Research Article |
Corresponding author: Andrzej Falniowski ( andrzej.falniowski@uj.edu.pl ) Academic editor: Eike Neubert
© 2020 Sebastian Hofman, Aleksandra Rysiewska, Artur Osikowski, Andrzej Falniowski.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Hofman S, Rysiewska A, Osikowski A, Falniowski A (2020) A new species of Kerkia Radoman, 1978 (Caenogastropoda, Hydrobiidae) from Bosnia and Herzegovina. ZooKeys 973: 17-33. https://doi.org/10.3897/zookeys.973.52788
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A new species of Kerkia, K. briani Rysiewska & Osikowski, sp. nov. is described from the spring Polički Studenac Vrelo (Crkvina), adjacent to the Trebišnjica River (Bosnia and Herzegovina) collected with Bou-Rouch technique, pumped from an interstitial habitat 50 cm below the bottom of the spring. The shell, female reproductive organs, and the penis are described and illustrated. Mitochondrial cytochrome oxidase subunit I (COI) and nuclear histone H3 partial sequences confirm the distinctness of the new species, and molecularly based phylogenetic relationships of Kerkia are briefly presented.
Balkans, cytochrome oxidase, Gastropoda, histone, interstitial, molecular taxonomy, morphology, stygobiont
Mud snails Hydrobiidae are very small or minute snails, whose shells are often approximately 1 mm high. They inhabit surface and subterranean freshwater habitats, although some can also be found in brackish and even marine environments. The family comprises more than 400 extant genera (
In September of 2019, in the spring Polički Studenac Vrelo (Crkvina), adjacent to the Trebišnjica River, we found Emmericia expansilabris Bourguignat, 1880, Sadleriana sp., Anagastina vidrovani (Radoman, 1973), and Ancylus recurvus Martens, 1873. Pumping of the interstitial fauna from sediments below the spring resulted in the collection of a few most probably stygophilic Radomaniola, but also the typically stygobiont Montenegrospeum Pešić & Glöer, 2013 and Kerkia. The representatives of the latter genus did not belong to any species known so far, and in the present paper we describe this new species and discuss its relationships.
The snails were collected at the spring Polički Studenac Vrelo (Crkvina), adjacent to the Trebišnjica River (42°42'46.4"N, 18°21'54.5"E), near Trebinje, Bosnia and Herzegovina (Fig.
The shells were photographed with a Canon EOS 50D digital camera, under a Nikon SMZ18 microscope. The dissections were done under a Nikon SMZ18 microscope with dark field, equipped with Nikon DS-5 digital camera, whose captured images were used to draw anatomical structures with a graphic tablet. Measurements of the shell (Fig.
Snails for molecular analysis were fixed in 80% ethanol, changed twice, and later stored in 80% ethanol. DNA was extracted from whole specimens; tissues were hydrated in TE buffer (3 × 10 min), total genomic DNA was extracted with the SHERLOCK extraction kit (A&A Biotechnology), and the final product was dissolved in 20 μl of tris-EDTA (TE) buffer. The extracted DNA was stored at -80 °C at the Department of Malacology, Institute of Zoology and Biomedical Research, Jagiellonian University in Kraków (Poland).
DNA coding for mitochondrial cytochrome oxidase subunit I (COI) and nuclear histone 3 (H3) were sequenced. Details of PCR conditions, primers used, and sequencing are given in
Taxa used for phylogenetic analyses with their GenBank accession numbers and references.
