Citation: Pedroso DR, Baptista RLC (2014) A new troglomorphic species of Harmonicon (Araneae, Mygalomorphae, Dipluridae) from Pará, Brazil, with notes on the genus. ZooKeys 389: 77–88. doi: 10.3897/zookeys.389.6693
A new species of Harmonicon F. O. Pickard-Cambridge, 1896 (Araneae, Dipluridae) is described, from a medium-sized lateritic cave in Parauapebas, Pará, Brazil. The male holotype and only specimen known of H. cerberus sp. n. was found near the entrance of Pequiá cave. This taxon is the fourth species described and the southernmost record for the genus. The new species displays some troglomorphic characteristics, such as reduction and merging of the posterior median and both pairs of lateral eyes and pale yellow to light brown coloration. Both characters are diagnostic when compared to the normal separated eyes and reddish to dark brown of other Harmonicon species. Other diagnostic characteristics are isolated, long, rigid setae distal to the lyra and the shape of the copulatory bulb. This is the second troglomorphic mygalomorph species from Brazil and the first from the Amazonian region.
Amazonia, biodiversity, cave, Diplurinae, Neotropics, taxonomy
Harmonicon F. O. Pickard-Cambridge, 1896 is a Neotropical genus of Mygalomorphae, belonging to the family Dipluridae. There are three described species from the Amazon region (
The new species was found in the entrance of Pequiá cave, a medium-sized lateritic cave in the Floresta Nacional de Carajás, Parauapebas, Pará, Brazil. One of approximately 1, 100 caves in iron ore deposits found in Carajás, Pequiá cave is situated at 06°05'15" S, 50°07'13"W (DMS), circa 427 m above sea level (
There are several studies on the cave fauna of the iron ore cave region of Carajás (ex.
This is the second troglomorphic mygalomorph species from Brazil, but the first species from the Amazonian region. Recently, a troglobitic Theraphosidae was described from Bahia state (
The color pattern was based on a specimen preserved in 75% ethanol. Observations, photographs and measurements were made with an Olympus stereoscopic microscope. Measurements are given in millimeters, unless otherwise noted. Cephalothorax length was measured from the posterior border to the anterior margin of the clypeus. Total length was measured from the posterior border of the anal tubercle to the anterior margin of the clypeus, not including the spinnerets. Each article of the pedipalp and legs was measured in retrolateral view, from the basal condylus to the distal one. Photographs were taken with a Sony Cybershot DSC-V1 camera attached to the stereomicroscope. The software package COMBINEZ, version COMBINEZP (
The following abbreviations are used: ALE = anterior lateral eyes; AME = anterior median eyes; ITC = inferior (or unpaired) tarsal claws; PLE = posterior lateral eyes; PLS = posterior lateral spinnerets; PME = posterior median eyes; PMS = posterior median spinnerets; STC = superior (or paired) tarsal claws. Spines (or macroseta): ap = apical; p = prolateral; pld = prolaterodorsal; plv = prolateroventral; r = retrolateral; rld = retrolaterodorsal; rlv = retrolateroventral; v = ventral; MNRJ = Museu Nacional, Universidade do Brasil/Universidade Federal do Rio de Janeiro, Brazil.
Harmonicon F. O. P.-Cambridge, 1896: 755;
http://zoobank.org/9AE08814-D3FA-4DCC-9F8E-1493D6CE7E13
http://species-id.net/wiki/Harmonicon_cerberus
Figs 1–11, Map 1 , Table 1This species may be easily recognized by the fusion of the PME and all lateral eyes (Figs 1–3), elongated chelicerae, and pale coloration (Figs 1–2). Another diagnostic trait is the strongly thickened setae near the lyra (Fig. 6). In other Harmonicon, similar setae are found, but they are never so thickened. In prolateral view, the globose bulb, with a strong constriction around the basis of the embolus, resembles Harmonicon oiapoqueae, in contrast to the piriform bulb, regularly tapering toward the embolus, in Harmonicon audeae. The embolus is slightly longer than the bulb itself (ratio 1.3), similar to Harmonicon audeae (1.2), but shorter than in Harmonicon oiapoqueae (1.6).
