Research Article |
Corresponding author: Jose Fernandez-Triana ( cnc.braconidae@gmail.com ) Academic editor: Kees van Achterberg
© 2019 Jose Fernandez-Triana, Caroline Boudreault, Tanya Dapkey, M. Alex Smith, Winnie Hallwachs, Daniel Janzen.
This is an open access article distributed under the terms of the CC0 Public Domain Dedication.
Citation:
Fernandez-Triana J, Boudreault C, Dapkey T, Smith MA, Hallwachs W, Janzen D (2019) A revision of Dolichogenidea (Hymenoptera, Braconidae, Microgastrinae) with the second mediotergite broadly rectangular from Area de Conservación Guanacaste, Costa Rica. ZooKeys 835: 87-123. https://doi.org/10.3897/zookeys.835.33440
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The first species of Dolichogenidea (Hymenoptera: Braconidae, Microgastrinae) with the second mediotergite broadly quadrate to rectangular are revised, and eight new species from Area de Conservación Guanacaste (ACG), Costa Rica are described, all authored by Fernandez-Triana & Boudreault: alejandromasisi, angelagonzalezae, carlosmanuelrodriguezi, genuarnunezi, josealfredohernandezi, melaniamunozae, rogerblancoi, and yeimycedenoae. A new species group (carlosmanuelrodriguezi) within the genus is proposed to accommodate those species, as well as additional undescribed species from the Neotropical region found in collections. All new species are found in rainforests (120–900 m) and all are parasitoids of Depressariidae (except for one species parasitizing Choreutidae). The unique shape of the second mediotergite and long ovipositor are features shared with the alejandromorai species group in the genus Apanteles, an example of convergent evolution; both wasp groups also parasitize similar hosts in ACG.
Area de Conservación Guanacaste, DNA barcoding, Dolichogenidea, Microgastrinae, parasitoid biology, taxonomic revision
Dolichogenidea is a genus in the braconid subfamily Microgastrinae and contains almost 200 described species worldwide, with only four of them recorded from the Neotropics (Yu et al. 2016). However, there are hundreds of undescribed species in collections. For example, among the material from Costa Rica with DNA barcodes available (1,200+ species of Microgastrinae), there are more than 110 Dolichogenidea species, the great majority being undescribed (e.g., see DNA barcode data in
This study represents the first paper of a series dealing with Dolichogenidea from Area de Conservación Guanacaste (ACG) in northwestern Costa Rica. ACG contains 1,200 km2 of terrestrial conserved wildland dry forest, cloud forest and rain forest, extending from 0 to 2,000 m elevation continuously from the Pacific coast to the Caribbean lowlands; its biodiversity is being intensively inventoried (e.g.,
Below we describe eight new species which represent a unique group among known Dolichogenidea. They are characterized by a broadly quadrate to rectangular second mediotergite, a feature never previously reported among the described species of the genus.
The specimens studied for this paper are deposited in the: Canadian National Collection of Insects, Ottawa, Canada (
Morphological terms and measurements of structures follow those used by
The descriptions of the new species contain a general but brief account of color, sculpture, and morphological features and commonly used ratios in taxonomic studies of Microgastrinae. Raw measurements of morphological structures (in mm) are also provided, which would allow for additional ratios to be explored in the future. When presenting raw measurements, the holotype value is given first, followed by the range of other specimens of the same species between parentheses.
In the species descriptions, the holotype labels are detailed verbatim, with / separating the different lines of each label. For paratypes, specimen information was generated using the
We DNA barcoded some specimens, using the standard animal locus of the 5’ region of the cytochrome c oxidase I (COI) gene (Hebert et al. 2003). Briefly, DNA extracts were obtained from single legs using a glass fibre protocol (Ivanova et al. 2006), and total genomic DNA was re-suspended in 30 μl of dH2O. The barcode region (658 base pairs (bp) region near the 5’ terminus of the COI gene) was amplified using standard primers following established protocols (see
Photographs were taken with a Keyence VHX–1000 Digital Microscope, using a lens with a range of 10–130 ×. Multiple images were taken of a structure through the focal plane and then combined to produce a single in-focus image using the software associated with the Keyence System. Images were corrected using Adobe Photoshop CS4, plates were prepared using Microsoft PowerPoint 2010 and saved as .tiff files.
The genus Dolichogenidea was described by
The species described below are unique among all described Dolichogenidea. They can be easily distinguished from congeners by having the shape of T2 almost quadrate to broadly rectangular, and a relatively very long ovipositor and ovipositor sheaths. Because of their diagnostic value, both characters are described below with more details.
A perfectly quadrate T2 would have the same width at its posterior margin as its median length, i.e., the ratio between those two measurements would be 1.0 × (because anterior and posterior widths of T2 are usually different, the posterior width is the commonly used trait). The species dealt with here (Figs
All eight species described here have ovipositor sheaths 1.8–2.5 × as long as their metatibia lengths (Figs
Because of its uniqueness, we propose here this group to be named as the Dolichogenidea carlosmanuelrodriguezi species group, consisting of eight new species; they have all been found in rainforests (120–900 m) in ACG, and all except one species are parasitoids of Depressariidae (with a single wasp species parasitizing Choreutidae), with both solitary and gregarious species (Figs
Some of the new species described below are very similar morphologically. However, they are characterised by large interspecific molecular divergence within the DNA barcode region, with the interspecific distance ranging from 2.39 to 6.59 % (as compared with intraspecific distances between 0 and 0.61 %).
The species in the D. carlosmanuelrodriguezi group are surprisingly morphologically similar (regarding the shape of T2 and the comparatively long ovipositors) to the alejandromorai species group in the genus Apanteles (see
The patronyms for this group of Dolichogenidea are named in honor of the government team of Costa Rican biodiversity managers and administrators responsible for presenting the country to the 14th Conference of the Parties (COP 14) of the Convention for Biological Diversity, in October 2018.
