Research Article |
Corresponding author: Nestor Fernandez ( nestorfernand51@yahoo.fr ) Academic editor: Vladimir Pesic
© 2017 Nestor Fernandez, Pieter Theron, Sergio Leiva, Louwrens Tiedt.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Fernandez N, Theron P, Leiva S, Tiedt L (2017) Revision of the family Carabodidae (Acari, Oribatida) XII. Yoshiobodes camerunensis sp. n. and Rugocepheus costaricensis sp. n. ZooKeys 706: 31-50. https://doi.org/10.3897/zookeys.706.14807
|
Yoshiobodes camerunensis sp. n., collected in Cameroon, is the first species of this genus reported from the Afrotropical region. Diagnostic characters include lamellae terminating in a bridge and not in lamellar tips; cup-shaped bothridia, bothridial ring present; rostral setae cochleariform, smooth; lamellar setae slightly lanceolate, barbate; fifteen pairs of notogastral setae; c3 lanceolate, rounded end, with longitudinal shallow grooves; other notogastral setae curved lanceolate-cochleariform. Rugocepheus costaricensis sp. n. is the third species of the genus to be described, and the first collected outside the African region. Prodorsum presents a Y-shaped structure; elevated interlamellar process, superior flat zone; lamellae lacking lamellar tips; fourteen pairs of notogastral setae; four notogastral furrows, and an unpaired elevated central area devoid of setae. Both species are described and illustrated based on adult specimens, studied by means of optical and SEM microscopy.
Carabodidae , Rugocepheus , Yoshiobodes
Extensive collection materials of the family Carabodidae sampled in Africa (Cameroon, Kenya, Zimbabwe, Rwanda, South Africa, Madagascar, Gabon, Comoros, Republic of the Congo, Democratic Republic of the Congo, Nigeria, Ghana); South and Central America (Argentina, Chile, Brazil, Paraguay, Uruguay, Bolivia, Peru, Ecuador, Costa Rica, Martinique, Honduras, Guadeloupe, Trinidad-Tobago), and Asia (Vietnam, China, Cambodia, Sri Lanka) are housed in the Museum national d’Histoire naturelles, Paris (
The taxonomy of the genus Yoshiobodes is complex. This genus comprises 12 species and is divided into three subgenera: Yoshiobodes, which includes eight species with Pantropical (excluding Ethiopic) and Subtropical (Holarctic Southern) distribution; Berndobodes with two species from Borneo, and Dongnaibodes with two species from Vietnam (Subias 2017). According to
This genus is very difficult to study using optical microscopy due to their small size, cuticular microsculpture, cerotegumental layer, particular topography, and setal particularities. The complimentary use of Scanning Electron Microscopy (SEM) is fundamental to understanding and clarifying several aspects of this fascinating group of Carabodidae. The contribution by
The second species, Rugocepheus costaricensis sp. n. is described from Costa Rica. Two species of this genus are known previously from Africa, namely Rugocepheus formosus Mahunka, 2009 and Rugocepheus joffrevillei Fernandez, Theron & Rollard 2013, both from Madagascar.
The techniques used in the light and scanning electron microscopic investigations of the examined specimens follow those proposed by
The SEM observations were made using Scanning Electron Microscope FEI-Quanta Feg 250, with 10 Kv and working distant (WD) variable.
Measurements taken: total length (from tip of rostrum to posterior edge of notogaster); width (widest part of notogaster) in micrometers (μm). Leg setation studies making use of standard, polarized and phase contrast microscopes are provisional, due to the fact that only adult specimens were available for study. Setal formulae of the legs include the number of solenidia (in parentheses); tarsal setal formulae include the famulus (ε).
Morphological terms and abbreviations used are those developed by F. Grandjean (1928–1974) (cf.
The specific epithet is derived from Cameroon, country of origin of the type material.
Holotype. Adult female “CAM 73/3. Mt.Kala (près de Yaoundé). 800–850 m, terreau troncs pourris et litière. IV–V. 1973. Leg. G. TERRON”. Material deposited in the collection of MNHG, Switzerland, preserved in 70% ethanol. Paratypes. 2 adult females “CAM 73/3. Mt. Kala (près de Yaoundé). 800–850 m, terreau troncs pourris et litière. IV–V. 1973. Leg. G. TERRON”. Material deposited in the collection of MNHG, Switzerland, preserved in 70% ethanol.
Setation. Rostral setae cochleariform, smooth; lamellar setae slightly lanceolate, barbate, covered by cerotegumental layer; notogastral c1, c2, da, dm, dp, lm, lp curved lanceolate-cochleriform; c3 lanceolate, rounded end with longitudinal shallow grooves; subcapitular h, epimeral, genital, anal, adanal setae spiniform. Prodorsum. Shallow lamellar furrow present; lamellae terminate in bridge not lamellar tips. Bothridia cup-shaped, with bothridial ring. Barbed fan-shaped sensillus. Notogaster: fifteen pairs of setae. Genital opening on elevated zone; deep anterior furrow in front of genital opening.
