Review of the New World Notomicrus Sharp (Coleoptera, Noteridae) I: Circumscription of species groups and review of the josiahi group with description of a new species from Brazil

Stephen M. Baca; Andrew Edward Z. Short

doi:10.3897/zookeys.1025.60442

Research Article

Review of the New World Notomicrus Sharp (Coleoptera, Noteridae) I: Circumscription of species groups and review of the josiahi group with description of a new species from Brazil

Stephen M. Baca^‡, Andrew Edward Z. Short^‡

‡ University of Kansas, Lawrence, United States of America

Corresponding author: Stephen M. Baca ( baca@ku.edu )

Academic editor: Mariano Michat

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation: Baca SM, Short AEZ (2021) Review of the New World Notomicrus Sharp (Coleoptera, Noteridae) I: Circumscription of species groups and review of the josiahi group with description of a new species from Brazil. ZooKeys 1025: 177-201. https://doi.org/10.3897/zookeys.1025.60442

ZooBank: urn:lsid:zoobank.org:pub:C9D007A0-2385-4104-816C-F9177473ABBC

Abstract

The New World species of the minute aquatic beetle genus Notomicrus Sharp compose a much greater diversity than their Old World congeners, with 14 of the 17 known Notomicrus species occurring in the Neotropics. A recent phylogenetic study recovered four primary New World species groups and found that there are a number of undescribed species across all of these main lineages. Here, we provide a taxonomic key to these New World species groups, including two described species that we currently do not place in any group (“incertae sedis” species), complete with images and illustrations of diagnostic characters and taxonomic notes including a list of known species in each group. This work provides a scaffold for further planned taxonomic revisions within the genus. In addition, we review the first of the four New World groups, the josiahi species group and describe one new taxon, N. interstinctus sp. nov. from northern Brazil. Provided are descriptions, habitus images and illustrations of diagnostic characters.

Keywords

Aquatic beetles, Brazil, new species, South America, taxonomy

Introduction

Notomicrus Sharp is the most speciose genus of the minute aquatic beetle subfamily Notomicrinae (Coleoptera: Noteridae). Its distribution spans Indomalaya, Oceania and the New World, though the majority of Notomicrus diversity occurs in the Neotropics (14 of 17 described species). Notomicrus species occupy a wide range of habitats, including the margins of ponds, streams, marshes and swamps, drying stream beds, forest pools, hygropetric habitats and terrestrial leaf litter. Some species present a high specificity in their habitat preference, while others are found to be more generalists (Baca and Short 2020; personal observation). This ecological plasticity is a quality of the subfamily Notomicrinae as a whole. Both of the other notomicrine genera are subterranean specialists: Phreatodytes Uéno from aquifers in Japan and Speonoterus Spangler, a monotypic genus known only from a single collection in a shallow cave in Indonesia. Speonoterus appears to be a very close relative of Notomicrus, with Phreatodytes being their sister-group (Baca et al. 2017; Baca and Short 2020). It has been speculated that, given the plasticity of the habit preferences of Notomicrus and the aforementioned morphological similarity, Speonoterus may represent a specialized Notomicrus species (Baca and Short 2020). These relationships remain to be tested with molecular sequence data as Speonoterus is known only from the few specimens of the type series (Spangler 1996).

Since its establishment by Sharp (1882), the classification of Notomicrus and its species has been very stable. Notomicrus nanulus (LeConte, 1863) and Notomicrus tenellus (Clark, 1863) are the only species that have required nomenclatural recombination as these were described before the genus was established, thus necessitating transfer (Nilsson 2011; note that some synonyms of N. tenellus were also described before the erection of Notomicrus). Few junior synonyms of any Notomicrus species have been described (Nilsson 2011), and the genus itself has no current generic synonyms. Classification changes have occurred at higher levels, for example, tribe, subfamily and family levels, but the genus status of Notomicrus has remained unaltered.

The monophyly of Notomicrus has been previously supported, with the Old and New World clades each also being found to represent reciprocally monophyletic lineages (Baca et al. 2017; Baca and Short 2020). These studies have also revealed that, unsurprisingly, there remain many undescribed species in the genus, especially in South America. This is further indicated by recent descriptions of new Neotropical species (Miller 2013; Manuel 2015; Baca and Short 2018; Guimarães and Ferreira-Jr 2019). Together, these works have greatly strengthened our understanding of notomicrine diversity and evolution. However, in effect, Young’s (1978) benchmark revision of the New World Notomicrus now includes just over half of the currently described species, amplifying the need for a comprehensive treatment of the group, especially with more diversity remaining to be described.

The species-level phylogenetic reconstruction of Baca and Short (2020) placed heavy emphasis on New World Notomicrus diversity. They recovered New World Notomicrus as diverging into four clades, the josiahi, nanulus, meizon and traili species groups, reciprocally supported by morphological characters. As such, Baca and Short (2020) provide an appropriate scaffold for taxonomic treatment of the groups.

Here, we (1) diagnose and provide a taxonomic key to the four primary species groups of New World Notomicrus. As part of this objective, we review morphological characters of importance, illustrate diagnostic characters and provide habitus images of exemplar species, taxonomic notes and a list of known species and references for each group. We then (2) present the first of four species-level revisionary works of New World Notomicrus by reviewing the josiahi species group. Included are a diagnosis of the group, a re-description of N. josiahi Miller, 2013 and a description of a new species from Brazil.

Materials and methods

Observations and measurements

Specimens were observed and measured using an Olympus SZX7 stereomicroscope. The microscope was equipped with 10× eyepieces, a DF PL 2×_-4 objective (16–112× magnification) and a calibrated ocular micrometer. Genitalia and tarsal claws were relaxed in hot water and dissected. Dissections were placed in glycerine on glass slides for observation. For additional observations and images of the prosternal process, aedeagi and tarsal claws, selected specimens were cleared in a warm 10% potassium hydroxide (KOH) solution and periodically checked multiple times an hour. Once desired elimination of soft tissue was achieved, specimens were thoroughly rinsed in DI (deionized) water. In some cases, DNA voucher specimens were used for observation and imaging of structures as the lysing process also dissolves soft tissue, effectively clearing the specimen and negating the need to damage additional specimens.

Images and illustrations

Dorsal habitus images were obtained with a Visionary Digital microphotography system equipped with an Infinity K2 microscope using a 5× objective and Helicon Focus imaging software. Photos were aligned and stacked using CombineZP (www.hadleyweb.pwp.blueyonder.co.uk) and refined in Adobe Photoshop. Ventral images and images of structures to be used for illustrations were taken with an Olympus DP72 camera system attached to either an Olympus SZX16 stereomicroscope with an SDF PLAPO 1×PF or 2×PF objective or an Olympus BX51 compound microscope with an UPlanFLN 40× oil immersion objective. The digital images were then stacked as above, with structures traced using Adobe Illustrator. Prolegs, prosterna, noterid platforms and male genitalia were imaged with the aforementioned stereomicroscope imagining system; illustrations were traced from these images. Male genitalia were placed in a depression slide with a drop of KY jelly and the remainder of the depression was filled with ethanol (EtOH). The KY jelly maintains its viscosity so that genitalia will hold its position for imaging. The EtOH eliminates obscuring refraction. Tarsal claws were imaged on the compound microscope. The fifth (V) pro- and metatarsomeres with tarsal claws were placed on a flat slide with EtOH and a cover slip was applied and glycerine was then used the seal the outside of the slip. The lower surface tension of the EtOH allows the cover slip to press on the claws, flattening them against the slide.

