Description of immature stages and biological notes of Cassidispa relicta Medvedev, 1957, a newly recorded species from China (Coleoptera, Chrysomelidae, Cassidinae, Hispini)

Chengqing Liao; Zhilin Zhang; Jiasheng Xu; Charles L. Staines; Xiaohua Dai

doi:10.3897/zookeys.780.23280

Abstract

The first instar and mature larva and pupa of Cassidispa relicta Medvedev, 1957, a newly recorded species from China, are described and figured. The chaetotaxy of the head, mouthparts, legs, and dorsal and ventral surfaces of the body is described. This is the first detailed description of immatures in the genus Cassidispa. Diagnostic characters of this species are compared with other described immatures of some Hispini genera. Biological notes on C. relicta, such as host plants, feeding patterns of adults, structure of larval mines and life history, are also presented.

Keywords

Cassidinae , Cassidispa , chaetotaxy, Hispini , immature stages, leaf-mining insects, morphology

Introduction

Cassidispa Gestro, 1899 is a leaf beetle genus belonging to the tribe Hispini Gyllenhal, 1813 (Chrysomelidae: Cassidinae), with eight species occurring in China, Russia, Angola, Democratic Republic of Congo and Zimbabwe (Staines 2015). Four species are previously recorded from China: C. bipuncticollis Chen, 1941, C. femoralis Chen & Yu, 1976, C. maderi Uhmann, 1938 and C. mirabilis Gestro, 1899 (Chen et al. 1986; T’an 1993; Hua 2002; Staines 2015). Adults of the genus Cassidispa can be distinguished from all other genera of Hispini by the anterior margin of the pronotum without spines, by the pronotum and elytra with broadly expanded margins, and by the antennae having nine antennomeres (Chen et al. 1986). The main diagnostic characters between Cassidispa and the similar genus Platypria Guérin-Méneville, 1840 are the lateral margins of pronotum expanded from base to anterior angle with irregular translucent patches, the continuous expanded margins of the elytra, the elytra with very low and obtuse tubercles, never with spines, and the lateral margins of elytra with numerous small spines not with long ones (Chen et al. 1986).

There is little published biological information on Cassidispa species. Hua (2002) listed the host of C. bipuncticollis as Betula spp. (Betulaceae) from China. Recently, we discovered that the larvae of C. relicta mine in the leaves of Betula platyphylla Suk. (Betulaceae) and Ulmus pumila Linn. (Ulmaceae) in Inner Mongolia, China. The species reached outbreak levels in 2016–2017 and became a potential pest of the dominant trees in the area. As Dr. Lukáš Sekerka pointed out, the species is Cassidispa relicta Medvedev, 1957 not C. mirabilis. Both species are superficially similar by predominantly black coloration but they are distinct: C. relicta has generally strongly shiny dorsum (but not as shiny in C. mirabilis); C. relicta has shorter and thicker antennae, explanate margin of pronotum largely black (but yellow in C. mirabilis); C. relicta has anterior spots on explanate margin of elytra almost reaching to humeri (but widely separated from humeri in C. mirabilis); elytra of C. relicta is very distinctly constricted in 3/4 length (but weakly in C. mirabilis) (Lukáš Sekerka, Personal Communications). C. relicta is previously reported in Russia (Medvedev 1957; Staines 2015). Therefore, it is a newly recorded species from China and China hosts all five Asian Cassidispa species now.

In this publication, we describe the larvae and pupa of C. relicta and provide the first detailed report on immature morphology and biological information for the genus Cassidispa.

Materials and methods

All immatures and adults were observed and collected at Shanggaotai Forest Farm (Zhuozi County, Inner Mongolia) from March 2016 to October 2017. Immatures and adults of C. relicta were collected on wild plants and some of them were preserved in anhydrous ethanol. Some adults were pinned in the laboratory (Figs 1–3) and determined using the keys in Chen et al. (1986). Four first-instar larvae, three mature larvae and three pupae were examined morphologically. For microscopic study, the heads of the larvae were separated from the rest of the body, boiled in 10% NaOH solution and cleaned in water before dissecting the mouthparts.

