous scales. Elytra: with rows of punctate line, covered with same scales as scutellum. Propygidium: covered with lied down scales. Spiracles moderately elevated. Pygidium: triangular form, coarsely punctate, covered with thick lied down oval scales. Venter: coarsely and densely punctate with each point bearing a testaceous scale. A longitudinal groove is visible in the middle of visible sternites I–IV; visible sternite V longer twice than the sternite IV. Legs: slender, covered with testaceous scales except protibia. Protibia extended, bidentate, teeth blunt. Meso- and metatibia with thick brush on the outer margins. Tarsomeres with short setae. Parameres: relatively longer and much broader, the apex is the widest part.

Female

unknown.

Variability

paratypes length 7.5–7.7 mm; width 3.8–4.2 mm, and very similar to the holotype.

Etymology

the new species is named according to its thick brush on meso- and metatibia.

Distribution

China: Hainan.

Remarks

only three males were collected in Hainan Island, two of them on Mt. Jianfengling.

Figure 4.

Habitus of Oreoderus dasystibialis (holotype). A dorsal view B pygidium C aedeagus. Scale bars: 1.0 mm.

Oreoderus oblongus Li & Yang, sp. n.

http://zoobank.org/6CDA60F6-63CC-4720-80A6-57AE6B9F04E9

Fig. 5A–F

Type material examined

Holotype, ♂, P.R. CHINA, Yunnan, Xishuangbanna, Jinghong, 650m, 1958.VIII.12, leg. Meng Xuwu, IOZ(E)902189. Holotype deposited in IZAS. Paratypes: P.R. CHINA: 1♂, Yunnan, Xishuangbanna, Xiaomengyang, 850m, 1958.VIII.19, leg. Zhang Yiran, IOZ(E)902182, (IZAS); 1♂, Yunnan, Xishuangbanna, Xiaomengyang, 850m, 1958.VIII.20, leg. Pu Fuji, IOZ(E)902183, (IZAS); 1♂, Yunnan, Xishuangbanna, Xiaomengyang, 850m, 1958.IX.2, leg. Zheng Leyi, IOZ(E)902185, (IZAS); 1♂, Yunnan, Xishuangbanna, Jinghong, 650m, 1958.VIII.26, leg. Meng Xuwu, IOZ(E)902186, (IZAS); 1♂, Yunnan, Xishuangbanna, Jinghong, 650m, 1958.VII.15, leg. Meng Xuwu, IOZ(E)902188, (IZAS); 1♂, Yunnan, Xishuangbanna, Menglun, 580m, 1993.IX.10, leg. Xu Huanli, IOZ(E)902204, (IZAS); 1♂, Yunnan, Xishuangbanna, Menglun, 600m, 1993.IX.9, leg. Yang Longlong, IOZ(E)902205, (IZAS); 1♂, Yunnan, Xishuangbanna, Jinghong, 650m, 1958.VII.27, leg. Meng Xuwu, IOZ(E)902208, (ERC); 1♀, Yunnan, Xishuangbanna, Xiaomengyang, 850m, 1957.X.20, leg. Pu Fuji, IOZ(E)902223, (IZAS).

Diagnosis

Based on the morphological comparison and PCA analysis of four characters (see below), this new species is close to O. maculipennis, but lacks a spine on the outer margin of meso- and metatibia and elytra without white patch scales in the new species. The parameres are very short in O. oblongus.

Description of the holotype, male

Length 8.4 mm; width 4.0 mm. Color: light brown to brown. Head: clypeus anteriorly rounded, with erect setae on the anterior margin. Frons covered with testaceous scales. Ocular canthus short, covered with same scales. Antenna with 10 segments, clubs not much longer than antennomeres 2–7. Pronotum: widest at base, lateral margins sinuate. Surface densely punctate, covered with lied down scales. Carinae sharp, highly prominent, ending around 2/3 of the pronotum length; Lateral small carinae short, sharp, not joining the lateral margin of the pronotum. Scutellum: triangular, rounded at the apex, covered with testaceous scales. Elytra: with rows of punctate line, densely covered with oval scales. Propygidium: broad, punctate, covered with lied down scales. Spiracles moderately elevated. Pygidium: triangular, punctuation rounded, with thick lied down scales. Venter: coarsely and densely punctate with testaceous scales. Visible sternite V twice longer than sternite IV. Legs: slender, covered with oval scales except for protibia. Protibia tridentate; the third tooth small, far from the first two teeth. Meso- and metatibia covered with only sparse scales. Tarsomeres with short setae. Parameres: perpendicular to phallobase, the apex is sharp.

