Research Article |
Corresponding author: Paulo H. C. Corgosinho ( pcorgo@gmail.com ) Academic editor: Danielle Defaye
© 2018 Paulo H. C. Corgosinho, Terue C. Kihara, Nikolaos V. Schizas, Alexandra Ostmann, Pedro Martínez Arbizu, Viatcheslav N. Ivanenko.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Corgosinho PHC, Kihara TC, Schizas NV, Ostmann A, Arbizu PM, Ivanenko VN (2018) Traditional and confocal descriptions of a new genus and two new species of deep water Cerviniinae Sars, 1903 from the Southern Atlantic and the Norwegian Sea: with a discussion on the use of digital media in taxonomy (Copepoda, Harpacticoida, Aegisthidae). ZooKeys 766: 1-38. https://doi.org/10.3897/zookeys.766.23899
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Aegisthidae is one of the most abundant and diverse families of harpacticoid copepods living in deep-sea benthos, and the phylogenetic relationships within the family are in state of flux. Females of two new deep-water species of harpacticoid copepods belonging to the Hase gen. n. (Aegisthidae: Cerviniinae) are described. The first taxonomic description of marine copepod species based on the combined use of interference and confocal microscopy for the study of the habitus and dissected appendages is presented here. CLSM (Confocal Laser Scanning Microscopy) is a non-destructive method, comparable in quality to SEM (scanning electron microscopy) at the same magnifications. To observe and reconstruct in detail the habitus and dissected appendages, whole specimens and dissected parts were stained with Congo Red, mounted on slides with glycerine for CLSM and scanned under three visible-light lasers. Hase lagomorphicus gen. et sp. n. and Hase talpamorphicus gen. et sp. n. were collected from the sediments of the Southern Atlantic and the Norwegian Sea, from 2270 m and 5468 m depths, respectively. Hase gen. n. is included within Cerviniinae based on the caudal rami which are relatively divergent. Hase gen. n. is the sister taxon of Cerviniella based on the following synapomorphies: sturdy body, exopodites 1–3 of pereopods 1–3 heavily built, transformed into digging limbs, with strong outer and distal spines/setae, two-segmented endopod on the pereopods 2 and 3, and a reduced pereopod 5. Compared to Cerviniella, Hase gen. n. exhibits a more developed armature on the pereopod 1, which has outer and distal elements transformed into strong and long spines vs. stiff setae on Cerviniella.Hase gen. n. has one or two strong and long spines on the inner margin of the exopodite 3 of pereopod 4 and pereopod 5 is fused to the somite, ornamented with three distal setae. The telson of Hase gen. n. is subquadratic, and the furca is among the shortest yet described for Aegisthidae. The new species differ in a number of diagnostic characters, three of which are: a) the somite bearing pereopods 3 and 4 with latero-distal spiniform processes in H. talpamorphicus gen. et sp. n. but smooth in H. lagomorphicus gen. et sp. n., b) antenna is armed with three stout spines on the lateral inner margin of the exopod in H. talpamorphicus gen. et sp. n. and two proximal setae in H. lagomorphicus gen. et sp. n., and c) pereopod 4 exopodite 3 has two long and strong spines on the inner margin in H. lagomorphicus gen. et sp. n. and one spine in H. talpamorphicus gen. et sp. n. The high quality of CLSM images should foster discussion about the use of high quality digital images as type or as part of the type series in zoological studies, especially when studying rare and small macrofaunal and meiofaunal taxa.