The photos of adults were taken using a Cannon EOS 7D camera with macro lenses; the dissection of heads and mouthparts was made with a Motic SMZ-140 and Olympus SZX2-ILLT stereomicroscope; figures and examination were performed using an Optika B-292 microscope and Cannon EOS 70D camera. Our descriptions of immature stages follow Świętojańska et al. (2006). The terminology of the chaetotaxy of the head follows Borowiec and Świętojańska (2003). All studied material (first-instar larvae, mature larvae, and pupae) and adults were deposited at the Leafminer Group, School of Life and Environmental Sciences, Gannan Normal University (Ganzhou, China).

Figures 1–3.

Cassidispa relicta. 1 Dorsal view 2 Ventral view 3 Lateral view.

Results

Cassidispa relicta Medvedev, 1957

Larva

(Figs 4–17). Length of mature larva 6.1–6.2 mm without head, width 1.6–1.7 mm across pronotum. Length of first instar larva 1.4–1.8 mm without head, width of body 0.7–0.8 mm across pronotum.

Body distinctly flattened dorso-ventrally. Pronotum of first instar larvae slightly wider than abdominal segments; mature larvae widest across abdominal segments IV–V (Figs 6–7, 16–17). Body color of alcohol-preserved larvae yellowish-white with two irregular brown patches on pronotum (paler and without dark markings in first instar larvae), black anterior margin of abdominal segment IX, dark brown spiracles, yellowish-brown triangular patch on prosternum, brown head and legs. Abdomen of live larvae dark brown or black (Figs 31–32).

Body with eight pairs of lateral scoli on abdominal segments (Figs 6–7, 16–17). Lateral scoli short and round, approximately of same length; scoli of segments VI–VII with two small simple lateral branches (first instar larvae without lateral branches as in Figs 4–5). All lateral scoli with two setae apically and one seta ventrally.

Figures 4–5.

Cassidispa relicta, first instar larva. 4 Dorsal view 5 Ventral view.

Granulation of body distinct in all examined specimens including both first instar and mature larvae. Each tergite and sternite (except for sternite VIII) with minute setae on anterior margin; tergites and sternites covered with short pointed setae. Tergites of meso- and metathorax, abdominal segments I–VI and sternites I–VII of abdomen with transverse grooves (Figs 4–7). Sternites of meso- and metathorax with two short transverse grooves medially, very similar in shape to other tergites and sternites. Posterior margin of each transverse groove and anterior margin of pronotum with distinct asperites.

Pronotum with four setae on each lateral margin, five setae on each lateral side, and three setae close to posterior margin (Figs 4, 6). Meso- and metanotum with six minute setae on anterior margin: two pairs at middle and one pair laterally; row of ten setae running across segment; group of six setae laterally. Meso- and metanotum with one seta on slightly visible protuberance laterally. Abdominal tergites I–VII with four setae on anterior margin; two rows of setae running across segment: anterior with two setae, posterior with four setae; three setae close to each spiracle (seta closest to spiracle minute). Abdominal tergite VIII with four minute setae on anterior margin; three rows of setae running across segment: anterior with four setae, median with two setae, and posterior with four setae (between spiracles). Posterior margin of abdominal segment VIII with ten setae: two pairs close to spiracles laterally, three pairs between spiracles medially.

Prosternum with one seta in each anterior angle; one seta laterally at base of leg; and two setae close to posterior margin (Figs 5, 7). Meso- and metasternum with four setae on anterior margin; two rows of setae running across segment medially, both with two setae; and two setae laterally at base of leg. Abdominal sternites I–VII with pair of minute setae on anterior margin medially; with row of six setae running across segment medially; and three setae laterally. Abdominal sternite VIII with row of eight setae across segment anteriorly and two setae posteriorly; with two setae close to each spiracle; four setae along anterior base of anus.

Nine pairs of distinct spiracles (Figs 4, 6, 16): one on thorax and eight on abdomen. Thoracic spiracles distinctly more elevated than abdominal spiracles, diameter of spiracles of abdominal segments I–VII approximately equal, but spiracles of abdominal segment VIII distinctly larger, flattened, slightly elevated.

Figure 6.

Cassidispa relicta, last instar larva, dorsal view.