Description of female

Length 9.7 mm; width 4.5 mm. Pronotum a little broader than the male; carinae slightly shorter. Propygidium much longer, and pygidium more highly prominent. Visible sternite V much broader. External tooth of protibia apparently blunt. Tarsomeres more robust.

Variability

paratypes length 7.0–9.2 mm; width 3.7–4.3 mm, and very similar to the holotype.

Etymology

the new species is named for the oblong shape of the body.

Distribution

China: Yunnan.

Figure 5.

Habitus of Oreoderus oblongus (holotype). A dorsal view B pygidium C aedeagus. Habitus of Oreoderus oblongus (female). D dorsal view E pygidium F female genitalia. Scale bars: 1.0 mm.

Oreoderus coomani Paulian, 1961

Oreoderus coomani Paulian, 1961: 31.

Type material examined

Lectotype (designated by Ricchiardi, 2001), ♂, Tonkin, Hoa-Binh, A. de Cooman, (MNHN).

Additional material examined

P.R. CHINA: 1♂, Yunnan, Xihuangbanna, Xiaomengyang, 850m, 1957.X.20, leg. ZangLingchao, IOZ(E)902180; 1♂, Yunnan, Xihuangbanna, Menglun, 600m, 1993.IX.11, Mt. Shihuishan, leg. Yang Longlong, IOZ(E)902190; 1♂, Yunnan, Xihuangbanna, Menghai, 1100m, 1957.VIII.15, leg. Wang Shuyong, IOZ(E)902191; 1♂, Yunnan, Xihuangbanna, Menga, 1050–1080m, 1958.VIII.12, leg. Wang Shuyong, IOZ(E)902192; 1♂, Yunnan, Xihuangbanna, Menga, 1050–1080m, 1958.VIII.7, leg. Pu Fuji, IOZ(E)902193; 1♂, Yunnan, Xihuangbanna, Menga, 1050–1080m, 1958.VIII.20, leg. Pu Fuji, IOZ(E)902194; 1♂, Yunnan, Xihuangbanna, Menga, 1050–1080m, 1958.VIII.19, leg. Wang Shuyong, IOZ(E)902195; 1♂, Yunnan, Xihuangbanna, Menga, 1050–1080m, 1958.VIII.10, leg. Wang Shuyong, IOZ(E)902196; 1♂, Yunnan, Xihuangbanna, Xiaomengyang, 850m, 1957.X.26, leg. Wang Shuyong, IOZ(E)902197; 1♂, Yunnan, Malipo, 1958.VII.21, (IZAS).

Distribution

China: Yunnan; Vietnam and Laos.

Remarks

This species was previously known from Vietnam and Laos. This is the first record for Yunnan, China.

Oreoderus arrowi Ricchiardi, 2001

Fig. 6A–C

Oreoderus arrowi Ricchiardi, 2001: 521.

Type material examined

Holotype, ♂, S. China, 10–14.VII.1990, Jinghong, Prov., Yunnan, leg. S. Bečvář, (MCSN).

Additional material examined

P.R. CHINA: 1♂, Yunnan, Xihuangbanna, Jinghong, 650m, 1958.VII.7, leg. MengXuwu, IOZ(E)902175; 1♂, Yunnan, Xihuangbanna, Damenglong, 650m, 1958.VII.11, leg. ZhengLeyi, IOZ(E)902176; 1♂, Yunnan, Xihuangbanna, Damenglong, 650m, 1958.VII.11, leg. ZhengLeyi, IOZ(E)902177; 1♂, Yunnan, Xihuangbanna, Mengzhe, 870m, 1958.IX.7, leg. Wang Shuyong, IOZ(E)902178; 1♂, Yunnan, Xihuangbanna, Xiaomengyang, 850m, 1958.IX.2, leg. MengXuwu, IOZ(E)902179; 1♂, Yunnan, Xihuangbanna, Xiaomengyang, 1400m, 1957.X.4, leg. Wang Shuyong, IOZ(E)902187; 1♀, Yunnan, Naban River Nature Reserve, Mengsong, Danuoyou, 2007.XII.14, 770m, Danuoyou IV A, 14.XII.2007, leg. A. Weigel, 22.20699°N, 100.63761°E (trap), leg. A. Weigel, IOZ(E)1945434, (IZAS).

