Research Article |
Corresponding author: Dongbao Song ( waspsong@foxmail.com ) Corresponding author: Guanghong Liang ( fjlhg@126.com ) Academic editor: Jose Fernandez-Triana
© 2020 Ciding Lu, Jinhan Tang, Wanying Dong, Youjun Zhou, Xinmin Gai, Haoyu Lin, Dongbao Song, Guanghong Liang.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Lu C, Tang J, Dong W, Zhou Y, Gai X, Lin H, Song D, Liang G (2020) A new species of Glyptapanteles Ashmead (Hymenoptera, Braconidae, Microgastrinae) within Macrobrochis gigas (Lepidoptera, Arctiidae, Lithosiidae) in Fujian, China. ZooKeys 913: 127-139. https://doi.org/10.3897/zookeys.913.46646
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The south-east coastal area of Fujian, China, belongs to the Oriental Realm, and is characterized by a high insect species richness. In this work, a new species of Hymenopteran parasitoid, Glyptapanteles gigas Liang & Song, sp. nov. found in Jinjiang within hosts of caterpillars Macrobrochis gigas (Lepidoptera: Arctiidae), is described and illustrated, with differences from similar species. Additionally, we presumed that both parasitoid and host species play very important role in the coevolution and tritrophic interaction between plants, phytophagous insects, and their parasitoids, because these insects probably broke the sporangia and made contributions to their colonization, or some spores were spread for long distances by adult moths after their emergence, or some parasitoids were attracted by the eggs and larvae of these caterpillars, which was also thought to be helpful to spread of spores.
Glyptapanteles, Macrobrochis gigas, new species, Oriental Realm, parasitoid
In an agricultural ecosystem, insect parasitoids play very important roles in the trophic networks and attract much research for their potential in biological control (
During field surveys for wasps parasitizing M. gigas, we discovered a new species of Glyptapanteles Ashmead, 1904 (Braconidae, Microgastrinae) associated with the caterpillars feeding specifically on mosses. This new species was found in the summers of 2015-2016 from Linyuan town (N24°44'40.42", E118°30'55.65") and Yonghe town (N24°42'57.34", E118°35'10.42") in Jinjiang, Fujian, south and east coastal areas of China. Braconidae parasitoids of M. gigas were sampled using two methods: (1) ectoparasitic cocoons of parasitoids coupled with mummies of host larvae were collected from the trunks of trees; (2) and some living larvae at different instar stages were collected every ten days and reared with mosses indoor to obtain the specimens of parasitoid species. Hymenopteran parasitoids were then sorted and identified.
Morphological diagnosis
Parasitoid adult specimens were cleared up and mounted in Canada Balsam or in Euparal® for measurement of appendages under a stereomicroscope (LEICA 205C, Germany). All figures were made using Leica Application Suite (LAS V 4.0) software. One female paratype specimen was sputter gold-coated and examined using a Jeol JSM – 6380 LV Scanning Electron Microscope. For identification of the subfamily Microgastrinae, see van
The types of the newly described species are deposited in the collection of the Parasitoid Wasp Museum of the Institute of Beneficial Insects, College of Plant Protection, Fujian Agriculture and Forestry University (FAFU), Fuzhou, China.
Abbreviations used in this paper are as follows:
POL Postocellar line (minimum distance between posterior ocelli)
OD Posterior ocellus maximum diameter
OOL Ocular-ocellar distance (minimum distance between posterior ocellus and eye).
Glyptapanteles was originally described in 1904 (
Glyptapanteles is a cosmopolitan group of hyper-diverse parasitoid species, which occur in all faunal regions (
Until 2018, more than 122 species were described worldwide (
The female hypopygium is evenly sclerotized from side to side, never with a series of parallel longitudinal creases. Ovipositor sheath short and mostly concealed by hypopygium, its length not more than half of the hind tibia (rarely longer, but if so, hypopygium is large and acutely pointed, concealing most of the ovipositor), sheaths dagger-shaped with only a few setae concentrated near the apex. Petiole on T1 never wider at the apex, the sides either gradually converging distally or parallel and strongly rounded to the apex. The median area on T2 broadening distally and often subtrapezoidal or truncate-trapezoidal, sometimes lateral grooves delimiting the median area are lost among many diverging aciculations and sometimes do not reach the proximal edge of T3; T3 always smooth. Propodeum usually completely or mostly smooth, but often with coarsely sculpture covering all or part of the surface; rarely with a median longitudinal carina, but never with even a trace of the areola. Fore wing with r-m vein absent, so that the small areolet is open distally. Distal half of margin of vannal lobe of hind wing convex or flattened, with or without a fringe of setae. The anterior furrow of metanotum flattened (without sublateral setiferous projections) and glabrous; scutellar phragma exposed or concealed (
Linyuan town (N24°44'40.42", E118°30'55.65") and Yonghe town (N24°42'57.34", E118°35'10.42") in Jinjiang, Fujian, south and east coastal areas of China.