Updated information on the hosts of the Apanteles alejandromorai and Dolichogenidea carlosmanuelrodriguezi species groups in ACG, Costa Rica. For some host species only interim names are available at present. For an explanation on how those interim names are produced see
Species | Host family (# of host species): host species names |
Apanteles | |
alejandromorai | Depressariidae (4): Antaeotricha Janzen 106; Antaeotricha Janzen 225; Antaeotricha Janzen 366; Lethata trochalosticta |
deifiliadavilae | Depressariidae (12): Antaeotricha marmorea; Antaeotricha radicalis; Antaeotricha Janzen86; Antaeotricha Janzen204; Antaeotricha radicalisDHJ01; Antaeotricha radicalisEPR03; Chlamydastis vitorbeckeri; Stenoma Janzen19; Stenoma Janzen20; Stenoma Janzen210; two unidentified Stenomatinae species with interim names “elachJanzen01 Janzen204” and “elachJanzen01 Janzen211” |
eulogiosequeirai | Depressariidae (1): Stenoma Janzen08 |
fernandochavarriai | Depressariidae (6): Antaeotricha BioLep46; Antaeotricha Janzen13; Antaeotricha Janzen31; Antaeotricha Janzen77; Antaeotricha Janzen290; Antaeotricha Janzen140DHJ01; Antaeotricha Phillips02 |
franciscoramirezi | Depressariidae (1): Antaeotricha Janzen727 |
freddysalazari | Depressariidae (2): Antaeotricha Janzen370; one unidentified Depressariinae species with interim name “elachJanzen01 Janzen227” |
gabrielagutierrezae | Depressariidae (2): Antaeotricha Janzen301; Antaeotricha Phillips01 |
juancarrilloi | Depressariidae (16): Antaeotricha renselariana; Antaeotricha stigmatiasDHJ01; Antaeotricha Janzen07; Antaeotricha Janzen09; Antaeotricha Janzen10; Antaeotricha Janzen39; Antaeotricha Janzen110; Antaeotricha Janzen146; Antaeotricha Janzen366; Lethata trochalosticta; Stenoma Janzen58; Stenoma Janzen99; Stenoma Janzen244; three unidentified Depressariinae species with interim names “elachBioLep01 BioLep709”; “elachBioLep01 Janzen48”; and “elachJanzen01 Janzen725” |
luisbrizuelai | Depressariidae (1): Antaeotricha Janzen128 |
luisgarciai | Depressariidae (1): unidentified Depressariinae species with interim name “elachJanzen01 Janzen301” |
marvinmendozai | Depressariidae (4): Antaeotricha BioLep54; Antaeotricha Janzen84; Antaeotricha Janzen146; Antaeotricha Janzen222 |
minornavarroi | Depressariidae (4): Antaeotricha renselariana; Antaeotricha Janzen146; Antaeotricha Janzen146; one unidentified Depressariinae species with interim name “elachJanzen01 Janzen120” |
tiboshartae | Depressariidae (16): Anadasmus Janzen16; Anadasmus Janzen25; Antaeotricha renselariana; Antaeotricha Janzen07; Antaeotricha Janzen09; Antaeotricha Janzen12; Antaeotricha Janzen86; Antaeotricha Janzen134; Antaeotricha Janzen146; Antaeotricha Janzen222; Antaeotricha Janzen228; Antaeotricha Janzen401; Antaeotricha radicalisDHJ01; Stenoma BioLep391; Stenoma Janzen144; one unidentified Depressariinae species with interim name “elachBioLep01 Janzen48” |
Dolichogenidea | |
alejandromasisi | Depressariidae (1): Antaeotricha renselariana |
angelagonzalezae | Choreutidae (1): Brenthia Janzen12 |
carlosmanuelrodriguezi | Depressariidae (1): Antaeotricha spurca |
genuarnunezi | Depressariidae (1): Antaeotricha phaeoneura |
josealfredohernandezi | Depressariidae (1): Stenoma Janzen99 |
melaniamunozae | Depressariidae (1): Cerconota recurvella |
rogerblancoi | Depressariidae (1): Antaeotricha radicalisDHJ01 |
yeimycedenoae | Depressariidae (1): Antaeotricha Janzen126 |
1 | Larger size (body L 4.80–4.90 mm; fore wing L 4.70–4.80 mm; ovipositor sheaths L 3.00–3.40 mm); metatrochanter and metatrochantellus entirely black [Hosts: Cerconota recurvella (Walker, 1864) Antaeotricha spurca (Zeller, 1855) (Depressariidae)] | Dolichogenidea melaniamunozae sp. n. |
– | Smaller size (body L less than 4.00 mm; fore wing L less than 3.70 mm; ovipositor sheaths L less than 3.10 mm); metatrochanter and/or metatrochantellus at least partially yellow | 2 |
2 | Mesofemur entirely yellow, metafemur almost entirely yellow (except for small, dark spot on anterior 0.1–0.2) | 3 |
– | Mesofemur partially dark brown (rarely mostly yellow with small, dark spots), metafemur mostly to entirely dark brown to black | 4 |
3 | Slightly smaller specimens (body L 3.70 mm; ovipositor sheaths 2.60 mm); T2 posterior W 2.0 × T2 L medially; F2 1.6 × as long as F14 [Host: Antaeotricha spurca (Zeller, 1855) (Depressariidae)] [A total of 21 DNA barcode diagnostic characters: 31T, 37A, 55G, 67A, 70A, 79A, 88A, 109A, 139A, 190C, 277A, 290G, 316G, 322G, 370T, 386T, 418T, 436T, 460A, 496G, 556A] | Dolichogenidea carlosmanuelrodriguezi sp. n. |
– | Slightly larger specimens (body L 3.90 mm; ovipositor sheaths 2.90 mm); T2 posterior W 1.8 × T2 L medially; F2 1.7–1.9 × as long as F14 [Host: Stenoma Janzen99] [A total of 21 DNA barcode diagnostic characters: 31A, 37G, 55A, 67T, 70G, 79T, 88T, 109G, 139T, 190A, 277T, 290A, 316A, 322T, 370A, 386A, 418A, 436A, 460G, 496A, 556GA] | Dolichogenidea josealfredohernandezi sp. n. |
