Research Article |
Corresponding author: Benoit Vincent ( amastus@gmail.com ) Academic editor: Christian Schmidt
© 2018 Benoit Vincent.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Vincent B (2018) Two new species of the Neotropical Lophocampa hyalinipuncta (Rothschild) group (Lepidoptera, Erebidae, Arctiinae). In: Schmidt BC, Lafontaine JD (Eds) Contributions to the systematics of New World macro-moths VII. ZooKeys 788: 57-67. https://doi.org/10.3897/zookeys.788.26325
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Two new species of Lophocampa Harris are described and illustrated, Lophocampa azuayensis sp. n., and Lophocampa carpishensis sp. n. Both new species were confused with Lophocampa hyalinipuncta (Rothschild, 1909), and a comparative diagnosis is provided.
Deux nouvelles espèces de Lophocampa Harris sont décrites et illustrées, Lophocampa azuayensis sp. n., et Lophocampa carpishensis sp. n. Une comparaison avec Lophocampa hyalinipuncta (Rothschild, 1909), l’espèce avec laquelle ces taxa étaient confondus, est proposée.
Arctiinae , Bolivia, Ecuador, Erebidae , Lepidoptera , Lophocampa , new species, Peru
The genus Lophocampa Harris, 1841 is one of the most speciose in the Neotropical tiger-moths, with 80 species and eight subspecies (
During the consultation of Neotropical Arctiini specimens in the Thomas Witt collection, housed at the
This group of species, with forewings brown and bands formed by large white spots, is very characteristic. It cannot be confused with other species of the Andean cordillera, except for the species of the group Lophocampa atriceps, whose white spots are much smaller. A discrimination of Lophocampa hyalinipuncta (also valid for the species described in this article) with the group Lophocampa atriceps is published in
Abdomens were softened in warm 20% KOH for 15 minutes. Scales were removed with a brush. Abdominal sclerites and genitalia were stained with Chlorazol Black E dissolved in distilled water, then dehydrated, positioned and mounted on permanent slides in Euparal. Photographs from slides mounts were made using a Jenoptik ProgRes C10 camera attached to a Leica MZ 16 stereomicroscope. Dissections were photographed with a Nikon CoolPix 4500 Camera attached to a Nikon SMZ-10A stereomicroscope.
Repository abbreviations are as follows:
PUCE Pontifica Universidad Católica del Ecuador, Quito, Ecuador
BVC Personal collection of Benoit Vincent, Quint-Fonsegrives, France
MLC Personal collection of Michel Laguerre, Léognan, France
MWM Museum Witt, München, Germany
Tissue samples of the new species described in the present work were processed through DNA barcoding at the Canadian Centre for DNA Barcoding in Guelph (Ontario, Canada), along with many other arctiid species as the target of a DNA barcoding project for neotropical tiger moths, developed within the iBOL Lepidoptera campaign (see www.lepbarcoding.org for further details). DNA extraction, PCR amplification, and sequencing followed the protocols already described in
The taxa used in this study are detailed in Table
DNA sequence divergence between holotypes of the new species and specimens of L. hyalinipuncta, based on the barcode fragment of the COI gene. The percent divergence from averaging over all sequence pairs is based on analyses using the Kimura 2-parameter model. The analysis involved the six specimens shown in Figure
L. azuayensis sp. n. | L. carpishensis sp. n. | L. hyalinipuncta | |
---|---|---|---|
L. azuayensis sp. n. | – | ||
L. carpishensis sp. n. | 2.1 | – | |
L. hyalinipuncta | 2.3 | 1.6 | – |
Halisidota (sic) hyalinipuncta Rothschild, 1909: 217.
4 male syntypes. Type locality: Agualani, Carabaya, [Puno], Peru, 9000 feet [2740m], (wet season), Dec[ember] 1905 (G.R. Ockenden). One specimen from the type locality is labelled “TYPE” and “Lectotype male Lophocampa hyalinipuncta Rothshild designated by Vincent, 2018” in
Peru (Puno) and Bolivia (La Paz, Cochabamba, and Chuquisaca) (Figure
The description of the habitus of Lophocampa hyalinipuncta made by
The male genitalia are identical to that of L. carpishensis sp. n. with the following differences: Uncus narrow; cucullus shorter, transtilla with the end of the triangular tongue more acute. Vesica with dorsal diverticula carrying smaller cornuti with very small spines; two lateral larger diverticuli with wider spines.
