Research Article |
Corresponding author: Peter K. L. Ng ( peterng@nus.edu.sg ) Academic editor: Célio Magalhães
© 2020 Peter K. L. Ng.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Ng PKL (2020) Revision of the freshwater crabs of the Johora tahanensis (Bott, 1966) species group (Crustacea, Brachyura, Potamidae), with a key to the genus. ZooKeys 994: 1-34. https://doi.org/10.3897/zookeys.994.56810
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The taxonomy of the potamid freshwater crabs of the Johora tahanensis (Bott, 1966) species group (Potamoidea) is revised. Seven species are recognised, all from Peninsular Malaysia and southern Thailand, three of which are described as new. The three new species were previously identified as J. tahanensis but can be distinguished by characters of the carapace, male first gonopod, and vulva. A revised key to the 18 recognised species of Johora Bott, 1966, is provided.
Freshwater crab, new species, Peninsular Malaysia, Potamoidea, taxonomy, Thailand
Six genera of potamid freshwater crabs are known from the Malay Peninsula (Peninsular Malaysia, Thailand south of the Isthmus of Kra, and Singapore): Baccazia Ng, 2018a, Demanietta Bott, 1966, Gempala Ng & Ahmad, 2016, Johora Bott, 1966, Stoliczia Bott, 1966, and Terrapotamon Ng, 1986a (see
Johora is one of the most diverse of these Malayan genera, with 15 known species: J. aipooae (Ng, 1986a), J. counsilmani (Ng, 1985), J. gapensis (Bott, 1966), J. grallator Ng, 1988, J. gua Yeo, 2001, J. hoiseni Ng & Takeda, 1992, J. intermedia (Ng, 1986b), J. johorensis (Roux, 1936), J. murphyi (Ng, 1986b), J. punicea (Ng, 1985), J. singaporensis (Ng, 1986b), J. tahanensis (Bott, 1966), J. thaiana Leelawathanagoon, Lheknim & Ng, 2005, J. thoi Ng, 1990, and J. tiomanensis (Ng & Tan, 1984) (
The phylogenetic study by
Measurements provided are of the maximum carapace width and length (in millimetres), respectively. The abbreviations G1 and G2 are used for the male first and second gonopods, respectively. The terminology used follows
A full description is given for Johora tahanensis; for all other taxa, only diagnoses are provided.
Potamon (Potamon) johorense Roux, 1936, by original designation.
Johora johorensis (Roux, 1936): 1 male, 1 female, 1 juvenile (
Members of the J. tahanensis species group are distinct from the nominate species group (J. johorensis, J. gapensis, J. tiomanensis, J. counsilmani, J. intermedia, J. murphyi and J. singaporensis) (sensu
In recent years, the structure of the vulva has proven useful in potamid taxonomy for some groups, but for Johora species, its morphology appears to be relatively conservative. The one exception is J. thoi, where the lateral sternal vulvar cover projects obliquely anteriorly as a large triangular plate (Fig.
Overall dorsal view A Johora intermedia Ng, 1986, holotype male (19.8 × 15.1 mm) (
The molecular study by
The last key to the genus was constructed in 1988 and in view of the additional species described since, there is a necessity to update this, and a revised key is presented here.
Dorsal view of carapace A Johora intermedia Ng, 1986, holotype male (19.8 × 15.1 mm) (
Potamiscus (Johora) johorensis tahanensis Bott, 1966: 495 (part), pl. 21 fig. 15.
Stoliczia (Johora) johorensis tahanensis
–
Johora tahanensis
–
Stoliczia johorensis tahanensis
–
13 males (largest 27.2 × 22.9 mm), 13 females (largest 24.4 × 21.0 mm), 4 juveniles (
Adult carapace width to length ratio 1.16–1.19 (Figs
Frontal view of cephalothorax A Johora intermedia Ng, 1986, holotype male (19.8 × 15.1 mm) (
Carapace subrectangular broader than long, width to length ratio 1.16–1.19; dorsal surface gently convex in frontal view (Figs
Third maxillipeds covering most of buccal cavity when closed; surfaces of merus, ischium and exopod with numerous long stiff setae; ischium subrectangular, with shallow median oblique groove; merus subquadrate, slightly wider than long, anteroexternal angle not expanded; exopod slender, reaching half length of merus, with long flagellum (Figs
Left third maxilliped A Johora intermedia Ng, 1986, holotype male (19.8 × 15.1 mm) (
Chelipeds asymmetrical (Fig.
