Checklist |
Corresponding author: Carlos Daniel Pinacho-Pinacho ( danyboy_jd26@hotmail.com ) Academic editor: David Gibson
© 2015 Carlos Daniel Pinacho-Pinacho, Martín Garcia-Varela, Jesus S. Hernandez-Orts, Carlos A. Mendoza-Palmero, Ana L. Sereno-Uribe, Emilio Martinez-Ramirez, Leopoldo Andrade-Gomez, Alejandra López-Jiménez, Eduardo Hernadez-Cruz, Gerardo Pérez Ponce de León.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Pinacho-Pinacho CD, García-Varela M, Hernández-Orts JS, Mendoza-Palmero CA, Sereno-Uribe AL, Martínez-Ramírez E, Andrade-Gómez L, López-Jiménez A, Hernández-Cruz E, de León GP-P (2015) Checklist of the helminth parasites of the genus Profundulus Hubbs, 1924 (Cyprinodontiformes, Profundulidae), an endemic family of freshwater fishes in Middle-America. ZooKeys 523: 1-30. https://doi.org/10.3897/zookeys.523.6088
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From December 2012 to November 2014, 267 fish belonging to the family Profundulidae (representing nine of the 11 species of the genus Profundulus) were collected in 26 localities of Middle-America, across southern Mexico, Guatemala, and Honduras, comprising the distribution range of the genus, and analyzed for helminth parasites. Additionally, a database with all ten available published accounts of the helminth parasite fauna of this genus (the only genus within the family) was assembled. Based on both sources of information, a checklist containing all the records was compiled as a tool to address future questions in the areas of evolutionary biology, biogeography, ecology and phylogeography of this host-parasite association. The helminth parasite fauna of this fish group consists of 20 nominal species, classified in 17 genera and 14 families. It includes six species of adult digeneans, five metacercariae, two monogeneans, one adult cestode, three adult nematodes and three larval nematodes. The profundulid fishes are parasitized by a specialized group of helminth species (e.g.Paracreptotrema blancoisensu
Killifish, Profundulidae , Middle-America, Digenea , Monogenea , Cestoda , Nematoda
The information gathered regarding the composition of the helminth parasites of freshwater fishes of Mexico has increased in recent years (
The distribution of the Profundulidae extends along the Atlantic and Pacific Ocean slopes of southern Mexico, Guatemala, El Salvador and Honduras (
Records of the helminth parasite fauna of this family began with
As a continuation of effort to provide a more complete inventory of the helminth parasite fauna of freshwater fishes in this geographical region, intensive samplings were conducted during the last few years of these killifishes throughout their ranges of distribution in an attempt to obtain empirical and robust data to enable further studies of this host-parasite association from a phylogenetic, phylogeographical and biogeographical perspective, with the aim of understanding the mechanisms that have shaped the evolutionary and biogeographical history of these fishes and their parasites in Middle-America. The main objectives of this research were (1) to compile all the available published accounts on the helminth fauna of profundulid fishes, and (2) to incorporate new data derived from our own survey work of the last few years.
Data compilation. A dataset of all published records of the helminth species reported from members of the family Profundulidae in Middle-America was compiled. The keywords “Parasit(e)*AND Profundulus” were used to conduct searches through the ISI Web of Science. All those studies whose datasets provided taxonomic information on the helminth taxa found in a sample of individual hosts were considered.
Current research. Original data from our own studies of the last two years were included. From December 2012 through to November 2014, 267 individual fish belonging to nine species of Profundulus, i.e. P. balsanus; P. candalarius Hubbs, 1924; P. guatemalensis (Günther, 1866); P. hildebrandi Miller, 1950; P. kreiseri Matamoros, Schaefer, Hernández & Chakrabarty, 2012; P. labialis; P. portillorum Matamoros & Schaefer, 2010; P. punctatus (Günther, 1866); and Profundulus sp. 2 (sensu
A Map of Middle-America indicating the localities where specimens of Profundulidae have been have been examined for helminth parasites. Colors and symbols correspond for each species of Profundulus B Zoom of an endorrheic basin in San Cristóbal de la Casas, Chiapas, Mexico showing localities where the endemic fish P. hildebrandi was collected.
