Research Article |
Corresponding author: Carlos Pedraza-Lara ( pedrazal@gmail.com ) Academic editor: Luis Ernesto Bezerra
© 2021 Carlos Pedraza-Lara, Pedro Joaquín Gutiérrez-Yurrita, Vladimir Salvador De Jesus-Bonilla.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Pedraza-Lara C, Gutiérrez-Yurrita PJ, Jesus-Bonilla VSD (2021) A new species of Procambarus (Decapoda, Cambaridae) from the State of Querétaro, Mexico. ZooKeys 1048: 1-21. https://doi.org/10.3897/zookeys.1048.57493
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With a Nearctic distribution, the family Cambaridae harbors a high species richness in Mexico, which is also evident along the Pánuco River catchment. A series of surveys carried on in five populations from the Sierra Gorda Biosphere Reserve in the State of Querétaro resulted in localizing a putative new species for science. A molecular phylogenetic study and species delimitation analyses including all the known Procambarus species from the Pánuco River catchment were conducted based on three mitochondrial genes (16S rDNA, 12S rDNA, and COI; 2,462 bp in total). Phylogeny recovered all species as monophyletic, including the populations under study. All delimitation results based on barcoding, ABGD, GMYC, bPTP, and gonopod differentiation agree in the recognition of a new taxon, to which the name Procambarus xihui sp. nov. is given, and its diagnosis and description are provided. The new species can be distinguished from the remaining species in the genus, among other characters, by a unique configuration of the terminal elements of the first pleopod of form I male, which includes a central projection lamellate, hood-like, forming a concave blade-like structure mesially directed, as well as a caudal process crest-like, mesiodistally directed, forming a lateral side of the concavity.
Astacidea, Crayfish, integrative taxonomy, species delimitation, systematics
The genus Procambarus Ortmann, 1905 encompass 45 native species and subspecies occurring in Mexico, inhabiting both Atlantic and Pacific coasts. An important part of the species richness in Mexico inhabits the Pánuco water basin, along the Sierra Madre Oriental and north of the Trans Mexican Volcanic Belt. To date, seven species have been recorded from that region: Procambarus cuevachicae (Hobbs, 1941), P. hidalgoensis López-Mejía, Álvarez & Mejía-Ortíz, 2005, P. roberti Villalobos & Hobbs, 1974, P. strenthi Hobbs, 1977, P. toltecae Hobbs, 1943, P. villalobosi Hobbs, 1969, and P. xilitlae Hobbs & Grubbs, 1982. In a survey of the diversity of the genus, previous studies located a series of populations from the aforementioned basin, in the Sierra Gorda Biosphere Reserve, at the northern side of the State of Querétaro, whose specific identity could not be confirmed (
A series of collections were made in the Sierra Gorda Biosphere Reserve (SGBR) for 20 years, beginning in 2002 (Table
Species, locality data, and GenBank accession numbers of specimens used in the phylogenetic and species delimitation analyses.
Species | Locality | Specimen | Collection year | GenBank accession numbers | ||
---|---|---|---|---|---|---|
16S | 12S | cox1 | ||||
Procambarus xihui sp. nov. | Arroyo de Los Álamos, Yerbabuena, Jalpan de Serra, Querétaro * | CPLC1† | 2019 | MW280269 | MW280231 | MW266807 |
Arroyo de Los Álamos, Yerbabuena, Jalpan de Serra, Querétaro * | CPLC23‡ | 2019 | MW280277 | MW280238 | MW266814 | |
Arroyo Camelinas, Yerbabuena, Querétaro | CPLC27 | 2002 | MW280280 | MW280242 | MW266816 | |
San Juanito, Landa de Matamoros, Querétaro | CPLC24 | 2002 | – | MW280239 | – | |
Río Verdito, Landa de Matamoros, Querétaro | CPLC25 | 2019 | MW280278 | MW280240 | – | |