Head well sclerotized, prognathous, partially retracted into pronotum (Figs 8–9). Epicranial stem absent; median endocarina wide, extending between frontal arms; frontal arms V-shaped, fronto-clypeal suture absent. Clypeus wider than long, without setae and campaniform sensilla. Frons with two short setae (Fd1 and Fc3) and three campaniform sensilla laterally, two setae (Fc1 and Fc2) and one campaniform sensillum between median endocarina and frontal arm, one long seta (Fb4) laterally close to frontal arm; vertex with seven short setae (Fb1, Fb2, Fb3, and V1-4) and four campaniform sensilla (three respectively close to Fb1, Fb2 and V4, one between Fb4 and dorsal stemmata). One long seta (Fa1) on lateral margin close to pronotum, four long pointed setae (Fa2, Fa3, Fa4, and Fa5) close to stemmata. Temporal side with two long setae (T2 close to antenna, and T1 between T2 and ventral stemmata) and two campaniform sensilla.

Figure 7.

Cassidispa relicta, last instar larva, ventral view.

Five stemmata laterally: four dorsal-laterally, one ventrally (Figs 8–9). Antenna with three antennomeres, set in membranous ring (Figure 14). First antennomere stout, approximately as wide as long, with three campaniform sensilla; second antennomere stout, slightly longer than wide, with two campaniform sensilla, one prominent sensory appendix apically, and one stout seta close to third antennomere; third antennomere very short, with long, pointed seta and three peg-like sensilla.

Labrum approximately three times wider than long, anterior margin slightly emarginate (Figs 10–11), dorsal surface with: three setae and one campaniform sensillum laterally; one pair of campaniform sensilla medially; and four stout setae laterally close to anterior margin. Mid- and anterior areas of ventral surface with numerous stout spines; lateral and posterior areas with tiny spines; two irregular groups of few small sensilla medially.

Mandibles heavily sclerotized, with two prominent teeth (Figure 12): anterior distinct and conical, posterior blunt; followed by some tiny teeth. Two long setae very close to each other and one campaniform sensillum.

Maxillae and labium connate (Figure 15). Each stipes (st) with three campaniform sensilla anteriorly. Palpifer (pp) with one small seta apically and one campaniform sensillum laterally. Maxillary palp (mp) with two palpomeres: first palpomere stout, second palpomere with group of small peg-like sensilla at apex. Mala (mal) with six long pointed setae and one short seta apically, and two setae subapically. Hypopharynx (hyp) covered with numerous small spines. Labial palp (lp) with one palpomere, with group of small peg-like sensilla at apex. Prementum (pre) with two campaniform sensilla anteriorly. Postmentum (post) with two pairs of short setae at base and two campaniform sensilla medially.

Legs oblong, consisting of three segments: coxa, femur, and tibiotarsus (Figure 13). Tibiotarsus armed apically with claw. Coxa with four setae along base on internal surface, and one seta dorsally and two setae on external surface. Femur with five setae and four campaniform sensilla on basal half, and six long setae on apical half. Tibiotarsus with nine long pointed setae and two campaniform sensilla: two setae at middle laterally, six setae around claw apically, one seta and two sensilla above claw. Base of claw with distinct pulvilli.

Figures 8–15.

Cassidispa relicta, larva. 8 Dorsal view of head: cs – campaniform sensilla 9 Ventral view of head 10 Dorsal view of labrum 11 Ventral view of labrum 12 Mandible 13 Leg 14 Antenna 15 Maxillae and labium, ventral. Abbreviations: hyp – hypopharynx; mal – mala; mp – maxillary palp; pp – palpifer; lp – labial palp; pre – prementum; post – postmentum; st – stipes.

Pupa

(Figs 18–21). Length of pupa 5.2 mm, width 1.7 mm across base of pronotum and 2.5 mm across abdominal segment IV without lateral scoli.

Body flattened dorso-ventrally, elongate-oval. Color of live as well as alcohol-preserved pupa brownish yellow, mesothorax with two dark markings at base of wings, apex of abdomen dark brown (Figs 18–19).

Body, especially abdomen, distinctly granulate (Figs 18–21). Abdominal tergites I–III with two short transverse grooves medially. Abdominal tergites IV–VII each with long transverse groove. Tergites of abdominal segments I–VII with several small tubercles around grooves. Tubercles of tergites VI–VII arranged as row close to posterior margin. Sternites of abdominal segments IV–VII each with two transverse ridges close to posterior margin.

Head visible in dorsal view (Figs 18, 20). Prothorax trapezoidal in shape. Thorax without lateral scoli. First abdominal segment without lateral scoli. Segments II–V with single simple scolus laterally and small low tubercle anteriorly armed with pointed seta. Abdominal segment VI with two lateral scoli. Segments VII–VIII each with three lateral scoli. Each scolus apically armed with one seta. Posterior lateral scolus of segments VI–VIII with one lateral branch directed posteriorly without setae. Segment VIII additionally with two broad, flattened processes on posterior margin; each with six setae apically.

Head with three pairs of setae laterally and four setae anteriorly (Figure 20). Pronotum with group of nine setae laterally. Mesonotum with three pairs of setae medially. Metanotum with oblique row of four setae on each side medially. Abdominal tergites I–VII with two rows of setae running across segment: anterior with two setae, posterior with four setae; two setae close to each spiracle. Abdominal tergite VIII with three rows of setae running across segment: anterior with four setae, median with four setae, and posterior with two setae medially; one seta very close to each spiracle.

In ventral view (Figure 21), head with group of 14 setae: two setae anteriorly, four setae medially, six setae posteriorly at base of labrum, and two setae at base of mandibles. Tarsus of leg apically armed with one pointed seta. Visible abdominal sternites IV–VII with four setae on anterior margin, row of six setae medially, two setae laterally, and one seta at base of lateral scoli ventrally. Abdominal sternite VIII with rows of six setae anteriorly and six setae along anterior base of anus.

Abdominal segments each with one pair of spiracles (Figure 21). Spiracles of segment I smaller than others, spiracles of segments II–IV and VI–VII similar in shape but approximately twice as large as spiracles of segment I. Spiracles of segment VIII not elevated, oblong. Spiracles of segment V prominent, elongated into cylindrical appendage (respiratory horns), directed posteriorly.

Biological notes

The biology of the genus Cassidispa is poorly known. There is only one species having confirmed host plant record. Hua (2002) reported C. bipuncticollis to be associated with Betula (Betulaceae), but it is just a list even without some evidences. Our rearing of C. relicta on Betula and some other trees indicates that there might be more larval host associations for the genus. Additionally, the immature stages of the genus Cassidispa were completely unknown and this paper was the first study about it.

The larvae of C. relicta were found mainly mining in the leaves of Betula platyphylla (Figs 22–25, 27–34) and occasionally mining the leaves of some other trees, such as Ulmus pumila Linn. (Ulmaceae) (Figure 26), Populus davidiana Dode, P. cathayana Rehd. (Salicaceae) and Armeniaca sibirica (L.) Lam. (Rosaceae).

The life cycle of C. relicta is univoltine based on our field observations throughout 2016–2017. C. relicta overwinters as a mature larva until the temperature rises and the soil thaws in the spring. The mature larvae break dormancy and pupate in the fallen leaves in early April (Figs 33–34). The pupal stage lasts about one month. Adults emerge in late May (the freshly emerged adults are mostly white, with antenna and legs brown, and disc of pronotum black) and feed on the mesophyllic tissue of the upper surface of host leaves (Figs 22–24). The feeding pattern of the adults is usually irregular and may densely cover the leaves (Figs 22–25, 28–32). In early June, the adults start to copulate (Figs 23–24), and fertilized females lay an egg within a shallow hole which was chewed on the lower leaf surface, and then covered with feces (Figs 25–26). Adults have no apparent feeding preferences for young or old leaves, but females generally do not oviposit on new leaves. Females oviposit eggs scatteredly in a one-by-one way (Figs 24–26, 28). The newly deposited eggs are usually milk-white and translucent and its covered feces turn brown over time (Figs 25–26, 28). Sometimes, females will lay some eggs on the tree trunk, perhaps when they could not find any suitable leaves (Figure 27). The egg stage lasts about 20 days. A freshly hatched larva bores into the mesophyllic tissue and forms a large irregular mine with a resting place (dark color) surrounding the oviposition point (Figure 29). The larvae deposit their feces in their own mine (Figs 29–32). Each younger larva has its own absolute mine at the early stage which will combine with other mines on the same leaf over time (Figs 29–32). One leaf usually has three larvae or, in an outbreak, up to seven larvae. If one leaf does not likely provide enough food for these larvae to complete their development, they leave their original mine, migrate to a new leaf, and construct a new mine (Figs 31–32). The final larval mine is a large irregular blotch type, almost without any mesophyllic tissue left (Figs 30–32). The mined leaves gradually become yellow and may dry-up or abscise early. In early October the mature larvae leave their mine to enter a fresh uneaten area of the same leaf to construct a pupal mine (Figs 33–34). The mature larvae of C. relicta does not directly go into pupation like other leaf-mining hispines (Świętojańska and Kovac 2007; Lee et al. 2009; Liao et al. 2014; Liao et al. 2018) but into a long dormant period for overwintering. However, it is a shortcoming we did not perform the larval instar observations.

Discussion

General morphology of the larva and pupa of C. relicta is typical for species of leaf-mining Hispini. The immature stages of Hispini beetles have been reported on some species, such as Acmenychus inermis (Zubkoff, 1833) (Medvedev 1968), Dactylispa setifera (Chapuis, 1877) (Chen et al. 1986), D. xanthopus (Gestro, 1898) (as D. chinensis Weise, 1905), D. doriae Gestro, 1890, D. chaturanga Maulik, 1919, D. xanthospila Gestro, 1890 (Zaitsev 2012), D. ignorata Uhmann, 1953 (as D. chapuisi (Gestro, 1890)), D. rufiventris (Kraatz, 1895) (Maulik 1932), D. feae (Gestro, 1888) (as D. flavomaculata Uhmann, 1930), D. issiki Chûjô, 1938 (Fukuda and Kurosa 1959), D. higoniae (Lewis, 1896), D. subquadrata (Baly, 1874) (Yano 1965), D. hystrix (Duvivier, 1891) (Paulian 1949), D. cladophora (Guérin-Méneville, 1841), D. nemoralis (Gestro, 1897), D. vethi (Gestro, 1906) (Uhmann 1956), D. callosa Uhmann, 1935 (Uhmann 1962), Dicladispa armigera (Olivier, 1808) (Chen et al. 1986; Kimoto and Takizawa 1994; Lee and Cheng 2010), Dicladispa testacea (Linnaeus, 1767) (Świętojańska et al. 2014), Hispa atra Linnaeus, 1767 (Grandi 1935), and Platypria erinaceus (Fabricius, 1801) (as P. andrewesi Weise, 1904) (Uhmann 1957), P. kapauku Gressitt, 1957 (as P. linnei Weise, 1905) (Gressitt 1963), P. melli Uhmann, 1954 (Kimoto et al. 1997; Liao et al. 2014) and so on. However, some early literatures had no detailed information or illustrations on their immature stages. In this paper, some diagnostic characters of the immature stages among several representative species in the genera of Cassidispa, Dactylispa, Dicladispa and Platypria are compared and summarized in Table 1.

Table 1.

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Comparisons of diagnostic characters of immature stages among C. relicta and some species in the genera of Dactylispa, Dicladispa, and Platypria.

Diagnostic characters	Larva			Pupa			References
Diagnostic characters	Lateral scoli on meso- and metathorax	Shape of abdominal scoli	Lateral branches of abdominal scoli	Processes on pronotum	Processes on abdominal apex	Spiracles of fifth abdominal segment	References
C. relicta	absent	rounded	segments VI–VII	absent	2 flattened	short, thick	This paper
D. setifera	absent	triangular	absent	absent	2 flattened	short, pointed	Chen et al. 1986
D. rufiventris	absent	diminutive	absent	absent	2 spinulose	short, pointed	Maulik 1932
D. chapuisi	absent	rounded	absent	absent	2 spinulose	spiniform	Maulik 1932
D. javaensis	present	triangular	absent	absent	2 small	long conical	Maulik 1931
D. higoniae	present	triangular	absent	absent	2 small triangular	long conical	Yano 1965; Kimoto et al. 1997 (larva); Lee and Cheng 2007
D. insulicola	present	triangular	absent	absent	2 flattened	long conical	Lee and Cheng 2007
D. latipennis	present	triangular	absent	absent	2 flattened	long conical	Lee and Cheng 2010
D. doriae	present	triangular	absent	absent	2 pointed	upward-hooked	Zaitsev 2012
D. xanthopus	present	triangular	absent	absent	2 short	upward-hooked	Zaitsev 2012
D. haturanga	present	triangular	segments VI–VII	absent	2 branched	long conical	Zaitsev 2012
Di. armigera	absent	triangular	absent	present	6 small spinous	long pointed	Chen et al. 1986; Kimoto et al. 1997 (larva); Lee and Cheng 2010
Di. testacea	absent	short, fine	absent	absent	2 flattened	elongate-horned	Świętojańska et al. 2014
P. andrewesi	present	triangular	absent	present	2 flattened	long conical	Uhmann 1957 (pupa); Kimoto et al. 1997 (larva)
P. melli	present	triangular	absent	present	2 flattened	long conical	Kimoto et al. 1997 (larva); Liao et al. 2014