Localities in Mexico, Guatemala, El Salvador and Honduras where at least one helminth species has been recorded as a parasite of Profundulus. Localities marked with an asterisk (*) were sampled in this study. Collection sites (CS), locality (four letters code), geographical coordinates, country sampled and references are included. The collection site (CS) and locality code correspond with the localities referred in Table
CS | Locality (code) | Geographical coordinates | Country | References | |
---|---|---|---|---|---|
N | W | ||||
(1) | Arroyo Inzcuinatoyac (Inzc) | 17°21'39" | 99°44'00" | México |
|
(2) | Río Suchiapa (Such) | 16°20'06" | 93°27'19" | México |
|
(3) | Ecosur (Ecos) | 16°42'55" | 92°37'28" | México |
|
(4) | La Albarrada (Alba) | 16°42'37" | 92°37'32" | México |
|
(5) | 5 de Marzo (5mar) | 16°42'34" | 92°38'14" | México |
|
(6) | El Puente (Puen) | 16°43'59" | 92°36'54" | México |
|
(7) | Arroyo Chamula (Cham) | 16°44'52" | 92°39'22" | México |
|
(8) | Peje de Oro (Peor) | 16°44'48" | 92°37'00" | México |
|
(9) | El Arcotete (Arco) | 16°45'57" | 92°31'43" | México |
|
(10) | Arenal (Aren) | 16°43'31" | 92°34'53" | México |
|
(11) | Agua de Pajarito (Paja) | 16°43'43" | 92°34'44" | México |
|
(12) | Laguna Soyul (Lsoy) | 16°46'01" | 92°31'39" | México |
|
(13) | Río Ocotlán (Ocot) | - | - | México |
|
(14) | Río Chicomosuelo (Chic) | 15°44'38" | 92°16'50" | México |
|
(15) | Río Suchiapa, José María Garza (Rsuc) | 16°36'36" | 93°05'03" | México |
|
(16) | Río San Juan, puente El Tablón, Villa Flores (Saju) | 16°21'01" | 93°30'56" | México |
|
(17) | Piedra Labrada (Labr) | 18°58'54" | 99°14'12" | México |
|
(18) | Río La Soledad Carrizo (Carr) | 16°25'0.4" | 97°40'12.9" | México |
|
(19) | Río San José de las Flores (Flor) | 16°24'21.5" | 97°44'22.6" | México |
|
(20) | Río Santa Cruz Flores Magón (Fmag)* | 16°21'6.1" | 97°45'38.3" | México |
This study |
(21) | Río Pichuaca (Pich)* | 16°05'34.2" | 97°24'18.1" | México |
This study |
(22) | Río La Reforma (Refo) | 16°08'33.5" | 97°08'41.6" | México |
|
(23) | Río Pueblo Viejo (Viej)* | 16°06'22.3" | 97°03'47.8" | México |
This study |
(24) | Río Santa María Huatulco (Huat)* | 15°50'14.2" | 96°19'30.8" | México |
This study |
(25) | Río Macuta (Macu) | - | - | México |
|
(26) | Río Templo, San Juan del Río (Sjri)* | 16°53'56.3" | 96°09'57.3" | México |
This study |
(27) | Arroyo Ojo de Agua (Ojag) | 16°13'38.6" | 95°49'36.6" | México |
|
(28) | Río La Laca (Rlac) | 17°14'09.3" | 98°39'55.7" | México |
|
(29) | Río Cahoapan (Caho) | 17°16'37.8" | 99°35'04.7" | México |
|
(30) | Río Tamarindo (Tama) | 17°00'36.5" | 99°06'0.8" | México |
|
(31) | Río del Aguacate (Agua) | 16°07'19" | 97°8'22.8" | México |
|
(32) | Arroyo los Sabinos (Sabi) | 16°25'39.9" | 97°4'28.9" | México |
|
(33) | Río Chacalapa (Chac) | 15°55'54.8" | 95°56'00.3" | México |
|
(34) | Río Chicaguaxtla (Chic) | 17°03'30.30" | 97°51'32.52" | México |
|
(35) | Río Chico (Rchi) | 16°55'34.50" | 96°12'27.42" | México |
|
(36) | Amatenango del Río (Amri) | 16°31'22.2" | 92°25'10.7" | México |
|
(37) | Río Grande de Comitán (Rcom) | 16°16'49.9" | 92°07'21.1" | México |
|
(38) | Arroyo ECOSUR (Aeco) | 16°42'27.3" | 92°36'54.8" | México |
|
(39) | Arroyo Teopisca (Ateo) | 16°33'13.7" | 92°28'34.9" | México |
|
(40) | Río Rancho San Antonio (Rsan) | 16°58'30.9" | 93°03'44.7" | México |
|
(41) | Arroyo Tres Picos (Atpi) | 17°03'28.3" | 93°11'50.7" | México |
|
(42) | Río Nandalumi (Rnan) | 16°43'18.4" | 92°58'52.4" | México |
|
(43) | Arroyo Ojo de Agua, El Canelar (Cane) | 16°32'08" | 92°55'02.5" | México |
|
(44) | Río Nil (Rnil) | 14°33'54.4" | 91°43'25.4" | Guatemala |
|
(45) | Río el Cantil (Cant) | 14°21'22.6" | 90°48'30.4" | Guatemala |
|
(46) | Arroyo El Platanar (Apla) | 14°36'58.6" | 90°46'37.9" | Guatemala |
|
(47) | Río Cauca (Rcau) | 13°46'42.6" | 89°51'40.8" | El Salvador |
|
(48) | Río Cauca (Cauc) | 13°46'41.6" | 89°46'41.67" | El Salvador |
|
(49) | Quebrada Los Tecomates (Qtec) | 14°18'11.3" | 89°09'40.8" | El Salvador |
|
(50) | Río Nonoalpa (Nono) | 14°17'23.2" | 89°09'10.7" | El Salvador |
|
(51) | Río Ojojona (Ojoj) | 13°55'43.7" | 87°17'40" | Honduras |
|
(52) | Lepaterique (Lepa) | 14°03'42.9" | 87°27'58.6" | Honduras |
|
(53) | Lepaterique (1Lep) | 14°04'14.4" | 87°25'56.9" | Honduras |
|
(54) | El Platanar, Putla de Guerrero (Plat)* | 16°44´55˝ | 97°59´32˝ | México | This study |
(55) | Río San José, Santiago Jamiltepec (Jami)* | 16°24´19˝ | 97°44´20˝ | México | This study |