Terminology

Descriptive terminology follows previous works (e.g. Manuel 2015; Baca and Short 2018).

Noterid platform. In Notomicrus, the noterid platform is formed by the raised projections of the inner metacoxal lamellae.

Genitalia and appendages. Following Miller and Nilsson (2003), genitalia and appendages are described in their fundamental homologous positions.

List of depositories

INPA Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil (N. Hamada);

MIZA Museo del Instituto de Zoología Agrícola, Maracay, Venezuela (L. Joly);

MSB Museum of Southwestern Biology, University of New Mexico (K. Miller);

NHM Natural History Museum, London, UK (M. Barclay, C. Taylor);

SEMC Snow Entomological Collection, University of Kansas, Lawrence, KS (A. Short);

USNM U.S. National Museum of Natural History, Smithsonian Institution, Washington, DC (C. Micheli).

Structures of taxonomic importance for diagnoses of Notomicrus species

Size. The total body length of Notomicrus species ranges between ca. 1.0 mm and 1.8 mm. Following Young (1978), size can, in combination with other characters, be very helpful in species determination. Size is quantified in terms of (1) total length (TL), as measured from anterior margin of head to apex of elytra, in dorsal aspect, (2) total length without head (TLPn), measured from medial anterior margin of pronotum to elytral apex (this is included to provide a consistent length measurement, as the degree to which the head is ventrally reflexed can affect the TL measurement) and (3) greatest width (GW), as measured transversely at the widest point of the beetle. Means of the measurements for each species, with standard deviations (SD) of the mean are also presented. Ratios of TL and GW are given as a way of quantifying the shape of the body outline. Means of the measurements for each species, with standard deviations (SD) of the mean for TL are also presented. Ratios of TL and GW are given as a way of quantifying the shape of the body outline.

Color. Most species of Notomicrus present dorsal coloration as varying shades from brown to yellow. However, individuals of some species present specific color patterns among sclerites. For example, some species appear bicolorous, with the elytra and head darker brown and contrasting against a lighter colored pronotum, for example, N. traili Sharp, 1882 (Fig. 1d, f). Other species, such as N. nanulus (LeConte, 1863) (Fig. 1a–c), are more uniformly brown, with little contrast between elytra, head and pronotum. Others still, such as N. josiahi Miller, 2013, present elytra with dark areas distinctly contrasting against lighter areas and/or may have a notable iridescent sheen (Fig. 5). Color patterns of the ventral surface can also be helpful in delimiting species. Color is best used in conjunction with other characters, as many species share similar coloration. Intraspecific variation is often present, with individuals appearing relatively lighter or darker in color, this variation being additionally present between mature and teneral individuals.

Punctation. Elytral punctation can be very helpful in diagnosing species of Notomicrus. Many species differ in the relative coarseness, density and patterns of punctation. Punctation should be used in combination with other characters to diagnose species as this character often presents similarly across multiple species.

Microsculpture. External microsculpture in Notomicrus varies among species and, in combination with other characters, can be helpful for diagnosis. In Notomicrus, the microsculpture consists of a microreticulation, where a superficially impressed mesh of very fine lines or grooves creates small cells. This is usually present on most external sclerites of the head, thorax, abdomen and legs, though it may not be uniform across these sclerites in an individual (e.g. the microsculpture of the noterid platform often differs from that of the elytra). In particular, the degree of impression and size or density of the meshes can be characteristic for a species or group of species.

Eye size. The size of the eyes, relative to the head capsule, can be very useful in identifying species. Here, the relative eye size is presented as a ratio of the greatest width of the head (HW) and interocular distance (EW). Measurements are taken from dorsal aspect, approximately at posterolateral margins of the eyes. Interocular distance is taken from the narrowest point between the eyes. The larger the eyes relative to the head capsule, the larger the ratio HW/EW, for example, N. josiahi HW/EW = 2.35–2.53, N. petrareptans Baca & Short, 2018 HW/EW = 1.65–1.73.

Prosternal process. The shape of the prosternal process was observed to be variable among some species and species groups of Notomicrus (Fig. 3). In particular, the shape of the apex and degree of constriction between the procoxae can be diagnostic in combination with other characters. For example, being acutely angled as in N. josiahi (Fig. 3a) or more rounded or blunt, as in N. nanulus (Fig. 3b).

Tarsal claws. The pro- and mesotarsal claws of males of Notomicrus show significant interspecific variation in size and shape. Following Young (1978), the shape of the claws, as well as the relative sizes of the anterior claws and posterior claws can be helpful in diagnosing species. Here we describe and illustrate the claws in lateral view (Figs 8, 9). The finer details of the claws’ shape may be difficult to view without the use of a compound microscope. It should be noted that slide mounting the claws can variably alter the appearance compared to the in situ appearance under a stereomicroscope, this in part being due to their asymmetrical shape or the claws being slightly splayed on dried specimens. Characters of the tarsal claws are best used in combination with other characters.

Aedeagus. The aedeagus is especially helpful for diagnosing species. The median lobe should be observed from several angles as it tends to be asymmetric and an oblique orientation can give the appearance of a different shape. Despite relative reliability, the aedeagus is still best used in combination with other characters for identification. Many species, even those across species groups, can present very similar aedeagi. For example, the aedeagus of N. interstinctus sp. nov. (Fig. 7) converges very closely on members of the traili group. Additionally, the males of some species are unknown, suggesting these lineages may only comprise females, for example, N. femineus Manuel, 2015.

Taxonomy

Genus Notomicrus Sharp, 1882

Type species

N. brevicornis Sharp, 1882. Designation by Guignot 1946: 115.

Diagnosis

(1) Eyes present; (2) metacoxae and metaventrite fused, suture indistinct laterad of noterid platform; (3) noterid platform not extending anteriorly on to metaventrite; (4) protibia with loose rows of spines and setae, lacking large spur at apex and tight comb of small spines on distolateral margin and not expanded distally beyond protarsal insertion; (5) partial fusion of metafurca and metacoxae, not forming complete ring; (6) mid-gular apodeme absent (Beutel and Roughley 1987; Miller 2009); (7) female laterotergite short, posteriorly extending beyond base of gonocoxae (Miller 2009).

Remarks

As noted by Miller (2009) and others (Manuel 2015 and citations therein), the characters that define Notomicrus are primarily plesiomorphic with the exception of the fusion of the metacoxae and metaventrite. Speonoterus Spangler is also defined by the above character combination, except absence of eyes. Spangler (1996) also noted that the distance from the anterior terminus of the noterid platform (metacoxal lamellae) to the mesocoxal cavities is shorter in Speonoterus, less than the width of the mesocoxal cavities, whereas in Notomicrus, this distance is greater than the width of the mesocoxal cavities (See Spangler 1996; Manuel 2015). Notomicrine species are all notably small (ca. 1.0–1.8 mm). Characters listed above without specific citation have been more common in use for defining Notomicrus (e.g. Sharp 1882; Young 1978; Buetel and Roughley 1987); see Miller (2009) and Manuel (2015) for details.