Male holotype from Brazil: Pará: Parauapebas, Floresta Nacional de Carajás, Pequiá Cave, near entrance, September 2003, Bittencourt, R. (MNRJ 04319).
The specific epithet “cerberus” is an apposition noun and a reference to the three-headed watchdog that guards the entrance to the underworld, the Hades, in Greek mythology.
Male (holotype, Figs 1–11). Measurements: carapace 10.7 long, 9.3 wide, chelicerae 4.4. Abdomen 14.5 long, 6.6 wide. Spinnerets: PMS 2.1 long, 2.0 apart; PLS, total length 18.1, basal article 4.9, middle 6.2, distal 7.0, 2.0. apart; respectively. Legs: see Table 1.
Harmonicon cerberus sp. n. Male holotype: 1 habitus 2 carapace, dorsal view 3 eyes, dorsal view 4 abdomen, dorsal view 5 sternum, ventral view.
Harmonicon cerberus sp. n. Male holotype: 6 maxillae and lyra, ventral view 7 palp and bulb, retrolateral view; copulatory bulb 8 prolateral view 9 frontal view; tibia and metatarsus of leg I 10 retrolateral view 11 ventral view.
Harmonicon cerberus sp. n. male holotype. Length of leg articles.
Leg I | Leg II | Leg III | Leg IV | |
---|---|---|---|---|
Fe | 13.2 | 12.1 | 11.2 | 13.6 |
Pa | 5.7 | 4.9 | 4.7 | 5.0 |
Ti | 10.2 | 9.9 | 9.4 | 11.9 |
Mt | 13.8 | 11.9 | 13.1 | 17.2 |
Ta | 9.3 | 8.8 | 8.0 | 10.0 |
Total | 52.2 | 47.6 | 46.4 | 57.7 |
Carapace (Figs 1, 2) length/width 1.15; flat, cephalic area slightly raised, thoracic furrows shallow and wide. Fovea: 1.0 wide; deep, straight. Carapace with many short, thin setae, interspersed with some longer and thicker setae; border with abundant long and thick setae pointing out, increasing in number towards posterior angles. Clypeus 0.3, frontal margin bearing 9 thick, long, erect setae (Fig. 3). Eye tubercle (Fig. 3) length 0.9, width 1.9, with sparse thin setae separating eyes of both sides and thicker, longer setae at the anterior and posterior borders. AME elliptical, with a milky lens, yellowish brown background, no retina or eye pigments visible. Left AME larger than right one. All other eyes (ALE, PME and PLE) fused in an asymmetrical, crescent shaped, lateral eye mass, with irregular borders, covered by a thin lens, with uniform white background. Right lateral eye mass thinner, shorter and with a more pronounced notch than the left one. Eye row curvature not definable, but AME anterior border a little advanced in relation to anterior border of lateral eye mass (Fig. 3). Right AME 0.3, AME–AME 0.3, right lateral eye mass 0.6 long. Chelicerae (Fig. 2) length/carapace length 0.41, 11 and 12 teeth on promargin, on the left and right chelicera, respectively. Maxillae (Figs 5, 6) length\width: 2.1. Cuspules: 36 spread over ventral inner heel. Lyra at the ventral side of the maxilla, asymmetrical, formed by 4–5 modified thick, long setae, increasing in size from basal to distal one, strongly curved at apical portion, apex just tapering to a point. Right lyra with just 4 setae, left lyra with 5 large setae and a very small, thinner basal one. Thick, erect, regularly curved setae (Fig. 6, arrow) placed distally and a bit internally in relation to lyra, in number of 4 at the right maxilla and 3 at the left one. Labium: length/width 0.8, no cuspules. Labio-sternal groove deep, with elongated sigilla. Sternum (Fig. 5) length (up to labium border) 5.3, width 4.7. Posterior angle in a blunt point, not