Research Article |
Corresponding author: Zoltán László ( laszlozoltan@gmail.com ) Academic editor: Andreas Köhler
© 2020 Juli Pujade-Villar, Yiping Wang, Wenli Zhang, Noel Mata-Casanova, Irene Lobato-Vila, Avar-Lehel Dénes, Zoltán László.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Pujade-Villar J, Wang Y, Zhang W, Mata-Casanova N, Lobato-Vila I, Dénes A-L, László Z (2020) A new Diplolepis Geoffroy (Hymenoptera, Cynipidae, Diplolepidini) species from China: a rare example of a rose gall-inducer of economic significance. ZooKeys 904: 131-146. https://doi.org/10.3897/zookeys.904.46547
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A new species of the genus Diplolepis Geoffroy, Diplolepis abei Pujade-Villar & Wang sp. nov. is described on host plant Rosa sertata Rolfe × R. rugosa Thunb. from China with an integrative approach based on molecular and morphological data. Diagnosis, distribution and biology of the new species are included and illustrated. This species is the first known rose gall-inducer of economic importance. A review of Eastern Palearctic species of Diplolepis is given and a key to the Chinese fauna is presented.
gall wasp, Kushui rose, new species, phytophagous, taxonomy
The family Cynipidae (Hymenoptera, Cynipoidea) includes around 1400 species, all of them exclusively phytophagous and divided into 12 tribes (
The genus Diplolepis includes six European species (
Diplolepis is morphologically characterized by having both the head and the mesosoma black or reddish brown; antennae 14–15 segmented (except D. hunanensis with only 12) in both sexes and with relatively large, cylindrical flagellomeres; pronotum dorsomedially short; pronotal plate not pronounced; scutellar foveae faint or absent; mesopleuron with a broad, crenulate mesopleural furrow; propodeum rugose and lateral propodeal carinae usually indistinct; metanotal trough broad, apically truncate; forewings moderately or strongly uniformly or partially infuscate, margins with short but distinct cilia; radial cell closed along the wing margin; 2r of forewings usually with a prominent median vein stump anterolaterally projected; nucha dorsally short; hypopygium plough-shaped and hypopygial spine slightly longer than broad, with some sparse short setae (
Diplolepis are known to cause galls mainly on feral roses. Accidental infections on cultivated Rosa rugosa and its hybrids were reported from North America, but neither of the Diplolepis species became serious pests of rose cultivars (
The terminology for the morphology of cynipid gall wasps used in this work follows
Scanning electron microscope (SEM) images of the described species were taken with a FEI Quanta 200 ESEM at high voltage (15 kV) without gold coating in the “Serveis de Microscopia Electrònica” of the University of Barcelona.
Specimens of the new species were collected in Lanzhou City of Gansu Province and they are deposited in the Hymenoptera Collection of Zhejiang Agricultural and Forest University (
Genomic DNA was extracted from two individuals using an ISOLATE II Genomic DNA Kit (Bioline, Germany), following the protocol provided by the manufacturer. The mitochondrial cytochrome c oxidase subunit I (COI) sequences were amplified using the standard LCO1490 and HCO2198 primer pair (
Sequences were downloaded and verified with the BLAST (
Holotype : ♀ deposited in UB with the following labels: ‘Lanzhou (Gansu Province), ex Rosa sertata × R. rugosa, (03.ii.2011) 15.iii.2011, col. Sheng Maoling’ (white label); ‘Diplolepis abei Pujade-Villar & Wang, desig. JP-V-2017’ (red label). Paratypes: 11♀♀ with the same labels as the holotype: 8♀♀ in ZAFU, 3♀♀ in UB.
This species is characterized by having the following morphological characters: head smooth to alutaceous, mesoscutum alutaceous with piliferous punctures, scutellum rugose with a more delicate sculpture in the centre of the disk; legs, including coxae, reddish; forewings hyaline but slightly smoky in both the radial and the 3rd cubital cells, never with a dusky cloud around veins; second metasomal tergite short. It differs from the rest of species known from China because veins of its forewings are not infuscate. In addition, the deciduous galls have numerous long stout sharp-pointed spines unlike other known species. Molecular results: the two sequenced individuals represent one haplotype (GeneBank accession number: MN434062). Based on the BI tree the species is part of a polytomous clade with a group consisting of D. fructuum, D. mayri and D. rosae, and with D. spinosissimae (Fig.