Key to species groups and insertae sedis species of Notomicrus Sharp

This key is intended to be used as a first step in identifying New World species of Notomicrus. Identification of Notomicrus species can prove difficult for non-specialists, especially without additional species in hand for comparisons. Diagnoses of the species groups of Notomicrus also reflect this difficulty.

1	Size small, TL = 1.3 mm. Elytral punctation almost entirely indistinct, except discal row and submargin of elytral suture with distinct punctures, with very fine scattered setose punctures near lateral margins; elytral surface with microreticulation consisting of round, isodiametric cells, somewhat scale-like in appearance. Head appendages short, antennomeres VI–X wider than long; apical palpomeres distinctly bifurcate with enlarged sensory fields. Aedeagus as in Fig. 2 of Guimarães and Ferreira-Jr (2019); median lobe with large base and very large processes and hooks. Male pro- and mesotarsal claws short, anterior protarsal claw expanded at base. Known only from high elevation hygropetric habitats in Minas Gerais, Brazil	*N. teramnus*
–	Size variable, ca. 1.2–1.9 mm. Elytral punctation and microsculpture variable. Antennomeres usually longer than wide; apical palpomeres variable. Median lobe of aedeagus without conspicuous hooks or large processes (e.g. Figs 6, 7). Male pro- and mesotarsal claws variable. Habitat preference variable	2
2	Dorsal (outer) margin of protibia without notable robust seta at or near mid-length (Fig. 2a). Eyes large relative to head capsule (Fig. 5), HW/EW ≥ 2.0. Elytra with notable contrasting dark and light colors (Fig. 5a, c). Profemur with > 3 distinct closely spaced setae on anteroventral margin (Fig. 2a); posteroventral margin of male profemur lacking notable protuberance, only weakly angled near mid-length (Fig. 2a); male protarsal claws very small, distinctly shorter than half the length of protarsomere V, anterior protarsal claw bifurcate, branching dorsally (Figs 8, 9)	josiahi group
–	Dorsal margin of protibia with a robust seta at or near mid-length (often two in females), at least as long as most dorsal seta on dorsoapical angle (Fig. 2b–f). Eyes smaller, HW/HW < 2.0. Elytra with or without contrasting colors. Profemur with < 3 closely-spaced setae on anteroventral margin; posteroventral margin of male profemur with notable protuberance or acute angle at ca. mid-length; male protarsal claws variable, anterior claw length almost always at least half the length of protarsomere V, almost always larger than female claws, sometimes bifurcate	3
3	Noterid platform with angles of posterior lobes squared or rounded (Fig. 4b–e)	4
–	Noterid platform with angles posterior lobes acutely angled (as in Fig. 4a, f–l)	5
4	Elytral surface impunctate to weakly punctate, punctures usually inconspicuous and sporadic under normal magnification, except for discal series; microreticulation variably impressed, consisting of small, round, isodiametric cells, giving the appearance of small scales. Body form variable, but usually oblong, less attenuated posteriorly (Fig. 1a–c). Elytral color uniform, brown, sometimes shiny, not iridescent or only weakly so	nanulus group
–	Punctation distinctly present and often dense on posterior half of elytra, punctures finely to moderately impressed, bearing short setae, often extending on to anterior half of elytra; microreticulation variably impressed, consisting of fine mesh-like reticulation. Body form variable, but more elongate and attenuated posteriorly (Fig. 1g–i). Color variable, but elytra of mature specimens of most species with darker triangular area medially at base (Fig. 1g, i); in most species, dorsal surface very shiny and iridescent	meizon group (in part)
5	Color uniformly brown. Elytral surface with microreticulation variably impressed, consisting of small, round, isodiametric cells, giving the appearance of fine scales, somewhat shiny, but never iridescent; punctation variable. Males with anterior protarsal claw bifurcate or branched (as in Fig. 8a)	6
–	Color variable, uniform or bicolorous. Elytral surface with microreticulation variably impressed, consisting of fine mesh-like reticulation, sometimes iridescent. Males with protarsal claws never bifurcated or branched	7
6	Body form oblong, rounded posteriorly (as in Fig. 1l or similar to nanulus group, for example, Fig. 1d). Elytral surface weakly punctate. Median lobe in lateral view as in Fig. 12b. New World	N. brevicornis Sharp, 1882
–	Body form ovoid, more elongate, more attenuated posteriorly (as in Fig. J). Elytral surface weakly to moderately punctate. Median lobe different. Indomalaya and Oceania	tenellus group
7	Protibia with robust seta of dorsal margin distinctly distad of half-length of outer margin, approximately at 2/3 margin length (Fig. 2e), distance between robust seta and dorsoapical angle distinctly shorter than distance between robust seta and first seta from protibial insertion. Dorsal coloration uniformly brown or bicolorous (Fig. 1d–f), with pronotum distinctly lighter than head and elytra. Elytral surface matte to somewhat shiny and iridescent	traili group
–	Protibia with robust seta of dorsal margin approximately at half-length of outer margin, distance between robust seta and dorsoapical angle subequal to distance between robust seta and protibial insertion (Fig. 2d). Color variable, but elytra of mature specimens of most species with darker triangle medially at base (Fig. 1g, i). Most species with elytral surface very shiny, iridescent	meizon group (in part)

Figure 1.

Notomicrus species groups. Dorsal habitus of representative Notomicrus species a N. nanulus b N. sharpi c N. sp. d N. cf. traili e N. cf. gracilipes f N. petrareptans g N. sp. h N. sp nr. malkini i N. sp. nr. meizon j N. tenellus k N. brevicornis male syntype.

Description of species groups

1. N. josiahi species group

Diagnosis

The josiahi group is diagnosed by the following combination of characters. Dorsal (outer) margin of protibia without notable robust seta at or near mid-length (Fig. 2a). Body form elongate, strongly, but regularly attenuated posteriorly. Eyes large relative to head capsule. Elytra with notable contrasting dark and light colors (Figs 5, 6); shiny and iridescent; microsculpture very fine. Prosternal process narrow (Fig. 3a). Protibiae elongate, with penultimate dorsal seta only slightly longer than others on dorsal margin (Fig. 2a); males with profemur lacking notable protuberance on posteroventral margin (Fig. 2a), only weakly angulate at mid-length; protarsal claws very small, distinctly less than half the length of protarsomere V (Figs 2a, 8, 9), not distinctly larger than female claws, anterior protarsal claw bifurcate, with small dorsal spur (Figs 8, 9). The large eyes, elytral color pattern and coloration and characters of the protibiae, make this species group easily distinguishable from others. There are only two species known.

Figure 2.

Representative prolegs of Notomicrus species groups (left proleg, anterior aspect) a josiahi group (N. josiahi) b nanulus group (N. nanulus) c meizon group (Notomicrus sp.) d meizon group, alternative setal spacing of dorsal (outer) protibial margin (Notomicrus sp.) e traili group (N. cf. traili) f detail of structures of importance. F1Se = setae of anteroventral margin of profemur; F1Sp = protuberance of posteroventral margin of profemur; PtCA = anterior protarsal claw; Ptm-5 = protarsomere V; SeDA = first robust seta of dorsoapical angle; SeMl = robust seta at mid-length of anterodorsal margin of protibia; SeM = First seta of anterodorsal margin of protibia; arrows indicate points for relative lengths (see key): i = anteroapical angle, ii = robust seta at mid-length, iii = first seta of marginal row.

