Research Article |
Corresponding author: Nadine Dupérré ( nadineduperre@gmail.com ) Academic editor: Cristina Rheims
© 2020 Nadine Dupérré, Charlotte Francisco, Ella Santana-Propper, Ingi Agnarsson, Greta J. Binford.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Dupérré N, Francisco C, Santana-Propper E, Agnarsson I, Binford GJ (2020) Heteroonops (Araneae, Oonopidae) spiders from Hispaniola: the discovery of ten new species. ZooKeys 964: 1-30. https://doi.org/10.3897/zookeys.964.51554
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The Caribbean biodiversity hotspot harbors vast reserves of undiscovered species. A large-scale inventory of Caribbean arachnids (CarBio) is uncovering new species across the arachnid tree of life, and allowing inference of the evolutionary history that has generated this diversity. Herein we describe ten new species of Heteroonops (Oonopidae, or goblin spiders), from Hispaniola: H. scapula sp. nov., H. jurassicus sp. nov., H. aylinalegreae sp. nov., H. verruca sp. nov., H. renebarbai sp. nov., H. yuma sp. nov., H. carlosviquezi sp. nov., H. gabrielsantosi sp. nov., H. solanllycarreroae sp. nov. and H. constanza sp. nov. The occurrence of the pantropical type species Heteroonops spinimanus (Simon, 1891) is reported and new localities are given for: H. validus (Bryant, 1948), H. vega (Platnick & Dupérré, 2009) and H. castelloides (Platnick & Dupérré, 2009). Molecular phylogenies indicate substantial genetic divergence separating these taxa. This work adds to evidence that the depth of diversity in the Caribbean biodiversity hotspot is particularly striking for tiny taxa living in leaf litter.
biodiversity hotspot, Caribbean biogeography, Goblin spiders, molecular phylogeny
The Greater Antilles islands form the most species-rich landmasses in the Caribbean biodiversity hotspot. These islands serve as exceptional systems for studies of species formation and biogeography (
Oonopidae are small (1.0–5.0 mm) yellow, orange to bright red haplogyne spiders. Most members of this family are found living in leaf litter, but some live in canopies (
All 66 specimens examined are from the 2012 CarBio expedition to Dominican Republic, unless otherwise noted. They were all found in leaf litter samples that were sifted in the field and either hand sorted, or extracted through Berlese funnels. Specimens are stored at the Natural History Museum in Vermont, USA (
Specimens were collected and examined in 95% ethanol under a SMZ-U Nikon dissection microscope. A Nikon Coolpix 950 digital camera attached to the microscope was used to photograph all the structures to be illustrated. The digital photos were used to trace proportions and the illustrations were detailed and shaded by referring back to the structure under the microscope. Female genitalia were excised using a sharp entomological needle and submerged in lactic acid to clear internal structures. The structures were photographed and illustrated as explained above. All measurements are in millimeters. For complete morphological description of the genus see