Saldiveña, Jalpan de Serra, Querétaro | CPLC26 | 2007 | MW280279 | MW280241 | MW266815 | |
P. toltecae | Stream 1 Km Soutwest of Palitla, San Luis Potosí* | CPLC3 | 2019 | MW280270 | MW280246 | MW266808 |
Stream 1 Km Soutwest of Palitla, San Luis Potosí* | CPLC28 | 2019 | MW280281 | MW280243 | MW266817 | |
Huichihuayán, San Luis Potosí** | PopHui | 2012 | JX127823 | JX127687 | JX127966 | |
P. hidalgoensis | Stream on driveway from Tlanchinol-Olotla, Hidalgo | CPLC5 | 2019 | MW280272 | MW280233 | MW266810 |
Stream on driveway from Tlanchinol-Olotla, Hidalgo | CPLC29 | 2019 | MW280282 | MW280244 | – | |
P. villalobosi | Cave East of Rayón, San Luis Potosí* | CPLC11 | 2019 | MW280274 | MW280235 | MW266812 |
P. villalobosi | Cave East of Rayón, San Luis Potosí* | CPLC33 | 2019 | MW280285 | – | MW266820 |
P. gonopodocristatus | María de la Torre, Veracruz* | CPLC30 | 2019 | MW280283 | – | MW266818 |
María de la Torre, Veracruz* | CPL2474 | 2019 | MW280268 | MW280230 | – | |
P. roberti | Creek coming from La Media Luna, 0.5 Km East, San Luis Potosí* | CPLC13 | 2019 | MW280276 | MW280237 | – |
P. roberti | Creek coming from La Media Luna, 0.5 Km East, San Luis Potosí* | CPLC32 | 2007 | MW280284 | MW280245 | MW266819 |
P. roberti | –*** | roberti1 | – | KX238070 | – | – |
P. strenthi | Santa Anita spring, San Luis Potosí* | CPLC10 | 2018 | MW280273 | MW280234 | MW266811 |
–*** | strenthi1 | 2017 | KX238078 | – | – | |
P. caballeroi | Stream southern of Xicotepec de Juárez, Puebla* | 2419 | 2019 | MW280265 | MW280226 | MW266803 |
Stream southern of Xicotepec de Juárez, Puebla* | 2420 | 2019 | MW280266 | MW280227 | MW266804 | |
–*** | Pcb302 | – | KX238005 | – | – | |
P. cuevachicae | La Cueva Chica, Ciudad Valles, San Luis Potosí* | 2424 | 2020 | – | MW280228 | MW266805 |
La Cueva Chica, Ciudad Valles, San Luis Potosí* | 2425 | 2020 | MW280267 | MW280229 | MW266806 | |
P. acutus | Canal en Ciudad Mante, Tamaulipas | 3952 | 2007 | MW280264 | – | MW266802 |
Canal en Ciudad Mante, Tamaulipas** | PopMan | 2007 | JX127827 | – | JX127970 | |
P. digueti | Camécuaro River, Michoacán | CPLC12 | 2012 | MW280275 | MW280236 | MW266813 |
P. regiomontanus | Guadalupe, Nuevo León | CPLC4 | 2018 | MW280271 | MW280232 | MW266809 |
*** | DJ43 | 2018 | KX238068 | KX238138 | KX238224 |
Measurements of types. Morphometric measurements (mm) of holotype, allotype, and morphotype of P. xihui sp. nov.
Measurements | Holotype | Allotype | Morphotype |
---|---|---|---|
Total Length (TL) | 59.80 | 61.41 | 65.68 |
Cephalothorax | |||
Length (CL) | 28.89 | 28.99 | 32.15 |
Height (CH) | 13.91 | 14.11 | 15.63 |
Width (CW) | 13.71 | 13.99 | 15.26 |
Cephalon length (CEL) | 18.95 | 19.75 | 21.29 |
Abdomen width (AW) | 12.08 | 11.82 | 13.21 |
Rostrum | |||
Length (RL) | 7.26 | 7.18 | 8.60 |
Width (RW) | 4.85 | 5.59 | 5.65 |
Acumen length (AL) | 1.39 | 1.03 | 1.77 |
Antennal scale length (ASL) | 6.11 | 6.41 | 6.86 |
Cheliped | |||
Chela length (CHL) | 25.75 | 19.22 | 27.82 |
Chela width (CHW) | 8.02 | 5.98 | 7.84 |
Dactyl length (DL) | 14.50 | 11.30 | 16.01 |
Palm length (PL) | 9.56 | 7.12 | 8.81 |
Merus length (ML) | 13.13 | 11.38 | 13.32 |
Areola | |||
Areola width (ARW) | 9.12 | 8.79 | 10.56 |
Areola length (ARL) | 2.00 | 2.05 | 2.51 |
Aiming to account for an accurate representation of Mexican species of Procambarus, except for the troglobitic Procambarus xilitlae, all the species previously assigned to the subgenus Ortmannicus (
Specimens were identified using the appropriate taxonomic keys (
Specimens were preserved in ethanol and a piece of abdominal muscle was taken for DNA purification, which was carried on using a phenol-chloroform protocol (
Variability parameters of analyzed gene fragments and the most accurate substitution models.