Figure 3.

Representative prosterna of Notomicrus species groups a N. josiahi group (N. josiahi) b N. nanulus group (N. nanulus) c N. meizon group (N. sp. nr. malkini) d N. traili group (N. cf. traili).

Notomicrus josiahi Miller, 2013

Figs 2a, 3a, 4a, 5a, b, 6, 8

Notomicrus josiahi Miller, 2013: 244; Holotype: MIZA

Type locality

Venezuela, Amazonas State, Communidad Caño Gato, Rio Sipapo, 4°58.838'N, 67°44.341'W.

Material examined

Paratypes : “VENEZUELA: Amazonas State/ 4°58.845'N, 67°44.341'W, 100 m/ Communidad Caño Gato on Rio/ Sipapo; sandy stream; 7.i.2006; AS-06-016; leg. A.E.Z. Short” [White label, typed print] (1 female ex. SEMC); “VENEZUELA: Amazonas State/ 4°58.845'N, 67°44.341'W, 100 m/ Communidad Caño Gato on Rio/ Sipapo; 16.i.2009; leg. Short,/ Miller, Camacho, Joly, & Garcia/ VZ09-0116-01X; along stream” [White label, typed print] (1 male, 2 females ex. SEMC) All paratypes with white barcode label with the following numbers and “KUNHM-ENT”: “SM0843570” “SM0831496” “SM0842848” “SM0843672”; all paratypes with “PARATYPE/ Notomicrus josiahi/ Miller, 2013” [Blue label with black border, typed print].

Other material

Venezuela: Amazonas State, 4°58.845'N, 67°44.341'W, 100 m, Communidad Caño Gato on Rio Sipapo; 16.i.2009; leg. Short, Miller, Camacho, Joly, & Garcia/ VZ09-0116-01X; along stream (64 males and females ex. SEMC).

Measurements

TL = 1.46–1.69 mm (mean = 1.59 mm, SD. = 0.058, males = 1.46–1.69 mm, male mean = 1.58, SD. = 0.069, females = 1.55–1.68 mm, female mean = 1.62, SD. = 0.036); TLPn = 1.33–1.53 mm (mean = 1.44, SD. = 0.045, males = 1.33–1.49 mm, females = 1.43–1.53 mm); GW = 0.68–0.78 mm (mean = 0.74 mm, St. Dev. = 0.025, males = 0.68–0.78 mm, females = 0.72–0.78 mm); HW = 0.40–0.45 mm (mean = 0.42 mm, SD. = 0.014, males = 0.40–0.43 mm, females = 0.42–0.45 mm); EW = 0.16–0.19 mm (mean = 0.175 mm, SD. = 0.01, males = 0.16–0.17 mm, females = 0.17–0.19 mm); TL/GW = 1.99–2.31 (mean = 2.16; SD = 0.070; males = 1.99–2.31, females = 2.13–2.22); HW/EW = 2.21–2.53 (mean = 2.39, SD = 0.083, males = 2.41–2.53, females = 2.21–2.44).

Figure 4.

Representative noterid platforms of Notomicrus species groups (left side, ventral aspect). Names in boxes indicate species groups or species a N. josiahi b N. nanulus c N. sharpi d N. sp. (nr. chailliei) e N. sp. nr. meizon f N. meizon (paratype) g N. sp. nr. malkini h N. brevicornis (female syntype) i N. teramnus (female paratype) j N. tenellus (Indonesia) k N. sabrouxi (female paratype, sketched from Manuel (2015:518) l N. petrareptans.

Figure 5.

Notomicrus josiahi species group, dorsal and ventral habitus a, b Notomicrus josiahi Miller, 2013 (paratype) c, d Notomicrus interstinctus sp. nov. (paratype).

Diagnosis

Notomicrus josiahi can be diagnosed by the following combination of characters: (1) Size large TL = 1.46–1.69 mm; (2) elytron with strongly darkened region in anterior 1/3^rd, contrasting against brownish-yellow of rest of elytron (Fig. 5a); (3) Eyes very large relative to head capsule (HW/EW = 2.21–2.53; males 2.41–2.53, females 2.21–2.37); (4) aedeagus as in Fig. 6, median lobe expanded on right side in dorsal or ventral aspect, weakly attenuated to apex from mid-length in lateral aspect, with apex curved dorsolaterally to the left, left lateral lobe with dense tuft of setae at apex, few setae along dorsal margin and sparse tuft near base; right lateral lobe with small tuft of setae at apex; (5) pro- and mesotarsal claws as in Fig. 8, anterior protarsal claw strongly bent, bifurcate, with slender spur originating on dorsal margin where curved (Fig. 8a), ventral margin strongly expanded ventrally near base.

Figures 6, 7.

Aedeagi of josiahi species group 6 N. josiahi 7 Notomicrus interstinctus sp. nov. a median lobe, left lateral aspect b median lobe, dorsal aspect c median lobe, right lateral aspect d left lateral lobe, medial surface/aspect e right lateral lobe, medial surface/aspect. Scale bars: 100 µm

Figures 8, 9.

Pro- and mesotarsal claws of josiahi species group 8 N. josiahi 9 N. interstinctus sp. nov. a anterior protarsal claw b posterior protarsal claw c anterior mesotarsal claw d posterior mesotarsal claw. All anterior aspect. Scale bars: 25 µm.

Re-description

Males. Body elongate-oval, attenuated posteriorly (Fig. 5a), TL/GW = 1.99–2.31 lateral outline of elytra and pronotum continuous in dorsal aspect; regularly curved to head, posteriorly evenly attenuated to elytral apex from point of greatest width; widest point just posterior to humeral angles of elytra, as in Fig. 5a.

Color. Head, pronotum, venter and legs yellow; elytron dark brown to black in basal 1/3, darkened region extending posteriorly along elytral suture and contrasting against brownish-yellow color of posterior 2/3 of elytron (Fig. 5a); elytron with surface weakly iridescent. Venter and legs uniformly yellow (Fig. 5b).

Structures. Eyes very large relative to head capsule (HW/EW = 2.35–2.53); antennae with length greater than greatest width of head. Prosternal process narrow, not strongly constricted between procoxae, with apex attenuated (Fig. 3a). Noterid platform with lateral margins subparallel (weakly convergent in posterior 2/3, convergent in anterior 1/3 (Fig. 4a); posterior lobes acute, angled, acutely rounded at apex. Profemur with loose comb of 3–5 stiff setae on anteroventral margin (Fig. 2a), posteroventral margin weakly angled at mid-length (Fig. 2a). Protibia elongate, dorsal and ventral margins weakly divergent distally in anterior aspect (Fig. 2a), anterodorsal margin with row of 6–7 stout setae, without distinctly larger seta near mid-length. Protarsi with adhesive discs on ventral surface of protarsomeres II and III, lacking disc on ventral surface of protarsomere I; protarsal claws as in Fig. 8a, b, subequal in length, small, length ca. 1/3 that of protarsomere V, anterior claw distinctly bifurcate in distal half, expanded basally, very sharply curved, posterior claw slender, weakly expanded basally, moderately curved. Mesotarsi with adhesive discs on ventral surface of protarsomere II only, lacking disc on ventral surface of protarsomere I; mesotarsal claws as in Fig. 8c, d, subequal in length, small, length slightly greater than that of protarsal claws, slender, weakly expanded at base and weakly curved.