P-distance values for sequences of Diplolepis abei sp. nov. and all available Diplolepis species. Abbreviations: dradi: Diplolepis radicum, drosfol: D. rosaefolii, dfusif: D. fusiformans, dtrif: D. triforma, dspinsa: D. spinosa, degla: D. eglanteriae, dbic: D. bicolor, dbass: D. bassetti, doreg: D. oregonensis, dignot: D. ignota, dvari: D. variabilis, dgrac: D. gracilis, dpoli: D. polita, dnodu: D. nodulosa, dnebu: D. nebulosa, dfruc: D. fructuum, dcalif: D. californica, dmayr: D. mayri, drosa: D. rosae, dnerv: D. nervosa, dspinsi: D. spinosissimae, dspnov: D. abei sp. nov.
dradi | drosfol | dfusif | dtrif | dspinsa | degla | dbic | dbass | doreg | dignot | dvari | dgrac | dpoli | dnodu | dnebu | dfruc | dcalif | dmayr | drosa | dnerv | dspinsi | |
dradi | |||||||||||||||||||||
drosfol | 13.14 | ||||||||||||||||||||
dfusif | 11.81 | 4.55 | |||||||||||||||||||
dtrif | 6.54 | 11.98 | 10.87 | ||||||||||||||||||
dspinsa | 6.60 | 12.59 | 11.87 | 7.15 | |||||||||||||||||
degla | 15.25 | 13.90 | 12.81 | 13.81 | 14.31 | ||||||||||||||||
dbic | 14.17 | 11.54 | 9.57 | 13.56 | 14.73 | 12.06 | |||||||||||||||
dbass | 14.59 | 11.36 | 9.48 | 13.14 | 14.98 | 12.31 | 6.24 | ||||||||||||||
doreg | 6.99 | 12.63 | 10.62 | 7.82 | 8.90 | 13.56 | 11.81 | 12.48 | |||||||||||||
dignot | 11.59 | 10.93 | 9.32 | 10.48 | 11.98 | 11.98 | 10.40 | 11.81 | 10.15 | ||||||||||||
dvari | 11.92 | 10.75 | 9.15 | 10.48 | 11.98 | 11.98 | 10.40 | 11.81 | 10.15 | 0.83 | |||||||||||
dgrac | 13.09 | 9.97 | 8.65 | 11.48 | 12.31 | 12.65 | 10.40 | 10.65 | 10.98 | 5.66 | 5.49 | ||||||||||
dpoli | 14.14 | 11.13 | 8.71 | 12.65 | 13.70 | 12.42 | 5.68 | 2.61 | 11.56 | 10.59 | 10.59 | 9.93 | |||||||||
dnodu | 7.04 | 11.68 | 10.76 | 5.71 | 7.60 | 15.09 | 13.84 | 13.87 | 8.21 | 10.87 | 10.87 | 11.20 | 12.92 | ||||||||
dnebu | 11.76 | 10.75 | 9.15 | 10.32 | 12.15 | 11.81 | 10.23 | 11.65 | 9.98 | 0.17 | 0.67 | 5.49 | 10.43 | 10.70 | |||||||
dfruc | 11.76 | 9.62 | 8.32 | 10.82 | 12.48 | 12.81 | 9.40 | 9.15 | 9.48 | 8.65 | 8.65 | 9.48 | 7.82 | 10.21 | 8.49 | ||||||
dcalif | 5.75 | 12.38 | 11.13 | 6.58 | 6.66 | 14.24 | 14.32 | 14.32 | 7.70 | 11.49 | 11.74 | 11.49 | 13.43 | 6.86 | 11.66 | 10.99 | |||||
dmayr | 11.00 | 8.60 | 7.54 | 10.39 | 12.23 | 12.06 | 9.13 | 8.88 | 9.38 | 8.71 | 8.71 | 9.38 | 7.76 | 10.44 | 8.54 | 2.35 | 10.39 | ||||
drosa | 11.33 | 9.21 | 8.21 | 10.80 | 12.81 | 12.40 | 9.72 | 9.72 | 10.18 | 8.71 | 8.71 | 9.55 | 8.71 | 10.78 | 8.54 | 3.02 | 10.90 | 2.09 | |||
dnerv | 13.46 | 10.27 | 9.76 | 12.39 | 13.69 | 10.23 | 10.83 | 11.44 | 13.78 | 11.09 | 11.09 | 12.48 | 10.80 | 13.06 | 10.92 | 10.23 | 12.40 | 9.71 | 10.14 | ||
dspinsi | 12.27 | 9.36 | 8.67 | 11.48 | 13.52 | 12.50 | 8.84 | 8.50 | 12.25 | 7.91 | 8.08 | 9.61 | 7.71 | 10.85 | 7.91 | 6.03 | 11.91 | 5.61 | 6.29 | 9.62 | |
dspn | 12.89 | 10.13 | 8.89 | 12.00 | 13.03 | 12.26 | 9.15 | 10.10 | 11.05 | 8.81 | 8.63 | 8.80 | 8.49 | 11.54 | 8.63 | 6.38 | 12.19 | 6.90 | 7.94 | 10.11 | 6.39 |
Description. Female. Length. Body length 3.3–3.6 mm (N = 4).
Color
(Fig.
Head
(Fig.
Antenna
(Fig.
Mesosoma
(Fig.
