Research Article |
Corresponding author: Nasreen Peer ( peer.nasreen@gmail.com ) Academic editor: Sammy De Grave
© 2015 Nasreen Peer, Renzo Perissinotto, Gavin Gouws, Nelson A.F. Miranda.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Peer N, Perissinotto R, Gouws G, Miranda NAF (2015) Description of a new species of Potamonautes MacLeay, 1838, from the iSimangaliso Wetland Park, South Africa. ZooKeys 503: 23-43. https://doi.org/10.3897/zookeys.503.9532
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A new species of freshwater crab, Potamonautes isimangaliso sp. n., is described from the western shores of False Bay, Hluhluwe, within the iSimangaliso Wetland Park, South Africa. While bearing a superficial resemblance to P. lividus, the new species has been found to be genetically distinct, diverging from the former by 7.4–7.8% in mtDNA. Potamonautes isimangaliso most closely resembles P. lividus, but is distinguished by a unique suite of carapace characters, colouration, and size. The new species also lives in close association with oxygen-poor, fresh ephemeral pans, while the habitat of P. lividus is well above the surface water line of the closest water body. An updated identification key for the Potamonautes species of South Africa is provided.
Brachyura , freshwater, Potamonautes , taxonomy, ephemeral pans, sand forest, iSimangaliso Wetland Park
Freshwater crabs play a key role in ecosystems by serving as an important food source for larger taxa, and recycling nutrients through detritivorous feeding habits (
The iSimangaliso Wetland Park forms the southernmost region of the Maputaland centre of endemism and constitutes the focus of biodiversity conservation in the region (
Map of Lake St Lucia on the east coast of South Africa. Collection localities, indicated by black dots and labelled with codes, are all restricted to the western shore of False Bay, within the False Bay Park. FB1 = Main Road Pan; FB3 = Mpophomeni Pan; FB5 = Dukandlovu Pan; FB6 = Sandy Point Pan.
In this paper we describe Potamonautes isimangaliso sp. n. from the sand forests of the iSimangaliso Wetland Park. NP and GG wrote the taxonomic part of this study, including the description of the new species, while the contribution of the other authors dealt with natural history and ecological observations.
Crabs were collected from four localities (Fig.
In the laboratory, a pair of Vernier callipers was used to measure morphological variables. A Nikon SMZ25 microscope fitted with a Nikon Digital Sight DS-Fi2 camera was used for macro-examination and to take photos of gonopods and mouthparts.
Abbreviations for depositories and provinces:
ISAM Iziko South African Museum, Cape Town, South Africa
NMMU Nelson Mandela Metropolitan University Collection.
EC Eastern Cape
WC Western Cape
KZN KwaZulu–Natal
Abbreviations for all morphological and morphometric characters (
CL Carapace length
CWW Carapace widest width
CWP Carapace posterior width
PFCD Distance between postfrontal crest and anterior margin
ED Distance between orbits
CWA Distance between exorbital teeth
CH Carapace height
AW6 Width of sixth abdominal segment
MCPL Major cheliped propodus length
MCPH Major cheliped propodus height
P2ML Pereopod 2, merus length
P2MH Pereopod 2, merus height
s2/s3 First sternal groove (suture between the second and third sulci)
s3/s4 Second sternal groove (suture between the third and fourth sulci)
CRDL Right cheliped, dactyl length
CLDL Left cheliped, dactyl length
CRPL Right cheliped, propodus length
CLPL Left cheliped, propodus length
CRPW Right cheliped, propodus width
CLPW Left cheliped, propodus width
ML Merus length
MW Merus width
For the morphometric analyses, eight variables (CL, PFCD, CWP, ED, CH, AW6, CRPL and CRPW) were log transformed and used to run a stepwise discriminant function analysis in STATISTICA v 12.5 (Statsoft 2004). Data for Potamonautes lividus were obtained from G. Gouws and represent the specimens used for the description of P. lividus (
DNA was extracted from each specimen and amplification of the mitochondrial cytochrome c oxidase subunit I (COI) and 16S ribosomal DNA genes were carried out following protocols outlined by G. Gouws (unpubl.). Amplifications were confirmed by electrophoresis in 1% agarose gels with an ethidium bromide stain. The product was then viewed on an ultraviolet transilluminator. Sequences were generated from a representative of the new species, using approaches described elsewhere (
The new species (Potamonautes isimangaliso sp. n.) was distinguished from P. lividus by its larger size, flatter carapace and more rounded posterior. The carapace variables CL, CH and CWP contributed the most to distinguishing between the two forms in the discriminant analysis. Fig.