(56) | Río San Juan, Cristobal Obregón (Obre)* | 16°21´00˝ | 93°30´54˝ | México | This study |
(57) | Río Suchiapan, La Esperanza (Espe)* | 16°23´27.60˝ | 93°17´24˝ | México | This study |
(58) | Río Pedregal, Tres Picos (Tpic)* | 15°55´1.2˝ | 93°32´45.6˝ | México | This study |
(59) | Río Huixtla (Huix)* | 15°10´18˝ | 92°25´24˝ | México | This study |
(60) | El Triunfo (Triu)* | 15°20´44˝ | 92°32´30˝ | México | This study |
(61) | Río Nueva Francia (Fran)* | 15°22´7.58˝ | 92°35´20.2˝ | México |
This study |
(62) | Río Nahualate (Nahu)* | 14°26´44˝ | 91°22´56˝ | Guatemala | This study |
(63) | Río Primavera (Prim)* | 14°22´19.20˝ | 91°09´60˝ | Guatemala | This study |
(64) | Río Escuintla (Escu)* | 14°19´41.51˝ | 91°42´57.35˝ | Guatemala | This study |
(65) | Río Las Cabezas, Saranate (Sara)* | 14°44´23˝ | 90°04´52˝ | Guatemala | This study |
(66) | Puente Sansare (Sans)* | 14°44´52˝ | 90°06´33˝ | Guatemala | This study |
(67) | Río Hondo (Rhon)* | 15°03´55.50˝ | 89°35´48.28˝ | Guatemala | This study |
(68) | Arroyo en Hidroeléctrica Chamelecón (Cham)* | 15°11´51.60˝ | 89°36´57.60˝ | Honduras | This study |
(69) | Quebrada El Paraiso (Qpar)* | 15°01´26˝ | 88°59´32˝ | Honduras | This study |
(70) | Los Potrerillos (Lpot)* | 14°32´31˝ | 87°52´55˝ | Honduras | This study |
(71) | Río San Carlos (Rcar)* | 16°19´10˝ | 91°58´06˝ | México | This study |
(72) | Río La Gloria (Lglo)* | 16°30´01˝ | 92°26´01˝ | México | This study |
(73) | Arroyo Moxviquil (Moxv)* | 16°54´9.00˝ | 92°37´50˝ | México | This study |
(74) | Arroyo Peje de Oro (Poro)* | 16°44´48˝ | 92°36´60˝ | México | This study |
The data analysis of both the bibliographic information and the survey work shows that 11 species of Profundulus (including undescribed species) studied for helminths, and that the list of helminth parasites of fish of this genus consists of 20 species classified in two taxonomic groups: Platyhelminthes (six adult digeneans, five metacercariae, two monogeneans and one adult cestode) and Nematoda (three adults and three larvae). Interestingly, no acanthocephalans and no larval cestodes are part of the helminth fauna of this fish group across its geographical distribution. Most taxa were identified to species level, except for larval stages which lacked the diagnostic characteristics present only in adult forms which are found in fish-eating birds (or freshwater turtles in the case of Spiroxys sp.). To better visualize the information from the checklist, the results are presented in two tables. Table
Parasite-host list of Profundulus in Middle-America. Locality abbreviations (CS-Code) correspond to those in Table
Helminth taxa | Host (s) | Locality (CS-Code) |
N | Site (s) of infection | P (%) | MI±SD | CNHE (Number of specimens) | Reference |
---|---|---|---|---|---|---|---|---|
Adult Trematoda | ||||||||
Family Allocreadiidae Looss, 1902 | ||||||||
Paracreptotrema blancoisensu |
P. balsanus | (17-Labr) | 29 | Intestine | 50 | 4.8 ± 4.2 | 7687 (15) |
|
(18-Carr) | 25 | Intestine | 8 | 1.0 ± 0 | 7688 (1) |
|
||
(19-Flor) | 20 | Intestine | 25 | 1.0 ± 0 | 7689 (3) |
|
||
(20-Fmag) | 18 | Intestine |
44.4 | 2.2 ± 1.03 | 7690 (4) |
|
||
8 | Intestine | 75 | 1.5 ± 0.5 | This study | ||||
(21-Pich) | 22 |
Intestine | 59 | 2.0 ± 1.3 | 7691 (12) |
|
||
4 | Intestine | 100 | 1.5 ± 0.5 | This study | ||||
(22-Refo) | 20 | Intestine | 20 | 1.7 ± 0.9 | 7692 (3) |
|
||
(23-Viej) | 20 |
Intestine |
10.0 |
1.5 ± 0.7 |
7686 (1) |
|
||
10 | Intestine | 70 | 6.4 ± 8.8 | This study | ||||
(24-Huat) | 7 |
Intestine |
71.4 |
1.8 ± 0.4 |
7694 (3) |
|
||
5 | Intestine | - | - | This study | ||||
(29-Caho) | Intestine | NR | NR |
|
||||
(30-Tama) | Intestine | NR | NR |
|
||||
(31-Agua) | Intestine | NR | NR |
|
||||
(54-Plat) | 4 | Intestine | 100 | 4 ± 4.2 | This study | |||
(55-Jami) | 7 | Intestine | 57.14 | 1.2 ± 0.5 | This study | |||
P. oaxacae | (25-Macu) | 37 | Intestine | 37.8 | 2.6 ± 2.1 | 7693 (5) |
|
|
(32-Sabi) | Intestine | NR | NR | 9286 (2) |
|
|||
Profundulus sp. 2 | (26-Sjri) | 43 | Intestine | 30.2 | 2.5 ± 2.2 | 7684 (7) |
|
|
2 | Intestine | 100 | 3.5 ± 0.7 | This study | ||||
(35-Rchi) | Intestine | NR | NR |
|
||||
P. punctatus | (27-Ojag) | 30 | Intestine | 6.6 | 5.5 ± 4.9 | 7685 (4) |
|
|
(33-Chac) | Intestine | NR | NR |
|
||||
(42-Rnan) | Intestine | NR | NR |
|
||||
(56-Obre) | 8 | Intestine | 50 | 3 ± 1.4 | This study | |||
(58-Tpic) | 15 | Intestine | 13.33 | 3.5 ± 0.7 | This study | |||