4 | T1 comparatively broader, T1 L 1.5 × T1 anterior W, and T1 L 1.5 × T1 posterior W; T2 comparatively less quadrate (T2 posterior W 2.3 × T2 L medially); parasitizing caterpillars in the family Choreutidae [Host: Brenthia Janzen12] | Dolichogenidea angelagonzalezae sp. n. |
– | T1 comparatively narrower, T1 L 1.9–2.4 × (very rarely 1.6 ×) T1 anterior W, and T1 L 1.9–3.0 × (very rarely 1.6–1.7 × in small specimens) T1 posterior W; T2 comparatively more quadrate, T2 posterior W 1.3–1.9 × (very rarely 2.0–2.1 × in small specimens) T2 L medially; parasitizing caterpillars in the family Depressariidae | 5 |
5 | Mesofemur mostly yellow, at most with small, dark spots (if mesofemur mostly brown, then specimens significantly smaller, with body L 2.50–3.00 mm) | 6 |
– | Mesofemur mostly dark brown (body L at least 3.30 mm, usually more) | 7 |
6 | F2 2.1–2.5 × (average: 2.3 ×) as long as F14; T2 posterior W 1.3–1.9 × (average: 1.6 ×) T2 L medially; pterostigma L 3.0–3.5 × (average 3.2 ×) pterostigma width [Host: Antaeotricha renselariana (Stoll, [1781])] | Dolichogenidea alejandromasisi sp. n. |
– | F2 1.8–2.1 × (average: 1.9 ×) as long as F14; T2 posterior W 1.6–2.1 × (average: 1.8 ×) T2 L medially; pterostigma L 2.7–3.0 × (average 2.8 ×) pterostigma width [Host: Antaeotricha radicalisDHJ01] | Dolichogenidea rogerblancoi sp. n. |
7 | Pterostigma L 3.0 × pterostigma width; posterior ocellar line 1.6 × lateral ocellus diameter; ocular ocellar line 1.2 × posterior ocellar line [Host: Antaeotricha phaeoneura (Meyrick, 1913)] | Dolichogenidea genuarnunezi sp. n. |
– | Pterostigma L 3.1–3.3 × (average 3.2 ×) pterostigma width; posterior ocellar line 1.8–2.0 × lateral ocellus diameter; ocular ocellar line 1.2–1.5 × posterior ocellar line [Host: Antaeotricha Janzen126] | Dolichogenidea yeimycedenoae sp. n. |
Female, Costa Rica,
DHJPAR0035291.
Rio Blanco Abajo, 500 m, 10.90037N, -85.37254W, Sector San Cristobal, ACG, Alajuela province, Costa Rica.
Ten female and five male specimens mounted on individual points (
Dolichogenidea alejandromasisi can be recognized by its black to brown metafemur, comparatively narrower T1 (usually T1 L medially 2.0–3.0 × T1 posterior W), more quadrate T2 (T2 posterior W 1.30–1.90 × T2 L medially) and host being Depressariidae. D. rogerblancoi shares those features and is very similar morphologically, but it differs from alejandromasisi by having shorter F1 L, relatively less quadrate T2 (T2 posterior W 1.6–2.1 × T2 L medially) and relatively broader pterostigma. The two species parasitize different hosts (although in the same genus Antaeotricha), but alejandromasisi tends to be found at higher altitudes.
Body color: head and mesosoma black, metasoma black to dark brown; palpi, metatibial spines, tegula and most of humeral complex white-yellow; legs mostly orange-yellow, except for mesofemur (with small dark brown spots), metafemur (mostly brown), and apical 0.1–0.2 of metatibia and metatarsus brown; wing venation mostly white or transparent, except for fore wing veins R1, r, 2RS and 2M which are brown, pterostigma mostly brown but with small light spot at base. Anteromesoscutum mostly with setae and sculptured with punctures that do not fuse with each other; scutoscutellar sulcus relatively wide and with relatively deep crenulae; scutellar disc smooth and unsculptured, with isolated setae; propodeum mostly setose and with scattered punctures; propodeum areola partially defined on posterior half by longitudinal carinae, transverse carinae partially defined; T1 mostly smooth, with shallow and sparse punctures along lateral margins; T2+ smooth. Body Length: 3.66 (2.53–5.02). Fore wing L: 3.44 (2.55–3.80). Ovipositor sheaths L: 2.66 (1.98–2.90). F1 L: 0.26 (0.19–0.26). F2 L: 0.26 (0.19–0.27). F2 W: 0.07 (0.05–0.07). F3 L: 0.25 (0.19–0.27). F14 L: 0.11 (0.08–0.12). F14 W: 0.06 (0.04–0.06). F15 L: 0.10 (0.08–0.12). F16 L: 0.12 (0.10–0.14). Head height: 0.59 (0.48–0.60). Head width: 0.75 (0.62–0.80). Eye height: 0.39 (0.32–0.41). Malar distance: 0.11 (0.08–0.12). Mandible W: 0.12 (0.07–0.12). Ocular ocellar line: 0.13 (0.12–0.14). Posterior ocellar line: 0.13 (0.11–0.14). Lateral ocellar line: 0.08 (0.05–0.08). Scutellar disc L: 0.33 (0.26–0.35). Scutellar disc W at anterior margin: 0.32 (0.22–0.32). T1 L: 0.53 (0.38–0.58). T1 W at anterior margin: 0.33 (0.20–0.32). T1 W at posterior margin: 0.26 (0.20–0.28). T1 maximum width: 0.29 (0.22–0.33). T2 L: 0.20 (0.13–0.25). T2 W at anterior margin: 0.23 (0.18–0.25). T2 W at posterior margin: 0.28 (0.23–0.33). Metafemur L: 0.94 (0.68–0.94). Metafemur W: 0.28 (0.17–0.29). Metatibia L: 1.19 (0.84–1.30). Metatibial inner spur L: 0.32 (0.22–0.36). Metatibial outer spur L: 0.21 (0.12–0.23). Metatarsus first segment L: 0.67 (0.45–0.74). Pterostigma L: 0.70 (0.52–0.72). Pterostigma W: 0.21 (0.18–0.21). Fore wing vein R1 L: 0.83 (0.69–0.94). Fore wing vein r L: 0.26 (0.17–0.28). Fore wing vein 2RS L: 0.21 (0.14–0.21).
Reared from Antaeotricha renselariana (Stoll, [1781]) (Depressariidae). This is the only species of Dolichogenidea parasitizing that species of caterpillar in ACG, with 87 records out of 1,132 rearings.