Etymology. The specific epithet is derived from the scientific name of its host M. gigas. Gender is masculine.
Description. Female (holotype). Body length 2.1 mm, fore wing length 2.3 mm.
Head. In anterior view, head approximately orbicular-ovate with antennal sockets slightly above middle level of eyes; face slightly convex, finely punctate associated with long hairs, ratio of FH:FW being 2.0: 2.6 (Fig.
Glyptapanteles gigas Liang & Song, sp. nov. 1 Head approximately orbicular-ovate; face slightly convex with long hairs, FH: FW = 2.0: 2.6 2 Head. 2.3 times as wide as long. Ocelli large, arranged in a low triangle. POL: OD: OOL= 0.7: 0.3: 0.8 3 Antenna longer than body (10.0: 7.9); Flagellomere proportions: 2 L/W = 2.8, 8 L/W = 2.7, 14 L/W = 1.7, L 2/14 = 1.9, W 2/14 = 1.2. F12-15 tightly connected 4 Mesosoma. Note that notauli hardly exist. Propodeum relatively flat and smooth, horizontally rectangle, no median longitudinal carina 5 Wings. Forewing with areolet open; r and 2-SR meeting at a circular arc; 2-SR: r: width of pterostigma = 0.6: 0.8: 0.9. 1-CU1:2-CU1:m-cu = 0.6: 1.0: 0.7. 6 Legs. Hind coxa large, near to T3. Hind tibia approximately 0.95 times as long as hind tarsa; inner hind tibial spurs longer than outer one and about half of hind basitarsus.
Mesosoma (Fig.
Wings (Fig.
Legs (Fig.
Metasoma. T1 smooth, 1.9 times as long as its greatest width, slightly parallel on both sides, gradually in general converging apically and rounded to apex, base broad depression concave, narrowed toward the end (Fig.
7–10 Glyptapanteles gigas Liang & Song, sp. nov. 7 metasoma. T1 smooth, 1.9 times as long as its greatest width, slightly parallel on both sides, base broad depression concave, narrowed toward the end 8 T2 approximately scalariform, the central area inconspicuous with apical width slightly long than central length. T3 1.1 times as wide as long, slightly longer than T2 9 ovipositor short, ovipositor sheath short, about equal to length of the 2th hind tarsus 10 body mostly black. Antennae black brown. 11 Parasitized larva of M. gigas, cocoons and adult of G. gigas. White ellipse indicating adult of parasitoid. Red and white arrows indicating parasitized larva and cocoons of parasitoid respectively. 12 Unparasitized larvae of the host M. gigas.
Color. Adult body mostly black (Fig.
Male. Antenna longer than body (10.0: 7.0), the rest same as female.
This new species is closely related to Glyptapanteles phragmataeciae (You & Zhou, 1990), but it is easily distinguished from it based on T1 slightly parallel on both sides, gradually in general converging apically and rounded to apex (T1 cuneiform); antenna longer than body (antenna shorter than body); vein 1-R1 1.8 times as long as pterostigma (vein 1-R1 1.0 times as long as pterostigma); inner hind tibial spurs longer than outer one and about half of hind basitarsus (inner hind tibial spurs as long as outer one and shorter than half of hind basitarsus).
The parasitoid of genus Glyptapanteles mostly attacks lepidopteran caterpillars, of which very few species attacks insects of Coleoptera (
In this work, G. gigas sp. nov. found in Jinjiang parasitizing caterpillars of M. gigas is described and illustrated, and differences from similar species that may parasitize the moss caterpillars are provided. There are 171 species of Lithosiini (Lepidoptera: Erebidae) recorded in China (
The moth is possibly involved in a mimicry relationship with Eterusia aedea (Linnnaeus, 1763) (Lepidoptera, Zygaenidae) (
On the other hand, we assume this new species and their hosts may play an important ecological role in their trophic interaction between host plants and insects (
This study was financially supported by National Key R & D Program of China (2018YFC1200400 and 2016YFD0200900) and National Natural Science Fund of China (No. 31870641). We also appreciate Dr. Jun Li from Beneficial Insect Institute of Fujian Agriculture and Forestry University who checked the total number of species of genus Glyptapanteles worldwide.