Research Article |
Corresponding author: Nickolas G. Kavallieratos ( nick_kaval@aua.gr ) Academic editor: Kees van Achterberg
© 2018 Eleftherios Alissandrakis, Panagiota Psirofonia, Nickolas G. Kavallieratos, Saša S. Stanković, Vladimir Žikić.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Alissandrakis E, Psirofonia P, Kavallieratos NG, Stanković SS, Žikić V (2018) Parasitic wasps related to Prays oleae (Bernard, 1788) (Lepidoptera, Praydidae) in olive orchards in Greece. ZooKeys 773: 143-154. https://doi.org/10.3897/zookeys.773.25402
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The olive moth, Prays oleae (Bernard, 1788) (Lepidoptera: Praydidae) is categorised among the most devastating insect pests of olives, whose anthophagous and carpophagous generations can cause yield loss up to 581 and 846 kg of fruit per ha, respectively. In this study, results of the captured parasitoids in olive tree (Olea europaea Linnaeus, 1753) orchards, or infested olive plant material in Crete, Greece, is presented. Five of the six identified species captured in trap devices are related to P. oleae, i.e., Chelonus elaeaphilus Silvestri, 1908, Chelonus pellucens (Nees, 1816), Apanteles xanthostigma (Haliday, 1834), Diadegma armillatum (Gravenhorst, 1829), and Exochus lentipes Gravenhorst, 1829. The species Eupelmus urozonus Dalman, 1820 and Pnigalio mediterraneus Ferrière & Delucchi, 1957 were reared from infested P. oleae leaves. Chelonus pellucens is reported for the first time from Greece. According to the international literature, 59 hymenopterous and dipterous parasitoid species are associated with P. oleae in Europe.
Diptera , Greece, Hymenoptera , parasitoids, Prays oleae
Olive trees growing has been traditionally localised in the Mediterranean Basin for thousands of years, where almost 97.9% of the cultivated areas are located (
Prays oleae is one of the main pests infesting olives of commercial production, since larvae of the first, second, and third generations attack flowers, fruits, and leaves, respectively (
Previous research has revealed a wide parasitoid spectrum that is related to P. oleae, resulting to biological control efforts against this pest. The first parasitoid used in biocontrol program was Trichogramma embryophagum (Hartig, 1838) (Hymenoptera: Trichogrammatidae) in former Yugoslavia (
Although there are previous records concerning the occurrence of P. oleae parasitoids in Greece, there are no data available from the island of Crete, the most important olive production area with almost 200,000 ha cultivated with olive trees (i.e., nearly 25% of the total island area is covered with olive plantations;
All parasitoids were collected in olive orchards from the island of Crete, Greece from June to October 2017. A part of the material was captured in five glass McPhail trap devices, installed from June to October in an olive orchard at Messara (Crete) that covers an area of approx. 0.5 ha baited with 200 ml aqueous solution of 2% hydrolysed protein (Entomela 75 SL, 25% w/w urea; BASF Hellas, Amaroussion, Greece). Each trap device was placed with its lower part at a height of 2 m from the ground. The distances among trap devices were approx. 100 m. The solution was replaced every week. Additional specimens were reared from P. oleae infested plant material (O. europaea var. koroneiki). Infested leaves by P. oleae larvae were collected from olive trees, separately transferred into plastic vials covered with mesh, and transferred to the laboratory. Vials were maintained at 25 °C and 60% relative humidity and inspected daily for emergence of parasitoids. All parasitoid individuals, either from trap devices or plant material, were preserved in 96% alcohol. Specimens were dissected and slide mounted in Berlese medium. The identification of the captured and reared specimens was conducted under a Nikon SM2 745T binocular stereomicroscope (Nikon CEE GmbH, Wien, Austria) or an Olympus SZX9 (Olympus Corporation, Tokyo, Japan) using appropriate keys (
Additional to field research, we critically reviewed all recorded parasitoids of P. oleae in Greece and Europe indicating the pest’s stage they attack. The synonymy among taxa was checked and adopted according to online databases (
In total, five out of six species captured in McPhail trap devices are related to P. oleae, i.e., C. elaeaphilus, Chelonus pellucens (Nees, 1816) (Hymenoptera: Braconidae), Apanteles xanthostigma (Haliday, 1834) (Hymenoptera: Braconidae), Diadegma armillatum (Gravenhorst, 1829) (Hymenoptera: Ichneumonidae), and Exochus lentipes Gravenhorst, 1829 (Hymenoptera: Ichneumonidae), while two species were reared from P. oleae infested olive leaves.
