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Seven species of Keilbachia Mohrig are recognized, and among them, two new species, Keilbachia subacumina Wu & Zhang, sp. n. and Keilbachia fengyangensis Wu & Zhang, sp. n. are described and illustrated. Five species, Keilbachia orthonema, Keilbachia flagrispina, Keilbachia demssia, Keilbachia oligonema and Keilbachia acumina are reported for the first time from China. A key to the 15 Chinese species of this genus is also provided.
Diptera, Sciaridae, Keilbachia, new species, Mainland China, Oriental Region
The genus Keilbachia was firstly proposed for Keilbachia nepalensis Mohrig from Nepal (
In this study, seven species of Keilbachia are recognized from Mainland China. Among them, two new species, Keilbachia fengyangensis and Keilbachia subacumina are described and illustrated. Five species, Keilbachia flagrispina, Keilbachia demssia, Keilbachia orthonema, Keilbachia oligonema and Keilbachia acumina are reported for the first time from China. An additional 8 species of Keilbachia are known from Taiwan China: Keilbachia adjuncta
All specimens were collected by sweeping in the field and
preserved in 75% ethanol. They were mounted on glass slides in
xylol-based Canada balsam after clearing in creosote. The heads of
specimens from Yunnan province were bleached in 10% NaOH for about 24
hours at room temperature. The specimens were observed and measured
under a Nikon SMZ1500 stereoscopic microscope. The illustrations were
prepared under a Nikon Eclipse 50i optical microscope, with an attached
drawing tube. The terminology follows
1. | Gonostylus with subapical megasetae | 3 |
– | Gonostylus with no subapical megasetae | 2 |
2. | Gonostylus with one mesial megaseta | Keilbachia praedicata |
– | Gonostylus with two mesial megasetae | Keilbachia oligonema |
3. | Gonostylus with one or two subapical megasetae | 5 |
– | Gonostylus with three or more subapical megasetae | 4 |
4. | Gonostylus with one mesial megaseta on middle and another one at the base of gonostylus | Keilbachia profana |
– | Gonostylus with only one mesial megaseta at the base of gonostylus | Keilbachia ulcerate |
5. | Gonostylus with two subapical megasetae | Keilbachia adjuncta |
– | Gonostylus with two subapical megasetae | 6 |
6. | Mesial megaseta of gonostylus short, shorter than maximal width of gonostylus | Keilbachia orthonema |
– | Mesial megaseta of gonostylus long, at least as long as maximal width of gonostylus | 7 |
7. | Subapical megasetae of gonostylus close to each other, both at apical fourth of gonostylus | 11 |
– | Subapical megasetae of gonostylus widely apart, basal most one at apical third or apical half of gonostylus | 8 |
8. | Tegmen modified, slightly broader subbasally than subapically | Keilbachia sasakawai |
– | Tegmen simple, much broader subbasally than subapically | 9 |
9. | Basal body of mesial megaseta of gonostylus long and slender | Keilbachia subferrata |
– | Basal body of mesial megaseta of gonostylus short and stout | 10 |
10. | Basalmost subapical megasetae at apical half of gonostylus (Fig. 5) | Keilbachia subacumina |
– | Basalmost subapical megasetae at apical third of gonostylus (Fig. 6) | Keilbachia acumina |
11. | Subapical megasetae of gonostylus subequal in size | 13 |
– | Subapical megasetae of gonostylus not equal in size | 12 |
12. | Apicalmost subapical megaseta of gonostylus slender | Keilbachia ferrata |
– | Apicalmost subapical megaseta of gonostylus stout | Keilbachia grandiosa |
13. | Apex of gonostylus rounded and broad | Keilbachia flagrispina |
– | Apex of gonostylus pointed and | 14 |
14. | Mesial megaseta of gonostylus long and strongly curved (Fig. 10) | Keilbachia fengyangensis |
– | Mesial megaseta short and slightly curved | Keilbachia demissa |
(Male). Body length 1.64–1.71mm; wing length 1.36–1.41mm.
Eye bridge 3–4 facets wide. Length/width of 4th flagellomere 2.19–2.32.
Anterior pronotum with 5–6 setae. Episternum 1 with 3–4 setae.
c/w 0.69–0.72, R1/R 0.71–0.76, r-m with one seta.
The mesial megaseta on gonostylus very long and curved, nearly three times as long as the width of gonostylus. The basal body of mesial megaseta is not distinct. Tegmen simple, much broader subbasally than subapically.