Sculpture. Dorsal surface of head with microsculpture very weakly impressed, microreticulation very fine, meshes mostly indistinct; micropunctation nearly indistinct. Pronotum with microsculpture similar to that of head, microreticulation fine; with scattered punctation near base and lateral margin, lateral punctures moderately dense, some with very fine setae. Elytron with microsculpture weakly impressed, microreticulation very fine, nearly indistinct; with punctation sparse in anterior half, with fine punctures along lateral margin and along discal row, with very few to no punctures between discal row and elytral suture, punctate in posterior half, punctures fine, many with very fine setae; discal row composed of fine and irregularly scattered punctures, denser posteriorly, lateral row similar to discal row but more sparse; micropunctation present, evenly scattered. Noterid platform and metaventrite surface with microsculpture weakly to moderately impressed, very fine, meshes of microreticulation nearly indistinct, cells transversely elongated.

Aedeagus. Aedeagus as in Fig. 6. Median lobe in lateral aspect gradually curved from base to apex, dorsal and ventral margins subparallel, converging at apex; apex acute, sharply curved, in ventral aspect subapically expanded and curved to left (Fig. 4a–c). Left lateral lobe in lateral aspect elongate, curved dorsally, with dense tuft of setae at apex (Fig. 6d). Right lateral lobe in lateral aspect oval; apex rounded with small tuft of setae in apical cleft (Fig. 6e).

Females. As males, except eyes slightly smaller than in males (HW/EW females = 2.21–2.39); profemur with posteroventral margin smooth, lacking weak angle at mid-length; pro and mesotarsomeres unmodified, slender, lacking adhesive discs; pro and mesotarsal claws unmodified, claws of respective tarsi subequal in length, slender, weakly curved.

Variation

As this species is known from only a single series, it is difficult to assess the degree of intraspecific variation. However, some variation was observed in the relative lightness or darkness in coloration of the individuals, with some brighter in color, more yellow, and others darker in color, more brownish yellow. The darkened region of the elytra also varied somewhat, occupying 1/4 to greater than 1/3 of the basal region of the elytron.

Differential diagnosis

Notomicrus josiahi is among the most easily distinguished species of Notomicrus by the combination of the large eyes, color pattern, shape of male protarsal claws and of male aedeagus. Superficially, N. josiahi is similar to some species of the N. meizon group in color, wherein N. meizon Guimarães & Ferreira-Jr, 2019, N. malkini Young, 1978 and other undescribed species are also darkened at the base of the elytra. However, in N. josiahi, this darkened area is better defined with the posterior border less oblique, thus expanding more completely over the humeral angles of the elytron. More distinctly, N. josiahi differs from these and other species by the much larger eyes and bifurcate protarsal claws (in males), which to date, has only been observed in N. interstinctus, N. brevicornis and the tenellus group. Among all other species of Notomicrus, the aedeagus of N. josiahi is distinct, with the right lateral lobe rounded and bearing a small tuft of setae at apex, rather than without setae, as in all other neotropical species.

Distribution

Known only from Venezuela (Fig. 10).

Figure 10.

Distribution map of josiahi group species.

Ecology

This species has been collected from only a single locality, from the margins of a small, sandy stream (Fig. 11a).

Figure 11.

Habitats of N. josiahi group species a Type locality of N. josiahi, Venezuela: Amazonas (collection code VZ09-0116-01A); Localities of N. interstinctus b Brazil, Amazonas (collection code BR17-0609-01A) c type locality, Brazil, Amapá (collection code BR18-0722-01A).

Notomicrus interstinctus sp. nov.

http://zoobank.org/9098C43C-66D4-4245-8F3F-5A4F7FB62549

Figs 5c, d, 7, 9

Type locality

Brazil: Amapá, Calcoene, 2.50019, -50.97712.

Material examined

Holotype, male: “BRAZIL: Amapá: Calcoene/ 2.50019°, -50.97712°; 5 m/ Colcoene (1 km W) on BR-156/ 22.vii.2018; leg. Short; Marshy/ savannah; BR18-0722-01A” [White label, typed print] “HOLOTYPE/ Notomicrus/ interstinctus/ Baca & Short, 2020” [Red label, black border, typed print] (ex.INPA). Paratypes: Same data as holotype, except with “PARATYPE/ Notomicrus/ interstinctus/ Baca & Short, 2021” [Blue label, black borders, typed print] (4 males, 5 females exs. SEMC, INPA); Brazil: Amazonas, Manacapuru Municipality, -3.23037, -60.64269, 35 m, 9.vi.2017, leg. Benetti, margin of large marsh/river, lots of vegetation; BR17-0609-01A; with “PARATYPE/ Notomicrus/ interstinctus/ Baca & Short, 2021” [Blue label, black borders, typed print] (3 males, 6 females exs. SEMC, INPA).

Measurements

TL = 1.50–1.63 (Holotype = 1.50 mm, mean = 1.56 mm, SD. = 0.045, males 1.50–1.63 mm, females 1.50–1.63 mm); TLPn = 1.38–1.48 (Holotype = 1.40 mm, mean = 1.42 mm, SD = 0.039, males = 1.40–1.45 mm, females = 1.38–1.48 mm); GW = 0.72–0.80 mm (Holotype = 0.72 mm, mean = 0.75 mm, SD. = 0.027, males = 0.72 mm–0.76 mm, females = 0.73–0.80 mm; HW = 0.41–0.45 mm (Holotype = 0.41 mm, mean = 0.43 mm, SD. = 0.013, males = 0.41–0.42 mm, females = 0.42–0.45 mm); EW = 0.18–0.22 mm (Holotype = 0.18 mm, mean = 0.20 mm, SD. = 0.013, males = 0.18–0.19 mm, females = 0.19–0.22 mm), TL/GW = 1.99–2.26 (Holotype = 2.08, mean = 2.07, SD. = 0.074, males = 2.06–2.26, females = 1.98–2.11); HW/EW = 2.04–2.33 (Holotype = 2.28, mean = 2.19, SD. = 0.088, males = 2.16–2.33, females = 2.04–2.26)

Diagnosis

Notomicrus interstinctus can be diagnosed by the following combination of characters: (1) Size large TL = 1.53–1.63 mm; (2) elytron dark with contrasting yellow band at mid-length, apices yellow (Fig. 5c, d); (3) eyes very large relative to head capsule (HW/EW = 2.04–2.33; Fig. 5c); (4) aedeagus as in Fig. 7; median lobe not broadly expanded on right side in dorsal aspect, attenuated to apex in lateral aspect, with small, round apical club oriented laterally to left; left lateral lobe with row of setae along entire dorsal margin, only somewhat denser at apex; right lateral lobe glabrous, without small tuft of setae at apex; (5) protarsal claws as in Fig. 9a, b; anterior protarsal claw strongly bent, bifurcate, branching at mid-length, ventral margin somewhat expanded ventrally near base.