DNA extraction was done with the QIAGEN DNeasy Tissue Kit (Qiagen, Inc., Valencia, CA). We sequenced fragments of the mitochondrial Cytochrome c oxidase subunit 1 (COI) and 16S ribosomal RNA (16S), which are typically effective phylogenetic markers at low taxonomic levels for spiders. We amplified COI with LCO1490-2776 and 16S with 16SF and 16SR using standard protocols (see e.g.,
The taxon sampling in our final dataset included mitochondrial sequences for 37 of 38 Heteroonops from the Dominican Republic in our dataset (Table
Species (ND 17) | sex | type? | Locality | Latitude / Longitude | elev m | CO1 | 16s | GenBank Label | Specimen Name |
---|---|---|---|---|---|---|---|---|---|
H. spinimanus | f | DR Beach trail to Cueva del Puente, Parque Nacional del Este | 18.32902N, 068.80995W | 0 | MT636140 | MT635438 | H._spinimanus_f | H. spin 01-1 | |
H. verruca sp. nov. | m | holotype | DR Cachote Biosphere Reserve | 18.09786N, 071.18925W | 1200 | MT636136 | MT635434 | H._verruca_n_sp_m1 | H. verr 37-1 |
f | paratype | DR Cachote Biosphere Reserve | 18.09786N, 071.18925W | 1200 | MT636137 | MT635435 | H._verruca_n_sp_f1 | H. verr 37-2 | |
m | DR Cachote Biosphere Reserve | 18.09786N, 071.18925W | 1200 | MT636139 | MT635437 | H._verruca_n_sp_m2 | H. verr 37-3 | ||
m | DR Cachote Biosphere Reserve | 18.09786N, 071.18925W | 1200 | MT636138 | MT635436 | H._verruca_n_sp_m3 | H. verr 37-4 | ||
H. validus | m | DR Inside cueva del puente, Parque Nacional del Este | 18.3816N, 068.8017W | 25 | MT636112 | MT635415 | H._validus_m1 | H. val 02-1 | |
f | DR Inside cueva del puente, Parque Nacional del Este | 18.3816N, 068.8017W | 25 | MT636113 | H._validus_f1 | H. val 02-2 | |||
f | DR Inside cueva del puente, Parque Nacional del Este | 18.3816N, 068.8017W | 25 | MT636114 | MT635416 | H._validus_f2 | H. val 02-3 | ||
m | DR Inside cueva del puente, Parque Nacional del Este | 18.3816N, 068.8017W | 25 | MT636115 | H._validus_m2 | H. val 02-4 | |||
m | DR Inside cueva del puente, Parque Nacional del Este | 18.3816N, 068.8017W | 25 | MT636116 | H._validus_m3 | H. val 02-5 | |||
H. carlosviquezi sp. nov. | f | holotype | DR Loma Quita Espuela | 19.34405N, 069.46635W | 200 | MT636111 | MT635414 | H._carlosviquezi_n_sp_f | 7B11-2 |
H. castelloides | m | DR Loma Quita Espuela | 19.34405N, 069.46635W | 200 | MT636124 | MT635423 | H._castelloides_m | H. cast 11-1 | |
H. vega | m | DR Loma Quita Espuela | 19.34405N, 069.46635W | 200 | MT636123 | H._vega_m | H. veg 11-3 | ||
H. yuma sp. nov. | f | holotype | DR Loma Quita Espuela | 19.34405N, 069.46635W | 200 | MT636122 | MT635422 | H._yuma_n_sp_f1 | H. veg 11-1 |
f | paratype | DR Loma Quita Espuela | 19.34405N, 069.46635W | 200 | MT636121 | MT635421 | H._yuma_n_sp_f2 | H. veg 11-2 | |
H. aylinalegreae sp. nov. | m | DR Los Haitises: Cueva la Arena | 19.08013N, 069.4649W | 17 | MT636132 | MT635430 | H._aylinalegreae_n_sp_m3 | H. five 07-1 | |
H. renebarbai sp. nov. | m | holotype | DR Los Haitises: Cueva la Arena | 19.08013N, 069.4649W | 17 | MT636110 | MT635413 | H._renebarbai_n_sp_m | H. six 07-1 |
H. aylinalegreae sp. nov. | m | holotype | DR Parque del Este | 18.355536N, 068.61825W | 46 | MT636128 | MT635427 | H._aylinalegreae_n_sp_m1 | H. five 03-1 |
f | DR Parque del Este | 18.355536N, 068.61825W | 46 | MT645158 | H._aylinalegreae_n_sp_f3 | H. five 03-2 | |||
