Research Article |
Corresponding author: Dandan Zhang ( zhdd61@163.com ) Academic editor: Bernard Landry
© 2020 Dandan Zhang, Kai Chen, Lanbin Xiang.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Zhang D, Chen K, Xiang L (2020) Revision of the genus Epiparbattia Caradja, 1925 (Lepidoptera, Crambidae, Pyraustinae), based on morphology and molecular data. ZooKeys 960: 143-155. https://doi.org/10.3897/zookeys.960.54986
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The genus Epiparbattia Caradja, 1925 is revised based on general appearance, including genitalia. A new species, Epiparbattia multispinalis Zhang & Chen, sp. nov. is described. The external characters and genitalia morphology of all species are figured. The phylogeny of Epiparbattia species is investigated using molecular data. Monophyly of the genus is well supported by phylogenetic analysis based on sequence data of COI, 16S rRNA, EF-1α and 28S rRNA gene regions.
China, Epiparbattia, molecular phylogeny, new species, Sclerocona, taxonomy
The genus Epiparbattia Caradja, 1925 comprises only two species, E. gloriosalis Caradja, 1925 and E. oligotricha Zhang & Li, 2005, distributed in southern China, India and Bhutan (
The material studied, including the types of the newly described species, are all deposited at the Museum of Biology, Sun Yat-sen University, China (SYSBM) except those specified as being in the Insect Collection of the College of Life Sciences, Nankai University, China (
In total seven species of four genera were included for molecular phylogenetic analyses (Table
Genus | Species | Voucher no. | Locality | Genbank accession number | References | |||
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COI | 16S | EF-1α | 28S | |||||
Euclasta | stoetzneri | SYSU-LEP0334 | Shaanxi | MT738696 | MT734412 | MT724335 | MT734404 | Present study |
Crocidophora | lutusalis | SYSU-LEP0088 | Yunnan | MT738697 | MT734413 | MT724336 | MT734405 | |
pallidulalis | SYSU-LEP0090 | Yunnan | MT738698 | MT734414 | MT724337 | MT734406 | ||
Epiparbattia | oligotricha | SYSU-LEP0243 | Sichuan | MT738699 | MT734415 | N/A | MT734407 | |
oligotricha | SYSU-LEP0359 | Yunnan | MT738700 | MT734416 | MT724338 | MT734408 | ||
multispinalis | SYSU-LEP0351 | Hubei | MT738701 | MT734417 | N/A | MT734409 | ||
multispinalis | SYSU-LEP0378 | Hubei | MT738702 | MT734418 | MT724339 | MT734410 | ||
gloriosalis | SYSU-LEP0244 | Guangdong | MT738703 | MT734419 | MT724340 | MT734411 | ||
Sclerocona | acutella | SYSU-LEP0152 | Macao | MG739577 | MG739589 | MG739601 | MG739612 |
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The sequences were aligned using Clustal W (
Epiparbattia Caradja, 1925: 358.
Epiparbattia gloriosalis Caradja, 1925, by monotypy.
Diagnosis. This genus is related to Sclerocona Meyrick, 1890 in the forewings bearing a fovea basally beyond the cell and another fovea between R3+4 and R5, as well as the minute basal and apical outer spurs of the hindlegs in the male, the developed and sclerotized lamella postvaginalis, the coiled and partly sclerotized posterior part of the ductus bursae and the second signum with two almost parallel ridges in the female genitalia, but can be distinguished by the larger body size, the relatively short labial palpi and the prominent markings on the wings. In the genitalia, Epiparbattia differs from Sclerocona by the relatively broad uncus, the uninflated sacculus, the stout, ventrodistally sclerotized phallus and the broad, nearly elliptical signum.