The classification function was calculated for both species as follows:
Y(P. isimangaliso) = 926.798(LogCL) – 602.076(LogCH) – 7.966(LogCWP) – 178.319
and
Y(P. lividus) = 1428.33(LogCL) – 743.234(LogCH) – 321.805(LogCWP) – 296.179
Individuals were reassigned to groups based on a priori probabilities using these classification functions. 100% of both forms were correctly classified with no individuals being reassigned. Three variables (CWP, PFCD and CH) were regressed over CL and a significant difference was seen between the two species as follows: CWP/CL – SS = 0.1, df = 2, F = 2.29, p < 0.001; PFCD/CL – SS = 0.1, df = 2, F = 2.29, p < 0.001; CH/CL – SS = 0.1, df = 2, F = 5.99, p < 0.001 (Fig.
Holotype: male, CL = 37 mm, ephemeral pan 200 m away from the western fence of False Bay Park (FB3), iSimangaliso Wetland Park (27°57'31.33"S, 32°21'42.15"E; elevation 62 m), 2 February 2015, R. Perissinotto, R.H. Taylor, D. Bilton, M.S. Bird, S.J. du Plooy and L. Clennell legit (ISAM A78908).
Allotype: female, CL = 27 mm, ephemeral pan, next to road leading from Dukandlovu campsite to False Bay Park entrance gate (FB5), 5 km south of Lister’s Point, iSimangaliso Wetland Park (28°0'51.70"S, 32°21'55.36"E; elevation 10 m), 1 February 2015, R. Perissinotto, R.H. Taylor, D. Bilton, M.S. Bird, S.J. du Plooy and L. Clennell legit (ISAM A78909).
Paratypes: one male, one female, collection data same as per holotype (NMMU); one male, ephemeral pan along the main road of False Bay Park (FB1), iSimangaliso Wetland Park (27°58'32.02"S, 32°21'51.62"E; elevation 42 m), 1 February 2015, R. Perissinotto, R.H. Taylor, D. Bilton, M.S. Bird, S.J. du Plooy, and L. Clennell legit (ISAM A78910); one male, ephemeral pan, collection data same as per allotype (ISAM A78911); two males, two females, ephemeral pan along the main road of False Bay Park (FB1), iSimangaliso Wetland Park (27°58'32.02"S, 32°21'51.62"E; elevation 42 m), 31 January 2015, R. Perissinotto, R.H. Taylor, D. Bilton, M.S. Bird, S.J. du Plooy, and L. Clennell legit (ISAM A78912); two females, collection data same as per holotype, 26 November 2013, R. Perissinotto, R.H. Taylor, N. Peer, N.A.F. Miranda, M.S. Bird, J.L. Raw and L. Clennell legit (NMMU); one male, one female, ephemeral pan near Sandy Point in False Bay Park (FB 6), iSimangaliso Wetland Park (27°58'36.0"S, 32°22'17.0"E; elevation 12 m), 25 November 2013, R. Perissinotto, R.H. Taylor, N. Peer, N.A.F. Miranda, M.S. Bird, J.L. Raw and L. Clennell legit (ISAM A78913); two males, ephemeral pan, collection data same as per allotype, 5 December 2012, R. Perissinotto, N.A.F. Miranda, N. Peer, J.L. Raw legit (ISAM A78914).
Main distinguishing features of P. isimangaliso from P. lividus Gouws, Stewart & Reavell, 2001 as follows: slightly granulated, horizontal anterolateral margin more rounded than in P. lividus; downward projection of postfrontal crest at exorbital edges; uniform colouration of dark purplish brown with lighter or orange coloured joints, cheliped tips and pereopods tips. Potamonautes isimangaliso is larger than P. lividus, with a maximum size of 37 mm CL recorded in males.
Carapace (Fig.
Sternites (Fig.
Third maxillipeds (Figs
Potamonautes isimangaliso sp. n. male holotype CWW 55.1 mm (ISAM A78908). a major cheliped b minor cheliped c right mandibular palp posterior view d right mandibular palp anterior view e 3rd maxilliped f left gonopod 1 anterior view with enlarged terminal segment g left gonopod 1 posterior view with enlarged terminal segment h left gonopod 2 anterior view and i left gonopod 2 posterior view. Scale bars: 10 mm (a, b), 1 mm (c, d), 10 mm (e), 5 mm (f, g), 1 mm (h, i). Photos: Nasreen Peer.