The exhaustive investigation of the international literature revealed 59 hymenopterous and dipterous parasitoid species that attack P. oleae in Europe; 14 Braconidae, 2 Chalcididae, 1 Encyrtidae, 20 Eulophidae, 1 Eupelmidae, 7 Icheumonidae, 1 Platygastridae, 3 Pteromalidae, 2 Tachinidae, and 8 Trichogrammatidae (Table
Parasitoids of Prays oleae recorded in Europe and their presence in Greece: (+) recorded, (-) not recorded.
Family | Species | Source of host record | Host stage attacked | Recorded or not in Greece |
---|---|---|---|---|
Braconidae | Aleiodes circumscriptus (Nees, 1834) |
|
larva | + |
Aleiodes gastritor (Thunberg, 1822) |
|
larva | + | |
Apanteles xanthostigma (Haliday, 1834) |
|
larva | + | |
Bracon hebetor Say, 1836 |
|
larva | + | |
Bracon laetus (Wesmael, 1838) |
|
larva | + | |
Bracon crassicornis Thomson, 1892 |
|
larva | + | |
Chelonus (Microchelonus) elaeaphilus Silvestri, 1908 |
|
larva | + | |
Chelonus (Microchelonus) silvestrii (Papp, 1999) |
|
larva | - | |
Chelonus (Parachelonus) pellucens (Nees, 1816) |
|
larva | - | |
Clinocentrus testaceus (Kriechbaumer, 1894) |
|
larva | - | |
Dolichogenidea dilecta (Haliday, 1834) |
|
larva | - | |
Dolichogenidea ultor (Reinhard, 1880) |
|
larva | - | |
Meteorus rubens (Nees, 1811) |
|
larva | + | |
Phanerotoma dentata (Panzer, 1805) |
|
larva | + | |
Chalcididae | Hockeria bifasciata Walker, 1834 |
|
larva | - |
Hockeria unicolor Walker, 1834 |
|
larva | + | |
Encyrtidae |
Ageniaspis fuscicollis (Dalman, 1820) var. praysincola Silvestri, 1907 |
|
larva | + |
Eulophidae | Asecodes erxias (Walker, 1848) |
|
larva | + |
Baryscapus nigroviolaceus (Nees, 1834) |
|
larva | - | |
Chrysocharis gemma (Walker, 1839) |
|
larva | + | |
Chrysocharis nephereus (Walker, 1839) |
|
larva | + | |
Cirrospilus elongatus Boucek, 1959 |
|
larva | - | |
Dicladocerus westwoodii Westwood, 1832 |
|
larva | + | |
Elasmus arcuatus Ferrière, 1947 |
|
larva | - | |
Elasmus flabellatus (Fonscolombe, 1832) |
|
larva | + | |
Elasmus masii Ferrière, 1929 |
|
larva | - | |
Elasmus nudus (Nees, 1834) |
|
larva | - | |
Elasmus steffani Viggiani, 1967 |
|
larva | + | |
Elasmus westwoodi Giraud, 1856 |
|
larva | + | |
Euderus albitarsis (Zetterstedt, 1838) |
|
larva | - | |
Hemiptarsenus unguicellus (Zetterstedt, 1838) |
|
larva | - | |
Pediobius bruchicida (Rondani, 1872) |
|
larva/ pupa | + | |
Pnigalio agraules (Walker, 1839) |
|
larva/ pupa | + | |
Pnigalio epilobii Bouchek, 1966 |
|
larva/ pupa | + | |
Pnigalio longulus (Zetterstedt, 1838) |
|
larva/ pupa | + | |
Pnigalio mediterraneus Ferrière & Delucchi, 1957 |
|
larva/ pupa | + | |
Pnigalio pectinicornis (Linnaeus, 1758) |
|
larva/ pupa | + | |
Eupelmidae | Eupelmus urozonus Dalman, 1820 |
|
larva | + |
Ichneumonidae | Diadegma armillatum (Gravenhorst, 1829) |
|
larva/ pupa | + |
Diadegma semiclausum (Hellén, 1949) |
|
larva/ pupa | + | |
Exochus lentipes Gravenhorst, 1829 |
|
larva | - | |
Ichneumonidae | Himertosoma superbum Schmiedeknecht, 1900 |
|
larva/ pupa | - |
Itoplectis alternans (Gravenhorst, 1829) |
|
larva/ pupa | - | |
Lissonota superbator Aubert, 1967 |
|
larva | + | |
Scambus elegans (Woldstedt, 1877) |
|
larva | - | |
Platygastridae | Platygaster apicalis Thomson, 1859 |
|
larva | + |
Pteromalidae | Mesopolobus mediterraneus (Mayr, 1903) |
|
pupa | - |
Pteromalus chrysos Walker, 1836 |
|
pupa | - | |
Pteromalus semotus Walker, 1834 |
|
pupa | - | |