Legs. Tarsal claws simple, without a basal lobe.
Forewing
(Fig.
Metasoma
(Fig.
Male: unknown.
Gall
(Fig.
The new species was collected on the Chinese Kushui rose, a hybrid of Rosa sertata Rolfe × R. rugosa Thunb. which is cultivated mainly in Gansu Province (China) for its oil. Rosa rugosa also occurs at the collection site (a Kushui rose plantation) but no galls were found on them despite growing only a few meters from Kushui roses supporting large numbers of galls. To the best of our knowledge this may be the first known Diplolepis species that causes significant agricultural loss. In Gansu Province (China) the R. sertata × R. rugosa hybrid is commonly planted for its high yields of flowers and oil. The infected shrubs may suffer up to 70% yield loss according to rose oil farmers (
Only females are known (Fig.
In
China (Gansu Province).
Named in honour of the Japanese cynipidologist and friend, Prof. Yoshihisa Abe (Biosystematics Laboratory, Graduate School of Social and Cultural Studies, Kyushu University, Fukuoka, Japan).
According to
Distribution of the 11 species of Diplolepis of the Palearctic; in yellow, the species distributed exclusively in the Eastern Palearctic. Palaearctic map obtained from https://www.google.com/maps/@57.7164944,49.0396796,9792440m/data=!3m1!1e3. The inset image pointing out in red the Gansu Province (and thus the collecting location) was obtained from https://en.wikipedia.org/wiki/Gansu.
Diplolepis rosae is also recorded from India (
The new species, D. abei Pujade-Villar & Wang induces spherical galls with spines resembling D. japonica and, some forms of D. nervosa in the Palaearctic Region. However, D. abei differs from these species by producing galls with relatively longer, pointed, hard and woody spines. Adults of D. nervosa differ from the new species by having POL slightly longer than OOL, the scutum coriaceous, the scutellum strongly elongated (nearly 2.0 times longer than wide) with subparallel margins and slightly constricted basally, the scutellar foveae present (large, transversely ovate and smooth) and forewings are hyaline (neither with infuscate areas nor smoky marks); on the other hand, in D. japonica the radial cell is shorter (around 2.0 times as long as wide), forewings are hardly infuscate around radial cell, the face is coarsely rugose, the mesoscutum is smooth and the 2nd tergite occupies more than half the length of metasoma.
a forewing of D. flaviabdomenis b forewing of D. hunanensis c forewing of D. minoriabdomenis, and d forewing of D. nr japonica e head in frontal view of D. nr japonica (reused from
1 | Radial cell relatively long, longer than 2.5 times as long as broad (Figs |
2 |
– | Radial cell shorter, around 2.0 times as long as broad (Fig. |
3 |
2 | Radial cell closed, with infuscate veins and 2r vein without projection into the radial cell (Fig. |
D. flaviabdomenis |
– | Radial cell partially open in margin, without infuscate veins and 2r vein with a projection into the radial cell (Fig. |
D. abei sp. nov. |
3 | Head strongly transverse in frontal view, 1.7 times wider than high (Fig. |
4 |
– | Head trapezoid-shaped in frontal view, around 1.5 times wider than high (Fig. |
5 |
4 | Vertex and mesoscutum smooth and shiny. Occiput coarsely punctured. From Japan and Korea | D. japonica |
– | Vertex (Fig. |
D. japonica |
5 | Antennae 12-segmented, with scapus and pedicel yellowish-brown. POL around 2.0 times longer than OOL. Parapsidal lines absent, almost invisible. Radial cell closed (Fig. |
D. hunanensis |
– | Antennae 14-segmented, with scapus and pedicel black. POL around 3.0 times longer than OOL. Parapsidal lines distinct and extending almost the entirely length of mesoscutum. Radial cell completely open in margin (Fig. |
D. minoriabdomenis |
Diplolepis abei is the first Diplolepis associated with a gall from China; D. flaviabdomenis, D. hunanensis and D. minoriabdomenis were described from material collected by Malaise traps (
Finally, D. abei Pujade-Villar & Wang is morphologically closely related to ‘rosae’ clade according to
We are grateful to Yoshihisa Abe (Kyushu University, Fukuoka, Japan) for his comments regarding Diplolepis japonica and the gall of the species described here. The authors are grateful to Chris Looney (Washington State Department of Agriculture, Olympia, United States) for his review, comments and suggestions of the manuscript. The authors are also thankful for Jessica Awad, Yuanmeng M. Zhang and Evandson J. dos Anjos-Silva for their reviews on the submitted manuscript. The molecular work was done at the Interdisciplinary Research Institute on Bio-Nano-Sciences of Babeș-Bolyai University, Treboniu Laurian 42, 400271, Cluj-Napoca Romania. The study was supported by the National Natural Science Foundation of China (31472032 and 31071970) and Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholars (LR14C040002).