(59-Huix) | 20 | Intestine | 20 | 2.25 ± 1.8 | 9803 (2) | This study | ||
(60-Triu) | 6 | Intestine | 33.33 | 1.5 ± 0.7 | 9804 (2) | This study | ||
(61-Fran) | 15 | Intestine | 6.66 | NR | This study | |||
(62-Nahu) | 1 | Intestine | 100 | 1 ± 0 | This study | |||
(63-Prim) | 9 | Intestine | 66.66 | 6.25 ± 3.4 | This study | |||
P. guatemalensis | (44-Rnil) | Intestine | NR | NR |
|
|||
(45-Cant) | Intestine | NR | NR |
|
||||
(47-Rcau) | Intestine | NR | NR |
|
||||
(64-Escu) | 19 | Intestine | 50 | 2.1 ± 1.5 | This study | |||
(65-Sara) | 1 | Intestine | 100 | 1 ± 0 | This study | |||
(66-Sans) | 6 | Intestine | 100 | 4.8 ± 4.6 | This study | |||
P. kreiseri | (49-Qtec) | Intestine | NR | NR |
|
|||
(69-Qpar) | 28 | Intestine | 14.28 | 1.75 ± 1.5 | This study | |||
P. labialis | (57-Espe) | 15 | Intestine | 6.66 | NR | This study | ||
P. portillorum | (70-Lpot) | 9 | Intestine | 11.11 | 1 ± 0 | This study | ||
P. candalarius | (71-Rcar) | 14 | Intestine | 78.57 | 4.3 ± 4.9 | This study | ||
Remarks: Specimens of Paracreptotrema blancoisensu |
||||||||
Paracreptotrema profundulusi Salgado-Maldonado, Caspeta-Mandujano & Martínez Ramírez, 2011. (Fig. |
Profundulus sp. 2 | (26-Sjri) | 43 | Intestine | 55.8 | 4 ± 5.7 | 7680 (1) 7681 (23) |
|
2 | Intestine | 100 | 3.5 ± 0.7 | 9805 (1) | This study | |||
(35-Rchi) | NR | Intestine | NR | NR | 9287 (1) |
|
||
P. punctatus | (27-Ojag) | 30 | Intestine | 20 | 6.8 ± 13.8 | 7682 (6) |
|
|
(33-Chac) | NR | Intestine | NR | NR |
|
|||
P. balsanus | (19-Flor) | 20 | Intestine | 5 | 4 ±0 | 7683 (4) |
|
|
(29-Caho) | NR | Intestine | NR | NR |
|
|||
P. oaxacae | (32-Sabi) | NR | Intestine | NR | NR | 9288 (1) |
|
|
Allocreadiidae gen. sp. | P. portillorum | (70-Lpot) | 9 | Intestine | 11.11 | 1 ± 0 | This study | |
Remarks: A single specimen was collected for future molecular studies. | ||||||||
Family Gorgoderidae Looss, 1901 | ||||||||
Phyllodistomum inecoli Razo-Mendivil, Pérez-Ponce de León & Rubio-Godoy, 2013. (Fig. |
Profundulus sp. 2 | (26-Sjri) | 2 | Urinary bladder | 50 | NR |
|
|
P. punctatus | (56-Obre) | 15 | Urinary bladder | NR | NR |
|
||
(61-Fran) | 15 | Urinary bladder | 6.66 | NR | 9302 (1) |
|
||
P. candalarius | (71-Rcar) | 14 | Urinary bladder | 7.14 | 1 ± 0 | 9802 (1) | This study | |
(72-Lglo) | 22 | Urinary bladder | 31.81 | 1.5 ± 0.7 | 9661 (1) | This study | ||
Remarks: This species was originally recorded by |
||||||||
Phyllodistomum spinopapillatum Pérez-Ponce de León, Pinacho-Pinacho, Mendoza-Garfias & García-Varela, 2015. (Fig. |
P. balsanus | (18-Carr) | 25 | Urinary bladder | 20 | 1 ±0 | 9667 (5) |
|
(20-Fmag) | 18 | Urinary bladder | 5.55 |
1 ±0 |
9666 (1) |
|
||
8 | Urinary bladder | 25 | 1 ± 0 | 9671 (1) | This study | |||
(21-Pich) | 22 | Urinary bladder | 4.54 | 1 ±0 | 9668 (1) |
|
||
(22-Refo) | 20 | Urinary bladder | 10 | 1 ±0 |
|
|||
(23-Viej) | 20 |
Urinary bladder | 40 |
1.12 ±0.35 |
9299 (1) 9300 (7) |
|
||
10 | Urinary bladder | 70 | 1.8 ± 01.5 | This study | ||||
Remarks: This species was recorded as Phyllodistomum sp. by |
||||||||
Family Haploporidae Nicoll, 1914 | ||||||||
Saccocoelioides lamothei Aguirre-Macedo & Violante-González, 2008. (Fig. |
P. balsanus | (18-Carr) | 25 | Intestine | 80 | 3.15 ±2.15 | 9806 (1) |
|
(19-Flor) | 20 | Intestine | 30 | 1.5 ±0.83 |
|
|||
(20-Fmag) | 18 | Intestine | 11.11 | 1 ±0 |
|
|||
8 | Intestine | 12.5 | 1 ± 0 | This study | ||||
(55-Jami) | 7 | Intestine | 42.85 | 5.3 ± 2.5 | This study | |||
Remarks: |
||||||||
Larval Trematoda | ||||||||
Family Clinostomidae Lühe, 1901 | ||||||||
Clinostomum sp. (Fig. |
P. punctatus | (13-Ocot) | 12 | Mesentery | 8.3 | 1±0.0 | 7442 (1) |
|
(60-Triu) | 6 | Mesentery | 16.66 | NR | This study | |||
P. balsanus | (18-Carr) | 25 | Mesentery, Gills, eyes, Body cavity |
40 | 2.7 ±1.94 | 9202 (5) |
|
|
(20-Fmag) | 18 | Mesentery, Gills, eyes, Body cavity |
5.55 | 1 ±0 |
|
|||
(22-Refo) | 20 | Mesentery, Gills, eyes, Body cavity |
10 | 1 ±0 |
|
|||
(23-Viej) | 20 | Mesentery, Gills, eyes, Body cavity |
10 | 1.5 ±0.70 |
|
|||
(24-Huat) | 7 |
Mesentery, Gills, eyes, Body cavity |
42.85 |
1 ±0 |
|
|||
5 | Mesentery | NR | NR | This study | ||||
(54-Plat) | 4 | Mesentery | 25 | 3 ± 0 | This study | |||
(55-Jami) | 7 | Mesentery | 14.28 | 1 ± 0 | 9660 (1) | This study | ||
P. candalarius | (71-Rcar) | 14 | Mesentery | 21.42 | 2 ± 0 | This study | ||
Remarks: This species was recorded as Clinostomum complanatum by |