Description

Holotype. As described for N. josiahi, except the following. Size large, TL = 1.53 mm. Body very broad, elongate-oval, strongly attenuated posteriorly, TL/GW = 2.08; lateral outline of elytron evenly and gradually curved to apex from point of greatest width, as in Fig. 5c, d.

Color. Dorsal surface of head brown, lighter near clypeus; pronotum yellow; elytron dark, nearly black in anterior and posterior thirds, with lighter contrasting brownish-yellow transverse band near mid-length of elytron, elytral apex also lighter, brownish-yellow; elytron with surface moderately iridescent. Ventral surface of head and prosternum light brownish-yellow; rest of venter yellowish-brown; legs yellow.

Structures. Eyes large relative to head capsule (HW/EW = 2.28). Posterior lobes of noterid platform with angles acute, apices rounded (as in Figs 3a, 5d). Pro- and mesotarsal claws as in Fig. 9.

Sculpture. Elytron with punctation as described in N. josiahi, but denser overall and less restricted to posterior half, with punctures along lateral margin and puncture rows more widely distributed and denser.

Aedeagus. Aedeagus as in Fig. 7. Median lobe in lateral aspect, strongly curved at base, distally weakly curved, nearly straight; dorsal and ventral margins subparallel to mid-length, then attenuated to apex; apex with small club, sharply bent dorsally and left; left lateral lobe in lateral aspect, elongate, dorsal margin curved with dense row of fine setae (Fig. 7d). Right lateral lobe in lateral aspect oblong, rounded distally.

Females. As males, except eyes slightly smaller (HW/EW = 2.04–2.16); profemur with posteroventral margin smooth, lacking weak angle at mid-length; pro- and mesotarsomeres unmodified, slender, lacking adhesive discs; pro- and mesotarsal claws unmodified, claws of respective tarsi subequal in length, slender, weakly curved.

Variation

The most notable variation was in size and color, with some specimens darker overall than others, with the yellow bands sometimes smaller.

Differential diagnosis

Notomicrus interstinctus is easily distinguished by the combination of large eyes and elytral color pattern, darkened in anterior and posterior thirds with a yellow transverse band. This color pattern is unique among known species of Notomicrus. This species is also unusual in that it is one of the few known species (along with N. brevicornis, N. josiahi and members of the tenellus group), with males that present bifurcated anterior protarsal claws. The aedeagus, color pattern and more subtly the denser punctation, easily differentiates this species from N. josiahi. The aedeagus of N. interstinctus is similar to that of the N. traili group with the median lobe attenuated and the apex enlarged and bent in a left-dorsal direction, but other external characters, such as the color pattern, tarsal claws and large eyes, readily distinguish this species from the traili group. The elytral punctuation is somewhat similar to that of some members of the N. meizon group, being somewhat densely punctate posteriorly, with punctures fine, but the aforementioned combination of characters will differentiate N. interstinctus from these species as well.

Etymology

Notomicrus interstinctus sp. nov. derives its name from the Latin adjective interstinctus, meaning checkered or variegated. This refers to the color pattern of the elytra of this species. It is treated as an adjective in the nominative singular.

Distribution

Known from northern Brazil, Amazonas and Amapá states (Fig. 10).

Ecology

The species seems to be a generalist in terms of habitat, but seems to prefer vegetated environments. It was collected from a very shallow open marshy area (Fig. 11c) in the Brazilian state of Amapá and the vegetated margins of a river in Amazonas (Fig. 11b).

Taxonomic comments

Notomicrus interstinctus appears very similar to specimens misidentified as N. traili Sharp, 1882 by Guimarães and Ferreira-Jr (2019). This was due to the similarities of the size, punctation and shape of the aedeagus. The records from this work would potentially add to the distribution above, as most appear to be from the same regions of the Amazon Basin as the Amazonas specimens. Verification will be needed to confirm these individual records and these are not formally attributed to N. interstinctus here. Observations of the lone female syntype of N. traili (NHM) indicate that the species is as described by Young (1978), with males attributable to N. traili, appearing as in Fig. 2, with the head and elytra brown, without a pattern.

2. Notomicrus nanulus species group

Diagnosis

Members of this species group are most easily identified by their (1) monotone brown elytral color (Fig. 1a–c); (2) rounded, oval body shape; (3) rounded posterior lobes of the noterid platform (Fig. 4b–d); and (4) by their coarser microsculpture, consisting of isodiametric cells, appearing scale-like, rather than as a finer mesh of transversally elongated cells. This latter character is best viewed in light reflecting off the elytra. Even in species with finer variants of the cell-like microsculpture (e.g. N. sharpi), there is no iridescence. Punctation is largely indistinct, except for the discal series and sometimes sporadic punctures posteriorly.

Members of the N. nanulus group present a combination of characters that are variably shared with N. brevicornis, N. teramnus and the members N. tenellus group. This pattern, in tandem with our phylogenetic understanding, for example, tenellus group being sister to all other Notomicrus (Baca and Short 2020), suggest that N. nanulus species are united by a combination of characters that are plesiomorphic at some level within the genus. All share similar microsculpture consisting of isodiametric cells, often appearing scale-like to some degree. However, the N. nanulus group is distinguished from the N. tenellus group by the more rounded body outline (Fig. 1a–c, j) and from the N. tenellus group and N brevicornis by the shape of the noterid platform, with the nanulus group presenting posterior lobes that are rounded or squared (Fig. 4b–d). The rounded/squared lobes of the noterid platform character distinguish the nanulus group from N. teramnus (Fig. 4i) also, but more subtly. The nanulus group also typically presents a noterid platform with more longitudinally-elongated proportions than N. brevicornis (Fig. 4h). Males of the N. nanulus group present unbifurcated anterior protarsal claws, unlike N. brevicornis and the N. tenellus group. The aedeagi of known males of the N. nanulus group are easily distinguished; see Young (1978) and Manuel (2015).

Composition

N. chailliei Manuel, 2015; N. femineus Manuel, 2015; N. huttoni Young, 1978; N. nanulus (LeConte, 1863); N. sharpi Balfour-Browne, 1939.

Identification resources

Young (1978); Manuel (2015).

Remarks

Future work on this group may prove difficult as many species are collected with high ratios of females to males. An example was N. femineus Manuel, 2015, in which extensive collecting yielded only females, raising the possibility of parthenogenetic reproduction. Personal observations indicate that multiple undescribed species are represented by females only. We note that females of this group can be especially difficult to distinguish and are often misidentified as N. brevicornis (see comments on N. brevicornis, below). Notomicrus teramnus is a potential member of this species group based on color, shape and microsculpture, but is treated separately in the key, pending further investigation (see insertae sedis species below).

