Research Article |
Academic editor: Wanda M. Weiner
© 2020 Claudia M. Ospina-Sánchez, José G. Palacios-Vargas, Grizelle González.
This is an open access article distributed under the terms of the CC0 Public Domain Dedication.
Citation:
Ospina-Sánchez CM, Palacios-Vargas JG, González G (2020) A new Caribbean species of Hylaeanura Arlé, 1966 (Collembola, Neanuridae, Pseudachorutinae). ZooKeys 961: 31-39. https://doi.org/10.3897/zookeys.961.47227
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We here describe a new Collembola species, Hylaeanura emiliae sp. nov., from the Luquillo Experimental Forest in Puerto Rico. We describe H. emiliae sp. nov. as a distinct species based on the enlarged sensilla s3 in antennal segment IV, the absence of modified sensorial setae in abdominal segment IV and the presence of four setae on each dens. An updated key with illustrations for the identification of worldwide species of the genus is included.
Island, Luquillo Experimental Forest, Puerto Rico, subtropical forest, taxonomy
In Puerto Rico, most studies of arthropod community dynamics have been performed in the Luquillo Mountains (
In most studies of litter and soil fauna in the LEF, Collembola are an important group for ecosystem functioning because of their dominant abundance and their key responses to changes in disturbance, altitude and vegetation type (
The genus Hylaeanura was conceived by
The material used to describe the new Hylaeanura species came from the survey of the Collembola microhabitats in the Luquillo Mountains conducted in 2014 and 2015 along three forest types (Tabonuco, Dacryodes excelsa; Palo Colorado, Cyrilla racemiflora and Elfin forest, Tabebuia rigida). Collembola were extracted from soil and litter samples using a Berlese-Tullgren funnel and stored in 95% ethanol. They were cleared using Nesbitt solution and fixed in slides using Mac André II solution (
Abbreviations:
a.s.l. above sea level
Abd abdominal segment
Ant antennal segment
PAO Postantennal Organ
S sensilla
Sgd dorsal guard sensillum of Ant III
Sgv ventral guard sensillum of Ant III
ss sensorial setae
Tita tibiotarsi
Th thoracic segment
Neanuridae
Pseudachorutinae
(modified from
Holotype. Female on slide. Paratypes: 1 female and 1 juvenile on slide. All the type material kept at corresponding author’s institution.
Puerto Rico, Luquillo, Luquillo Mountains, Toro Trail 1, 18°16'40"N, 65°50'53"W; 815 m a.s.l.; ex soil, Cyrilla racemiflora forest type, 18 November 2014. All specimens were extracted using Berlese-Tullgren funnels from samples collected in leaf litter and soil at the Luquillo Mountains. C. M. Ospina and M. Rivera leg.
Female on slide, Puerto Rico, Luquillo, Luquillo Mountains, Toro Trail 2, 18°16'40.3"N, 65°51'01"W, Cyrilla racemiflora forest type, leaf litter, 795 m a.s.l., 18 November 2014, C.M.Ospina leg. One (1) juvenile on slide, Puerto Rico, Luquillo, Luquillo Mountains, Toro Trail 1, 18°16'40"N, 65°50'53"W, Cyrilla racemiflora forest type, leaf litter, 815 m a.s.l., 18 February 2015, C. M. Ospina leg.
Hylaeanura emiliae sp. nov. has an enlarged sensillas S3 and S8 on Ant IV, Abd IV without modified setae, and four setae on each dens.
Length of the holotype 1050 µm (female paratype 653 µm and juvenile paratype 550 µm). Specimens in ethanol without color, body with coarse granulation and without tubercles. Body setae short and smooth, the sensorial setae longer than ordinary setae, both acuminate (Fig.
Head: Antenna smaller (0.47×) than cephalic diagonal. Ant III and IV fused dorsally. Ant IV dorsally with a trilobed apical vesicle; subapical organite absent and dorsoexternal microsensillum, 7 subcylindrical sensilla, S8 hypertrophied, S7 smaller and S3 large; 12 long setae plus i (Fig.
Body: Ordinary body setae smooth, distributed as in Fig.
Legs: Chaetotaxy of legs I–III as follow: Coxae with 3, 5, 7 trochanter with 5, 5, 4; femora 11, 11, 10 and tita with 16, 16, 14 setae (Fig.
