Research Article |
Corresponding author: Pongsak Laudee ( pongsak.l@psu.ac.th ) Academic editor: Ana Previšić
© 2020 Pongsak Laudee, Kriengkrai Seetapan, Chanda Vongsombath, Hans Malicky.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Laudee P, Seetapan K, Vongsombath C, Malicky H (2020) New species of caddisflies (Trichoptera, Ecnomidae, Polycentropodidae, Psychomyiidae) from Mekong tributaries, Laos. ZooKeys 962: 1-11. https://doi.org/10.3897/zookeys.962.52759
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Four new species of caddisflies are added to the fauna of Mekong River, Laos. Described and illustrated are Ecnomus petchanaae sp. nov. and E. boonsawaengae sp. nov. (Ecnomidae), Polyplectropus proukaewi sp. nov. (Polycentropodidae), and Psychomyia srichanai sp. nov. (Psychomyiidae) from tributaries of the Mekong River, Laos. Ecnomus petchanaae sp. nov. can be distinguished by the characters of the superior appendages, which are slender and club-shaped in lateral view. In E. boonsawaengae sp. nov., the inferior appendages are tubular with a concave incision subapically and truncated apex. Polyplectropus proukaewi sp. nov. has the distal process of the dorsal branch of the inferior appendage close to the base of the inferior appendages and the length of the process is half of inferior appendages. Psychomyia srichanai sp. nov. can be distinguished by the structure of the dorsal branches of the harpagones and apical sclerite. The outer branches of the harpago in the latter species are bifurcated and bent 90° degrees outward, and the inner dorsal branches of harpago are curved outward. The apical sclerite is indiscernible.
aquatic insects, biodiversity, Oriental Region, taxonomy
The Mekong River, with a length of 5,400 km is the 12th longest river in the world and passes through six countries, originating from China, through Myanmar, Laos, Thailand, Laos, Cambodia, and finally Vietnam (
Trichoptera (or caddiflies), among the holometabolous aquatic insects, are one of the largest groups of aquatic insects contributing to many aspects in an aquatic ecosystem as secondary consumers, tertiary consumers, or predators (
In this study, we focus on three caddisfly genera in Laos – Ecnomus McLachlan, Polycentropus Curtis, and Psychomyia Latreille. There are eight species of Ecnomus reported from Laos, including E. alkaios Malicky & Chantaramongkol, 1997, E. alkmene Malicky & Chantaramongkol, 1997, E. androgeos Malicky, 1997, E. amphitryon Malicky, 1997, E. volovicus Malicky & Chantaramongkol, 1993, E. caesar Malicky & Chaibu in
Considering the overall diversity of the Mekong River and its tributaries and the under-investigated caddisfly fauna of this region, many new species records and descriptions are expected. This article adds four new species from the Mekong River and its tributaries to the list of Trichoptera in Laos.
Adult caddisfly specimens were collected with a UV pan light trap (12 V, 10 W) operated along streams and the river overnight at the locations indicated below. Collected specimens were preserved in 70% ethanol, and caddisflies were later manually sorted from other insects. For species-level identifications, the male genitalia were observed under a stereomicroscope. For this purpose, the male genitalia from a specimen from each new species were dissected out. Muscle tissue was macerated by heating in 10% KOH at 60 °C for 30–60 minutes and then soaking in a detergent solution. Drawings were initially made in pencil using a compound microscope equipped with a drawing tube and used to produce the final vector graphics in Adobe Illustrator software.
Holotypes and paratypes are stored in 70% ethanol and deposited in Princess Maha Chakri Sirindhorn Natural History Museum, Prince of Songkla University, Hat Yai Campus, Hat Yai District, Songkhla Province, Thailand (
The male genitalia of E. petchanaae sp. nov. are similar to Ecnomus gapit Cartwright, 1994, E. yuleae Cartwright, 1994, E. dares Malicky, 2000, and E. perseis Malicky, 2008 described from Borneo. The superior appendages of all these species, including the new species, are particularly large and the subapical part of the superior appendages is covered by numerous spiny setae. However, E. petchanaae sp. nov. can be distinguished by the shape of its superior appendages, which, in lateral view, are slender and club-shaped, but basally broad in E. gapit, E. yuleae, E. dares, and E. perseis. In addition, in ventral view of the outer surface of the inferior appendages of the new species is crescent-shaped, whereas they are curved and claw-shaped in E. gapit, E. yuleae, E. dares, and E. perseis.
Ecnomus petchanaae, sp. nov. Male genitalia. A Segment IX and superior appendages, dorsal B segments IX and superior appendages, left lateral C segment IX and inferior appendages, ventral D phallus tip, ventral. Ter IX = tergum IX, Ste IX = sternum IX, Sup = superior appendage, Inf = inferior appendage.
Adult, male, length of each male forewing 5.6–6.0 mm; color in alcohol of head, thorax, forewings, abdomen, and legs brown. Male genitalia as in Figure
Holotype. Male. Laos: Pakse Province: Paksong, Vang Ngao River, 15°11'37"N, 106°06'40"E, elev. 920 m, 7.iv.2019, Pongsak Laudee. (
The species epithet honors Mrs Kanchanaluk Petchana, Director of Administration and Strategic Development Division, Prince of Songkla University, Surat Thani Campus.