Costa Rica, ACG, Sectores San Cristobal & Pitilla, 500–620 m. Rain forest ecosystem.
This species is represented in BOLD by 78 sequences, which belong to BIN BOLD:ABX6174.
Dolichogenidea alejandromasisi is dedicated to Alejandro Masis Cuevillas, Director of Area de Conservación Guanacaste, in recognition of his decades of protection and biodevelopment of the forests occupied by this wasp.
Female, Costa Rica,
DHJPAR0020711.
Vado Rio Francia, 400 m, 10.90093 –85.28915, Sector Rincon Rain Forest, ACG, Alajuela province, Costa Rica.
COSTA RICA: Alajuela, ACG, / Sector Rincon Rain Forest, / Vado Rio Francia, 400 m, / 10.90093N, -85.28915W, / 08/22/2007 / DHJPAR0020711.
One specimen of sex undetermined, from the same locality as holotype (
Dolichogenidea angelagonzalezae can be recognized by its black to brown metafemur, comparatively broader T1 (T1 L medially 1.5 × T1 posterior W), less quadrate T2 (T2 posterior W 2.3 × T2 L medially) and host being Choreutidae.
Body color: head and mesosoma black, metasoma black to dark brown; palpi, metatibial spines, tegula and most of humeral complex white-yellow; legs mostly orange-yellow, except for mesofemur (with small dark brown spots), metafemur (mostly brown), and apical 0.1–0.2 of metatibia and metatarsus brown; wing venation mostly white or transparent, except for fore wing veins R1, r, 2RS and 2M which are brown, pterostigma mostly brown but with small light spot at base. Anteromesoscutum mostly with setae and sculptured with punctures that do not fuse with each other; scutoscutellar sulcus relatively wide and with relatively deep crenulae; scutellar disc smooth and unsculptured, with isolated setae; propodeum mostly setose and with scattered punctures; propodeum areola partially defined on posterior half by longitudinal carinae, transverse carinae partially defined; T1 mostly smooth, with shallow and sparse punctures along lateral margins; T2+ smooth. Body Length: 3.72. Fore wing L: 3.88. Ovipositor sheaths L: 3.12. F1 L: 0.31. F2 L: 0.31. F2 W: 0.08. F3 L: 0.29. F14 L: 0.17. F14 W: 0.07. F15 L: 0.12. F16 L: 0.14. Head height: 0.61. Head width: 0.82. Eye height: 0.42. Malar distance: 0.11. Mandible W: 0.10. Ocular ocellar line: 0.14. Posterior ocellar line: 0.12. Lateral ocellar line: 0.08. Scutellar disc L: 0.39. Scutellar disc W at anterior margin: 0.32. T1 L: 0.50. T1 W at anterior margin: 0.33. T1 W at posterior margin: 0.32. T1 maximum width: 0.34. T2 L: 0.19. T2 W at anterior margin: 0.37. T2 W at posterior margin: 0.45. Metafemur L: 1.07. Metafemur W: 0.32. Metatibia L: 1.35. Metatibial inner spur L: 0.36. Metatibial outer spur L: 0.23. Metatarsus first segment L: 0.78. Pterostigma L: 0.74. Pterostigma W: 0.22. Fore wing vein R1 L: 0.91. Fore wing vein r L: 0.28. Fore wing vein 2RS L: 0.24.
Reared from Brenthia Janzen12 (Choreutidae). This is the only Microgastrinae known to parasitize that species of caterpillar in ACG, with three records out of four rearings, probably representing two sets of sibling caterpillars.
Costa Rica, ACG, Sector Rincon Rain Forest, 400–460 m. Rain forest ecosystem.
This species is represented in BOLD by three sequences (two full barcodes and one sequence 421 bp), which belong to BIN BOLD:AAL2298.
Dolichogenidea angelagonzalezae is dedicated to Angela Gonzalez Grau of Santo Domingo de Heredia, Costa Rica, in recognition of her dedication to supporting BioAlfa to render Costa Rica bioliterate, constructing CONAGEBIO (Comisión Nacional de Gestión de la Biodiversidad) as a biodiversity biodevelopment-friendly government agency, and for presenting Costa Rica’s Biodiversity Clearing House to COP14 in October 2018.
Female, Costa Rica,
DHJPAR0039047.
Sendero Rally, 821 m, 10.75993 -85.28738, Sector Santa Maria, ACG, Guanacaste province, Costa Rica.
COSTA RICA: Guanacaste, / ACG, Sector Santa Maria, / Sendero Rally, 821 m, / 10.75993N, -85.28738W, / 03/07/2010 / DHJPAR0039047.
Dolichogenidea carlosmanuelrodriguezi can be recognized by its legs almost entirely orange-yellow (except for small, dark spot on posterior 0.1–0.2 of metafemur). It is very similar to D. josealfredohernandezi, but the later species is slightly larger and with a slightly more quadrate T2 (although the variation of those characters is very small, to the point that both species are almost indistinguishable morphologically). However, they parasitize different host species, in different genera, and they also differ in a total of 21 DNA barcode diagnostic characters. Additionally, the two species have been found at different altitudes.
Body color: head and mesosoma black, metasoma black to dark brown; palpi, metatibial spines, tegula and most of humeral complex white-yellow; legs mostly orange-yellow, except for small, dark spot on posterior 0.1–0.2 of metafemur, and apical 0.1–0.2 of metatibia and metatarsus brown; wing venation mostly white or transparent, except for fore wing veins R1, r, 2RS and 2M which are brown, pterostigma mostly brown but with small light spot at base. Anteromesoscutum mostly with setae and sculptured with punctures that do not fuse with each other; scutoscutellar sulcus relatively wide and with relatively deep crenulae; scutellar disc smooth and unsculptured, with isolated setae; propodeum mostly setose and with scattered punctures; propodeum areola partially defined on posterior half by longitudinal carinae, transverse carinae partially defined; T1 mostly smooth, with shallow and sparse punctures along lateral margins; T2+ smooth. Body Length: 3.75. Fore wing L: 3.72. Ovipositor sheaths L: 2.62. F1 L: 0.29. F2 L: 0.28. F2 W: 0.09. F3 L: 0.28. F14 L: 0.17. F14 W: 0.07. F15 L: 0.12. F16 L: 0.15. Head height: 0.60. Head width: 0.84. Eye height: 0.42. Malar distance: 0.11. Mandible W: 0.11. Ocular ocellar line: 0.15. Posterior ocellar line: 0.12. Lateral ocellar line: 0.08. Scutellar disc L: 0.41. Scutellar disc W at anterior margin: 0.34. T1 L: 0.55. T1 W at anterior margin: 0.30. T1 W at posterior margin: 0.30. T1 maximum width: 0.32. T2 L: 0.18. T2 W at anterior margin: 0.32. T2 W at posterior margin: 0.36. Pterostigma L: 0.69. Pterostigma W: 0.23. Fore wing vein R1 L: 0.88. Fore wing vein r L: 0.30. Fore wing vein 2RS L: 0.22.
