Research Article |
Corresponding author: Jimin Lee ( leejm@kiost.ac.kr ) Academic editor: Danielle Defaye
© 2020 Jong Guk Kim, Jimin Lee.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Kim JG, lee J (2020) A new genus and species of Nannopodidae (Crustacea, Copepoda, Harpacticoida) from the Yellow Sea of South Korea. ZooKeys 984: 23-47. https://doi.org/10.3897/zookeys.984.52252
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A new monospecific genus of the family Nannopodidae Brady, 1880 is proposed, based on specimens of both sexes of Concilicoxa hispida gen. et sp. nov. collected from subtidal sandy sediments in the Yellow Sea of South Korea. The presence of a coxal outer projection on the first to fourth legs and reduction of both rami of the second to fourth legs in this new genus show a clear relationship with a clade, which is characterised by the modified thoracopods for burrowing ability, comprising Huntemannia Poppe, 1884, Rosacletodes Wells, 1985, Laophontisochra George, 2002, Acuticoxa Huys & Kihara, 2010 and Talpacoxa Corgosinho, 2012 in Nannopodidae. Within this clade, C. hispida gen. et sp. nov. is most closely related to L. maryamae George, 2002 in having the prehensile endopod in the first leg, broad intercoxal sclerite on the second to fourth legs and the female fifth leg being composed of separate exopod and baseoendopod, but is distinguished by the absence of mandibular exopod, two-segmented mandibular endopod, presence of four setae on the distal exopodal segment of the first leg, and fusion of the intercoxal sclerite to the coxae in the third and fourth legs. These four features are considered as autapomorphies of the new genus. The possible relationship amongst members of the nannopodid clade is further discussed. Additionally, some comments on the taxonomic position of L. terueae Björnberg, 2014 are given, resulting in the transfer of the species to Acuticoxa as A. terueae comb. nov.
benthic copepod, Concilicoxa hispida gen. et sp. nov., East Asia, meiofauna, taxonomy
Recently,
In the present study, a new genus, attributed here to the family Nannopodidae, is proposed to accommodate a new harpacticoid collected from subtidal sandy sediments around the Socheongcho Ocean Research Station (SORS), which is a platform-type observation tower in the Yellow Sea of South Korea. SORS plays an important role in monitoring ocean and meteorological changes related to global climate change. Herein, we describe this new taxon and clarify its taxonomic relationship within Nannopodidae. Additionally, we also discuss the taxonomic position of L. terueae Björnberg, 2014.
Sampling for meiofauna was carried out from off SORS in the Yellow Sea of South Korea (Fig.
We adopted the descriptive terminology of
ae aesthetasc;
P1–P6 first to sixth thoracopod;
exp(enp)-1(-2, -3) to denote the proximal (middle, distal) segment of exopod (endopod).
Prior to scanning electron micrography (SEM), specimens were pre-fixed with 2.5% glutaraldehyde for 4 h, post-fixed with 2% osmium tetroxide for 2 h and then stored in 0.1 M phosphate buffer (pH 7.4) overnight. At each step, the samples were washed with phosphate buffer solution three times for 10 min each. The materials were dehydrated through a graded series of ethanol dilutions (50%, 60%, 70%, 80%, 90%, 100%) for 30 min each, dried in a freeze dryer (Hitachi ES-2030; Japan), coated with gold in an evaporator (Hitachi E-1045; Japan) and then examined via SEM (Hitachi S-4300; Japan).
Type materials were deposited in the Marine Interstitial fauna Resources Bank (MInRB) of the Korea Institute of Ocean Science and Technology (KIOST), Busan, South Korea.