Species | COI/H3 GB numbers | References |
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Agrafia wiktori Szarowska & Falniowski, 2011 | JF906762/MG543158 |
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Alzoniella finalina Giusti & Bodon, 1984 | AF367650 |
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Amnicola limosus (Say, 1817) | AF213348 |
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Anagastina zetavalis (Radoman, 1973) | EF070616 |
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Avenionia brevis berenguieri (Draparnaud, 1805) | AF367638 |
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Belgrandia thermalis (Linnaeus, 1767) | AF367648 |
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Belgrandiella kuesteri (Boeters, 1970) | MG551325/MG551366 |
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Bithynia tentaculata (Linnaeus, 1758) | AF367643 |
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Bythinella cretensis Schütt, 1980 | KT353689 |
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Bythinella hansboetersi Glöer & Pešić, 2006 | KT381101 |
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Bythiospeum acicula (Hartmann, 1821) | KU341350/ MK609536 |
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Bythiospeum alzense Boeters, 2001 | KU341355 |
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Ecrobia maritima (Milaschewitsch, 1916) | KX355835/MG551322 |
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Daphniola louisi Falniowski & Szarowska, 2000 | KM887915 |
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Dalmatinella fluviatilis Radoman, 1973 | KC344541 |
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Emmericia expansilabris Bourguignat, 1880 | KC810060 |
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Erhaia jianouensis (Y.-Y. Liu & W.-Z. Zhang, 1979) | AF367652 |
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Fissuria boui Boeters, 1981 | AF367654 |
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Graecoarganiella parnassiana Falniowski & Szarowska, 2011 | JN202352 |
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Graziana alpestris (Frauenfeld, 1863) | AF367641 |
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Grossuana angeltsekovi Glöer & Georgiev, 2009 | KU201090 |
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Hauffenia michleri (Kuščer, 1932) | KT236156/KY087878 |
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Heleobia maltzani (Westerlund, 1886) | KM213723/ MK609534 |
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Horatia klecakiana Bourguignat 1887 | KJ159128 |
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Hydrobia acuta (Draparnaud, 1805) | AF278808 |
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Iglica cf. gracilis (Clessin, 1882) | MH720985/ MH721003 |
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Iglica hellenica Falniowski & Sarbu, 2015 | KT825581/MH721007 |
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Islamia zermanica (Radoman, 1973) | KU662362/MG551320 |
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Kerkia jadertina (Kuščer, 1933) | KY087868/KY087885 |
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Kerkia jadertina sinjana (Kuščer, 1933) | KY087873-74/ KY087890-91 |
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Kerkia kareli Beran, Bodon & Cianfanelli, 2014 | KY087875-77/ KY087892-94 |
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Kerkia kusceri (Bole, 1961) | KY087867/KY087884 |
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Kerkia sp. Ljubač | KY087872/KY087889 |
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Littorina littorea (Linnaeus, 1758) | KF644330/KP113574 |
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Littorina plena Gould, 1849 | KF643257 |
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Lithoglyphus prasinus (Küster, 1852) | JX073651 |
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Marstoniopsis insubrica (Küster, 1853) | AF322408 |
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Moitessieria cf. puteana Coutagne, 1883 | AF367635/MH721012 |
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Montenegrospeum bogici (Pešić & Glöer, 2012) | KM875510/MG880218 |
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Paladilhiopsis grobbeni Kuščer, 1928 | MH720991/MH721014 |
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Peringia ulvae (Pennant, 1777) | AF118302 |
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Pomatiopsis lapidaria (Say, 1817) | AF367636 |
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Pontobelgrandiella sp. Radoman, 1978 | KU497024/MG551321 |
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Pseudamnicola chia (E. von Martens, 1889) | KT710656 |
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Pseudorientalia Radoman, 1973 – Lesvos | KJ920490 |
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Radomaniola curta (Küster, 1853) | KC011814 |
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Sadleriana fluminensis (Küster, 1853) | KF193067 |
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Sadleriana robici (Clessin, 1890) | KF193071 |
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Salenthydrobia ferrerii Wilke, 2003 | AF449213 |
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Sarajana apfelbecki (Brancsik, 1888) | MN031432 |
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Tanousia zrmanjae (Brusina, 1866) | KU041812 |
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Tricula sp. Benson, 1843 | AF253071 |
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Genus Kerkia Radoman, 1978
Ethanol-fixed specimen (Fig.
Twelve paratypes, ethanol-fixed, in the collection of the Department of Malacology of Jagiellonian University.
