Research Article |
Corresponding author: Xiao-Yan Liu ( yanziliu52@163.com ) Corresponding author: Ding Yang ( dyangcau@126.com ) Academic editor: Christopher Borkent
© 2020 Qi-Cheng Yang, Qi-Fei Liu, Zhao-Hui Pan, Xiao-Yan Liu, Ding Yang.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Yang Q-C, Liu Q-F, Pan Z-H, Liu X-Y, Yang D (2020) Nephrotoma Meigen (Diptera, Tipulidae) from Xizang Autonomous Region, China. ZooKeys 973: 123-151. https://doi.org/10.3897/zookeys.973.46384
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Eight species of the genus Nephrotoma were previously known to occur in Xizang Autonomous Region. Here, three species are added to the fauna of Xizang. Among them two species, N. beibengensis sp. nov. and N. hanae sp. nov. are described and illustrated as new to science, and one species, N. evittata Alexander, 1935 is recorded from Xizang for the first time. The following four species are redescribed: N. claviformis Yang & Yang, 1987, N. didyma Yang & Yang, 1987, N. nigrohalterata Edwards, 1928, and N. xizangensis Yang & Yang, 1987. A key to the species of Nephrotoma from Xizang is presented.
Crane flies, new species, taxonomy, Tibet, Tipulinae
The genus Nephrotoma Meigen is one of the largest genera in the family Tipulidae. It is distributed worldwide with 163 known taxa from the Palaearctic Region and 127 taxa from the Oriental Region. Until now, 96 taxa are known in China (
Xizang Autonomous Region (hereafter referred to as Xizang) is located in southwestern China and on the Qinghai-Tibet Plateau. This area is in the Palaearctic Region and on the border of the Oriental Region. With the formation and uplift of the Qinghai-Tibet Plateau, it has an average altitude of more than 4,500 m and a wide elevation range (
The specimens were studied and illustrated with a ZEISS Stemi 2000-c stereo microscope. Details of coloration were checked in specimens immersed in 75% ethyl alcohol (C2H5OH), except the dried specimens of N. claviformis Yang & Yang, 1987. Genitalic preparations of males were made using lactic acid solution (C3H6O3 > 85%) heated in a water-bath to 95 °C for 4–6 minutes. After examination, it was transferred to fresh glycerin (C3H8O3) and stored in a microvial pinned below the specimen. Type specimens are deposited in the Entomological Museum of China Agricultural University (
The morphological terminology mostly follows
1 | Vertex with triangular spots near inner margin of eye; prescutum with narrow black margin ( |
N. distans Edwards, 1928 |
– | Vertex and prescutum not as above | 2 |
2 | Mediotergite with a pair of black spots; tergite 9 with black caudal margin, lateral angle produced into a decurved spine ( |
N. libra Alexander, 1951 |
– | Mediotergite and tergite 9 not as above | 3 |
3 | Scutellum mainly black or dark brown | 4 |
– | Scutellum mainly yellow | 5 |
4 | Occipital marking square, extended anteriorly to inner margin of eye (Figs |
N. beibengensis sp. nov. |
– | Occipital marking not as above; thoracic pleuron with obvious massive black spots; tergite 9 without deep median notch | 6 |
5 | Mediotergite and scutellum with brownish longitudinal spot at middle | 7 |
– | Mediotergite and scutellum not as above | 8 |
6 | Lateral prescutal stripe straight; tergite 9 with a pair of rod-like projections on posterior margin (Figs |
N. claviformis Yang & Yang, 1987 |
– | Anterior end of lateral prescutal stripe bent outward (Figs |
N. nigrohalterata Edwards, 1928 |
7 | Posterior margin of mediotergite slightly dark; tergite 9 with a pair of large aduncous protuberances on posterior margin ( |
N. inorata Alexander, 1951 |
– | Posterior margin of mediotergite not as above; tergite 9 with a pair of short sharp protuberances on posterior margin (Figs |
N. evittata Alexander, 1935 |
8 | Occipital marking subtriangular; scutellum with pale brown middle stripe and brown posterior margin (Fig. |
N. xizangensis Yang & Yang, 1987 |
– | Occipital marking and scutellum not as above | 9 |
9 | Occipital marking dark brown, bell-shaped, extended to top of vertex; tergite 9 strongly protruded at posterior margin (Figs |
N. didyma Yang & Yang, 1987 |
– | Occipital marking and tergite 9 not as above | 10 |
10 | Posterior margin of tergite 9 produced at middle, with a pair of pointed lateral projections; sternite 8 depressed at posterior margin, medially with a sickle fleshy appendage bearing long apical hairs (Figs |
N. hanae sp. nov. |
– | Posterior margin of tergite 9depressed at middle, without pointed lateral projections; sternite 8 with a very deep and narrow notch at posterior margin ( |
N. kaulbacki Alexander, 1951 |
Frontal tubercle raised into discoid protuberance. Occipital marking dark brown, square, anteriorly extending to eye. Mediotergite with an I-shaped, dark-brown, longitudinal stripe. Legs approximately three times as long as body (Figs
Holotype
male (
Male (n = 2): body length 10.4–10.5 mm, wing length 10.0 mm, antenna length 2.5 mm.