f | DR Parque del Este | 18.355536N, 068.61825W | 46 | MT636131 | MT635429 | H._aylinalegreae_n_sp_f1 | H. five 03-3 | ||
f | DR Parque del Este | 18.355536N, 068.61825W | 46 | MT636129 | H._aylinalegreae_n_sp_f2 | H. five 03-4 | |||
m | DR Parque del Este | 18.355536N, 068.61825W | 46 | MT636130 | MT635428 | H._aylinalegreae_n_sp_m2 | H. five 03-5 | ||
H. constanza sp. nov. | m | holotype | DR Valle Nuevo (Jurassic Park) | 18.688N, 070.596W | 2100 | MT636125 | MT635424 | H._constanza_n_sp_m | H. cast 24-1 |
f | paratype | DR Valle Nuevo (Jurassic Park) | 18.688N, 070.596W | 2100 | MT636126 | MT635425 | H._constanza_n_sp_f1 | H. cast 24-2 | |
f | paratype | DR Valle Nuevo (Jurassic Park) | 18.688N, 070.596W | 2100 | MT636127 | MT635426 | H._constanza_n_sp_f2 | H. cast 24-3 | |
H. gabrielsantosi sp. nov. | f | paratype | DR Valle Nuevo (Jurassic Park) | 18.688N, 070.596W | 2100 | MT636133 | MT635431 | H._gabrielsantosi_n_sp_f2 | H. one 24-1 |
f | holotype | DR Valle Nuevo (Jurassic Park) | 18.688N, 070.596W | 2100 | MT636135 | MT635433 | H._gabrielsantosi_n_sp_f1 | H. one 24-2 | |
f | paratype | DR Valle Nuevo (Jurassic Park) | 18.688N, 070.596W | 2100 | MT636134 | MT635432 | H._gabrielsantosi_n_sp_f3 | H. one 24-3 | |
H. jurassicus sp. nov. | m | DR Valle Nuevo (Jurassic Park) | 18.688N, 070.596W | 2100 | MT636117 | MT635417 | H._jurassicus_n_sp_m1 | H. jur 24-1 | |
m | DR Valle Nuevo (Jurassic Park) | 18.688N, 070.596W | 2100 | MT636120 | MT635420 | H._jurassicus_n_sp_m2 | H. jur 24-3 | ||
f | DR Valle Nuevo (Jurassic Park) | 18.688N, 070.596W | 2100 | MT636118 | MT635418 | H._ jurassicus_n_sp_f1 | H. jur 24-4 | ||
f | DR Valle Nuevo (Jurassic Park) | 18.688N, 070.596W | 2100 | MT636119 | MT635419 | H._ jurassicus_n_sp_f2 | H. jur 24-5 | ||
H. scapula sp. nov. | f | paratype | DR Valle Nuevo Rd | 18.84633N, 070.74064W | 2983 | MT636109 | MT635412 | H._ scapula_n_sp_f | H. two 22-2 |
m | holotype | DR Valle Nuevo, NP; Valle Nuevo Rd | 18.84633N, 070.74064W | 2983 | MT636108 | H._scapula_n_sp_m | H. two 22-1 | ||
Oonopidae sp 1 | f | DR Los Haitises: Cueva la Arena | 19.08013N, 069.4649W | 17 | MT636142 | MT635440 | Oonopidae_sp_1_DR_f | H. six 07-2 | |
Oonopidae sp 2 | f | PR Mona Island: Bajuga Empalme | MT636141 | MT635439 | Oonopidae_sp_2_Mona_f | H. mona 1 | |||
00392858 Stenoonops portoricensis | f | PR Ranger Station, Guanica Dry Forest | 17.971472N, 066.86795W | 154 | MT636143 | 00392858_S._portoricensis |
For phylogenetic analyses, alignments were done in MAFFT (
Somatic morphology
ALE anterior lateral eye
PLE posterior lateral eye
PME posterior median eye
Genitalia (female)
ar anterior receptaculum
ef epigastric furrow
es epigastric scutum
pr posterior receptaculum
ps postepigastric scutum
wp wing like projections
Genitalia (male)
c bulb
c conductor
e embolus
The ten new species of Heteroonops presented in this work are genetically distinct and distinguishable morphologically. They were all collected in leaf litter samples from forest or cave habitats in Hispaniola ranging from near sea level to 2983 m. Mitochondrial genetic divergences and patterns of relationships belie a deep and old history of Heteroonops on Hispaniola (Fig.