Holotype – ♂, Ecuador, Azuay province, 5 km road LA PAZ – ONA, 3°21'50"S; 79°11'31"W, 06.02.2012; 3020 m, leg. R. Brechlin & V. Siniaev, genitalia dissected by Michel Laguerre. n° ML 2514, Barcode ID GWOTP625-15, Sample ID BC
Paratype, ♀, same data as holotype, genitalia dissected by B. Vincent. n° BV 482, Barcode ID GWOTP626-15, Sample ID BC
See Table
Female identical to male except as noted. Head. Antenna bipectinate, female with pectinations shorter than male, brown with yellowish base and brownish cilia. Frons brown on the inferior half, white on the superior half. Vertex brown, with white setae near the antenna insertion. Palpi erected, brown, the third article very short with white setae at the apex.
Thorax. Patagia white with a square brown spot centred with whitish. Tegulae white except base and inner edge dark brown; presence of two brown spot centred with whitish. Thorax light yellow with a strong medial black line. Legs, femur brown on basal half, bright yellow on apical half, except for brown apex. Prothoracic tibia and tarsi brown on the outer side, whitish on the inner side. Meso- and metathoracic tibia and tarsi brown, ringed with white on the outer side, whitish on the inner side. Forewing. Forewing length 22 mm (male) and 25 mm (female). Brown, very lightly sprinkled with light brown. The venation slightly darker than the ground colour. A series of bands formed by white spots as follows: a basal band consisting of three white spots ; an post-basal band broken at medial vein an antemedial band incomplete, without spots on costa and anal border; a medial line incomplete, limited to a spot on the costa and a large spot that reaches the medial vein an oblique postmedial band, complete, the spot between veins M2 and M3 very small; a complete subterminal line, made of well aligned rounded spots whose edges almost reach the margin ; a terminal line of white dots. Hindwing. White, slightly tinged with brown markings at the apex.
Abdomen: Tergites pale yellow with long brownish setae in the basal half of the medial axis, with a lateral series of brown spots. Sternites whitish with brown patches, these centred with yellowish. Male genitalia. Uncus rectangular and setose enlarged in the medial area. Tegumen short. Saccus tongue shaped, weakly sclerotized and folded ventrally. Valvae symmetrical, wide at the base then narrowed sharply into a pointed apical end slightly inverted ventrally, greatly exceeding the uncus apex. Cucullus slender, elongate, with an apex slightly curved ventrally. Juxta narrow with two arms fused apically. Transtilla formed by two slightly diverging triangular tongues, separated by a central unsclerotised area. Aedeagus: Penis straight, short, caecum penis present. Vesica wide with four diverticuli: one dorsal, the largest, with a patch of long spines; two lateral, simple, with at the apex a very dense patch of short spines; the last, ventral, multi-lobed with a patch of sparse short spines.
Female genitalia. Apophyses posteriores straight. Apophyses anteriores shorter, very slightly curved. Papillae anales rectangular and setose. Pseudopapillae small. Dorsal saccular pheromone glands reduced. Ductus bursae short, rectangular with an extension on the right (ventral view). Corpus bursae very reduced, wrinkled, formed by two rounded lobes folded one over the other.
Genitalia male and female of Lophocampa species. 5–6 Lophocampa azuayensis sp. n genitalia (5), penis (6), holotype male 7–8 Lophocampa carpishensis sp. n., genitalia (7), penis (8), holotype male; Lophocampa azuayensis sp. n genitalia female (9); Lophocampa carpishensis sp. n., genitalia female (10).
The specific epithet, azuayensis, refers to the province of Azuay, Ecuador where the type locality is located.
Ecuador, Azuay province (Figure
Unknown.
Holotype – ♂, Peru, Huanuco, [Paso] Carpish, 2000–2800 m, IV-2009, via R. Marx, genitalia dissected by B Vincent n° BV 484 [
1 ♂ and 2 ♀, same data as holotype, genitalia dissected by B. Vincent respectively n° BV 427, BV 428 and BV 485, in BVC. 1 ♂, Peru, Carpish, Hua[nuco], 21.8.68, ex coll J. Dubois, in
See Table
Head. Antenna bipectinate, yellowish on basal half, brown on apical half with brownish cilia. Frons and vertex brown, mixed with white hairs. Palpi erected, black, the third article very short with white hair on the ventral side at the apex.
