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Research Article
The leaf-mining genus Antispila Hübner, 1825 feeding on Vitaceae in Shandong Peninsula, China with one new species (Lepidoptera, Heliozelidae)
expand article infoNan Wang, Tengteng Liu, Jiasheng Xu§, Bin Jiang|
‡ Shandong Normal University, Jinan, China
§ Gannan Normal University, Ganzhou, China
| Kunyushan Forest Farm, Yantai, China
¶ Kunyushan National Nature Reverse, Yantai, China
Open Access

Abstract

The Antispila species feeding on Vitaceae from Shandong Peninsula, China are treated. Antispila kunyuensis Liu, sp. n., feeding on Ampelopsis humulifolia, is described as new to science, and A. uenoi Kuroko, 1987, feeding on Vitis amurensis, is recorded as new for China. Vitis amurensis is documented as a new host plant for A. uenoi. The adult male and female, host plant and typical patterns of leaf-mines of both species are illustrated, as are male and female genitalia and venation. The venation and the paired tufts of scales on the 7th abdominal segment in male are illustrated for A. uenoi for the first time. DNA barcodes of both species are also provided, together with a neighbor-joining tree for facilitating species delimitation.

Keywords

Ampelopsis, Antispila, China, DNA barcode, Heliozelidae, Lepidoptera, new species, Shandong Peninsula, Vitis

Introduction

The family Heliozelidae comprises 126 described species in 12 genera (van Nieukerken et al. 2011, 2012, van Nieukerken and Geertsema 2015, Liu and Wang 2017), with the largest diversity in North America and Australia. The Heliozelidae were only recently recorded as new for China, with one formally published species (Liu and Wang 2017), but also several database records in BOLD (http://www.boldsystems.org). However, the knowledge of Chinese Heliozelidae is slowly increasing, and two more species feeding on Vitaceae are described in the present paper. Vitaceae comprises an important group of hosts for Heliozelidae, especially for the genus Antispila Hübner, 1825 (van Nieukerken et al. 2012). Eleven out of the 20 Palearctic and Oriental species of Antispila feed on Vitaceae (Meyrick 1926, Kuroko 1961, 1987, van Nieukerken et al. 2012, van Nieukerken and Geertsema 2015).

Shandong Peninsula is located in the east of China, facing the Korean Peninsula across the Yellow Sea. Although located in a relatively developed region, the arthropod diversity is still rather poorly known and lacks systematic work, so new species, especially small-sized ones, can still be discovered. Here, we discovered two species of the leaf-mining genus Antispila during an ongoing biodiversity exploration in Shandong Peninsula. One of these is a new species, the other one, A. uenoi Kuroko, 1987 is new for China. Both are described here in detail, increasing the number of known species of the genus Antispila in China to three.

Material and methods

Leaves with active mines were placed in small plastic bags for rearing. After the shields had been exscinded and the larvae had left the mines, leaves with vacant leaf-mines were dried in a plant press. The larval shields, the corresponding adults, and the vacant leaf-mines were identically coded.

Genitalia and wings were dissected and mounted according to the methods introduced by Li (2002), but stained with Eosin Y and/or Chlorazol Black. Illustrations were prepared by using a Leica DM1000 microscope. Adult photographs were taken with a Leica S6D stereo microscope. Photographs of the host plants and leaf-mines were taken in the field using a Canon EOS 650D camera plus a Macro Lens, and enlarged photographs of leaf-mines were taken with the Leica S6D stereo microscope.

DNA was extracted from adult specimens preserved in 95% ethanol in Shandong Normal University, Jinan, China, with the whole body skeleton including genitalia and wings preserved as vouchers (Knölke et al. 2005). Protocols for total DNA extraction and mitochondrial COI gene amplification followed that described in our previous study (Liu and Wang 2017). The sequence data used in this study have been deposited in GenBank and in the BOLD database, a public dataset “DS-ANTIVIT” (https://doi.org/10.5883/DS-ANTIVIT). Sequences were aligned using the MUSCLE model and genetic distance estimation was analyzed using the Kimura 2-Parameter model in BOLD.