Gene | Primers* | bp | V | PI | Model** |
---|---|---|---|---|---|
16S | 1471 | 559 | 169 | 82 | GTR+G |
16S-1472 | |||||
12S | 12sf | 397 | 71 | 51 | GTR+I+G |
12sr | |||||
COI | ORCO1F | 1506 | 221 | 138 | GTR+I+G |
ORCO1R |
To investigate the species limits between the putatively undescribed taxon and other Procambarus species with molecular information we used the following approaches: genetic divergence of the barcoding COI gene (
The uncorrected P-distances and standard error of the COI marker between putative species were calculated in Mega 10.1.8, estimating standard error based on bootstrapping (
For the GMYC approach, an ultrametric tree was reconstructed in Beast 2.6.2 (
A phylogenetic hypothesis regarding the included specimens of Procambarus species was reconstructed with Maximum Likelihood (ML) and Bayesian Inference (BI) methods. These analyses were carried out to evaluate the congruence of the delimitation analyses previously mentioned with the formation of monophyletic clades at the terminals and evaluate its clade support. Conformation to monophylly is also another way to assist during taxon recognition (
It has been described that habitats in the SGBR face important threats like increasing drying (
The data underpinning the analysis reported in this paper are deposited at GBIF, the Global Biodiversity Information Facility, and are available at https://doi.org/10.15468/3hu4bh.
In all cases, morphological features were congruent and stable when several form I male specimens were available for one species. No issues were evident when separating and identifying species according to the literature. As usual in Procambarus, the morphology of the first pair of pleopods of male form I was useful to identify and distinguish the new species, as the structure of terminal elements was always congruent with what was originally described and allowed robust species identification. Accordingly, a series of unique traits were observed for the populations from the SGBR.
The following gene fragments were obtained: 16S (559 bps), 12S (397 bps), and COI (1506 bps), resulting in 2462 characters and giving a series of substitution models (Table
All delimitation analyses recovered a congruence between morphological identifications and molecular information for all species (Fig.
Phylogenetic tree of species analyzed. Codes are referred to in Table
Congruently, the ABGD analysis recovered the undescribed taxon as a separate species from other Procambarus species. The bPTP and GMYC species delimitation analyses separate sequences of such populations as a distinct taxon; GMYC confirms the latter observation as well as the specific status of the remaining Procambarus species (Fig.
Regarding the conservation assessment, in total, five populations for the species were recorded: populations from Álamos and Camelinas fall into one single 5–10 km2 quadrant, and Saldiveña, Río Verdito, and San Juanito each falls into their own 5 km2 quadrant. This resulted in a maximum area of occupancy of 25–35 km2. However, this would be extremely inaccurate, as the available area of habitat (small streams, probably fragmented by large-magnitude creeks) is much more reduced inside each quadrant. Consequently, we consider that a gross estimation of area of occupancy for the species would fall in less than 5 km2. Considering the factors aforementioned, we found a conservation status for Procambarus xihui of Critically Endangered (CR) based on the following criteria: B.2.a (habitat severely fragmented), B.2.b.ii (continuing decline in area of occupancy), and B.2.b.iv (continuing decline in number of locations).
Cambaridae Hobbs, 1942
Holotype:
male from I (
Body pigmented, eyes well developed. Rostrum lanceolate, concave, without lateral spines; antennal scale width 0.50–0.54 × in its length; areola of moderate width (0.22–0.23 × wide in length) with 2–4 large punctations in narrowest part; cervical spine absent, single, shallow branchiostegal spine; chela shorter than cephalothorax length, long and thin, length 0.87–0.89 × the length of cephalothorax and 0.28–0.31 × wide than long, narrow-ovate. Dactyl forming a concave profile in mesial margin, palm of chela with scattered tubercles, mesial surface with row of seven or eight tubercles, palm length 0.55–0.66 × in dactyl length; no lateral spines on carapace; postrostral ridges very strong and wide, forming a strong tubercle, provided with longitudinal groove along its laterodorsal margin, its apical extreme slightly overreaching carapace surface, not forming evident apical spine. Male with hooks on ischiopodites of the third and fourth pairs of pereiopods, those on third ischiopodite extending beyond basioischial articulation.