Ambulatory legs not elongate, length to width ratio of merus of fourth ambulatory leg 2.7–2.8; second pair longest, fourth pair shortest (Fig.
Thoracic sternum (notably sternites 3, 4) with shallow pits to smooth (Fig.
Anterior thoracic sternum and pleon A Johora intermedia Ng, 1986, holotype male (19.8 × 15.1 mm) (
Pleon triangular, all somites, telson free; telson triangular, lateral margins almost straight to gently sinuous; somite 6 subtrapezoidal, distinctly wider than long, lateral margins gently sinuous; somites 3–5 trapezoidal, gradually decreasing in width; somites 1, 2 subrectangular, narrow, very wide, reaching to bases of coxae of fourth ambulatory legs, thoracic sternite 8 not visible when pleon closed (Fig.
G1 subterminal segment gradually tapering from broad proximal part to slender distal part, without distinct shelf-like structure along gently concave outer margin; terminal segment gently curved outwards (from median part of sternum), ca. half length of subterminal segment, surfaces with numerous short setae (Fig.
Female. Similar to male in most non-sexual features; chelipeds symmetrical or only slightly asymmetrical (Fig.
The material reported as “J. tahanensis” by
Johora tahanensis is known from the tributaries around Sungei Tahan in southern Pahang, at the southern part of Taman Negara National Park (Fig.
The species is not under any immediate threat as it is found in Malaysia’s oldest and largest national park.
Johora thoi
Ng, 1990: 305, figs 1, 2;
Holotype
: male (41.2 × 33.3 mm) (
Adult carapace width to length ratio 1.19–1.24 (Figs
This is one of the largest species of Johora and is rivalled in size only by J. tiomanensis and J. counsilmani from Pulau Tioman. The G1 structure of J. thoi is distinctive and consistent, the long and straight terminal segment being evident even in small subadult specimens (Fig.
A–D Johora tahanensis (Bott, 1966), male (30.7 × 25.5 mm) (
A–D Johora thoi Ng, 1990, holotype male (41.2 × 33.3 mm) (
Johora thoi is endemic to Pulau Redang, the largest island in the Redang Archipelago, a group of nine islands about 25 km off the northeastern coast of Peninsular Malaysia. The species has been recorded from most parts of the island, including its highest point (359 m), occurring in all clean waters there (Fig.
Although the entire Redang Archipelago is a marine park, the forests are not fully protected, and development of the land for tourism and excessive freshwater use are concerns. As the species is only known from one island only 7 km long and 6 km wide, it is treated as endangered by
Potamiscus (Johora) johorensis tahanensis Bott, 1966: 495 (part), fig. 32 (not Stoliczia (Johora) johorensis tahanensis Bott, 1966 s. str.).
Stoliczia (Johora) johorensis tahanensis
–
Johora tahanensis
–
Johora hoiseni
Ng & Takeda, 1992: 108, figs 2, 3;
Holotype
: male (25.1 × 21.1 mm) (
Adult carapace width to length ratio 1.16–1.19 (Figs
Johora hoiseni is known from the drainages near Gua Musang in Kelantan, in the northwestern part of Taman Negara National Park (Fig.
The species is not under any immediate threat as it is found in Malaysia’s oldest and largest national park.
Johora thaiana
Leelawathanagoon, Lheknim & Ng, 2005: 60, figs 1, 2;
Paratypes
: 1 male (22.2 × 18.6 mm) (
Adult carapace width to length ratio 1.10–1.21 (Figs
A–D Johora thaiana Leelawathanagoon, Lheknim & Ng, 2005, paratype male (22.2 × 18.6 mm) (
The G1 structure of J. thaiana most closely resembles that of J. hoiseni in that the terminal segment is straight; but their subterminal segments differ. In J. thaiana, the subterminal segment gradually tapers from the broad proximal part to a slender distal section, without a shelf-like structure along the outer margin (Fig.
A–D Johora erici sp. nov., holotype male (38.3 × 32.0 mm) (
Johora thaiana is known from the forest tributaries in southern Thailand, near the Malaysian border at Kelantan (Fig.