Terminology for adults follows van Nieukerken et al. (2012); the term canalis spiralis of female genitalia follows Kuroko (1987). The classification of the host plants is based on APG (2016), and plant scientific names follow The Plant List (2013).

All the specimens examined, including the holotype of the new species, are deposited in the Zoological Collection of Shandong Normal University (SDNU). The type depository of A. uenoi Kuroko, 1987, collection of the Entomological Laboratory, University of Osaka Prefecture, is abbreviated as UOP.

Taxonomy

Antispila kunyuensis Liu, sp. n.

Figs 1, 2, 6, 9, 11–15, 26–29, 34–38

Type material

Holotype. ♂, China, Shandong Province, Yantai, Mt. Kunyu National Nature Reverse, 121.740°E, 37.292°N, 400 m, larva coll. 2017.vi.28, mine on Ampelopsis humulifolia, emerged viii.06, collector Bin Jiang, genitalia no. SDNU.LIU0014, registered no. SDNU.YT170601.2. Paratypes. 3♂, 3♀, genitalia nos. SDNU.LIU0009♂, SDNU.LIU0016♀, SDNU.LIU0044♂, DNA voucher slide no. SDNU.LIU0013♀ (whole body on one slide), registered nos. SDNU.YT170601.1, SDNU.YT170601.3–6, other data same as holotype.

Other material

Leaf-mine. Shandong Province: Yantai, Mt. Kunyu National Nature Reverse, 121.740°E, 37.292°N, 400 m, vacant mine coll. 2016.vii.31, on Ampelopsis humulifolia, collector Tengteng Liu & Encui Wang, registered no. SDNU.YT160761–7, YT160785.

Diagnosis

Two Antispila species, A. ampelopsia Kuroko, 1961 and A. orbiculella Kuroko, 1961, are known to feed on Ampelopsis, and both associate with the same species, A. brevipedunculata. Antispila kunyuensis can easily be distinguished from A. ampelopsia by the fine features of the phallus and the ovipositor, and from A. orbiculella by the two separate basal spots that are joined forming a transverse fascia in the forewing.

Description

Adult (Figs 1, 2, 6). Forewing length 1.7–2.1 mm. Head silvery gray, with reddish reflection, more apparent on front. Antennae dark fuscous, silvery gray on distal two segments. Labial palpus silvery gray, pointed apically. Thorax and tegula dark fuscous. Legs gray, with blackish gray pigmentation outer surface. Forewing dark fuscous, with strong reddish reflection; two pairs of opposite triangular silvery spots on costa and dorsum, the inner pair with costal spot before middle, dorsal spot at basal 1/4, the outer pair with costal spot at 3/4, dorsal spot largest, near tornus; cilia unicolorous with forewing on basal 3/4, whitish gray on distal 1/4. Hind wing dark gray, cilia darker. Abdomen dark gray dorsally, yellowish gray ventrally.

Figures 1–8. 

Adult of Antispila species. 1 A. kunyuensis, holotype, male, SDNU.QD170705.1 2 A. kunyuensis sp. n., paratype, female, SDNU.YT170601.4 3 A. uenoi, male, SDNU.QD170705.1 4 A. uenoi, female, SDNU.QD170705.3 5 A. uenoi, female, SDNU.QD170707 6 A. kunyuensis, male, paratype, ventral view of forewing indicating bristles of retinaculum, SDNU.YT170601.3 7 A. uenoi, ventral view of forewing indicating bristles of retinaculum 8 A. uenoi, male, indicating paired tufts of slender scales on the 7th abdominal segment, photographed during dissection in water, SDNU.QD170705.1. Scale bars: 1.0 mm.

Venation (Fig. 9). Forewing with Sc reaching before middle on costa; R1 from 2/5 on upper margin of cell to costal 3/5, Rs1 from distal 1/7 on upper margin of cell to costal 3/4, Rs2 from beyond distal end of cell, Rs3+4 reaching costa before apex; cell triangular distally; M1 stalked with Rs3+4, to termen near apex, M2+3 from near distal end of cell; CuA from distal 1/6 of lower margin of cell; A1+2 to beyond middle of dorsum. Hindwing with Sc to costal 3/5, R+M ending in 3 branches: Rs to costa near apex, M2 and M3 to dorsum; Cu to middle of dorsum; A1+2 weak. Male with one long frenulum, female bearing two shorter frenular bristles.