Redescription. Frons rounded, sometimes weakly flat. Labial palpi obliquely porrect, third segment porrect; exceeding frons by less than the diameter of eyes. Maxillary palpi slightly broadened terminally. Hindlegs of male with basal and apical outer spurs minute. Forewings elongated triangular, costa slightly curved near apex, apex obtuse, termen slightly curved and oblique, tornus rounded; reniform stigma developed and connected with postmedial band, postmedial band comprising of a series of patches and with a broad patch on posterior margin, subterminal band comprising of a series of broad, elliptical patches; length of cell less than half of forewing, male with posterior margin of cell, CuA2 and 1A basally curved, and forming a fovea, with a scale-tuft on the underside surface at position of fovea, R3 and R4 stalked about 1/2–2/3 length of R4, R5 basally curved, a fovea present between R3+4 and R5 but without scale-tuft on the underside surface at position of fovea. Hindwings fan-shaped; length of cell less than half length of hindwing; Sc+R1 and Rs anastomosed for 1/3–1/2 length of Rs; subterminal band narrow. Male genitalia. Uncus nearly triangular, distally narrowly rounded, with distal half densely setose laterally and dorsally, basal half sparsely setose laterally. Tegumen with dorsal 1/3–1/2 narrow and basal 2/3–1/2 broad. Transtilla connected, arms nearly triangular or trapezoidal, sparsely bearing slender setae, with ventral process extending to juxta. Valvae tongue-shaped, costa nearly straight, ventral margin convex, apex rounded; sella weakly sclerotized and setose, dorsally bearing a large cluster of curved and thick setae or several slender setae forming an editum, ventrally with strongly sclerotized processes; sacculus not inflated; saccus nearly triangular. Juxta with dorsal part divided into two arms. Phallus stoutly cylindrical, distally with ventral part sclerotized. Female genitalia. Ovipositor lobes flat, densely setose. Apophyses stout, anterior apophyses about same length of posterior apophyses, 8th tergite with base of anterior apophysis strongly extended forward and connected with lamella postvaginalis. Lamella postvaginalis developed, sclerotized. Antrum reduced. Ductus bursae usually longitudinally wrinkled, about 2–3 times length of corpus bursae; with most of posterior part coiled and partially sclerotized, usually inflated; colliculum elongated hourglass-shaped; ductus seminalis arising closely from anterior end of colliculum. Corpus bursae globular or oval, wrinkled; appendix bursae originating from posterior part; signum broadly rhomboid, nearly elliptical; second signum located between base of appendix bursae and entrance of ductus bursae, plate-shaped and curved, usually with two almost parallel ridges.
Distribution. China, India, Bhutan.
1 | Forewings ground colour pale yellow and the covering dark brown scales forming markings in male (Fig. |
E. multispinalis Zhang & Chen, sp. nov. |
– | Wings creamy white bearing dark brown markings; ventral processes of sella with the dorsalmost one curved and with sparse spines ventrally | 2 |
2 | Forewings with postmedial band interrupted (Fig. |
E. gloriosalis Caradja, 1925 |
– | Postmedial band of forewing not interrupted (Fig. |
E. oligotricha Zhang & Li, 2005 |
Epiparbattia gloriosalis Caradja, 1925: 359.
Epiparbattia gloriosalis whalleyi Munroe & Mutuura, 1971: 506.
Paratype
of Epiparbattia gloriosalis Caradja, 1925: 1♀, [China: Guangdong]: Lienping [Lianping], 26. April (
China: Fujian: 1♂, Sangang, 15.VIII.1979 (
Wingspan 32.0–41.0 mm. This species is similar to Epiparbattia oligotricha in appearance, but can be distinguished from it by the tegulae with only one black spot at base, the interrupted postmedial band and the narrower patch on the posterior margin; in male genitalia by the dorsal side of the sella densely covered with thick setae forming editum, and the setae subapically curved and divided into several filaments, the ventral processes of the sella with the dorsalmost curved inward, apically bifurcated, the ventralmost curved ventrad, and by the longer arms of the juxta; in female genitalia by the absence of a U-shaped concave unsclerotized window of the lamella postvaginalis anteriorly and the uninflated posterior part of the ductus bursae.
China (Fujian, Hubei, Guangdong, Guangxi, Sichuan, Yunnan, Xizang), India, Bhutan.
Larvae bore in the stems of bamboo shoots of Sinocalamus affinis (Rendle) McClure (
Epiparbattia oligotricha Zhang & Li, 2005: 40.