Arctic biodiversity, Cerviniella, deep-sea biodiversity, digital taxonomy, meiofauna, Paracerviniella , Tropical Atlantic biodiversity
Aegisthidae Giesbrecht, 1893 is one of the most abundant and diverse families of harpacticoid copepods living in deep-sea plankton and benthos (
The phylogenetic relationships within the family Aegisthidae are in state of flux. According to
The paper describes two new species of copepod crustaceans designated to a new genus of the subfamily Cerviniinae (Harpacticoida: Aegisthidae) found in the deep waters of Southern Atlantic and Norwegian Sea. This is the first formal description of a marine copepod species based on combined use of interference and confocal microscopy in study of dissected appendages and the genital field. The methods for the acquisition of 3D rendered images are described by
The copepods were extracted from sediment samples of three scientific cruises of the Research vessel (RV) “Meteor”. Sediment samples (5127–5455 m depth) were collected by a multi corer (MUC) during the DIVA-1 expedition of the RV “Meteor” (Cruise No. M48/1) to southeast Atlantic Ocean in July–August 2000. During DIVA-2 Expedition of the RV “Meteor” (Cruise No. M63/2), samples were taken by a MUC in the equatorial east Atlantic at depths >5000 m. Additional samples were collected by a box corer (BC) during the Overflow, Circulation and Biodiversity Expedition of the RV “Meteor” (Cruise No. M85/3) 307–2749 m deep (Fig.
Sampling stations of the “Meteor” cruises. Abbreviations: BC – Box Corer, MUC – Multi Corer.
Species | Cruise | Station | Date | Gear | Latitude, Longitude | Depth [m] | Temperature [°C] | Salinity [‰] | No. of specimens |
---|---|---|---|---|---|---|---|---|---|
Hase lagomorphicus gen. et sp. n. (paratype CV and holotype) | M48/1 | 330 | 17/07/2000 | BC | 19°06.986'S, 003°52.017'E | 5468 | 2.4941 | 34.7779 | 1 adult ♀, 1 subadult copepodite V |
Hase lagomorphicus gen. et sp. n. (paratype) | M63/2 | 105 | 24/03/2005 | BC | 00°37.266'N, 006°28.119'W | 5173 | 2.1087 | 34.5436 | 1 adult♀ |
Hase talpamorphicus gen. et sp. n. (paratype CV and adult) | M85/3 | 1151 | 17/09/2011 | MUC | 69°05.60'N, 009°56.01'W | 2270 | -0.7518 | 34.91 | 1 adult ♀, 1 subadult copepodite V |
Hase talpamorphicus gen. et sp. n. (holotype) | M85/3 | 1164 | 18/09/2011 | MUC | 67°35.28'N, 006°57.48'W | 2403 | -0.82 | 34.91 | adult ♀ |
For taxonomic studies, specimens were stained with Congo Red and mounted on slides for confocal laser scanning microscopy (CLSM) following
The habitus was drawn from whole specimens temporarily mounted in slides with glycerine, adhesive plastic discs were used to support the cover slip and prevent destruction of the specimen (Kihara and Falavigna da Rocha 2009). After CLSM microscopy, specimens were dissected under a Leica MZ12.5 (Leica, Wetzlar, Germany). Dissected parts were mounted on slides using glycerine as mounting medium, and preparations were sealed with transparent nail varnish. Drawings were made under a Leica DMR microscope equipped with Nomarsky interference contrast and a drawing tube at 400× and 1000× magnification (Leica, Wetzlar, Germany). Final illustrations were “digitally inked” using Adobe Illustrator CS6 (DIVA-1 and DIVA-2 species) or free hand inked (IceAGE species).
The terms ‘furca’ and ‘telson’ are used according to
The following abbreviations are used in the text:
A1 antennule;
Ae aesthetasc;
A2 antenna;
enp endopod;
enp-1 (2,3) proximal (middle, distal) segment of endopod;
exp exopod;
exp-1 (2,3) proximal (middle, distal) segment of exopod;
Md mandible;
Mx1 maxillule;
Mx2 maxilla;
Mxp maxilliped;
P1–P6 first to sixth pereopods;
pl plesiomorphy;
sy synapomorphy.
Hyphen (i.e., “-”) between figure numbers, structures, number of spines and setae, etc. indicates all between and is inclusive (ex: P1-P5 = P1, P2, P3, P4, and P5; A-C = A, B and C; etc.)
The type material is deposited at the Forschungsinstitut und Naturmuseum Senckenberg (SMF) in Frankfurt, Germany.