China, Yunnan, Baoshan, Mts. Gaoligongshan, 24°49.729'N; 98°46.074'E, sweep-net 11.V.2009. 4 males, Man-Man Wang [SM00878–00880, SM00882] (ZAFU); 3 males, Su-Jiong, Zhang [SM00886, SM00902–00903] (ZAFU).
China (Yunnan), Myammar, Nepal.
Unknown.
This species is new to China, which was firstly described from Nepal, based on two males. It is similar to Keilbachia ferrata (Hippa & Vilkamaa, 1994) in having two subapical megaseta and a long mesial megaseta, but Keilbachia flagrispina can be separated by the mesial megaseta very long and strongly curved, and two subapical megaseta subequal in length on gonostylus. The materials examined from China do not show distinct variation, but we found the Chinese specimens are much smaller in body length, which is 1.64–1.71 mm, while 2.5 mm in Nepal materials.
(Male). Body length 1.73–1.78 mm; wing length 1.47–1.49 mm.
Eye bridge 3–4 facets wide. Prefrons with 10–12 setae. Length/width of 4th flagellomere 2.17–2.41.
Anterior pronotum with 5–6 setae. Episternum 1 with 7–8 setae.
Length of spur/width of fore tibia 1.20–1.27. Length of metatibia /length of thorax 1.05–1.12.
c/w 0.62–0.64, R1/R 0.52–0.56, r-m with no setae.
The mesial megaseta on gonostylus long and slightly curved, basal body long. Tegmen simple, much broader subbasally than subapically.
China, Yunnan, Baoshan, Mts. Gaoligongshan, 24°49.729'N; 98°46.074'E, sweep-net, 11.V.2009. 6 males, Su-Jiong Zhang [SM00856, SM00859, SM00862, SM00869, SM00875, SM00881] (ZAFU); 1 male, Man-Man Wang [SM00888] (ZAFU).
China (Yunnan), Burma.
Unknown.
This species is new to China, which was firstly described from Burma based on seven males. It is similar to Keilbachia scutica Vilkamaa, Komarova & Hippa, 2006 and Keilbachia flagrispina by the tegmen broadest subbasally (
(Male). Body length 1.76–1.82 mm; wing length 1.35–1.37 mm.
Eye bridge 3–4 facets wide. Prefrons with 9–11 setae. Length/width of 4th flagellomere 2.47–2.53.
Anterior pronotum with 4–5 setae. Episternum 1 with 5–6 setae.
Length of spur/width of protibia 1.76–1.81. c/w 0.79–0.82, R1/R 0.71–0.73, r-m with 0–1 seta.
The mesial megaseta on gonostylus short and straight, slightly longer than its basal body. Two slender megasetae at apical forth of gonostylus. Tegmen slightly broader subbasally than subapically.
China, Yunnan, Yingjiang, Tongbiguan, 24°36.004'N; 97°39.139'E, sweep-net, 20.V.2009. 6 males, Su-Jiong Zhang [SM00657–00658, SM00663–00664, SM00670, SM00680] (ZAFU); 3 males, Man-Man Wang [SM00653, SM00666–00667] (ZAFU).
China (Yunnan), Malaysia.
Unknown.
This species is new to China, which was
firstly described from Sabah, Malaysia, based on two male specimens.
It is similar to Keilbachia apprima Vilkamaa, Komarova & Hippa, 2006 from Vietnam by sharing a short mesial megaseta (
(Male). Body length 1.51–1.57 mm; wing length 1.25–1.28 mm.
Eye bridge three facets wide. Prefrons with 3–5 setae. Length/width of 4th flagellomere 2.35–2.71.
Anterior pronotum with 3–4 setae. Episternum 1 with 4–5 setae.
Length of spur/width of protibia 1.55–1.57.
c/w 0.64–0.65, R1/R 0.72–0.75, r-m with no setae.
Gonostylus with two megasetae widely apart, one at subapical and stout, the other at apical third and slender (Fig. 6). Basal third of gonostylus excavated, with a long and strongly curved subbasal mesial megaseta on broad basal body. Tegmen simple, much broader subbasally than subapically, with sparsely placed teeth.