Reared from Antaeotricha spurca (Zeller, 1855) (Depressariidae). This is the only species of Dolichogenidea known to parasitize that species of caterpillar in ACG, with four records out of 344 rearings.
Costa Rica, ACG, Sector Santa Maria, 820 m. Rain forest ecosystem.
This species is represented in BOLD by four sequences which belong to BIN BOLD:ABZ4155. Interestingly, that BIN may contain more than carlosmanuelrodriguezi, as four other undescribed species (with interim names Janzen51, Janzen156, Janzen 251, and Janzen325) are also included within BOLD:ABZ4155. We have not been able to examine those specimens, and so while it is possible that they are all the same species, we doubt it owing to their different host records. Resolving this potential complex of cryptic species will require future examination of more specimens and is beyond the scope of the present paper.
Dolichogenidea carlosmanuelrodriguezi is dedicated to Carlos Manuel Rodriguez of San Jose, Costa Rica, Minister of Environment & Energy (MINAE), in recognition of his life-long support of ACG and Costa Rican biodiversity conservation overall, as well as being today’s Costa Rican representative for BioAlfa and other biodiversity conservation initiatives for the global Convention for Biological Diversity.
Female, Costa Rica,
DHJPAR0050092.
Rio Blanco Abajo, 500 m, 10.90037N, -85.37254W, Sector San Cristobal, ACG, Alajuela province, Costa Rica.
COSTA RICA: Alajuela, ACG, / Sector San Cristobal, / Rio Blanco Abajo, 500 m, / 10.90037N, -85.37254W, / 09/22/2012 / DHJPAR0050092.
Dolichogenidea genuarnunezi can be recognized by its mesofemur mostly dark brown, comparatively narrower T1 (T1 L medially more than 2.0 × T1 posterior W), relatively more quadrate T2 (T2 posterior W 1.6 × T2 L medially) and host being Depressariidae. The mesofemur color would separate this species from D. alejandromasisi and D. rogerblancoi. However, D. genuarnunezi is very similar to D. yeimycedenoae, with only slight differences in pterostigma L/W ratio, as well as proportions of posterior ocellar line, lateral ocellus diameter and ocular ocellar line (see key above for details). The variation of those characters is very small, to the point that both species are very similar morphologically. However, they parasitize different host species, and also differ significantly molecularly (available DNA barcodes are 4.54% different). Additionally, the two species have been found at different altitudes.
Body color: head and mesosoma black, metasoma black to dark brown; palpi, metatibial spines, tegula and most of humeral complex white-yellow; legs mostly orange-yellow, except for mesofemur and metafemur mostly brown, and apical 0.1–0.2 of metatibia and metatarsus brown; wing venation mostly white or transparent, except for fore wing veins R1, r, 2RS and 2M which are brown, pterostigma mostly brown but with small light spot at base. Anteromesoscutum mostly with setae and sculptured with punctures that do not fuse with each other; scutoscutellar sulcus relatively wide and with relatively deep crenulae; scutellar disc smooth and unsculptured, with isolated setae; propodeum mostly setose and with scattered punctures; propodeum areola partially defined on posterior half by longitudinal carinae, transverse carinae partially defined; T1 mostly smooth, with shallow and sparse punctures along lateral margins; T2+ smooth. Body Length: 3.78. Fore wing L: 3.44. Ovipositor sheaths L: 2.74. F1 L: 0.27. F2 L: 0.28. F2 W: 0.09. F3 L: 0.28. F14 L: 0.13. F14 W: 0.07. F15 L: 0.12. F16 L: 0.15. Head height: 0.58. Head width: 0.77. Eye height: 0.39. Malar distance: 0.10. Mandible W: 0.10. Ocular ocellar line: 0.14. Posterior ocellar line: 0.12. Lateral ocellar line: 0.08. Scutellar disc L: 0.36. Scutellar disc W at anterior margin: 0.31. T1 L: 0.62. T1 W at anterior margin: 0.29. T1 W at posterior margin: 0.25. T1 maximum width: 0.32. T2 L: 0.21. T2 W at anterior margin: 0.22. T2 W at posterior margin: 0.33. Metafemur L: 0.96. Metafemur W: 0.26. Metatibia L: 1.22. Metatibial inner spur L: 0.32. Metatibial outer spur L: 0.20. Metatarsus first segment L: 0.64. Pterostigma L: 0.73. Pterostigma W: 0.24. Fore wing vein R1 L: 0.85. Fore wing vein r L: 0.27. Fore wing vein 2RS L: 0.22.
Reared from Antaeotricha phaeoneura (Meyrick, 1913) (Depressariidae). This is the only species of Dolichogenidea known to parasitize that species of caterpillar in ACG, with one record out of 25 rearings.
Costa Rica, ACG, Sector San Cristobal, 500m. Rain forest ecosystem.
This species is represented in BOLD by one sequence belonging to BIN BOLD:ACC1300.
Dolichogenidea genuarnunezi is dedicated to Genuar Roman Núñez Vega of Guadalupe, Costa Rica, in recognition of his dedication to supporting BioAlfa to render Costa Rica bioliterate, constructing CONAGEBIO as a biodiversity biodevelopment-friendly government agency, and constructing Costa Rica’s Biodiversity Clearing House for COP14 of the Convention for Biological Diversity in October 2018.
Female, Costa Rica,
DHJPAR0049909.