Measurements. SEM: length: 301 μm (296–312). Width: 130 μm (127–152) (three specimens). Light microscopy: 311 μm (301–323) × 142 μm (138–148) (two specimens) All specimens female.
Colour: Specimens without cerotegument, light brown, slightly shiny when observed in reflected light.
Cerotegument: Entire body, femora and genua of legs covered by thin layer of between 0.1–0.5 μm presenting as a polygonal network (Figures
Cuticular microsculpture. Prodorsum. Posterior zone of e.i.p and p.p.d round to ovoid depressions (Figure
Yoshiobodes camerunensis sp. n. Adult with cerotegumental layer, SEM. 6 frontal view 7c3 setae 8in setae 9si and sensillar furrow (s.fu) 10lam lateral with le setae 11lam, lateral with le setae 12 rostral setae 13lam dorsolateral with le setae. Scale bars: 50 μm (6); 2 μm (7); 10 μm (8); 10 μm (9); 5 μm (10); 10 μm (11); 5 μm (12); 2 μm (13).
Yoshiobodes camerunensis sp. n. Adult, with cerotegumental layer, SEM. 14 lateral view, zone of humeral apophysis 15 anterior prodorsum region 16 cerotegument and cuticular zone: anterior notogaster 17 notogastral setae, frontal view 18 cuticular microsculpture 19 cuticular depressions 20 frontal view, notogaster. Scale bars: 10 μm (14); 20 μm (15); 10 μm (16); 10 μm (17); 2 μm (18); 2 μm (19); 20 μm (20).
Setation. Seta in lanceolate, barbate, slightly curving (Figure
Prodorsum. Very complex, described from different angles in order to properly interpret the structure. Lateral view (Figure
Yoshiobodes camerunensis sp. n. Adult, with cerotegumental layer, SEM. 21ventral view 22 anal plate, ventral view 23 genital plate, ventral view 24 palp 25 epimeral microsculpture with epimeral setae 26 anal setae 27 subcapitulum. Scale bars: 100 μm (21); 20 μm (22, 23); 10 μm (26); 5 μm (24); 2 μm (25); 10 μm (27).
Frontal view (Figures
Notogaster. Shape: oval in dorsal view; d.sj narrow, well-delimited, curving slightly backwards (Figures
Lateral view: convex (Figure
Lateral region (Figures
Ventral region. Cuticular microsculpture obviously different on epimeral, aggenital, and adanal zones (See Cuticular microsculpture). Subcapitular setae a, m, h (Figures
Epimeres well defined by furrows, easily discernible both in animals with cerotegumental layer (Figure
Yoshiobodes camerunensis sp. n. Adult with cerotegumental layer, SEM. 28 notogaster, posterolateral 29 subcapitulum, lateral 30 cuticular micrsoculpture covered by cerotegumental layer, dorsal zone 31 subcapitular h setae 32 subcapitular setae m33 anal setae 34 discidium and epimeral setae 35 rostral setae, lateral view. Scale bars 20 μm (28); 10 μm (29, 34); 5μm (32, 33); 2 μm (30, 31); 1.5 μm (35).
Legs. Setal formulae (trochanter to tarsus) (Table
Leg | Femur | Genu | Tibia | Tarsus |
---|---|---|---|---|
I | (l). d, dv | (l),v’ | (l),(l) | (pv),s,(a),(u),(p), (tc), (ft),ε,it” |
σ | φ1, φ2 | ω1, ω2 | ||
II | (l), d, dv | (l),d | (l), v’ | (pv), s, (a), (tc), (u), (p), (ft),(it) |
σ | φ | ω ω | ||
III | l,v | d,v,l’ | l’ | (pv), s, (a), (tc), (u), (p), (ft),(it) |
σ | φ | |||
IV | d,ev | d, l’ | (v) | (pv),(u),(p),ft”,s,(a), (tc) |
σ |
The positioning of the le setae during activation of the protection mechanism is interesting: these setae are shielded under the lamellae, but are also further protected by the cerotegumental layer (Fig.
The specific epithet is derived from Costa Rica, country of origin of the type material.