Tachinidae | Phytomyptera nigrina (Meigen, 1824) |
|
larva | - |
Phytomyptera vaccinii Sintenis, 1897 |
|
larva | - | |
Trichogrammatidae | Trichogramma bourarachae Pintureau & Babault, 1988 |
|
egg | - |
Trichogramma brassicae Bezdenko, 1968 |
|
egg | - | |
Trichogramma cordubensis Vargas & Cabello, 1985 |
|
egg | - | |
Trichogramma dendrolimi Matsumura, 1926 |
|
egg | - | |
Trichogramma euproctidis (Girault, 1911) |
|
egg | + | |
Trichogramma minutum Riley, 1871 |
|
egg | - | |
Trichogramma oleae Voegele & Pointel, 1979 |
|
egg | + | |
Trichogramma pretiosum Riley, 1879 |
|
egg | - |
Family Braconidae
Apanteles xanthostigma (Haliday, 1834)
Material examined: 11 ♀, Messara (Crete) (35°2'20"N, 24°50'54"E), 16–23.06.2017, captured in McPhail trap device.
Chelonus (Microchelonus) elaeaphilus (Silvestri, 1907)
Material examined: 4 ♀, 4 ♂, Messara (Crete) (35°2'20"N, 24°50'54"E), 09–16.06.2017, captured in McPhail trap device.
Chelonus (Parachelonus) pellucens (Nees, 1816)
Material examined: 6 ♀, Messara (Crete) (35°2'20"N, 24°50'54"E), 09–16.06.2017, captured in McPhail trap device.
Glyptapanteles vitripennis (Curtis, 1830)
Material examined: 2 ♀, 7 ♂, Messara (Crete) (35°2'20"N, 24°50'54"E), 23–30.06.2017, captured in McPhail trap device.
Family Eulophidae
Pnigalio mediterraneus Ferrière & Delucchi, 1957
Material examined: 12 ♂, Heraklion, Voutes, (Crete) (35°15'54"N, 25°03'26"E), 15.03.2017 (date of host collection). Host: Prays oleae on Olea europaea var. koroneiki.
Family Eupelmidae
Eupelmus urozonus Dalman, 1820
Material examined: 8 ♀, 12 ♂, Heraklion, Voutes, (Crete) (35°15'54"N, 25°03'26"E), 15.03.2017 (date of host collection). Host: Prays oleae on Olea europaea var. koroneiki.
Family Ichneumonidae
Diadegma armillatum (Gravenhorst, 1829)
Material examined: 3 ♀, 5 ♂, Messara (Crete) (35°2'20"N, 24°50'54"E), 16–23.08.2017, captured in McPhail trap device.
Exochus lentipes Gravenhorst, 1829
Material examined: 6 ♀, 8 ♂, Messara (Crete) (35°2'20"N, 24°50'54"E), 16–23.09.2017, captured in McPhail trap device.
Microgastrinae is one of the largest subfamilies of Braconidae with about 2,000 described species worldwide (
The subfamily Cheloninae is formed by more than 1,300 species belonging to 15 genera, thus constituting a quite large part of Braconidae (
Although Eupelmidae is a relatively small family with approximately 1000 species, the genus Eupelmus Dalman, 1820 is a large taxon containing more than 300 species (
The genus Diadegma Förster, 1869 constitutes a large group of Ichneumonid wasps with more than 200 known species worldwide (
Our original findings on associated parasitoids of P. oleae and the compiled information revealed could trigger further studies that deal with the management of this noxious insect species in the target area from a biological control point of view. The identified parasitoid spectrum was broad, despite the short interval of obtaining the data, indicating a potential positive impact of natural enemies to P. oleae, an issue however that merits further field efforts. Last but not least, given that C. pellucens is identified as a new member of the entomofauna of Greece during the present first attempt to record the beneficial parasitoids in olive orchards in Crete, we may expect that additional parasitoid species may occur in this agroecosystem.
We thank Martin Schwarz (Biologiezentrum, Linz, Austria) for consultation on the identification of Diadegma specimens.