separating coxae IV. Sigilla: three pairs, elliptical, increasing in size backwards, all far from margin by its own size. Palp long, without spines at retrolateral side, one prolateral spine at distal third of femur, 2 prolateral spines at tibia, distal one longer and thicker. Tibia: length 6.3, thin and long, with similar diameter throughout, length/width 9.1. Leg formula 4123. Legs covered with more abundant short, thin, horizontal black setae and with many longer, thicker, erect black setae. Leg I with modified tibia and metatarsus, forming a retrolateral clasping mechanism (Figs 10–11). Tibia I with a retrolateral distal spur (or apophysis) relatively long, somewhat curved, blunt, bearing a curved, pointed spine at tip. Metatarsus I with small retrolateral tubercle, situated distally to basis of first ventral spine. Tarsal trichobothria much longer than covering setae, placed in a row along the midline of dorsal face. Scopula undivided, covering distal half of metatarsus I and distal third of metatarsi II-III; all tarsi covered with scopula throughout length. All tarsi flexible, with abundant cracks. Spines: leg I: femur d2-2-0 left, d2-1-0 right, pld0-2-1 left, pld1-1-1 right, rld1-2-1 left, rld1-1-2 right; patella 0; tibia p1-0-1, r1-0-1 left, r1-0-0 right, v1-1-1ap (apophysis) left, v2-1-1ap (apophysis) right; metatarsus p0-1-0, v1-2-0 left, v1-2-1ap right; leg II: femur d2-2-0 left, d3-1-0 right, pld1-3-1 left, pld1-2-1 right; patella 0; tibia p1-1-1 left, p1-0-1 right, v2-1-1ap left, v1-1-1ap right; metatarsus pld0-1-0, v1-2-2ap; leg III: femur d2-1-0 left, d3-1-0 right, pld0-2-1, rld1-1-1 left, rld1-2-1 right; patella 0, tibia plv0-1-0 right, pld1-0-1 right, rld1-2-1, v2-2-2ap; metatarsus d2-1-1 left, d1-1-1 right, pld2-2-1 left, pld1-2-0 right, rld1-3-0 left, rld1-3-1 right, v2-1-3ap left, v1-1-2ap right; leg IV: femur d2-1-0, pld1-2-1 left, pld0-2-1 right, rld2-3-1 left, rld2-1-2 right; patella 0, tibia pld1-1-0 left, pld1-2-0 right, rld1-2-2(1ap) left, rld1-2-1 right, v2-2-2ap; metatarsus d1-1-1 left, d2-1-0 right, pld2-1-1 left, pld2-2-1 right, rld2-3-2 left, rld1-2-1 right, v2-1-2ap. Claws: ITC without teeth. Teeth at STC: leg I inner row 4–5, outer row 10–13; leg II inner row 4–5, outer row 10–12; leg III inner row 3, outer row 9–11; leg IV inner row 2–3, outer row 7–10. Bulb (Figs 7–9) globose, with moderately long embolus, a little longer than basis (ratio 1, 3). Bulb with prolateral face convex, gently and uniformly curved, retrolateral face convex at basis and concave at end portion, due to an abrupt curve, forming a strong constriction of bulb near basis of embolus. Embolus with a broad basis, regularly tapering to tip, both in prolateral and retrolateral views. In frontal view, embolus placed at prolateral margin of bulb, slightly curved initially, straight through most of its length, with apex bent retrolaterally (Fig. 9). Also, there is a strong bulge of bulb near embolus basis at same view.
Color pattern (in 75% ethanol). Carapace pale yellow, with thoracic furrows and cephalic area just a little darker, with orange hue, chelicerae light reddish brown, labium, sternum and leg coxae grayish yellow, sigillae darker, with orange hue, other leg articles brownish yellow. Abdomen grayish yellow, with abundant long, thick black hairs.