Figures 9–10. 

Wing venation of Antispila species. 9 A. kunyuensis, paratype, female, SDNU.LIU0013 10 A. uenoi, male, SDNU.LIU0012.

Male genitalia (Figs 11–15). Tuba analis developed (Fig. 12). Uncus bar-shaped, protruded towards posteriorly at middle. Vinculum shorter than phallus, slightly rectangular on anterior margin. Valva more or less triangular, digital process long and narrow, almost same length as valva, pecten on pedicel, with nine comb teeth (Fig. 13). Juxta half as long as phallus, densely covered with small teeth on basal 2/5, anterior arrow pointed on basal corners. Phallus nearly as long as length of vinculum + tegumen, narrowed anteriorly (Fig. 11); phallotheca with five to six strong teeth and a group of smaller sharp-pointed teeth, with a cluster of smaller spines at base of juxta; distal part with a mushroom-shaped process and a straight process ventrally (Figs 14, 15).

Figures 11–15. 

Male genitalia of Antispila kunyuensis. 11 whole genitalia, SDNU.LIU0014 12 tegumen, SDNU.LIU0009 13 valva, same slide as 12 14 phallus, lateral view, SDNU.LIU0044 15 enlarged distal part of phallus, same slide as 14.

Female genitalia (Figs 26–29). Ovipositor with three cusps at either side, with basal one largest and middle one smallest (Fig. 28). Vestibulum round, more or less sclerotized (Fig. 29). Corpus bursae membranous.

DNA barcode

One DNA barcode from a paratype was obtained. A neighbor-joining tree, covering most Asian Antispila species and other Vitaceae-feeding species, was generated for facilitating species delimitation (Fig. 33).

Host plant

Ampelopsis humulifolia Bunge (Figs 34, 35). The placement of the leaf-mines is variable (Figs 36–38), from the base to the apex of a leaf, from absolutely along veins to between but not touching veins. This results in variably-shaped blotch mines, but the majority of blotches are more or less round (Figs 38, 42–45). Frass primarily occupies the opposite side of the cut-out in round blotch mines, or occasionally disperses throughout the mine (Fig. 37), but always in a broad medial band in wide gallery mines (Fig. 38). This species overwinters as a prepupa in the shield. A single generation per year was observed at the type locality.

Distribution

China (Shandong).

Etymology

The specific name is derived from the type locality of the new species, Mt. Kunyu, representing the most famous nature reserve in Shandong Peninsula, focusing on forest ecosystem conservation.

Antispila uenoi Kuroko, 1987

Figs 3–5, 7, 8, 10, 16–18, 19–25, 30–31, 39–41, 46–49

Antispila uenoi Kuroko, 1987: 113. TL: Japan (Iwate Prefecture). TD: UOP.

Material examined

China: 2♂, 3♀, Mt. Laoshan, Qingdao, Shandong Province, 120.609°E, 36.204°N, 400 m, larva coll. 2017.vii.01, mine on leaf serration of Vitis amurensis, case made vii.03, emerged vii.15, collectors Tengteng Liu and Nan Wang, genitalia no. SDNU.LIU0008♀, SDNU.LIU0015♂, DNA voucher slide no. SDNU.LIU0011♂ (whole body on one slide), registered no. SDNU.QD170705.1–3; 2♂, 2♀, Mt. Laoshan, Qingdao, Shandong Province, 120.609°E, 36.204°N, 400 m, larva coll. 2017.vii.01, mine on leaf basal area, case made vii.03, emerged vii.15, genitalia no. SDNU.LIU0043♀, DNA voucher slide no. SDNU.LIU0012♂ (whole body on one slide), collectors Tengteng Liu and Zhenquan Gao, registered nos. SDNU.QD170707, SDNU.QD1707.1–2.