Collophore with 3+3 setae; tenaculum with 3+3 teeth and without setae; furcula reduced, manubrium reduce with 6+6 setae in Abd III ventrally; dens with four setae, mucro absent (Fig.
This species is dedicated to the daughter of the senior author, Emilia, who was born while this research was being conducted.
Hylaeanura emiliae sp. nov. is only known from the Luquillo Mountains in the Cyrilla racemiflora forest type, on the Toro Trail between 795–815 m a.s.l.
Hylaeanura emiliae sp. nov. was extracted from leaf litter and soil in both dry and rainy seasons during November 2014 and May and August 2015.
(updated from
1 | Without eyes | 2 |
– | With 2 eyes per side | 3 |
2 | Sensorial seta on Abd IV candle-flame shaped. Dens with a minute mucro present | H. nohbecana Vázquez, Cutz-Pool & Palacios-Vargas, 1998 |
– | Sensorial seta on Abd IV sensillum shaped. Dens without mucro | H. infima (Arlé, 1959) |
3 | Dorsal and ventral guard sensilla sinuous (Sensorial organ Ant Ill). Mandible with two teeth | H. mendoncae Zeppelini & Palacios-Vargas, 2013 |
– | Dorsal and ventral guard sensilla straight, Mandible with 1 or 3 teeth | 4 |
4 | Tenaculum with 2+2 teeth. Mandible with 3 teeth | H. nepalensis (Yosii, 1966) |
– | Tenaculum with 3+3 teeth. Mandible with 1 tooth | H. emiliae sp. nov. |
The description of Hylaeanura emiliae sp. nov. fits with the most recent genus diagnosis proposed by
Hylaeanura emiliae sp. nov. is different from other Hylaeanura due to the enlargement of the sensilla s3 in Ant IV, the absence of modified S setae in Abd IV and the presence of four setae in the dens. Additionally, it has an unpaired seta d0 in the head. This setae is absent in other described species of the genus, but defines a unique difference within head chaetotaxy.
Using the comparative morphology of Hylaeanura presented by
Hylaeanura infima is the smallest species and has a sensillum of Abd IV of setae shape. It differs from the new species by the absence of eyes and the presence of two teeth in the mandible and the presence of three setae in dens (
Hylaeanura nohbecana is the biggest Hylaeanura, and is similar to the new species in the straight shape of the guard sensillum of Ant IV, the differences appearing in the chaetotaxy: the absence of unpaired setae d0 in the head, the presence of setae a3 in Abd II-IV and the position of the ss in p3 in Abd I. Additionally, H. nohbecana has no eyes, the furcula has two small dens each bearing 3 setae and a small vestigial mucro (
The most recently described species, H. mendoncae differs from H. emiliae sp. nov. in the position of ss in Abd I to III in p3. In Abd IV, ss is also in position p3 but in the shape of a candle-flame setae. In Abd V ss is in position p2. The furcula is reduced and dens have 3 setae each without a mucro (
Summary of main characters of described species of Hylaeanura Arlé, 1966.
Species | Total length (µ) | Ventral guard sensillum | Dorsal guard sensillum | Mandible teeth | Eyes per side of head | Shape Abd IV sensillum | Tenaculum teeth | Setae in dens | Setae in manubrium | Mucro |
---|---|---|---|---|---|---|---|---|---|---|
H. infima | 500 | st | st | 2 | 0 | ss | ? | 3 | 2 | – |
H. nohbecana | 1000 | st | st | 3 | 0 | cf | 3+3 | 3 | 2 | + |
H. nepalensis | 700 | st | st | 3 | 2 | ss | 2+2 | 3 | 2 | – |
H. mendoncae | 600 | si | si | 2 | 2 | ss | 2+2 | 3 | 0 (6) | – |
H. emiliae sp. nov. | 850 | st | st | 1 | 2 | ss | 3+3 | 4 | 0 (6) | – |
This research was supported by Grant DEB 1239764 and 1546686 from the US National Science Foundation to the Institute for Tropical Ecosystem Studies, University of Puerto Rico, and to the International Institute of Tropical Forestry (IITF) USDA Forest Service, as part of the Luquillo Long-Term Ecological Research Program. The US Forest Service (Department of Agriculture) Research and Development Unit, and the University of Puerto Rico provided additional support. We thank to María M. Rivera (IITF) for field work help.