Female body sturdy with clear distinction between prosome and narrower urosome. Prosome 5-segmented, with cephalosome and four free pedigerous somites. Cephalosome with minute spinules covering surface and anastomosing reticulation towards rostrum and along margins; posterior margin slightly serrate. Pedigerous somites with reticulation along ventroposterior margins; lateral margins of third and fourth pedigerous somites smooth or expanded posteriorly forming hook-like projections laterally; posterior margins serrate. Urosome 5-segmented, comprising P5 bearing somite, genital double-somite, two free abdominal somites, and telson. Genital double-somite and two free abdominal somites with hook-like projections ventrolaterally. Genital double-somite original segmentation indicated by transverse, serrate surface ridge with reticulation and sensilla dorsal and laterally, completely fused ventrally; genital field with copulatory pore located in median depression; gonopores covered by operculum derived from sixth legs and by anteriorly directed flap arising from somite wall; P6 fused genital opercular plate armed with two setae. Telson with well-developed anal operculum; large anal opening with folded and reticulated cuticle; surface ornamentation consisting of pair of sensilla dorsally, minute spinules and pair of pores ventrally; ventral posterior margin with minute setules. Furca symmetrical; approximately 3.4× as long as maximum width; distinctly convergent. Each ramus with seven setae: setae I-III not inserted close to each other; seta I proximal, laterally inserted, spiniform and bipinnate; seta II median, dorsally inserted, spiniform, and bipinnate; seta III subdistal, laterally inserted, spiniform and bipinnate; setae IV and V distally inserted, bipinnate and fused basally; seta VI distally inserted, minute and naked; seta VII dorsally inserted, close to seta III, tri-articulate at base and pinnate.
Rostrum fused to cephalic shield; tip rounded, with tuft of spinules along distal margin or slightly bifid and smooth; with pair of sensilla near apex. A1 7-segmented, proximal segments 1–3 cylindrical or subcylindrical; distal segments flattened. Segment I the longest; segment III with aesthetasc fused basally to single seta and set on distinct pedestal; segment VII with aesthetasc fused basally to one seta. Armature formula: I-[1], II-[8-9 elements], III-[10-12 + (1 + Ae)], IV-[3], V-[2], VI-[2], VII-[6-7 + (one naked + Ae)]. A2 3-segmented, comprising cylindrical coxa and allobasis, and 1-segmented flattened enp. Coxa small. Basis and enp-1 fused, forming elongate allobasis and with abexopodal seta. Enp medial armature four elements, apical armature 3–4 spines, one seta, and three fused elements. Exp 4-segmented; armature formula: I-[2], II-[1], III-[1], IV-[2-3].
Md. Coxa with well-developed musculature, gnathobase curved inwards, bearing several multicuspid teeth and single seta on inner distal margin. Palp well developed, comprising basis, enp and exp. Basis with four setae. Enp 1-segmented with three lateral setae and 6–7 apical setae. Exp 4-segmented; armature formula: I-[2], II-[1], III-[1], IV-[2]. Mx1. Praecoxa with row of spinules; arthrite well developed and with 13–14 elements. Coxa endite cylindrical, bearing 5–6 setae distally; epipodite absent. Basis and enp fused; basis with eleven setae; enp incorporated into basis, represented by 2–3 naked setae. Exp 1-segmented, with 2–3 setae. Mx2 comprising syncoxa fused to allobasis, and 4-segmented enp. Syncoxa/allobasis with four endites; proximal coxal endite with five pinnate setae; distal coxal endite almost completely incorporated into syncoxa, with three setae; proximal basal endite with three setae; distal basal endite with two setae and one spine. Enp-1 endite forming strong claw; accessory armature consisting of two setae, one or two spines and zero or one tube pore; armature of fused enp-2 represented by three or four elements. Free enp 3-segmented; armature formula: I-[claw; 3–4 spines/setae; 0–1 tube pore], II-[3-4], III-[2], IV-[2-3], V-[3-4]. Mxp with elongated syncoxa, strong basis and 2-segmented enp; syncoxal endites represented proximal to distal by two elements, 3–4 elements, and 2–3 elements; basal endite represented by two elements. Enp with armature formula: I-[2], II-[four elements].