Nannopodidae. Body subcylindrical, slightly depressed dorsoventrally, without distinct constriction between prosome and urosome; hyaline frills of somites weak, ornamented with long setules. Rostrum well-developed, triangular, with 1 pair of sensilla. Genital slit ♀ reverse U-shaped, covered by 1 pair of large opercula. Caudal rami elongate, oval in ♀, rectangular in ♂, with 7 setae; principal seta V as long as caudal rami in ♀, slightly shorter than urosome in ♂. Antennule 4-segmented, with elongate first segment in ♀; chirocerate and 6-segmented in ♂. Antennary allobasis without abexopodal seta; exopod represented by single seta. Mandibular palp uniramous, with 1 seta on basis; exopod absent and endopod 2-segmented. Maxillular praecoxal arthrite with 1 surface seta; exopod 1-segmented and endopod absorbed into basis. Maxillary syncoxa with 2 endites; endopod absorbed into basis. Maxilliped with elongate basis; endopod drawn out into long geniculate claw. P1 coxa with 1 coarsely serrate outer projection; inner element on basis displaced onto anterior surface; exopod 2-segmented, with 4 setae on exp-2; endopod arising from well-developed inner pedestal of basis, prehensile, 2-segmented; enp-1 elongate, unarmed; enp-2 with 1 stout claw and 1 long seta. P2–P4 with 1 coarsely serrate outer projection on coxa; intercoxal sclerite hugely broad, separated in P2 and laterally fused to coxae in P3 and P4; outer setophore on basis articulated in P3 and P4; exopod 1-segmented; proximal outer spine on exopod with serrate outer margin, with inner and outer longitudinal rows of setules; endopod absent in P2, represented by 1 small distinct protuberance in P3, 1-segmented in P4; male P3 endopod 1-segmented and armed with 1 stout spine. Setal armature formulae of P1–P4 as follows:
P5 baseoendopod broad; endopodal lobe weakly developed, with 1 seta; exopod 1-segmented, with 4 setae.
P6 represented by 2 setae in ♀; slightly asymmetrical and represented by 3 setae in ♂.
Concilicoxa hispida gen. et sp. nov., by monotypy.
The generic epithet is a combination of the Latin verb concílĭo meaning ‘unite separate parts into a whole’ and the Latin noun coxa, meaning ‘hip’ and alludes to the fusion of the coxae and the intercoxal sclerite in P3 and P4. It is a noun in the feminine singular.
Off the Socheongcho Ocean Research Station (SORS) (37°25'57.16"N, 124°44'56.4"E) in the Yellow Sea of South Korea, sandy sediments, 68 m depth.
Holotype : SOUTH KOREA•♀ dissected and mounted on 11 slides; the Yellow Sea, off SORS; 37°25'57.16"N, 124°44'56.4"E; 68 m depth; 23 Mar 2018; Kim, J.G. leg.; sandy sediments; cat. MInRB-Hr59-S001.
Allotype : SOUTH KOREA•♂ dissected and mounted on 11 slides; same data as for holotype; cat. MInRB-Hr59-S002.
Paratypes : SOUTH KOREA•3♀♀2♂♂ dissected and mounted on 11 or 12 slides each; same data as for holotype; cat. MInRB-Hr59-S003–MInRB-Hr59-S007•3♀♀2♂♂ preserved together in 95% ethanol; same data as for holotype; cat. MInRB-Hr59-L001.
SOUTH KOREA•2♀♀1♂ on a stub for SEM; same data as for holotype.
(MInRB-Hr59-S001). Total body length 617 μm (measurement based on holotype and six paratypes: range = 530–626 μm; mean = 588 μm; n = 7); maximum width 86 μm measured at the middle of cephalothorax. Body (Figs
Caudal rami (Figs
Antennule (Fig.
Antenna (Fig.
Mandibular coxa (Fig.
Maxillule (Fig.
Maxilla (Fig.
Maxilliped (Fig.
P1 (Fig.
P2–P4 (Figs
P5 (Fig.
Male
(allotype MInRB-Hr59-S002). Total body length slightly shorter than in female, 525 μm (measurement based on allotype and 4 paratypes: range = 485–556 μm; mean = 512 μm, n = 5); body (Fig.
Antennule (Fig.
P3 (Fig.
P5 (Figs
P6 (Figs
Spermatophore as long as 4/5 length of P5-bearing and genital somites combined (Figs
The species epithet “hispida” is derived from the Latin adjective híspĭdus, which means ‘hairy’ and refers to the setulose lateral ornamentation of the anal somite and caudal rami in the female. It is a noun in the feminine singular.
The investigated individuals of Concilicoxa hispida gen. et sp. nov. show intraspecific differences in appendage ornamentation. Dense spinular ornamentation was observed on the mandibular basis in one female paratype (MInRB-Hr-59-S003). This paratype also displays fusion of the coxa and basis of the P2 symmetrically (Fig.