Thorax. Patagia white with a square brown spot centered with whitish. Tegulae white except for inner edge and center light dark brown. Thorax light yellow. Legs: Femur bright yellow, except brown apex. Tibia and tarsi of the first pair, brown on the outer surface, whitish on the inner side. Tibia and tarsi of the second and third pairs, brown ringed with white. Forewing. Forewing length 23 mm (male) and 25 mm (female). Brown, slightly lighter between the subterminal band and the fringe. Presence of a yellow spot at the base of the wing and a series of bands formed by white spots and organized as follows: a basal band consisting of three white spots; a postbasal band broken at median vein; an ante median band complete, wide at the anal edge and reducing to the costa; a median band incomplete which merges, between CuA1 and M3 with a postmedian band oblique, complete. Complete subterminal band, formed of small and compressed spots; a terminal line of white dots on the margin, barely visible. Except for the basal and subterminal band, spots hyaline white, excluding costa and anal border ivory white. Spots on the subterminal band ivory white. Hindwing. White slightly tinged with light brown marks on apex. Ventrally, costa ivory white with several brown spots.
Abdomen: Tergites yellow with long brownish hair in the basal half. The posterior edges of the tergites are highlighted in black. Sternites whitish with brown patches centered with yellowish.
Male Genitalia. Identical to that of L. azuayensis with the following differences: Uncus rectangular, slightly narrower in the apical half and not widened in the median area. Valvae, broad at the base, which gradually narrows to a very pointed apical end. Cuculus with an apex strongly curved ventrally. Vesica wide with three diverticuli: one dorsal, the largest, with a patch of long spines; one lateral, simple, without spines; the last, ventral, simple, with a patch of cornuti with long spines and a second patch with shorter spines.
Female identical to male except for the following differences: antennae with pectinations shorter than male. Wingspan slightly larger. Median and postmedian band incomplete, interrupted between CuA2 and the anal edge. The spot of the post median band between CuA1 and CuA2 is kidney-shaped.
Genitalia. Apophyses posteriores straight strongly narrowed near the base. Apophyses anteriores as long, curved. Papillae anales rectangular and setose. Pseudopapillae small. Dorsal saccular pheromone glands reduced Ductus bursae short, rectangular with an extension on the right (ventral view). Corpus bursae very reduced, wrinkled, formed by two rounded lobes folded one over the other.
The name carpishensis refers to the type locality: Paso Carpish (Carpish Pass), whose most famous place is the tunnel Carpish which is a 2707 m high and marks the separation between the vegetation of mattoral dry Pacific side and forest vegetation Amazon cloud side. The humid montane forests of Carpish are important for their high diversity and endemic species.
Peru (Amazonas, Huanuco, and Pasco).
Unknown.
COI sequence of specimens identified formerly as L. hyalinipuncta segregates into three clades (Figure
Lophocampa azuayensis sp. n. is only known from the type locality, from a high elevation area of Ecuador that is rarely explored. It would be interesting to determine if the species is present at lower altitudes, or if it is found only above 3000 m. Furthermore, it would be interesting to clarify if the species occupies the western or eastern slopes of the Andes, knowing that
Lophocampa carpishensis sp. n. has a larger known distribution with, in addition to the typical locality, localities to the north or south at altitudes always between 2000 and 3000 m. Finally, L. hyalinipuncta has a more southern distribution, at the same altitudes as L. carpishensis sp. n (Figure
Morphological differences between the taxa are not obvious, but the pattern of the forewings, in addition to characters of the male and female genitalia, is sufficiently different to easily identify the three species (Table
Comparison of diagnostic differences among L. azuayensis sp. n., L. carpishensis sp. n. and L. hyalinipuncta.
Character | L. azuayensis sp. n. | L. carpishensis sp. n. | L. hyalinipuncta. |
Base of the forewing | Without a yellow spot | With a yellow spot | Without a yellow spot |
Post median band of the forewing | With a spot between M2–M3 smaller than spot between M1–M2 | With a spot between M2–M3 uniform than spot between M1–M2 | With a spot between M2–M3 identical to spot between M1–M2 |
Subterminal band of the forewing | With rounded spots whose edges reach almost the margin and form a regular alignment | With very small and compressed spots not aligned with the margin | With rounded spots not aligned with the margin |
Male with uncus | Enlarged in the median area | Narrowed in the apical half | Longer and slightly narrowed in the apical half |
Male with valvae | Narrowed sharply to an apex acute | Narrowed gradually to an apex acute | Narrowed sharply to an apex bevelled |
Male with vesica | consisting of 4 diverticuli 2 lateral with spines | consisting of 3 diverticles, 1 lateral without spines | consisting of 4 diverticles, 2 lateral with spines |
Female with apophyses posteriores | Not strongly narrowed near their base | strongly narrowed near their base | Not strongly narrowed near their base |
The author would like to acknowledge Tomas Witt that allowed the access to and borrowing of Arctiini specimens from his collection, and Axel Hausmann for his receiving at