Holotype. Adult female “CCR 0978 Tu 11 Costa Rica Turrialba foret naturelle du catie alt. 560 m. Triage d’humus coté est surface nid d’Atta au pied de Castilla elastica 1.IX. 1978. Leg. P.WERNER” Deposited in the Ccllection of the
Body and legs entirely covered by simple porous cerotegumental layer. Integumental microsculpture over entire body: irregular, small tuberculate. Prodorsum. More or less triangular in dorsal view; deep low lamellar furrow delineating Y-shaped structure; elevated interlamellar process with superior flat zone; CSO present; rostrum beak-shaped; tutorium and Pedotecta I expanded laterally; small triangular discidium; supratutorial depression deep, with several rounded depressions. Lamellae without lamellar tip, forming bridge concealing le setae. Smooth ring-shaped bothridium; bothridial tooth present; sensillus barbate. Fourteen pairs of notogastral setae c1, c2, da, dm, dp, la, lm, lp, h1, h2, h3, p1, p2, p3. Four notogastral furrows present: paired central longitudinal furrow; one lateral unpaired semicircular furrow; another unpaired semicircular furrow delimiting an unpaired elevated central area (devoid of setae); a pair of elevated areas (with c1, da, dm, dp); unpaired semicircular elevated area (with c2, la, lm, lp, h1, h2). Anterior genital furrow clearly observed; epimere 4 borders elevated; genital plate situated in elevated zone surrounded by furrow. Epimeral chaetotaxy 3-1-3-3; long epimeral setae. Four pairs of genital setae in a single line; crescent-shaped structure anterior to anal plate; anal plate with small sharp tip; aggenital and adanal setae more or less similar in length. Subcapitulum diarthric, three pairs of highly different setae a, m, h. Mentum complex.
Measurements. SEM: females 501 μm (489–515) × 270 μm (267–286). Light microscopy: females 506 μm (490–518) ×282 μm (276–301).
Colour. Specimens without cerotegument: females light brown to brown.
Cerotegument. Simple layer (±0.7 μm) (Figures
Integument. Microsculpture simple, covering entire body: irregular, small tuberculate (Figure
Setation. Setae in lanceolate (resembling leaf of Salix spp.), length 30 μm (28–34) (Figure
Prodorsum. Very complex. For proper understanding of structures, descriptions from various angles/views are included. Dorsal view (Figure
Frontal view (Figure
Lateral inclined view (Figures
Notogaster (Figure
Lateral region (Figures
Rugocepheus costaricensis sp. n. Adult, (with cerotegument) SEM. 43 lateral view 44 rostral zone 45le setae; 46 notogastral setae 47 notogastral cerotegumental layer, ventral view 48 anterior prodorsal zone 49 notogastral cerotegumental layer, ventrolateral view 50 cerotegumental layer 51 bothridial zone 52 ventrolateral inclined zone. Scale bars: 100 μm (43); 5 μm (44); 10 μm (45); 5 μm (46); 10 μm (47); 20 μm (48);10 μm (49); 2 μm (50); 20 μm (51); 50 μm (52).
Ventral region. Epimeral zone more or less smooth with large elevations and depressions. Paraxial zone of epimera 1 and 2 with longitudinal furrow; large paraxial depression behind bo.sj . Epimere 4 posterior border elevated. Anterior genital furrow (a.g.f) well visible (Figure
Legs (Table
Rugocepheus costaricensis sp. n. Adult, (with cerotegument) SEM. 53 ventral view 54ad2 setal zone 55 anal zone 56a subcapitular setae 58h subcapitular setae 57 subcapitulum 59 epimeral 3b setae 60 genital zone 61 aggenital, adanal setae. Scale bars: 100 μm (53); 5 μm (54); 20 μm (55); 2 μm (56); 5 μm (57); 5 μm (58); 5μm (59); 20 μm (60); 10 μm (61).
Legs | Femur | Genu | Tibia | Tarse | Claw |
---|---|---|---|---|---|
I | v (l); | (v) d | (l),v | (pv); s; (a); (u); (p); (it); (tc); (ft); ε | 1 |
σ | φ1, φ2 | ω1, ω2 | |||
II | (l) d | v l | v (v); | d Ad; (pv); s; (a); (u); (p); (it); (tc); (ft). | 1 |
σ | φ | ω1, ω2 | |||
III | l; | d | v (l) | (v) ft; (tc); (it); (p); (u); (a); s; (pv). | 1 |
σ | φ | ||||
IV | d | v d | (l). | v ft; (tc); (p); (u); (a); s; (pv | 1 |
φ |
Rugocepheus costaricensis sp. n. displays important differences to Rugocepheus joffrevillei Fernandez, Theron & Rollard, 2013 and R. formosus Mahunka, 2009. Principal differences: beak-shaped rostrum; distribution of furrows and elevated areas on dorsal zone of notogaster, central elevated area without setae; ventral zone with discidium differing in shape; genital and anal zone very different.
Using SEM allows significant progress in detailed descriptions, as the small body size, morphological characteristics, and complex topology makes Yoshiobodes a difficult genus to study. This complexity is compounded by brief, somewhat cryptic original descriptions and illustrations.
The most recent generic diagnosis by
For the purpose of this present paper, Yoshioiodes is considered only on the basis of
This work is based on research supported in part by the National Research Foundation of South Africa (UID) 85288. Any opinion, findings and conclusions or recommendations expressed in the material are those of the authors and therefore the NRF does not accept any liability in regard thereto.