Mandibular palp (Fig.
Pereopods (Figs
Pleon (Fig.
Pleopods (Fig.
Variation. The major cheliped does not always display a pronounced interspace when fingers are closed. In juveniles and in the female allotype this was less prominent. The arching of chelipeds varies too, with some (particularly the minor chelipeds) bearing straight dactyli while others are fairly rounded. All collected specimens display a pronounced heterochely and all appear to be right-handed.
Morphometric variables (mm) of Potamonautes isimangaliso sp. n. holotype and paratype specimens.
Variable | Holotype | Males (n=8) | Females (n=7) |
---|---|---|---|
CL | 37 | 13.2–36.1 | 18–27 |
CWW | 55.1 | 18–53 | 26–40.1 |
CPW | 24 | 9–24 | 10.5–20 |
PFCD | 6 | 2–5 | 2.8–4.1 |
ED | 15.6 | 5–16 | 8.5–12.8 |
CWA | 34 | 14.1–40.5 | 21.5–32 |
CH | 21 | 7–23.2 | 11–12.1 |
AW6 | 12 | 3.1–11 | 7–23.4 |
MCPL | 49.3 | 11.5–44.5 | 17.2–29.2 |
MCPH | 21.9 | 4.1–20.1 | 7–13.7 |
P2ML | 21.3 | 6.5–18.9 | 8.9–13.8 |
P2MH | 6.5 | 2–6.1 | 3–5 |
Colouration of carapace may vary between light brown, maroon, purplish-brown and almost black. Similarly, tips of dactyli may be either orange, bright yellow or a dull yellow.
Currently only known from the False Bay region of the iSimangaliso Wetland Park on the north-east coast of South Africa.
South Africa, KwaZulu-Natal, iSimangaliso Wetland Park, False Bay - Western Shores: Mpophomeni Pan (27°57'31.33"S, 32°21'42.15"E); Dukandlovu Pan (28°0'51.70"S, 32°21'55.36"E); Main Road Pan (27°58'32.02"S, 32°21'51.62"E); Sandy Point Pan (27°58'36.0"S, 32°22'17.0"E).
The species is named after the iSimangaliso Wetland Park, located in northern KwaZulu-Natal, where it is currently thought to be micro-endemic. This is significant as the iSimangaliso Wetland Park falls within the Maputaland centre of endemism (
Potamonautes isimangaliso sp. n. is easily distinguishable from most other Potamonautes spp. found in South Africa. Potamonautes dentatus Stewart, Coke & Cook, 1995, P. parvispina Stewart, 1997, P. unispinus Stewart & Cook, 1998, P. warreni Calman, 1918 and P. calcaratus (Gordon, 1929) all bear dentate anterolateral margins or epibranchial corners, while P. isimangaliso has a rounded epibranchial corner and mildly granular anterolateral margin.
Potamonautes perlatus (H. Milne Edwards, 1837), P. granularis Daniels, Stewart & Gibbons, 1998, P. sidneyi Rathbun, 1904, P. barbarai Phiri & Daniels, 2014 and P. barnardi Phiri & Daniels, 2014 all have sharply-defined scabrous or granular epibranchial corners and prominent postfrontal crest, while P. isimangaliso has a heavily rounded smooth epibranchial corner and poorly-defined postfrontal crest. Potamonautes parvicorpus Daniels, Stewart & Burmeister, 2001 also displays a finely granulated anterolateral margin and rounded epibranchial corners, but the resemblance to P. isimangaliso is superficial, as it differs in the indentation of its anterior margin where P. parvicorpus bears a slightly indented anterior margin while that of P. isimangaliso lies straight. Further differences are seen in locality as the habitat of P. parvicorpus is restricted to high mountain streams in the Western Cape (
Potamonautes clarus Gouws, Stewart & Coke, 2000, P. depressus (Krauss, 1843), P. brincki (Bott, 1960), P. flavusjo Daniels, Phiri & Bayliss, 2014 and P. lividus Gouws, Stewart & Reavell, 2001 all have smooth anterolateral margins and rounded smooth epibranchial corners but bear differences compared to P. isimangaliso. One of the diagnostic characters of P. depressus is the dorsally flattened carapace, where CL/CH = 2.3–2.6. Potamonautes isimangaliso has a more vaulted carapace with a CL/CH ratio of 1.6–1.8. Potamonautes brincki and P. clarus are smaller crabs (max CL = 27 mm in males for both species), preferring fast-flowing mountain stream habitats. Potamonautes flavusjo is ecologically distinct from P. isimangaliso and can be found in the Mpumalanga Highveld. In addition to this, the species is smaller and has flattened chelipeds, not adapted for burrowing (