||||||||
Family Diplostomidae Poirier, 1886 | ||||||||
Diplostomidae gen. sp. (Fig. |
P. balsanus | (18-Carr) | 25 | Mesentery | 4 | 1 ±0 |
|
|
(22-Refo) | 20 | Mesentery | 5 | 4 ±0 |
|
|||
Posthodiplostomum minimum MacCallum, 1921. (Fig. |
P. balsanus | (22-Refo) | 20 | Mesentery | 25 | 2.6 ±1.34 |
|
|
(23-Viej) | 20 | Mesentery | 5 | 1 ± 0 |
|
|||
10 | Mesentery | 10 | 2 ± 0 | 9807 (1) | This study | |||
(24-Huat) | 7 | Mesentery | 14.28 | 4 ± 0 |
|
|||
5 | Mesentery | NR | NR | This study | ||||
Family Heterophyidae Leiper, 1909 | ||||||||
Ascocotyle (Ascocotyle) felippei Travassos, 1928. (Fig. |
P. balsanus | (18-Carr) | 25 | Heart | 28 | 165.42 ±72.39 | 9199 (10) |
|
(19-Flor) | 20 | Heart | 20 | 23.75 ±21.96 |
|
|||
(20-Fmag) | 18 | Heart | 83.33 | 16.73 ±15.07 |
|
|||
(21-Pich) | 22 | Heart | 86.36 | 58.94 ±43.31 | 9200 (6) |
|
||
(22-Refo) | 20 | Heart | 60 | 7.25 ±10.48 |
|
|||
(23-Viej) | 20 | Heart | 95 | 61.84 ±77.81 |
|
|||
(24-Huat) | 7 | Heart | 14.28 | 6 ±0 |
|
|||
P. punctatus | (63-Prim) | 9 | Heart | 11.11 | NR | This study | ||
Centrocestus formosanus Nishigori, 1924. (Fig. |
P. balsanus | (20-Fmag) | 18 | Gills | 72.22 | 12.15 ±21.57 |
|
|
(21-Pich) | 22 | Gills | 100 | 821.63 ±417.59 | 9201 (3) |
|
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(22-Refo) | 20 | Gills | 100 | 42.45 ±33.39 |
|
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(23-Viej) | 20 | Gills | 5.88 | 31 ±0 |
|
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(24-Huat) | 7 | Gills | 50 | 1.66 ±1.15 |
|
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P. punctatus | (62-Nahu) | 1 | Gills | 100 | 3 ± 0 | This study | ||
(63-Prim) | 9 | Gills | 11.11 | NR | This study | |||
Monogenea | ||||||||
Family Gyrodactylidae van Beneden & Hesse, 1863 | ||||||||
Gyrodactylus sp. (Fig. |
P. balsanus | (19-Flor) | 20 | Fins | 5 | 1 ±0 |
|
|
(22-Refo) | 20 | Fins | 5 | 2 ±0 |
|
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(23-Viej) | 20 | Fins | 10 | 1 ±0 |
|
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Remarks: The limited number of specimens found precluded the specific identification of this species; however, based on their morphology they clearly represent members of Gyrodactylus. | ||||||||
Family Dactylogyridae Bychowsky, 1937 | ||||||||
Urocleidoides sp. (Fig. |
P. balsanus | (19-Flor) | 20 | Gills | 5 | 1 ±0 |
|
|
(20-Fmag) | 18 | Gills | 72.22 | 7.15 ±6.37 |
|
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(21-Pich) | 22 | Gills | 13.63 | 3 ±1.73 |
|
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(22-Refo) | 20 | Gills | 75 | 7 ±5.45 |
|
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(23-Viej) | 20 | Gills | 82.35 | 5.85 ±5.27 |
|
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(24-Huat) | 7 | Gills | 83.33 | 7.4 ±4.44 |
|
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P. punctatus | (62-Nahu) | 1 | Gills | 100 | 1 ± | This study | ||
P. guatemalensis | (64-Escu) | 19 | Gills | 5.26 | 4 ± | This study | ||
(65-Sara) | 1 | Gills | 100 | 1 ± | This study | |||
Remarks: |
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Adult Cestoda | ||||||||
Family Bothriocephalidae Blanchard, 1849 | ||||||||
Bothriocephalus acheilognathi Yamaguti, 1934. (Fig. |
P. hildebrandi | (3-Ecos) | 234 | Intestine | 54 | 4.62±2.38 | 7617 (2) |
|
(4-Alba) | 168 | Intestine | 61 | 13.10±8.57 |
|
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(5-5mar) | 173 | Intestine | 59 | 4.35 ± 2.51 |
|
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(6-Puen) | 85 | Intestine | 2 | 1±0.00 |
|
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(7-Cham) | 126 | Intestine | 41 | 1.88±0.55 |
|
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(8-Peor) | 128 | Intestine | 71 | 6.34±2.48 |
|
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(9-Arco) | 64 | Intestine | 11 | 2.57±4.48 |
|
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(10-Aren) | 64 | Intestine | 6 | 1±0.00 |
|
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(11-Paja) | 141 | Intestine | 5 | 1.14±0.76 |
|
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(12-Lsoy) | 4 | Intestine | 100 | 4.50±4.04 |
|
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(73-Moxv) | 20 | Intestine | 20 | 11 ± 9.9 | This study | |||
(74-Poro) | 7 | Intestine | 42.85 | NR | This study | |||
P. portillorum | (51-Ojoj) | 30 | Intestine | NR | NR | 9368 |
|
|