1 male, China, Zhejiang, Linan, Mt. Xijingshan, 30°23'N; 119°72'E, sweep-net, 21.VI.2008, Su-Jiong Zhang [SM00018] (ZAFU); 1 male, China, Zhejiang, Lishui, Mt. Jiulongshan, 28°59'N; 119°25'E, sweep-net, 10.X.2008, Su-Jiong Zhang [SM00114] (ZAFU); 1 male, Yunnan, Tengchong, Shaba, Mt. Tiantaishan, 25°24.524'N; 98°42.735'E, sweep-net, 13.V.2009, Su-Jiong Zhang [SM00933] (ZAFU).
China (Zhejiang, Yunnan), Japan.
Unknown.
The species is similar to Keilbachia subferrata Rudzinski and Keilbachia ferrata
(Hippa & Vilkamaa) by having a rather long subbasal mesial megaseta
on a large basal body, But it can be distinguished from Keilbachia subferrata by the smaller mesial megaseta in a more apical position (
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Figs 1–5(Male). Body length 1.81–2.32 mm; wing length 1.49–1.52 mm.
Color. Head, thorax and abdomen brown; antenna, palpus, coxae, and hypopygium yellowish-brown; leg yellow; wing fumose.
Head (Figs 1, 2). Eye bridge 3–4 facets wide. Prefrons with 5–6 setae, clypeus with 0–1 seta. Palpus three-segmented. Basal segment with one seta, with a narrow sensory pit, 2nd segment with 4–6 setae, 3rd segment with 6–7 setae. Length/width of 4th flagellomere 2.74–2.79.
Thorax. Anterior pronotum with 5–6 setae. Episternum 1 with 6–7 setae.
Legs. Apex of protibia (Fig. 3). Length of spur/width of protibia 1.79–1.83. Length of profemur/length of protibia 0.76–0.79. Length of metatibia /length of thorax 1.23–1.31.
Wings.Width/length 0.44–0.49. c/w 0.71–0.77, R1/R 0.96–0.98. r-m with 1–2 setae.
Abdomen. Sternite 8 with 10–11 setae. Gonostylus and gonocoxa subequal in length. Gonostylus with two megasetae widely apart, one at apex and stout, the other at apical half of gonostylus and slender. Basal third of gonostylus excavated, with a long and strongly curved mesial megaseta on broad basal body. Tegmen simple, much broader subbasally than subapically, with sparsely placed teeth. (Figs 4, 5).
1–5, Keilbachia subacumina, male. 1 4th flagellomere, lateral view 2 palpus, lateral view 3 apex of protibia, prolateral view 4 part of hypopygium, ventral view 5 gonostylus, ventral view. 6 Keilbachia acumina, male, gonostylus, ventral view. Scale bar = 0.1 mm.
Holotype, male. China, Zhejiang, Linan, Mt. Xijingshan, 30°23'N; 119°72'E, sweep-net, 21.VI.2008, Su-Jiong Zhang [SM00025] (ZAFU). Paratypes. 2 males, same data as holotype [SM00018, SM00024] (ZAFU); 5 males, same data as holotype but 19.VII.2008 [SM00057–00061] (ZAFU). China, Zhejiang, Lishui, Mt. Fengyangshan, 28°04'N; 119°08'E. sweep-net, 3 males, 11.VIII.2008, Sheng-Long Liu [SM00282] (ZAFU); 1 male, 24.VIII.2008, Sheng-Long Liu [SM00231–00233] (ZAFU); 1 male, 01.VIII.2008, Xiao-Ling Niu [SM00306] (ZAFU). 1 male, China, Zhejiang, Lishui, Mt. Jiulongshan, 28°59'N; 119°25'E, sweep-net, 10.X.2008, Su-Jiong Zhang, [SM00114] (ZAFU).
Unknown.
This species is very similar to Keilbachia acumina in the structure of the hypopygium (Fig. 5, 6), but Keilbachia subacumina can be distinguished by having the apex of gonostylus broader, and the stouter and shorter basalmost megaseta at the apical half of the gonostylus. What’s more, the anterior pronotum bears 5–6 setaein Keilbachia subacumina while 3–4 setae in Keilbachia acumina, and the r-m nervation of the wing with 1–2 setae in Keilbachia subacumina while bare in Keilbachia acumina. The structure of the hypopygium in the new species does not show distinct intraspecies variation. The species is named after its similarity to Keilbachia acumina. This epithet is an adjective.
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Figs 7–11(Male). Body length 2.31–2.48 mm; wing length 1.92–1.94 mm.