The species has so far only been found in protected forests in southern Thailand and is not under any immediate threat;
Johora tahanensis – Takeda & Ng, 1992: 107 (part); Ng 1995: 249 (part) (not Stoliczia (Johora) johorensis tahanensis Bott, 1966).
Holotype
: male (34.6 × 30.0 mm) (
Adult carapace width to length ratio 1.15–1.17 (Figs
Overall dorsal view A Johora intermedia Ng, 1986, female (17.9 × 14.7 mm) (
The species is named after an old friend and mentor, the late Dr Lim Boo Liat (1926–2020), one of Malaysia’s most eminent biologists and naturalists. In a remarkable career spanning 70 years, he has contributed immensely to biodiversity and conservation science in Malaysia; more importantly, he has inspired, mentored, and trained many generations of biologists, many who now lead science in Malaysia and Singapore.
The large adult size of J. booliati sp. nov. and J. erici sp. nov. (carapace width in excess of 25 mm) with the pronounced epibranchial teeth allies them with J. tahanensis s. str., J. hoiseni, J. thaiana, J. thoi, J. tiomanensis, and J. counsilmani (the last two being endemic to Pulau Tioman, Peninsular Malaysia).
Johora booliati and J. erici resemble J. tahanensis, J. hoiseni, and J. thaiana in the carapace shape and general features. Their G1 structures, however, are different. In J. booliati and J. erici, the G1 subterminal segment is proportionately more elongate and the terminal segment is relatively shorter (Figs
Johora booliati and J. erici can be distinguished from J. tiomanensis and J. counsilmani by their carapace being relatively more quadrate in shape (width to length ratio less than 1.2) and the adult carapace is distinctly wider than long (width to length ratio 1.3–1.4) in J. tiomanensis and J. counsilmani. In addition, the epigastric cristae of J. booliati and J. erici are distinctly anterior to the postorbital cristae and separated by a gap with the entire postorbital cristae sharp (Figs
The G1 structure of J. booliati closely resembles that of J. erici, with the terminal segments similar in shape and proportions. The G1 subterminal segments of the two species, however, differ in form, with that of J. booliati forming a shelf-like structure along the outer margin (Fig.
The G1 structure of J. booliati and J. erici is superficially similar to that of J. murphyi from southern Peninsular Malaysia, notably in the shape of the G1 terminal and subterminal segments (
Johora booliati sp. nov. is known from highland streams in the central highlands of Pahang (Genting Highlands, Bukit Tinggi and Fraser’s Hill) (Fig.
The conservation status for J. booliati is not known as its actual distribution is not known. For the moment, it is known only from a 30 km stretch of hills along the Central Highlands of Peninsular Malaysia, in the area of Fraser’s Hill, Genting Highlands and Bukit Tinggi. This area is not protected, and in this context, the species should be categorised as vulnerable for the time being (see
Johora tahanensis
– Ng, 1995: 249 (part), fig. 1;
Holotype
: male (38.3 × 32.0 mm) (
Adult carapace width to length ratio 1.20–1.22 (Figs
Female pleon A Johora intermedia Ng, 1986, female (17.9 × 14.7 mm) (
The species is named after the first post-independence director of the National Museum of Singapore (the renamed Raffles Museum) (1967–1973), the late Eric Alfred. A very active freshwater ichthyologist, he collected many of the freshwater crabs in the museum. Eric was a good friend and provided wise counsel and help even years after he stepped down and took over the directorship of the Singapore Maritime Museum.
Ng (1995: 250) commented that the G1 terminal segment of the males from Perak “seems to be somewhat longer and more slender” compared to typical J. tahanensis but incorrectly attributed it to variation. The differences are actually consistent, and it is here recognised as a separate species, J. erici sp. nov. See remarks for J. booliati sp. nov. for differences with allied taxa.
Johora erici sp. nov. is known thus far only from highland streams in northern Perak and Kelantan (Fig.