First pair of pleopods slightly asymmetrical, reaching coxopodite of third pereiopod, with shoulder on cephalic margin beginning at distal fifth; a row of setae from base to second third of pleopod, a second row of setae along mesial surface starting at mid-length and third row of setae along mesial surface starting on last quart and extending laterally to base of terminal processes, where it forms a tuft of plumose setae; mesial process spiniform, directed caudally and slightly mesially, cephalic process spiniform, acute, hood-like, directed caudomedial, upon central projection and hidden beneath apical tuft; central projection corneous, lamellate, hood-like, tip decidedly projecting mesially, forming a concave blade-like structure, distally folded in mesial direction and reaching beyond the remaining terminal elements; caudal process corneous, crest-like, running on caudomesial surface of pleopod tip, along longitudinal pleopod axis, mesiodistally directed, forming a lateral side of the concavity formed distally by the central projection, reaching bellow point of mesial process position in lateral view.
Preanular plate with strong tubercles in caudal margin, and with setae along its margin, both well projecting over annulus cephalic area. Annulus ventralis rather fusiform, with depression along median surface and sinus in shallow Z-shape. Endopodite and exopodite of uropods with strong distolateral spines and median ridge ending in small spine, not reaching endopodite margin.
(Figs
Procambarus xihui. All illustrations from holotype except for F which is from allotype A dorsal view of cephalothorax B lateral view of cephalothorax C lateral view of cheliped D basal podomeres of second to fifth pereiopods E Epistome F caudal view of annulus ventralis. Scale bars: 5 mm (A–C); 2 mm (D–F).
Postrostral ridges conspicuous and wide, forming a strong tubercle, provided with longitudinal groove along its laterodorsal side, its apical edge slightly overreaching carapace surface, not forming evident apical spine. Suborbital angle obtuse, one branchiostegal spine present. Surface of the carapace deeply punctuate.
Epistome broadly triangular, subsymmetrical, with cephalomedian projection well defined. Antennule with ventral spine on basal segment well developed. Antennal scale width 0.5 × its length, maximum width at ca. 0. 5 × length, with a ridge along lateral margin ending in a strong spine.
Chela long and thin, 0.89 × the length of carapace and 0.31 × wide as long, narrow-ovate, dactyl forming a concave profile in mesial margin. Chela scattered with numerous setose tubercles and crowded with numerous denticles. Mesial margin of palm with row of seven tubercles, opposable sides of both fingers with strong tubercles, seven stronger on proximal half of dactyl. Fingers gaping along their length. Lateral margin of dactyl with weak ridge of acute tubercles proximally and punctations distally. Tip of fingers forming strong pencils. Opposable margin of fixed finger with four tubercles on basal one-quarter and five punctations along second and third distal quarters.
Width of carpus of first pereiopod ca. 0.63 × in its length. Merus length 0.45 × in cephalothorax length, with scattered punctations in lateral surface, two rows of spike-like tubercles on mesial surface, stronger at distal half, apical spine present. Hooks on ischiopodites of third and fourth pereiopods, former well exceeding basioischial articulation, latter reaching it. Bases of coxopodites of fourth and fifth pereiopods with caudomesial boss projection, the former extending on wide prominence on caudoventrally surface, caudomedial oriented, setose around margin, the latter blade-like, mesially oriented, bare.
Abdomen slightly narrower than carapace, width 0.88 × in cephalothorax width. Protopodite of uropods with distolateral spines, endopodite and exopodite with strong distolateral spines and median ridge ending in small spine, not reaching endopodite margin. Dorsal side of telson with one median spine on each caudolateral corner.
(Fig.
Shorter and smaller chela, 0.66 × length of carapace and width 0.31 × length, mesial profile of dactyl straight. Four strong tubercles on proximal half of opposable side of dactyl. Two conspicuous tubercles on opposable side of fixed finger, one on distal third. Width of carpus of first pereiopod ca. 0.63 × its length. Shorter merus, 0.39 × cephalothorax length. Left dactyl abnormally small, shorter than fixed finger. No hooks on ischiopodites of pereiopods. Caudomesial boss only evident on fifth coxopodite, mesially projected.