P. guatemalensis | (66-Sans) | 6 | Intestine | 16.66 | 4 ± | 9670 (1) | This study | |
P. candalarius | (72-Lglo) | 22 | Intestine | 22.72 | 1.6 ± 1.1 | 9669 (1) | This study | |
(39- Ateo) | NR | Intestine | NR | NR |
|
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Remarks: |
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Adult Nematoda | ||||||||
Family Cystidicolidae Skrjabin, 1946 | ||||||||
Spinitectus humbertoi Caspeta-Mandujano & Moravec, 2000. (Fig. |
P. balsanus | (1-Inzc) | 1 | Intestine | 100 | NR | 4028 (1) 4030 (2) |
|
(23-Viej) | 20 | Intestine | 10 | 6.5 ±3.53 |
|
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(24-Huat) | 7 | Intestine | 14.28 | 17 ±0 |
|
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5 | Intestine | NR | NR | 9443 (5) | This study | |||
(28-Rlac) | NR | Intestine | NR | NR |
|
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(29-Caho) | NR | Intestine | NR | NR |
|
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(30-Tama) | NR | Intestine | NR | NR |
|
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P. punctatus | (33-Chac) | NR | Intestine | NR | NR |
|
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(40-Rsan) | NR | Intestine | NR | NR |
|
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Profundulus sp. 1 | (34-Chic) | NR | Intestine | NR | NR |
|
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P. labialis | (40-Rsan) | NR | Intestine | NR | NR |
|
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(41-Atpi) | NR | Intestine | NR | NR |
|
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(42-Rnan) | NR | Intestine | NR | NR |
|
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P. guatemalensis | (44-Rnil) | NR | Intestine | NR | NR |
|
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(45-Cant) | NR | Intestine | NR | NR |
|
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(47-Rcau) | NR | Intestine | NR | NR |
|
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(48-Cauc) | NR | Intestine | NR | NR |
|
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(66-Sans) | 6 | Intestine | 50 | 3.3 ± 2 | 9639 (5) | This study | ||
P. kreiseri | (50-Nono) | NR | Intestine | NR | NR |
|
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P. portillorum | (70-Lpot) | 9 | Intestine | 22.22 | NR | 9638 (5) | This study | |
P. candalarius | (71-Rcar) | 14 | Intestine | 57.14 | 2.6 ± 2.5 | This study | ||
Remarks: This nematode was originally described from the intestine of Profundulus labialis in Guerrero, Mexico. Apparently, the type host was erroneously identified by |
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Spinitectus mariaisabelae Caspeta-Mandujano Cabañas-Carranza & Salgado-Maldonado, 2007 | P. punctatus | (2-Such) | NR | Intestine | NR | NR | 5781(1)5783 (6) |
|
(16-Saju) | 3 | Intestine | 100 | 3.3±2.0 |
|
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(13-Ocot) | 12 | Intestine | 100 | 5.7±2.9 |
|
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P. labialis | (14-Chic) | 3 | Intestine | 100 | 4.0±3.0 |
|
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(15-Rsuc) | 24 | Intestine | 79.2 | 4.3±3.2 |
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(13-Ocot) | 3 | Intestine | 100 | 2.6±2.8 |
|
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Family Rhabdochonidae Travassos, Artigas & Pereira, 1928 | ||||||||
Rhabdochona salgadoi Caspeta-Mandujano & Moravec, 2000. (Fig. |
P. balsanus | (1-Inzc) | 1 | Intestine | 100 | NR | 4031 (1) 4033 (32) |
|
(18-Carr) | 25 | Intestine | 60 | 4.4 ±4.15 |
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(19-Flor) | 20 | Intestine | 70 | 5.71 ±4.95 |
|
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(20-Fmag) | 18 | Intestine | 83.33 | 4.46 ±3.11 |
|
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(21-Pich) | 22 | Intestine | 54.54 | 2.75 ±1.86 |
|
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(22-Refo) | 20 | Intestine | 95 | 8.05 ±3.99 |
|
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(23-Viej) | 20 | Intestine | 90 | 7.66 ±4.95 |
|
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(24-Huat) | 7 | Intestine | 100 | 18.57 ±10.84 |
|
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(28-Rlac) | NR | Intestine | NR | NR |
|
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(29-Caho) | NR | Intestine | NR | NR |
|
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(30-Tama) | NR | Intestine | NR | NR |
|