Potamonautes lividus shares a similar distribution, outward appearance and preference for air-breathing with P. isimangaliso. However various differences exist between the two species.The level and angle of anterolateral margin differ, where P. isimangaliso bears an anterolateral margin lying on the same horizontal plane as the anterior margin. Conversely, P. lividus has an anterolateral margin which angles downward to join the anterior margin and thus sits higher than the anterior margin. The downward angle of postfrontal crest at exorbital edges is seen in P. isimangaliso but not in P. lividus. Carapace flatness is indicated by the CL/CH ratio which equates to 1.5 for P. lividus and 1.8 for P. isimangaliso holotypes. The maximum size (37 mm CL in P. isimangaliso and 25.5 mm CL in P. lividus), colouration (dark blue carapace with bright orange chelipeds in P. lividus and dark brown/purple carapace with brown or dull yellow cheliped in P. isimangaliso), inflation of chela with gap between propodus and dactyl (dactyl of P. lividus is more arched than that of P. isimangaliso) and the number of poorly-developed teeth on carpus (P. lividus containing one prominent and three rudimentary teeth; P. isimangaliso containing one prominent and one rudimentary tooth) further distinguish the two species. Gonopods of both species are very similar with the only difference being the tuft of setae found at the base of pleopod 2 in P. isimangaliso. Specimens resembling P. lividus were found in the Dwesa Forest, Eastern Cape and appear to be genetically nearly identical to P. lividus (
Preliminary sequence data for two mitochondrial gene regions (16S: GenBank accession number KR137640; COI: KR137642) generated from a representative of the new species, using approaches described elsewhere (
Potamonautes isimangaliso sp. n. inhabits freshwater ephemeral pans (maximum salinity recorded: 0.75) which fill up with fresh, oxygen-deprived water after rainfall events, mainly during the summer wet season. These pans are located along the western shores of False Bay, Lake St Lucia (Fig.
Potamonautes isimangaliso adults form burrows on the banks of these pans (Fig.
Main Road Pan (FB1) | Mpophomeni Stream (FB2) | Mpophomeni Pan (FB3) | Dukandlovu Pan (FB5) | |
---|---|---|---|---|
Temperature (°C) | 26.1 | 20.4 | 22.03 | 26.73 |
Salinity | 0.15 | 8.36 | 0.29 | 0.75 |
Maximum depth (mm) | 80 | 500 | 700 | 250 |
pH | 7.2 | 7.03 | 7.42 | 6.9 |
Turbidity (NTU) | 1310.5 | 14 | 151 | 306.3 |
Dissolved oxygen (% sat.) | 19.8 | 90.1 | 22.4 | 69.6 |
Although the species appears to be more closely associated with water than its morphologically closest congener, P. lividus (
The feeding ecology of the species is largely unknown, although Potamonautes crabs are generally thought to shift from a diet of aquatic invertebrates to a more herbivorous or opportunistic diet with age (
Based on
1 | Anterolateral margin bearing one tooth or many distinct teeth | 2 |
– | Anterolateral margin smooth tooth, sometimes serrated or granulated | 6 |
2 | Anterolateral margin bearing two or more distinct teeth | 3 |
– | Anterolateral margin bearing one distinct tooth at epibranchial corner | 4 |
3 | Postfrontal crest complete to anterolateral margin; epibranchial sinus absent | Potamonautes warreni Calman, 1918 |
– | Postfrontal crest interrupted at exorbital teeth; epibranchial sinus present | P. dentatus Stewart, Coke & Cook, 1995 |
4 | Postfrontal crest not continuous posterior to exorbital teeth; merus of chelipeds bearing a spine on both antero- and posterior-inferior granulate margins | P. calcaratus (Gordon, 1992) |
– | Postfrontal crest complete; no spine on merus of cheliped | 5 |
5 | Postfrontal crest not sloping backwards towards anterolateral margin; epibranchial sinus absent | P. unispinus Stewart & Cook, 1998 |
– | Postfrontal crest sloping backwards to meet anterolateral margin; epibranchial sinus present | P. parvispina Stewart, 1997 |
6 | Anterolateral margin granulated, forming distinct angle with postfrontal crest at epibranchial corner; epibranchial region usually scabrous or granulated | 7 |