Shell minute, nearly planispiral, distinguished from K. kusceri by its lower aperture of the shell and smaller non-glandular outgrowth on the left side of the penis, and from K. jadertina and K. kareli by its higher aperture of the shell and bigger the non-glandular outgrowth on the left side of the penis.
Shell
(Fig.
Measurements
of holotype, sequenced, and illustrated shells: see Table
Shell measurements (in mm) of Kerkia briani. For explanation of the symbols A–H, see Fig.
A | B | C | D | E | F | G | H | |
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holotype | 0.77 | 1.39 | 0.87 | 0.62 | 0.60 | 1.09 | 0.41 | 0.97 |
2D44 | 0.72 | 1.12 | 0.73 | 0.54 | 0.55 | 0.93 | 0.37 | 0.75 |
2F59 | 0.73 | 1.26 | 0.82 | 0.54 | 0.55 | 0.95 | 0.41 | 0.80 |
2F62 | 0.72 | 1.35 | 0.86 | 0.57 | 0.57 | 1.03 | 0.36 | 0.73 |
2F70 | 0.67 | 1.12 | 0.72 | 0.52 | 0.48 | 0.97 | 0.40 | 0.72 |
2F71 | 0.75 | 1.37 | 0.85 | 0.46 | 0.60 | 1.02 | 0.41 | 0.84 |
M | 0.73 | 1.27 | 0.81 | 0.54 | 0.56 | 1.00 | 0.39 | 0.80 |
SD | 0.034 | 0.123 | 0.067 | 0.053 | 0.044 | 0.059 | 0.023 | 0.094 |
Min | 0.67 | 1.12 | 0.72 | 0.46 | 0.48 | 0.93 | 0.36 | 0.72 |
Max | 0.77 | 1.39 | 0.87 | 0.62 | 0.60 | 1.09 | 0.41 | 0.97 |
Soft parts morphology and anatomy.
Body white, without pigment, with no eyes. The ctenidium with twelve short lamellae, osphradium short and broad. Rectum forming characteristic broad loop (Fig.
Renal and pallial section of the female reproductive organs of Kerkia briani: A the loop of oviduct in its normal position and the loop of the rectum B the loop of oviduct moved to show the receptaculum seminis and duct of bursa. Abbreviations: bc – bursa copulatrix, cbc – duct of bursa, ga – albuminoid gland, gn – nidamental gland, gp – gonoporus, ov – oviduct, ovl – loop of renal oviduct, rec – rectum, rs – receptaculum seminis. Scale bars: 1 mm.
The specific epithet briani refers to our friend Brian Lewarne, Honorary Science Officer of The Devon Karst Research Society, and the Director for “Proteus Project in the Trebišnjica River Basin”, deeply devoted to the protection of Proteus as well as the study and protection of the subterranean habitats in Bosnia and Herzegovina.
Known from the type locality only.
We obtained six new sequences of COI (479 bp, GenBank Accession Numbers MT780191–MT780196), and six new sequences of H3 (309 bp, GenBank Accession Numbers MT786730–MT786735). The tests by
The sequences of the Kerkia briani formed distinct clade on COI, H3 as well as combined phylograms (Fig.
p-distances between Kerkia mOTUs for the COI (below diagonal) and H3 genes.
mOTU – A | mOTU – B | mOTU – C | mOTU – D | mOTU – E | |
---|---|---|---|---|---|
mOTU – A | – | 0.010 | 0.007 | 0.010 | 0.023 |
mOTU – B | 0.135 | – | 0.017 | 0.020 | 0.033 |
mOTU – C | 0.126 | 0.124 | – | 0.010 | 0.023 |
mOTU – D | 0.146 | 0.138 | 0.124 | – | 0.013 |
mOTU – E | 0.123 | 0.110 | 0.095 | 0.093 | – |
Following the terminology of
Finally, it has to be pointed out that K. briani inhabits the southernmost locality of Kerkia, expanding the range of the genus ca. 190 km ESE.
The study was supported by a grant from the National Science Centre 2017/25/B/NZ8/01372 to AF.