The male genitalia of E. boonsawaengae sp. nov. are similar to E. aktaion Malicky & Chantaramongkol, 1997 and E. uttu Malicky & Chantaramongkol, 1993. In these species, the superior appendages are particularly long and slender with a basoventral process on the superior appendage. However, E. boonsawaengae sp. nov. can be easily distinguished by the shape of the inferior appendages. In lateral view, the inferior appendages are tubular with a subapical concave incision and truncated apex in E. boonsawaengae sp. nov., but in E. aktaion and E. uttu the inferior appendages are somewhat triangular and trapezoidal, respectively, and with a pointed apex. In addition, each inferior appendage in E. boonsawaengae sp. nov. has a process, in ventral view, which is lacking in E. aktaion and E. uttu.
Adult, male, length of each male forewing 4.0 mm; color in alcohol of head, thorax, forewings, abdomen, and legs grayish brown. Male genitalia as in Figure
Ecnomus boonsawaengae, sp. nov. Male genitalia. A Segment IX and superior appendages, dorsal B segment IX and superior appendages, left lateral C segment IX and inferior appendages, ventral D phallus, lateral. Ter IX = tergum IX, Ste IX = sternum IX, Sup = superior appendage, Inf=inferior appendage.
Holotype. Male. Laos: Pakse Province: Paksong, E-Tu Waterfall, 15°11'25"N, 106°06'14"E, elev. 900 m, 7.iv.2019, Pongsak Laudee. (
The species epithet honors Mrs Wasana Boonsawaeng, Vice Dean of Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus.
The male genitalia of P. proukaewi sp. nov. are similar to those of P. daimong Oláh & Johanson, 2010 from Vietnam. In both species, the dorsal branch of the inferior appendages forms a hooked-like process. However, P. proukaewi sp. nov. can be distinguished by the considerably shorter distance of the distal processes of the dorsal branch of the inferior appendages to their bases compared to P. daimong, in which this distance is considerably longer. Additionally, in the new species, the length of the distal processes in lateral view equals half of the length of inferior appendages, whereas the length of the distal processes equals the length of inferior appendages in P. daimong.
Adult, male, length of each male forewing 6.5–7 mm; color in alcohol of head, thorax, forewings, abdomen, and legs brown. Male genitalia as in Figure
Polyplectropus proukaewi, sp. nov. Male genitalia. A Segment X, dorsal B segments IX–X, left lateral C inferior appendages, ventral D inferior appendages, caudal. Ter IX = tergum IX, Ste IX = sternum IX, Ter X = Tergum X, Do Lat = dorsolateral processes of preanal appendages, Me Lat = mesolateral processes of preanal appendages, Me Ven = mesoventral processes of preanal appendages.
Holotype. Male. Laos: Pakse Province: Paksong, Vang Ngao River, 15°11'37"N, 106°06'40"E, elev. 920 m, 7.iv.2019, Pongsak Laudee. (
The species epithet honors Dr Nitat Proukaew, Assistant Professor in the Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus.
The male genitalia of Ps. srichanai sp. nov. are similar to those of three other Psychomyia species described from Thailand, Ps. amor Malicky & Chantaramongkol, 1997, Ps. amphiaraos Malicky & Chantaramongkol, 1997 and Ps. monto Malicky & Chantaramongkol, 1993, as well as of Ps. sonlana Oláh & Malicky, 2010 from Vietnam. Differences are mainly seen in the structure of the dorsal branches of the harpagones and apical sclerite. The dorsal branch of each harpago in Ps. srichanai sp. nov. is divided into two branches. The outer branch curves downward and bifurcates apically, whereas the inner branch is long, curved upward, and apically pointed. In Ps. amphiaraos and Ps. monto, the outer dorsal branch does not bifurcate. The outer dorsal branches of the harpagones are also bifurcated in Ps. amor and Ps. sonlana. However, only in Ps. srichanai sp. nov. are they are bent outward at 90°. The apical sclerite is apically pointed and discernable in Ps. amphiaraos, Ps. monto, Ps. amor, and Ps. sonlana, but indiscernible in Ps. srichanai sp. nov.
Length of each male forewing 3.0–4.0 mm; color in alcohol of head, thorax, forewings, abdomen, and legs yellow brown. Male genitalia as in Figure
Holotype. Male. Laos: Luang Prabang Province: Elephant Camp, Mekong River, 20°01'46"N, 102°13'13"E, elev. 280 m, 2.iii.2019, Pongsak Laudee. (
The species epithet honors Prof. Dr Teerapol Srichana, Director of the Research and Development Office, Prince of Songkla University, Hat Yai Campus.
Ecnomus petchanaae sp. nov., E. boonsawaengae sp. nov. and P. proukaewi sp. nov. were collected from a stream and waterfall on Bolaven Plateau, southern Laos. Here, forest type is montane evergreen rainforest. The three new species are rhithral species, which live in small streams where the substrate is dominated by boulders and cobblestones (Fig.
This research is supported by the Thailand Research Fund (TRF), project number DBG 6180021. We thank Assoc. Prof. Dr Seppo Karrila for comments and linguistic assistance. We also thank Dr Manpreet Singh Pandher and an anonymous reviewer for providing very useful suggestions to improve the manuscript.