The conservation status for J. erici is unclear as we have too few specimens, although it appears to have a relatively wide range in the mountains. It should be best categorised as data deficient for the time being (see
Holotype
: male (22.7 × 19.2 mm) (
Adult carapace width to length ratio 1.20–1.22 (Figs
Sternopleonal cavity and vulvae A Johora intermedia Ng, 1986, female (17.9 × 14.7 mm) (
The species is named after the last director of the Raffles Museum (1946–1967), the late Michael Tweedie, an intrepid collector of interesting animals from Malaysia. The author had the pleasure of knowing him, finally meeting him when the refreshed museum opened as the
The holotype male of Johora michaeli sp. nov. is not fully adult and it is clear that it can grow larger, with the adult females reaching 30 mm in carapace width. The G1, however, remains diagnostic, with the terminal segment elongate and almost straight (Fig.
The G1 of J. michaeli also resembles that of J. singaporensis but the terminal segment in this species is longer (
One large female (
Johora michaeli sp. nov. is known so far only from highland streams in central Terengganu (Fig.
The conservation status for J. michaeli should be regarded as vulnerable as it is known from a relatively well protected area that is not subject to development (see
1 | Carapace with anterolateral margins strongly convex, branchial regions appear gently swollen, external orbital tooth very acutely triangular; length of flagellum of third maxilliped exopod subequal to width of merus; G1 very stout, terminal segment cone-shape, tapering to relatively sharp tip (Pahang, Malaysia) | Johora aipooae (Ng, 1986a) |
– | Carapace with anterolateral margins gently convex, branchial regions not swollen, external orbital tooth broadly triangular; flagellum on third maxilliped exopod longer than width of merus; G1 slender, variable shapes | 2 |
2 | Frontal regions narrow, appear compressed, frontal margin slightly below level of external orbital tooth in dorsal view; postorbital cristae sharp, extending to epibranchial tooth as one structure; ambulatory legs especially, very long, length of merus more than 5 times maximum width (highlands, above 750 m a.s.l., Pulau Tioman, Malaysia) | Johora grallator Ng, 1988 |
– | Frontal region not distinctly narrow, frontal margin level with external orbital tooth; postorbital cristae sharp or low, never extending to epibranchial tooth, if joining always through series of interrupted striae; ambulatory legs not prominently elongate, length of merus less than 4.5 times maximum width | 3 |
3 | Epigastric cristae just slightly anterior of and almost confluent with or indistinctly separated from postorbital cristae; postorbital cristae distinct not high, usually more prominent along median part of carapace, becoming uneven or breaking up into striae and granules laterally, not clearly reaching cervical groove | 4 |
– | Epigastric cristae prominently anterior of and clearly separated from postorbital cristae; postorbital cristae high, sharp along entire length to cervical groove | 12 |
4 | Dorsal carapace surface usually smooth, striae when present very low, at most with scattered very short setae; G1 terminal segment very slender, hook-shaped, subterminal segment neck-like with stout base; carapace and appendages purplish-red or uniformly orange in life (Pulau Tioman, Malaysia) | 5 |
– | Dorsal carapace surface with scattered granules and striae especially along lateral margins, usually with numerous short stiff setae; Gl terminal segment relatively stouter, various shapes, subterminal segment stout; carapace and appendages brown with patches of pale orange in life (Pulau Tioman and rest of Malay Peninsula) | 6 |
5 | Ambulatory legs relatively shorter, stouter (dactylus of second leg 5.9–7.5 times longer than broad; second ambulatory leg merus 3.5–4.0 times longer than broad); purplish-red in life (lowland species, 100–300 m a.s.l., Pulau Tioman, Malaysia) | Johora punicea (Ng, 1985) |
– | Ambulatory legs relatively slenderer (dactylus of second leg 11.4–15.0 times longer than broad; second ambulatory leg merus 4.3–4.4 times longer than broad); purplish-red in life (montane species, ca. 900 m a.s.l., highlands, Pulau Tioman, Malaysia) | Johora gua Yeo, 2001 |
6 | Large species (adult carapace width 30–45 mm); adults with anterolateral margin strongly convex; frontal and anterolateral regions covered with numerous granules and striae (Pulau Tioman, Malaysia) | 7 |