The new genus Concilicoxa gen. nov. is assigned to the Nannopodidae because, as a member of the nannopodid clade, it exhibits the burrowing adaptation of the thoracopods. Concilicoxa gen. nov. appears to be closely related to both Laophontisochra and Acuticoxa in that they share four-segmented female antennules with elongate first segments, the prehensile P1 endopod, the presence of coxal outer projection on the P1, large and broad intercoxal sclerites on the P2–P4, the general shape of the female genital field (with a large copulatory pore and a well-developed operculum derived from P6) and elongate caudal rami. However, the novel genus is easily distinguishable from Laophontisochra by the distal armature of the antennary endopod with three geniculate and three non-geniculate elements (vs. four geniculate and two non-geniculate elements in Laophontisochra), the presence of coxal outer projections in the P2–P4 (vs. absent in Laophontisochra) and one-segmented exopods in the P2–P4 (vs. two-segmented in Laophontisochra). The new genus is also different from Acuticoxa in the absence of the P2 endopod (vs. one-segmented in Acuticoxa), a serrate coxal outer projection in the P1–P4 (vs. acute in Acuticoxa) and the female P5 exopod and baseoendopod separate (vs. fused into a single plate in Acuticoxa).
In contrast to a close resemblance with both genera in habitus and thoracopod morphology, Concilicoxa gen. nov. displays unambiguous autapomorphies that require the formation of a new genus: (1) the loss of the mandibular exopod, as observed in Huntemannia, is more derived than the exopod represented by a single seta; (2) the mandibular endopod is two-segmented, which seems to be secondarily divided, comparing to other related genera with only one-segmented endopod; (3) the P1 exp-2 comprises a total of only four elements, but five or six setae in Laophontisochra and Acuticoxa, respectively (in the original description of L. terueae, this segment was described as having one lateral and three terminal setae, but was depicted as having three outer and three terminal elements; see
The males of Concilicoxa gen. nov. exhibit distinctive potential autapomorphies for the genus as follows: (1) the P3 endopod has a sexual dimorphic distal element that is a robust spine; (2) the shape of P5 is nearly similar to that of the female; and (3) the caudal rami show sexual dimorphisms in the length of caudal seta V, the issuing position of setae I and II and the number of tube pores. However, we could not compare these characters with other related genera, because males of L. maryamae and A. ubatubaensis remain unknown. The sexual dimorphism of thoracopods is one of the most robust characters used to assess the phylogenic relationships between genera and between families because it facilitates comparison of the positions of homologue elements (such as setae or apophyses) of rami in females and males (
Harpacticoids generally display sexual dimorphism in the size, shape and setae of the male P5. However, no sexual dimorphism has been observed in the male P5 of Arenopontiidae Martínez Arbizu & Moura, 1994 (
The monophyly of the family Nannopodidae has been questioned by several researchers (e.g.
As reported by
Although the male of Laophontisochra remains unknown, a derived condition may be expressed in the male antennules–with a single compound segment distal to geniculation in A. terueae comb. nov. and C. hispida gen. et sp. nov. The male antennule of T. brandini possesses two segments distal to geniculation and the male antennule of R. kuehnemanni (Pallares, 1982) remains undescribed.
Given these characteristics, the clade with outer coxal projections on the P1–P4 can be subdivided into three groups: (1) Huntemannia, which is characterised by the presence of a setular group on the rostrum, a five-segmented female antennule, the absence of geniculate setae on the distal armature of the antennary endopod, a one-segmented antennary exopod with four setae, a one-segmented mandibular palp, non-prehensile P1 endopod, sexual dimorphism expressed in the number of elements on the distal segment of the male P3 endopod; (2) Rosacletodes and Talpacoxa, which are characterised by the absence of a setular group on the rostrum, a five-segmented female antennule, the presence of geniculate setae on the distal armature of the antennary endopod, a one-segmented antennary exopod with three setae, a two-segmented mandibular palp, prehensile short P1 endopod, P1 exopod with stout spines and presence of a sexually-dimorphic apophysis on the distal endopodal segment of the male P3; and (3) Laophontisochra, Acuticoxa and Concilicoxa gen. nov., which are characterised by the absence of a setular group on the rostrum, a four-segmented female antennule with elongation of the first segment, the presence of geniculate setae on the distal armature of the antennary endopod, the atrophied condition of the antennary exopod (represented by a single seta or absent), a two-segmented mandibular endopod, prehensile long P1 endopod, setiform elements on the P1 exopod and absence of sexual dimorphism in the male P3 (A. terueae comb. nov.) or development of a stout spine in the male P3 endopod (C. hispida gen. et sp. nov.) (Table
Comparison of morphological characters among nannopodid copepods with modified thoracopods for burrowing ability (female only).