Color. Head, thorax and abdomen brown; antenna, palpus, coxae, legs and hypopygium yellowish-brown; wing fumose.
Head (Fig. 7, 8). Eye bridge 3–4 facets wide. Prefrons with 7–8 setae, clypeus with no setae. Palpus three-segmented. Basal segment with one seta, with wide sensory pit, 2nd segment with 4–6 setae, 3rd segment with 7–8 setae. Length/width of 4th flagellomere 2.91–2.94.
Thorax. Anterior pronotum with 4 setae, episternum 1 with 5–6 setae.
Legs. Apex of protibia (Fig. 9). Length of spur/width of protibia 1.45–1.49. Length of profemur/length of protibia 0.65–0.68. Length of metatibia/length of thorax 1.03–1.11.
Wings. Width/length 0.45–0.47. c/w 0.62–0.69; R1/R 0.83–0.91. r-m with one seta.
Abdomen. Sternite 8 with nine setae. Gonostylus longer than gonocoxa, slightly curved, with two slender apical and subapical megaseta. The basal third of gonostylus with a long and curved mesial megaseta on a narrow and short basal body. Tegmen higher than broad, with sparse placed teeth (Fig. 10, 11).
Keilbachia fengyangensis, male. 7 4th flagellomere, lateral view 8 palpus, lateral view 9 apex of protibia, prolateral view 10 gonostylus, ventral view 11 part of hypopygium, ventral view. Scale bar = 0.1 mm.
Holotype, male. China, Zhejiang, Lishui, Mt. Fengyangshan, 28°04'N; 119°08'E, sweep-net, 26.IV.2008, Sheng-Long Liu [SM00342] (ZAFU). Paratypes. 2 males, same data as holotype [SM00335, SM00338] (ZAFU); 1 male, same data as holotype but 19.IV.2008 [SM00346] (ZAFU).
Unknown.
This species is found only from Mt. Fengyangshan, Zhejiang. It is similar to Keilbachia demissa and Keilbachia flagrispina by having a long mesial megaseta, but it can be distinguished from Keilbachia demissa by having the megaseta of the gonostylus longer and more curved, and the tegmen with no distinct basolateral. The new species can be distinguished from Keilbachia flagrispina by the apex of gonostylus distinct attenuated, and the mesial megaseta shorter and not strongly curved. The species is named after its type locality (Mt. Fengyangshan).
(Male). Body length 1.71 mm; wing length 1.66 mm.
Eye bridge four facets wide. Prefrons with nine setae. Length/width of 4th flagellomere 3.30.
Anterior pronotum with five setae, episternum 1 with three setae.
Apex of protibia. Length of spur/width of protibia 1.48.
Width/length 0.40, c/w 0.73, R1/R 0.87. r-m with no setae.
Gonocoxa ventrally with a slight indication of an intercoxal lobe. Gonostylus nearly as long as gonocoxa, with no apical and subapical megaseta. The basal third of gonostylus excavated, with two long and curved mesial megaseta on a broad basal body. Tegmen simple, much broader subbasally than subapically.
1 male. China, Yunnan, Tengchong, Dahaoping, 24°55'N; 98°45'E, sweep-net, 22.V.2009, Man-Man Wang, [SM00757] (ZAFU).
China (Yunnan), Burma.
Unknown.
The gonostylus of Keilbachia oligonema
Hippa & Vilkamaa, 2007 with two long and curved mesial megaseta,
and without apical and subapical megaseta. It is different from all the
other species in the group of flagria, which has more than one mesial megaseta on the gonostylus. Keilbachia oligonema
is unique in having different characters in width of the eye bridge,
setosity of sternite 8, and the ventral intercoxal area of the
hypopygium between the holotype and two additional specimens, from
which the authors suspected they may represent two different species (
We wish to express our sincere thanks to Dr. Heikki Hippa (Swedish Museum of Natural History, Stockholm), Dr. Pekka Vilkamaa (Finnish Museum of Natural History, Helsinki), Dr. Frank Menzel (Senckenberg Deutsches Entomologisches Institut, Müncheberg) and Dr. Werner Mohrig (Poseritz, Germany) for their kind cooperation and generous help. Special thanks are given to Dr. Hippa for his advice to improve the text. We also thank the collectors of the materials examined, Manman Wang from Zhejiang University and Shenglong Liu from Fengyangshan National Nature Reserve. This study was supported by NSFC grant 30870334.