The larva of C. relicta is very similar to that of some Dactylispa species and can be distinguished by the lateral branches of abdominal scoli on segments VI–VII (very small and with a rounded apex), although this character also presented on D. haturanga but distinctly slender and with a pointed apex (Zaitsev 2012). Additionally, the abdominal processes are distinctly round in shape (Figs 6–7, 16–17), with a pair of spiracles whose opening directed backward (in genus Dactylispa the spiracles usually formed very pointed or triangular respiratory horns). The pupa of C. relicta is most similar to that of Di. testacea. These two species each have a pair of lateral scoli on abdominal segments I–V and have 2–3 pairs of lateral scoli on segments VI–VIII, the abdominal apex has two flattened processes; and the spiracles of the fifth abdominal segment look very similar. The differences are that the body shape of C. relicta is broadly ovate and the anterior margin of the head is slightly straight, but the head of Di. testacea is distinctly emarginate.

Figures 16–19.

Cassidispa relicta. 16 Larva, dorsal view 17 Larva, ventral view 18 Pupa, dorsal view 19 Pupa, ventral view.

The larvae, especially younger larvae, usually returned to the resting place (Figure 29). Such an interesting and specific behavior was also found in some species of the genus Dactylispa, such as D. subquadrata (Baly, 1874) and D. higoniae (Lewis, 1896) (Yano 1965), but not in D. angulosa (Solsky, 1871) (Yano 1965) and D. approximata Gressitt, 1939 (Dai et al. 2012). The leaf areas with obviously deeper color smeared with larval feces which may help the larvae to avoid predators and parasitoids (Hering 1951; Yano 1965).

Figures 20–21.

Cassidispa relicta, pupa. 20 Dorsal view 21 Ventral view.

Figures 22–27.

Life stages of Cassidispa relicta on host plants (all on Betula platyphylla except Fig. 26 on Ulmus pumila). 22–23 Adults and their feeding pattern 24 Adults copulate on upper surface of host leaf 25–26 Females laying eggs on lower surface of host leaves 27 Female laying eggs on the tree trunk.

Figures 28–34.

Life stages of Cassidispa relicta on host plants Betula platyphylla. 28 Eggs 29 A leaf with new mines of hatching larvae and larval resting places focusing on their egg points: r – resting place of larva 30 Shared large mine of three older larvae in a same leaf 31–32 Mature larva leaves its original mine and builds a new one for overwintering and pupation, in Fig. 32 the red arrow indicates the location of mature larva 33 Pupae in the leaf litter in spring 34 A pupal mine in the fallen leaf.

Acknowledgements

We thank every member of Leafminer Group of Gannan Normal University and Ulanqab Forestry Pest Control and Quarantine Station for collection, laboratorial rearing, observations, and recording of leaf-mining hispines. We also acknowledge the great comments from the three reviewers (Drs. Jolanta Świętojańska, Jesus Gomez-Zurita and Lukáš Sekerka), which soundly improve our first manuscript. Special thanks to Dr. Lukáš Sekerka for correct identification of the species. This study was funded by the National Natural Science Foundation of China (31760173, 41361009, 31260116), Natural Science Foundation of Jiangxi, China (20171BAB204023) and Innovation Team Project of Gannan Normal University.

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