– | Small species (adult carapace width 22–23 mm); adults with anterolateral margin gently convex; frontal and anterolateral regions covered with scattered granules and striae (mainland Peninsular Malaysia) | 8 |
7 | Adult G1 terminal segment prominently hook-shaped, evenly tapering to tip, longer than half length of subterminal segment, surfaces almost smooth or with low flap, indistinct cleft between terminal and subterminal segments (western Pulau Tioman, Malaysia) | Johora tiomanensis (Ng & Tan, 1984) |
– | Adult G1 terminal segment slightly sinuous along distal half, shorter than half length of subterminal segment, with distinct flap on distal part of upper margin, distinct broad cleft between terminal and subterminal segments (eastern Pulau Tioman, Malaysia) | Johora counsilmani (Ng, 1985) |
8 | G1 straight, slender, terminal segment rod-shaped, as long as subterminal segment, medium size species (Singapore) | Johora singaporensis Ng, 1986b |
– | G1 bent in varying degrees between terminal and subterminal segment, terminal segment shorter than subterminal segment, tapered, downcurved and hook-shaped, small to large species (Malaysia) | 9 |
9 | G1 terminal segment gently but distinctly upcurved (Gunong Pulai, Johor, Malaysia) | Johora johorensis (Roux, 1936) |
– | G1 terminal segment straight or hook-shaped | 10 |
10 | G1 terminal segment prominently curved, sickle-shaped, strongly bent, longer than half length of subterminal segment (Central Highlands, Malaysia) | Johora gapensis (Bott, 1966) |
– | G1 terminal segment gently curved, hook-shaped, obliquely bent, half or less than half length of subterminal segment | 11 |
11 | G1 terminal segment curved, about half length of subterminal segment, with broad cleft between terminal and subterminal segments (Gunong Panti and adjacent highlands, Malaysia) | Johora murphyi Ng, 1986b |
– | G1 terminal segment slightly curved, tapered, less than half length of subterminal segment, wide ranging subspecies (Selangor, Negeri Sembilan, eastern Pahang, northern Johor, Malaysia) | Johora intermedia Ng, 1986b |
12 | G1 terminal segment straight to almost straight, long, rod-like, at least two-thirds length of subterminal segment | 13 |
– | G1 terminal segment straight to curved and hook-shaped, never as long as subterminal segment | 14 |
13 | Ambulatory legs relatively longer, length to width ratio of merus of fourth ambulatory leg 3.0–3.2; G1 terminal segment very long, slender, subequal to length of subterminal segment, even in young males (Pulau Redang, Malaysia) | Johora thoi Ng, 1990 |
– | Ambulatory legs relatively shorter, length to width ratio of merus of fourth ambulatory leg 2.7–2.8; G1 terminal segment almost straight, about two-thirds length of subterminal segment, even in young males (Terengganu, Malaysia) | Johora michaeli sp. nov. |
14 | G1 terminal segment gently curved outwards | 15 |
– | G1 terminal segment straight or nearly so | 17 |
15 | Adult posterior carapace margin with shallow median indentation; G1 subterminal segment with broad proximal part, tapering relatively suddenly to slender distal part, with low shelf-like structure on outer margin (Bukit Tinggi, Genting Highlands and Fraser’s Hill, Malaysia) | Johora booliati sp. nov. |
– | Adult posterior carapace margin gently convex, entire; G1 subterminal segment gradually tapering from broad proximal part to slender distal part, without distinct shelf-like structure along gently concave outer margin (Pahang, Kelantan and Perak, Malaysia) | 16 |
16 | Frontal margin almost straight; G1 subterminal segment relatively stouter, terminal segment proportionately shorter, stouter (Pahang) | Johora tahanensis (Bott, 1966) |
– | Frontal margin sinuous; G1 subterminal segment relatively slenderer, terminal segment proportionately longer, slenderer (Kelantan and Perak, Malaysia) | Johora erici sp. nov. |
17 | G1 terminal segment with margins subparallel or gently converging for most of length, tip straight to gently curving upwards (Kelantan, Malaysia) | Johora hoiseni Ng & Takeda, 1992 |
– | G1 terminal segment distinctly tapering, forming conical structure, distal part slightly curved (southern Thailand) | J. thaiana Leelawathanagoon, Lheknim & Ng, 2005 |
Thanks are due to Evan Quah, Ruth Mary Low, Martyn Low, and Kelvin Lim for helping collect many of the more recent specimens of the new taxa. The paper has benefitted from the many helpful comments by Célio Magalhães, Jose Christopher Mendoza, and Tohru Naruse.