Sendero Anonas, 405 m, 10.90528N, -85.27882W, Sector Rincon Rain Forest, ACG, Alajuela province, Costa Rica.
COSTA RICA: Alajuela, ACG, / Sector Rincon Rain Forest, / Sendero Anonas, 405 m, / 10.90528N, -85.27882W, / 08/04/2012 / DHJPAR0049909.
One female (
Dolichogenidea josealfredohernandezi can be recognized by its legs almost entirely orange-yellow (except for small, dark spot on posterior 0.1–0.2 of metafemur). It is very similar to D. carlosmanuelrodriguezi, but the later species is slightly smaller and with a slightly less quadrate T2 (although the variation of those characters is very small, to the point that both species are almost indistinguishable morphologically). However, they parasitize different host species, in different genera, and they also differ in a total of 21 DNA barcode diagnostic characters. Additionally, the two species have been found at different altitudes.
Body color: head and mesosoma black, metasoma black to dark brown; palpi, metatibial spines, tegula and most of humeral complex white-yellow; legs mostly orange-yellow, except for small, dark spot on posterior 0.1–0.2 of metafemur, and apical 0.1–0.2 of metatibia and metatarsus brown; wing venation mostly white or transparent, except for fore wing veins R1, r, 2RS and 2M which are brown, pterostigma mostly brown but with small light spot at base. Anteromesoscutum mostly with setae and sculptured with punctures that do not fuse with each other; scutoscutellar sulcus relatively wide and with relatively deep crenulae; scutellar disc smooth and unsculptured, with isolated setae; propodeum mostly setose and with scattered punctures; propodeum areola partially defined on posterior half by longitudinal carinae, transverse carinae partially defined; T1 mostly smooth, with shallow and sparse punctures along lateral margins; T2+ smooth. Body Length: 3.94 (3.92). Fore wing L: 3.72 (3.80). Ovipositor sheaths L: 2.88 (2.90). F1 L: 0.30 (0.32). F2 L: 0.30 (0.30). F2 W: 0.09 (0.10). F3 L: 0.29 (0.30). F14 L: 0.16 (0.18). F14 W: 0.07 (0.07). F15 L: 0.11 (0.13). F16 L: 0.13 (0.14). Head height: 0.60 (0.59). Head width: 0.80 (0.81). Eye height: 0.41 (0.41). Malar distance: 0.14 (0.11). Mandible W: 0.08 (0.11). Ocular ocellar line: 0.14 (0.15). Posterior ocellar line: 0.13 (0.13). Lateral ocellar line: 0.06 (0.08). Scutellar disc L: 0.36 (0.38). Scutellar disc W at anterior margin: 0.37 (0.39). T1 L: 0.62 (0.62). T1 W at anterior margin: 0.32 (0.31). T1 W at posterior margin: 0.32 (0.33). T1 maximum width: 0.37 (0.34). T2 L: 0.20 (0.22). T2 W at anterior margin: 0.29 (0.32). T2 W at posterior margin: 0.37 (0.40). Metafemur L: 1.04 (1.07). Metafemur W: 0.32 (0.33). Metatibia L: 1.32 (1.36). Metatibial inner spur L: 0.36 (0.36). Metatibial outer spur L: 0.22 (0.22). Metatarsus first segment L: 0.75 (0.81). Pterostigma L: 0.72 (0.72). Pterostigma W: 0.22 (0.23). Fore wing vein R1 L: 0.88 (0.93). Fore wing vein r L: 0.32 (0.23). Fore wing vein 2RS L: 0.18 (0.20).
Reared from Stenoma Janzen99 (Depressariidae). This is the only species of Dolichogenidea known to parasitize that species of caterpillar in ACG, with seven records out of 126 rearings.
Costa Rica, ACG, Sector Rincon Rain Forest, 400m. Rain forest ecosystem.
This species is represented in BOLD by 5 sequences belonging to BIN BOLD:ABA9255.
Dolichogenidea josealfredohernandezi is dedicated to José Alfredo Hernández of Alajuela, Costa Rica in recognition of his dedication to supporting BioAlfa to render Costa Rica bioliterate, representing CONAGEBIO and Costa Rican membership in iBOL (=BioScan at http://ibol.org/site/), and supporting conservation biodevelopment of ACG.
Dolichogenidea josealfredohernandezi is very similar to D. carlosmanuelrodriguezi and we have not yet located a good morphological character to distinguish them. However, they parasitize quite different hosts on very different plants and their DNA barcodes are also very different (3.4% base pairs).
Female, Costa Rica,
DHJPAR0051857.
Selva, 410 m, 10.92291 -85.31877, Sector Rincon Rain Forest, ACG, Alajuela province, Costa Rica.
Six females (
This species can be recognized by its larger body size, fore wing L and ovipositor sheaths (as compared to all other described species in this group), and also because of its entirely black metatrochanter and metatrochantellus.
Body color: head and mesosoma black, metasoma black to dark brown; palpi, metatibial spines, tegula and most of humeral complex white-yellow; legs mostly orange-yellow, except for mesofemur and metafemur mostly to entirely dark brown or black, and apical 0.3 of metatibia and metatarsus dark brown to black; wing venation mostly white or transparent, except for fore wing veins R1, r, 2RS and 2M which are brown, pterostigma mostly brown but with small light spot at base. Anteromesoscutum mostly with setae and sculptured with punctures that do not fuse with each other; scutoscutellar sulcus relatively wide and with relatively deep crenulae; scutellar disc smooth and unsculptured, with isolated setae; propodeum mostly setose and with scattered punctures; propodeum areola partially defined on posterior half by longitudinal carinae, transverse carinae partially defined; T1 mostly smooth, with shallow and sparse punctures along lateral margins; T2+ smooth. Body Length: 4.80 (4.48–4.90). Fore wing L: 4.80 (4.72–4.80). Ovipositor sheaths L: 3.44 (3.00–3.44). F1 L: 0.39 (0.37–0.40). F2 L: 0.37 (0.36–0.37). F2 W: 0.12 (0.10–0.12). F3 L: 0.36 (0.34–0.37). F14 L: 0.20 (0.18–0.20). F14 W: 0.08 (0.07). F15 L: 0.13 (0.13–0.15). F16 L: 0.18 (0.15–0.17). Head height: 0.68 (0.67–0.69). Head width: 0.96 (0.92–0.96). Eye height: 0.48 (0.47–0.48). Malar distance: 0.10 (0.10–0.12). Mandible W: 0.12 (0.11–0.12). Ocular ocellar line: 0.17 (0.16–0.17). Posterior ocellar line: 0.15 (0.15–0.17). Lateral ocellar line: 0.08 (0.08). Scutellar disc L: 0.53 (0.51–0.52). Scutellar disc W at anterior margin: 0.42 (0.42–0.43). T1 L: 0.69 (0.72–0.82). T1 W at anterior margin: 0.28 (0.37–0.45). T1 W at posterior margin: 0.41 (0.40–0.43). T1 maximum width: 0.46 (0.45–0.48). T2 L: 0.26 (0.21–0.26). T2 W at anterior margin: 0.43 (0.41–0.46). T2 W at posterior margin: 0.63 (0.43–0.61). Metafemur L: 1.25 (1.26–1.31). Metafemur W: 0.38 (0.37–0.40). Metatibia L: 1.60 (1.54–1.68). Metatibial inner spur L: 0.42 (0.40–0.45). Metatibial outer spur L: 0.25 (0.24–0.26). Metatarsus first segment L: 0.98 (0.93–0.98). Pterostigma L: 0.88 (0.86–0.89). Pterostigma W: 0.25 (0.26–0.28). Fore wing vein R1 L: 1.08 (0.99–1.05). Fore wing vein r L: 0.36 (0.33–0.39). Fore wing vein 2RS L: 0.22 (0.22–0.26).
Reared from Cerconota recurvella (Walker, 1864) (Depressariidae). This is the only species of Dolichogenidea known to parasitize that species of caterpillar in ACG, with 38 records out of 521 reared caterpillars of this species.
Costa Rica, ACG, Sector Rincon Rain Forest, 135–540 m. Rain forest ecosystem.
This species is represented in BOLD by 35 sequences belonging to BIN BOLD:AAB5701.
Dolichogenidea melaniamunozae is dedicated to Melania Muñoz García of San Jose, Costa Rica, in recognition of her dedication to the CONAGEBIO team of biodiversity administrators and her enthusiasm for understanding GDFCF (Guanacaste Dry Forest Conservation Fund) and ACG efforts to do conservation through biodiversity development.
Female, Costa Rica,
DHJPAR0049840.
Finca Esmeralda, 123 m, 10.93548N, -85.25314W, Sector Rincon Rain Forest, ACG, Alajuela province, Costa Rica.
COSTA RICA: Alajuela, / ACG, Sector Rincon Rain Forest, / Finca Esmeralda, 123 m, / 10.93548N, -85.25314W, / 06/24/2012 / DHJPAR0049840.
Twelve female and four male specimens (
Dolichogenidea rogerblancoi can be recognized by its black to brown metafemur, comparatively narrower T1 (usually with T1 L medially 2.0–3.0 × T1 posterior W), more quadrate T2 (T2 posterior W 1.6–2.1 × T2 L medially) and host being Depressariidae. D. alejandromasisi shares those features and is very similar morphologically, but it differs from rogerblancoi by having longer F1 L, relatively more quadrate T2 (T2 posterior W 1.3–1.9 × T2 L medially) and relatively narrower pterostigma. The two species parasitize different hosts (although in the same genus Antaeotricha), but rogerblancoi tends to be found at lower altitudes.
Body color: head and mesosoma black, metasoma black to dark brown; palpi, metatibial spines, tegula and most of humeral complex white-yellow; legs mostly orange-yellow, except for mesofemur (with small dark brown spots), metafemur (mostly brown), and apical 0.1–0.2 of metatibia and metatarsus brown; wing venation mostly white or transparent, except for fore wing veins R1, r, 2RS and 2M which are light brown, pterostigma mostly brown but with small light spot at base. Anteromesoscutum mostly with setae and sculptured with punctures that do not fuse with each other; scutoscutellar sulcus relatively wide and with relatively deep crenulae; scutellar disc smooth and unsculptured, with isolated setae; propodeum mostly setose and with scattered punctures; propodeum areola partially defined on posterior half by longitudinal carinae, transverse carinae partially defined; T1 mostly smooth, with shallow and sparse punctures along lateral margins; T2+ smooth. Body L: 3.44 (2.73–3.84). Fore wing L: 3.41 (2.60–3.47). Ovipositor sheaths L: 2.94 (1.36–3.12). F1 L: 0.27 (0.18–0.29). F2 L: 0.26 (0.19–0.28). F2 W: 0.08 (0.06–0.08). F3 L: 0.26 (0.19–0.27). F14 L: 0.14 (0.10–0.14). F14 W: 0.06 (0.05–0.07). F15 L: 0.11 (0.08–0.12). F16 L: 0.14 (0.11–0.15). Head height: 0.58 (0.50–0.59). Head width: 0.77 (0.62–0.82). Eye height: 0.38 (0.32–0.40). Malar distance: 0.10 (0.09–0.12). Mandible W: 0.10 (0.08–0.11). Ocular ocellar line: 0.15 (0.13–0.15). Posterior ocellar line: 0.12 (0.10–0.12). Lateral ocellar line: 0.08 (0.05–0.08). Scutellar disc L: 0.34 (0.25–0.37). Scutellar disc W at anterior margin: 0.31 (0.20–0.29). T1 L: 0.65 (0.38–0.63). T1 W at anterior margin: 0.27 (0.22–0.32). T1 W at posterior margin: 0.26 (0.20–0.28). T1 maximum width: 0.28 (0.23–0.33). T2 L: 0.21 (0.14–0.22). T2 W at anterior margin: 0.27 (0.21–0.27). T2 W at posterior margin: 0.36 (0.29–0.38). Metafemur L: 0.98 (0.67–0.99). Metafemur W: 0.27 (0.17–0.27). Metatibia L: 1.22 (0.86–1.24). Metatibial inner spur L: 0.32 (0.22–0.34). Metatibial outer spur L: 0.18 (0.14–0.22). Metatarsus first segment L: 0.70 (0.48–0.73). Pterostigma L: 0.72 (0.54–0.71). Pterostigma W: 0.24 (0.19–0.25). Fore wing vein R1 L: 0.86 (0.68–0.88). Fore wing vein r L: 0.25 (0.17–0.26). Fore wing vein 2RS L: 0.20 (0.13–0.21).