3. Notomicrus meizon species group

Diagnosis

Non-teneral specimens of this group tend to have the following combination of characters: (1) triangular pigmented area medially on the base of the elytra (Fig. 1g–i), similar to N. josiahi, but not as prominent and not always discernible in some populations or in teneral specimens; (2) dense, fine punctures bearing short setae on the posterior half of the elytra and sometimes extending far anteriorly (not as coarse as in members of the traili group); (3) microreticulation variably impressed, consisting of fine mesh-like reticulation; often iridescent; (4) posterior lobes of noterid platform with squared or rounded angles (Fig. 4e); if posterior angles of noterid platform more acute (Fig. 4f, g), protibia presents robust seta of outer margin approximately at half-length of outer margin, distance between robust seta and dorsoapical angle subequal to distance between robust seta and first seta from protibial insertion (Fig. 2d).

Composition

N. malkini Young, 1978; N. meizon Guimarães & Ferreira-Jr, 2019.

Identification resources

Young (1978); Guimarães and Ferreira-Jr (2019).

Remarks

The meizon group is sometimes difficult to discern from the traili group, as the differences amongst diagnostic characters can be subtle. The darkened basal area of the elytra in the meizon group is helpful, but investigators may find great difficulty in diagnosing teneral members of this group, which often lack the pigmented triangular area on the elytra. It is important to note that this darkened area is truly pigmented, not just darker in appearance due to the folding of the wings under the elytra as often happens in lighter colored species (as in Fig. 1f, j, k). Fortunately, males of most individual species of the meizon group are easy to identify by their aedeagi in combination with other characters, such as tarsal claws. The male median lobes of the meizon group species are usually (but not always) very irregularly shaped (for example, see Young 1978 and Guimarães and Ferreira-Jr 2019). The aedeagus of most species of the traili group appear similar to Fig. 8, with a small club at apex, often hooked to the left (see Young 1978; Manuel 2015; Baca and Short 2018). Additionally, males of meizon species often present notable unequal lengths between the anterior and posterior protarsal claws. These are usually subequal in length in the traili group.

4. Notomicrus traili species group

Diagnosis

Non-teneral specimens of this group tend to have the following combination of characters: (1) lacking triangular pigmented area on the medial base of the elytra (Fig. 1d–f), lacking maculae; elytra with uniform shades of tan or brown; (2) irregular setose punctures in posterior half of elytral surface with density variable, increasingly coarse if extending on to anterior half of elytron; (3) microreticulation variably impressed, consisting of fine mesh-like reticulation; matte to shiny; elytral surface sometimes somewhat iridescent; (4) posterior lobes of noterid platform with angles acute (Fig. 4k, l); (5) protibia as in Fig. 2e, with robust seta of outer margin at ca. 2/3 length of margin, distance between robust seta and dorsoapical angle distinctly less than distance between robust seta and first seta from protibial insertion.

Composition

N. gracilipes Sharp, 1882; N. petrareptans Baca & Short, 2018; N. reticulatus Sharp, 1882; N. sabrouxi Manuel, 2015; N. traili Sharp, 1882.

Identification resources

Young (1978); Manuel (2015); Baca and Short (2018).

Remarks

Species of the traili group are difficult to discern and constitute a widespread species complex (see Baca and Short 2020). Personal observations coupled with the phylogenetic reconstructions of Baca and Short (2020) show that the diagnostic power of the dorsal punctation (see Young 1978) is unreliable, with multiple clades within the complex sharing similar patterns of punctation; for example, the pattern of punctation attributed to N. gracilipes by Young (1978) arises in multiple places within the complex. The group will require careful taxonomic investigation. The members of the traili group can be difficult to distinguish from those of the meizon group, but mature members lack a pigmented triangular area at the base of the elytra and most males of the traili group have similarly-shaped median lobes of the aedeagus, distinct from the meizon group. See notes in remarks of meizon group above.

5. Insertae sedis species

These species present characters combinations not found in other species groups. Both by presented character combination and even general gestalt, these are difficult to place with certainty. Molecular sequence data were unavailable for these species in the phylogenetic reconstruction of Baca and Short (2020). In particular, the species listed here both exhibit body shape, color, microsculpture and sparse punctation that would place them in the N. nanulus species group. However, in comparison with the N. tenellus species group, the sister to all New World taxa, several of these characters appear plesiomorphic in Notomicrus, making it difficult to discern their likely relatives from morphology alone.

N. brevicornis Sharp, 1882

Figs 1k, 12

Material examined

Syntypes : Male specimen on small rectangular card, “♂” is drawn around genitalia and other parts, prosternal process flanks the specimen. “Boa Sorta Nov./ Sahlberg 1850” [small rectangular label, handwritten], “Sharp Coll/ 1905-313” [small rectangular label, typed], “Notomicrus/ brevicornis Ind. typ./ D.S.” [small rectangular label, handwritten] “SYN/ TYPE” [small circular label with blue border, printed] (ex. NHM); female specimen on rectangular card, “S. America/ Brazil.” [small rectangular label with blue line across, typed], “Sharp Coll/ 1905-313.” Small rectangular label, typed], “Boa Sorta Nov./ Sahlberg 1850” [small rectangular label, handwritten], “Type 470/ Notomicrus/ brevicornis/ Boa Sorta” [rectangular label, handwritten], “SYN/ TYPE” [small circular label with blue border, printed], “TYPE” [small circular label with red border, printed], (ex. NHM); female specimen disarticulated on large card, “S. America/ Brazil.” [small rectangular label with blue line across, typed], “Boa Sorta Nov./ Sahlberg 1850” [small rectangular label, handwritten], “Notomicrus/ brevicornis, Sharp./ Co-type.” [rectangular label, handwritten], “SYN/ TYPE” [small circular label with blue border, printed], (ex. NHM); female specimen on small rectangular card, “Co-/ type” [small circular label with yellow border, printed], “S. America/ Brazil.” [small rectangular label with blue line across, typed], “Sharp Coll/ 1905-313.” Small rectangular label, typed], “Notomicrus/ brevicornis, Sharp./ Co-type.” [rectangular label, handwritten] “SYN/ TYPE” [small circular label with blue border, printed] (ex. NHM). Note: this latter specimen also with small label “Not brevicornis/ maybe gracilipes?/ Manuel det. 2016”. See notes below.

Remarks

Notomicrus brevicornis would otherwise appear to be a member of the nanulus group by the aforementioned characters. However, it differs by the more acute posterior angles of the noterid platform, a character shared with members of the tenellus, josiahi and traili groups. The male syntype presents a bifurcate anterior protarsal claw (as in fig. 8A), a character shared by the josiahi and tenellus species groups. With the Old World and New World taxa being reciprocally monophyletic (Baca and Short 2020) and the plesiomorphic appearance of these characters, we would speculate that this species is likely to be sister to one of the New World species groups.