Pereopods biramous; exp and enp flattened, bent inwards, especially on P1 and P2. Praecoxa without ornamentation. Coxa without ornamentation (P1) or ornamented (P2-P4). Basis with (P1 and P2) or without (P3 and P4) one seta on outer proximal corner, with one seta on inner distal corner of P1. Exp 3-segmented. Enp 3-segmented (P1), 2-segmented (P2 and P3) and 1-segmented (P4). P5 1-segmented, pointing outwards, fused to supporting somite. Exp with three elements. P1-P4 spine and setal formulae as follows:
The generic name, Hase, from German, means “hare”, and refers to the very superficial resemblance of the new species to a hare or rabbit. Gender masculine.
Hase lagomorphicus sp. n., by present designation.
Holotype, adult female dissected on six slides (reg. no. SMF 37130/1-6), from DIVA-1 (M48/1, 330). Paratype, adult female (incomplete) dissected into three slides (reg. no. SMF 37131/1-3), from DIVA-2 (M63/2, 105). Paratype 2, subadult copepopid stage V (CV) dissected into five slides (reg. no. SMF 37132/1-5), from DIVA-1 (M48/1, 330).
Angola Basin (DIVA-1 cruise M48/1, 330) (Fig.
The specific epithet is built by combining the ancient Greek lexemes λαγός (lagós), meaning hare, and μορφώ (morphó), “the Shapely One”.
Female. Total body length 730 μm (paratype 1) and 735 μm (holotype) (N = 2; mean = 732.5 μm). Largest width measured at posterior margin of P2-bearing somite: 292 μm (paratype 1) and 295 μm (holotype) (N = 2; mean = 293.5 μm).
Body (Fig.
Urosome (Figs
Genital double-somite (Figs
Telson (Figs
Furca (Figs
Rostrum (Fig.
A1 (Figs
Armature formula: I-[one pinnate], II-[six naked + two unipinnate], III-[ten naked + (one naked + Ae)], IV-[one bipinnate + two naked], V-[two naked], VI-[one unipinnate + one naked], VII-[two naked, three bipinnate + one unipinnate + (one naked + Ae)].
A2 (Figs
Md (Fig.
Hase lagomorphicus gen. et sp. n. Holotype (female) (M48/1, 330, DIVA-I): AMd gnathobasis; a1- mandibular palp with basis, exp and enp; a2- exp; a3- enpBMx2 syncoxa, allobasis, and first enp without claw; b1 enp1 with claw and accessory spines, and 2ndenp (fused) in lateral view; b2 enp-1 with claw, and enp-2 to enp-5; b3 upper view of enp-1 to enp-5; b4 upper view of enp-2; b5-b7 upper view of enp-3 to enp-5 CMx1 with unarmed coxa, basis, enp and exp; c1 and c2- coxa; c3 basis with incorporated enp; c4- exopod DMxp.
Mx1 (Figs
Mx2 (Figs
Mxp (Figs
Pereopods (Figs
Exp | Enp | |
---|---|---|
P1 | I, 1; I, 1; II, II+1, 1 | 0, 1; 0, 1; I, 2, 2 |
P2 | I, 1; I, 1; II, II+1, 2 | 0, 1; I (broken), 2, 1 |
P3 | I, 1; I, 1; II, II+1, 2 | 0, 1; I, 2, 0 |
P4 | I, 1; I, 1; II, II+1, II | 0, 2, I |
P5 (Fig.
Male unknown.
Angola and Guinea basins, Atlantic Ocean.
In the subadult CV, enp is 3-segmented on P2-P4; exp-3 of P4 with 8 elements (Fig.
Holotype female dissected on 21 slides (reg. no. SMF 37133/1-21) from station 1164, multi corer 9. Undissected paratypes: one female (reg. no. SMF 37134/1) from station 1151, MUC 12 and one subadult copepopid stage V (CV) (reg. no. SMF 37135/1) from station 1151, MUC 10. All specimens were collected during the Overflow, Circulation and Biodiversity Expedition of the RV “Meteor” (Cruise No. M85/3).
Norwegian Sea (IceAGE cruise M85/3, 1164) (Fig.
The specific epithet is built by combining the Latin talpa, meaning a mole, and the ancient Greek lexeme μορφώ (morphó), “the Shapely One”.
Female. Total body length 986.7 μm (holotype) and 1000.0 μm (paratype) (N = 2; mean = 993.4 μm). Largest width measured at posterior margin of P2-bearing somite: 400.0 μm (holotype) and 437.5 μm (paratype) (N = 2; mean = 418.7 μm).
Body (Fig.
Urosome (Figs
Genital double-somite (Figs
Telson (Figs
Furca (Figs
Rostrum (Fig.
A1 (Figs
Armature formula: I-[one pinnate], II-[four naked + three bipinnate + two missing elements], III-[eleven naked + one bipinnate + (one naked + ae)], IV- [three naked], V-[two naked], VI-[two naked], VII- [three naked, three pinnate + (one naked + ae)].
A2 (Figs
Md (Figs
Mx1 (Figs
Mx2 (Figs
Mxp (Figs
Pereopods (Figs
Exp | Enp | |
---|---|---|
P1 | I, 1; I, 1; II, II+1, 1 | 0, 1; 0, 1; I, 2, 2 |
P2 | I, 1; I, 1; II, II+1, 2 | 0, 1; I, 2, 1 |
P3 | I, 1; I, 1; II, II+1, 2 | 0, 1; I, 2, 0 |
P4 | I, 1; I, 1; II, II+1, I | 0, 2, 0 |
P5 (Figs
Male unknown.
P4exp-3 bears two outer spines in the normal condition (formula [II, II+1, I], two females). However, in one female, P4exp-3 displayed [II, II+1, I] on one side and [I, II+1, I] on other side (Fig.
In the juvenile CV, segmentation and armature of P1-P4 as in Hase lagomorphicus gen. et sp. n.
According to
Hase gen. n. can be included within Aegisthidae on account of the above mentioned apomorphies 5, 6, and 10. Species of Aegisthinae are derived Cerviniinae and Cerviniopseinae (
Hase gen. n. has an antenna with four-segmented exp and could be included within both Cerviniinae and Cerviniopseinae. However, Cerviniinae and Cerviniopseinae are to date separated according to the degree of divergence of the caudal rami (see
In Cerviniella the whole exopod or the exopodite-2 and 3 are fused on the P1-P3 (sy), keeping the inner and outer armature of the original segments, the endopodite of P1 is never 3-segmented (sy) and the P4 undergoes a further reduction both in segmentation and/or armature of the exopod and endopod (sy) (viz.
Interestingly, the same morphology of the P5, telson and furca is depicted by
Most of these characters are not informative enough to allow the inclusion of Paracerviniella within any monophyletic clade within the Aegisthidae. With exception of the clawed spine present on the endopodites of P1 and P2, the P5 morphology and armature and maybe body ornamentation, the remaining characters are gender-linked or plesiomorphic within the family. In addition, the illustration of some characters that could be informative, such as the mouthparts, is insufficient. Considering this and on the absence of females, we cannot address in what degree Hase gen. n. and Cerviniella are phylogenetically related to Paracerviniella.
The main differences in morphology of H. lagomorphicus gen. et sp. n. and H. talpamorphicus gen. et sp. n. are summarized on Table
The shape of the gonopores and the position of the copulatory pore as they are depicted by the CLSM (Figs
Distinctive characters of Hase lagomorphicus gen. et sp. n. and Hase talpamorphicus gen. et sp. n.
Hase lagomorphicus gen. et sp. n. | Hase talpamorphicus gen. et sp. n. | ||
---|---|---|---|
Lateral margins of 3rd and 4th pedigerous somites | Smooth (Fig. |
Expanded posteriorly forming hook-like projections laterally (Fig. |
|
Rostrum | Tip | Rounded; with tuft of spinules along distal margin and with pair of sensilla near apex. (Fig. |
Slightly bifid; with tuft of spinules along distal margin, with pair of sensilla and midventral tube-pore near apex (Fig. |
A1 | Segment II | 8 setae (Fig. |
7 setae + 2 missing elements (Fig. |
Segment III | 10 setae + (1 seta+ ae) (Fig. |
12 setae + (1 seta + ae)] (Fig. |
|
A2 | Enp medial armature | 4 setae and 1 spine (Fig. |
1 seta and 3 spines (Fig. |
Enp apical armature | 3 spines, 1 seta and 3 elements fused basally (2 long setae medially unipinnate, and 1 smooth seta) (Fig. |
4 spines, 1 seta and 3 elements fused basally (1 bipinnate seta, 1 unipinnate seta and 1 small flattened seta) (Fig. |
|
Exp-4 | 2 setae (Fig. |
3 setae (Fig. |
|
Md | Enp | 3 lateral and 6 apical setae (Fig. |
3 lateral and 7 apical setae (Fig. |
Mx1 | Arthrite | 2 setae on anterior surface, 7 spines along distal margin, 4 setae on the aboral margin (Fig. |
2 setae on anterior surface, 7 spines and 3 setae along distal margin, 2 setae on posterior surface. (Fig. |
Coxa endite distal armature | 5 setae (Fig. |
6 setae (Fig. |
|
Enp incorporated to basis | 2 setae (Fig. |
3 setae (Fig. |
|
Exp | 3 setae (Fig. |
2 setae (Fig. |
|
Mx2 | Enp-1 endite | 2 setae, 1 spine and 1 claw-like spine (Fig. |
2 setae, 1 spine and 1 tube pore (Fig. |
Enp-2 | 3 setae (Fig. |
3 setae and 1 spine (Fig. |
|
Enp-5 | 3 setae (Fig. |
4 setae (Fig. |
|
Mxp | Syncoxal endites (proximal to distal) | 1 seta and 1 spine, 2 setae and 1 spine, and 1 seta and 1 spine (Fig. |
1 seta and 1 spine, 3 setae and 1 spine, and 2 setae and 1 spine (Fig. |
Enp-2 | 1 spine and 3 setae (Fig. |
2 spines and 2 setae (Fig. |
|
P4 | Exp-3 | II, II+1, II (Fig. |
II, II+1, I (Fig. |
Enp | 0, 2, I (Fig. |
0, 2, 0 (Fig. |
|
P5 | Exp | 1 seta, 1 spine and 1 missing element (Fig. |
3 setae (Fig. |
Genital Field | Copulatory pore | Slightly covered by a proximal flap, pointing posteriorly, located in a soft median depression (Fig. |
Completely visible, not covered by a proximal flap as observed for the previous species, located in a well-developed median depression (Fig. |
Gonopores | Covered by medially depressed operculum (Fig. |
Covered by a straight operculum (Fig. |
There are some important differences among the scanning microscopy systems that produce high quality imaging, especially regarding to the subsequent fate of the specimens and the resolution limits. Some image systems (e.g., SEM) inevitably destroy type specimens; CLSM is highly desirable in this aspect as the studied specimen remains intact. According to
Recent papers have highlighted the importance of image acquisition in taxonomy (e.g.,
This contribution highlights the diversity of exquisite bauplans in deep-sea copepods and the broad distribution of a meiobenthic crustacean genus in the Atlantic Ocean basins. Additionally, it is a showcase on how confocal microscopy can assist in providing a better and more accurate description of small macrofaunal and meiofaunal organisms. We favour the inclusion of digital media at least as a component of the type series and we encourage the discussion for setting standards for such data. Additional studies and sampling effort must be continued to find the male of the genus Hase gen. n. to improve comparisons with Paracerviniella and Cerviniella.
We are indebted to the Census of Abyssal Marine Life CeDAMar, which financially supported the senior author with a postdoctoral fellowship program for the study of deep-sea harpacticoids from 2007 to 2009. CeDAMar received financial support from the Alfred Sloan Foundation as a Census of Marine Life project. The cruises which sampled the species described in this study were financed by the German Research Foundation (DFG). We are indebted to the technical staff of DZMB for sorting the samples and to our secretary Ms. Christa Dohn for her outstanding work and care with Senckenberg guest scientists. We are also indebted to Prof. Dr. Wonchoel Lee, the editor of Zookeys Prof. Danielle Defaye and an anonymous reviewer for their valuable comments. Taxonomic literature and data analysis were partially supported by RFBR, research project (grant no. 15-54-78061).