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(31-Agua) | NR | Intestine | NR | NR |
|
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P. oaxacae | (32-Sabi) | NR | Intestine | NR | NR |
|
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P. punctatus | (33-Chac) | NR | Intestine | NR | NR |
|
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(40-Rsan) | NR | Intestine | NR | NR |
|
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(13-Ocot) | 12 | Intestine | 66.7 | 3.2±1.9 |
|
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(43-Cane) | NR | Intestine | NR | NR |
|
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(59-Huix) | 20 | Intestine | 30 | NR | 9637 (5) | This study | ||
(62-Nahu) | 1 | Intestine | 100 | NR | This study | |||
(63-Prim) | 9 | Intestine | 22.22 | NR | This study | |||
P. labialis | (15-Rsuc) | 24 | Intestine | 58.3 | 2.6±1.7 |
|
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(40-Rsan) | NR | Intestine | NR | NR |
|
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(41-Atpi) | NR | Intestine | NR | NR |
|
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Profundulus sp. 2 | (35-Rchi) | NR | Intestine | NR | NR |
|
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(13-Ocot) | 3 | Intestine | 33.3 | 6.0±0 |
|
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Profundulus sp. 1 | (34-Chic) | NR | Intestine | NR | NR |
|
||
P. candalarius | (37-Rcom) | NR | Intestine | NR | NR |
|
||
(71-Rcar) | 14 | Intestine | 7.14 | 1 ± | 9640 (5) | This study | ||
(72-Lglo) | 22 | Intestine | 4.54 | 1 ± | This study | |||
P. guatemalensis | (44-Rnil) | NR | Intestine | NR | NR |
|
||
(64-Escu) | 19 | Intestine | 21.05 | NR | 9642 (5) | This study | ||
(65-Sara) | 1 | Intestine | 100 | NR | This study | |||
(67-Rhon) | 6 | Intestine | 16.66 | NR | This study | |||
P. kreiseri | (49-Qtec) | NR | Intestine | NR | NR | 9290 (5) |
|
|
(68-Cham) | 6 | Intestine | 83.33 | NR | 9641 (5) | This study | ||
(69-Qpar) | 28 | Intestine | 71.42 | NR | This study | |||
(50-Nono) | NR | Intestine | NR | NR |
|
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P. portillorum | (52-Lepa) | NR | Intestine | NR | NR |
|
||
Larval Nematodes | ||||||||
Family Anisakidae Railliet & Henry, 1912 | ||||||||
Contracaecum sp. | P. punctatus | (13-Ocot) | 12 | Intestine | 8.3 | 1.0±0 |
|
|
(59-Huix) | 20 | Mesentery | 5 | NR | This study | |||
(60-Triu) | 6 | Mesentery | 16.66 | NR | 9808 (1) | This study | ||
Family Dioctophymatidae Railliet, 1915 | ||||||||
Eustrongylides sp. (Fig. |
P. punctatus | (13-Ocot) | 12 | Intestine | 41.6 | 1.6±0.5 |
|
|
P. balsanus | (19-Flor) | 20 | Mesentery | 5 | 1 ±0 |
|
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(20-Fmag) | 18 | Mesentery | 16.66 | 2 ±1 |
|
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P. candalarius | (71-Rcar) | 14 | Mesentery | 7.14 | NR | 9809 (1) | This study | |
Family Gnathostomatidae Railliet, 1895 | ||||||||
Spiroxys sp. | P. portillorum | (70- Lpot) | 9 | Intestine | 11.11 | NR | 9810 (1) | This study |
Species of trematodes found in Profundulus spp. A Paracreptotrema blancoi sensu
Host | Helminth parasite | Reference |
---|---|---|
Profundulus balsanus Ahl, 1935 |
Digenea Ascocotyle (Ascocotyle) felippei (M) Centrocestus formosanus (M) Clinostomum sp. (M) Diplostomidae gen. sp. (M) Phyllodistomum spinopapillatum (A) Posthodiplostomum minimum (M) Paracreptotrema blancoi (A) Paracreptotrema profundulusi (A) Saccocoelioides lamothei (A) Monogenea Gyrodactylus sp. (A) Urocleidoides sp. (A) Nematoda Eustrongylides sp. (L) Rhabdochona salgadoi (A) Spinitectus humbertoi (A) |
This study This study This study This study This study This study |
Profundulus candalarius Hubbs, 1924 |
Digenea Clinostomum sp. (M) Phyllodistomum inecoli (A) Paracreptotrema blancoi (A) Cestoda Bothriocephalus acheilognathi (A) Nematoda Eustrongylides sp. (L) Spinitectus humbertoi (A) |
This study This study This study This study This study This study |
Profundulus guatemalensis (Günther, 1866) |
Digenea Paracreptotrema blancoi (A) Monogenea Urocleidoides sp. (A) Cestoda Bothriocephalus acheilognathi (A) Nematoda Rhabdochona salgadoi (A) Spinitectus humbertoi (A) |
This study This study This study This study This study |
Profundulus hildebrandi Miller, 1950 |
Cestoda Bothriocephalus acheilognathi (A) |
This study |
Profundulus kreiseri Matamoros, Schaefer, Hernández & Chakrabarty, 2012 |