Based on observation of the single male of the syntype series, it is suspected that Young (1978) based his description, key and illustration of the aedeagus of N. brevicornis on the male of a different species. First, the illustration in Young (1978) of the aedeagus of N. brevicornis does not match that observed in the syntype. Second, Young (1978: 288–289) describes N. brevicornis as being sexually dimorphic in elytral punctation, with males being more punctate than females. However, as noted by Sharp (1882: 261), there is very little dimorphism observed between males and females of the syntype series beyond characters of the tarsi. The punctation and sculpture are very weakly dimorphic, both sexes being almost entirely impunctate, except for the weak discal rows and a few scattered punctures near the elytral apex. The punctation is slightly less impressed in females, with discal rows slightly less prominent. The relative difference of punctation between the male and female syntypes of this species is so slight that splitting them up in the key as did Young (1978: 288, couplet 7) seems largely unnecessary, wherein the couplet describing females of N. brevicornis also closely describes the male syntype (Young 1978: 288). The specimens of the UMMZ, observed by Young, were not observed for this study, but the stated differences by Young (1978) and the grouping of males of N. brevicornis with N. malkini in Young’s (1978: 288) key call the identity of the depicted male in Young (1978) into question. Further adding to this suspicion is the fact that some male specimens attributable to N. malkini or other undescribed species of the meizon group in the FSCA were identified as N. brevicornis by Young (date of determination not recorded). For aiding in identification, we have included images of the male syntype, labels and aedeagus (Figs 1k, 12). One specimen of the syntype series appears to be of a different species than the others; likely it is a member of the traili species group. See last listed specimen and note in the examined syntype material above.

Figure 12.

Card mount, aedeagus and labels of male syntype of N. brevicornis a Notomicrus brevicornis card mount, dorsal b median lobe lateral aspect c left lateral lobe, medial aspect d right lateral lobe e syntype labels.

Personal observations show that many members of the N. nanulus group are misidentified as N. brevicornis in collections. This is no doubt due to the superficial similarities of N. brevicornis to members of the nanulus group and scarcity of males in the nanulus group. With that, there are likely inaccuracies in literature with respect to records and distributions.

N. teramnus Guimarães & Ferreira-Jr, 2019

Remarks

Notomicrus teramnus would also appear a member of the nanulus group, given the above-mentioned characters. An argument could be made that this is the case as it only appears to differ in the shape of the posterior lobes of the noterid platform being more angular than most species in the nanulus group. This species otherwise appears to lack characters that would unite it with other species groups, though this will require examination and/or phylogenetic investigation. We abstain from placing it as member of the nanulus group as N. teramnus is known only from a high elevation hygropetric habitat, which may present confounding morphological specialization. Aedeagal morphology is not here considered to be indicative of a particular placement, but the very unusual morphology of the aedeagus of N. teramnus (see Guimarães and Ferreira-Jr 2019) further raises questions of placement.

Acknowledgements

We are grateful for the assistance and support of many colleagues during fieldwork in Brazil, including Neusa Hamada (INPA) and Cesar Benetti (INPA). We would also like to extend a warm thanks to our colleagues M. Manuel, M. Toledo and B. Guimarães for helpful comments, specimens and information over the course of this ongoing project and a special thanks to the reviewers of the manuscript for their consideration and constructive comments. This study was supported in part by US National Science Foundation grant DEB-1453452 to AEZS and US National Science Foundation NSF GRF #0064451 to SMB. Fieldwork in Brazil was partly funded by a Fulbright fellowship to AEZS and conducted under SISBIO license 59961-1.

References

Baca SM, Short AEZ (2018) Notomicrus petrareptans sp. n., a new seep-dwelling species of Noteridae from Suriname (Coleoptera: Adephaga). Zootaxa 4388(2): 182–190. https://doi.org/10.11646/zootaxa.4388.2.2

Baca SM, Short AEZ (2020) Molecular phylogeny of the notomicrine water beetles (Coleoptera: Noteridae) reveals signatures of Gondwanan vicariance and ecological plasticity. Insect Systematics and Diversity 4(6): 1–4. https://doi.org/10.1093/isd/ixaa015

Baca SM, Toussaint EFA, Miller KB, Short AEZ (2017) Molecular phylogeny of the aquatic beetle family Noteridae (Coleoptera: Adephaga) with an emphasis on partitioning strategies. Molecular Phylogenetics and Evolution 107: 282–292. https://doi.org/10.1016/j.ympev.2016.10.016

Balfour-Browne J (1939) A contribution to the study of the Dytiscidae. – I. (Coleoptera, Adephaga). The Annals and Magazine of Natural History (11) 3: 97–114. https://doi.org/10.1080/00222933908526903

Belkaceme T (1991) Skelet und muskulatur des kopfes und thorax von Noterus laevis Sturm.ein beitrag zur morphologie und phylogenie der Noteridae (Coleoptera: Adephaga). Stuttgarter Beiträge zur Naturkunde A 462: 1–94.

Beutel RG, Roughley RE (1987) On the systematic position of the genus Notomicrus Sharp (Hydradephaga, Coleoptera). Canadian Journal of Zoology 65(8): 1898–1905. https://doi.org/10.1139/z87-290

Clark H (1863) Descriptions of new East-Asiatic species of Haliplidae and Hydroporidae. T. Roy. Entomological Society of London (3) 1: 417–428. https://doi.org/10.1111/j.1365-2311.1862.tb01288.x

Fauvel A (1903) Faune analytique des coléoptères de la Nouvelle-Calédonie. Revue d’Entomologie 22: 203–379.

Guimarães BA, Ferreira-Jr N (2019) Two new species and new records of Notomicrus Sharp, 1882 (Coleoptera: Noteridae) from Brazil. Zootaxa 4629(2): 263–270. https://doi.org/10.11646/zootaxa.4629.2.8

LeConte JL (1863) New species of North American Coleoptera. Part 1. Smithsonian Miscellaneous Collections 6(167): 1–92.

Manuel M (2015) The genus Notomicrus in Guadeloupe, with description of three new species (Coleoptera: Noteridae). Zootaxa 4018(4): 506–534. https://doi.org/10.11646/zootaxa.4018.4.2

Miller KB (2009) On the systematics of Noteridae (Coleoptera: Adephaga: Hydradephaga): phylogeny, description of a new tribe, genus and species, and survey of female genital morphology. Systematics and Biodiversity 7: 191–214. https://doi.org/10.1017/S1477200008002946

Miller KB (2013) Notomicrus josiahi, a new species of Noteridae (Coleoptera) from Venezuela. Zootaxa 3609(2): 243–247. https://doi.org/10.11646/zootaxa.3609.2.11

Miller KB, Nilsson AN (2003) Homology and terminology: Communicating information about rotated structures in water beetles. Latissimus 17: 1–4.

Nilsson AN (2011) A World Catalogue of the family Noteridae, or the Burrowing water beetles (Coleoptera: Adephaga). Version 16.VIII.2011, 54 pp. http://www2.emg.umu.se/projects/biginst/andersn/WCN/WCN_20110816.pdf [accessed 1 June 2017]

Sharp D (1882) On aquatic carnivorous Coleoptera or Dytiscidae. Scientific Transactions of the Royal Dublin Society 2: 179–1003. https://www.biodiversitylibrary.org/page/10137421

Toledo M (2010) Noteridae: Review of the species occurring east of the Wallace line (Coleoptera). In: Jäch MA, Balke M (Eds) Water Beetles of New Caledonia (part 1): monographs of Coleoptera (Vo. 3). Zoologische-Botanische Gesellshaft, Vienna, 195–236.

Young FN (1978) The New World species of the water-beetle genus Notomicrus (Noteridae). Systematic Entomology 3(3): 285–293. https://doi.org/10.1111/j.1365-3113.1978.tb00121.x