Research Article |
Corresponding author: Maria Claudene Barros ( mbdene@yahoo.com.br ) Academic editor: DeeAnn Reeder
© 2018 Amanda Cristiny da Silva Lima, Fabio Henrique Souza Cardoso, Samira Brito Mendes, Elmary Costa Fraga, Maria Claudene Barros.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Lima ACS, Cardoso FHS, Mendes SB, Fraga EC, Barros MC (2018) New records of Niceforo’s big-eared bat, Trinycteris nicefori (Sanborn, 1949) (Chiroptera, Phyllostomidae), from the state of Maranhão, Brazil. ZooKeys 787: 127-134. https://doi.org/10.3897/zookeys.787.26538
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Niceforo’s big-eared bat, Trinycteris nicefori (Sanborn, 1949), is a monotypic species which has been recorded in a number of Brazilian states, but has a disjunct distribution in this country. This study presents the first record of T. nicefori in the Brazilian state of Maranhão. The specimens were collected in the municipalities of Godofredo Viana and Cândido Mendes, in fragments of the Amazon forest. One male (forearm: 38.00 mm, weight: 6 g) and one female (39.68 mm, 8 g) specimens were collected. The specimens presented chestnut-colored fur, and a chin with a pair of dermal pads arranged in a V-shape, without a central papilla. The COI gene sequences were plotted in the BOLD Systems platform, which confirmed the morphological identification of the species, with a 99.1% similarity in the male, and 99.4% in the female to existing sequences. This record extends the known distribution of T. nicefori in Brazil by approximately 310 km to the most eastern part of the Amazon Biome.
Bats, COI gene, Maranhão, mitochondrial, range extension
The genus Trinycteris Sanborn, 1949 was originally described as a subgenus of Micronycteris Gray, 1866, and subsequently recognized as a monotypic genus by
Niceforo’s big-eared bat, Trinycteris nicefori, is an insectivore (
The known geographic distribution of T. nicefori ranges from southern Mexico to Central America, Trinidad, Colombia, Venezuela, Guiana, Suriname, Peru, Ecuador, Bolivia, and Brazil (
More than 100 bat species are known to occur in the Amazon region. In the Amazonian domain of the Brazilian state of Maranhão, 47 species have been recorded representing 29 genera (
The specimens of T. nicefori were collected from fragments of forest in the Amazonian domain of Maranhão in April 2017, in the municipalities of Godofredo Viana and Cândido Mendes. The bats were captured in mist-nets, 3 m high, and 9–12 m in length, with a 25 mm mesh, which were fixed to the ground with poles and cords. The age of the specimens was estimated based on the ossification of the phalangeal epiphyses, and the reproductive condition was determined by palpation of the teats and abdomen in the female and the position of the testicles in the male (
The morphological and craniometric measurements were obtained using a manual caliper, following
For the molecular analyses, total DNA was extracted from the samples of muscle tissue using the Wizard Genomic DNA Purification kit (Promega), according to the manufacturer’s instructions. The mitochondrial Cytochrome Oxidase Subunit I (COI) gene was amplified by Polymerase Chain Reaction (PCR) using the primers LCO-1490 and HCO-2198 described by
Two specimens of T. nicefori were captured at the study sites in the Amazonian domain of Maranhão (Table
The analysis of the COI molecular marker on the Bold System platform confirmed the morphological identification of the specimens collected in the present work. These specimens present 99% of similarity with the sequences of T. nicefori of Costa Rica and a divergence of 1–3% in comparison with sequences of T. nicefori from Guiana.
Geographical location of specimens of Trinycteris nicefori collected in the state of Maranhão, with voucher and GenBank accession numbers.
Craniometric measurements, in mm, of Trinycteris nicefori obtained in the present study, compared with the holotype (
Cranial feature | Present study |
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Male | Female | Holotype | Male (n = 3) | Female (n = 2) | |
Greatest length of skull | 20.0 | 21.0 | 20.5 | 19.54–20.39 | 19.71–20.49 |
Condylobasal length | 18.0 | 18.5 | 18.5 | 18.06–18.72 | 17.99–19.07 |
Mastoid breadth | 8.6 | 9.0 | 8.9 | 8.74–9.05 | 8.43–8.62 |
Zygomatic breadth | 8.5 | 9.0 | 9.6 | 8.84–9.51 | 9.05–9.14 |
Breadth of braincase | 7.0 | 7.5 | 8.2 | 7.92–8.22 | 7.97–8.26 |
Postorbital constriction | 4 | 4 | 4.3 | 3.92–4.11 | 4.21 |
Palatal length | 8 | 8 | 8.2 | – | – |
Length of upper tooth row | 7 | 7 | 7.3 | 7.14–7.45 | 6.99–7.56 |
Length of lower tooth row | 7 | 7 | 7.6 | 7.14–7.45 | 6.99–7.56 |
Length of mandible | 13 | 13 | – | – | – |
Given the similarities with species of the genus Carollia Gray, 1838, the coloration of the pelage is not a diagnostic feature of the genus Trinycteris, although the two genera can be distinguished primarily by the absence of the central protuberances of the papilla in Trinycteris (
The body mass and craniometric parameters recorded in the present study were consistent with those reported by
The available data on T. nicefori show an important gap in the Amazon biome to the east of Brazil, indicating a disjunctive distribution between the Amazon and Cerrado biomes. The species is also absent from the Caatinga and Cerrado (sensu stricto) biomes, which indicates that it probably prefers mesic environments and is relatively intolerant to arid conditions (
The present study registered the occurrence of T. nicefori in the Brazilian state of Maranhão, based on the analysis of morphological and molecular data. This is the first record of the species for the state, where it was found in the Amazonian domain. This record extends the known distribution of T. nicefori 303 km to Godofredo Viana and 310 km to Cândido Mendes.
Foundation for Research and Scientific and Technological Development of Maranhão (FAPEMA) and National Council for Scientific and Technological Development (CNPq).