– | Anterolateral margin rounded and smooth, meeting postfrontal crest at rounded epibranchial corner; epibranchial region usually without scabrosity or granulation | 10 |
7 | Carapace and postfrontal crest strongly granulated, with pronounced scabrosity in epibranchial region | 8 |
– | Carapace and postfrontal crest moderately granulated, with relatively smooth epibranchial region | 9 |
8 | Carapace anterior relatively narrow and curved moderately over the branchial region; inward-extending lobe absent from short terminal segment of gonopod 2; confined to the Olifants River system in the Cape Fold Mountains below the Bulshoek dam wall (WC) | P. granularis Daniels, Stewart & Gibbons, 1998 |
– | Carapace anterior relatively wide and curved slightly over the branchial region; long slender S-shaped terminal segment of gonopod 2 is formed by inward extending lobe; not occurring in the Western Cape | P. sidneyi Rathbun, 1904 |
9 | Occurring largely in the Western Cape, also extending further north and east; found in western flowing drainage systems including the Olifants River, where it occurs above the Bulshoek dam wall | P. perlatus (H. Milne Edwards, 1837) |
– | Restricted to southern flowing drainages in the Western Cape (Gamtoos River and Gourits River) | P. barbarai Phiri & Daniels, 2014 |
– | Restricted to the Berg River and tributaries of the Breede River (WC) | P. barnardi Phiri & Daniels, 2014 |
10 | Carapace depressed and dorso-ventrally flattened; ratio of carapace length to height between 2.1 and 2.6 | 11 |
– | Carapace vaulted and arched; ratio of carapace length to height between 1.5 and 2.2 | 12 |
11 | Carapace flatter, with a ratio of carapace length to carapace height between 2.3 and 2.6; postfrontal crest often directed forward near anterolateral margin; dark yellow-brown or green-brown in colour | P. depressus (Krauss, 1843) |
– | Carapace more vaulted, with ratio of carapace length to carapace height between 2.1 and 2.3; postfrontal crest straight near the anterolateral margin; orange in colour | P. clarus Gouws, Stewart & Coke, 1995 |
12 | Dactyli of chelipeds flattened; postfrontal crest, pereopods and chelipeds bright yellow; occurring in the Mpumalanga Highveld | P. flavusjo Daniels, Phiri & Bayliss, 2014 |
– | Dactyl of chelipeds moderately or highly arched | 13 |
13 | Anterolateral margin curving inward over carapace surface in the branchial region; occurring in KZN | 14 |
– | Anterolateral margin not curving inward over carapace surface in the branchial region; occurring in the WC | 15 |
14 | Bearing one prominent tooth and three rudimentary teeth on carpus of cheliped; tuft of setae absent from base of pleopod 2; carapace dark with distinctive blue sheen; chelipeds and pereopods bright orange | P. lividus Gouws, Stewart & Reavell, 2001 |
– | Bearing one prominent tooth and one rudimentary tooth on carpus of cheliped; tuft of setae present at base of pleopod 2; carapace uniformly purplish brown with similar coloured or slightly brighter chelipeds and pereopods | P. isimangaliso sp. n. |
15 | Flange present on terminal segment of mandibular palp | P. brincki (Bott, 1960) |
– | Flange absent from terminal segment of mandibular palp | P. parvicorpus Daniels, Stewart & Burmeister, 2001 |
The authors are grateful to R.H. Taylor, L. Clennell, D. Bilton, M.S. Bird, S.J. du Plooy and J.L. Raw for assistance with collections. L. Clennell is acknowledged for photography. P-P. Steyn and J.B Adams are thanked for their help with plant identification. The iSimangaliso Wetland Park Authority and Ezemvelo KZN Wildlife are also gratefully acknowledged for providing logistical assistance and permits for this project. We are grateful to Thobile Ndlovu for providing rainfall data for the area. This work is based on the research supported by the South African Research Chairs Initiative of the Department of Science and Technology (DST) and National Research Foundation (NRF) of South Africa. Any opinion, finding and conclusion or recommendation expressed in this material is that of the author(s) and the NRF does not accept any liability in this regard.