Head
(Figs
Thorax
(Figs
Abdomen
(Fig.
Hypopygium
(Figs
Nephrotoma beibengensis sp. nov. 4 Hypopygium, lateral view 5 inner gonostylus, lateral external view 6 outer gonostylus, lateral external view, before softened 7 tergite 9, dorsal view, softened 8 tergite 9, dorsal view. before softened 9 hypopygium, ventral view. Abbreviations: bk = beak, d ct = dorsal crest, gp = gonapophyses, l bk = lower beak, i gonst = inner gonostylus, o b lb = outer basal lobe, o gonst = outer gonostylus, pct= posterior crest, tg = tergite, st = sternite. Scale bars: 0.5 mm (4, 7–9); 0.1 mm (5, 6).
China (Xizang).
This new species is similar to N. globate Alexander, 1951 from India, but the latter differs in the following characters: tergite 9 with lateral projections tipped with two or three spicules; outer gonostylus long and slender; inner gonostylus long and narrow, lower beak like a slender blackened rod; sternite 8 with two tufts of hair.
This species is named after the type locality, Beibeng, Xizang, China.
Nephrotoma claviformis Yang & Yang, 1987: 129. Type locality: China: Xizang, Nyingchi Co.
Spots on head velvety. Vertex with rectangular, dark-brown spots near inner margin of eye. Scutellum dark brown. Mediotergite with a brown longitudinal spot at middle (Fig.
2 males (
Males (n = 2): body length 11.0–11.1 mm, wing length 11.8–12.0 mm, antenna length 3.0 mm.
Head
(Figs
Thorax
(Figs
Abdomen
(Fig.
Hypopygium
(Figs
China (Xizang).
This species is similar to N. distans Edwards, 1928 from Xizang, the inner gonostylus is similar, but the latter differs in the following characters: occipital marking triangular, scutellum and postnotum pale; tergite 9 without rod-like projections on posterior margin.
Nephrotoma didyma Yang & Yang, 1987: 131. Type localty: China: Xizang, Mainling Co.
Pronotum yellow with a brownish spot on lateral side. Mediotergite yellow with dark-brown posterior margin (Figs
4 males (
Male (n = 13): body length 9.5–12.0 mm, wing length 10.0–12.0 mm, antenna length 3.0–4.0 mm.
Head
(Figs
Thorax
(Figs
Abdomen
(Fig.
Hypopygium
(Figs
Nephrotoma didyma Yang & Yang, 1987. 20 Hypopygium, lateral view 21 outer gonostylus, lateral external view 22 tergite 9, dorsal view, softened 23 hypopygium, ventral view 24 inner gonostylus, lateral external view, softened 25 inner gonostylus, lateral external view, before softened. Scale bars: 0.5 mm (20, 22, 23); 0.1 mm (21, 24, 25).
China (Xizang).
Hypopygium of this species is very specific; no similar species.
Nephrotoma distans Edwards, 1928: 700. Type locality: China: Tibet, Rongshar Valley.
Vertex with triangular black spot near inner margin of eye. Prescutum with narrow black margin. Scutellum dark. Wing completely brownish. Gonapophyses of adminiculum hooked (
China (Sichuan and Yunnan).
Nephrotoma evittata Alexander, 1935: 200. Type locality: China: Szechwan, Shin-Kai-Si, Mount Omei.
Antenna mainly dark brown except scape yellow, pedicel and first flagellomere brown; both ends of flagellomere with obvious expansion (Fig.
2 males (
Male (n = 6): body length 11.0–12.5 mm, wing length 11.0–12.0 mm, antenna length 2.5–3.5 mm.
Head
(Figs
Thorax
(Figs
Abdomen
(Fig.
Hypopygium
(Figs
China (Sichuan, Yunnan and Xizang).
First record for Xizang. This species is similar to N. impigra impigra Alexander, 1935 from China (Hubei, Sichuan, Zhejiang, Fujian, Guizhou, Jiangxi), but the latter differs in the following characters: occipital marking distinct; scutellum dark brown. The hypopygium of this species is consistent with holotype, and we found that its occipital marking is variable. We think the difference between the veins is caused by the origin, which is also variable.
Occipital marking variable, small, brown. Antennal scape yellow, pedicel brown, flagellum brownish black (Figs
Holotype
male (
Male (n = 4): body length 10.3–10.6 mm, wing length 11.0–11.6 mm, antenna length 2.8–3.0 mm.
Head
(Figs
Thorax
(Figs
Abdomen
(Fig.
Hypopygium
(Figs
Nephrotoma hanae sp. nov. 37 Hypopygium, lateral view 38 outer gonostylus, lateral external view 39 hypopygium, ventral view 40, 41 inner gonostylus, lateral external view 42 tergite 9, dorsal view. Abbreviations: bk = beak, d ct = dorsal crest, l bk = lower beak, i gonst = inner gonostylus, o b lb = outer basal lobe, o gonst = outer gonostylus, tg = tergite, st = sternite. Scale bars: 0.5 mm (37, 39, 42); 0.1 mm (38, 40, 41).