Troglomorphism. The pale body, fused and reduced eyes and the elongated chelicerae (Figs 1–3) observed in Harmonicon cerberus sp. n. seem to be troglomorphic. Harmonicon species display usually a highly contrasting color pattern, with a reddish carapace, a black, dark brown or reddish brown abdomen and dark brown legs. Eyes of Harmonicon tipically are without modification, with two large AME, surrounded by distinct ALE, PME and PLE. The retina and pigmentation of the AME are easily seen through the clear crystalline lens. In contrast, the AME of the new species bears a milky crystalline and no trace of a retina or pigmentation are visible behind it. The ALE and AME of the other Harmonicon bear distinct crystalline and spherical lenses. However, ALE, PME and PLE of Harmonicon cerberus sp. n. are fused, forming an irregular and asymmetrical white macula, covered by a shallow and irregular lens (Fig. 3). To the best of our knowledge, the crescent shaped lateral eye mass is a rare character in spiders. The elongated chelicera (more than 40 % of the length of carapace) is longer than in other species of the genus. The legs and spinnerets are also elongated, but males of Harmonicon audeae and Harmonicon oiapoqueae also have similar long legs. An additional possible troglomorphic character is the elongated trichobothria at the leg tarsus. In comparison to other Harmonicon species, the trichobothria of the new species are approximately twice as longer than the covering hairs and clearly visible in lateral view. Furthermore, Harmonicon cerberus sp. n. seems to bear more unequivocal troglomorphic characteristics compared to Linothele cavicola, the only other cave inhabiting Diplurinae (
This spider may be a troglobitic species, despite the small dimensions of Pequiá cave. Several other troglobitic species have been collected in some of the more than a thousand iron ore caves in Carajás (
Pequiá cave and its surroundings have been thoroughly investigated recently (Pedroso, pers. obs.). However, no additional specimens of Harmonicon have been found. On the other hand, only the entrance and the beginning of the lateral tube were investigated (around 1/3 of the cave), as there is a large water pool mixed with abundant guano. Unfortunately, the exploration of the terminal portion of the lateral tube of Pequiá cave needs special equipment, such as floaters. This portion appears to be the best candidate for new attempts to find additional specimens of Harmonicon cerberus sp. n., as it is farthest away from the mouth and the darkest area of the cave. Other possible areas for exploration are the numerous caves near Pequiá cave, specially Gavião cave, a larger cave situated circa 4 km from Pequiá cave (
Notes on Harmonicon. In their paper on the revalidation of Harmonicon,
The diagnosis of Harmonicon is not so clear-cut. Regarding the leg formula, the female of Harmonicon audeae itself, the species described by
Another inconsistent character is the absence of the prolateral knob of male metatarsus I. Both Harmonicon oiapoqueae and Harmonicon cerberus sp. n. present the cited knob, so its absence may be an autapomorphy of Harmonicon audeae.
Considering the lyra, the number and shape of the setae are not consistent throughout Diplurinae. Some Diplura species we have examined have lyra with just a few setae (down to 2), sometimes with tip curved and somewhat flattened. The setae tip though is not as curved and flattened as in Harmonicon. Again the number of setae is not a reliable character for Harmonicon, as already suggested by
Additional diagnostic characters for Harmonicon were proposed by
The presence of additional, rigid, and somewhat thickened, setae situated distally to the lyra (Fig. 6) may be diagnostic for Harmonicon or at least most of its species. Besides Harmonicon cerberus sp. n., these setae are found in specimens of Harmonicon rufescens from Altamira (unpublished data), Harmonicon oiapoqueae (see a picture on the website of
Some additional characters may prove to be diagnostic, at least in relation to Diplura. The longer tibia of the male pedipalp may distinguish Harmonicon from Diplura species, in which tibia are usually short and stout. Long and relatively thin tibia are found also in the diplurines Trechona (ex.
The legs and palp with denser and longer scopula, the longer tibia of the male palp and the flexible, highly cracked tarsi may indicate a closer relationship between Harmonicon, Trechona, and perhaps Linothele. Another common trait of these genera is the increased body size, compared to Diplura. Compared to Trechona, some easily seen diagnostic characters of Harmonicon are the lyra composed by less than ten setae (vs. complex lyra, with more than 50 setae in several layers) and the absence of the chevron pattern on the abdomen (vs. presence).
All the published records for described Harmonicon species are located in the northeastern Amazon region, from northern French Guiana to central Pará state, Brazil (Map 1). The type locality of Harmonicon cerberus sp. n. is the southernmost record for the genus, located in southern Pará state. However, we examined specimens of several Harmonicon species covering a much larger area, ranging from Peru in the west, to states in northeastern (Bahia, Ceará) and central (Mato Grosso, Goiás) regions in Brazil.
We are grateful to Gustavo Silva de Miranda (MNRJ) for help with the map and to Pedro de Souza Castanheira for help during the description process. This work was supported by a Ph.D. grant from Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ).