Research Article |
Corresponding author: Rafał Gosik ( cossonus@gmail.com ) Academic editor: Miguel Alonso-Zarazaga
© 2019 Rafał Gosik, Peter Sprick, Michael G. Morris.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Gosik R, Sprick P, Morris MG (2019) Descriptions of immature stages of four species of the genera Graptus, Peritelus, Philopedon, and Tanymecus and larval instar determination in Tanymecus (Coleoptera, Curculionidae, Entiminae). ZooKeys 813: 111-150. https://doi.org/10.3897/zookeys.813.30336
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The mature larva and pupa of Graptus triguttatus triguttatus and the mature larva of Peritelus sphaeroides are described for the first time. The larvae of Philopedon plagiatum and Tanymecus palliatus are re-described. Five larval instars were determined in Tanymecus, thereby correcting doubtful data in the literature. The relationship between larval growth, number of larval instars, head width of the mature larva, and the adult weevil is explained using the example of Tanymecus. The nearly constant ratio of subsequent larval instars in head width ratio, termed “growth factor” and derived from Dyar’s ratio, is used for the determination of larval instars. Larval collecting and breeding data are discussed in relation to their significance for the clarification of life-cycles.
Bionomics, Central European region, chaetotaxy, Curculionoidea , Entiminae , immature stages, larval instar determination, morphology, taxonomy, weevils
In this continued contribution on larvae of the subfamily Entiminae Schönherr, 1823 we describe or redescribe the mature larvae of four further species (e.g.,
In the present paper we describe for the first time the mature larva and pupa of Graptus triguttatus (Fabricius, 1775). For this species,
From the tribe Peritelini we describe the mature larva of Peritelus sphaeroides Germar, 1824 for the first time. The pupa was already described by
Despite the frequency and abundance of Tanymecus palliatus (Fabricius, 1787), and the good characterization of its development (
The aim of this paper is to describe the mature larvae of the four Entiminae species mentioned before and to give some examples about how to use data from larval descriptions for the determination of larval instars and for the study of life-cycles. An important prerequisite for studying life-cycles is to have correctly identified larvae, which is often difficult and a main reason why life-cycles of Entiminae weevils, apart from some noxious Otiorhynchus and Sitona species, are usually little known.
Specimens of three of the four species studied were collected in the field under certain plants and usually at the same sites where adults were previously collected. Larval instars of the fourth species, Peritelus sphaeroides Germar, 1824, were obtained in captivity by breeding in an air-conditioned room (see
All specimens studied were fixed in 75% ethanol and examined under an optical stereomicroscope (Olympus SZ 60 and SZ11) with calibrated oculars. Measurements of larval instars were made for: body length (BL), body width (BW) (usually at abdominal segment I or II), width (HW) and height (HH) of the head capsule (see Fig.
The observations and measurements were conducted using a light compound microscope with calibrated oculars. Drawings and outlines were made using a drawing tube (MNR–1) installed on a stereomicroscope (Ampliwal) and processed by computer software (Corel Photo-Paint X7, Corel Draw X7). Photos were taken with an Olympus E-M10 or using an Olympus BX63 microscope and processed by Olympus cellSens Dimension software. The larvae selected for pictures using SEM (scanning electron microscope) were at first dried in absolute ethyl alcohol (99.8%), rinsed in acetone, treated by CPD procedure (critical point drying) and then gold-plated. For the examination of selected structures a TESCAN Vega 3 SEM was used. General terminology and chaetotaxy follow
Morphological abbreviations:
Abd. 1–10 – abdominal segments 1–10, Th. 1–3 – thoracic segments 1–3, at – antenna, clss – clypeal sensorium, st – stemmata, Se – sensorium, sa – sensillum ampullaceum, sb – sensillum basiconicum, lr – labral rods, ur – urogomphus; setae: als – anterolateral, ams – anteromedial, as – alar (larva), as – apical (pupa), cls – clypeal, d – dorsal, des – dorsal epicranial, dms – dorsal malar, ds – discal (pupa), ds – dorsal (larva), eps – epipleural, es – epistomal, eus – eusternal, fes – femoral, fs – frontal, les – lateral epicranial, ligs – ligular, lrs – labral, ls – lateral, lsts – laterosternal, mbs – malar basiventral, mds – mandibular, mes – median, mps – maxillary palp, os – orbital, pas – postantennal, pda – pedal, pds – postdorsal, pls – posterolateral, pes – postepicranial, pfs – palpiferal, pms – postlabial, prms – prelabial, prns – pronotal, prs – prodorsal, ps – pleural, rs – rostral, sos – superorbital, ss – spiracular, stps – stipal, sts – sternal, ts – terminal, v – ventral (pupa), ves – ventral (larva), vms – ventral malar, vs – vertical.
We follow
All these specimens are deposited in the collection of the Department of Zoology, Maria Curie-Skłodowska University (Lublin, Poland). In Table
Larval instar determination is based on Dyar’s law (
5 premature larvae: Germany, Niedersachsen, Braunschweig, apple orchard and meadow in the area of Julius-Kühn-Institut (JKI), all under Plantago lanceolata L.; 10.01.2013: 3 ex., 18.04.2013: 1 ex., 24.05.2013: 1 ex.
22 mature and penultimate instar larvae: same site as previously noted, 10.1.2013: 5 ex., 18.04.2013: 7 ex., 03.05.2013: 7 ex., 24.05.2013: 1 ex., 10.06.2013: 2 ex.
3 pupae: Germany, Niedersachsen, Braunschweig, JKI, apple orchard, meadow; 2 mature larvae, collected on 24.05. and 10.06.2013, both had developed into the pupal stage in breeding boxes in the laboratory in Hannover on 20.06.2013. Another mature larva from the same site, collected on 03.05.2013, developed into the pupal stage before 27.05.2013.
Body length: 6.8–8.5 mm, body width: 3.0–3.4 mm, head width: 1.53–1.55 mm, head height: 1.35–1.37 mm.
Body (Figs
Graptus triguttatus mature larva. 1 Habitus 2 Last abdominal segments lateral view 3 Last abdominal segments ventral view Peritelus sphaeroides mature larva. 4 Habitus 5 Last abdominal segments lateral view 6 Last abdominal segments ventral view Philopedon plagiatum mature larva 7 Habitus 8 Last abdominal segments lateral view 9 Last abdominal segments ventral view Tanymecus palliatus mature larva 10 Habitus 11 Last abdominal segments lateral view 12 Last abdominal segments ventral view.
Graptus triguttatus triguttatus mature larva, habitus and chaetotaxy. 13 Thoracic segments, lateral view 14 First abdominal segment lateral view 15 Abdominal segments 7–10 lateral view 16 Abdominal segments 7–10 ventral view 17 Abdominal segments 7–10 dorsal view. Abbreviations: Th. 1–3 – thoracic segments 1–3, Abd. 1–10 – abdominal segments 1–10, setae: as – alar, ps – pleural, eps – epipleural, ds – dorsal, lsts – laterosternal, eus – eusternal, pda – pedal, pds – postdorsal, prns – pronotal, prs – prodorsal, ss – spiracular, sts – sternal, ts – terminal.
Head (Fig.
Graptus triguttatus triguttatus mature larva, body parts. 22 Right maxilla apical part ventral aspect 23 Right maxilla apical part dorsal aspect 24 Maxillolabial complex ventral aspect. Abbreviations: dms – dorsal malar, ligs – ligular, mbs – malar basiventral, mps – maxillary palp, pfs – palpiferal, prms – prelabial, pms – postlabial, stps – stipal, vms – ventral malar.
Body length (♂, ♀): 7.5–9.0 mm; body width (at level of mesocoxae): 3.8–4.5 mm; width of thorax: 2.0–2.3 mm.
Body moderately slender, straight, yellowish. Cuticle smooth (Figs
Graptus triguttatus triguttatus pupa. 25 Ventral 26 Dorsal 27 Lateral view. Abbreviations: Th. 1–3 – pro–, meso– and metathorax, Abd. 1–9 – abdominal segments 1–9, ur – urogomphus, setae: as – apical, d – dorsal, ds – discal, es – epistomal, fes – femoral, l, ls – lateral, os – orbital, pas – postantennal, pls – posterolateral, rs – rostral, sos – superorbital, v – ventral, vs – vertical.
Chaetotaxy well developed, setae variable in lengthand shape: spine-like or capilliform, dark yellow to brown, usually located on visible protuberances. Head capsule and rostrum include 1 vs, 2 minute sos, 1 spine-like and 1 minute os, 2 pas, 3 rs of varied sizes and 1 minute es. Except sos and es, all setae of the head and rostrum are placed on protuberances. Pronotum with 2 as, 1 ls, 2 ds and 2 pls. All setae of pronotum spine-like, of equal size (only ds1 slightly larger than others); all setae placed on protuberances. Mesothorax with 2 minute setae placed anteromedially and 3 spine-like setae placed medially. Metathorax with 4 spine-like setae placed medially. Abdominal segments 1–7 with 7 pairs of d1–7: d1–6 short, spine-like, placed on protuberances, in lines along the posterior margin of segments, d7 short, capilliform, placed anterolaterally, and 2 minute l1–2. Setae no. 3 and no. 5 increasing gradually from segment 2 to 7. Segment 8 with 4 pairs of spine-like setae of varied lengths (d1–4), placed on protuberances, in lines along the posterior margin of the segment. Seta no. 2 distinctly larger than others. Segment 9 with 3 pairs of short, capilliform v1–3. Each apex of femora with 2 fes, spine-like and of various length.
Rearing was started on 02.05.2012 in the climate chamber of JKI in flowerpots with mainly Euonymus fortunei (Turcz.) Hand.–Mazz. and one with Prunus laurocerasus L. Adults had been collected 5 days previously in a hedgerow with ornamental shrubs in the JKI area.
3 premature larvae: flowerpot with Euonymus fortunei, climate chamber, JKI, 13.12.2012: 2 ex. These specimens were bred to produce pupae and transferred to Hannover for regular pupal control. As there was no further development, they were taken out on 25.01.2013; flowerpot with Prunus laurocerasus, climate chamber of JKI, 14.03.2013: 1 ex.
12 mature larvae: flowerpot with Euonymus fortunei, JKI, climate chamber, 24.08.2012: 1 ex. (the first mature larva after 3 months and 3 weeks of development), 01.11.2012: 2 ex., do., 13.12.2012: 5 ex. (4 of them were used for regular pupae control; as there was no pupation, they were taken out on 25.01.2013), 14.03.2013: 2 ex., flowerpot with Prunus laurocerasus, JKI, climate chamber, 14.03.2013: 2 ex.
Body length: 6.5–7.7 mm, body width at the widest part (level of first abdominal segment): 2.0–2.5 mm, head width: 1.10–1.17 mm, head height: 0.90–1.00 mm.
Body (Figs
Peritelus sphaeroides mature larva, habitus and chaetotaxy. 28 Thoracic segments lateral view 29 First abdominal segment lateral view 30 Abdominal segments 7–10 lateral view 31 Abdominal segments 7–10 ventral view 32 Abdominal segments 7–10 dorsal view Abbreviations: Th. 1–3 – thoracic segments 1–3, Abd. 1–10 – abdominal segments 1–10, setae: as – alar, ps – pleural, eps – epipleural, ds – dorsal, lsts – laterosternal, eus – eusternal, pda – pedal, pds – postdorsal, prns – pronotal, prs – prodorsal, sps – spiracular, sts – sternal, ts – terminal.
Head (Fig.
Peritelus sphaeroides mature larva, body parts. 41 Maxillolabial complex ventral aspect 42 Right maxilla apical part dorsal aspect 43 Right maxilla apical part ventral aspect. Abbreviations: setae: dms – dorsal malar, ligs – ligular, mbs – malar basiventral, mps – maxillary palp, pfs – palpiferal, prms – prelabial, pms – postlabial, stps – stipal, vms – ventral malar.
6 premature larvae: Germany, Niedersachsen, Hannover-Vahrenheide, Kugelfangtrift, nutrient-poor sandy grassland, collected at sparsely grown sites under Plantago lanceolata, 02.10.2011: 3 ex., 11.11.2011: 3 ex.
3 mature larvae: same site as before, 02.10.2011: 1 ex., 11.11.2011: 1 ex.; Denmark, Syddanmark, Emmerlev Klev near Højer, moraine at the sandy sea shore of the North Sea, collected on 13.08.2015: 1 ex., between the roots of Plantago maritima subsp. maritima, very probably; P. lanceolata was also present nearby.
Body length: 6.5–8.2 mm, body width at the widest part (level of first abdominal segment): 2.4–3.4 mm, head width: 0.97–1.03 mm, head height: 0.75–0.83 mm.
Body (Figs
Philopedon plagiatum mature larva, habitus and chaetotaxy. 44 Thoracic segments lateral view 45 First abdominal segment lateral view 46 Abdominal segments 7–10 lateral view 47 Abdominal segments 7–10 ventral view 48 Abdominal segments 7–10 dorsal view. Abbreviations: Th. 1–3 – thoracic segments 1–3, Abd. 1–10 – abdominal segments 1–10, setae: as – alar, ps – pleural, eps – epipleural, ds – dorsal, lsts – laterosternal, eus – eusternal, pda – pedal, pds – postdorsal, prns – pronotal, prs – prodorsal, sps – spiracular, sts – sternal.
Head (Fig.
Philopedon plagiatum mature larva, body parts. 56 Maxillolabial complex ventral aspect 57 Right maxilla apical part dorsal aspect 58 Right maxilla apical part ventral aspect. Abbreviations: setae: dms – dorsal malar, ligs – ligular, mbs – malar basiventral, mps – maxillary palp, pfs – palpiferal, prms – prelabial, pms – postlabial, stps – stipal, vms – ventral malar.
3 mature larvae: Germany, Brandenburg, Cottbus: Kittlitz, collected on 09.08.2011 in a permanent field of Medicago sativa L., together with a Tanymecus pupa and many larvae of Otiorhynchus ligustici (Linnaeus, 1758).
12 first instar larvae: A female collected in the field (Kittlitz) laid eggs on 17.05.2012 in the laboratory in Hannover. Larvae, hatched from eggs on 31.05.2012, were used for this study.
Body length: 8.3–10.0 mm, body width at the widest part (level of first abdominal segment): 2.5–3.2 mm, head width: 1.6–1.8 mm, head height: 1.4–1.6 mm.
Body (Figs
Tanymecus palliatus mature larva, habitus and chaetotaxy. 59 Thoracic segments lateral view 60 First abdominal segment lateral view 61 Abdominal segments 7–10 lateral view 62 Abdominal segments 7–10 ventral view 63 Abdominal segments 7–10 dorsal view. Abbreviations: Th. 1–3 – thoracic segments 1–3, Abd. 1–10 – abdominal segments 1–10, setae: as – alar, ps – pleural, eps – epipleural, ds – dorsal, lsts – laterosternal, eus – eusternal, pda – pedal, pds – postdorsal, prns – pronotal, prs – prodorsal, sps – spiracular, sts – sternal, ts – terminal.
Head (Fig.
Tanymecus palliatus mature larva, body parts. 68 Maxillolabial complex ventral aspect 69 Right maxilla apical part dorsal aspect 70 Right maxilla apical part ventral aspect. Abbreviations: setae: dms – dorsal malar, ligs – ligular, mbs – malar basiventral, mps – maxillary palp, pfs – palpiferal, prms – prelabial, pms – postlabial, stps – stipal, vms – ventral malar.
1 | Abdominal segment 9 about regular structure (type “A” Van |
2 |
– | Abdominal segment 9 strongly reduced, consisting of four well-isolated lobes (type “B” Van |
3 |
2 | Abdominal segment 9 with 3 ds, each pedal area with 6 pda, meso- and metathorax with 3 ss each, Se conical-like | Graptus triguttatus triguttatus |
– | Abdominal segment 9 with 4 ds, each pedal area with 4 pda, meso- and metathorax with 2 ss each, Se cushion-like | Peritelus sphaeroides |
3 | Abdominal segment 9 with 2 ds, each pedal area with 9 pda, meso- and metathorax with 3 ss each, abdominal segments 1–6 with 3–4 eps, clypeus almost as wide as labrum | Philopedon plagiatum |
– | Abdominal segment 9 with 3 ds, each pedal area with 6 pda, meso- and metathorax with 2 ss each, abdominal segments 1–6 with 2 eps, clypeus twice as long as labrum | Tanymecus palliatus |
There are some strange statements about the number of larval instars in the larval stage of species of genus Tanymecus.
In T. dilaticollis Gyllenhal, 1834,
For the exact determination of the number of larval instars we summarized and assessed our own measuring data and added data from literature, if necessary (Tables
Due to the dubiousness of the number of larval instars in T. dilaticollis given by
We also needed to determine the number of larval instars for both species: there are data for L1 and for mature larvae, and in T. dilaticollis there are also measurements for several instars, even if (especially in the higher instars) the data are doubtful.
The determination of larval instars is mainly based on the method of
In Mitoplinthus caliginosus (Fabricius, 1775) (subfamily Molytinae), after comparison of the growth factors 1.35, 1.4 and 1.5, the best approximation was found with a value of 1.4 for head capsule width (
For larval instar determination in Tanymecus we tested four values between 1.4 and 1.5 to achieve the best approximation of larval growth. We started with the L1 larva that we received from egg-laying of adult weevils (head width 0.38 mm) and calculated the subsequent instars with the selected GF values until 1.71 mm, the head width of the mature larvae, was achieved. For this procedure, five steps were needed. Higher GF values, as for example 1.538 in Rhinocyllus conicus, were excluded because of the reduced number of larval instars in this rather distantly related subfamily (Table
From Table
For this approximation it is only necessary to know the head width of the L1 larva and that of the last instar. And the HW of the last instar can be assessed from the HW of the adult weevil, as it is shown in Table
An instar determination is also possible for Graptus triguttatus. According to
In Peritelus sphaeroides and Philopedon plagiatum an instar determination is impossible due to the absence of L1 head width data. It can only be concluded from the data for premature larvae in Philopedon plagiatum (Table
Head width measuring data of the species studied. Results in mm; n – number of specimens measured, in adults behind eyes; L1 – first instar larva; ML – mature larva; *: an assignment to this instar is doubtful. A transfer to ‘mature larvae’ would change the average value only slightly; **: data from
Species | Larval instars | Pupa | Adult | ||
---|---|---|---|---|---|
L1 larvae | Premature larvae | Mature larvae | |||
Graptus triguttatus (L1 data from |
0.331; 0,341; 0.351 | 0.572; 0.603; 1.001; 1.072; 1.171 | 1.373; 1.402; 1.431; 1.472; 1.506; 1.534; 1.551 | 1.131; 1.171; 1.201 | 1.051; 1.151; 1.21; 1.253; 1.33; 1.41; 1.451; 1.51 |
Mean value (x¯) | 0.34 | 0.806 | 1.471 | 1.167 | 1.283 |
Peritelus sphaeroides | – | – | 0.871; 0.902; 1.105; 1.133; 1.174 | 1.001; 1.051 | 0.751; 0.851; 0.952; 1.02; 1.0251; 1.21 |
Mean value (x¯) | – | – | 1.083 | 1.025 | 1.025 |
Philopedon plagiatum | – | 0.601; 0.674; 0.731 | 0.971;1.001; 1.031 | – | 1.051; 1.12; 1.2251; 1.251; 1.3751; 1.42 |
Mean value (x¯) | – | 0.668 | 1.00 | – | 0.966 |
Philopedon plagiatum (from |
– | 0.572; 0.611; 0.631; 0.641; 0.681; 0.721; 0.751*; 0.791* | 0.861; 0.892; 0.962; 1.001; 1.072; 1.081; 1.12; 1.141; 1.171; 1.181; 1.212 | – | – |
Mean value (x¯) | – | 0.662 | 1.056 | – | – |
Tanymecus dilaticollis | – | – | – | – | 1.302 |
Mean value (x¯) | – | – | – | – | 1.30 |
Tanymecus palliatus | 0.341; 0.372; 0.385; 0.392; 0.402 | – | 1.621; 1.701; 1.801 | – | 1.2751;1.4251; 1.51; 1.551; 1.61 |
Mean value (x¯) | 0.380 | – | 1.717 | – | 1.470 |
Head width measuring data of Tanymecus larvae and adults for larval instar determination. *: collected together with T. palliatus pupae; **: calculated from the ratio of adult’s HW to HW of the mature larva.
Instar | Mean value (mm) | Specimens measured | Source |
---|---|---|---|
Tanymecus dilaticollis | |||
L1 | 0.35 | 12 larvae |
|
L2 | 0.56 | 7 larvae |
|
L3 | 0.77 | 5 larvae |
|
L4 | 1.27 | 4 larvae |
|
Mature larva | 1.51** | – | calculated value |
Adult weevil | 1.30 | 2 adults | own data |
Tanymecus palliatus | |||
L1 | 0.38 | 12 larvae | own data (see Tab. |
Mature larva* | 1.71 | 3 larvae | own data (see Tab. |
Mature larva | 1.71 | 5 larvae |
|
Adult weevil | 1.47 | 5 adults | own data |
Larval instar determination for Tanymecus dilaticollis and T. palliatus. All measuring data in mm; initial data bold, calculated data in italics; target data of the approximation bold and in italics.
Tanymecus palliatus | |||
Growth factor (to be tested) | 1.40 | 1.45/1.46 | 1.50 |
L1 (measured) | 0.38 | 0.38 | 0.38 |
L2 (calculated) | 0.532 | 0.551/0.555 | 0.57 |
L3 (calculated) | 0.745 | 0.799/0.810 | 0.855 |
L4 (calculated) | 1.042 | 1.158/1.183 | 1.283 |
L5 (calculated) | 1.460 | 1.680/1.727 | 1.924 |
Mature larva (measured) | 1.71 | 1.71 | 1.71 |
Tanymecus dilaticollis* | |||
Growth factor (to be tested) | 1.40 | 1.44/1.45/1.46 | 1.50 |
L1 (measured) | 0.35 | 0.35 | 0.35 |
L2 (measured) | 0.56 | 0.56 | 0.56 |
L2 (calculated) | 0.49 | 0.504/0.508/0.511 | 0.525 |
L3 (measured) | 0.77 | 0.77 | 0.77 |
L3 (calculated) | 0.686 | 0.726/0.736/0.746 | 0.7875 |
L4 (measured) | 1.27 | 1.27 | 1.27 |
L4 (calculated) | 0.960 | 1.045/1.067/1.089 | 1.181 |
L5 (calculated) | 1.345 | 1.505/1.55/1.590 | 1.772 |
Mature larva (calculated; see Tab. |
1.51 | 1.51 | 1.51 |
Number of setae in mature larvae of Graptus triguttatus triguttatus (a), Peritelus sphaeroides (b), Philopedon plagiatum (c), Tanymecus palliatus (d).
Part of body | Setae | a | b | c | d |
---|---|---|---|---|---|
Prothorax | prns | 8 | 9 | 9 | 11 |
ps | 2 | 2 | 2 | 2 | |
eus | 1 | 1 | 1 | 1 | |
pda | 6 | 4 | 9 | 6 | |
Meso-, metathorax | prs | 1 | 1 | 1 | 1 |
pds | 4 | 4 | 4 | 4 | |
as | 1 | 1 | 2 | 2 | |
ss | 3 | 2 | 3 | 2 | |
eps | 1 | 1 | 1 | 1 | |
ps | 1 | 1 | 1 | 1 | |
eus | 1 | 1 | 1 | 1 | |
pda | 6 | 4 | 9 | 6 | |
Abdominal segments 1–8 | prs 1-7 | 1 | 1 | 1 | 1 |
prs 8 | 1 | 1 | 1 | 1 | |
pds 1-7 | 5 | 5 | 5 | 5* | |
pds 8 | 4 | 4 | 5 | 4 | |
ss 1-7 | 2 | 2 | 2 | 1 | |
ss 8 | 1 | 1 | 1 | 0 | |
eps 1-7 | 2 | 2 | 3-4 | 2 | |
eps 8 | 2 | 2 | 2 | 2 | |
ps 1-7 | 2 | 2 | 2 | 2 | |
ps 8 | 2 | 2 | 2 | 2 | |
lsts 1-7 | 1 | 1 | 1 | 1 | |
lsts 8 | 1 | 1 | 1 | 1 | |
eus 1-7 | 2 | 2 | 2 | 2 | |
eus 8 | 2 | 2 | 2 | 2 | |
Abdominal segment 9 | ds | 3 | 4 | 2 | 3 |
ps | 2 | 1 | 1 | 1 | |
sts | 2 | 2 | 2 | 2 | |
Abdominal segment 10 | ts | 2 | 2 | 0 | 3 |
Head | des | 4 | 4 | 5 | 4 |
fs | 2 | 2 | 3 | 2 | |
ls | 2 | 2 | 2 | 2 | |
pes | 3 | 7 | 4 | 5 | |
ves | 1 | 0 | 1 | 2 | |
Mouthpart | cls | 2 | 2 | 2 | 2 |
lrs | 3 | 3 | 3 | 3 | |
mes | 2 | 2 | 2 | 2 | |
als | 3 | 3 | 3 | 4 | |
ams | 3 | 3 | 3 | 3 | |
mds | 2 | 1 | 2 | 2 | |
des | 7 | 7 | 8 | 7 | |
ves | 4 | 4 | 4 | 4 | |
pfs | 2 | 2 | 2 | 2 | |
stps | 1 | 1 | 1 | 1 | |
pbs | 1 | 1 | 1 | 1 | |
mps | 1 | 1 | 1 | 1 | |
pms | 3 | 3 | 3 | 3 | |
prms | 1 | 1 | 1 | 1 | |
ligs | 3 | 2 | 2 | 2 |
Philopedon belongs to genera with abdominal type ‘B’ larvae together with Strophosoma and Tanymecus (Van
According to
The mature larva of Philopedon plagiatum is described by
In Graptus triguttatus the shape of the body, number and proportion of setae on L1 (according to
From morphological data, the taxonomic position of Byrsopagini is apparently not so clear, but its placement within Entiminae is more plausible than in Molytinae; this is in line with the results of the cladistic and phylogenetic analyses performed by
Moreover, a detailed analysis of the structure of antennae disclosed only ostensible similarities between Graptus and Molytinae, e.g. Mitoplinthus caliginosus described by
Graptus triguttatus triguttatus, mature larva. 71 Head 72 Antenna Mitoplinthus caliginosus mature larva 73 Head 74 Antenna, Philopedon plagiatum mature larva 75 Head 76 Antenna. Abbreviations: as – antennal segment, Se – sensorium, sa – sensillum ampullaceum, sb – sensillum basiconicum, sc – sensillum chaeticum.
Van
The description of the pupa of Graptus triguttatus did not provide any relevant features that could confirm a replacement of this genus in the Entiminae. It seems to be especially difficult to distinguish them from other subfamilies due to the large diversity of both, shape and chaetotaxy, and the absence of features strictly characteristic of Entiminae pupae (
Graptus triguttatus. In January and April 2013 many larvae of different instars were found, and most of them were already mature (Figs
Peritelus sphaeroides. According to
Philopedon plagiatum. A description of the pupa is not available, even though the species attracted attention occasionally in beet fields, horticultural crops and pine plantations in sandy areas (Figs
Habitats, host plants, digging sites and larvae of soil-dwelling weevils. 79 Habitat of Philopedon plagiatum in Southwestern Denmark, sandy sea shore of the North Sea 80 Habitat of P. plagiatum in Hannover-Vahrenheide, Kugelfangtrift, sand path with Plantago lanceolata 81 Digging site of P. plagiatum, sandy soil with P. lanceolataat the Kugelfangtrift 82 Larva of the penultimate instar of P. plagiatum, found in the field in October, just molted, with light head capsule 83, 84 Larva of Peritelus sphaeroides, bred in flowerpots with Euonymus fortunei in a climate chamber 85, 86 Host plant (Plantago lanceolata) and digging site of Graptus triguttatus triguttatus under P. lanceolata in an occasionally mown meadow of the JKI area in Braunschweig 87 Mature or penultimate instar larva of Graptus triguttatus triguttatus, found in the field in Braunschweig in April.
We thank Dr Martin Hommes for the chance to use a climate chamber in the Julius-Kühn-Institute (JKI) in Braunschweig, and Manfred Lehmann and Dr Jörn Lehmhus (JKI) for the presentation of the sites of Tanymecus palliatus and Graptus triguttatus, where these species occurred in high numbers. Vitaliy Nazarenko provided some pages from a hard to obtain source with an old description of larva of T. palliatus.