Summary phylogeny of the included species rendering support for the monophyly (multiple samples per species) or exclusivity (single specimens) of each species dealt with here. Species color scheme equals that on map in Figure
H. andros Platnick & Dupérré, 2009, H. aylinalegreae sp. nov., H. carlosviquezi sp. nov., H. castelloides Platnick & Dupérré, 2009, H. castellus (Chickering, 1971), H. colombi Dumitrescu & Georgescu, 1983, H. constanza sp. nov., H. croix Platnick & Dupérré, 2009, H. gabrielsantosi sp. nov., H. iviei Platnick & Dupérré, 2009, H. jurassicus n. sp, H. macaque Platnick & Dupérré, 2009, H. murphyorum Platnick & Dupérré, 2009, H. renebarbai sp. nov., H. saba Platnick & Dupérré, 2009, H. scapula sp. nov., H. singulus (Gertsch & Davis, 1942), H. solanllycarreroae sp. nov., H. spinigata Platnick & Dupérré, 2009, H. spinimanus (Simon, 1891), H. toro Platnick & Dupérré, 2009, H. validus (Bryant, 1948), H. vega Platnick & Dupérré, 2009, H. verruca sp. nov., H. yuma sp. nov.
Mexico, Costa Rica, Bahama Islands, Cuba, Jamaica, Dominican Republic, Puerto Rico, Virgin Islands, Saba, Montserrat and Dominica (H. spinimanus (Simon, 1891) presents a pantropical distribution).
Males are easily diagnosed from all other Oonopidae by the presence of one or two backward-pointing projections on the male palpal endites (Figs
Male holotype from Dominican Republic, La Vega Province, Constanza, Valle Nuevo National Park, 18.84633N, 70.74064W, 2983 m, 26.vi.2012, team CarBio (
The specific epithet is a noun in apposition meaning wings, in reference to the large wing-like structures of the female internal genitalia.
Males are diagnosed from all species by the combination of the following characters: constricted tip of palpal bulb and their bent embolus, wider apically, long conductor reaching the tip of the embolus (Figs
Male (holotype): Total length: 1.9; carapace length: 1.0; carapace width: 0.7. Cephalothorax: Carapace ovoid; shiny, bright orange; pars cephalica flat. Sternum yellow; longer than wide; covered entirely with long dark setae. Endites yellow with one elongated and thin apical backward-pointing projection (Fig.
Female (paratype): Total length: 1.98; carapace length: 0.94; carapace width: 0.74. Cephalothorax: Carapace ovoid; shiny, bright orange; pars cephalica flat. Sternum, labium and chelicerae: as in male. Endites without projection. Eyes: Same as male. Abdomen: Oval; gray; epigastric and postepigastric scuta orange, well sclerotized (Fig.
None.
Dominican Republic, La Vega Province (Fig.
Male holotype from Dominican Republic, La Vega Province, Constanza, Valle Nuevo National Park, ‘Jurassic Park’, 18.688N, 70.596W, 2100 m, 26.vi.2012, team CarBio (
The specific epithet is a noun in apposition taken from the type locality, Jurassic Park, Dominican Republic.
Males are distinguished from all species of the genera by the spatula-shaped tip of the embolus (Fig.
Male (holotype): Total length: 1.93; carapace length: 1.03; carapace width: 0.96. Cephalothorax: Carapace ovoid; shiny, bright orange; pars cephalica slightly elevated. Sternum orange; longer than wide; covered entirely with long dark setae. Endites orange with one very small apical backward-pointing projection (Fig.
Female (paratype): Total length: 2.12; carapace length: 0.92; carapace width: 0.76. Cephalothorax: Carapace ovoid; shiny, yellow; pars cephalica flat. Sternum and labium light yellow. Chelicerae and endites light yellow, not modified. Eyes: as in male. Abdomen: Oval, light beige; epigastric and postepigastric scuta orange, well sclerotized (Fig.
Same data as type specimens: 1♂ (USNMENT 00788060), 1♂ (USNMENT 00788048), 1♀ (USNMENT 00788084); 3♂, 4♀ (
Dominican Republic, La Vega Province (Fig.
Male holotype from Dominican Republic, La Alta Gracia Province, Occidental, San Rafael, del Este National Park, 18.355536N, 68.6182518W, 46 m, 7–8.vi.2012, team CarBio (
The specific epithet is a noun in apposition honoring local arachnologist and CarBio collaborator Aylin Alegre.