China (Xizang).
This new species is similar to N. korpa Alexander, 1967 from India (Sikkim), but the latter differs in the following characters: posterior vertex with three brown spots at narrowest point; projections of tergite 9 smaller, but lateral projections without spines; outer gonostylus unusually long, basally expanded.
This species is named after the collector, Shaolin Han.
Nephrotoma inorata Alexander, 1951: 1096. Type locality: China: south-eastern Tibet, Eong To Valley.
Frontal tubercle slightly forked, clover-shaped. Scutum and scutellum with brownish longitudinal spot at middle. Posterior margin of mediotergite slightly darker. Sternite 8 with long hairs, but without process at posterior margin (
China (Xizang), India (W Bengal).
Nephrotoma kaulbacki Alexander, 1951: 1094. Type locality: China: eastern Tibet, Poshö, Kyari Dzong.
Antennal scape and pedicel pale yellow, flagellum black. Scutellum and mediotergite without spot. Tergite 9 with a deep median notch. Sternite 8 with a very deep and narrow notch at posterior margin. Gonapophyses of adminiculum hooked (
China (Xizang).
Nephrotoma libra Alexander, 1951: 1092. Type locality: China: Tibet, Gyantse.
Antennal scape reddish brown, pedicel blackish brown, flagellum black. Mediotergite with a pair of black spots. Tergite 9 with thick, black caudal margin, lateral angle produced into a decurved spine. Sternite 8 without process. Sternite 9 with a finger-like process. Gonapophyses of adminiculum hooked (
China (Xizang).
Nephrotoma nigrohalterata Edwards, 1928: 700. Type locality: China: Szechwan-Tibet border, Yien-Long-Shien.
Nephrotoma attenuata Alexander, 1935: 135. Type locality: China: Szechwan-Tibet border, Yien-Long-Shien.
Vertex with round, dark-brown spot near inner margin of eye; dorsal part of rostrum including nasus brownish black. Antenna black (Figs
12 male (
Male (n = 12): body length 10.5–12.5 mm, wing length 10.5–12.5 mm, antenna length 3.5–5.0 mm.
Head
(Figs
Thorax
(Figs
Abdomen
(Fig.
Hypopygium
(Figs
Nephrotoma nigrohalterata Edwards, 1928. 46 Tergite 9, dorsal view, softened 47 hypopygium, ventral view 48 outer gonostylus, lateral external view, before softened 49 inner gonostylus, lateral external view 50 hypopygium, lateral view 51 outer gonostylus, lateral external view, softened. Scale bars: 0.5 mm (46, 47, 50); 0.1 mm (48, 49, 51).
China (Sichuan, Xizang)
This species is similar to N. geniculata Yang & Yang, 1987 from China (Hubei, Inner Mongolia, Ningxia, Sichuan), but the latter differs in the following characters: without occipital marking; dorsal part of rostrum including nasus yellow. Posterior margin of sternite 8 undepressed, without fleshy lobe ventrally.
Nephrotoma xizangensis Yang & Yang, 1987: 129. Type locality: China: Xizang, Nyingchi Co.
Antennal scape yellow, pedicel pale brown, flagellum mainly dark brown, except first flagellomere pale brown (Fig.
2 males (
Male (n = 3): body length 11.5–12.5 mm, wing length 11.5–12.5 mm, antenna length 3.5–4.0 mm.
Head
(Figs
Thorax
(Figs
Abdomen
(Fig.
Hypopygium
(Figs
China (Xizang).
This species is similar to N. flavonota Alexander, 1914 from Japan (Honshu, Shikoku, Kyushu), China (Zhejiang, Fujian, Hainan), but the latter differs in the following characters: Sternite 8 sheathing very slightly narrowed outwardly, terminating in two broad lobes separated by a V-shaped emargination filled with pale membrane; lobes bearing abundant long yellow setae.
Dr Daicho Kato and one anonymous reviewer are thanked for providing useful comments on an earlier draft of this paper. Thanks to the South-East Tibetan plateau Station for observations and research of alpine environments and for helping in the collection; Ding Huiyi (principal of Damu Township Primary School in Motuo County) helped us with the collection of specimens. We thank Mr Song Zhenqi for providing ecological photos and our friend Guo Yanqing for his help. The data in this research comes from the database of National Digital – Museum of Animal Specimens. This project was supported by the Key Project of Science-technology Basic Condition Platform from the Ministry of Science and Technology of China (no. 2005DKA21402), the Collaborative Innovation Center of Research and Development on Tibetan Characteristic Agricultural and Animal Husbandry Resources (the Plateau Ecology Research Platform), and the Special Project Fund for the Development of Local Colleges and Universities supported by the Central Finance in 2016 (the Investigation of Insect Resources in Southeast Xizang).