Description of female

Length 8.4 mm; width 2.8mm. Color: light brown to brown. Head: clypeus anteriorly rounded, sharp in the apex, with erected setae. Frons covered with testaceous scales. Ocular canthus short and broad, covered with same scales. Antenna with 10 segments, club much longer than antennomeres 2–7. Pronotum: widest at base, lateral margin sinuate. Surface densely punctate, covered with testaceous scales. Carinae and lateral carinae sharp, highly prominent, ending before middle of pronotum. Scutellum: triangular, rounded at apex, covered with testaceous scales. Elytra: coarsely punctate, covered with testaceous scales. Propygidium: apparently longer than in male, hind margin rounded. Propygidial spiracles moderately elevated. Pygidium: narrower than in male, with thick lied down scales. Venter: coarsely and densely punctate with testaceous scale. Sternite V twice longer than Sternite IV; Sternite VI much narrower than male. Legs: slender, covered with testaceous scales except protibia. Protibia tridentate, tooth blunter than in male; meso- and metatibia with a spine on the outer margin. Tarsomeres much shorter than in male, covered with short setae.

Distribution

China: Yunnan.

Figure 6.

Habitus of Oreoderus arrowi (female). A dorsal view B pygidium C female genitalia. Scale bars: 1.0 mm.

Oreoderus bidentatus Ricchiardi, 2001

Oreoderus bidentatus Ricchiardi, 2001: 526.

Type material examined

Holotype, ♂, India, Meghalaya, Kashia Hills. Paratype, 1♀, Yunnan, Bao Shan, 1700 m, 1993.V.1–3, (NHML).

Additional material examined

1♀, Yunnan, Mt. Gaoligong, (PCRD).

Distribution

China: Yunnan; North East India.

Remarks

Oreoderus bidentatus was described from three males from Assam (2 paratypes) and Meghalaya (holotype) and one female from Yunnan (Ricchiardi 2001). One of the authors (Ricchiardi 2001) decided to determine the female specimen from Yunnan as belonging to this species because its two-teethed protibia and other characters similar to the males of O. bidentatus (shape of pronotum and carinae; anterior margin of clypeus, etc.). The second female specimen from the same Chinese Province was identical to the paratype. We hope that the finding of a male specimen from Yunnan will definitively confirm the distribution of this species. The absence of findings of O. bidentatus in Myanmar is probably only due to the lack of research in that country.

Oreoderus maculipennnis Gestro, 1891

Oreoderus maculipennis Gestro, 1891: 869.

Type material examined

Holotype, ♂, MCSN, Birmania, Bhamo, VI-1885, Leg. Fea.

Additional material examined

1♂, Fujian, Shanghang, 650m, 1988.VII.24, IOZ(E)902212; 1♂, Fujian, Shanghang, 650m, 1988.VII.23, IOZ(E)902213; 1♂, Yunnan, Xishuangbanna, Xiaomengyang, 850m, 1957.X.26, leg. Wang Shuyong, IOZ(E)902203; 1♂, Yunnan, Naban, II/3D, 10.XI.2008, leg. L.Z. Meng, Yunnan, Jinghong, Naban River Nature Reserve Chachang (Forest), 2008.XI.10, 729m, 22.15810°N, 100.66543°E, leg. Meng Lingzeng; 1♀, Yunnan, 991 Fengqing, Fengshan, 1600m, leg. Zhang Fu, 1980.VII.26, IOZ(E)902214; 1♀, Yunnan, Xishuangbanna, Xiaomengyang, 850m, 1957.X.26, leg. Wang Shuyong, IOZ(E)902218; 1♂, Yunnan, Xishuangbanna, Xiaomengyang, 850m, 1957.X.26, leg. Wang Shuyong, IOZ(E)902219; 1♂, Yunnan, Xishuangbanna, Xiaomengyang, 850m, 1957.X.26, leg. Wang Shuyong, IOZ(E)902220; 1♂, Yunnan, Xishuangbanna, Xiaomengyang, 850m, 1957.X.25, leg. Wang Shuyong, IOZ(E)902221; 1♂, Yunnan, Xishuangbanna, Xiaomengyang, 850m, 1957.X.21, leg. Zang Lingchao, IOZ(E)902222; 1♀, Yunnan, Xishuangbanna, Menghai, 1200–1600m, 1958.VII.22, leg. Pu Fuji, IOZ(E)902224; 1♀, Yunnan, Xishuangbanna,, Mengzhe, 1200m, 1958.VIII.28, leg. Wang Shuyong, IOZ(E)902226; 1♂, Naban II/3D, 10.XI.2008, leg. L.Z. Meng, Yunnan, Jinghong, Naban River Nature Reserve Chachang (Forest), 2008.XI.10, 729m, 22.15810°N, 100.66543°E, leg. Meng Lingzeng; 1♂, Naban II/3D, 20.XI.2008, leg. L.Z. Meng, Yunnan, Jinghong, Naban River Nature Reserve Chachang (Forest), 2008.XI.20, 729m, 22.15810°N, 100.66543°E, leg. Meng Lingzeng, (IZAS).