Diagnosis

Kuroko (1987) gave a detailed diagnosis to distinguish A. uenoi from A. ampelopsia.

Description

Adult (Figs 3–5, 7). Forewing length 1.6–1.8 mm. Head silvery gray, with reddish and purple reflection. Antennae dark fuscous, silvery on distal two segments. Labial palpus silvery gray, pointed apically. Thorax and tegula dark fuscous. Legs black, with whitish gray pigmentation on distal part of tarsomeres. Forewing blackish fuscous, with strong purple reflection; an oblique silvery fascia from before middle on costa to basal 1/4 on dorsum, a triangular silvery spot on costal 3/4, with a similar one opposite to it near tornus; cilia unicolorous with forewing on basal 3/4, whitish gray on distal 1/4. Hind wing gray, cilia darker. Abdomen dark gray dorsally, gray ventrally.

Female with forewing patterns more distinct (Figs 4, 5).

Venation (Fig. 10). Forewing with Sc reaching before middle on costa; R1 from 2/5 on upper margin of cell to costal 3/5, Rs1 from distal 1/7 on upper margin of cell to costal 3/4, Rs2 from well beyond distal end of cell, Rs3+4 reaching costa before apex; cell truncated distally; M1 stalked with Rs3+4, to termen near apex, M2+3 from lower corner of distal end of cell; CuA from distal 1/7 of lower margin of cell; A1+2 to beyond middle of dorsum. Hindwing with Sc to beyond middle of costa, R+M ending in 4 branches: Rs to costa, M1 to dorsum near apex, M2 and M3 to dorsum; Cu to middle of dorsum; A1+2 weak. Male with one long frenulum, female bearing two shorter frenular bristles.

Male genitalia (Figs 1625). Tuba analis developed. Uncus bar-shaped, with two papillae bearing two short setae each at middle, bearing one long and a few shorter setae laterally (Fig. 22). Vinculum shorter than phallus, rounded on anterior margin. Valva semicircular on ventral margin, digital process about half the width of valva, pecten with 12 comb teeth (Figs 20, 21). Juxta longer than half length of phallus, anterior arrow broad and almost semicircular. Phallus as long as length of vinculum + tegumen, narrowed anteriorly; phallotheca with groups of spines, more concentrated and larger ventrally (Figs 24, 25); distal part with two processes ventrally, one large and curved, the other V-shaped with one branch larger than the other, two smaller similar processes dorso-apically, one less sclerotized and curved process at apex with several membranous teeth ventrally (Fig. 23). Paired tufts of slender scales on the 7th abdominal segment (Figs 8, 17, 18).

Figures 16–18. 

Male genitalia and abdomen of Antispila uenoi. 16 whole male genitalia, unrolled, SDNU.LIU0015 17 abdomen, ventral view, notice the tuft of slender scales on the 7th segment, left tuft detached, same slide as 16 18 enlarged view of tuft of slender scales, same slide as 16.

Figures 19–25. 

Male genitalia of Antispila uenoi. 19–23 SDNU.LIU0011 19 tegumen and vinculum, 20 valva 21 enlarged view of comb 22 tegumen 23 phallus, lateral view 24 enlarged view of spines on base of juxta, SDNU.LIU0012 25 same part as 24 under a shallow focus.

Figures 26–29. 

Female genitalia of Antispila kunyuensis. 26 female genitalia, SDNU.LIU0016 27 female genitalia, SDNU.LIU0013 28 detail of ovipositor tip, same slide as 26 29 detail of vestibulum, same slide as 26.

Female genitalia (Figs 30–32). Ovipositor with six cusps at either side, with apical three smaller, tip distinctly indented (Fig. 32). Vestibulum membranous, with a sclerotized granule and a weak circular ring surrounding opening of canalis spiralis (Fig. 30). Corpus bursae membranous.

Figures 30–32. 

Female genitalia of Antispila uenoi. 30 female genitalia, SDNU.LIU0043 31 posterior apophyses and ovipositor tip, same slide as 30 32 detail of ovipositor tip, same slide as 30.