Reared from Antaeotricha radicalisDHJ01 (Depressariidae). This is the only species of Dolichogenidea known to parasitize that species of caterpillar in ACG, with 24 records out of 243 reared caterpillars of this species. It does not parasitize any of the other ACG species of the “Antaeotricha radicalis” species complex.
Costa Rica, ACG, Sectores San Cristobal & Rincon Rain Forest, 123–670 m. Rain forest ecosystem.
This species is represented in BOLD by 22 sequences belonging to BIN BOLD:AAL2325.
Dolichogenidea rogerblancoi is dedicated to Roger Blanco Segura of Area de Conservación Guanacaste, co-coordinator of ACG Research & Subdirector of ACG Area Silvestre Protegido, in recognition of his many decades of protection and biodevelopment of the forests occupied by this wasp.
Female, Costa Rica,
DHJPAR0054623.
Sendero Orosilito, 900 m, 10.98332N, -85.43623W, Sector Pitilla, ACG, Guanacaste province, Costa Rica.
COSTA RICA: Guanacaste, / ACG, Sector Pitilla, / Sendero Orosilito, 900 m, / 10.98332N, -85.43623W, / 11/06/2013 / 13–SRNP–31589.
Four females (
Dolichogenidea yeimycedenoae can be recognized by its mesofemur mostly dark brown, comparatively narrower T1 (T1 L medially more than 2.5 × T1 posterior W), relatively more quadrate T2 (T2 posterior W 1.5 × T2 L medially) and host being Depressariidae. The mesofemur color would separate this species from D. alejandromasisi and D. rogerblancoi. However, D. yeimycedenoae is very similar morphologically to D. genuarnunezi, with only slight differences in pterostigma L/W, as well as proportions of posterior ocellar line, lateral ocellus diameter and ocular ocellar line (see key above for details). The variation of those characters is very small, to the point that both species are very similar morphologically. However, they parasitize different host species, and also differ significantly molecularly (available DNA barcodes are 4.54% different). Additionally, the two species have been found at different altitudes.
Body color: head and mesosoma black, metasoma black to dark brown; palpi, metatibial spines, tegula and most of humeral complex white-yellow; legs mostly orange-yellow, except for mesofemur and metafemur mostly brown, and apical 0.1–0.2 of metatibia and metatarsus brown; wing venation mostly white or transparent, except for fore wing veins R1, r, 2RS and 2M which are brown, pterostigma mostly brown but with small light spot at base. Anteromesoscutum mostly with setae and sculptured with punctures that do not fuse with each other; scutoscutellar sulcus relatively wide and with relatively deep crenulae; scutellar disc smooth and unsculptured, with isolated setae; propodeum mostly setose and with scattered punctures; propodeum areola partially defined on posterior half by longitudinal carinae, transverse carinae partially defined; T1 mostly smooth, with shallow and sparse punctures along lateral margins; T2+ smooth. Body Length: 3.96 (3.31–4.25). Fore wing L: 3.53 (3.44–3.75). Ovipositor sheaths L: 3.00 (2.28–3.19). F1 L: 0.28 (0.26–0.28). F2 L: 0.31 (0.28–.029). F2 W: 0.07 (0.07–0.08). F3 L: 0.27 (0.25–0.28). F14 L: 0.15 (0.12–0.17). F14 W: 0.06 (0.06–0.08). F15 L: 0.12 (0.10–0.12). F16 L: 0.16 (0.12–0.15). Head height: 0.58 (0.58–0.61). Head width: 0.77 (0.73–0.79). Eye height: 0.39 (0.36–0.39). Malar distance: 0.11 (0.09–0.11). Mandible W: 0.11 (0.08–0.12). Ocular ocellar line: 0.18 (0.17–0.18). Posterior ocellar line: 0.12 (0.13–0.14). Lateral ocellar line: 0.07 (0.07–0.08). Scutellar disc L: 0.35 (0.34–0.38). Scutellar disc W at anterior margin: 0.32 (0.28–0.32). T1 L: 0.60 (0.57–0.66). T1 W at anterior margin: 0.28 (0.29–0.30). T1 W at posterior margin: 0.22 (0.22–0.25). T1 maximum width: 0.27 (0.29–0.32). T2 L: 0.22 (0.18–0.21). T2 W at anterior margin: 0.31 (0.28–0.30). T2 W at posterior margin: 0.33 (0.34–0.38). Metafemur L: 1.05 (0.96–1.18). Metafemur W: 0.27 (0.25–0.31). Metatibia L: 1.25 (1.19–1.34). Metatibial inner spur L: 0.33 (0.31–0.32). Metatibial outer spur L: 0.17 (0.18–0.20). Metatarsus first segment L: 0.76 (0.70–0.77). Pterostigma L: 0.70 (0.67–0.82). Pterostigma W: 0.22 (0.21–0.25). Fore wing vein R1 L: 0.88 (0.83–0.85). Fore wing vein r L: 0.28 (0.22–0.27). Fore wing vein 2RS L: 0.18 (0.17–0.21).
Reared from Antaeotricha Janzen126 (Depressariidae). This is the only species of Dolichogenidea known to parasitize that species of caterpillar in ACG, with one record out of three reared caterpillars of this species.
Costa Rica, ACG, Sectores Pitilla & San Cristobal, 722–900 m. Rain forest ecosystem.
This species is represented in BOLD by 1 sequences belonging to BIN BOLD:ABY3724.
Dolichogenidea yeimycedenoi is dedicated to Yeimy Cedeño Solis of Moravia & Ostional, Costa Rica, in recognition of her dedication to understanding and explaining the BioAlfa project to render Costa Rica bioliterate, to COP14 of the Convention for Biological Diversity in October 2018.
The holotype is a teneral specimen, and thus its coloration (as shown in Fig.
A neighbour-joining tree representing the interspecific variation in the DNA barcode region for the holotypes of the Dolichogenidea species described in this paper. The tree was built in MEGA X (
We emphatically and gratefully acknowledge the support of the ACG parataxonomist team (