Males are diagnosed from all Heteroonops by the combination of the following characters: embolus well sclerotized, not spatulated apically; short conductor not reaching the tip of the embolus (Fig.
Male (holotype): Total length: 1.65; carapace length: 0.79; carapace width: 0.67. Cephalothorax: Carapace ovoid; shiny, light yellow; pars cephalica flat. Sternum light yellow; longer than wide; covered entirely with long dark setae. Endites light yellow with one small apical backward-pointing projection (Fig.
Female (paratype): Total length: 1.89; carapace length: 0.81; carapace width: 0.67. Cephalothorax: Carapace, sternum, labium and chelicerae: as in male. Endites without projection. Eyes: Same as male. Abdomen: Oval; light gray; epigastric and postepigastric light yellow, not well sclerotized (Fig.
1♂ Dominican Republic, Hato Mayor Province, Occidental, San Rafael de Yuma, Parque Nacional los Haitises, Cueva La Arena, 19.08013N 69.4649W, 17♂, 12.vi.2012, team CarBio (
Dominican Republic, La Alta Gracia and Hato Mayor provinces (Fig.
Male holotype from Dominican Republic, Barahona Province, Cachote Biosphere Reserve, 18.09786N, 71.18925W, 1200 m, 7.vii.2012, team CarBio (
The specific epithet is a noun in apposition meaning wart in reference to the male palpal bulb bearing a wart-like projection.
Males can be diagnosed from all species by the wart-like projection on the prolateral side of the bulb (Fig.
Male (holotype): Total length: 1.9; carapace length: 0.95; carapace width: 0.79. Cephalothorax: Carapace ovoid; shiny, bright yellow; pars cephalica flat. Sternum yellow; longer than wide; covered entirely with long dark setae. Endites yellow with one large, median backward-pointing projection (Fig.
Female (paratype): Total length: 2.04; carapace length: 0.98; carapace width: 0.76. Cephalothorax: Carapace, sternum, labium and chelicerae: as in male. Endites without projection. Eyes: Same as male. Abdomen: Oval, light beige; epigastric and postepigastric scuta orange, well sclerotized (Fig.
Same data as type specimens: 2♂ (
Dominican Republic, Barahona Province (Fig.
Male holotype from Dominican Republic, Hato Mayor Province, Occidental, San Rafael de Yuma, los Haitises National Park, outside Cueva La Arena, 19.08013N, 69.4649W, 17m, 12.vi.2012, team CarBio (
The specific epithet is a noun in apposition honoring local arachnologist and CarBio collaborator René Barba.
Males are distinguished from most species by their elongated, thin embolus (Fig.
Male (holotype): Total length: 1.34; carapace length: 0.71; carapace width: 0.59. Cephalothorax: Carapace ovoid; shiny, light yellow; pars cephalica flat. Sternum light yellow; longer than wide; covered entirely with long dark setae. Endites light yellow with an elongated apical backward-pointing projection with rounded tip (Fig.
Female: Unknown.
None.
Dominican Republic, Hato Mayor Province (Fig.
Female holotype from Dominican Republic, Duarte Province, Occidental, San Rafael de Yuma, Loma Quita Espuela, 19.35504N, 70.111W, 200 m, 14.vi.2012, team CarBio (
The specific name is noun in apposition taken from the type locality, San Rafael de Yuma, Dominican Republic.
Females are distinguished from most species by the anterior receptaculum positioned on a narrow, short stalk; from H. vega by their larger anterior receptaculum projecting posteriorly (Fig.
Female (holotype) Total length: 1.86; carapace length: 0.76; carapace width: 0.61. Cephalothorax: Carapace ovoid; shiny, whitish; pars cephalica flat. Sternum whitish; longer than wide; covered entirely with long dark setae. Endites withish, not modified; labium light whitish. Clypeus vertical; short (1/2× radius of ALE). Chelicerae pale yellow; promargin and retromargin without teeth; fangs normal, 1/3 length of chelicerae. Eyes: Six eyes surrounded by black pigmentation; ALE largest, oval; PME squared; PLE smallest, oval; ALE separated by their radius; ALE-PLE touching; PLE-PME touching; PME touching. Abdomen: Oval; yellowish; epigastric and postepigastric scuta pale yellow, not well sclerotized (Fig.
Male: Unknown.
None.
Dominican Republic, Duarte Province (Fig.
Female holotype from Dominican Republic, Duarte Province, Occidental, San Rafael de Yuma, Loma Quita Espuela, 19.35504N, 70.111W, 200 m, 14.vi.2012, team CarBio (
The specific epithet is a noun in apposition honoring Costa Rican arachnologist and CarBio collaborator Carlos Viquez.
Females are easily diagnosed by their umbrella-shaped anterior receptaculum (Fig.
Female: Total length: 2.06; carapace length: 0.96; carapace width: 0.8. Cephalothorax: Carapace ovoid; shiny, light orange; pars cephalica flat. Sternum yellow; longer than wide; covered entirely with long dark setae. Endites yellow, not modified; labium light yellow. Clypeus vertical; short (1/2× radius of ALE). Chelicerae yellow; promargin and retromargin without teeth; fangs normal, 1/3 length of chelicerae. Eyes: Six eyes surrounded by black pigmentation; ALE largest, oval; PME squared; PLE smallest, oval; ALE separated by their radius; ALE-PLE touching; PLE-PME touching; PME touching. Abdomen: Oval; dark grayish-blue with pattern, apically whitish (Fig.
Male: Unknown.
None.
Dominican Republic, Duarte Province (Fig.
Female holotype from Dominican Republic, La Vega Province, Constanza, Valle Nuevo National Park, ‘Jurassic Park’, 18.688N, 70.596W, 2100 m, 26.vi.2012, team CarBio (
The specific epithet is a noun in apposition honoring local arachnologist and CarBio collaborator Gabriel Santos.
Females can be diagnosed from all species by the arch wing-like projections of the internal genitalia and large oval posterior receptaculum (Fig.
Female: Total length: 2.31; carapace length: 0.91; carapace width: 0.84. Cephalothorax: Carapace ovoid; shiny, light yellow; pars cephalica flat. Sternum light yellow; longer than wide; covered entirely with long dark setae. Endites light yellow, not modified; labium light yellow. Clypeus vertical, short (1/2× radius of ALE). Chelicerae light yellow; promargin and retromargin without teeth; fangs normal, 1/3 length of chelicerae. Eyes: Six eyes surrounded by black pigmentation; ALE largest, oval; PME squared; PLE smallest, oval; ALE separated by their radius; ALE-PLE touching; PLE-PME touching; PME touching. Abdomen: Oval; whitish covered dorsally with long dark setae; epigastric and postepigastric scuta light orange, well sclerotized (Fig.
Male: Unknown.
None.
Dominican Republic, La Vega Province (Fig.
Female holotype from Dominican Republic, Duarte Province, Occidental, San Rafael de Yuma, Loma Quita Espuela, 19.35504N, 70.111W, 200 m, 14.vi.2012, team CarBio (
The specific epithet is a noun in apposition honoring local arachnologist and CarBio collaborator Solanlly Carrrero.
Females are diagnosed from all species by their posteriorly protruding epigastric scutum and their oval posterior receptaculum with folded bag-like extension (Fig.
Female (holotype). Total length: 1.37; carapace length: 0.61; carapace width: 0.42. Cephalothorax: Carapace ovoid; shiny, whitish; pars cephalica flat. Sternum whitish; longer than wide; covered entirely with long dark setae. Endites whitish, not modified; labium whitish. Clypeus vertical; short (1/2× radius of ALE). Chelicerae whitish; promargin and retromargin without teeth; fangs normal, 1/3 length of chelicerae. Eyes: Six eyes surrounded by black pigmentation; ALE largest, oval; PME squared; PLE smallest, oval; ALE separated by their radius; ALE-PLE touching; PLE-PME touching; PME touching. Abdomen: Oval; light gray covered dorsally with long dark setae; epigastric scutum protruding, postepigastric scutum thin; scuta light yellow, not well sclerotized (Fig.
Male: Unknown.
None.
Dominican Republic, La Duarte Province (Fig.
Male holotype from Dominican Republic, La Vega Province, Constanza, Valle Nuevo National Park, ‘Jurassic Park’, 18.688N, 70.596W, 2100 m, 26.vi.2012, team CarBio (
The specific name is noun in apposition taken from the type locality, Constanza Province, Dominican Republic.
Both males and females closely resemble H. castelloides Platnick & Dupérré, 2009; males are distinguished by the narrow, elongated palpal bulb and palpal tibia 2× longer than patellae (Fig.
Male (holotype): Total length: 1.79; carapace length: 0.86; carapace width: 0.72. Cephalothorax: Carapace ovoid; shiny, pale yellow; pars cephalica slightly elevated. Sternum pale yellow; longer than wide; covered entirely with long dark setae. Endites pale yellow, with small apical projection (Fig.
Female (paratype): Total length: 2.09; carapace length: 0.85; carapace width: 0.72. Cephalothorax: Carapace ovoid; shiny, yellow; pars cephalica flat. Sternum and labium light yellow. Chelicerae and endites light yellow, not modified. Eyes: as in male. Abdomen: Oval, light beige; epigastric and postepigastric scuta pale yellow, not well sclerotized (Fig.
Dominican Republic, La Alta Gracia Province, Occidental, San Rafael de Yuma, del Este National Park, beach Trail to Cueva del Puente, 18.32902N, 68.80995W, 0 m, 5.vi.2012, team CarBio,1♀ (
Dominican Republic, La Duarte Province, Occidental, San Rafael de Yuma, Loma Quita Espuela, 19.35504N, 70.111W, 200 m, 14.vi.2012, team CarBio, 1♂ (
Dominican Republic, La Alta Gracia Province, Occidental, San Rafael de Yuma, del Este National Park, Cueva del Puente, 18.3816N, 68.8017W, 25 m, 6.vi.2012, team CarBio, 3♂4♀ (
Dominican Republic, La Duarte Province, Occidental, San Rafael de Yuma, Loma Quita Espuela, 19.35504N, 70.111W, 200m, 14.vi.2012, team CarBio, 1♂ (
Observed patterns in our data are consistent with a high probability that our sampling has only detected a small subset of the Heteroonops diversity in Hispaniola. First, we found a total of 66 individuals distributed in 14 Heteroonops species, 10 of which were new, from only eight sampling sites. At a single site in Loma Quita (200 m) we found five species including three that are new (H. yuma, H. carlosviquezi, H. solanllycarreroae) and two that represent new records (H. vega, H. castelloides). Similarly, we found three new species in one locality in a high elevation forest (2100 m) in the Cordillera Central Parque National Valle Nuevo (H. constanza, H. gabrielsantosi, H. jurassicus). Moreover, a fourth new species H. scapula, was discovered in the same park at higher elevation (2983 m). Taxa from both of these localities are phylogenetically widespread reflecting an old most recent common ancestor and high levels of subsequent diversification (Fig.
Despite patterns consistent with high local diversity, there is evidence that some Heteroonops species are wide ranging. Two taxa that represent new records were collected far from their type localities in the Cordillera Central, H. castelloides, and H. validus. Interestingly both of these species have been collected in flight intercept traps (
We thank all the members of the CarBio team, especially those involved in expeditions in the Dominican Republic. We are especially grateful to René Barba Diaz, Aylin Alegre Barosso, Gabriel de los Santos, and Solanlly Carrero Sanchez for help with logistics, organization, and in the field. All material was collected under appropriate collection permits and guidelines: Dominican Republic, Ministerio de Medio Ambiente y Recursos Naturales, no 0577. Funding for this work came from National Science Foundation (DEB-1314749 and DEBs-1050253/1050187) to I. Agnarsson and G. Binford. Additional funds came from the Smithsonian Laboratories of Analytical Biology, a 2013 SI Barcode Network grant to J.A. Coddington and Agnarsson. Thanks to the reviewers Cor Vink and Everton N. L. Rodrigues for positive comments that help improve the manuscript.
Phylogeny in Figure
Data type: Tree topology inferred using Bayesian analyses of mtDNA with each terminal taxon labeled
Explanation note: This tree is the same phylogeny as in Figure