DNA barcode

Two DNA barcodes were obtained (Fig. 33). A partial DNA barcode of 268 bp generated from a paratype of A. uenoi (RMNH.INS.24531) was used for identification of the Chinese specimens. The genetic distance between the Chinese specimens and the paratype is 1.53%.

Figure 33. 

Neighbor Joining Tree, based on DNA barcodes of Antispila species, especially Vitaceae-feeding and Asian species.

Host plants

Vitis amurensis Rupr. (Fig. 39), V. coignetiae Pulliat ex Planch. and V. labruscana L.H. Bailey (Kuroko 1987). Vitis amurensis is recorded here as a new host.

Biology

Leaf-mines on Vitis amurensis can occupy serrations along the leaf margin (Figs 40, 41, 46–48) or the leaf basal area (Fig. 49) in an almost equal proportion, calculated from our rearing data (5 : 4); no other placements (e. g. leaf central area) of mines were observed, although in Japan the majority of mines occupy the apical or marginal area of the leaves on other hosts (Kuroko 1987). Frass often dispersed along mines (Figs 47–49). This species overwinters as a prepupa in the shield. Two generations probably occur in Shandong Peninsula.

Figures 34–41. 

Host plant and leaf-mines of Antispila species. 34–38 A. kunyuensis 34 Host plant, Ampelopsis humulifolia 35 unripe fruits of A. humulifolia 36–38 leaf-mines 39–41 A. uenoi 39 Host plant, Vitis amurensis 40 leaf-mines along leaf margin, indicated by red arrows 41 leaf-mine with a dead larva.

Figures 42–49. 

Leaf-mines of Antispila species. 42–45 A. kunyuensis, fig. 42 is the identical mine to Fig. 37, one dead larva and a parasitoid, most likely a species of Eulophidae in Figure 45 46–49 A. uenoi, leaf-mines occupying leaf serration and apical area in Figures 46–48, leaf-mine occupying leaf basal area in Figure 49.

Distribution

China (Shandong), Japan: Honshu. The host plant Vitis amurensis is widespread in the northeast and eastern parts of China (Chen et al. 2007), Eastern Russia (Afonin et al. 2008), Japan: Honshu and Korea (Ohwi 1965). A much wider distribution of the moth is expected, where its host plants occur.

Remarks

This species is newly recorded in China. The venation and the paired tufts of scales on the 7th abdominal segment in male are illustrated for A. uenoi for the first time.

Discussion

In the Miocene, the arthropod diversity of Shandong Peninsula was quite rich, which is well documented by numerous fossil records from Shanwang, Shandong province (Zhang 1989, 1994). During the last centuries, the Yellow River shifted its mouth a number of times and finally diverted to the Bohai Sea in 1855 (Cheng and Xue 1997, Wang et al. 2010). Both long-term historical and recent regional processes may have significantly influenced the species richness of Shandong Peninsula, which makes this peninsula an interesting region for biogeographical and biodiversity studies (e.g., Zheng et al. 2009). Heliozelidae, together with other small-sized Lepidoptera species, are expected to experience an increase in species richness upon a deeper exploration of this region, as will be the case for all of China. For instance, in Argyresthiidae, the number of species increased from 14 to 64 after the study by Liu et al. (2017). This would be true when considering the host preference of the heliozelids and the rich diversity of Vitaceae and many other host families in China. Take Vitaceae for example. A majority of Antispila species show a specific host preferences for Vitaceae and Cornaceae (Milla et al. 2017, van Nieukerken et al. 2018), and there are 146 species of Vitaceae distributed in eight genera in China, with 87 endemic species, which are mostly native to central, south, and southwest China (Chen et al. 2007).

Acknowledgements

We thank the staff of the Kunyu National Nature Reserve for their support during the field work. We thank reviewers Toshiya Hirowatari and Erik J. van Nieukerken for their insightful comments and corrections which greatly improved the manuscript. This study is supported by the Shandong Provincial Natural Science Foundation, China (No. ZR2017BC051).

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