Holotype
♂, China: Guizhou: Mt. Fanjingshan, 27°33'N, 108°24'E, alt. 1700 m, 1.VI.2002, leg. Wang Xinpu (
China: Sichuan: 5♀♀, Labahe, Tianquan, 30.09N, 102.52E, alt. 1860 m, 8.VII.2012, leg. Li Jinwei, genitalia slide no. SYSU1035, molecular voucher no. SYSU-LEP0243, SYSU-LEP0335; Yunnan: 1♂, Qinlangdang Reserve Station, Gaoligongshan Reserve, Nujiang, 27.69°N, 98.27°E, alt. 380 m, 30.V.2017, leg. Teng Kaijian et al., genitalia slide no. ZDD12109, molecular voucher no. SYSU-LEP0359 (
Wingspan 32.0–47.0 mm. This species is superficially similar to Epiparbattia gloriosalis, but can be distinguished from it by the tegulae bearing a second large black spot in the center, the large orbicular stigma, the uninterrupted postmedial band and the wider patch of the postmedial band at the posterior margin; in male genitalia by the dorsal side of the sella sparsely covered with slender simple setae forming editum, the ventral processes of the sella with the dorsalmost curved dorsad and with ventral margin sparsely bearing spines, the ventralmost slightly curved inward, as well as the shorter arms of the juxta; in female genitalia by the presence of a deep, U-shaped concave unsclerotized window of the lamella postvaginalis anteriorly and the inflated posterior part of the ductus bursae.
Holotype
♂, China: Hubei: Shuangping, Zhuxi, 31.57N, 109.87E, alt. 1201 m, 5.VII.2017, leg. Qi Wanding, genitalia slide no. ZDD12101, molecular voucher no. SYSU-LEP0378 (
The new species differs from the other two species by the pale yellow ground colour of forewings with dark brown markings in the male and the pale-yellow wings in the female. In the male genitalia, Epiparbattia multispinalis is similar to E. oligotricha, but differs from the latter in the concave lateral margin of the uncus, the more convex ventral margin of the valva, the ventral processes of the sella with the dorsalmost straight and long, narrowly triangular, with rows of spines ventrally, transversely extending inward, and by the large drop-shaped cornutus. In the female genitalia, this species is different from E. gloriosalis and E. oligotricha by the prominently inflated posterior part of the ductus bursae, approximately 2–3 times the width of the remainder of the ductus bursae.
Wingspan 29.0–33.0 mm. Male (Fig.
The specific name is derived from the Latin multi- (= many) and spinalis (= spine) corresponding to the ventral processes of the sella bearing many spines.
China (Hubei).
The concatenated dataset of four genes consisted of 2510 nucleotide positions (658 for COI, 468 for 16S rRNA, 619 for 28S rRNA, and 765 for EF-1α). Both BI and ML analyses of the concatenated dataset inferred congruent topologies with only subtle differences in posterior probability and bootstrap values (Fig.
Pairwise distances of the barcoding region (COI) are given in Table
Pairwise distances of the COI barcode region based on Kimura-2-parameter model (intraspecific distances are highlighted in bold).
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||
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1 | SYSU-LEP0334 Euclasta stoetzneri | ||||||||
2 | SYSU-LEP0088 Crocidophora lutusalis | 0.133 | |||||||
3 | SYSU-LEP0090 Crocidophora pallidulalis | 0.136 | 0.077 | ||||||
4 | SYSU-LEP0243 Epiparbattia oligotricha | 0.138 | 0.098 | 0.085 | |||||
5 | SYSU-LEP0359 Epiparbattia oligotricha | 0.133 | 0.101 | 0.089 | 0.019 | ||||
6 | SYSU-LEP0351 Epiparbattia multispinalis | 0.153 | 0.103 | 0.098 | 0.056 | 0.070 | |||
7 | SYSU-LEP0378 Epiparbattia multispinalis | 0.155 | 0.105 | 0.099 | 0.058 | 0.071 | 0.002 | ||
8 | SYSU-LEP0244 Epiparbattia gloriosalis | 0.135 | 0.098 | 0.089 | 0.072 | 0.079 | 0.067 | 0.069 | |
9 | SYSU-LEP0152 Sclerocona acutella | 0.118 | 0.113 | 0.092 | 0.092 | 0.103 | 0.087 | 0.089 | 0.096 |
The monophyly of Epiparbattia is robustly supported by the results of the molecular analysis. Three species can be recognized as members of Epiparbattia by a series of external and genital characters provided above in the diagnosis of the genus. As is apparent from the tree topology (Fig.
Additionally, several pyraustine genera, Anamalaia Munroe & Mutuura, 1969, Lepidoplaga Warren, 1895, Limbobotys Munroe & Mutuura, 1970 and Torulisquama Zhang & Li, 2010, are similar to Epiparbattia and Sclerocona by bearing fovea (at least one fovea) on the forewing, minute basal and apical outer spurs of hindleg in males, as well as a developed and sclerotized lamella postvaginalis in females, but can still be distinguished from each other by the number and position of the fovea and other genital characters. The relationships among all these genera need to be further studied.
Grateful thanks go to Prof. Houhun Li (