Distribution

China: Yunnan; Myanmar.

Remark

First record of Oreoderus maculipennis from Yunnan Province which confirmed the prediction of Ricchiardi (2001).

Morphological variations of Oreoderus

Oreoderus is the largest genus in the tribe Valgini and easily distinguished by its covering of scales. The clypeus is usually rounded in front except for O. clypealis (the front margin straight and recurved), O. gestroi, O. gravis and O. waterhousei (with a process on the front margin). The pronotum is nearly trapezoidal, longer than wide. There are two pairs of carinae on the pronotum, the length of carinae varies among species. The middle carinae are moderately prominent except O. insularis, O. argillaceus (highly prominent, forming two tubercles) and O. coomani (only slightly prominent). The elytra are short and broad, similar morphologically and sometimes decorated with patches of scales (O. bidentatus, O. maculipennis and O. birmanus etc.). The pygidium is nearly triangular, covered with thick scales. Females of some species have a stylus on the hind margin (O. bidentatus, O. brevitasus).

The morphological variation of four characters (pronotum, protibia, elytra and aedeagus) was investigated based on 34 species (84 specimens) using geometric morphometrics. The shape information was extracted from the landmark data using the Procrustes fit. To see the variations, we used the principal component analysis (PCA). The first two PCs together accounted for 77.11%, 89.14%, 60.50% and 55.96% of the total variance in the analysis of pronotum, elytron, protibia and aedeagus, respectively. The main shape change of the pronotum was observed in the length/width ratio of the pronotum and the carinae (Fig. 7A). The main shape change of the elytra was observed in the length/width ratio (Fig. 7B). The main shape change of the protibia was observed in the length/width ratio, while a secondary shape change can be observed in the curvature of the second teeth on protibia (Fig. 7C). The main shape change of the paramera was in their base, while a secondary shape change can be observed in the length/width ratio of the external part of parameres (Fig. 7D). Morphological variation in the out groups fell within the morphological space of Oreoderus in all four characters. The similarity among species in these four characters is also reflected in the Minimal Spanning Tree (MST) (Suppl. material 1: Fig. A.1).

Figure 7.

Principal component analysis (PCA) based on the shape variations of different characters. The averaged shape of extreme specimens is depicted as deformations using thin plate splines. A pronotum B elytra C protibia D aedeagus.

Based on the PCA results, the morphological diversity in these four characters suggest they are not equally diagnostic in Oreoderus (Table 2). The highest morphological variation and clearest differentiation among species is found in the parameres. The second most diagnostic character is the protibia and the third is the pronotum. The elytron provides the least morphological resolution among Oreoderus species.

Table 2.

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Total variance of four characters.

Characters	Total variance
pronotum	0,015
elytra	0,002
protibia	0,016
aedeagus	0,020

Validity of the new species

To extend the results of the comparative morphological analysis, a canonical variate analysis (CVA) of Chinese Oreoderus including the new species was conducted to quantitatively assess the differences among species, with a particular focus on differentiating the new species. The canonical variates scores of pronotum variables showed the 90% equal frequency ellipse, although there is some overlap between O. maculipennis and O. oblongus (Fig. 8A). The morphological differences based on Mahalanobis distances among the seven species are all highly significant in all pairwise comparisons (p<0.05). Similar results were found for the other morphological variables, as Mahalanobis distances based on the morphology of elytra, protibia and aedeagus were all highly significant in all pairwise comparisons (p<0.05) (Suppl. material 1: Table A.1–4; Fig. 8B–D).

Figure 8.

Canonical variate analysis (CVA) based on the shape variations of different characters showing 90% confidence ellipses of population means. A pronotum B elytra C protibia D aedeagus.