Digenea Paracreptotrema blancoi (A) Nematoda Rhabdochona salgadoi (A) Spinitectus humbertoi (A) |
This study This study |
Profundulus labialis (Günther, 1866) |
Digenea Paracreptotrema blancoi (A) Nematoda Rhabdochona salgadoi (A) Spinitectus humbertoi (A) Spinitectus mariaisabelae (A) |
This study |
Profundulus oaxacae (Meek, 1902) |
Digenea Paracreptotrema blancoi (A) Paracreptotrema profundulusi (A) Nematoda Rhabdochona salgadoi (A) |
|
Profundulus portillorum Matamoros & Schaefer, 2010 |
Digenea Paracreptotrema blancoi (A) Allocreadiidae gen. sp. (A) Cestoda Bothriocephalus acheilognathi (A) Nematoda Rhabdochona salgadoi (A) Spinitectus humbertoi (A) Spiroxys sp. (L) |
This study This study This study This study |
Profundulus punctatus (Günther, 1866) |
Digenea Ascocotyle (Ascocotyle) felippei (M) Centrocestus formosanus (M) Clinostomum sp. (M) Phyllodistomum inecoli (A) Paracreptotrema blancoi (A) Paracreptotrema profundulusi (A) Monogenea Urocleidoides sp. (A) Nematoda Contracaecum sp. (L) Eustrongylides sp. (L) Rhabdochona salgadoi (A) Spinitectus humbertoi (A) Spinitectus mariaisabelae (A) |
This study This study This study This study This study This study This study |
Profundulus sp. 1 |
Nematoda Rhabdochona salgadoi (A) Spinitectus humbertoi (A) |
|
Profundulus sp. 2 |
Digenea Phyllodistomum inecoli (A) Paracreptotrema blancoi (A) Paracreptotrema profundulusi (A) Nematoda Rhabdochona salgadoi (A) |
This study This study |
Digeneans exhibit the highest species richness (11 species), followed by nematodes (six species) and monogeneans (two species) (Table
The most widely distributed parasites among profundulids are the nematodes R. salgadoi and S. humbertoi, and the digenean P. blancoisensu
In terms of the species richness of the helminths in relation to the host species, Profundulus balsanus is the species with the highest diversity, since it is parasitized by 14 species, followed by P. punctatus with 12, and P. candalarius and P. portillorum with six (Table
Fish were collected at 26 localities in southern Mexico, Guatemala and Honduras, and a total of 267 individual fish belonging to nine species of Profundulus was examined for helminth parasites. The inventory was completed by adding these records to the previous parasite surveys conducted on members of this fish group endemic to Middle-America. Interestingly, the number of individual hosts studied for helminths of this fish group has increased significantly during a two-year period across the entire distributional range, and it seems that only two new species were found. Firstly, a detailed morphological evaluation of the specimens recorded herein as Urocleidoides sp. indicate they represent an undescribed species, which will be formally described in a separate paper. A thorough revision of the morphology of the specimens identified as the trematode Paracreptotrema blancoi by
Six adult helminth species are considered to be part of the biogeographical ‘core’ helminth fauna of profundulids. As discussed by
This represents the second complete inventory of a freshwater fish group.
There are, however, some notable differences between the biogeography of the Goodeinae and Profundulidae. The Goodeinae is an endemic fish component of central and northern Mexico which experienced an important adaptive radiation and contains 45 species (
Irrespective of the biogeographical history of the ancestor of both profundulids and goodeids, and the subsequent radiation of the latter, the former did not diversify in the same way as goodeids did. Adaptive radiation of goodeines in central Mexico, following a complex geological and hydrological history (see
As suggested by
We are grateful to Carla Mena, Eufemia Arenas, and Marly Martínez for their help during field work. We thank B. Mendoza-Garfías for processing some of the specimens, and L. García-Prieto for providing literature and specimens for morphological comparison deposited at the CNHE. This research was supported by grants from the Programa de Apoyo a Proyectos de Investigación e Inovación Tecnológica (PAPIIT-UNAM) IN207213 and IN219904 to MGV and GPPL, respectively, and the Consejo Nacional de Ciencia y Tecnología (CONACYT) 179048 to MGV. C.D.P.P. thanks the support of the Programa de Posgrado en Ciencias Biológicas, UNAM and CONACYT for granting a scholarship to complete his PhD program. JSHO and C.A.M. benefited from a Postdoctoral Fellowship of the Dirección General Asuntos del Personal Académico (DGAPA-UNAM).