Annulus ventralis as described in diagnosis (Fig.
(Table
Left chela 0.87 × the length of cephalothorax and width 0.28 × in its length, mesial surface of chela with a row of ten tubercles, palm 0.55 × in dactyl length. Right chela abnormally smaller. Opposable side of dactyl with five stronger tubercles on proximal side, lateral margin of dactyl with ridge of punctations. Opposable margin of fixed finger with five tubercles on basal quarter, two of them stronger, and punctate along distal half.
Carpus of first pereiopod ca. 1.35 × longer than wide. Shorter merus (0.41 × cephalothorax length). Shallow hooks on ischiopodites of third and fourth pereiopods, the former longer, none exceeding basioischial articulation.
Terminal elements of first pleopods not stylized, certain incipient development in mesial process and central projection, the latter together with caudal and cephalic processes mesially oriented.
The new species depicts certain variability in coloration among populations, but most individuals show a general brownish body background with lighter scattered spots along thorax and abdomen (Fig.
The specific epithet -xihui comes from the term used by natives from the region, (also known as the Pame people), to refer to themselves. The term also means ‘indigenous’ in the Pame language.
Except for Procambarus digueti and P. regiomontanus, which are clearly distinctive among the crayfish fauna of Mexico and used here as outgroups, the new species shares some traits with the remaining species included, most of them inhabiting the Pánuco River basin. Among those are the possession of hooks on the ischiopodites of third and fourth pereiopods and the first pair of pleopods reaching the coxa of third pereiopods. However, the new species can be readily distinguished from two other species included inhabiting the Pánuco basin, P. strenthi and P. roberti, based in the following characters (among others): in P. roberti, the first pleopods are asymmetrical and lack a cephalic shoulder, and it possess a subtriangular, laterally grooved caudal process abutting the caudal base of central projection, which is notably more reduced than the shown by P. xihui. In P. strenthi, the first pleopods of the male form I are also strongly asymmetrical, bearing a strong angular shoulder in the cephalic surface, a cephalic process broad and lamellate, a dentiform central projection and a smaller subtriangular caudal process.
More specifically, the new species is morphologically related to a group of species placed in the subgenus Ortmannicus by Hobbs (1972), although subgeneric groupings in Procambarus have not been recognized recently (
Among other differences, the new species can be separated from P. caballeroi as the latter possess a wider rostrum, a laminated, laterally flattened cephalic process, a crest-like caudal process whose apex ends in a spine-like structure that is caudodistally directed. Among the main differences with P. gonopodocristatus are that the latter possesses a caudal process in the form of a long blade arced along the caudolateral surface, when in P. xihui this process is longer and situated along the caudomesial surface of the pleopod. Procambarus caballeroi and P. gonopodocristatus inhabit other river basins, south of the TMVB. The two species that most resemble P. xihui are P. toltecae and P. hidalgoensis.
The new species can easily be differentiated from P. toltecae because the latter shows a different arrangement of the terminal elements of the first pleopod: most conspicuous are the caudal orientations of the cephalic and caudal processes as well as the central projection, the latter two forming a triangular projection which extends in caudally and forms a right angle to the longitudinal axis of the appendix. In P. toltecae, the central projection is the longest among the related species, while in P. xihui, the three most apical elements are directed mesially and the caudal process is blade-like and runs along the mesial side of the pleopod. We find that the new species is most similar to P. hidalgoensis, from which, however, clear differences can be noticed. In the latter, the mesial process is latero-distally oriented, while in P. xihui its orientation is caudal and slightly mesial; both show a central projection that is corneous and flattened, but its division in two elements in P. hidalgoensis is clear, one larger and distally projected and the other shorter, straight, and mesially projected, while in P. xihui the two elements are fused and no clear delimitation exists between them unless observed on electron microscopy; they form one concave blade-like structure, distally folded in a mesial direction. The caudal process is laminated in both species, but in P. hidalgoensis it is located mesiocaudally to central projection, while in P. xihui it is more laterally located, becoming the lateral side of the concavity formed by the central projection, also mesially directed. In vivo, a distinctive red coloration was recorded in the male form I of P. hidalgoensis with a contrasting blackish stripe running laterally of cephalothorax. In P. xihui, a dark stripe can be present, but it does not contrast as the body color is brownish (Fig.