Most obtained p-values from permutation tests (10000 permutation rounds) for Procrustes distances based on the morphology of four characters (pronotum, elytra, protibia and aedeagus, respectively) among the seven species were smaller than 0.05 (Suppl. material 1: Table A.1–4).

Discussion

In this study, the taxonomy values of four characters (pronotum, elytra, protibia and aedeagus) were evaluated in Oreoderus. According the CVA analyses above, the studied specimens were clustered into seven groups for all four morphological characters. Comparisons among species were significantly different when quantified by Mahalanobis distances. This meant the morphological boundary of species based on the sample specimens from the Oreoderus species were distinctly separated from each other. In other words, the Oreoderus species could be statistically separated and determined based on Mahalanobis distances of the four characters. However, the average shape of these four characters was not always significantly different when measured by Procrustes distance, and may not be a useful metric for taxonomic evaluations of Oreoderus. Our results demonstrate that geometric morphometric analysis of external and internal characters can enhance species diagnosis in cryptic species.

The taxonomical value of the four characters examined was not equivalent. The aedeagus of Oreoderus is very distinctive in Valgini, with an overall longer and more robust shape. Also, the aedeagus contains the most morphological variation in Oreoderus, and provides the best character for taxonomic determination in this genus.

The GM analysis suggests that the pronotum and protibia can also be informative in the taxonomy of Oreoderus. The shape of pronotum in Oreoderus is nearly trapezoidal, longer than it is wide. The apical part is usually narrow and the lateral margin is sinuate. The main shape variations are the outline of pronotum, length and relative position of the carinae on the pronotum according to the result of PCA. The protibia of Oreoderus is usually short and flat, dentate with only one spur. The main variation of the protibia is on the external teeth according to the result of PCA.

The numbers of the external teeth of protibia are already used in the taxonomy of Valgini (Paulian 1961, Ricchiardi 1994, Krajčík 2011). For example, the presence of two to three teeth on the outer margin of the protibia is diagnostic of Oreoderus, whereas five or more teeth are found in other genera within Valgini. For other members of the Cetoniinae, the male and female usually differ in the number of protibial teeth and the last teeth are always absent or very small in the females. Compared with the common use of the numbers of teeth, the shape of the protibia is rarely used in the taxonomy of Oreoderus and other Valgini. Our results suggest that the shape of the protibia is diagnostic among the species of Oreoderus.

The elytra of Oreoderus are flat and covered with scales. The elytra contained the least morphological variation among four characters in this study. Additionally, the out groups were not separated from Oreoderus. The taxonomic value of the elytral shape is not highly supported.

Traditionally, discrete characters are commonly used in taxonomy. However it is often difficult to find enough discrete characters to resolve confusing taxonomic problem, such as the morphological convergence of Oreoderus. In such a case, geometric morphometric (GM) can been used (Villemant et al. 2007, Hájek and Fikáček 2010, Xu et al. 2013, Bai et al. 2014, Zúñiga-Reinoso and Benitez 2015). Our study is the first to apply this approach to analyze shape variation in Valgini and demonstrate that this tool can be used to resolve this sort of problem. Based on our results, we suggest that future studies will benefit from by incorporating geometric morphometric techniques, and could, for example, examine unknown species of Oreoderus in combination with our data to investigate the possible status of an unknown specimen. Additionally, other characters, such as continuously variable characters, could be examined in addition to those we studied, in order to help resolve morphological differences in other species.

Acknowledgments

We sincerely thank the museums and institutions that loaned the specimens for our research. Special thanks to Dr Johannes Frisch and Joachim Willers (ZMHB) for loaning specimens; Dr Olaf Jaeger (STMD), Dr Olivier Montreuil and Antoine Mantilleri (MNHN) for providing the type species; Dr Roberto Poggi (MCSN) for providing photos of specimens. Thanks are given to Dr Sean Schoville (University of Wisconsin, Madison, USA) for providing suggestions in writing our manuscript.

This research was supported by the National Basic Research Program of China (973 Program, 2011CB302102); the National Natural Science Foundation of China (31172143, 51305057); and by a Humboldt Fellowship (M.B.) from the Alexander von Humboldt Foundation.

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Supplementary material

Supplementary material 1

Figure A.1 Minimum spanning tree mapped onto a PCA plot; Table A.1–4 Difference in shapes of four characters among species.

Sha Li, Enrico Ricchiardi, Ming Bai, Xingke Yang

Data type: calculation results

This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.

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