Setal armature | References | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A1 | A2 | Md | Mxl | P1 | P2 | P3 | P4 | P5 | ||||||||
seg | exp seg/setae | exp seg/setae | enp seg/setae | exp seg/setae | exp | enp | exp | enp | exp | enp | exp | enp | exp | enp | ||
Huntemannia | ||||||||||||||||
H. jadensis | 5 | 1/4 | ab | fu/2 | re/2 | 0.0.022 | 020 | 0.023 | 110 | 0.123 | 010 | 0.123 | 010 | 4–5 | 4 |
|
H. micropus | 5 | 1/4 | uk | uk | uk | 0.023 | 020 | 0.021 | 010 | 0.021 | 010 | 0.022 | 010 | 4 | 4 |
|
H. lacustris | uk | uk | uk | uk | uk | 2–3 seg1 | 2* | 0.5* | 1(2)* | 0.5(6)* | 1(2)* | 0.6* | 1* | 5 | 4 |
|
H. biarticulatus | 5 | 1/4 | uk | uk | uk | 0.0.023 | 0.020 | 0.022 | 010 | 0.222 | 010 | 0.222 | 010 | 5 | 4 |
|
H. doheoni | 5 | 1/4 | ab | fu/2 | re/1 | 0.0.022 | 020 | 0202 | 010 | 0112 | 010 | 0112 | 010 | 5 | 4 |
|
Rosacletodes | ||||||||||||||||
R. kuehnemanni | 6 | 1/3 | fu/1 | 1/3 | 1/2 | 0.021 | 020 | 0.022 | 010 | 121 | 010 | 121 | 010 | 5 | 6 |
|
Laophontisochra | ||||||||||||||||
L. maryamae | 4 | re/1 | re/1 | 1/3 | ab | 0.023 | 0.011 | 0.022 | ab | 0.022 | ab | 0.022 | 010 | 4 | 1 |
|
Acuticoxa | ||||||||||||||||
A. biarticulata | 4 | 1/1 | uk | uk | uk | 0.023 | 0.011 | 0.022 | 010 | 0.022 | 010 | 122 | 010 | 4 | 3 |
|
A. ubatubaensis | 4 | ab | re/1 | 1/3 | ab | 0.023 | 0.011 | 023 | 010 | 023 | 010 | 023 | 010 | 4 | 2 |
|
A. terueae comb. nov. | 4 | ab 3 | re/2 | 1/23 | ab | 0.0334 | 0.020 | 0.011(2) | 010 | 0.022 | 010 | 0.0225 | 010 | 4 | 3 |
|
Talpacoxa | ||||||||||||||||
T. brandini | 5 | 1/3 | 1/1 | 1/3 | 1/2 | 023 | 0.020 | 0.020 | 010 | 0.121 | 010 | 0.121 | 010 | 5 | 3 |
|
Concilicoxa gen. nov. | ||||||||||||||||
C. hispida gen. et sp. nov. | 4 | re/1 | ab | 2/1.3 | 1/2 | 0.022 | 0.011 | 022 | ab | 112 | 000 | 112 | 010 | 4 | 1 | the present study |
The authors thank Prof. Rony Huys (the National History Museum, London) for his great help in early identification and useful comments. We also gratefully thank Prof. S. Gómez and two anonymous reviewers for their critical and valuable advice to improve our initial manuscript. This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2018R1A6A3A01012703) to JG Kim and by the Marine Biotechnology Program of the Korea Institute of Marine Science and Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (MOF) (No. 20170431). This work was also conducted with the support offered through the research programme of KIOST (Contract No. PE99813).