The phylogenetic analysis partially agrees with deductions from morphological similarities. The new species is grouped in a clade with P. hidalgoensis: these two species inhabit small, first-order springs of the Pánuco basin, although P. xihui inhabits higher altitude parts of three different sub-basins (between 1,000 m and ca. 1,200 m): the Jalpan River (later a tributary of the Santa María sub-basin), the Tancuilín sub-basin, and Extoraz sub-basin (both tributaries of the Moctezuma River). On the other side, P. hidalgoensis inhabits similar habitats (at an altitude of 1,485 m) but from the Río Hule sub-basin, a southern component of the Moctezuma sub-basin. This clade is grouped with P. toltecae, which inhabits much lower altitudes (here collected from 273 m). Similarly, the Pánuco system is inhabited by the remaining species here included except for P. digueti and P. regiomontanus, but most of them are from distinct sub-basins or altitudes. Results shown here support that this region is a depositary of distinct clades of crayfish diversity in Mexico, which possibly reflects a complex biogeographic history for the genus in northeast Mexico, from which P. xihui is one additional component. Additional phylogenetic and biogeographic inferences are surely complex and beyond the scope of the present manuscript and will be treated in further work.
The new species inhabits an entirely included area in the SGBR. With certain variation among populations, habitats are headwater stream ecosystems, less than 1.5 meters wide, showing surface water intermittently along their course for most of the year, especially in small ponds that are 0.5–3 m wide with reduced water flow (Fig.
The characteristic physical and chemical parameters of their habitats are temperatures between 20 and 28 °C, dissolved oxygen content between 8 and 12 mg l-1, pH 7–8 , and water hardness 90–350 mg CaCO3 l-1. The terrestrial vegetation of the riverside where the crayfish populations were found is composed by riparian vegetation of Platanus mexicana, Taxodium mucronatum, and Salix species.
Headwater streams might be more vulnerable to disturbances in the surrounding catchment than other aquatic habitats, which relate to a higher risk of biodiversity loss (
Collections for populations from the new species were made in the year 2002 and attempted in 2019, covering nearly 20 years. The climatic conditions and intense use of water described above has probably been related to the dramatic change observed by us at the visited sites, in which three of the five streams were almost dry or completely modified. In June 2019, an attempt to collect with the same sampling effort used in 2002 was carried out at all sites. We failed to find any crayfish at Las Camelinas, Saldiveña, and San Juanito, and in the remainder, crayfish were at much lower abundances than previously recorded. Additionally, several mass mortalities of crayfish were recorded from some sites, produced by the use of pesticides in crops surrounding the small streams.
As seen by their location, most populations were found in separated streams which were not in contact with each other for most of the year or even for several years. Most of individuals were found in such small populations and face situations of high dryness, in which they are limited to a small number of pools, representing a high risk of local extinctions. If crayfish diversity is one of the most endangered among freshwater fauna in the world (
We thank Patricia Ornelas, Heriberto Pedraza R., Ma. Guadalupe Lara, Sandra Pedraza, Heriberto Pedraza L., Regina Pedraza, and Gael Pedraza for their guidance and help collecting several of the populations. Halan Ortíz-Herrera made took some of the measurements, Aslam Narváez made the illustrations. This work was financed by project 257263 granted by CONACyT and project IA205020 by DGAPA – PAPIIT, UNAM both granted to the first author. This work was also supported by a post-doctoral fellowship to VJB by DGAPA, UNAM. Micrographs were taken by Berenit Mendoza-Garfias at the Lab of Electron Microscopy of Biology Institute, UNAM. Some lab work was assisted by Christian Cárdenas Monroy. Some species were collected with the help of Leonardo García-Vázquez, Stephany Rodríguez, Sharif Rodríguez, and Carlos Garita; some populations from SGBR were sampled on previous occasions with the help of Alfredo Morales-Ortíz and Carlos Ramírez.
Results of the ABGD, bPTP and GMYC species delimitation analysis
Data type: Delimitation analyses
Table S1
Data type: Genetic distances
Explanation note: Uncorrected P-distances for the COI fragment between species included in this study (in bold) and standard error.